BLASTX nr result
ID: Papaver29_contig00003472
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver29_contig00003472
(1543 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353] 613 e-173
sp|Q7XB10.1|4OMT2_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-co... 613 e-173
sp|Q7XB11.1|4OMT1_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-co... 569 e-159
gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 546 e-152
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe... 506 e-140
gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 470 e-129
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra... 466 e-128
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc... 464 e-127
gb|AJD20227.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra... 434 e-118
gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 389 e-105
ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 382 e-103
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica] 381 e-103
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth... 369 5e-99
ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 368 9e-99
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr... 367 2e-98
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap... 366 3e-98
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt... 364 1e-97
gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyl... 364 1e-97
gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353] 363 2e-97
sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-meth... 363 2e-97
>gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353]
Length = 359
Score = 613 bits (1582), Expect = e-173
Identities = 306/355 (86%), Positives = 330/355 (92%), Gaps = 3/355 (0%)
Frame = -2
Query: 1476 SIDVKPAE-TQEVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSS 1300
S+D KPA TQEVSIKDQAQLWN+IYGFADSLV+RCAVE+GI DIIKNN+G ITLAQL++
Sbjct: 3 SLDAKPAAATQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAA 62
Query: 1299 KLPIPNVNSDHLYRIVRYLINLNILQQEICRN--VKVYSLKPVGTLLLRDAERSMVPILL 1126
KLPI NV+SD+LYR+VRYL++LNI++QE C KVYSLKPVGTLLLRDAERSMVP++L
Sbjct: 63 KLPITNVSSDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMIL 122
Query: 1125 GNTQKDFLVPWHFVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLT 946
G TQKDF+V WHF+KEGL NGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGET LLT
Sbjct: 123 GMTQKDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT 182
Query: 945 KTLIEDCRDTFQNLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVK 766
KTLIEDCRDTFQ LDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHV+ANS D PNI K
Sbjct: 183 KTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEK 242
Query: 765 IPGDMFKSVPSTQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEH 586
+PGDMFKSVPS QAILLKLILHDWTDE+CVNIL +CKEAI +ETGKVIIVDVALEEES H
Sbjct: 243 VPGDMFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNH 302
Query: 585 ELTKTRLILDIDMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
ELTKTRLILDIDM+VNTGGRERTADDWENLLKRAGFRSHKIR IRAIQSVIEAFP
Sbjct: 303 ELTKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357
>sp|Q7XB10.1|4OMT2_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase 2; Short=4'-OMT2; Short=Ps4'OMT2;
AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase
gi|33413894|gb|AAP45314.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 2 [Papaver somniferum]
gi|571330882|gb|AHF27397.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine 4'-
O-methyltransferase 2 [synthetic construct]
Length = 357
Score = 613 bits (1582), Expect = e-173
Identities = 306/355 (86%), Positives = 330/355 (92%), Gaps = 3/355 (0%)
Frame = -2
Query: 1476 SIDVKPAE-TQEVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSS 1300
S+D KPA TQEVSIKDQAQLWN+IYGFADSLV+RCAVE+GI DIIKNN+G ITLAQL++
Sbjct: 3 SLDAKPAAATQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAA 62
Query: 1299 KLPIPNVNSDHLYRIVRYLINLNILQQEICRN--VKVYSLKPVGTLLLRDAERSMVPILL 1126
KLPI NV+SD+LYR+VRYL++LNI++QE C KVYSLKPVGTLLLRDAERSMVP++L
