BLASTX nr result
ID: Papaver29_contig00005015
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver29_contig00005015
(1313 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353] 704 0.0
sp|Q7XB10.1|4OMT2_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-co... 704 0.0
sp|Q7XB11.1|4OMT1_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-co... 596 e-167
gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 563 e-157
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe... 538 e-150
gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 486 e-134
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc... 485 e-134
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra... 473 e-130
gb|AJD20227.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra... 440 e-120
gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 399 e-108
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica] 389 e-105
ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 388 e-105
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth... 380 e-102
ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 372 e-100
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr... 372 e-100
gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyl... 369 3e-99
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt... 367 9e-99
gb|AKN62836.1| norcoclaurine 6-O-methyltransferase, partial [Pap... 366 2e-98
gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353] 366 2e-98
sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-meth... 366 2e-98
>gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353]
Length = 359
Score = 704 bits (1816), Expect = 0.0
Identities = 349/357 (97%), Positives = 355/357 (99%)
Frame = -3
Query: 1197 MGTLDAKPAAETQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQL 1018
MG+LDAKPAA TQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQL
Sbjct: 1 MGSLDAKPAAATQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQL 60
Query: 1017 ASKLPITNVSSDYLYRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPM 838
A+KLPITNVSSDYLYR+VRYLVHLNI+EQETCNGGVEKVYSLKPVGTLLLRDAERSMVPM
Sbjct: 61 AAKLPITNVSSDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPM 120
Query: 837 ILGMTQKDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRL 658
ILGMTQKDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRL
Sbjct: 121 ILGMTQKDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRL 180
Query: 657 LTKTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKI 478
LTKTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLP I
Sbjct: 181 LTKTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNI 240
Query: 477 EKVPGDMFKSVPSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEES 298
EKVPGDMFKSVPSAQAILLKLILHDWT+EECVNILKKCKEAIPKETGKVIIVDVALEEES
Sbjct: 241 EKVPGDMFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEES 300
Query: 297 NHELTKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
NHELTKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSH+IRPIRAIQSVIEAFP
Sbjct: 301 NHELTKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357
>sp|Q7XB10.1|4OMT2_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase 2; Short=4'-OMT2; Short=Ps4'OMT2;
AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase
gi|33413894|gb|AAP45314.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 2 [Papaver somniferum]
gi|571330882|gb|AHF27397.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine 4'-
O-methyltransferase 2 [synthetic construct]
Length = 357
Score = 704 bits (1816), Expect = 0.0
Identities = 349/357 (97%), Positives = 355/357 (99%)
Frame = -3
Query: 1197 MGTLDAKPAAETQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQL 1018
MG+LDAKPAA TQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQL
Sbjct: 1 MGSLDAKPAAATQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQL 60
Query: 1017 ASKLPITNVSSDYLYRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPM 838
