BLASTX nr result
ID: Papaver29_contig00018496
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver29_contig00018496
(1004 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 610 e-172
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 605 e-170
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 602 e-169
sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 532 e-148
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 504 e-140
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 503 e-139
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 500 e-139
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 481 e-133
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 476 e-131
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 459 e-126
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 298 5e-78
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss... 298 5e-78
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum] 292 3e-76
ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 287 8e-75
ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 287 1e-74
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 286 2e-74
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 286 2e-74
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera] 285 3e-74
ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 282 3e-73
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 278 6e-72
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 610 bits (1573), Expect = e-172
Identities = 305/334 (91%), Positives = 315/334 (94%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
LF+LKLGSETVV+ASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECN+TWK
Sbjct: 62 LFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 121
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
KLRKVCR ELFTQKMIESQA+IRESKAMEMV++LK+N+G+EVKIVEVVFGTLVNIFGNLI
Sbjct: 122 KLRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLI 181
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 463
FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI
Sbjct: 182 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 241
Query: 462 YSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 283
YSVWGAMLKERKI KLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW
Sbjct: 242 YSVWGAMLKERKIAKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 301
Query: 282 ALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 103
ALSELTKNP+VTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKE
Sbjct: 302 ALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRR 361
Query: 102 XLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
LETCKVLNY IPKECQIMVNAWGIGRDPKRWTD
Sbjct: 362 ALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTD 395
>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3
gi|6176562|gb|AAF05621.1|AF191772_1
(S)-N-methylcoclaurine 3'-hydroxylase [Papaver
somniferum]
Length = 481
Score = 605 bits (1561), Expect = e-170
Identities = 304/334 (91%), Positives = 310/334 (92%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
LFSLKLGSETVV+ASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECN+TWK
Sbjct: 57 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 116
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
KLRKVCRTELFTQKMIESQA++RESKAMEMV+YLKKN GNEVKI EVVFGTLVNIFGNLI
Sbjct: 117 KLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 176
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 463
FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLG+FDLFGQ KDVADCLQGI
Sbjct: 177 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGI 236
Query: 462 YSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 283
YSVWGAMLKERKI K HNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW
Sbjct: 237 YSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 296
Query: 282 ALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 103
ALSELTKNP VTANMR ELLSVVGKRPVKESDIPNMPYLQAFVKE
Sbjct: 297 ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRR 356
Query: 102 XLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
LETCKVLNY IPKECQIMVNAWGIGRDPKRWTD
Sbjct: 357 ALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTD 390
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 602 bits (1552), Expect = e-169
Identities = 302/334 (90%), Positives = 309/334 (92%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
LFSLKLGSETVV+ASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECN+TWK
