BLASTX nr result
ID: Papaver27_contig00050498
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver27_contig00050498
(835 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006382571.1| hypothetical protein POPTR_0005s034102g, par... 47 3e-08
ref|XP_006369230.1| hypothetical protein POPTR_0001s19740g, part... 47 5e-08
ref|XP_006387777.1| hypothetical protein POPTR_0590s00210g [Popu... 48 3e-07
ref|XP_006296777.1| hypothetical protein CARUB_v10015822mg, part... 42 7e-07
ref|XP_003623432.1| Stachyose synthase [Medicago truncatula] gi|... 60 8e-07
ref|XP_003592894.1| hypothetical protein MTR_2g005460 [Medicago ... 50 9e-07
ref|XP_006601654.1| PREDICTED: uncharacterized protein LOC100791... 52 9e-07
ref|XP_003604996.1| hypothetical protein MTR_4g022640 [Medicago ... 51 1e-06
ref|XP_006293025.1| hypothetical protein CARUB_v10019305mg, part... 48 1e-06
ref|XP_006299881.1| hypothetical protein CARUB_v10016088mg, part... 45 2e-06
ref|XP_006371236.1| hypothetical protein POPTR_0019s07150g [Popu... 45 4e-06
ref|XP_006378895.1| hypothetical protein POPTR_0009s00290g [Popu... 49 4e-06
ref|XP_003609254.1| hypothetical protein MTR_4g113700 [Medicago ... 50 5e-06
ref|XP_006301986.1| hypothetical protein CARUB_v10022467mg, part... 47 5e-06
ref|XP_006375693.1| hypothetical protein POPTR_0014s19700g, part... 48 5e-06
ref|XP_003602362.1| hypothetical protein MTR_3g092560 [Medicago ... 57 7e-06
ref|XP_006375665.1| hypothetical protein POPTR_0014s19090g, part... 49 9e-06
>ref|XP_006382571.1| hypothetical protein POPTR_0005s034102g, partial [Populus
trichocarpa] gi|550337934|gb|ERP60368.1| hypothetical
protein POPTR_0005s034102g, partial [Populus
trichocarpa]
Length = 429
Score = 47.4 bits (111), Expect(3) = 3e-08
Identities = 23/51 (45%), Positives = 29/51 (56%)
Frame = +2
Query: 326 VWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
VW RFP LPL+ W CL +LA IGKP+ +D T + VLV+ D
Sbjct: 189 VWIRFPNLPLQCWSPICLSKLASVIGKPLHLDTPTSSMSRLSYARVLVETD 239
Score = 29.6 bits (65), Expect(3) = 3e-08
Identities = 14/50 (28%), Positives = 27/50 (54%)
Frame = +3
Query: 174 DKGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKIIRL 323
D G+ + NF +E+D V G + G+ L ++ FD +T +++R+
Sbjct: 138 DSGWLIFNFDSEMDMLEVLNGGPYSVHGRLLILKIMPEFFDFDTSEMLRM 187
Score = 27.3 bits (59), Expect(3) = 3e-08
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +3
Query: 582 CKSTGHEESECRTRKYNKLKKKTEAETDPT 671
C+S GH S C T +K +K+ + P+
Sbjct: 274 CRSLGHSTSSCATNSSHKRRKRGQPTPTPS 303
>ref|XP_006369230.1| hypothetical protein POPTR_0001s19740g, partial [Populus
trichocarpa] gi|550347685|gb|ERP65799.1| hypothetical
protein POPTR_0001s19740g, partial [Populus trichocarpa]
Length = 521
Score = 46.6 bits (109), Expect(3) = 5e-08
Identities = 23/51 (45%), Positives = 28/51 (54%)
Frame = +2
Query: 326 VWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
VW RFP LPL W CL +LA IGKP+ +D T + VLV+ D
Sbjct: 292 VWIRFPNLPLRCWSPICLSKLASVIGKPLHLDTPTSSMSRLSYARVLVETD 342
Score = 29.6 bits (65), Expect(3) = 5e-08
Identities = 14/50 (28%), Positives = 27/50 (54%)