Sbjct: 63 KLPITNVSSDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMIL 122
Query: 1125 GNTQKDFLVPWHFVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLT 946
G TQKDF+V WHF+KEGL NGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGET LLT
Sbjct: 123 GMTQKDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT 182
Query: 945 KTLIEDCRDTFQNLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVK 766
KTLIEDCRDTFQ LDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHV+ANS D PNI K
Sbjct: 183 KTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEK 242
Query: 765 IPGDMFKSVPSTQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEH 586
+PGDMFKSVPS QAILLKLILHDWTDE+CVNIL +CKEAI +ETGKVIIVDVALEEES H
Sbjct: 243 VPGDMFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNH 302
Query: 585 ELTKTRLILDIDMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
ELTKTRLILDIDM+VNTGGRERTADDWENLLKRAGFRSHKIR IRAIQSVIEAFP
Sbjct: 303 ELTKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357
>sp|Q7XB11.1|4OMT1_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase 1; Short=4'-OMT1; Short=Ps4'OMT1;
AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase
gi|33413892|gb|AAP45313.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 1 [Papaver somniferum]
Length = 354
Score = 569 bits (1466), Expect = e-159
Identities = 281/355 (79%), Positives = 316/355 (89%), Gaps = 2/355 (0%)
Frame = -2
Query: 1479 SSIDVKPAETQEVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSS 1300
SSID AET EV IKDQAQLWN+IYG+ADSLV+RC VE+GI DIIKNNNG ITL++L S
Sbjct: 3 SSID---AETHEVDIKDQAQLWNIIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVS 59
Query: 1299 KLPIPNVNSDHLYRIVRYLINLNILQQEICRNV--KVYSLKPVGTLLLRDAERSMVPILL 1126
KLP+ NVNSD+LYR++RYL++LNIL Q+ C +VYSLKPVGTLLL+D+ERSM P++L
Sbjct: 60 KLPLSNVNSDNLYRLLRYLVHLNILGQQTCAAGVDRVYSLKPVGTLLLKDSERSMAPVIL 119
Query: 1125 GNTQKDFLVPWHFVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLT 946
G +QKDFL W+FVKEGL GSTTAFEK MGMD+WKYLE NP+QSQLF+EG AGET LLT
Sbjct: 120 GLSQKDFLFVWNFVKEGLGTGSTTAFEKAMGMDMWKYLEVNPNQSQLFDEGQAGETRLLT 179
Query: 945 KTLIEDCRDTFQNLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVK 766
KTL+ DCRDTFQ +DSLVD+GGGNGTTIKAI+EAFPHIKCTLYDLPHVIANS DHPNI+K
Sbjct: 180 KTLLVDCRDTFQGMDSLVDVGGGNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNILK 239
Query: 765 IPGDMFKSVPSTQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEH 586
+PGDMF SVPS Q +LLK +LHDWTDE CVNIL +CKEAI +ETGKVIIVDVALEEESEH
Sbjct: 240 VPGDMFMSVPSAQVLLLKCVLHDWTDEHCVNILKKCKEAIPKETGKVIIVDVALEEESEH 299
Query: 585 ELTKTRLILDIDMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
ELTK RLILDIDM+VNTGGRERTA+DWENLLKRAGFRSHKIR IRAIQSVIEAFP
Sbjct: 300 ELTKARLILDIDMLVNTGGRERTAEDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 354
>gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 353
Score = 546 bits (1406), Expect = e-152
Identities = 268/351 (76%), Positives = 313/351 (89%), Gaps = 1/351 (0%)
Frame = -2
Query: 1470 DVKPAETQEVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLP 1291
D KP E+QEV IK QA LWN+IYGFADSLV+RCAVE+GI DIIK+NNG I++ +L+SKLP
Sbjct: 5 DNKP-ESQEVDIKAQAHLWNIIYGFADSLVLRCAVEIGIADIIKSNNGSISVTELASKLP 63
Query: 1290 IPNVNSDHLYRIVRYLINLNILQQEICRN-VKVYSLKPVGTLLLRDAERSMVPILLGNTQ 1114
I NVNSD+LYR++RYL+++ IL++ N VK+YSL+PV TLLLRDAERSMVPI+LG TQ
Sbjct: 64 ITNVNSDNLYRVLRYLVHMGILKEVSDSNEVKLYSLQPVATLLLRDAERSMVPIILGMTQ 123
Query: 1113 KDFLVPWHFVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLI 934
KDF++PWHF+KEGL N TTAFEKGMGM IW+YLEG+P+QS LFNEGMAGET LLTK+LI
Sbjct: 124 KDFMIPWHFMKEGLGN-DTTAFEKGMGMTIWQYLEGHPEQSNLFNEGMAGETRLLTKSLI 182
Query: 933 EDCRDTFQNLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGD 754
+ CRDTF+ L SL D+GGGNGTTIK IY+AFP IKC++YDLPHVIA+S +HPNI +IPGD
Sbjct: 183 DGCRDTFEGLTSLCDVGGGNGTTIKGIYDAFPQIKCSVYDLPHVIASSPEHPNIERIPGD 242
Query: 753 MFKSVPSTQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTK 574
MFKSVPS QAILLKLILHDWTDE+CVNILI+C+EA+ ++TGKVIIVDVALEEES+HELTK