Sbjct: 57 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWK 116
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
KLRKVCRT+LFTQKMIESQA++RESKAMEMV+YLKKN GNEVKI EVVFGTLVNIFGNLI
Sbjct: 117 KLRKVCRTDLFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLI 176
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 463
FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLG+FDLFGQ KDVADCLQGI
Sbjct: 177 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGI 236
Query: 462 YSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 283
YSVWGAMLKE KI K HNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW
Sbjct: 237 YSVWGAMLKESKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 296
Query: 282 ALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 103
ALSELTKNP VTANMR ELLSVVGKRPVKESDIPNMPYLQAFVKE
Sbjct: 297 ALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRR 356
Query: 102 XLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
LETCKVLNY IPKECQIMVNAWGIGRDPKRWTD
Sbjct: 357 ALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTD 390
>sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
AltName: Full=Cytochrome P450 80BX
gi|388571234|gb|AFK73713.1| cytochrome P450 [Papaver
somniferum]
Length = 440
Score = 532 bits (1371), Expect = e-148
Identities = 261/335 (77%), Positives = 292/335 (87%), Gaps = 1/335 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
LF+LKLG++TVV+ASTPLAASE+LK HDR L GRYVFQSFRVK HVENSIVW+ECN+TWK
Sbjct: 63 LFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNETWK 122
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
KLRKVCRT+LFTQKMIE+QA++RE K MEMVKYLKKNEG EVKIVEV+FGTLVN+FGNLI
Sbjct: 123 KLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNLI 182
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 463
FSQ+IFKLGDESSGS+EMK+H+WRMLELGNS NP DY P LG DLFGQRKDVADCLQG+
Sbjct: 183 FSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQGV 242
Query: 462 YSVWGAMLKERKIPKLH-NNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIE 286
Y VWGAMLKER+I K N KNDFV++LLDSGLDDQQIN+LL+++F AGTET+ASTIE
Sbjct: 243 YGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTASTIE 302
Query: 285 WALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXX 106
WAL+ELTKNP VTA++RSELLSVVGKR VKESDI N+PYLQAFVKE
Sbjct: 303 WALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLIPR 362
Query: 105 XXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
LETC+VLNY IPKECQIMVNAWGIGRDPK WTD
Sbjct: 363 RALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTD 397
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1
gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 487
Score = 504 bits (1298), Expect = e-140
Identities = 246/335 (73%), Positives = 285/335 (85%), Gaps = 1/335 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
+F+LK+G+ETVV+AST AASEILKTHDR+LS RYVFQSFRVK HVENSIVWS+C +TWK
Sbjct: 62 IFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWK 121
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
LRKVCRTELFTQKMIESQA +RE K EMV+YL K +G EVKIVEV+FGTLVNIFGNLI
Sbjct: 122 NLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLI 181
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 463
FSQNIF+LGD +SGS E KE+LWRMLELGNSTNPADYFP LG+FDLFGQRK+VA+CL+GI
Sbjct: 182 FSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGI 241
Query: 462 YSVWGAMLKERKIPKLHNNSK-KNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIE 286
Y++WGAML+ERK+ K + K KNDFV++ LDSGL+D QINALLME+FGAGTETSASTIE
Sbjct: 242 YAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIE 301
Query: 285 WALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXX 106
WA++ELTKNP +TA +RSE+ +VVG+R VKESD PN+PYL+A VKE
Sbjct: 302 WAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPR 361
Query: 105 XXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
LETC +LNY IPK+CQIMVNAWGIGRDPK WTD
Sbjct: 362 RALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTD 396
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 503 bits (1294), Expect = e-139
Identities = 245/335 (73%), Positives = 283/335 (84%), Gaps = 1/335 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
+F+LK+G+ETVV+AST AASEILKTHDR+LS RYVFQSFRVK HVENSIVWS+C +TWK
Sbjct: 63 IFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWK 122
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
LRKVCRTELFTQKMIESQA +RE K EMV+YL K +G EVKIVEV+FGTLVNIFGNLI
Sbjct: 123 NLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLI 182
