BLASTX nr result
ID: Papaver29_contig00000875
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver29_contig00000875
(805 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AKO60155.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 402 e-109
gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum] 261 5e-67
gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum] 260 7e-67
ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 214 4e-53
gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 207 5e-51
ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 207 9e-51
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap... 207 9e-51
gb|AKN62836.1| norcoclaurine 6-O-methyltransferase, partial [Pap... 206 2e-50
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr... 204 6e-50
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt... 201 5e-49
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica] 200 1e-48
gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353] 199 1e-48
sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-meth... 199 1e-48
gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyl... 199 2e-48
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth... 198 4e-48
gb|AFK40900.1| unknown [Lotus japonicus] 197 6e-48
ref|XP_012088195.1| PREDICTED: trans-resveratrol di-O-methyltran... 196 2e-47
gb|KHG11439.1| RS-norcoclaurine 6-O-methyltransferase [Gossypium... 196 2e-47
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra... 195 4e-47
gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 194 6e-47
>gb|AKO60155.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 356
Score = 402 bits (1033), Expect = e-109
Identities = 194/266 (72%), Positives = 215/266 (80%)
Frame = +3
Query: 3 KAENLYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSIN 182
K +NLYR+LRYL+HMNLI +N+ E E+FSLT ++KLLLK QERSLKDWVLGIDDEHS+N
Sbjct: 68 KTQNLYRLLRYLTHMNLITINSTEATESFSLTDLAKLLLKTQERSLKDWVLGIDDEHSVN 127
Query: 183 GWHELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGC 362
GWHEL+ YC S DDAPTPFVKLHGKT+WELAEEVPEVN LINN MACDTRMVMPAFVQGC
Sbjct: 128 GWHELSSYCCSPDDAPTPFVKLHGKTLWELAEEVPEVNELINNGMACDTRMVMPAFVQGC 187
Query: 363 DIILKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFE 542
+ +L GI+KLVDIGGGTGAA+SY+VKAFP+IK DL GGDMFE
Sbjct: 188 EKVLSGIRKLVDIGGGTGAAISYVVKAFPQIKGTVLDLPHVVATAPEIPGVEMVGGDMFE 247
Query: 543 SIPPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQAR 722
SIP ADALLLKFMLHNWKD EC+KLLKRCKE IP + GKVIIIEMVLDQDE D+DLTQAR
Sbjct: 248 SIPHADALLLKFMLHNWKDEECIKLLKRCKEAIPTDQGKVIIIEMVLDQDEDDEDLTQAR 307
Query: 723 XXXXXXXXXXXGGKERTKDEWRILLE 800
GGKERTKDEWR+LLE
Sbjct: 308 LSLDLDMMLSSGGKERTKDEWRVLLE 333
>gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum]
Length = 356
Score = 261 bits (666), Expect = 5e-67
Identities = 133/263 (50%), Positives = 172/263 (65%)
Frame = +3
Query: 12 NLYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGWH 191
NLYR++RYLS M+LI++ + T SLTG SKLLL+NQE+SL DWVL I E + WH