Sbjct: 243 MFKSVPSAQAILLKLILHDWTDEECVNILIKCREAVPKDTGKVIIVDVALEEESQHELTK 302
Query: 573 TRLILDIDMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
TRLILDIDM+VNTGGRER+ DDWE LLKRAGFR HKIR I AIQSVIEAFP
Sbjct: 303 TRLILDIDMLVNTGGRERSEDDWEKLLKRAGFRGHKIRHIAAIQSVIEAFP 353
>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
californica]
Length = 353
Score = 506 bits (1302), Expect = e-140
Identities = 246/348 (70%), Positives = 298/348 (85%), Gaps = 5/348 (1%)
Frame = -2
Query: 1449 QEVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPNVNSD 1270
+EV IK QA LWN+IYGFADSLV+R AVELGI DIIKNNNG IT+++L+SKLPI NVNSD
Sbjct: 7 EEVDIKAQAHLWNIIYGFADSLVLRSAVELGIADIIKNNNGSITVSELASKLPISNVNSD 66
Query: 1269 HLYRIVRYLINLNILQQEIC-----RNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDF 1105
+LYR++RYL+++ IL++ K+YSL+PVG+LL++DAER+MVPI+LG TQ+DF
Sbjct: 67 NLYRVLRYLVHMGILKETKSTINGGEIKKLYSLEPVGSLLVKDAERNMVPIVLGMTQQDF 126
Query: 1104 LVPWHFVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDC 925
++PWH++KEGL GST AFEKGMGM +W+YLEG+P+Q LFN GM GET LLTKTLIE C
Sbjct: 127 MIPWHYIKEGLGEGST-AFEKGMGMTLWEYLEGHPEQGHLFNVGMEGETRLLTKTLIESC 185
Query: 924 RDTFQNLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFK 745
+DTF+ L SLVD+GGGNGTTIKAI EAFPHIKC+LYDLPHV+A+S D PNI KIPGD+FK
Sbjct: 186 KDTFEGLSSLVDVGGGNGTTIKAISEAFPHIKCSLYDLPHVVADSHDLPNIEKIPGDIFK 245
Query: 744 SVPSTQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRL 565
+P+ QAILLKLILHDW+DE V IL +C+EA+ Q+TG+VIIVDVALEEESEH LTKTRL
Sbjct: 246 FIPNAQAILLKLILHDWSDEDSVKILKKCREAVPQDTGRVIIVDVALEEESEHPLTKTRL 305
Query: 564 ILDIDMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
+LD+DM+VNTGGRER+ DDW LLK AGFR+HKIR I A+QSVIEAFP
Sbjct: 306 VLDVDMLVNTGGRERSEDDWAKLLKLAGFRTHKIRHIAAVQSVIEAFP 353
>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase [Coptis chinensis]
Length = 350
Score = 470 bits (1210), Expect = e-129
Identities = 232/342 (67%), Positives = 280/342 (81%), Gaps = 1/342 (0%)
Frame = -2
Query: 1443 VSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPNVNSDHL 1264
+ IK QA +W +IYGFADSLV+RCAVELGI DI+ NNN P+ LA L+SKLP+ NVN D+L
Sbjct: 10 LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIVDNNNQPMALADLASKLPVSNVNCDNL 69
Query: 1263 YRIVRYLINLNILQQEICRN-VKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWHF 1087
YRI+RYL+ + IL+ E + K Y+L+P+ TLL R+A+RSMVP++LG TQKDF+ PWH
Sbjct: 70 YRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHS 129
Query: 1086 VKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQN 907
+K+GL + T AFEK MGM IW+YLEG+PDQSQLFNEGMAGET LLT +LI RD FQ
Sbjct: 130 MKDGLSDNGT-AFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQG 188
Query: 906 LDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPSTQ 727
+DSLVD+GGGNGTT+KAI +AFPHIKCTL+DLPHVIANS DHPNI +I GDMFKSVPS Q
Sbjct: 189 IDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKSVPSAQ 248
Query: 726 AILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDIDM 547
AILLKLILHDW DE + IL +C+ A+ ++ GKVIIVDVAL+EES+HEL+ TRLILDIDM
Sbjct: 249 AILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDIDM 308
Query: 546 MVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
+VNTGG+ERT + WE ++K AGF KIR I AIQSVIE FP
Sbjct: 309 LVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350
>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase [Thalictrum
flavum subsp. glaucum]
Length = 348
Score = 466 bits (1198), Expect = e-128
Identities = 229/341 (67%), Positives = 276/341 (80%)
Frame = -2
Query: 1443 VSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPNVNSDHL 1264
V IK QA +W +IYGFADSLV+RCAVELGI DII NNN PI LA L+SKLPI NVN D+L
Sbjct: 11 VDIKAQAHVWKIIYGFADSLVLRCAVELGIFDIIDNNNQPIPLADLASKLPISNVNLDNL 70
Query: 1263 YRIVRYLINLNILQQEICRNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWHFV 1084
+RI+RYL+ + +L Y+L+P+ L+LR+ +RSMVP++LG TQKDF+ PWH +
Sbjct: 71 FRILRYLVKMELLSYAADDK---YALEPIAKLVLRNEKRSMVPMVLGMTQKDFMTPWHSM 127
Query: 1083 KEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQNL 904