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 463
FSQNIF+LGD +SGS E KE+LWRMLELGNSTNPADYFP LG+FDLFGQRK+VA+CL+GI
Sbjct: 183 FSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGI 242
Query: 462 YSVWGAMLKERKI-PKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIE 286
Y++WGAML+ERK+ K+ KNDFV++ LDSGL+D QINALLME+FGAGTETSASTIE
Sbjct: 243 YAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIE 302
Query: 285 WALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXX 106
WA++ELTKNP +TA +RSEL +VVG+R VKESD PN+PYL+A VKE
Sbjct: 303 WAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPR 362
Query: 105 XXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
LETC +LNY IPK+CQIMVNAWGIGRDPK W D
Sbjct: 363 RALETCTILNYTIPKDCQIMVNAWGIGRDPKTWID 397
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2
gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 488
Score = 500 bits (1287), Expect = e-139
Identities = 244/335 (72%), Positives = 282/335 (84%), Gaps = 1/335 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
+F+LK+G+ETVV+AST AASEILKTHDR+LS RYVFQSFRVK HVENSIVWS+C +TWK
Sbjct: 63 IFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWK 122
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
LRKVCRTELFTQKMIESQA +RE K EMV+YL K +G EVKIVEV+FGTLVNIFGNLI
Sbjct: 123 NLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLI 182
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 463
FSQNIF+LG +SGS E KE+LWRMLELGNSTNPADYFP LG+FDLFGQRK+VA+CL+GI
Sbjct: 183 FSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGI 242
Query: 462 YSVWGAMLKERKI-PKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIE 286
Y++WGAML+ERK+ K+ KNDFV++ LDSGL+D QINALLME+FGAGTETSASTIE
Sbjct: 243 YAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIE 302
Query: 285 WALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXX 106
WA++ELTKNP +TA +RSEL +VVG+R VKESD PN+PYL+A VKE
Sbjct: 303 WAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPR 362
Query: 105 XXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
LETC +LNY IPK+CQIMVNAWGIGRDPK W D
Sbjct: 363 RALETCTILNYTIPKDCQIMVNAWGIGRDPKTWID 397
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2 [Coptis japonica]
gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 481 bits (1237), Expect = e-133
Identities = 236/334 (70%), Positives = 278/334 (83%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
LFSLKLGS+TVV+AS+P AA+EILKTHD++LSGRYVFQSFRVKEHVENSIVWSECND WK
Sbjct: 63 LFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWK 122
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
LRKVCRTELFT KMIESQ++IRE+KA EMVK+L+ EG VKIVEVVFGTLVNIFGNLI
Sbjct: 123 LLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLI 182
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 463
FS+++F L D + GSVE+KEHLW++L++GNSTNPADYFP +G+ DLFGQR+ VA+ LQ I
Sbjct: 183 FSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQI 242
Query: 462 YSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 283
Y VWG MLKER+ K + KNDFV++LL++GLDDQ+INALLME+FGAGTETSASTIEW
Sbjct: 243 YDVWGVMLKERRGTK--GSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEW 300
Query: 282 ALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 103
A++ELTK P V + +R EL++VVG VKESD+P++PYLQAFVKE
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRR 360
Query: 102 XLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
LETC V+NY IPKECQIMVNAW IGRDPK W D
Sbjct: 361 ALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDD 394
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 476 bits (1225), Expect = e-131
Identities = 232/334 (69%), Positives = 275/334 (82%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
LFSLKLGS+TVV+AS+P AA+E+LKTHD++LSGRYVFQSFRVKEHVENSIVWSECND WK
Sbjct: 63 LFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWK 122
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
LRKVCRTELFT KMIESQ++IRE KA EMVK+L+ EG VKIVEVVFGTLVNIFGNLI
Sbjct: 123 LLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLI 182
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 463
FS+++F L D + GSVE+KEHLW++LE+GNSTNPADYFP +G++DLFGQR+ VAD LQ I
Sbjct: 183 FSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQI 242
Query: 462 YSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 283
Y VWG LKER+ + + NDFV++LL++GL+DQ+INALLME+FG GTETSASTIEW
Sbjct: 243 YDVWGINLKERR--GMKGSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEW 300
Query: 282 ALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 103
A++ELTK P V + +R EL++VVG VKESD+P++PYLQAFVKE