Sbjct: 72 NLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLAISCEMMVVVWH 131
Query: 192 ELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDII 371
EL+ + D P F K+HGK ELA E PE N LINNAM DT + PA +QGC I
Sbjct: 132 ELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVTKPALIQGCGKI 191
Query: 372 LKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFESIP 551
L G+ L+D+GGG GA M+YIV+AFP IK DL GD+F+SI
Sbjct: 192 LNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERPGVEFISGDIFKSIS 251
Query: 552 PADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARXXX 731
ADA+LLK++LHNW+D EC+ LLKRCKE +PA+ GKVII+++V+D D+ + LTQA+
Sbjct: 252 NADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVIDDDD-NSILTQAKLSL 310
Query: 732 XXXXXXXXGGKERTKDEWRILLE 800
GG+ERTK++WR L+E
Sbjct: 311 DLTVMNHGGGRERTKEDWRNLIE 333
>gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum]
Length = 356
Score = 260 bits (665), Expect = 7e-67
Identities = 133/263 (50%), Positives = 172/263 (65%)
Frame = +3
Query: 12 NLYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGWH 191
NLYR++RYLS M+LI++ + T SLTG SKLLL+NQE+SL DWVL I E + WH
Sbjct: 72 NLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLAIXCEMMVVVWH 131
Query: 192 ELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDII 371
EL+ + D P F K+HGK ELA E PE N LINNAM DT + PA +QGC I
Sbjct: 132 ELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVTKPALIQGCGKI 191
Query: 372 LKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFESIP 551
L G+ L+D+GGG GA M+YIV+AFP IK DL GD+F+SI
Sbjct: 192 LNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERPGVEFISGDIFKSIS 251
Query: 552 PADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARXXX 731
ADA+LLK++LHNW+D EC+ LLKRCKE +PA+ GKVII+++V+D D+ + LTQA+
Sbjct: 252 NADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVIDDDD-NSILTQAKLSL 310
Query: 732 XXXXXXXXGGKERTKDEWRILLE 800
GG+ERTK++WR L+E
Sbjct: 311 DLTVMNHGGGRERTKEDWRNLIE 333
>ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 214 bits (546), Expect = 4e-53
Identities = 109/263 (41%), Positives = 151/263 (57%)
Frame = +3
Query: 9 ENLYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGW 188
++L+R++RY+ HM + G+E + L+ K L+K ++S+ +L I DE W
Sbjct: 63 DHLHRLMRYMVHMKIFTKETLGGEEXYGLSPHGKFLVKGWDKSMASAILAITDEDFFAPW 122
Query: 189 HELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDI 368
H L D T F K GK+IW + PE N L N MACDT + +Q C
Sbjct: 123 HCLKDVLAGEG---TAFEKALGKSIWAYVADHPEKNKLFNEVMACDTSFITSVLIQDCKD 179
Query: 369 ILKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFESI 548
+ +GIK +VD+GGGTG AM I KAFP +KC +DL GDMF+ I
Sbjct: 180 VFQGIKSVVDVGGGTGTAMRDIAKAFPHLKCTIYDLPHVIADSPDYPEVDRIAGDMFKHI 239
Query: 549 PPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARXX 728
P ADA+LLK++LH+W D EC+++LKRCKE++P GKVII+++VLD E D LT+AR
Sbjct: 240 PSADAILLKWILHDWDDGECIEILKRCKESVPREGGKVIIVDIVLD-PESKDPLTKARLR 298
Query: 729 XXXXXXXXXGGKERTKDEWRILL 797
GGKER++ EW+ LL
Sbjct: 299 LDLDMMVYTGGKERSEAEWKKLL 321
>gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 348
Score = 207 bits (528), Expect = 5e-51