K+GL + TAFEK MGM IW+YLEG+PDQSQLFNEGMAGET LLT +LI +D FQ++
Sbjct: 128 KDGLTDNGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSKDMFQSI 187
Query: 903 DSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPSTQA 724
DSLVD+GGGNGTTIKAI AFPHIKCTL+DLPHVIANS DHPNI +I GDMFK +PS QA
Sbjct: 188 DSLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKGMPSAQA 247
Query: 723 ILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDIDMM 544
ILLKLILHDW DE + IL +C++A+ ++ GKVIIVDVAL+E+S+HEL+ TRLILDIDM+
Sbjct: 248 ILLKLILHDWNDEDSIKILKQCRKAVPKDGGKVIIVDVALDEDSDHELSSTRLILDIDML 307
Query: 543 VNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
VNTGG+ERT +DWE L+K AGF KIR I AIQSVIE FP
Sbjct: 308 VNTGGKERTKEDWEKLVKCAGFSGCKIRHIAAIQSVIEVFP 348
>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
gi|9711916|dbj|BAB08005.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
4'-O-methyltransferase [Coptis japonica]
Length = 350
Score = 464 bits (1193), Expect = e-127
Identities = 230/342 (67%), Positives = 279/342 (81%), Gaps = 1/342 (0%)
Frame = -2
Query: 1443 VSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPNVNSDHL 1264
+ IK QA +W +IYGFADSLV+RCAVELGI DII NNN P+ LA L+SKLP+ +VN D+L
Sbjct: 10 LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNL 69
Query: 1263 YRIVRYLINLNILQQEICRN-VKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWHF 1087
YRI+RYL+ + IL+ E + K Y+L+P+ TLL R+A+RSMVP++LG TQKDF+ PWH
Sbjct: 70 YRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHS 129
Query: 1086 VKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQN 907
+K+GL + T AFEK MGM IW+YLEG+PDQSQLFNEGMAGET LLT +LI RD FQ
Sbjct: 130 MKDGLSDNGT-AFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQG 188
Query: 906 LDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPSTQ 727
+DSLVD+GGGNGTT+KAI +AFPHIKCTL+DLPHVIANS D PNI +I GDMFKSVPS Q
Sbjct: 189 IDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFKSVPSAQ 248
Query: 726 AILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDIDM 547
AI+LKLILHDW DE + IL +C+ A+ ++ GKVIIVDVAL+EES+HEL+ TRLILDIDM
Sbjct: 249 AIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDIDM 308
Query: 546 MVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
+VNTGG+ERT + WE ++K AGF KIR I AIQSVIE FP
Sbjct: 309 LVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350
>gb|AJD20227.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
[Sinopodophyllum hexandrum]
Length = 348
Score = 434 bits (1115), Expect = e-118
Identities = 213/342 (62%), Positives = 269/342 (78%), Gaps = 1/342 (0%)
Frame = -2
Query: 1443 VSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPNVNSDHL 1264
+ IK QA LW +IYGFADSLV+RCAVELGIPDII NN+ PITL+ L+SKLP+ +VN+ HL
Sbjct: 11 LDIKAQAYLWKIIYGFADSLVLRCAVELGIPDIINNNHHPITLSDLASKLPLAHVNTHHL 70
Query: 1263 YRIVRYLINLNILQQEICRNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWHFV 1084
YRI+RYL+++ +++ + YSL+ +GTLLLRDA+R+MVPI+LG TQKDF+ WH++
Sbjct: 71 YRIMRYLVHMKLVKLD--NETNQYSLESIGTLLLRDAKRNMVPIILGMTQKDFVTSWHYM 128
Query: 1083 KEGL-VNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQN 907
K+GL NG TAFEKGMGM IW+YL+G+P+QSQ+F G AGE T + D
Sbjct: 129 KDGLSYNG--TAFEKGMGMTIWEYLQGHPEQSQJFXXGXAGEXXXXTXSXXXXXGDLXXX 186
Query: 906 LDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPSTQ 727
S GGNGTTIK I +AFPHIKCTL+DLP+VIANS DHPNI ++ GDMF+SVPS Q
Sbjct: 187 XTSXXXXXGGNGTTIKGISDAFPHIKCTLFDLPYVIANSYDHPNIERVAGDMFQSVPSAQ 246
Query: 726 AILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDIDM 547
AILLKLILHDW+DE C+ IL +CK+A+ +E GKVIIVDVAL+E+S+HEL+ TRLILDIDM
Sbjct: 247 AILLKLILHDWSDEDCIKILKQCKKAVLKEGGKVIIVDVALDEDSDHELSSTRLILDIDM 306
Query: 546 MVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
+VNTGG+ERT +DW+ L+K AGF+ IR I A+QS+IE FP
Sbjct: 307 LVNTGGKERTKEDWDKLVKSAGFQGFNIRPIAAVQSLIEVFP 348
>gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 348
Score = 389 bits (998), Expect = e-105
Identities = 184/340 (54%), Positives = 253/340 (74%), Gaps = 3/340 (0%)
Frame = -2
