BLASTX nr result
ID: Papaver30_contig00000970
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver30_contig00000970
(678 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q7XB11.1|4OMT1_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-co... 434 e-119
gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353] 369 e-99
sp|Q7XB10.1|4OMT2_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-co... 369 e-99
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe... 321 2e-85
gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 321 3e-85
gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 294 3e-77
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc... 288 3e-75
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra... 286 8e-75
gb|AJD20227.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra... 244 4e-62
gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 241 2e-61
ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 231 3e-58
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica] 227 6e-57
ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 223 1e-55
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth... 216 1e-53
gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353] 213 7e-53
sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-meth... 213 7e-53
ref|XP_010271691.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 213 9e-53
gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyl... 213 1e-52
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr... 212 2e-52
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt... 211 3e-52
>sp|Q7XB11.1|4OMT1_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase 1; Short=4'-OMT1; Short=Ps4'OMT1;
AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase
gi|33413892|gb|AAP45313.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 1 [Papaver somniferum]
Length = 354
Score = 434 bits (1115), Expect = e-119
Identities = 215/225 (95%), Positives = 218/225 (96%)
Frame = -3
Query: 676 IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLN 497
IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYR+LRYLVHLN
Sbjct: 23 IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRLLRYLVHLN 82
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
ILGQQTCAAG DRVYSLKPVGTLLLKDSE SM PVILGLSQKDFLFVWNFVKEGLGTGST
Sbjct: 83 ILGQQTCAAGVDRVYSLKPVGTLLLKDSERSMAPVILGLSQKDFLFVWNFVKEGLGTGST 142
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGG 137
TAFEKA GMDMWKYLEV P+QS+LFDEGQAGETRLLTKTLL DCRDTFQGMDSLVDVGGG
Sbjct: 143 TAFEKAMGMDMWKYLEVNPNQSQLFDEGQAGETRLLTKTLLVDCRDTFQGMDSLVDVGGG 202
Query: 136 NGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMFMS 2
NGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNI KVPGDMFMS
Sbjct: 203 NGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNILKVPGDMFMS 247
>gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353]
Length = 359
Score = 369 bits (947), Expect = e-99
Identities = 174/225 (77%), Positives = 205/225 (91%)
Frame = -3
Query: 676 IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLN 497
IIYG+ADSLVLRC VEIGIADIIKNN+G+ITL++L +KLP++NV+SD LYR++RYLVHLN
Sbjct: 26 IIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVRYLVHLN 85
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
I+ Q+TC G ++VYSLKPVGTLLL+D+E SMVP+ILG++QKDF+ W+F+KEGLG GST
Sbjct: 86 IIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEGLGNGST 145
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGG 137
TAFEK GMD+WKYLE PDQS+LF+EG AGETRLLTKTL+EDCRDTFQG+DSLVD+GGG
Sbjct: 146 TAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQGLDSLVDIGGG 205
Query: 136 NGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMFMS 2
NGTTIKAI+EAFPHIKCTLYDLPHV+ANS D PNI KVPGDMF S
