BLASTX nr result
ID: Papaver30_contig00032394
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver30_contig00032394
(523 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_010268077.1| PREDICTED: uncharacterized protein LOC104605... 99 9e-19
ref|XP_010259720.1| PREDICTED: uncharacterized protein LOC104599... 99 9e-19
ref|XP_010922261.1| PREDICTED: uncharacterized protein LOC105045... 93 7e-17
ref|XP_008784402.1| PREDICTED: uncharacterized protein LOC103703... 89 1e-15
ref|XP_011079602.1| PREDICTED: uncharacterized protein LOC105163... 88 2e-15
ref|XP_002515331.1| conserved hypothetical protein [Ricinus comm... 88 2e-15
ref|XP_012837449.1| PREDICTED: uncharacterized protein LOC105957... 87 5e-15
ref|XP_006444441.1| hypothetical protein CICLE_v10022679mg [Citr... 86 8e-15
ref|XP_009391504.1| PREDICTED: uncharacterized protein LOC103977... 86 1e-14
ref|XP_008790857.1| PREDICTED: uncharacterized protein LOC103707... 84 4e-14
ref|XP_010544988.1| PREDICTED: uncharacterized protein LOC104817... 83 9e-14
ref|XP_013751985.1| PREDICTED: uncharacterized protein LOC106454... 82 1e-13
ref|XP_009142528.1| PREDICTED: uncharacterized protein LOC103866... 82 1e-13
emb|CDX80108.1| BnaA05g00480D [Brassica napus] 82 1e-13
ref|XP_004152233.1| PREDICTED: uncharacterized protein LOC101222... 82 1e-13
ref|XP_007051039.1| Uncharacterized protein TCM_004747 [Theobrom... 82 2e-13
ref|XP_002880306.1| binding protein [Arabidopsis lyrata subsp. l... 82 2e-13
ref|XP_002302741.1| hypothetical protein POPTR_0002s20100g [Popu... 82 2e-13
ref|NP_973709.1| uncharacterized protein [Arabidopsis thaliana] ... 82 2e-13
ref|XP_013634736.1| PREDICTED: uncharacterized protein LOC106340... 81 3e-13
>ref|XP_010268077.1| PREDICTED: uncharacterized protein LOC104605142 [Nelumbo nucifera]
Length = 146
Score = 99.4 bits (246), Expect = 9e-19
Identities = 55/112 (49%), Positives = 71/112 (63%)
Frame = -3
Query: 431 RRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXXXXG 252
RR+RFW I+ITPKL++ ++ SPKK+L +LRDAYV MMLGFA
Sbjct: 44 RRKRFWRIRITPKLRL-IRIPSPKKLLIRLRDAYVRMMLGFANTGVFSNGFGGGGAVG-- 100
Query: 251 DSGFMKPVRKEYDEKAIVEFYKSLMAQGQLMPRDAAKIVANGGGGGLVLCRR 96
+GF +P KEYDE +V+ YKSLMAQGQL+PRDA ++ A + CRR
Sbjct: 101 -AGFARPPLKEYDEAVLVQIYKSLMAQGQLLPRDAGRLAAE------ISCRR 145
>ref|XP_010259720.1| PREDICTED: uncharacterized protein LOC104599049 [Nelumbo nucifera]
Length = 147
Score = 99.4 bits (246), Expect = 9e-19
Identities = 57/115 (49%), Positives = 71/115 (61%)
Frame = -3
Query: 440 GNKRRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXX 261
G+ RRRRFW I++TPKL+ F SPKK+L +LRDAYV MMLGFA
Sbjct: 41 GSNRRRRFWRIRVTPKLRFF-HHLSPKKLLIRLRDAYVKMMLGFANSRVFSAGFGGGGAV 99
Query: 260 XXGDSGFMKPVRKEYDEKAIVEFYKSLMAQGQLMPRDAAKIVANGGGGGLVLCRR 96
G GF +P KEYDEK ++E YKSLMAQ QL+P+DA ++ A + CRR
Sbjct: 100 GAG-FGFGRPSLKEYDEKVLIEIYKSLMAQRQLVPQDAGRLAAQ------ISCRR 147