Frame = +3
Query: 174 DKGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKIIRL 323
D G+ + NF +E+D V G + G+ L ++ FD +T +++R+
Sbjct: 241 DSGWLIFNFDSEMDMLEVLNGGPYSVHGRLLILKIMPEFFDFDTSEMLRM 290
Score = 27.3 bits (59), Expect(3) = 5e-08
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +3
Query: 582 CKSTGHEESECRTRKYNKLKKKTEAETDPT 671
C+S GH S C T +K +K+ + P+
Sbjct: 377 CRSLGHSTSSCATNSSHKRRKRGQPTPTPS 406
>ref|XP_006387777.1| hypothetical protein POPTR_0590s00210g [Populus trichocarpa]
gi|550308426|gb|ERP46691.1| hypothetical protein
POPTR_0590s00210g [Populus trichocarpa]
Length = 404
Score = 47.8 bits (112), Expect(4) = 3e-07
Identities = 23/51 (45%), Positives = 29/51 (56%)
Frame = +2
Query: 326 VWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
VW RFP LPL+ W CL +LA IGKP+ D T + VL++ID
Sbjct: 153 VWVRFPNLPLQCWSPLCLSKLASVIGKPVHSDTSTNSMTRLSYARVLIEID 203
Score = 25.8 bits (55), Expect(4) = 3e-07
Identities = 14/50 (28%), Positives = 24/50 (48%)
Frame = +3
Query: 174 DKGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKIIRL 323
D G+ + F +E D V G + GQ L ++ FD T +++R+
Sbjct: 102 DSGWLIFTFTSETDMFEVLSGGPYYVFGQPLILKIMPEFFDFATSEMVRM 151
Score = 23.5 bits (49), Expect(4) = 3e-07
Identities = 8/30 (26%), Positives = 15/30 (50%)
Frame = +3
Query: 582 CKSTGHEESECRTRKYNKLKKKTEAETDPT 671
C++ GH S C + +K K ++ P+
Sbjct: 238 CRTRGHTTSTCTNKSSHKRNKHSQTAPAPS 267
Score = 22.7 bits (47), Expect(4) = 3e-07
Identities = 7/10 (70%), Positives = 10/10 (100%)
Frame = +1
Query: 52 KEVWKFSLVG 81
K++WKFSL+G
Sbjct: 63 KDLWKFSLIG 72
>ref|XP_006296777.1| hypothetical protein CARUB_v10015822mg, partial [Capsella rubella]
gi|482565486|gb|EOA29675.1| hypothetical protein
CARUB_v10015822mg, partial [Capsella rubella]
Length = 333
Score = 42.4 bits (98), Expect(2) = 7e-07
Identities = 19/51 (37%), Positives = 29/51 (56%)
Frame = +2
Query: 326 VWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
VW R LP+ F+ + L +A +GKP+ VD T R E + F + V++D
Sbjct: 163 VWVRVSKLPVLFYHKAILMGIAEGLGKPLRVDLTTFRFERARFARICVEVD 213
Score = 38.1 bits (87), Expect(2) = 7e-07
Identities = 17/48 (35%), Positives = 27/48 (56%)
Frame = +3
Query: 171 MDKGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKI 314
+ + +F++ F NE D V G W+ G L VQ +P FDP+ ++I
Sbjct: 111 LPRQFFMVRFDNEADYMAVMTGGPWRLFGSVLMVQAWSPSFDPQHDEI 158
>ref|XP_003623432.1| Stachyose synthase [Medicago truncatula]
gi|355498447|gb|AES79650.1| Stachyose synthase [Medicago
truncatula]
Length = 676
Score = 60.5 bits (145), Expect = 8e-07
Identities = 26/57 (45%), Positives = 37/57 (64%)
Frame = +2
Query: 320 AMVWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDIDFQNQ 490
A VW R LP E+W E L +A +IG P+++DA T +H +S++ VLVDIDF +
Sbjct: 88 AQVWLRLLDLPQEYWLERTLMEIAGAIGAPLIIDAATQKHTFSHYARVLVDIDFSRR 144
>ref|XP_003592894.1| hypothetical protein MTR_2g005460 [Medicago truncatula]
gi|92893902|gb|ABE91952.1| Zinc finger, CCHC-type
[Medicago truncatula] gi|355481942|gb|AES63145.1|
hypothetical protein MTR_2g005460 [Medicago truncatula]
Length = 504