A+KLPITNVSSDYLYR+VRYLVHLNI+EQETCNGGVEKVYSLKPVGTLLLRDAERSMVPM
Sbjct: 61 AAKLPITNVSSDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPM 120
Query: 837 ILGMTQKDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRL 658
ILGMTQKDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRL
Sbjct: 121 ILGMTQKDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRL 180
Query: 657 LTKTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKI 478
LTKTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLP I
Sbjct: 181 LTKTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNI 240
Query: 477 EKVPGDMFKSVPSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEES 298
EKVPGDMFKSVPSAQAILLKLILHDWT+EECVNILKKCKEAIPKETGKVIIVDVALEEES
Sbjct: 241 EKVPGDMFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEES 300
Query: 297 NHELTKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
NHELTKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSH+IRPIRAIQSVIEAFP
Sbjct: 301 NHELTKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357
>sp|Q7XB11.1|4OMT1_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase 1; Short=4'-OMT1; Short=Ps4'OMT1;
AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase
gi|33413892|gb|AAP45313.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 1 [Papaver somniferum]
Length = 354
Score = 596 bits (1536), Expect = e-167
Identities = 288/348 (82%), Positives = 322/348 (92%)
Frame = -3
Query: 1170 AETQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNV 991
AET EV IKDQAQLWNIIYG+ADSLVLRC VEIGIADIIKNN+G+ITL++L SKLP++NV
Sbjct: 7 AETHEVDIKDQAQLWNIIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNV 66
Query: 990 SSDYLYRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDF 811
+SD LYR++RYLVHLNIL Q+TC GV++VYSLKPVGTLLL+D+ERSM P+ILG++QKDF
Sbjct: 67 NSDNLYRLLRYLVHLNILGQQTCAAGVDRVYSLKPVGTLLLKDSERSMAPVILGLSQKDF 126
Query: 810 MVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDC 631
+ W+F+KEGLG GSTTAFEK MGMD+WKYLE NP+QSQLF+EG AGETRLLTKTL+ DC
Sbjct: 127 LFVWNFVKEGLGTGSTTAFEKAMGMDMWKYLEVNPNQSQLFDEGQAGETRLLTKTLLVDC 186
Query: 630 RDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFK 451
RDTFQG+DSLVD+GGGNGTTIKAI+EAFPHIKCTLYDLPHV+ANS D P I KVPGDMF
Sbjct: 187 RDTFQGMDSLVDVGGGNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNILKVPGDMFM 246
Query: 450 SVPSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRL 271
SVPSAQ +LLK +LHDWT+E CVNILKKCKEAIPKETGKVIIVDVALEEES HELTK RL
Sbjct: 247 SVPSAQVLLLKCVLHDWTDEHCVNILKKCKEAIPKETGKVIIVDVALEEESEHELTKARL 306
Query: 270 ILDIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
ILDIDMLVNTGGRERTA+DWENLLKRAGFRSH+IRPIRAIQSVIEAFP
Sbjct: 307 ILDIDMLVNTGGRERTAEDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 354
>gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 353
Score = 563 bits (1450), Expect = e-157
Identities = 280/357 (78%), Positives = 320/357 (89%)
Frame = -3
Query: 1197 MGTLDAKPAAETQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQL 1018
MG D KP E+QEV IK QA LWNIIYGFADSLVLRCAVEIGIADIIK+N+G+I++ +L
Sbjct: 1 MGVSDNKP--ESQEVDIKAQAHLWNIIYGFADSLVLRCAVEIGIADIIKSNNGSISVTEL 58
Query: 1017 ASKLPITNVSSDYLYRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPM 838
ASKLPITNV+SD LYR++RYLVH+ IL++ + + V K+YSL+PV TLLLRDAERSMVP+
Sbjct: 59 ASKLPITNVNSDNLYRVLRYLVHMGILKEVSDSNEV-KLYSLQPVATLLLRDAERSMVPI 117
Query: 837 ILGMTQKDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRL 658
ILGMTQKDFM+ WHFMKEGLGN TTAFEKGMGM IW+YLEG+P+QS LFNEGMAGETRL
Sbjct: 118 ILGMTQKDFMIPWHFMKEGLGN-DTTAFEKGMGMTIWQYLEGHPEQSNLFNEGMAGETRL 176
Query: 657 LTKTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKI 478
LTK+LI+ CRDTF+GL SL D+GGGNGTTIK IY+AFP IKC++YDLPHV+A+S + P I
Sbjct: 177 LTKSLIDGCRDTFEGLTSLCDVGGGNGTTIKGIYDAFPQIKCSVYDLPHVIASSPEHPNI 236
Query: 477 EKVPGDMFKSVPSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEES 298
E++PGDMFKSVPSAQAILLKLILHDWT+EECVNIL KC+EA+PK+TGKVIIVDVALEEES