>ref|XP_010922261.1| PREDICTED: uncharacterized protein LOC105045608 [Elaeis guineensis]
Length = 149
Score = 93.2 bits (230), Expect = 7e-17
Identities = 52/108 (48%), Positives = 64/108 (59%)
Frame = -3
Query: 440 GNKRRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXX 261
G RRRRFW I++ PKL+ + SPK+ L ++RDAYV MML FA
Sbjct: 36 GGGRRRRFWRIRVVPKLRFLARMASPKRFLARIRDAYVRMMLSFASSGAIAAGYSGYGGA 95
Query: 260 XXGDSGFMKPVRKEYDEKAIVEFYKSLMAQGQLMPRDAAKIVANGGGG 117
+GF +P KEYDEK IVE Y+SL+AQG L+ AA VA GGGG
Sbjct: 96 AV--AGFGRPQLKEYDEKMIVEIYRSLVAQGPLI---AAAAVAGGGGG 138
>ref|XP_008784402.1| PREDICTED: uncharacterized protein LOC103703354 [Phoenix
dactylifera]
Length = 145
Score = 89.4 bits (220), Expect = 1e-15
Identities = 50/106 (47%), Positives = 62/106 (58%)
Frame = -3
Query: 431 RRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXXXXG 252
RRRRFW I++ P+L+ + SPK+ L ++RDAYV MML FA
Sbjct: 36 RRRRFWRIRVVPRLRFLARVASPKRFLGRIRDAYVRMMLSFASSGAIANGYTGYGGAAV- 94
Query: 251 DSGFMKPVRKEYDEKAIVEFYKSLMAQGQLMPRDAAKIVANGGGGG 114
+GF +P KEYDEK IVE YKSL+AQG LM +A GGGGG
Sbjct: 95 -TGFGRPQLKEYDEKMIVEIYKSLVAQGPLMAANAV-----GGGGG 134
>ref|XP_011079602.1| PREDICTED: uncharacterized protein LOC105163081 [Sesamum indicum]
Length = 151
Score = 88.2 bits (217), Expect = 2e-15
Identities = 56/117 (47%), Positives = 70/117 (59%), Gaps = 2/117 (1%)
Frame = -3
Query: 440 GNKRRRRFWGIKITPKLKIFLK-RNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXX 264
G+ RRRRFW IK+ P+LK+ LK R SPKK L LRDAYVNMM+ A
Sbjct: 42 GSARRRRFWRIKLMPRLKLKLKLRFSPKKFLLGLRDAYVNMMMKIANTRVIAGGFGGAYT 101
Query: 263 XXXGDSGFMKPVRKEYDEKAIVEFYKSL-MAQGQLMPRDAAKIVANGGGGGLVLCRR 96
++P+ KEYDE+ +VE YKS+ MAQGQL+PRDAA I + + CRR
Sbjct: 102 GSGVAEFGLRPM-KEYDERMLVEIYKSIVMAQGQLVPRDAACIDSE------IACRR 151
>ref|XP_002515331.1| conserved hypothetical protein [Ricinus communis]
gi|223545811|gb|EEF47315.1| conserved hypothetical
protein [Ricinus communis]
Length = 156
Score = 88.2 bits (217), Expect = 2e-15
Identities = 54/100 (54%), Positives = 62/100 (62%), Gaps = 1/100 (1%)
Frame = -3
Query: 440 GNKRRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXX 261
G++RR+ W IKI PKLKIF KR SPKK LRDAYVNMMLGFA
Sbjct: 48 GSRRRKMSWRIKIKPKLKIF-KRASPKKFFVWLRDAYVNMMLGFANSRVIGPGYGGGGGI 106
Query: 260 XXGDSGFMKPVRKEYDEKAIVEFYKSLMA-QGQLMPRDAA 144
+ F K KEYDEK I++ YKSL+A QGQL+P DAA
Sbjct: 107 A---TNFGKGPLKEYDEKMIIQIYKSLVAGQGQLVPHDAA 143
>ref|XP_012837449.1| PREDICTED: uncharacterized protein LOC105957994 [Erythranthe
guttatus] gi|604348619|gb|EYU46774.1| hypothetical
protein MIMGU_mgv1a015756mg [Erythranthe guttata]
Length = 147
Score = 87.0 bits (214), Expect = 5e-15
Identities = 58/116 (50%), Positives = 68/116 (58%), Gaps = 1/116 (0%)
Frame = -3
Query: 440 GNKRRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXX 261