Score = 50.4 bits (119), Expect(2) = 9e-07
Identities = 24/52 (46%), Positives = 33/52 (63%)
Frame = +2
Query: 323 MVWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
+VW RFP L L ++DE L +A ++G+PI VD TL+ E F V V+ID
Sbjct: 175 VVWVRFPGLNLVYYDESFLLAMASALGRPIKVDTNTLKVERGKFARVCVEID 226
Score = 29.6 bits (65), Expect(2) = 9e-07
Identities = 15/49 (30%), Positives = 22/49 (44%)
Frame = +3
Query: 174 DKGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKIIR 320
D G+F++ F D+++V G W L V TP F K+ R
Sbjct: 125 DNGFFMVKFDQAADKEKVITGGPWLIFDHCLAVTHWTPEFASPNAKVDR 173
>ref|XP_006601654.1| PREDICTED: uncharacterized protein LOC100791212 [Glycine max]
Length = 484
Score = 52.4 bits (124), Expect(2) = 9e-07
Identities = 26/52 (50%), Positives = 32/52 (61%)
Frame = +2
Query: 323 MVWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
MVW RFP L L ++DE L LAR+IG PI VD+ TL +F + V ID
Sbjct: 178 MVWVRFPSLNLIYYDENILLALARAIGTPIKVDSNTLDVRRGHFARICVQID 229
Score = 27.7 bits (60), Expect(2) = 9e-07
Identities = 14/44 (31%), Positives = 21/44 (47%)
Frame = +3
Query: 183 YFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKI 314
++++ F DR++V G W L +Q TP F T KI
Sbjct: 131 FYMVKFDTTEDRQKVIEGGPWMIFDHYLTIQTWTPDFISPTAKI 174
>ref|XP_003604996.1| hypothetical protein MTR_4g022640 [Medicago truncatula]
gi|355506051|gb|AES87193.1| hypothetical protein
MTR_4g022640 [Medicago truncatula]
Length = 513
Score = 51.2 bits (121), Expect(2) = 1e-06
Identities = 25/52 (48%), Positives = 33/52 (63%)
Frame = +2
Query: 323 MVWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
+VW RFP L L ++DE L +A +IG+PI VD TL+ E F V V+ID
Sbjct: 172 VVWVRFPGLNLVYYDESFLLAMASAIGRPIKVDTNTLKVERGKFARVCVEID 223
Score = 28.1 bits (61), Expect(2) = 1e-06
Identities = 13/49 (26%), Positives = 23/49 (46%)
Frame = +3
Query: 174 DKGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKIIR 320
D G++++ F E D+++V G W L V +P F ++ R
Sbjct: 122 DNGFYMVKFDQEADKEKVVTGGPWMIFDHCLAVTHWSPEFASPEARVER 170
>ref|XP_006293025.1| hypothetical protein CARUB_v10019305mg, partial [Capsella rubella]
gi|482561732|gb|EOA25923.1| hypothetical protein
CARUB_v10019305mg, partial [Capsella rubella]
Length = 361
Score = 48.1 bits (113), Expect(2) = 1e-06
Identities = 20/53 (37%), Positives = 31/53 (58%)
Frame = +2
Query: 326 VWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDIDFQ 484
VW R +P+ F+ + L RLAR +GKP+ VD T+ E F + VD++ +
Sbjct: 166 VWIRLSNIPVNFYHKRLLMRLARGLGKPVKVDLTTMNFERGRFARICVDVNLK 218
Score = 31.2 bits (69), Expect(2) = 1e-06
Identities = 16/56 (28%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Frame = +3
Query: 156 GPIHTMD--KGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKII 317
G ++ +D + +F++ F+ E + G W+ G L V+ P FDP T++I+
Sbjct: 107 GAMYVVDLPRQFFMVRFELEDEYLEALTGGPWRIFGSYLMVKAWEPEFDPMTDEIV 162
>ref|XP_006299881.1| hypothetical protein CARUB_v10016088mg, partial [Capsella rubella]
gi|482568590|gb|EOA32779.1| hypothetical protein
CARUB_v10016088mg, partial [Capsella rubella]
Length = 340
Score = 45.1 bits (105), Expect(2) = 2e-06