Sbjct: 237 ERIPGDMFKSVPSAQAILLKLILHDWTDEECVNILIKCREAVPKDTGKVIIVDVALEEES 296
Query: 297 NHELTKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
HELTKTRLILDIDMLVNTGGRER+ DDWE LLKRAGFR H+IR I AIQSVIEAFP
Sbjct: 297 QHELTKTRLILDIDMLVNTGGRERSEDDWEKLLKRAGFRGHKIRHIAAIQSVIEAFP 353
>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
californica]
Length = 353
Score = 538 bits (1387), Expect = e-150
Identities = 259/348 (74%), Positives = 314/348 (90%), Gaps = 3/348 (0%)
Frame = -3
Query: 1161 QEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNVSSD 982
+EV IK QA LWNIIYGFADSLVLR AVE+GIADIIKNN+G+IT+++LASKLPI+NV+SD
Sbjct: 7 EEVDIKAQAHLWNIIYGFADSLVLRSAVELGIADIIKNNNGSITVSELASKLPISNVNSD 66
Query: 981 YLYRIVRYLVHLNILEQ--ETCNGG-VEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDF 811
LYR++RYLVH+ IL++ T NGG ++K+YSL+PVG+LL++DAER+MVP++LGMTQ+DF
Sbjct: 67 NLYRVLRYLVHMGILKETKSTINGGEIKKLYSLEPVGSLLVKDAERNMVPIVLGMTQQDF 126
Query: 810 MVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDC 631
M+ WH++KEGLG GST AFEKGMGM +W+YLEG+P+Q LFN GM GETRLLTKTLIE C
Sbjct: 127 MIPWHYIKEGLGEGST-AFEKGMGMTLWEYLEGHPEQGHLFNVGMEGETRLLTKTLIESC 185
Query: 630 RDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFK 451
+DTF+GL SLVD+GGGNGTTIKAI EAFPHIKC+LYDLPHVVA+SHDLP IEK+PGD+FK
Sbjct: 186 KDTFEGLSSLVDVGGGNGTTIKAISEAFPHIKCSLYDLPHVVADSHDLPNIEKIPGDIFK 245
Query: 450 SVPSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRL 271
+P+AQAILLKLILHDW++E+ V ILKKC+EA+P++TG+VIIVDVALEEES H LTKTRL
Sbjct: 246 FIPNAQAILLKLILHDWSDEDSVKILKKCREAVPQDTGRVIIVDVALEEESEHPLTKTRL 305
Query: 270 ILDIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
+LD+DMLVNTGGRER+ DDW LLK AGFR+H+IR I A+QSVIEAFP
Sbjct: 306 VLDVDMLVNTGGRERSEDDWAKLLKLAGFRTHKIRHIAAVQSVIEAFP 353
>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase [Coptis chinensis]
Length = 350
Score = 486 bits (1251), Expect = e-134
Identities = 240/343 (69%), Positives = 286/343 (83%)
Frame = -3
Query: 1155 VSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNVSSDYL 976
+ IK QA +W IIYGFADSLVLRCAVE+GI DI+ NN+ + LA LASKLP++NV+ D L
Sbjct: 10 LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIVDNNNQPMALADLASKLPVSNVNCDNL 69
Query: 975 YRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWH 796
YRI+RYLV + IL E + G EK Y+L+P+ TLL R+A+RSMVPMILGMTQKDFM WH
Sbjct: 70 YRILRYLVKMEILRVEKSDDG-EKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWH 128
Query: 795 FMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQ 616
MK+GL + T AFEK MGM IW+YLEG+PDQSQLFNEGMAGETRLLT +LI RD FQ
Sbjct: 129 SMKDGLSDNGT-AFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQ 187
Query: 615 GLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSVPSA 436
G+DSLVD+GGGNGTT+KAI +AFPHIKCTL+DLPHV+ANS+D P IE++ GDMFKSVPSA
Sbjct: 188 GIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKSVPSA 247
Query: 435 QAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDID 256
QAILLKLILHDW +E+ + ILK+C+ A+PK+ GKVIIVDVAL+EES+HEL+ TRLILDID
Sbjct: 248 QAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDID 307
Query: 255 MLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
MLVNTGG+ERT + WE ++K AGF +IR I AIQSVIE FP
Sbjct: 308 MLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350
>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
gi|9711916|dbj|BAB08005.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
4'-O-methyltransferase [Coptis japonica]
Length = 350
Score = 485 bits (1249), Expect = e-134
Identities = 239/343 (69%), Positives = 287/343 (83%)
Frame = -3
Query: 1155 VSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNVSSDYL 976
+ IK QA +W IIYGFADSLVLRCAVE+GI DII NN+ + LA LASKLP+++V+ D L
Sbjct: 10 LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNL 69
Query: 975 YRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWH 796
YRI+RYLV + IL E + G +K Y+L+P+ TLL R+A+RSMVPMILGMTQKDFM WH
Sbjct: 70 YRILRYLVKMEILRVEKSDDG-QKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWH 128