G+ RRRRFW IK+T +LK+ L R SPKK L LRDAYVNMML A
Sbjct: 44 GSTRRRRFWRIKLTRRLKLKL-RFSPKKFLLGLRDAYVNMMLRIANTRVAGGGYGGDGV- 101
Query: 260 XXGDSGFMKPVRKEYDEKAIVEFYKSL-MAQGQLMPRDAAKIVANGGGGGLVLCRR 96
+GF KEYDE+ IVE YKS+ MAQGQ++PRDAA I G + CRR
Sbjct: 102 ----AGFGARPVKEYDERMIVEIYKSIVMAQGQIVPRDAAWI------GSEITCRR 147
>ref|XP_006444441.1| hypothetical protein CICLE_v10022679mg [Citrus clementina]
gi|568852744|ref|XP_006480031.1| PREDICTED:
uncharacterized protein LOC102622463 [Citrus sinensis]
gi|557546703|gb|ESR57681.1| hypothetical protein
CICLE_v10022679mg [Citrus clementina]
gi|641868401|gb|KDO87085.1| hypothetical protein
CISIN_1g031898mg [Citrus sinensis]
Length = 151
Score = 86.3 bits (212), Expect = 8e-15
Identities = 59/114 (51%), Positives = 67/114 (58%), Gaps = 3/114 (2%)
Frame = -3
Query: 431 RRRRF-WGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXXXX 255
RRRRF W IKI PKLKI + SPKK LRDAYV MM+GFA
Sbjct: 44 RRRRFSWRIKIKPKLKIS-RLPSPKKFFIWLRDAYVKMMMGFANSRVCASGGYGGGAIGS 102
Query: 254 GDS-GFMKPVRKEYDEKAIVEFYKSL-MAQGQLMPRDAAKIVANGGGGGLVLCR 99
+ GF K KEYDEK IV+ YKSL MAQGQL+PRDAA++ G V+CR
Sbjct: 103 NGAYGFGKGPLKEYDEKVIVDIYKSLVMAQGQLVPRDAARL------GSQVVCR 150
>ref|XP_009391504.1| PREDICTED: uncharacterized protein LOC103977655 [Musa acuminata
subsp. malaccensis]
Length = 135
Score = 85.9 bits (211), Expect = 1e-14
Identities = 46/99 (46%), Positives = 61/99 (61%)
Frame = -3
Query: 440 GNKRRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXX 261
G +RRRRFW IKI+P+L FL+ SP++IL ++RDAYV MMLGFA
Sbjct: 37 GGRRRRRFWRIKISPRLG-FLRAASPRRILARIRDAYVRMMLGFANCVAYGGYGYGGVEV 95
Query: 260 XXGDSGFMKPVRKEYDEKAIVEFYKSLMAQGQLMPRDAA 144
+G +P KEYDEK +VE YKSL+ +G ++ D A
Sbjct: 96 ----TGLARPALKEYDEKVLVEIYKSLVVRGPVVAADGA 130
>ref|XP_008790857.1| PREDICTED: uncharacterized protein LOC103707921 [Phoenix
dactylifera]
Length = 179
Score = 84.0 bits (206), Expect = 4e-14
Identities = 53/116 (45%), Positives = 63/116 (54%), Gaps = 1/116 (0%)
Frame = -3
Query: 440 GNKRRRRFWGIKITPKLKIFLKR-NSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXX 264
G R+RRFW I+I P FL R SPK+ L ++RDAYV MML FA
Sbjct: 68 GGGRKRRFWRIRIAPPRLRFLARVASPKRFLARIRDAYVRMMLSFASSGAIAAGYTEYGG 127
Query: 263 XXXGDSGFMKPVRKEYDEKAIVEFYKSLMAQGQLMPRDAAKIVANGGGGGLVLCRR 96
+GF +P KEYDEK IVE YKSL+AQG L+ AA A GG + RR
Sbjct: 128 DAA--AGFRRPQLKEYDEKMIVEIYKSLVAQGSLI--SAAAAAAAAGGDPQIAVRR 179
>ref|XP_010544988.1| PREDICTED: uncharacterized protein LOC104817473 [Tarenaya
hassleriana]
Length = 141
Score = 82.8 bits (203), Expect = 9e-14
Identities = 54/103 (52%), Positives = 62/103 (60%), Gaps = 2/103 (1%)
Frame = -3
Query: 431 RRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXXXXG 252
R+RRFW IKI PKL+I LK SPK++L LRDAYV MML A
Sbjct: 40 RKRRFWRIKIVPKLRI-LKTASPKRLLVWLRDAYVKMMLRLANSRVIGASGHVG------ 92
Query: 251 DSGFMK-PVRKEYDEKAIVEFYKS-LMAQGQLMPRDAAKIVAN 129