Identities = 112/267 (41%), Positives = 154/267 (57%), Gaps = 2/267 (0%)
Frame = +3
Query: 6 AENLYRMLRYLSHMNLI--AVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSI 179
++ LYR++RYL HM L + G+ +SL +K L+K E+S+ +L I+D+ +
Sbjct: 62 SDRLYRVMRYLVHMKLFNKEKTSINGEFKYSLAPPAKFLIKGWEKSMVASILAINDKDFL 121
Query: 180 NGWHELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQG 359
WH L D DA F K GK+IW E PE N L N AMACDTR+V A V
Sbjct: 122 APWHHLKDGLSGDCDA---FEKALGKSIWVYMSENPEKNQLFNEAMACDTRLVTSALVND 178
Query: 360 CDIILKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMF 539
C + KGI LVD+GGGTG A+ I KAFP IKC +DL GDMF
Sbjct: 179 CQSVFKGINTLVDVGGGTGTAVKAISKAFPHIKCSIYDLPHVIADSPEIPNVVKIEGDMF 238
Query: 540 ESIPPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQA 719
++IP ADA+L+K +LH+W D EC+++LK+CKE +P GKVII+++VL+ D ++
Sbjct: 239 KAIPSADAILMKCILHDWNDDECIQILKKCKEAVPQEGGKVIIVDVVLNMDL-THPYSKI 297
Query: 720 RXXXXXXXXXXXGGKERTKDEWRILLE 800
R GGKERT +EW+ L++
Sbjct: 298 RLTLDLDMMLNTGGKERTVEEWKKLID 324
>ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 207 bits (526), Expect = 9e-51
Identities = 106/263 (40%), Positives = 153/263 (58%)
Frame = +3
Query: 9 ENLYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGW 188
++L+R++RYL HM + +G+ + L +K ++K ++S+ +L + D+ + W
Sbjct: 63 DHLHRLMRYLVHMKIFTKETLDGEARYGLAPPAKFIVKGWDKSIVSIILVVTDKDFMAPW 122
Query: 189 HELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDI 368
H L D S T F K G++IW + PE N L N MACDT++++ A VQ C
Sbjct: 123 HCLKD---SLCGEGTAFEKALGRSIWTYMADHPEKNKLFNEGMACDTKLLISALVQDCKD 179
Query: 369 ILKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFESI 548
+ +GI LVD+GGGTG AM I KAFP +KC +DL GDMF+ I
Sbjct: 180 LFQGIMSLVDVGGGTGTAMRAIAKAFPHLKCTIYDLPHVIADSPDYPEVDRIAGDMFKHI 239
Query: 549 PPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARXX 728
P ADA+LLK +LH+W D EC+++LKRCKE++P GKVII+++V+D E LT+ R
Sbjct: 240 PSADAILLKCILHDWDDGECIEILKRCKESVPREGGKVIIVDIVVDL-ESKHPLTKTRLS 298
Query: 729 XXXXXXXXXGGKERTKDEWRILL 797
GGKERT+ EW+ LL
Sbjct: 299 LDLDMMVTTGGKERTEAEWKKLL 321
>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
Length = 346
Score = 207 bits (526), Expect = 9e-51
Identities = 115/265 (43%), Positives = 155/265 (58%), Gaps = 1/265 (0%)
Frame = +3
Query: 9 ENLYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGW 188
+ L+R++RYL HM L ++A + +SL +K LL+ E+S+ D +L I+D+ + W
Sbjct: 65 DRLFRIMRYLVHMELFKIDATT--QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPW 122
Query: 189 HELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDI 368
H L D DA F K GK+IW E PE N L N AMACDTR+V A C
Sbjct: 123 HHLGDGLTGNCDA---FEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKS 179
Query: 369 ILK-GIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFES 545
I GIK LVD+GGGTG A+ I KAFP+IKC +DL GDMF+S
Sbjct: 180 IFSDGIKTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKS 239
Query: 546 IPPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARX 725
IP ADA+ +K +LH+W D EC+++LKRCKE +P +GKVII+++V+D D + R