Query: 795 FMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQ 616
MK+GL + T AFEK MGM IW+YLEG+PDQSQLFNEGMAGETRLLT +LI RD FQ
Sbjct: 129 SMKDGLSDNGT-AFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQ 187
Query: 615 GLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSVPSA 436
G+DSLVD+GGGNGTT+KAI +AFPHIKCTL+DLPHV+ANS+DLP IE++ GDMFKSVPSA
Sbjct: 188 GIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFKSVPSA 247
Query: 435 QAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDID 256
QAI+LKLILHDW +E+ + ILK+C+ A+PK+ GKVIIVDVAL+EES+HEL+ TRLILDID
Sbjct: 248 QAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDID 307
Query: 255 MLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
MLVNTGG+ERT + WE ++K AGF +IR I AIQSVIE FP
Sbjct: 308 MLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350
>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase [Thalictrum
flavum subsp. glaucum]
Length = 348
Score = 473 bits (1217), Expect = e-130
Identities = 233/343 (67%), Positives = 280/343 (81%)
Frame = -3
Query: 1155 VSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNVSSDYL 976
V IK QA +W IIYGFADSLVLRCAVE+GI DII NN+ I LA LASKLPI+NV+ D L
Sbjct: 11 VDIKAQAHVWKIIYGFADSLVLRCAVELGIFDIIDNNNQPIPLADLASKLPISNVNLDNL 70
Query: 975 YRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWH 796
+RI+RYLV + +L + Y+L+P+ L+LR+ +RSMVPM+LGMTQKDFM WH
Sbjct: 71 FRILRYLVKMELLSY-----AADDKYALEPIAKLVLRNEKRSMVPMVLGMTQKDFMTPWH 125
Query: 795 FMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQ 616
MK+GL + TAFEK MGM IW+YLEG+PDQSQLFNEGMAGETRLLT +LI +D FQ
Sbjct: 126 SMKDGLTDNGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSKDMFQ 185
Query: 615 GLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSVPSA 436
+DSLVD+GGGNGTTIKAI AFPHIKCTL+DLPHV+ANS+D P IE++ GDMFK +PSA
Sbjct: 186 SIDSLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKGMPSA 245
Query: 435 QAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDID 256
QAILLKLILHDW +E+ + ILK+C++A+PK+ GKVIIVDVAL+E+S+HEL+ TRLILDID
Sbjct: 246 QAILLKLILHDWNDEDSIKILKQCRKAVPKDGGKVIIVDVALDEDSDHELSSTRLILDID 305
Query: 255 MLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
MLVNTGG+ERT +DWE L+K AGF +IR I AIQSVIE FP
Sbjct: 306 MLVNTGGKERTKEDWEKLVKCAGFSGCKIRHIAAIQSVIEVFP 348
>gb|AJD20227.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
[Sinopodophyllum hexandrum]
Length = 348
Score = 440 bits (1131), Expect = e-120
Identities = 222/346 (64%), Positives = 273/346 (78%), Gaps = 3/346 (0%)
Frame = -3
Query: 1155 VSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNVSSDYL 976
+ IK QA LW IIYGFADSLVLRCAVE+GI DII NN ITL+ LASKLP+ +V++ +L
Sbjct: 11 LDIKAQAYLWKIIYGFADSLVLRCAVELGIPDIINNNHHPITLSDLASKLPLAHVNTHHL 70
Query: 975 YRIVRYLVHLNI--LEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVS 802
YRI+RYLVH+ + L+ ET YSL+ +GTLLLRDA+R+MVP+ILGMTQKDF+ S
Sbjct: 71 YRIMRYLVHMKLVKLDNET------NQYSLESIGTLLLRDAKRNMVPIILGMTQKDFVTS 124
Query: 801 WHFMKEGLG-NGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRD 625
WH+MK+GL NG TAFEKGMGM IW+YL+G+P+QSQ+F G AGE T + D
Sbjct: 125 WHYMKDGLSYNG--TAFEKGMGMTIWEYLQGHPEQSQJFXXGXAGEXXXXTXSXXXXXGD 182
Query: 624 TFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSV 445
S GGNGTTIK I +AFPHIKCTL+DLP+V+ANS+D P IE+V GDMF+SV
Sbjct: 183 LXXXXTSXXXXXGGNGTTIKGISDAFPHIKCTLFDLPYVIANSYDHPNIERVAGDMFQSV 242
Query: 444 PSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLIL 265
PSAQAILLKLILHDW++E+C+ ILK+CK+A+ KE GKVIIVDVAL+E+S+HEL+ TRLIL
Sbjct: 243 PSAQAILLKLILHDWSDEDCIKILKQCKKAVLKEGGKVIIVDVALDEDSDHELSSTRLIL 302
Query: 264 DIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
DIDMLVNTGG+ERT +DW+ L+K AGF+ IRPI A+QS+IE FP
Sbjct: 303 DIDMLVNTGGKERTKEDWDKLVKSAGFQGFNIRPIAAVQSLIEVFP 348
>gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 348
Score = 399 bits (1024), Expect = e-108
Identities = 183/339 (53%), Positives = 256/339 (75%)
Frame = -3
Query: 1143 DQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNVSSDYLYRIV 964