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRR 360
Query: 102 XLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
LETC V+NY IPKECQIMVNAW IGRDPK W D
Sbjct: 361 ALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDD 394
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 459 bits (1180), Expect = e-126
Identities = 224/334 (67%), Positives = 274/334 (82%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
LF+LKLGS+TVV+AS+P AA+EILKT D++LSGRYVFQSFRV EHV NSIVWSECN+ WK
Sbjct: 64 LFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNENWK 123
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
LRKVCRTELF+ KMIESQA IRE+KA++MV++L+K E EVKIVEVVF TLVNIFGNLI
Sbjct: 124 LLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFGNLI 183
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 463
FS+++F L D + GS E+KEHLW++L++GNSTNPADYFP +G+FDLFGQR++VA L+ I
Sbjct: 184 FSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVLKQI 243
Query: 462 YSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 283
Y VWG MLKER+ H ++NDF +LL++GLDDQ+INALLME+FGAGTET+ASTIEW
Sbjct: 244 YDVWGVMLKERRSITGH---RENDFANVLLNAGLDDQKINALLMELFGAGTETTASTIEW 300
Query: 282 ALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXX 103
A++ELT N V + +R+EL++VVG + VKES+IP++PYLQA VKE
Sbjct: 301 AITELTNNLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKETLRLHPPTPLLLPRR 360
Query: 102 XLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
LETCKV+NY IPKECQIMVNAW IGRDPK W D
Sbjct: 361 ALETCKVMNYTIPKECQIMVNAWAIGRDPKTWDD 394
>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 298 bits (763), Expect = 5e-78
Identities = 149/333 (44%), Positives = 221/333 (66%), Gaps = 1/333 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
L LK G E VV+AS+ +AA E+LK DR+LSGR+ S R+K ++E+S+VW++C D WK
Sbjct: 63 LMLLKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDYWK 122
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
+RKV RTELF+ KM++ QA RE K E++K+L + EG +V +V+FG+++NI G LI
Sbjct: 123 MVRKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGALI 182
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGI 463
FS++++ D + ++ MK + +++ L N AD +P LG D G RK A C++ +
Sbjct: 183 FSKDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDFQGLRKASAACVKRM 242
Query: 462 YSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEW 283
W A++K+R + +++ KNDF+++LLDSG D QI+A+L+E FG G++TS STIEW
Sbjct: 243 NESWAAIVKQR---RKNDDHSKNDFLQVLLDSGFSDPQIDAMLLETFGPGSDTSTSTIEW 299
Query: 282 ALSELTKNPDVTANMRSELLSVVGK-RPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXX 106
A++EL +NP+ +R EL V+ + VKESD+PN+PYL A VKE
Sbjct: 300 AMAELLRNPEKLVKVREELDRVIRRSNNVKESDLPNLPYLHACVKETLRLHPPVTFLLPH 359
Query: 105 XXLETCKVLNYIIPKECQIMVNAWGIGRDPKRW 7
+ETC+++NY IPK CQ+MVN + IGRD K W
Sbjct: 360 RAMETCQMMNYTIPKGCQLMVNTYAIGRDSKTW 392
>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 298 bits (763), Expect = 5e-78
Identities = 147/331 (44%), Positives = 214/331 (64%)
Frame = -3
Query: 993 LKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLR 814
L LG++ V+AST AA EILKTHDR+ S R++ SFR+K H++ S+VWS+C D WK LR
Sbjct: 69 LWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLR 128
Query: 813 KVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLIFSQ 634
K+ RTE+F+ KM+++Q+ +RE K E++ +L+ EG VKI + VFGTL+NI GN++FS+
Sbjct: 129 KIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSK 188
Query: 633 NIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSV 454
++F GDE+ ++ + ML +G N A+++P L DL G +K + + +
Sbjct: 189 DVFVYGDETDKG-GIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRVMKM 247
Query: 453 WGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 274
W +KER K + N + D +++LL + +D QINAL +E FG G+ETS++TIEW ++
Sbjct: 248 WEGTVKER---KANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIA 304
Query: 273 ELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXLE 94
EL K+P A +R EL VVG +KESD+P +PYLQA +KE E
Sbjct: 305 ELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPFLLPRRAAE 364
Query: 93 TCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
TC+V+ Y IPK Q++VNA+ IGRDPK W D
Sbjct: 365 TCEVMGYTIPKNSQVLVNAYAIGRDPKSWKD 395
>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 292 bits (747), Expect = 3e-76
Identities = 147/331 (44%), Positives = 216/331 (65%)
Frame = -3
Query: 993 LKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLR 814
L LG++ +AST AA EILKTHDR SGR V SFR+K H++ S+VW++C++ WK LR