Sbjct: 240 IPSADAIFMKCILHDWNDEECIQILKRCKEALP-KDGKVIIVDVVIDMD-STHPYAKIRL 297
Query: 726 XXXXXXXXXXGGKERTKDEWRILLE 800
GGKERTK+EW+IL +
Sbjct: 298 TLDLDMMLNTGGKERTKEEWKILFD 322
>gb|AKN62836.1| norcoclaurine 6-O-methyltransferase, partial [Papaver
pseudo-orientale]
Length = 343
Score = 206 bits (523), Expect = 2e-50
Identities = 114/265 (43%), Positives = 155/265 (58%), Gaps = 1/265 (0%)
Frame = +3
Query: 9 ENLYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGW 188
+ L+R++RYL HM L ++A + +SL +K LL+ E+S+ D +L I+D+ + W
Sbjct: 63 DRLFRIMRYLVHMELFKIDATT--QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPW 120
Query: 189 HELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDI 368
H L D DA F K GK+IW E PE N L N AMACDTR+V A C
Sbjct: 121 HHLGDGLTGNCDA---FEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKS 177
Query: 369 ILK-GIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFES 545
I GI+ LVD+GGGTG A+ I KAFP+IKC +DL GDMF+S
Sbjct: 178 IFSDGIRTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKS 237
Query: 546 IPPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARX 725
IP ADA+ +K +LH+W D EC+++LKRCKE +P +GKVII+++V+D D + R
Sbjct: 238 IPSADAIFMKCILHDWSDEECIQILKRCKEALP-KDGKVIIVDVVIDMD-STHPYAKIRL 295
Query: 726 XXXXXXXXXXGGKERTKDEWRILLE 800
GGKERTK+EW+IL +
Sbjct: 296 TLDLDMMLNTGGKERTKEEWKILFD 320
>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
glaucum]
Length = 350
Score = 204 bits (519), Expect = 6e-50
Identities = 104/263 (39%), Positives = 152/263 (57%)
Frame = +3
Query: 15 LYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGWHE 194
LYR+LRYL HM L ++ +G+ + L +K L+K ++ + +L I D+ + WH
Sbjct: 66 LYRVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPWHY 125
Query: 195 LTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDIIL 374
L + L+ T F K G IW+ E PE N L N MA DTR++M A V+ C +
Sbjct: 126 LKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSSMF 185
Query: 375 KGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFESIPP 554
GI +VD+GGGTG A+ I KAFP IKC +DL GDMF+ IP
Sbjct: 186 DGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMFKYIPN 245
Query: 555 ADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARXXXX 734
ADA+++K +LH+W D EC+++LKRCK+ +P + GKVIII+++LD + + T+ R
Sbjct: 246 ADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDV-KSEHPYTKMRLTLD 304
Query: 735 XXXXXXXGGKERTKDEWRILLEE 803
GGKERT++EW+ L+ +
Sbjct: 305 LDMMLNTGGKERTEEEWKKLIHD 327
>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase
[Papaver somniferum]
Length = 346
Score = 201 bits (511), Expect = 5e-49
Identities = 113/265 (42%), Positives = 152/265 (57%), Gaps = 1/265 (0%)
Frame = +3
Query: 9 ENLYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGW 188
+ L+R++RYL HM L ++A + +SL +K LL+ E+S+ D +L I+D+ + W
Sbjct: 65 DRLFRIMRYLVHMELFKIDATT--QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPW 122
Query: 189 HELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDI 368
H L D DA F K GK+IW E PE N L N AMACDTR+V A C