+QA +W +IYGFA+SLVL+CA+++ IAD I N+ ++L++LASKLP+ V+SD LYR++
Sbjct: 10 NQANIWKLIYGFAESLVLKCAIQLEIADTIHNHGEPMSLSELASKLPVQPVNSDRLYRVM 69
Query: 963 RYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKE 784
RYLVH+ + +E + E YSL P L++ E+SMV IL + KDF+ WH +K+
Sbjct: 70 RYLVHMKLFNKEKTSINGEFKYSLAPPAKFLIKGWEKSMVASILAINDKDFLAPWHHLKD 129
Query: 783 GLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQGLDS 604
GL +G AFEK +G IW Y+ NP+++QLFNE MA +TRL+T L+ DC+ F+G+++
Sbjct: 130 GL-SGDCDAFEKALGKSIWVYMSENPEKNQLFNEAMACDTRLVTSALVNDCQSVFKGINT 188
Query: 603 LVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSVPSAQAIL 424
LVD+GGG GT +KAI +AFPHIKC++YDLPHV+A+S ++P + K+ GDMFK++PSA AIL
Sbjct: 189 LVDVGGGTGTAVKAISKAFPHIKCSIYDLPHVIADSPEIPNVVKIEGDMFKAIPSADAIL 248
Query: 423 LKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLVN 244
+K ILHDW ++EC+ ILKKCKEA+P+E GKVIIVDV L + H +K RL LD+DM++N
Sbjct: 249 MKCILHDWNDDECIQILKKCKEAVPQEGGKVIIVDVVLNMDLTHPYSKIRLTLDLDMMLN 308
Query: 243 TGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
TGG+ERT ++W+ L+ AGF S +I I A+QSVIEAFP
Sbjct: 309 TGGKERTVEEWKKLIDAAGFASFKITEISAVQSVIEAFP 347
>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
Length = 351
Score = 389 bits (1000), Expect = e-105
Identities = 182/338 (53%), Positives = 253/338 (74%)
Frame = -3
Query: 1140 QAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNVSSDYLYRIVR 961
QA++W IYGFA+SL+L+ AVE+ IAD I N+ +TL++LASKLP+ V D LYR++R
Sbjct: 14 QAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDRLYRVMR 73
Query: 960 YLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEG 781
YLVH+ + +++ YSL P L++ E+SMV IL + KDFM WH +KEG
Sbjct: 74 YLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLINDKDFMSPWHHLKEG 133
Query: 780 LGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQGLDSL 601
L N S AF+K +G IW Y+ NPD++++FNEGMA ++RL++ L+ +C + FQG+ +L
Sbjct: 134 LVNNSN-AFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNECGNIFQGIQTL 192
Query: 600 VDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSVPSAQAILL 421
VD+GGG GT +KA+ +AFPHIKC++YDLPHV+++S D+P I K+ G+MFKS+PSA AIL+
Sbjct: 193 VDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGEMFKSIPSADAILM 252
Query: 420 KLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLVNT 241
K ILHDW ++EC+ ILKKCKEA+PKE GKVIIVD+ L+ ES H +K RL LD+DM++NT
Sbjct: 253 KCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDMESKHPYSKVRLTLDLDMMLNT 312
Query: 240 GGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
G+ERT ++W+ L+ AGF S++I I A+QSVIEAFP
Sbjct: 313 EGKERTEEEWKKLIDGAGFASYKITQISALQSVIEAFP 350
>ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 388 bits (997), Expect = e-105
Identities = 181/347 (52%), Positives = 258/347 (74%)
Frame = -3
Query: 1167 ETQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNVS 988
E Q+ A++W +YGFAD LVLRCA+++GIADII +TL++L +++P+ V+
Sbjct: 2 EIQKEGQAAAAKIWKFVYGFADCLVLRCAIDLGIADIIHKQGEPLTLSELGAQIPVQPVN 61
Query: 987 SDYLYRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFM 808
+D+L+R++RYLVH+ I +ET +G E Y L P +++ ++S+V +IL +T KDFM
Sbjct: 62 TDHLHRLMRYLVHMKIFTKETLDG--EARYGLAPPAKFIVKGWDKSIVSIILVVTDKDFM 119
Query: 807 VSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCR 628
WH +K+ L G TAFEK +G IW Y+ +P++++LFNEGMA +T+LL L++DC+
Sbjct: 120 APWHCLKDSLC-GEGTAFEKALGRSIWTYMADHPEKNKLFNEGMACDTKLLISALVQDCK 178
Query: 627 DTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKS 448
D FQG+ SLVD+GGG GT ++AI +AFPH+KCT+YDLPHV+A+S D P+++++ GDMFK
Sbjct: 179 DLFQGIMSLVDVGGGTGTAMRAIAKAFPHLKCTIYDLPHVIADSPDYPEVDRIAGDMFKH 238
Query: 447 VPSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLI 268
+PSA AILLK ILHDW + EC+ ILK+CKE++P+E GKVIIVD+ ++ ES H LTKTRL
Sbjct: 239 IPSADAILLKCILHDWDDGECIEILKRCKESVPREGGKVIIVDIVVDLESKHPLTKTRLS 298
Query: 267 LDIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
LD+DM+V TGG+ERT +W+ LL AGF +I I A+QSVI A+P