Sbjct: 67 LWLGTQPTAVASTTEAAMEILKTHDRSFSGRAVRMSFRLKHHIKYSLVWADCSEYWKLLR 126
Query: 813 KVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLIFSQ 634
K+ RTE+F+ KM++ Q +RE K E+V++LK EG VKI ++VFGTL+NI GN+IFS+
Sbjct: 127 KIVRTEIFSPKMLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSK 186
Query: 633 NIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSV 454
++F+ + +M+ + ML +G + N A+++P FDL G +K + +
Sbjct: 187 DVFRY--VGADGDKMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKM 244
Query: 453 WGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 274
W ++ERK+ + N + D +++LL + +D QINA+ +E FG G+ETS++T EWAL+
Sbjct: 245 WKGTVEERKVKR---NEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALA 301
Query: 273 ELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXLE 94
EL KNP + A +R EL VVGK VKESD+P++PYLQA +KE +E
Sbjct: 302 ELIKNPHILAKVRQELDQVVGKSTVKESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVE 361
Query: 93 TCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
TC V+ Y IPK+ Q++VN + IGRDPK W +
Sbjct: 362 TCPVMGYTIPKDTQVLVNIYAIGRDPKTWKE 392
>ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 486
Score = 287 bits (735), Expect = 8e-75
Identities = 144/334 (43%), Positives = 218/334 (65%), Gaps = 2/334 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWK 823
L + G E VV+AS +AA E+LK DRVLSGR+ S RVK ++E S+VW++C D WK
Sbjct: 63 LMLFRFGFENVVVASNHVAAMEVLKNQDRVLSGRFKANSVRVKGYIEYSMVWADCTDYWK 122
Query: 822 KLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFGNLI 643
+RK+ RTELF+ KM++ A +RE K E++K+L++ EG EV V+V+FG ++N+ G LI
Sbjct: 123 MVRKILRTELFSTKMLDVHAHVREEKVSELMKFLRRKEGEEVNFVDVIFGCILNMLGALI 182
Query: 642 FSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFP-FLGRFDLFGQRKDVADCLQG 466
+S++++ D++ ++ MK + +++ L + N AD +P F D G RK+ A C++
Sbjct: 183 YSKDVYDFEDKTDINLGMKGMIRQLMILAATPNIADLYPIFFDGSDFQGLRKESAACVKR 242
Query: 465 IYSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIE 286
+ W A++ ER + +N+ KND +++LLDSG D QI+A+ +E FG G++TSASTIE
Sbjct: 243 MSESWAAIINER---RKNNDHTKNDLLQVLLDSGFSDPQIDAIFLETFGPGSDTSASTIE 299
Query: 285 WALSELTKNPDVTANMRSELLSVVGK-RPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXX 109
WAL+EL +NP+ + EL V+G+ VK+SD+PN+PYL A VKE
Sbjct: 300 WALAELLRNPEKLVKLHEELDRVIGRNNTVKDSDLPNLPYLHACVKETLRLHPPVPFLIP 359
Query: 108 XXXLETCKVLNYIIPKECQIMVNAWGIGRDPKRW 7
LE+C+V+NY IPK +++VN + IGRDP W
Sbjct: 360 HIALESCEVMNYTIPKGSEVLVNLYAIGRDPTTW 393
>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223549945|gb|EEF51432.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 287 bits (734), Expect = 1e-74
Identities = 141/338 (41%), Positives = 214/338 (63%), Gaps = 4/338 (1%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN--SIVWSE-CND 832
L SL+LG++ +V+ASTP +A+E+LK HDR+LS R++ ++ + H+ +IVW+ CND
Sbjct: 70 LISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHILERVAIVWNPACND 129
Query: 831 TWKKLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFG 652
WK LR +CRTELF+ K IESQA +RE K EM+++L +G V + EVVFGT+ N
Sbjct: 130 HWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEVVFGTIFNTIS 189
Query: 651 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 472
+L+FS ++ ++ G +K LW M+E+ S N A+++P L D G ++ + CL
Sbjct: 190 HLLFSTDLIGFENQIGG---VKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMTKCL 246
Query: 471 QGIYSVWGAMLKERKIPKLHNNSK-KNDFVEILLDSGLDDQQINALLMEIFGAGTETSAS 295
+ ++ VW +KER+ H+++ K DF++I L +G DD QIN L+ME+ AGT+T+++
Sbjct: 247 KEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLSAGTDTTST 306
Query: 294 TIEWALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXX 115
++EWA++EL KN + +R EL + K P+KES + +PYL A VKE
Sbjct: 307 SVEWAMAELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACVKETLRLHPPAPFL 366
Query: 114 XXXXXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
E C+V+NY IPK+ Q++VN W IGRDP W D
Sbjct: 367 IPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWED 404
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 286 bits (731), Expect = 2e-74
Identities = 146/337 (43%), Positives = 209/337 (62%), Gaps = 3/337 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN--SIVWS-ECND 832
L SL+LG++ +V+AS+P AA E+LKTHDR LSGRY+ S VK N SIV++ +CN
Sbjct: 612 LMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNT 671
Query: 831 TWKKLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFG 652
WK LR +CR ELF+ K +ESQ ++RE K E+V++L EG VK++++VF T+ NI
Sbjct: 672 NWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILS 731