SGF PV KEYDEK +VE YKS LMAQG LM RD K+ ++
Sbjct: 93 -SGFGSGPVLKEYDEKMLVEIYKSILMAQGNLMHRDTPKLASD 134
>ref|XP_013751985.1| PREDICTED: uncharacterized protein LOC106454408 [Brassica napus]
Length = 141
Score = 82.4 bits (202), Expect = 1e-13
Identities = 52/102 (50%), Positives = 62/102 (60%), Gaps = 1/102 (0%)
Frame = -3
Query: 431 RRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXXXXG 252
R+RRFW IKI PKL++ LK+ SPKK+L LRDAYVNMML FA
Sbjct: 38 RKRRFWRIKIVPKLRV-LKKASPKKLLTWLRDAYVNMMLSFA--NSRVIESSYGFGEYGY 94
Query: 251 DSGFMKPVRKEYDEKAIVEFYKS-LMAQGQLMPRDAAKIVAN 129
SG +EYDEK +VE YKS LMAQG L+ RD K+ A+
Sbjct: 95 GSGL---ASREYDEKKLVEIYKSMLMAQGSLVHRDVPKLSAD 133
>ref|XP_009142528.1| PREDICTED: uncharacterized protein LOC103866375 [Brassica rapa]
Length = 141
Score = 82.4 bits (202), Expect = 1e-13
Identities = 52/102 (50%), Positives = 62/102 (60%), Gaps = 1/102 (0%)
Frame = -3
Query: 431 RRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXXXXG 252
R+RRFW IKI PKL++ LK+ SPKK+L LRDAYVNMML FA
Sbjct: 38 RKRRFWRIKIVPKLRV-LKKASPKKLLTWLRDAYVNMMLSFA--NSRVIESSYGFGEYGY 94
Query: 251 DSGFMKPVRKEYDEKAIVEFYKS-LMAQGQLMPRDAAKIVAN 129
SG +EYDEK +VE YKS LMAQG L+ RD K+ A+
Sbjct: 95 GSGL---ASREYDEKKLVEIYKSMLMAQGSLVHRDVPKLSAD 133
>emb|CDX80108.1| BnaA05g00480D [Brassica napus]
Length = 141
Score = 82.4 bits (202), Expect = 1e-13
Identities = 52/102 (50%), Positives = 62/102 (60%), Gaps = 1/102 (0%)
Frame = -3
Query: 431 RRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXXXXG 252
R+RRFW IKI PKL++ LK+ SPKK+L LRDAYVNMML FA
Sbjct: 38 RKRRFWRIKIVPKLRV-LKKASPKKLLTWLRDAYVNMMLSFA--NSRVIESSYGFGEYGY 94
Query: 251 DSGFMKPVRKEYDEKAIVEFYKS-LMAQGQLMPRDAAKIVAN 129
SG +EYDEK +VE YKS LMAQG L+ RD K+ A+
Sbjct: 95 GSGL---ASREYDEKKLVEIYKSMLMAQGSLVHRDVPKLSAD 133
>ref|XP_004152233.1| PREDICTED: uncharacterized protein LOC101222359 [Cucumis sativus]
gi|700197680|gb|KGN52838.1| hypothetical protein
Csa_4G002550 [Cucumis sativus]
Length = 154
Score = 82.4 bits (202), Expect = 1e-13
Identities = 45/90 (50%), Positives = 56/90 (62%)
Frame = -3
Query: 428 RRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXXXXGD 249
RRRFW IKI+PK + K SPKK+L +LRDAYVNMMLGFA G
Sbjct: 48 RRRFWRIKISPKFRFLRKIPSPKKLLLRLRDAYVNMMLGFANSRVFNTSYGGGAAVCDGI 107
Query: 248 SGFMKPVRKEYDEKAIVEFYKSLMAQGQLM 159
GF + + KEYDEK I+E YKS++A+ + M
Sbjct: 108 PGFGQRLAKEYDEKMILEIYKSVVAEQREM 137
>ref|XP_007051039.1| Uncharacterized protein TCM_004747 [Theobroma cacao]
gi|508703300|gb|EOX95196.1| Uncharacterized protein
TCM_004747 [Theobroma cacao]
Length = 142
Score = 82.0 bits (201), Expect = 2e-13
Identities = 51/102 (50%), Positives = 59/102 (57%), Gaps = 1/102 (0%)
Frame = -3
Query: 440 GNKRRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXX 261
G+ RRRRFW I++ KL+I SPKK LRDAYV MMLGFA
Sbjct: 39 GSTRRRRFWRIRVKAKLRI----PSPKKFFVWLRDAYVKMMLGFANSRMINTGYGGAIGD 94