Sbjct: 206 NGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKS 250
>sp|Q7XB10.1|4OMT2_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase 2; Short=4'-OMT2; Short=Ps4'OMT2;
AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase
gi|33413894|gb|AAP45314.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 2 [Papaver somniferum]
gi|571330882|gb|AHF27397.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'- O-methyltransferase 2 [synthetic construct]
Length = 357
Score = 369 bits (947), Expect = e-99
Identities = 174/225 (77%), Positives = 205/225 (91%)
Frame = -3
Query: 676 IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLN 497
IIYG+ADSLVLRC VEIGIADIIKNN+G+ITL++L +KLP++NV+SD LYR++RYLVHLN
Sbjct: 26 IIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVRYLVHLN 85
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
I+ Q+TC G ++VYSLKPVGTLLL+D+E SMVP+ILG++QKDF+ W+F+KEGLG GST
Sbjct: 86 IIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEGLGNGST 145
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGG 137
TAFEK GMD+WKYLE PDQS+LF+EG AGETRLLTKTL+EDCRDTFQG+DSLVD+GGG
Sbjct: 146 TAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQGLDSLVDIGGG 205
Query: 136 NGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMFMS 2
NGTTIKAI+EAFPHIKCTLYDLPHV+ANS D PNI KVPGDMF S
Sbjct: 206 NGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKS 250
>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
californica]
Length = 353
Score = 321 bits (823), Expect = 2e-85
Identities = 154/226 (68%), Positives = 189/226 (83%), Gaps = 3/226 (1%)
Frame = -3
Query: 676 IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLN 497
IIYG+ADSLVLR VE+GIADIIKNNNGSIT+SEL SKLP+SNVNSDNLYR+LRYLVH+
Sbjct: 20 IIYGFADSLVLRSAVELGIADIIKNNNGSITVSELASKLPISNVNSDNLYRVLRYLVHMG 79
Query: 496 ILGQQTCAAGGDRV---YSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGT 326
IL + G + YSL+PVG+LL+KD+E +MVP++LG++Q+DF+ W+++KEGLG
Sbjct: 80 ILKETKSTINGGEIKKLYSLEPVGSLLVKDAERNMVPIVLGMTQQDFMIPWHYIKEGLGE 139
Query: 325 GSTTAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDV 146
GST AFEK GM +W+YLE P+Q LF+ G GETRLLTKTL+E C+DTF+G+ SLVDV
Sbjct: 140 GST-AFEKGMGMTLWEYLEGHPEQGHLFNVGMEGETRLLTKTLIESCKDTFEGLSSLVDV 198
Query: 145 GGGNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMF 8
GGGNGTTIKAI EAFPHIKC+LYDLPHV+A+S D PNI K+PGD+F
Sbjct: 199 GGGNGTTIKAISEAFPHIKCSLYDLPHVVADSHDLPNIEKIPGDIF 244
>gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 353
Score = 321 bits (822), Expect = 3e-85
Identities = 153/225 (68%), Positives = 193/225 (85%)
Frame = -3
Query: 676 IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLN 497
IIYG+ADSLVLRC VEIGIADIIK+NNGSI+++EL SKLP++NVNSDNLYR+LRYLVH+
Sbjct: 24 IIYGFADSLVLRCAVEIGIADIIKSNNGSISVTELASKLPITNVNSDNLYRVLRYLVHMG 83
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
IL ++ + ++YSL+PV TLLL+D+E SMVP+ILG++QKDF+ W+F+KEGLG T
Sbjct: 84 IL-KEVSDSNEVKLYSLQPVATLLLRDAERSMVPIILGMTQKDFMIPWHFMKEGLGN-DT 141
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGG 137
TAFEK GM +W+YLE P+QS LF+EG AGETRLLTK+L++ CRDTF+G+ SL DVGGG
Sbjct: 142 TAFEKGMGMTIWQYLEGHPEQSNLFNEGMAGETRLLTKSLIDGCRDTFEGLTSLCDVGGG 201
Query: 136 NGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMFMS 2
NGTTIK I++AFP IKC++YDLPHVIA+S +HPNI ++PGDMF S
Sbjct: 202 NGTTIKGIYDAFPQIKCSVYDLPHVIASSPEHPNIERIPGDMFKS 246
>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase [Coptis chinensis]
Length = 350
Score = 294 bits (753), Expect = 3e-77
Identities = 144/225 (64%), Positives = 182/225 (80%)
Frame = -3
Query: 676 IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLN 497
IIYG+ADSLVLRC VE+GI DI+ NNN + L++L SKLP+SNVN DNLYRILRYLV +
Sbjct: 21 IIYGFADSLVLRCAVELGIVDIVDNNNQPMALADLASKLPVSNVNCDNLYRILRYLVKME 80