Query: 1431 DQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPNVNSDHLYRIV 1252
+QA +W +IYGFA+SLV++CA++L I D I N+ P++L++L+SKLP+ VNSD LYR++
Sbjct: 10 NQANIWKLIYGFAESLVLKCAIQLEIADTIHNHGEPMSLSELASKLPVQPVNSDRLYRVM 69
Query: 1251 RYLINLNILQQE---ICRNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWHFVK 1081
RYL+++ + +E I K YSL P L++ E+SMV +L KDFL PWH +K
Sbjct: 70 RYLVHMKLFNKEKTSINGEFK-YSLAPPAKFLIKGWEKSMVASILAINDKDFLAPWHHLK 128
Query: 1080 EGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQNLD 901
+GL +G AFEK +G IW Y+ NP+++QLFNE MA +T L+T L+ DC+ F+ ++
Sbjct: 129 DGL-SGDCDAFEKALGKSIWVYMSENPEKNQLFNEAMACDTRLVTSALVNDCQSVFKGIN 187
Query: 900 SLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPSTQAI 721
+LVD+GGG GT +KAI +AFPHIKC++YDLPHVIA+S + PN+VKI GDMFK++PS AI
Sbjct: 188 TLVDVGGGTGTAVKAISKAFPHIKCSIYDLPHVIADSPEIPNVVKIEGDMFKAIPSADAI 247
Query: 720 LLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDIDMMV 541
L+K ILHDW D++C+ IL +CKEA+ QE GKVIIVDV L + H +K RL LD+DMM+
Sbjct: 248 LMKCILHDWNDDECIQILKKCKEAVPQEGGKVIIVDVVLNMDLTHPYSKIRLTLDLDMML 307
Query: 540 NTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
NTGG+ERT ++W+ L+ AGF S KI +I A+QSVIEAFP
Sbjct: 308 NTGGKERTVEEWKKLIDAAGFASFKITEISAVQSVIEAFP 347
>ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 382 bits (981), Expect = e-103
Identities = 179/345 (51%), Positives = 249/345 (72%)
Frame = -2
Query: 1455 ETQEVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPNVN 1276
E Q+ A++W +YGFAD LV+RCA++LGI DII P+TL++L +++P+ VN
Sbjct: 2 EIQKEGQAAAAKIWKFVYGFADCLVLRCAIDLGIADIIHKQGEPLTLSELGAQIPVQPVN 61
Query: 1275 SDHLYRIVRYLINLNILQQEICRNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVP 1096
+DHL+R++RYL+++ I +E Y L P +++ ++S+V I+L T KDF+ P
Sbjct: 62 TDHLHRLMRYLVHMKIFTKETLDGEARYGLAPPAKFIVKGWDKSIVSIILVVTDKDFMAP 121
Query: 1095 WHFVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDT 916
WH +K+ L G TAFEK +G IW Y+ +P++++LFNEGMA +T LL L++DC+D
Sbjct: 122 WHCLKDSLC-GEGTAFEKALGRSIWTYMADHPEKNKLFNEGMACDTKLLISALVQDCKDL 180
Query: 915 FQNLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVP 736
FQ + SLVD+GGG GT ++AI +AFPH+KCT+YDLPHVIA+S D+P + +I GDMFK +P
Sbjct: 181 FQGIMSLVDVGGGTGTAMRAIAKAFPHLKCTIYDLPHVIADSPDYPEVDRIAGDMFKHIP 240
Query: 735 STQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILD 556
S AILLK ILHDW D +C+ IL RCKE++ +E GKVIIVD+ ++ ES+H LTKTRL LD
Sbjct: 241 SADAILLKCILHDWDDGECIEILKRCKESVPREGGKVIIVDIVVDLESKHPLTKTRLSLD 300
Query: 555 IDMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
+DMMV TGG+ERT +W+ LL AGF KI I A+QSVI A+P
Sbjct: 301 LDMMVTTGGKERTEAEWKKLLNAAGFPVFKITHISAVQSVIVAYP 345
>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
Length = 351
Score = 381 bits (979), Expect = e-103
Identities = 179/338 (52%), Positives = 255/338 (75%), Gaps = 2/338 (0%)
Frame = -2
Query: 1428 QAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPNVNSDHLYRIVR 1249
QA++W IYGFA+SL+++ AVEL I D I N+ P+TL++L+SKLP+ V+ D LYR++R
Sbjct: 14 QAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDRLYRVMR 73
Query: 1248 YLINLNILQQEICRNVKV--YSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWHFVKEG 1075
YL+++ + +++ + + YSL P L++ E+SMV +L KDF+ PWH +KEG
Sbjct: 74 YLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLINDKDFMSPWHHLKEG 133
Query: 1074 LVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQNLDSL 895
LVN S AF+K +G IW Y+ NPD++++FNEGMA ++ L++ L+ +C + FQ + +L
Sbjct: 134 LVNNSN-AFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNECGNIFQGIQTL 192
Query: 894 VDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPSTQAILL 715
VD+GGG GT +KA+ +AFPHIKC++YDLPHVI++S D PNIVKI G+MFKS+PS AIL+
Sbjct: 193 VDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGEMFKSIPSADAILM 252
Query: 714 KLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDIDMMVNT 535
K ILHDW D++C+ IL +CKEA+ +E GKVIIVD+ L+ ES+H +K RL LD+DMM+NT
Sbjct: 253 KCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDMESKHPYSKVRLTLDLDMMLNT 312