Query: 651 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 472
N FS ++ DE +K+ + + E G + N +DY+P LG D+ G + +
Sbjct: 732 NKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMF 791
Query: 471 QGIYSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 292
+ I + W +LKER+ + N S DF+E LL+ G +D QIN +++E+F AG ETS+ T
Sbjct: 792 ERIPTTWEDILKERRTQR-SNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLT 850
Query: 291 IEWALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 112
+EWA++EL +N D +R EL +VG+ PV+ES +P +PYLQA VKE
Sbjct: 851 VEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRLHPPAPLLL 910
Query: 111 XXXXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
ETC+V+ Y IPK+ QI VN W + RDPK W D
Sbjct: 911 PHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDD 947
Score = 282 bits (721), Expect = 3e-73
Identities = 142/337 (42%), Positives = 209/337 (62%), Gaps = 3/337 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWS---ECND 832
L SL+LG++ +V+AS+P AA E+LKTHDR LSGRYV S VK+ N + + EC +
Sbjct: 1117 LMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTN 1176
Query: 831 TWKKLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFG 652
WK LR +CRTE+F+ K +ES ++RE K ME+V++L EG VK++++VF T+ NI
Sbjct: 1177 NWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILS 1236
Query: 651 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 472
N FS ++ E +K+ ++R ELG + N +D++P L +L G +K + L
Sbjct: 1237 NTFFSMDLCDFEREG-----LKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEAL 1291
Query: 471 QGIYSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 292
I + W LKER+ K + S D +E L+ +D QIN +++E+F AG +TS T
Sbjct: 1292 GRILATWEGTLKERRKQK-NPGSSHRDLLEAFLEIRFEDDQINQVILELFSAGADTSTLT 1350
Query: 291 IEWALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 112
IEWA+++L +NPDV +R EL ++G+ PV+ES +P++PYLQA VKE
Sbjct: 1351 IEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLPYLQACVKETLRLHPPAPLLL 1410
Query: 111 XXXXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
+ETC+V+ Y IPK+ Q+ VN W +GRDPK W D
Sbjct: 1411 PHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDD 1447
Score = 270 bits (691), Expect = 1e-69
Identities = 139/337 (41%), Positives = 209/337 (62%), Gaps = 3/337 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWS---ECND 832
L SL+LG++ +V+AS+ AA E+LKTHDR LSGRYV + V N + + CN
Sbjct: 72 LMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLAMAFAKVCNS 131
Query: 831 TWKKLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFG 652
W+ L+ +CR ELF+ K +ES+ ++RE K ME+V++L+K EG VK++++V+ T+ NI
Sbjct: 132 DWRNLKAICRMELFSGKAMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILS 191
Query: 651 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 472
N FS + V +K+ L ELG +TN D++P LG D+ G RK + +
Sbjct: 192 NKFFSIDFSDFEGRDVRGVLLKDLLNENAELG-ATNILDFYPILGGLDIQGIRKKLKEIF 250
Query: 471 QGIYSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 292
+ I + W +LKER+ ++H +S DF++ LL++G +D QIN ++ME+F AG ETS+ T
Sbjct: 251 RRIPTTWEDILKERRKQRIHGSSH-GDFLDALLETGFEDDQINHVIMELFFAGPETSSLT 309
Query: 291 IEWALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 112
+EWA++EL KN D + +EL ++G+ PV+ES +P++PYLQA VKE
Sbjct: 310 VEWAMAELIKNQDAMHKLCNELTQIIGESPVRESHLPHLPYLQACVKETLRLHPTGPLLL 369
Query: 111 XXXXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
ETC+++ Y IPK+ I VN W +GRDP W D
Sbjct: 370 PHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGTWED 406
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 286 bits (731), Expect = 2e-74
Identities = 146/337 (43%), Positives = 209/337 (62%), Gaps = 3/337 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN--SIVWS-ECND 832
L SL+LG++ +V+AS+P AA E+LKTHDR LSGRY+ S VK N SIV++ +CN
Sbjct: 72 LMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNT 131
Query: 831 TWKKLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFG 652
WK LR +CR ELF+ K +ESQ ++RE K E+V++L EG VK++++VF T+ NI
Sbjct: 132 NWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILS 191
Query: 651 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 472
N FS ++ DE +K+ + + E G + N +DY+P LG D+ G + +
Sbjct: 192 NKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMF 251
Query: 471 QGIYSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 292
+ I + W +LKER+ + N S DF+E LL+ G +D QIN +++E+F AG ETS+ T
Sbjct: 252 ERIPTTWEDILKERRTQR-SNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLT 310
Query: 291 IEWALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 112
+EWA++EL +N D +R EL +VG+ PV+ES +P +PYLQA VKE