Sbjct: 123 HHLGDGLTGNCDA---FEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKS 179
Query: 369 ILK-GIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFES 545
I GI LVD+GGGTG A+ I KAFP+IKC +DL GDMF+S
Sbjct: 180 IFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKS 239
Query: 546 IPPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARX 725
IP ADA+ +K +LH+W D EC+++LKRCKE +P GKVII+++V+D D + R
Sbjct: 240 IPSADAIFMKCILHDWNDDECIQILKRCKEALP-KVGKVIIVDVVIDMD-STHPYAKIRL 297
Query: 726 XXXXXXXXXXGGKERTKDEWRILLE 800
GGKERTK+EW+ L +
Sbjct: 298 TLDLDMMLNTGGKERTKEEWKTLFD 322
>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
Length = 351
Score = 200 bits (508), Expect = 1e-48
Identities = 105/266 (39%), Positives = 157/266 (59%), Gaps = 2/266 (0%)
Frame = +3
Query: 9 ENLYRMLRYLSHMNLIAVNAAEGDET--FSLTGISKLLLKNQERSLKDWVLGIDDEHSIN 182
+ LYR++RYL HM L E +E+ +SL +K L+K E+S+ +L I+D+ ++
Sbjct: 66 DRLYRVMRYLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLINDKDFMS 125
Query: 183 GWHELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGC 362
WH L + ++ +A F K GK+IW+ P+ N + N MACD+R+V A V C
Sbjct: 126 PWHHLKEGLVNNSNA---FDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNEC 182
Query: 363 DIILKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFE 542
I +GI+ LVD+GGGTG A+ + KAFP IKC +DL G+MF+
Sbjct: 183 GNIFQGIQTLVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGEMFK 242
Query: 543 SIPPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQAR 722
SIP ADA+L+K +LH+W D EC+++LK+CKE +P GKVII+++VLD E ++ R
Sbjct: 243 SIPSADAILMKCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDM-ESKHPYSKVR 301
Query: 723 XXXXXXXXXXXGGKERTKDEWRILLE 800
GKERT++EW+ L++
Sbjct: 302 LTLDLDMMLNTEGKERTEEEWKKLID 327
>gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353]
Length = 348
Score = 199 bits (507), Expect = 1e-48
Identities = 112/265 (42%), Positives = 151/265 (56%), Gaps = 1/265 (0%)
Frame = +3
Query: 9 ENLYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGW 188
+ L+R++RYL HM L ++A + +SL +K LL+ E+S+ D +L I+D+ + W
Sbjct: 65 DRLFRIMRYLVHMELFKIDATT--QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPW 122
Query: 189 HELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDI 368
H L D DA F K GK+IW PE N L N AMACDTR+V A C
Sbjct: 123 HHLGDGLTGNCDA---FEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKS 179
Query: 369 ILK-GIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFES 545
I GI LVD+GGGTG A+ I KAFP+IKC +DL GDMF+S
Sbjct: 180 IFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKS 239
Query: 546 IPPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARX 725
IP ADA+ +K +LH+W D EC+++LKRCKE +P GKVII+++V+D D + R
Sbjct: 240 IPSADAIFMKCILHDWNDDECIQILKRCKEALP-KGGKVIIVDVVIDMD-STHPYAKIRL 297
Query: 726 XXXXXXXXXXGGKERTKDEWRILLE 800
GGKERTK+EW+ L +
Sbjct: 298 TLDLDMMLNTGGKERTKEEWKTLFD 322
>sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase;
Short=Ps6OMT gi|33286374|gb|AAQ01669.1|
(R,S)-norcoclaurine 6-O-methyltransferase [Papaver
somniferum] gi|571330878|gb|AHF27395.1|