Query: 534 GGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
G+ERT ++W+ L+ AGF S+KI QI A+QSVIEAFP
Sbjct: 313 EGKERTEEEWKKLIDGAGFASYKITQISALQSVIEAFP 350
>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase; Short=6-OMT
gi|9711914|dbj|BAB08004.1|
S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase [Coptis japonica]
Length = 347
Score = 369 bits (946), Expect = 5e-99
Identities = 169/345 (48%), Positives = 246/345 (71%)
Frame = -2
Query: 1455 ETQEVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPNVN 1276
E ++ ++ QA+LWN IYGFA+SLV++CAV+L + +II N+ +TL++LSS+LP VN
Sbjct: 2 EVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVN 61
Query: 1275 SDHLYRIVRYLINLNILQQEICRNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVP 1096
D LYR++RYL+++ + + Y L P L++ ++ MV +L T KDF+ P
Sbjct: 62 EDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAP 121
Query: 1095 WHFVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDT 916
WH++K+GL S TAFEK +G +IW Y+ +P+++QLFNE MA ++ L+ L+++C +
Sbjct: 122 WHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNI 181
Query: 915 FQNLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVP 736
F + +LVD+GGG GT ++ I AFPHIKCT+YDLPHVIA+S + + + GDMFK +P
Sbjct: 182 FNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIP 241
Query: 735 STQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILD 556
AI++K ILHDW D++C+ IL RCKEA+ + GKVIIVD+ L +SEH TK RL LD
Sbjct: 242 KADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLD 301
Query: 555 IDMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
+DMM+NTGG+ERT ++W+ L+ AG++ HKI QI A+QSVIEA+P
Sbjct: 302 LDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346
>ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 368 bits (944), Expect = 9e-99
Identities = 170/337 (50%), Positives = 241/337 (71%)
Frame = -2
Query: 1431 DQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPNVNSDHLYRIV 1252
D+ ++W Y FAD+LV+RCA+E GI DII P+TL +L +++P+ VN+DHL+R++
Sbjct: 10 DEVEIWKFGYDFADTLVLRCAIEFGIADIIHKQGEPLTLFELGAQIPVQPVNTDHLHRLM 69
Query: 1251 RYLINLNILQQEICRNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWHFVKEGL 1072
RY++++ I +E + Y L P G L++ ++SM +L T +DF PWH +K+ L
Sbjct: 70 RYMVHMKIFTKETLGGEEXYGLSPHGKFLVKGWDKSMASAILAITDEDFFAPWHCLKDVL 129
Query: 1071 VNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQNLDSLV 892
G TAFEK +G IW Y+ +P++++LFNE MA +TS +T LI+DC+D FQ + S+V
Sbjct: 130 A-GEGTAFEKALGKSIWAYVADHPEKNKLFNEVMACDTSFITSVLIQDCKDVFQGIKSVV 188
Query: 891 DIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPSTQAILLK 712
D+GGG GT ++ I +AFPH+KCT+YDLPHVIA+S D+P + +I GDMFK +PS AILLK
Sbjct: 189 DVGGGTGTAMRDIAKAFPHLKCTIYDLPHVIADSPDYPEVDRIAGDMFKHIPSADAILLK 248
Query: 711 LILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDIDMMVNTG 532
ILHDW D +C+ IL RCKE++ +E GKVIIVD+ L+ ES+ LTK RL LD+DMMV TG
Sbjct: 249 WILHDWDDGECIEILKRCKESVPREGGKVIIVDIVLDPESKDPLTKARLRLDLDMMVYTG 308
Query: 531 GRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
G+ER+ +W+ LL AGF +KI + A+QSVI A+P
Sbjct: 309 GKERSEAEWKKLLNAAGFPGYKILHVAAVQSVIMAYP 345
>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
glaucum]
Length = 350
Score = 367 bits (941), Expect = 2e-98
Identities = 169/342 (49%), Positives = 248/342 (72%), Gaps = 2/342 (0%)
Frame = -2
Query: 1440 SIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPNVNSDHLY 1261
++ QA+LWN IYGFADSLV++ AV+L + +II N+ P+TL++LS LP VN D LY
Sbjct: 8 NLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQDALY 67
Query: 1260 RIVRYLINLNILQQEICRNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWHFVK 1081
R++RYL+++ + + Y L P L++ ++ M+ +L T KDF+ PWH++K
Sbjct: 68 RVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPWHYLK 127
Query: 1080 EGLVN-GST-TAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQN 907
EG++N GST TAFEK +G +IW Y+ +P+++QLFNEGMA +T L+ L+++C F
Sbjct: 128 EGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSSMFDG 187
Query: 906 LDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPSTQ 727