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
IL + + G++ Y+L+P+ TLL ++++ SMVP+ILG++QKDF+ W+ +K+GL T
Sbjct: 81 ILRVEK-SDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHSMKDGLSDNGT 139
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGG 137
AFEKA GM +W+YLE PDQS+LF+EG AGETRLLT +L+ RD FQG+DSLVDVGGG
Sbjct: 140 -AFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQGIDSLVDVGGG 198
Query: 136 NGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMFMS 2
NGTT+KAI +AFPHIKCTL+DLPHVIANS DHPNI ++ GDMF S
Sbjct: 199 NGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKS 243
>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
gi|9711916|dbj|BAB08005.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
4'-O-methyltransferase [Coptis japonica]
Length = 350
Score = 288 bits (736), Expect = 3e-75
Identities = 143/225 (63%), Positives = 180/225 (80%)
Frame = -3
Query: 676 IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLN 497
IIYG+ADSLVLRC VE+GI DII NNN + L++L SKLP+S+VN DNLYRILRYLV +
Sbjct: 21 IIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNLYRILRYLVKME 80
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
IL + + G + Y+L+P+ TLL ++++ SMVP+ILG++QKDF+ W+ +K+GL T
Sbjct: 81 ILRVEK-SDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHSMKDGLSDNGT 139
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGG 137
AFEKA GM +W+YLE PDQS+LF+EG AGETRLLT +L+ RD FQG+DSLVDVGGG
Sbjct: 140 -AFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQGIDSLVDVGGG 198
Query: 136 NGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMFMS 2
NGTT+KAI +AFPHIKCTL+DLPHVIANS D PNI ++ GDMF S
Sbjct: 199 NGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFKS 243
>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
[Thalictrum flavum subsp. glaucum]
Length = 348
Score = 286 bits (732), Expect = 8e-75
Identities = 140/223 (62%), Positives = 174/223 (78%)
Frame = -3
Query: 676 IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLN 497
IIYG+ADSLVLRC VE+GI DII NNN I L++L SKLP+SNVN DNL+RILRYLV +
Sbjct: 22 IIYGFADSLVLRCAVELGIFDIIDNNNQPIPLADLASKLPISNVNLDNLFRILRYLVKME 81
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
+L D Y+L+P+ L+L++ + SMVP++LG++QKDF+ W+ +K+GL
Sbjct: 82 LLSY-----AADDKYALEPIAKLVLRNEKRSMVPMVLGMTQKDFMTPWHSMKDGLTDNGV 136
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGG 137
TAFEKA GM +W+YLE PDQS+LF+EG AGETRLLT +L+ +D FQ +DSLVDVGGG
Sbjct: 137 TAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSKDMFQSIDSLVDVGGG 196
Query: 136 NGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMF 8
NGTTIKAI AFPHIKCTL+DLPHVIANS DHPNI ++ GDMF
Sbjct: 197 NGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMF 239
>gb|AJD20227.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
[Sinopodophyllum hexandrum]
Length = 348
Score = 244 bits (623), Expect = 4e-62
Identities = 125/227 (55%), Positives = 162/227 (71%), Gaps = 2/227 (0%)
Frame = -3
Query: 676 IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLN 497
IIYG+ADSLVLRC VE+GI DII NN+ ITLS+L SKLPL++VN+ +LYRI+RYLVH+
Sbjct: 22 IIYGFADSLVLRCAVELGIPDIINNNHHPITLSDLASKLPLAHVNTHHLYRIMRYLVHMK 81
Query: 496 I--LGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTG 323
+ L +T YSL+ +GTLLL+D++ +MVP+ILG++QKDF+ W+++K+GL
Sbjct: 82 LVKLDNET------NQYSLESIGTLLLRDAKRNMVPIILGMTQKDFVTSWHYMKDGLSYN 135
Query: 322 STTAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVG 143
T AFEK GM +W+YL+ P+QS++F G AGE T + D S
Sbjct: 136 GT-AFEKGMGMTIWEYLQGHPEQSQJFXXGXAGEXXXXTXSXXXXXGDLXXXXTSXXXXX 194
Query: 142 GGNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMFMS 2
GGNGTTIK I +AFPHIKCTL+DLP+VIANS DHPNI +V GDMF S
Sbjct: 195 GGNGTTIKGISDAFPHIKCTLFDLPYVIANSYDHPNIERVAGDMFQS 241
>gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 348