(R,S)-norcoclaurine 6-O-methyltransferase [synthetic
construct]
Length = 346
Score = 199 bits (507), Expect = 1e-48
Identities = 112/265 (42%), Positives = 151/265 (56%), Gaps = 1/265 (0%)
Frame = +3
Query: 9 ENLYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGW 188
+ L+R++RYL HM L ++A + +SL +K LL+ E+S+ D +L I+D+ + W
Sbjct: 65 DRLFRIMRYLVHMELFKIDATT--QKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPW 122
Query: 189 HELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDI 368
H L D DA F K GK+IW PE N L N AMACDTR+V A C
Sbjct: 123 HHLGDGLTGNCDA---FEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKS 179
Query: 369 ILK-GIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFES 545
I GI LVD+GGGTG A+ I KAFP+IKC +DL GDMF+S
Sbjct: 180 IFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKS 239
Query: 546 IPPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARX 725
IP ADA+ +K +LH+W D EC+++LKRCKE +P GKVII+++V+D D + R
Sbjct: 240 IPSADAIFMKCILHDWNDDECIQILKRCKEALP-KGGKVIIVDVVIDMD-STHPYAKIRL 297
Query: 726 XXXXXXXXXXGGKERTKDEWRILLE 800
GGKERTK+EW+ L +
Sbjct: 298 TLDLDMMLNTGGKERTKEEWKTLFD 322
>gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyllum hexandrum]
Length = 350
Score = 199 bits (506), Expect = 2e-48
Identities = 107/265 (40%), Positives = 150/265 (56%), Gaps = 2/265 (0%)
Frame = +3
Query: 12 NLYRMLRYLSHMNLIA--VNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSING 185
+LYR++RYL H+N+ ++ +G+ + L +K L+K + + +LGI D+ +
Sbjct: 65 SLYRVMRYLVHINIFTKTLDENDGETKYGLAAPAKFLVKGWDNCMVGSILGITDKVFMEP 124
Query: 186 WHELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCD 365
W+ + D A T F GK IWE E PE N L N AMACDT M+M A + C
Sbjct: 125 WYYIKDEL--APGTGTAFELALGKDIWEYMGENPEKNKLFNAAMACDTSMIMSALISECK 182
Query: 366 IILKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFES 545
GI+ LVD+GGGTG A I +AFP IKC +DL GDMF+
Sbjct: 183 DKFNGIRTLVDVGGGTGTAARNIARAFPNIKCTVYDLPHVIADSPVYPEINRVSGDMFKC 242
Query: 546 IPPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARX 725
IP ADA+L+K +LH+W+D EC+++LKRCKE +P + GKVIII++VLD E + T+ R
Sbjct: 243 IPNADAILMKCILHDWEDKECIEILKRCKEAVPVDGGKVIIIDVVLD-GESEHPYTKVRL 301
Query: 726 XXXXXXXXXXGGKERTKDEWRILLE 800
GKERTK+ W+ L +
Sbjct: 302 NSDLDMMLNTEGKERTKEGWKKLFK 326
>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase; Short=6-OMT
gi|9711914|dbj|BAB08004.1|
S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase [Coptis japonica]
Length = 347
Score = 198 bits (503), Expect = 4e-48
Identities = 104/263 (39%), Positives = 150/263 (57%)
Frame = +3
Query: 15 LYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGWHE 194
LYR++RYL HM L + +G+ + L +K L+K ++ + +L I D+ + WH
Sbjct: 65 LYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPWHY 124
Query: 195 LTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDIIL 374
L D + ++ T F K G IW E PE N L N AMA D+R++M A V+ C I
Sbjct: 125 LKDGL--SGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIF 182
Query: 375 KGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFESIPP 554