+ ++VD+GGG GT ++ I +AFPHIKCT+YDLPHVIA+S + I I GDMFK +P+
Sbjct: 188 ITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMFKYIPNAD 247
Query: 726 AILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDIDM 547
AI++K ILHDW D++C+ IL RCK+A+ ++ GKVII+D+ L+ +SEH TK RL LD+DM
Sbjct: 248 AIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKMRLTLDLDM 307
Query: 546 MVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
M+NTGG+ERT ++W+ L+ AG++ +KI I A+QSVIEA+P
Sbjct: 308 MLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYP 349
>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
Length = 346
Score = 366 bits (939), Expect = 3e-98
Identities = 180/344 (52%), Positives = 248/344 (72%), Gaps = 2/344 (0%)
Frame = -2
Query: 1446 EVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPN-VNSD 1270
++ ++QA++W IYGFA+SLV++CAV+L I + + NN P++L++L+SKLP+P VN D
Sbjct: 6 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVPQPVNED 65
Query: 1269 HLYRIVRYLINLNILQQEICRNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWH 1090
L+RI+RYL+++ + + I + YSL P LLR E+SMV +L KDFL PWH
Sbjct: 66 RLFRIMRYLVHMELFK--IDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWH 123
Query: 1089 FVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQ 910
+ +GL G+ AFEK +G IW Y+ NP+++QLFN MA +T L+T L +C+ F
Sbjct: 124 HLGDGLT-GNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSIFS 182
Query: 909 N-LDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPS 733
+ + +LVD+GGG GT +K I +AFP IKCT+YDLPHVIA+S + PNI KI GDMFKS+PS
Sbjct: 183 DGIKTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSIPS 242
Query: 732 TQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDI 553
AI +K ILHDW DE+C+ IL RCKEA+ ++ GKVIIVDV ++ +S H K RL LD+
Sbjct: 243 ADAIFMKCILHDWNDEECIQILKRCKEALPKD-GKVIIVDVVIDMDSTHPYAKIRLTLDL 301
Query: 552 DMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
DMM+NTGG+ERT ++W+ L AGF SHK+ QI A+QSVIEA+P
Sbjct: 302 DMMLNTGGKERTKEEWKILFDAAGFASHKVTQISAVQSVIEAYP 345
>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase [Papaver
somniferum]
Length = 346
Score = 364 bits (935), Expect = 1e-97
Identities = 179/344 (52%), Positives = 247/344 (71%), Gaps = 2/344 (0%)
Frame = -2
Query: 1446 EVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPN-VNSD 1270
++ ++QA++W IYGFA+SLV++CAV+L I + + NN P++L++L+SKLP+ VN D
Sbjct: 6 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNED 65
Query: 1269 HLYRIVRYLINLNILQQEICRNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWH 1090
L+RI+RYL+++ + + I + YSL P LLR E+SMV +L KDFL PWH
Sbjct: 66 RLFRIMRYLVHMELFK--IDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWH 123
Query: 1089 FVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQ 910
+ +GL G+ AFEK +G IW Y+ NP+++QLFN MA +T L+T L +C+ F
Sbjct: 124 HLGDGLT-GNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSIFS 182
Query: 909 N-LDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPS 733
+ + +LVD+GGG GT +KAI +AFP IKCT+YDLPHVIA+S + PNI KI GDMFKS+PS
Sbjct: 183 DGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSIPS 242
Query: 732 TQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDI 553
AI +K ILHDW D++C+ IL RCKEA+ + GKVIIVDV ++ +S H K RL LD+
Sbjct: 243 ADAIFMKCILHDWNDDECIQILKRCKEAL-PKVGKVIIVDVVIDMDSTHPYAKIRLTLDL 301
Query: 552 DMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
DMM+NTGG+ERT ++W+ L AGF SHK+ QI A+QSVIEA+P
Sbjct: 302 DMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345
>gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyllum hexandrum]
Length = 350
Score = 364 bits (934), Expect = 1e-97
Identities = 175/348 (50%), Positives = 248/348 (71%), Gaps = 3/348 (0%)
Frame = -2
Query: 1455 ETQEVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPN-V 1279
E Q+ +I QA+LWN IYGFA+SLV++CAVEL +II N+ P+TL++L+S+LP+ V
Sbjct: 2 EAQKENISSQAKLWNFIYGFAESLVLKCAVELDFANIIHNHGKPMTLSELASQLPVMQPV 61
Query: 1278 NSDHLYRIVRYLINLNILQQEICRN--VKVYSLKPVGTLLLRDAERSMVPILLGNTQKDF 1105
N++ LYR++RYL+++NI + + N Y L L++ + MV +LG T K F