Identities = 20/54 (37%), Positives = 31/54 (57%)
Frame = +2
Query: 326 VWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDIDFQN 487
VW R LP+ F+ + L +A +G PI VD TL+ E + F + V++D +N
Sbjct: 166 VWIRLSNLPVNFYHQFILMGIAEGLGTPIKVDLTTLQFERARFARICVEVDLKN 219
Score = 33.5 bits (75), Expect(2) = 2e-06
Identities = 18/55 (32%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +3
Query: 156 GPIHTMD--KGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKI 314
G +H MD + +F+I F+ E + G W+ G L Q +P FDP ++I
Sbjct: 107 GAMHVMDLPRQFFMIRFEKEEEYMAALTGGPWRVFGSCLLTQAWSPSFDPLRDEI 161
>ref|XP_006371236.1| hypothetical protein POPTR_0019s07150g [Populus trichocarpa]
gi|550316936|gb|ERP49033.1| hypothetical protein
POPTR_0019s07150g [Populus trichocarpa]
Length = 509
Score = 44.7 bits (104), Expect(4) = 4e-06
Identities = 21/51 (41%), Positives = 28/51 (54%)
Frame = +2
Query: 326 VWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
VW +FP L L+ W CL +LA IGKP+ D T + VLV++D
Sbjct: 360 VWVKFPNLSLQCWSPLCLSKLASVIGKPVHADTPTASMTRLSYARVLVEVD 410
Score = 25.8 bits (55), Expect(4) = 4e-06
Identities = 15/50 (30%), Positives = 24/50 (48%)
Frame = +3
Query: 174 DKGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKIIRL 323
D G+ + F +E+D V G + G L ++ FD +T +IRL
Sbjct: 309 DSGWLIFKFTSELDMLAVLNGGPYHVFGWLLILKHMPDYFDFDTSDMIRL 358
Score = 22.7 bits (47), Expect(4) = 4e-06
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 1 STCDIVVTILRATHIRAKEVWKFSLVG 81
S+C + + LR KE+W+FSL+G
Sbjct: 257 SSCHFLDSDLRGY----KELWQFSLIG 279
Score = 22.3 bits (46), Expect(4) = 4e-06
Identities = 10/31 (32%), Positives = 13/31 (41%)
Frame = +3
Query: 582 CKSTGHEESECRTRKYNKLKKKTEAETDPTN 674
CK+ GH C +K KK + P N
Sbjct: 445 CKTLGHSNYACTKSSSHKRKKPPQQLLLPPN 475
>ref|XP_006378895.1| hypothetical protein POPTR_0009s00290g [Populus trichocarpa]
gi|550330718|gb|ERP56692.1| hypothetical protein
POPTR_0009s00290g [Populus trichocarpa]
Length = 244
Score = 48.9 bits (115), Expect(2) = 4e-06
Identities = 24/51 (47%), Positives = 30/51 (58%)
Frame = +2
Query: 326 VWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
VW RFP LPL+ W CL +LA IGKP+ +D T + VLV+ID
Sbjct: 3 VWIRFPNLPLQCWSPICLSKLASVIGKPLHLDTPTSSMTRLSYARVLVEID 53
Score = 28.9 bits (63), Expect(2) = 4e-06
Identities = 15/52 (28%), Positives = 25/52 (48%), Gaps = 1/52 (1%)
Frame = +3
Query: 582 CKSTGHEESECRTRKYNKLKKKTEAETDPTNQQAIFDEMDEI-KNECRKKRE 734
C+S GH S C T +K KK+ + P+ E + + K R++R+
Sbjct: 88 CRSLGHSTSSCATNSNHKRKKRGQPTPTPSGCSNPSAETEAVEKQPLREERQ 139
>ref|XP_003609254.1| hypothetical protein MTR_4g113700 [Medicago truncatula]
gi|355510309|gb|AES91451.1| hypothetical protein
MTR_4g113700 [Medicago truncatula]
Length = 484
Score = 50.4 bits (119), Expect(2) = 5e-06
Identities = 24/52 (46%), Positives = 33/52 (63%)
Frame = +2
Query: 323 MVWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
+VW RFP L L ++DE L +A ++G+PI VD TL+ E F V V+ID
Sbjct: 175 VVWVRFPGLNLVYYDESLLLAMATTLGQPIKVDTNTLKVERGKFARVCVEID 226
Score = 26.9 bits (58), Expect(2) = 5e-06
Identities = 13/49 (26%), Positives = 22/49 (44%)