Sbjct: 299 LDLDMMVTTGGKERTEAEWKKLLNAAGFPVFKITHISAVQSVIVAYP 345
>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase; Short=6-OMT
gi|9711914|dbj|BAB08004.1|
S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase [Coptis japonica]
Length = 347
Score = 380 bits (975), Expect = e-102
Identities = 173/347 (49%), Positives = 255/347 (73%)
Frame = -3
Query: 1167 ETQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNVS 988
E ++ ++ QA+LWN IYGFA+SLVL+CAV++ +A+II N+ ++TL++L+S+LP V+
Sbjct: 2 EVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVN 61
Query: 987 SDYLYRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFM 808
D LYR++RYLVH+ + + + +G E Y L P L++ ++ MV IL +T KDFM
Sbjct: 62 EDALYRVMRYLVHMKLFTKASIDG--ELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFM 119
Query: 807 VSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCR 628
WH++K+GL S TAFEK +G +IW Y+ +P+++QLFNE MA ++RL+ L+++C
Sbjct: 120 APWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECG 179
Query: 627 DTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKS 448
+ F G+ +LVD+GGG GT ++ I AFPHIKCT+YDLPHV+A+S ++ V GDMFK
Sbjct: 180 NIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKF 239
Query: 447 VPSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLI 268
+P A AI++K ILHDW ++EC+ ILK+CKEA+P + GKVIIVD+ L +S H TK RL
Sbjct: 240 IPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLT 299
Query: 267 LDIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
LD+DM++NTGG+ERT ++W+ L+ AG++ H+I I A+QSVIEA+P
Sbjct: 300 LDLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346
>ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 372 bits (956), Expect = e-100
Identities = 172/339 (50%), Positives = 247/339 (72%)
Frame = -3
Query: 1143 DQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNVSSDYLYRIV 964
D+ ++W Y FAD+LVLRCA+E GIADII +TL +L +++P+ V++D+L+R++
Sbjct: 10 DEVEIWKFGYDFADTLVLRCAIEFGIADIIHKQGEPLTLFELGAQIPVQPVNTDHLHRLM 69
Query: 963 RYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKE 784
RY+VH+ I +ET G E+ Y L P G L++ ++SM IL +T +DF WH +K+
Sbjct: 70 RYMVHMKIFTKETLGG--EEXYGLSPHGKFLVKGWDKSMASAILAITDEDFFAPWHCLKD 127
Query: 783 GLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQGLDS 604
L G TAFEK +G IW Y+ +P++++LFNE MA +T +T LI+DC+D FQG+ S
Sbjct: 128 VLA-GEGTAFEKALGKSIWAYVADHPEKNKLFNEVMACDTSFITSVLIQDCKDVFQGIKS 186
Query: 603 LVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSVPSAQAIL 424
+VD+GGG GT ++ I +AFPH+KCT+YDLPHV+A+S D P+++++ GDMFK +PSA AIL
Sbjct: 187 VVDVGGGTGTAMRDIAKAFPHLKCTIYDLPHVIADSPDYPEVDRIAGDMFKHIPSADAIL 246
Query: 423 LKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLVN 244
LK ILHDW + EC+ ILK+CKE++P+E GKVIIVD+ L+ ES LTK RL LD+DM+V
Sbjct: 247 LKWILHDWDDGECIEILKRCKESVPREGGKVIIVDIVLDPESKDPLTKARLRLDLDMMVY 306
Query: 243 TGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
TGG+ER+ +W+ LL AGF ++I + A+QSVI A+P
Sbjct: 307 TGGKERSEAEWKKLLNAAGFPGYKILHVAAVQSVIMAYP 345
>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
glaucum]
Length = 350
Score = 372 bits (956), Expect = e-100
Identities = 171/344 (49%), Positives = 255/344 (74%), Gaps = 2/344 (0%)
Frame = -3
Query: 1152 SIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITNVSSDYLY 973
++ QA+LWN IYGFADSLVL+ AV++ +A+II N+ +TL++L+ LP V+ D LY
Sbjct: 8 NLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQDALY 67
Query: 972 RIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHF 793
R++RYLVH+ + + + +G E Y L P L++ ++ M+ IL +T KDFM WH+
Sbjct: 68 RVLRYLVHMKLFTKSSIDG--ELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPWHY 125
Query: 792 MKEG-LGNGST-TAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTF 619
+KEG L +GST TAFEK +G +IW Y+ +P+++QLFNEGMA +TRL+ L+++C F
Sbjct: 126 LKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSSMF 185
Query: 618 QGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSVPS 439
G+ ++VD+GGG GT ++ I +AFPHIKCT+YDLPHV+A+S +I + GDMFK +P+