Sbjct: 62 NTNSLYRVMRYLVHINIFTKTLDENDGETKYGLAAPAKFLVKGWDNCMVGSILGITDKVF 121
Query: 1104 LVPWHFVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDC 925
+ PW+++K+ L G+ TAFE +G DIW+Y+ NP++++LFN MA +TS++ LI +C
Sbjct: 122 MEPWYYIKDELAPGTGTAFELALGKDIWEYMGENPEKNKLFNAAMACDTSMIMSALISEC 181
Query: 924 RDTFQNLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFK 745
+D F + +LVD+GGG GT + I AFP+IKCT+YDLPHVIA+S +P I ++ GDMFK
Sbjct: 182 KDKFNGIRTLVDVGGGTGTAARNIARAFPNIKCTVYDLPHVIADSPVYPEINRVSGDMFK 241
Query: 744 SVPSTQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRL 565
+P+ AIL+K ILHDW D++C+ IL RCKEA+ + GKVII+DV L+ ESEH TK RL
Sbjct: 242 CIPNADAILMKCILHDWEDKECIEILKRCKEAVPVDGGKVIIIDVVLDGESEHPYTKVRL 301
Query: 564 ILDIDMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
D+DMM+NT G+ERT + W+ L K AG+R + I QI A+QSVIEAFP
Sbjct: 302 NSDLDMMLNTEGKERTKEGWKKLFKAAGYRDYNITQISALQSVIEAFP 349
>gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353]
Length = 348
Score = 363 bits (932), Expect = 2e-97
Identities = 179/344 (52%), Positives = 247/344 (71%), Gaps = 2/344 (0%)
Frame = -2
Query: 1446 EVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPN-VNSD 1270
++ ++QA++W IYGFA+SLV++CAV+L I + + NN P++L++L+SKLP+ VN D
Sbjct: 6 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNED 65
Query: 1269 HLYRIVRYLINLNILQQEICRNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWH 1090
L+RI+RYL+++ + + I + YSL P LLR E+SMV +L KDFL PWH
Sbjct: 66 RLFRIMRYLVHMELFK--IDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWH 123
Query: 1089 FVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQ 910
+ +GL G+ AFEK +G IW Y+ NP+++QLFN MA +T L+T L +C+ F
Sbjct: 124 HLGDGLT-GNCDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKSIFS 182
Query: 909 N-LDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPS 733
+ + +LVD+GGG GT +KAI +AFP IKCT+YDLPHVIA+S + PNI KI GDMFKS+PS
Sbjct: 183 DGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSIPS 242
Query: 732 TQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDI 553
AI +K ILHDW D++C+ IL RCKEA+ + GKVIIVDV ++ +S H K RL LD+
Sbjct: 243 ADAIFMKCILHDWNDDECIQILKRCKEAL-PKGGKVIIVDVVIDMDSTHPYAKIRLTLDL 301
Query: 552 DMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
DMM+NTGG+ERT ++W+ L AGF SHK+ QI A+QSVIEA+P
Sbjct: 302 DMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345
>sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; Short=Ps6OMT
gi|33286374|gb|AAQ01669.1| (R,S)-norcoclaurine
6-O-methyltransferase [Papaver somniferum]
gi|571330878|gb|AHF27395.1| (R,S)-norcoclaurine
6-O-methyltransferase [synthetic construct]
Length = 346
Score = 363 bits (932), Expect = 2e-97
Identities = 179/344 (52%), Positives = 247/344 (71%), Gaps = 2/344 (0%)
Frame = -2
Query: 1446 EVSIKDQAQLWNMIYGFADSLVVRCAVELGIPDIIKNNNGPITLAQLSSKLPIPN-VNSD 1270
++ ++QA++W IYGFA+SLV++CAV+L I + + NN P++L++L+SKLP+ VN D
Sbjct: 6 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNED 65
Query: 1269 HLYRIVRYLINLNILQQEICRNVKVYSLKPVGTLLLRDAERSMVPILLGNTQKDFLVPWH 1090
L+RI+RYL+++ + + I + YSL P LLR E+SMV +L KDFL PWH
Sbjct: 66 RLFRIMRYLVHMELFK--IDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWH 123
Query: 1089 FVKEGLVNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETSLLTKTLIEDCRDTFQ 910
+ +GL G+ AFEK +G IW Y+ NP+++QLFN MA +T L+T L +C+ F
Sbjct: 124 HLGDGLT-GNCDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKSIFS 182
Query: 909 N-LDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVIANSTDHPNIVKIPGDMFKSVPS 733
+ + +LVD+GGG GT +KAI +AFP IKCT+YDLPHVIA+S + PNI KI GDMFKS+PS
Sbjct: 183 DGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSIPS 242
Query: 732 TQAILLKLILHDWTDEQCVNILIRCKEAINQETGKVIIVDVALEEESEHELTKTRLILDI 553
AI +K ILHDW D++C+ IL RCKEA+ + GKVIIVDV ++ +S H K RL LD+
Sbjct: 243 ADAIFMKCILHDWNDDECIQILKRCKEAL-PKGGKVIIVDVVIDMDSTHPYAKIRLTLDL 301
Query: 552 DMMVNTGGRERTADDWENLLKRAGFRSHKIRQIRAIQSVIEAFP 421
DMM+NTGG+ERT ++W+ L AGF SHK+ QI A+QSVIEA+P
Sbjct: 302 DMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345