Score = 241 bits (616), Expect = 2e-61
Identities = 113/223 (50%), Positives = 162/223 (72%)
Frame = -3
Query: 676 IIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLN 497
+IYG+A+SLVL+C +++ IAD I N+ ++LSEL SKLP+ VNSD LYR++RYLVH+
Sbjct: 17 LIYGFAESLVLKCAIQLEIADTIHNHGEPMSLSELASKLPVQPVNSDRLYRVMRYLVHMK 76
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
+ ++ + G+ YSL P L+K E SMV IL ++ KDFL W+ +K+GL +G
Sbjct: 77 LFNKEKTSINGEFKYSLAPPAKFLIKGWEKSMVASILAINDKDFLAPWHHLKDGL-SGDC 135
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGG 137
AFEKA G +W Y+ P++++LF+E A +TRL+T L+ DC+ F+G+++LVDVGGG
Sbjct: 136 DAFEKALGKSIWVYMSENPEKNQLFNEAMACDTRLVTSALVNDCQSVFKGINTLVDVGGG 195
Query: 136 NGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMF 8
GT +KAI +AFPHIKC++YDLPHVIA+S + PN+ K+ GDMF
Sbjct: 196 TGTAVKAISKAFPHIKCSIYDLPHVIADSPEIPNVVKIEGDMF 238
>ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 231 bits (589), Expect = 3e-58
Identities = 109/222 (49%), Positives = 161/222 (72%)
Frame = -3
Query: 673 IYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLNI 494
+YG+AD LVLRC +++GIADII +TLSEL +++P+ VN+D+L+R++RYLVH+ I
Sbjct: 18 VYGFADCLVLRCAIDLGIADIIHKQGEPLTLSELGAQIPVQPVNTDHLHRLMRYLVHMKI 77
Query: 493 LGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGSTT 314
++T G+ Y L P ++K + S+V +IL ++ KDF+ W+ +K+ L G T
Sbjct: 78 FTKETL--DGEARYGLAPPAKFIVKGWDKSIVSIILVVTDKDFMAPWHCLKDSL-CGEGT 134
Query: 313 AFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGGN 134
AFEKA G +W Y+ P+++KLF+EG A +T+LL L++DC+D FQG+ SLVDVGGG
Sbjct: 135 AFEKALGRSIWTYMADHPEKNKLFNEGMACDTKLLISALVQDCKDLFQGIMSLVDVGGGT 194
Query: 133 GTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMF 8
GT ++AI +AFPH+KCT+YDLPHVIA+S D+P + ++ GDMF
Sbjct: 195 GTAMRAIAKAFPHLKCTIYDLPHVIADSPDYPEVDRIAGDMF 236
>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
Length = 351
Score = 227 bits (578), Expect = 6e-57
Identities = 110/224 (49%), Positives = 157/224 (70%)
Frame = -3
Query: 673 IYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLNI 494
IYG+A+SL+L+ VE+ IAD I N+ +TLSEL SKLP+ V+ D LYR++RYLVH+ +
Sbjct: 21 IYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDRLYRVMRYLVHMKL 80
Query: 493 LGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGSTT 314
+Q YSL P L+K E SMV IL ++ KDF+ W+ +KEGL ++
Sbjct: 81 FKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLINDKDFMSPWHHLKEGL-VNNSN 139
Query: 313 AFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGGN 134
AF+KA G +W Y+ PD++K+F+EG A ++RL++ L+ +C + FQG+ +LVDVGGG
Sbjct: 140 AFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNECGNIFQGIQTLVDVGGGT 199
Query: 133 GTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMFMS 2
GT +KA+ +AFPHIKC++YDLPHVI++S D PNI K+ G+MF S
Sbjct: 200 GTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGEMFKS 243
>ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 223 bits (567), Expect = 1e-55
Identities = 105/221 (47%), Positives = 154/221 (69%)
Frame = -3
Query: 670 YGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLNIL 491
Y +AD+LVLRC +E GIADII +TL EL +++P+ VN+D+L+R++RY+VH+ I
Sbjct: 19 YDFADTLVLRCAIEFGIADIIHKQGEPLTLFELGAQIPVQPVNTDHLHRLMRYMVHMKIF 78
Query: 490 GQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGSTTA 311
++T GG+ Y L P G L+K + SM IL ++ +DF W+ +K+ L G TA
Sbjct: 79 TKETL--GGEEXYGLSPHGKFLVKGWDKSMASAILAITDEDFFAPWHCLKDVLA-GEGTA 135
Query: 310 FEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGGNG 131
FEKA G +W Y+ P+++KLF+E A +T +T L++DC+D FQG+ S+VDVGGG G
Sbjct: 136 FEKALGKSIWAYVADHPEKNKLFNEVMACDTSFITSVLIQDCKDVFQGIKSVVDVGGGTG 195
Query: 130 TTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMF 8
T ++ I +AFPH+KCT+YDLPHVIA+S D+P + ++ GDMF
Sbjct: 196 TAMRDIAKAFPHLKCTIYDLPHVIADSPDYPEVDRIAGDMF 236