GI LVD+GGGTG A+ I AFP IKC +DL GDMF+ IP
Sbjct: 183 NGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIPK 242
Query: 555 ADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARXXXX 734
ADA+++K +LH+W D EC+++LKRCKE +P GKVII+++VL+ + + T+ R
Sbjct: 243 ADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNV-QSEHPYTKMRLTLD 301
Query: 735 XXXXXXXGGKERTKDEWRILLEE 803
GGKERT++EW+ L+ +
Sbjct: 302 LDMMLNTGGKERTEEEWKKLIHD 324
>gb|AFK40900.1| unknown [Lotus japonicus]
Length = 357
Score = 197 bits (502), Expect = 6e-48
Identities = 105/270 (38%), Positives = 153/270 (56%), Gaps = 5/270 (1%)
Frame = +3
Query: 3 KAENLYRMLRYLSHMNLIA---VNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEH 173
KA N+ R++R + H + + E + ++LT S LLLK S+ ++L +D
Sbjct: 66 KAGNIPRLMRVMIHSGFFSQHNITENELEVEYTLTDDSILLLKGHPFSILPFLLCSNDPI 125
Query: 174 SINGWHELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFV 353
WH L+ + +D TPF HG W+ A P+VN L+N +MACD +++ F+
Sbjct: 126 LKKPWHHLSTWF--KNDDLTPFETAHGMLPWDYANRDPKVNNLLNESMACDAQLIAGVFI 183
Query: 354 QGCDIILKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGD 533
+ C +L G++ LVD+GGGTG I K+FP +KC FDL GGD
Sbjct: 184 EKCKDMLNGLESLVDVGGGTGTMARAIAKSFPRLKCTVFDLPHVVADLQGSENLEYIGGD 243
Query: 534 MFESIPPADALLLKFMLHNWKDAECMKLLKRCKETIPA--NNGKVIIIEMVLDQDEGDDD 707
MF+ IPPADA+LLK++LH+W D EC+K+LK CKE I + GKVIII+MV+++D+GDD
Sbjct: 244 MFKEIPPADAILLKWILHDWSDEECLKILKNCKEAIKSKGKEGKVIIIDMVIEEDKGDDK 303
Query: 708 LTQARXXXXXXXXXXXGGKERTKDEWRILL 797
+ + GKER+ EW IL+
Sbjct: 304 SVETQLLFDMGMMVLTTGKERSMKEWGILI 333
>ref|XP_012088195.1| PREDICTED: trans-resveratrol di-O-methyltransferase-like [Jatropha
curcas]
Length = 361
Score = 196 bits (498), Expect = 2e-47
Identities = 104/266 (39%), Positives = 145/266 (54%), Gaps = 5/266 (1%)
Frame = +3
Query: 3 KAENLYRMLRYLSHMNLIAVNAAEGD----ETFSLTGISKLLLKNQERSLKDWVLGIDDE 170
K + R++R L H + E + E + LT SKLL+K SLK ++L + D
Sbjct: 70 KTHAIPRLVRILVHSGFLDKEKTEENSQETEGYVLTNASKLLVKEHPFSLKTFLLAMLDP 129
Query: 171 HSINGWHELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAF 350
W LT + L+ D PTPF HG+ IW+ A PE+N+ N AMACD +MV
Sbjct: 130 VLTGPWDHLTTWFLNDD--PTPFQTCHGRGIWKYAGHNPELNSSFNEAMACDAKMVASIL 187
Query: 351 VQGCDIILKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGG 530
++ C+ + G+K LVD+GGGTG + +FP++ C FDL GG
Sbjct: 188 IKECEGVFDGLKSLVDVGGGTGTVAKALADSFPQLDCCVFDLPHVVAGLQDGDNLKFVGG 247
Query: 531 DMFESIPPADALLLKFMLHNWKDAECMKLLKRCKETIPA-NNGKVIIIEMVLDQDEGDDD 707
DMFE IPPADA+LLK+++H+W D +C+K+LK CKE I GK+III+MV++ + D D
Sbjct: 248 DMFEEIPPADAILLKWIMHDWHDEKCIKILKNCKEAIKGRKGGKLIIIDMVMETQKEDKD 307
Query: 708 LTQARXXXXXXXXXXXGGKERTKDEW 785
T+ GKER K EW
Sbjct: 308 ATETELFLDMLMIVLYNGKERNKKEW 333
>gb|KHG11439.1| RS-norcoclaurine 6-O-methyltransferase [Gossypium arboreum]
Length = 303
Score = 196 bits (498), Expect = 2e-47
Identities = 103/265 (38%), Positives = 150/265 (56%), Gaps = 2/265 (0%)
Frame = +3
Query: 15 LYRMLRYLSHMNLIAVNAAEGDET-FSLTGISKLLLKNQERSLKDWVLGIDDEHSINGWH 191
LYR++R++ H + FS T +S+LL+K E+S+ ++L + + WH