Sbjct: 186 DGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMFKYIPN 245
Query: 438 AQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDI 259
A AI++K ILHDW ++EC+ ILK+CK+A+P++ GKVII+D+ L+ +S H TK RL LD+
Sbjct: 246 ADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKMRLTLDL 305
Query: 258 DMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
DM++NTGG+ERT ++W+ L+ AG++ ++I I A+QSVIEA+P
Sbjct: 306 DMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYP 349
>gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyllum hexandrum]
Length = 350
Score = 369 bits (947), Expect = 3e-99
Identities = 177/348 (50%), Positives = 248/348 (71%), Gaps = 1/348 (0%)
Frame = -3
Query: 1167 ETQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITN-V 991
E Q+ +I QA+LWN IYGFA+SLVL+CAVE+ A+II N+ +TL++LAS+LP+ V
Sbjct: 2 EAQKENISSQAKLWNFIYGFAESLVLKCAVELDFANIIHNHGKPMTLSELASQLPVMQPV 61
Query: 990 SSDYLYRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDF 811
+++ LYR++RYLVH+NI + E Y L L++ + MV ILG+T K F
Sbjct: 62 NTNSLYRVMRYLVHINIFTKTLDENDGETKYGLAAPAKFLVKGWDNCMVGSILGITDKVF 121
Query: 810 MVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDC 631
M W+++K+ L G+ TAFE +G DIW+Y+ NP++++LFN MA +T ++ LI +C
Sbjct: 122 MEPWYYIKDELAPGTGTAFELALGKDIWEYMGENPEKNKLFNAAMACDTSMIMSALISEC 181
Query: 630 RDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFK 451
+D F G+ +LVD+GGG GT + I AFP+IKCT+YDLPHV+A+S P+I +V GDMFK
Sbjct: 182 KDKFNGIRTLVDVGGGTGTAARNIARAFPNIKCTVYDLPHVIADSPVYPEINRVSGDMFK 241
Query: 450 SVPSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRL 271
+P+A AIL+K ILHDW ++EC+ ILK+CKEA+P + GKVII+DV L+ ES H TK RL
Sbjct: 242 CIPNADAILMKCILHDWEDKECIEILKRCKEAVPVDGGKVIIIDVVLDGESEHPYTKVRL 301
Query: 270 ILDIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
D+DM++NT G+ERT + W+ L K AG+R + I I A+QSVIEAFP
Sbjct: 302 NSDLDMMLNTEGKERTKEGWKKLFKAAGYRDYNITQISALQSVIEAFP 349
>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase [Papaver
somniferum]
Length = 346
Score = 367 bits (943), Expect = 9e-99
Identities = 178/346 (51%), Positives = 250/346 (72%), Gaps = 2/346 (0%)
Frame = -3
Query: 1158 EVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITN-VSSD 982
++ ++QA++W IYGFA+SLVL+CAV++ IA+ + NN ++L++LASKLP+ V+ D
Sbjct: 6 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNED 65
Query: 981 YLYRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVS 802
L+RI+RYLVH+ + + + + YSL P LLR E+SMV IL + KDF+
Sbjct: 66 RLFRIMRYLVHMELFKIDATT----QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAP 121
Query: 801 WHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDT 622
WH + +GL G+ AFEK +G IW Y+ NP+++QLFN MA +TRL+T L +C+
Sbjct: 122 WHHLGDGL-TGNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSI 180
Query: 621 FQ-GLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSV 445
F G+ +LVD+GGG GT +KAI +AFP IKCT+YDLPHV+A+S ++P I K+ GDMFKS+
Sbjct: 181 FSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 240
Query: 444 PSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLIL 265
PSA AI +K ILHDW ++EC+ ILK+CKEA+PK GKVIIVDV ++ +S H K RL L
Sbjct: 241 PSADAIFMKCILHDWNDDECIQILKRCKEALPK-VGKVIIVDVVIDMDSTHPYAKIRLTL 299
Query: 264 DIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
D+DM++NTGG+ERT ++W+ L AGF SH++ I A+QSVIEA+P
Sbjct: 300 DLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345
>gb|AKN62836.1| norcoclaurine 6-O-methyltransferase, partial [Papaver
pseudo-orientale]
Length = 343
Score = 366 bits (940), Expect = 2e-98
Identities = 178/346 (51%), Positives = 251/346 (72%), Gaps = 2/346 (0%)
Frame = -3
Query: 1158 EVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITN-VSSD 982
++ ++QA++W IYGFA+SLVL+CAV++ IA+ + NN ++L++LASKLP+ V+ D
Sbjct: 4 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNED 63
Query: 981 YLYRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVS 802
L+RI+RYLVH+ + + + + YSL P LLR E+SMV IL + KDF+
Sbjct: 64 RLFRIMRYLVHMELFKIDATT----QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAP 119