Query: 260 XXGDSGFMKPVRKEYDEKAIVEFYKSL-MAQGQLMPRDAAKI 138
+ F K KEYDEK IVE YKSL M QGQL+PR+A K+
Sbjct: 95 GI--AAFGKGPMKEYDEKVIVEIYKSLVMGQGQLVPREAGKL 134
>ref|XP_002880306.1| binding protein [Arabidopsis lyrata subsp. lyrata]
gi|297326145|gb|EFH56565.1| binding protein [Arabidopsis
lyrata subsp. lyrata]
Length = 141
Score = 82.0 bits (201), Expect = 2e-13
Identities = 52/102 (50%), Positives = 62/102 (60%), Gaps = 1/102 (0%)
Frame = -3
Query: 431 RRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXXXXG 252
R+R+FW IKI PKL+ FLK++SPKK+L LRDAYVNMML A
Sbjct: 38 RKRQFWRIKIVPKLR-FLKKSSPKKLLTWLRDAYVNMMLRLA--NSRVVGSSYGYGEYGY 94
Query: 251 DSGFMKPVRKEYDEKAIVEFYKS-LMAQGQLMPRDAAKIVAN 129
SG KEYDEK +VE YKS LMAQG L+ RD K+ +N
Sbjct: 95 GSGL---ASKEYDEKKLVEIYKSILMAQGNLVHRDVPKLSSN 133
>ref|XP_002302741.1| hypothetical protein POPTR_0002s20100g [Populus trichocarpa]
gi|222844467|gb|EEE82014.1| hypothetical protein
POPTR_0002s20100g [Populus trichocarpa]
Length = 148
Score = 81.6 bits (200), Expect = 2e-13
Identities = 54/99 (54%), Positives = 63/99 (63%), Gaps = 2/99 (2%)
Frame = -3
Query: 437 NKRRRRF-WGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXX 261
++RRRRF W IK+ PKLK LK +SPK+ LRDAYV MMLGFA
Sbjct: 45 SRRRRRFAWRIKVKPKLKA-LKMSSPKRFFVWLRDAYVKMMLGFA-------NSRARGTA 96
Query: 260 XXGDSGFMKPVRKEYDEKAIVEFYKSL-MAQGQLMPRDA 147
GD +P+ KEYDEK I+E YKSL MAQGQL+PRDA
Sbjct: 97 GYGDGIGARPI-KEYDEKMIIEIYKSLVMAQGQLVPRDA 134
>ref|NP_973709.1| uncharacterized protein [Arabidopsis thaliana]
gi|50253464|gb|AAT71934.1| At2g47485 [Arabidopsis
thaliana] gi|51972104|gb|AAU15156.1| At2g47485
[Arabidopsis thaliana] gi|110738648|dbj|BAF01249.1|
hypothetical protein [Arabidopsis thaliana]
gi|330255755|gb|AEC10849.1| uncharacterized protein
AT2G47485 [Arabidopsis thaliana]
Length = 141
Score = 81.6 bits (200), Expect = 2e-13
Identities = 53/102 (51%), Positives = 61/102 (59%), Gaps = 1/102 (0%)
Frame = -3
Query: 431 RRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXXXXG 252
R+RRFW IKI PKL+I LK+ SPKK+L LRDAYVNMML A
Sbjct: 38 RKRRFWRIKIVPKLRI-LKKASPKKLLTWLRDAYVNMMLRLA--NSRVVGSSYGYGEYGY 94
Query: 251 DSGFMKPVRKEYDEKAIVEFYKS-LMAQGQLMPRDAAKIVAN 129
SG KEYDEK +VE YKS LMAQG L+ RD K+ +N
Sbjct: 95 GSGL---ASKEYDEKKLVEIYKSILMAQGNLVHRDVPKLSSN 133
>ref|XP_013634736.1| PREDICTED: uncharacterized protein LOC106340402 [Brassica oleracea
var. oleracea]
Length = 141
Score = 81.3 bits (199), Expect = 3e-13
Identities = 51/102 (50%), Positives = 62/102 (60%), Gaps = 1/102 (0%)
Frame = -3
Query: 431 RRRRFWGIKITPKLKIFLKRNSPKKILKKLRDAYVNMMLGFAXXXXXXXXXXXXXXXXXG 252
++RRFW IKI PKL++ LK+ SPKK+L LRDAYVNMML FA
Sbjct: 38 KKRRFWRIKIVPKLRV-LKKASPKKLLTWLRDAYVNMMLSFA--NSRVIESSYGFGEYGY 94
Query: 251 DSGFMKPVRKEYDEKAIVEFYKS-LMAQGQLMPRDAAKIVAN 129
SG +EYDEK +VE YKS LMAQG L+ RD K+ A+
Sbjct: 95 GSGL---ASREYDEKKLVEIYKSMLMAQGSLVHRDVPKLSAD 133