Sbjct: 311 VEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRLHPPAPLLL 370
Query: 111 XXXXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
ETC+V+ Y IPK+ QI VN W + RDPK W D
Sbjct: 371 PHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDD 407
>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 285 bits (730), Expect = 3e-74
Identities = 146/337 (43%), Positives = 209/337 (62%), Gaps = 3/337 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN--SIVWS-ECND 832
L SL+LG++ +V+AS+P AA E+LKTHDR LSGRY+ S VK N SIV++ +CN
Sbjct: 72 LMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNT 131
Query: 831 TWKKLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFG 652
WK LR +CR ELF+ K +ESQ ++RE K E+V++L EG VK++++VF T+ NI
Sbjct: 132 NWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILS 191
Query: 651 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 472
N FS ++ DE +K+ + + E G + N +DY+P LG D+ G + +
Sbjct: 192 NKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMF 251
Query: 471 QGIYSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 292
+ I + W +LKER+ + N S DF+E LL+ G +D QIN +++E+F AG ETS+ T
Sbjct: 252 ERIPTTWEDILKERRTQR-SNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLT 310
Query: 291 IEWALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 112
+EWA++EL +N D +R EL +VG+ PV+ES +P +PYLQA VKE
Sbjct: 311 VEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRLHPPAPLLL 370
Query: 111 XXXXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
ETC+V+ Y IPK+ QI VN W + RDPK W D
Sbjct: 371 PHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDD 407
>ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 493
Score = 282 bits (721), Expect = 3e-73
Identities = 142/337 (42%), Positives = 209/337 (62%), Gaps = 3/337 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWS---ECND 832
L SL+LG++ +V+AS+P AA E+LKTHDR LSGRYV S VK+ N + + EC +
Sbjct: 72 LMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTN 131
Query: 831 TWKKLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFG 652
WK LR +CRTE+F+ K +ES ++RE K ME+V++L EG VK++++VF T+ NI
Sbjct: 132 NWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILS 191
Query: 651 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 472
N FS ++ E +K+ ++R ELG + N +D++P L +L G +K + L
Sbjct: 192 NTFFSMDLCDFEREG-----LKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEAL 246
Query: 471 QGIYSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 292
I + W LKER+ K + S D +E L+ +D QIN +++E+F AG +TS T
Sbjct: 247 GRILATWEGTLKERRKQK-NPGSSHRDLLEAFLEIRFEDDQINQVILELFSAGADTSTLT 305
Query: 291 IEWALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 112
IEWA+++L +NPDV +R EL ++G+ PV+ES +P++PYLQA VKE
Sbjct: 306 IEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLPYLQACVKETLRLHPPAPLLL 365
Query: 111 XXXXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
+ETC+V+ Y IPK+ Q+ VN W +GRDPK W D
Sbjct: 366 PHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDD 402
>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547255|gb|EEF48750.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 278 bits (710), Expect = 6e-72
Identities = 144/337 (42%), Positives = 212/337 (62%), Gaps = 3/337 (0%)
Frame = -3
Query: 1002 LFSLKLGSETVVIASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSI--VWS-ECND 832
L SL+LG+ VV+AS+P AA+EILKTHDR+LSGRY+ + +++V + I VW+ C+D
Sbjct: 71 LISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVLDRIALVWNPSCSD 130
Query: 831 TWKKLRKVCRTELFTQKMIESQADIRESKAMEMVKYLKKNEGNEVKIVEVVFGTLVNIFG 652
WK LR +CR+ELF+ K IESQA +RE K EM+ +L +G V I EVVF T N
Sbjct: 131 QWKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEVVFTTAFNTIS 190
Query: 651 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 472
NL+FS+++ D+ + E+K + ++EL N AD++P L + D G ++ + +CL
Sbjct: 191 NLLFSKDLLSFEDQGNAG-ELKTLISTLMELATCPNIADFYPVLTKLDPQGIKRKMKNCL 249
Query: 471 QGIYSVWGAMLKERKIPKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 292
+ ++ VW +KER+ + +++K DF+++ L +G DD QIN LL+E+F AG +T+ +T
Sbjct: 250 ERMFGVWDIYIKERRERHV-KDARKTDFLDVFLSNGFDDHQINWLLLELFSAGADTTTTT 308
Query: 291 IEWALSELTKNPDVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXX 112
+EWA++EL K V +R EL + +GK ++ES IP + YL A VKE
Sbjct: 309 VEWAMAELLKEITVLEKVREELETEIGKDMIRESHIPQLKYLNACVKETLRLHPPVPFLI 368
Query: 111 XXXXLETCKVLNYIIPKECQIMVNAWGIGRDPKRWTD 1
E C+V+NY IPK QI+VN W IGRDP W D
Sbjct: 369 PRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWED 405