>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase; Short=6-OMT
gi|9711914|dbj|BAB08004.1|
S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase [Coptis japonica]
Length = 347
Score = 216 bits (549), Expect = 1e-53
Identities = 104/222 (46%), Positives = 153/222 (68%)
Frame = -3
Query: 673 IYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLNI 494
IYG+A+SLVL+C V++ +A+II N+ S+TLSEL S+LP VN D LYR++RYLVH+ +
Sbjct: 18 IYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNEDALYRVMRYLVHMKL 77
Query: 493 LGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGSTT 314
+ + G+ Y L P L+K + MV IL ++ KDF+ W+++K+GL S T
Sbjct: 78 FTK--ASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPWHYLKDGLSGESGT 135
Query: 313 AFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGGN 134
AFEKA G ++W Y+ P++++LF+E A ++RL+ L+++C + F G+ +LVDVGGG
Sbjct: 136 AFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIFNGITTLVDVGGGT 195
Query: 133 GTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMF 8
GT ++ I AFPHIKCT+YDLPHVIA+S + + V GDMF
Sbjct: 196 GTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMF 237
>gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353]
Length = 348
Score = 213 bits (543), Expect = 7e-53
Identities = 112/226 (49%), Positives = 154/226 (68%), Gaps = 2/226 (0%)
Frame = -3
Query: 673 IYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSN-VNSDNLYRILRYLVHLN 497
IYG+A+SLVL+C V++ IA+ + NN ++LSEL SKLP++ VN D L+RI+RYLVH+
Sbjct: 19 IYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNEDRLFRIMRYLVHME 78
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
+ + YSL P LL+ E SMV IL ++ KDFL W+ + +GL TG+
Sbjct: 79 LFKIDATT----QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWHHLGDGL-TGNC 133
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQ-GMDSLVDVGG 140
AFEKA G +W Y+ V P++++LF+ A +TRL+T L +C+ F G+ +LVDVGG
Sbjct: 134 DAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKSIFSDGISTLVDVGG 193
Query: 139 GNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMFMS 2
G GT +KAI +AFP IKCT+YDLPHVIA+S + PNI K+ GDMF S
Sbjct: 194 GTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKS 239
>sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase;
Short=Ps6OMT gi|33286374|gb|AAQ01669.1|
(R,S)-norcoclaurine 6-O-methyltransferase [Papaver
somniferum] gi|571330878|gb|AHF27395.1|
(R,S)-norcoclaurine 6-O-methyltransferase [synthetic
construct]
Length = 346
Score = 213 bits (543), Expect = 7e-53
Identities = 112/226 (49%), Positives = 154/226 (68%), Gaps = 2/226 (0%)
Frame = -3
Query: 673 IYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSN-VNSDNLYRILRYLVHLN 497
IYG+A+SLVL+C V++ IA+ + NN ++LSEL SKLP++ VN D L+RI+RYLVH+
Sbjct: 19 IYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNEDRLFRIMRYLVHME 78
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
+ + YSL P LL+ E SMV IL ++ KDFL W+ + +GL TG+
Sbjct: 79 LFKIDATT----QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWHHLGDGL-TGNC 133
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQ-GMDSLVDVGG 140
AFEKA G +W Y+ V P++++LF+ A +TRL+T L +C+ F G+ +LVDVGG
Sbjct: 134 DAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKSIFSDGISTLVDVGG 193
Query: 139 GNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMFMS 2
G GT +KAI +AFP IKCT+YDLPHVIA+S + PNI K+ GDMF S
Sbjct: 194 GTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKS 239
>ref|XP_010271691.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 351
Score = 213 bits (542), Expect = 9e-53
Identities = 105/224 (46%), Positives = 154/224 (68%), Gaps = 2/224 (0%)
Frame = -3
Query: 673 IYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLNI 494
+YG+AD+LVLRC +E+GIADII +TLSEL +++PL +VN+D+L+R++RYLVH+ +