Sbjct: 17 LYRIMRFMVHYRIFKQEHINHHTVGFSSTPLSRLLIKGGEKSMVAFILFVSSPTWLAPWH 76
Query: 192 ELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDII 371
L+ L + +PF +GK +W AE P+ + L NNAM CD R+ + A ++GC +
Sbjct: 77 SLSARVLETGNNISPFEVANGKDLWSYAEANPDFSELFNNAMGCDARLTVQATIEGCPEV 136
Query: 372 LKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFESIP 551
G+K LVD+GGG G A+S +VKAFP I+ + FDL GGDMF SIP
Sbjct: 137 FDGVKSLVDVGGGNGTALSLLVKAFPWIRGINFDLPHVVAVAPKSDSIENVGGDMFMSIP 196
Query: 552 PADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQD-EGDDDLTQARXX 728
ADA L ++LH+W D EC+K+LK+C+E IP + GKVII+E VL++D EGD+
Sbjct: 197 NADAAFLMWVLHDWDDEECIKILKKCREAIPEDKGKVIIVEAVLEEDKEGDELGAVGLML 256
Query: 729 XXXXXXXXXGGKERTKDEWRILLEE 803
GKERT EW +L++
Sbjct: 257 DMVMMAITNKGKERTLKEWSYVLQQ 281
>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
[Thalictrum flavum subsp. glaucum]
Length = 348
Score = 195 bits (495), Expect = 4e-47
Identities = 101/264 (38%), Positives = 154/264 (58%)
Frame = +3
Query: 9 ENLYRMLRYLSHMNLIAVNAAEGDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSINGW 188
+NL+R+LRYL M L++ A D+ ++L I+KL+L+N++RS+ VLG+ + + W
Sbjct: 68 DNLFRILRYLVKMELLSYAA---DDKYALEPIAKLVLRNEKRSMVPMVLGMTQKDFMTPW 124
Query: 189 HELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCDI 368
H + D D+ T F K G TIWE E P+ + L N MA +TR++ + + G
Sbjct: 125 HSMKDGL--TDNGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSKD 182
Query: 369 ILKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFESI 548
+ + I LVD+GGG G + I AFP IKC FDL GGDMF+ +
Sbjct: 183 MFQSIDSLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKGM 242
Query: 549 PPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARXX 728
P A A+LLK +LH+W D + +K+LK+C++ +P + GKVII+++ LD+D D +L+ R
Sbjct: 243 PSAQAILLKLILHDWNDEDSIKILKQCRKAVPKDGGKVIIVDVALDED-SDHELSSTRLI 301
Query: 729 XXXXXXXXXGGKERTKDEWRILLE 800
GGKERTK++W L++
Sbjct: 302 LDIDMLVNTGGKERTKEDWEKLVK 325
>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase [Coptis chinensis]
Length = 350
Score = 194 bits (493), Expect = 6e-47
Identities = 100/265 (37%), Positives = 152/265 (57%), Gaps = 1/265 (0%)
Frame = +3
Query: 9 ENLYRMLRYLSHMNLIAVNAAE-GDETFSLTGISKLLLKNQERSLKDWVLGIDDEHSING 185
+NLYR+LRYL M ++ V ++ G++ ++L I+ LL +N +RS+ +LG+ + +
Sbjct: 67 DNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTP 126
Query: 186 WHELTDYCLSADDAPTPFVKLHGKTIWELAEEVPEVNALINNAMACDTRMVMPAFVQGCD 365
WH + D D T F K G TIWE E P+ + L N MA +TR++ + + G
Sbjct: 127 WHSMKD---GLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSR 183
Query: 366 IILKGIKKLVDIGGGTGAAMSYIVKAFPEIKCMAFDLXXXXXXXXXXXXXXXXGGDMFES 545
+ +GI LVD+GGG G + I AFP IKC FDL GGDMF+S
Sbjct: 184 DMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKS 243
Query: 546 IPPADALLLKFMLHNWKDAECMKLLKRCKETIPANNGKVIIIEMVLDQDEGDDDLTQARX 725
+P A A+LLK +LH+W D + +K+LK+C+ +P + GKVII+++ LD +E D +L+ R
Sbjct: 244 VPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALD-EESDHELSSTRL 302
Query: 726 XXXXXXXXXXGGKERTKDEWRILLE 800
GGKERTK+ W +++
Sbjct: 303 ILDIDMLVNTGGKERTKEVWEKIVK 327