Query: 801 WHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDT 622
WH + +GL G+ AFEK +G IW Y+ NP+++QLFN MA +TRL+T L +C+
Sbjct: 120 WHHLGDGL-TGNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSI 178
Query: 621 FQ-GLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSV 445
F G+ +LVD+GGG GT +K I +AFP IKCT+YDLPHV+A+S ++P I K+ GDMFKS+
Sbjct: 179 FSDGIRTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 238
Query: 444 PSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLIL 265
PSA AI +K ILHDW++EEC+ ILK+CKEA+PK+ GKVIIVDV ++ +S H K RL L
Sbjct: 239 PSADAIFMKCILHDWSDEECIQILKRCKEALPKD-GKVIIVDVVIDMDSTHPYAKIRLTL 297
Query: 264 DIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
D+DM++NTGG+ERT ++W+ L AGF SH++ I A+QSVIEA+P
Sbjct: 298 DLDMMLNTGGKERTKEEWKILFDAAGFASHKVTQISAVQSVIEAYP 343
>gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353]
Length = 348
Score = 366 bits (940), Expect = 2e-98
Identities = 178/346 (51%), Positives = 250/346 (72%), Gaps = 2/346 (0%)
Frame = -3
Query: 1158 EVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITN-VSSD 982
++ ++QA++W IYGFA+SLVL+CAV++ IA+ + NN ++L++LASKLP+ V+ D
Sbjct: 6 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNED 65
Query: 981 YLYRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVS 802
L+RI+RYLVH+ + + + + YSL P LLR E+SMV IL + KDF+
Sbjct: 66 RLFRIMRYLVHMELFKIDATT----QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAP 121
Query: 801 WHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDT 622
WH + +GL G+ AFEK +G IW Y+ NP+++QLFN MA +TRL+T L +C+
Sbjct: 122 WHHLGDGL-TGNCDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKSI 180
Query: 621 FQ-GLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSV 445
F G+ +LVD+GGG GT +KAI +AFP IKCT+YDLPHV+A+S ++P I K+ GDMFKS+
Sbjct: 181 FSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 240
Query: 444 PSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLIL 265
PSA AI +K ILHDW ++EC+ ILK+CKEA+PK GKVIIVDV ++ +S H K RL L
Sbjct: 241 PSADAIFMKCILHDWNDDECIQILKRCKEALPK-GGKVIIVDVVIDMDSTHPYAKIRLTL 299
Query: 264 DIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
D+DM++NTGG+ERT ++W+ L AGF SH++ I A+QSVIEA+P
Sbjct: 300 DLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345
>sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; Short=Ps6OMT
gi|33286374|gb|AAQ01669.1| (R,S)-norcoclaurine
6-O-methyltransferase [Papaver somniferum]
gi|571330878|gb|AHF27395.1| (R,S)-norcoclaurine
6-O-methyltransferase [synthetic construct]
Length = 346
Score = 366 bits (940), Expect = 2e-98
Identities = 178/346 (51%), Positives = 250/346 (72%), Gaps = 2/346 (0%)
Frame = -3
Query: 1158 EVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLASKLPITN-VSSD 982
++ ++QA++W IYGFA+SLVL+CAV++ IA+ + NN ++L++LASKLP+ V+ D
Sbjct: 6 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNED 65
Query: 981 YLYRIVRYLVHLNILEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVS 802
L+RI+RYLVH+ + + + + YSL P LLR E+SMV IL + KDF+
Sbjct: 66 RLFRIMRYLVHMELFKIDATT----QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAP 121
Query: 801 WHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDT 622
WH + +GL G+ AFEK +G IW Y+ NP+++QLFN MA +TRL+T L +C+
Sbjct: 122 WHHLGDGL-TGNCDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKSI 180
Query: 621 FQ-GLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPKIEKVPGDMFKSV 445
F G+ +LVD+GGG GT +KAI +AFP IKCT+YDLPHV+A+S ++P I K+ GDMFKS+
Sbjct: 181 FSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 240
Query: 444 PSAQAILLKLILHDWTNEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLIL 265
PSA AI +K ILHDW ++EC+ ILK+CKEA+PK GKVIIVDV ++ +S H K RL L
Sbjct: 241 PSADAIFMKCILHDWNDDECIQILKRCKEALPK-GGKVIIVDVVIDMDSTHPYAKIRLTL 299
Query: 264 DIDMLVNTGGRERTADDWENLLKRAGFRSHRIRPIRAIQSVIEAFP 127
D+DM++NTGG+ERT ++W+ L AGF SH++ I A+QSVIEA+P
Sbjct: 300 DLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345