Frame = +3
Query: 174 DKGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKIIR 320
D G++++ F D+++V G W L V +P F K+ R
Sbjct: 125 DNGFYMVKFDQAADKEKVITGGPWLIFDHCLAVTHWSPEFASPNAKVDR 173
>ref|XP_006301986.1| hypothetical protein CARUB_v10022467mg, partial [Capsella rubella]
gi|482570696|gb|EOA34884.1| hypothetical protein
CARUB_v10022467mg, partial [Capsella rubella]
Length = 421
Score = 47.0 bits (110), Expect(2) = 5e-06
Identities = 22/51 (43%), Positives = 31/51 (60%)
Frame = +2
Query: 326 VWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
VW RF LP+ + + L LA+++GKP+ VDA TL E F V V++D
Sbjct: 153 VWVRFSNLPVALYHQTILLGLAQALGKPLRVDANTLNFERPRFARVCVEVD 203
Score = 30.4 bits (67), Expect(2) = 5e-06
Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
Frame = +3
Query: 162 IHTMDKGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFDPETEKI--IRLW 326
+ + + +F++ F+ E + G WKA G L V+ +P F+P + I I +W
Sbjct: 98 VTNLPRQFFMVRFEVEEEYLTALTGGPWKAFGSYLMVRAWSPHFEPSRDDITTIPVW 154
>ref|XP_006375693.1| hypothetical protein POPTR_0014s19700g, partial [Populus
trichocarpa] gi|550324566|gb|ERP53490.1| hypothetical
protein POPTR_0014s19700g, partial [Populus trichocarpa]
Length = 367
Score = 47.8 bits (112), Expect(2) = 5e-06
Identities = 21/51 (41%), Positives = 29/51 (56%)
Frame = +2
Query: 326 VWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
+W RFP LPL+ W CL +L IGKP+ D+ T + VL++ID
Sbjct: 122 IWVRFPNLPLQCWSPPCLSKLVSVIGKPVHADSPTSSMTRLSYARVLIEID 172
Score = 29.6 bits (65), Expect(2) = 5e-06
Identities = 14/63 (22%), Positives = 26/63 (41%)
Frame = +3
Query: 582 CKSTGHEESECRTRKYNKLKKKTEAETDPTNQQAIFDEMDEIKNECRKKREVKNQTVQPK 761
CK+ GH S C + +K KK A T P+ + + + + ++ ++ P
Sbjct: 207 CKTLGHSTSACNSASSHKRKKHPPAPTAPSGYSNPSADTEAVAQQSPREEPHGEPSIDPM 266
Query: 762 QTE 770
E
Sbjct: 267 AAE 269
>ref|XP_003602362.1| hypothetical protein MTR_3g092560 [Medicago truncatula]
gi|355491410|gb|AES72613.1| hypothetical protein
MTR_3g092560 [Medicago truncatula]
Length = 361
Score = 57.4 bits (137), Expect = 7e-06
Identities = 24/53 (45%), Positives = 35/53 (66%)
Frame = +2
Query: 320 AMVWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDID 478
+ VW RF YLPLE+W + LF +A +IG PI++D H + ++ VLVDI+
Sbjct: 170 SQVWVRFHYLPLEYWHPKFLFEIAGAIGTPILIDENNTIHFFGHYARVLVDIN 222
>ref|XP_006375665.1| hypothetical protein POPTR_0014s19090g, partial [Populus
trichocarpa] gi|550324538|gb|ERP53462.1| hypothetical
protein POPTR_0014s19090g, partial [Populus trichocarpa]
Length = 377
Score = 49.3 bits (116), Expect(2) = 9e-06
Identities = 22/55 (40%), Positives = 31/55 (56%)
Frame = +2
Query: 323 MVWCRFPYLPLEFWDEECLFRLARSIGKPIVVDARTLRHEYSYFVAVLVDIDFQN 487
+VW R P LPLE W CL +++ IGKPI D TL F V+++++ N
Sbjct: 256 LVWVRLPNLPLECWSPACLSKISSVIGKPIRCDDLTLSMSRVSFARVMIEVNLSN 310
Score = 27.3 bits (59), Expect(2) = 9e-06
Identities = 13/45 (28%), Positives = 22/45 (48%)
Frame = +3
Query: 162 IHTMDKGYFVINFQNEVDRKRVNYEGLWKAKGQQLKVQE*TPMFD 296
+H D G+ V F + + RV G + +G+ L ++ P FD
Sbjct: 202 LHIHDSGWLVFRFSSATNMGRVLCRGPYSFQGKPLVLKPMPPYFD 246