Sbjct: 18 VYGFADTLVLRCAIELGIADIIHKQGEPLTLSELGAQIPLKSVNTDHLHRLMRYLVHMKL 77
Query: 493 LGQQTCAAGGDRVYSLKPVGTLLLKDSETS-MVPVILGLSQKDFLFVWNFVKEGL-GTGS 320
++T G+ Y L P LL+K E + +I G++ KDF+ W+ +K+ L G G
Sbjct: 78 FTKETL--DGEARYGLAPPAKLLVKWWEDKGLASIIFGITDKDFIAPWHHLKDSLAGDGE 135
Query: 319 TTAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGG 140
T FEK G + Y+ ++S LF+E +TRL T L++D +D FQG+ SLVDVGG
Sbjct: 136 ETTFEKVLGKSISTYMADHLEKSMLFNESMVHDTRLFTSVLIQDFKDVFQGIKSLVDVGG 195
Query: 139 GNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMF 8
G+GT + AI +AFPH+KCT+Y LPHVIA+S D+P + ++ GDMF
Sbjct: 196 GSGTDMGAIAKAFPHLKCTIYGLPHVIADSPDYPEVDRISGDMF 239
>gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyllum hexandrum]
Length = 350
Score = 213 bits (541), Expect = 1e-52
Identities = 104/223 (46%), Positives = 151/223 (67%), Gaps = 1/223 (0%)
Frame = -3
Query: 673 IYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSN-VNSDNLYRILRYLVHLN 497
IYG+A+SLVL+C VE+ A+II N+ +TLSEL S+LP+ VN+++LYR++RYLVH+N
Sbjct: 18 IYGFAESLVLKCAVELDFANIIHNHGKPMTLSELASQLPVMQPVNTNSLYRVMRYLVHIN 77
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
I + G+ Y L L+K + MV ILG++ K F+ W ++K+ L G+
Sbjct: 78 IFTKTLDENDGETKYGLAAPAKFLVKGWDNCMVGSILGITDKVFMEPWYYIKDELAPGTG 137
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGGG 137
TAFE A G D+W+Y+ P+++KLF+ A +T ++ L+ +C+D F G+ +LVDVGGG
Sbjct: 138 TAFELALGKDIWEYMGENPEKNKLFNAAMACDTSMIMSALISECKDKFNGIRTLVDVGGG 197
Query: 136 NGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMF 8
GT + I AFP+IKCT+YDLPHVIA+S +P I +V GDMF
Sbjct: 198 TGTAARNIARAFPNIKCTVYDLPHVIADSPVYPEINRVSGDMF 240
>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
glaucum]
Length = 350
Score = 212 bits (539), Expect = 2e-52
Identities = 106/224 (47%), Positives = 152/224 (67%), Gaps = 2/224 (0%)
Frame = -3
Query: 673 IYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNVNSDNLYRILRYLVHLNI 494
IYG+ADSLVL+ V++ +A+II N+ +TLSEL LP VN D LYR+LRYLVH+ +
Sbjct: 19 IYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQDALYRVLRYLVHMKL 78
Query: 493 LGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEG-LGTGST 317
+ + G+ Y L P L+K + M+ IL ++ KDF+ W+++KEG L GST
Sbjct: 79 FTKSSI--DGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPWHYLKEGILNDGST 136
Query: 316 -TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQGMDSLVDVGG 140
TAFEKA G ++W Y+ P++++LF+EG A +TRL+ L+++C F G+ ++VDVGG
Sbjct: 137 STAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSSMFDGITTIVDVGG 196
Query: 139 GNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMF 8
G GT ++ I +AFPHIKCT+YDLPHVIA+S + I + GDMF
Sbjct: 197 GTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMF 240
>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase
[Papaver somniferum]
Length = 346
Score = 211 bits (537), Expect = 3e-52
Identities = 111/226 (49%), Positives = 153/226 (67%), Gaps = 2/226 (0%)
Frame = -3
Query: 673 IYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSN-VNSDNLYRILRYLVHLN 497
IYG+A+SLVL+C V++ IA+ + NN ++LSEL SKLP++ VN D L+RI+RYLVH+
Sbjct: 19 IYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNEDRLFRIMRYLVHME 78
Query: 496 ILGQQTCAAGGDRVYSLKPVGTLLLKDSETSMVPVILGLSQKDFLFVWNFVKEGLGTGST 317
+ + YSL P LL+ E SMV IL ++ KDFL W+ + +GL TG+
Sbjct: 79 LFKIDATT----QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWHHLGDGL-TGNC 133
Query: 316 TAFEKATGMDMWKYLEVTPDQSKLFDEGQAGETRLLTKTLLEDCRDTFQ-GMDSLVDVGG 140
AFEKA G +W Y+ P++++LF+ A +TRL+T L +C+ F G+ +LVDVGG
Sbjct: 134 DAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSIFSDGISTLVDVGG 193
Query: 139 GNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNIRKVPGDMFMS 2
G GT +KAI +AFP IKCT+YDLPHVIA+S + PNI K+ GDMF S
Sbjct: 194 GTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKS 239