BLASTX nr result
ID: Papaver27_contig00027782
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver27_contig00027782
(835 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; Alt... 542 e-152
gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum] 393 e-107
sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase;... 378 e-102
sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase;... 350 3e-94
sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Sh... 300 6e-79
dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta] 298 1e-78
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g... 297 3e-78
gb|ACO90219.1| stylopine synthase [Eschscholzia californica] 293 4e-77
sp|Q50LH3.1|C7192_ESCCA RecName: Full=(S)-stylopine synthase 1; ... 293 5e-77
gb|AHF65153.1| stylopine synthase, partial [synthetic construct] 291 2e-76
gb|ADB89214.1| stylopine synthase [Papaver somniferum] 291 2e-76
gb|AGL76711.1| (S)-canadine synthase, partial [Coptis chinensis] 291 3e-76
dbj|BAG75116.1| cytochrome P450 [Eschscholzia californica] 290 4e-76
sp|Q50LH4.1|C7193_ESCCA RecName: Full=(S)-stylopine synthase 2; ... 290 4e-76
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt... 288 2e-75
gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum] 283 6e-74
dbj|BAG75114.1| cytochrome P450 [Eschscholzia californica] 283 7e-74
gb|AFB74615.1| cytochrome P450 [Papaver somniferum] 280 5e-73
dbj|BAG75115.1| cytochrome P450 [Eschscholzia californica] 279 8e-73
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta] 272 1e-70
>sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; AltName: Full=Cytochrome P450
719B1 gi|148841090|gb|ABR14720.1| salutaridine synthase
[Papaver somniferum]
Length = 505
Score = 542 bits (1396), Expect = e-152
Identities = 274/278 (98%), Positives = 275/278 (98%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP
Sbjct: 205 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 264
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
HIV NLPANSFL+FLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLV EQEIQEK
Sbjct: 265 HIVPNLPANSFLYFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVGEQEIQEK 324
Query: 475 LYREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIV 296
LYREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIV
Sbjct: 325 LYREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIV 384
Query: 295 KGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMRIC 116
KGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPF AGMRIC
Sbjct: 385 KGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFGAGMRIC 444
Query: 115 GGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
GGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI
Sbjct: 445 GGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 482
>gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum]
Length = 492
Score = 393 bits (1009), Expect = e-107
Identities = 190/276 (68%), Positives = 229/276 (82%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF +ED V+G+ ALDE+V ISG ASLADAFK + LPS +K +R ++A + R NLI+P
Sbjct: 196 DFVDEDFVVGMHQALDELVSISGYASLADAFKFCENLPSHKKTVRAVHAIKIRFDNLIRP 255
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
HIVSN P N++LHFL SQD+S+++I S +LE++ LGVDSTA+T VWAL+FLV EQ+IQEK
Sbjct: 256 HIVSNPPTNTYLHFLLSQDFSEDVIISAILEVYDLGVDSTASTTVWALTFLVREQKIQEK 315
Query: 475 LYREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIV 296
LYREINN TGG++PVKV DL +LPYLQAVMKET+RMKPIAP+A+PH A+KDT+ G++I
Sbjct: 316 LYREINNVTGGKKPVKVEDLNKLPYLQAVMKETMRMKPIAPMAIPHKASKDTSLMGKKIN 375
Query: 295 KGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMRIC 116
KG VMVNLYAIHH+P VFP PYKFMPERFLKD NSDG GDI MESSL+ FSAGMRIC
Sbjct: 376 KGAVVMVNLYAIHHNPAVFPEPYKFMPERFLKDANSDGSLGDIKKMESSLLAFSAGMRIC 435
Query: 115 GGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSE 8
G+EL K +AF LAS+VNEFKWDC +EGKLPDLSE
Sbjct: 436 AGMELGKLQLAFGLASLVNEFKWDCFAEGKLPDLSE 471
>sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
Full=Cytochrome P450 719A14 gi|148841094|gb|ABR14722.1|
cheilanthifoline synthase [Argemone mexicana]
Length = 494
Score = 378 bits (971), Expect = e-102
Identities = 182/276 (65%), Positives = 229/276 (82%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF +E LV+G+ ALD++VRISG ASLADAFK + LPS +K+IR+++ + RV NLI+P
Sbjct: 198 DFQDEKLVVGMHHALDDLVRISGYASLADAFKFCENLPSHKKSIREVHEVKKRVENLIRP 257
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
HIVSN P N++L+FL +QD++++II S +LE++ LGVDSTA+T VWAL+FLV EQEIQEK
Sbjct: 258 HIVSNPPTNTYLYFLKTQDFNEDIIISAILEVYDLGVDSTASTTVWALTFLVREQEIQEK 317
Query: 475 LYREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIV 296
LYREI N TGG+R VKV D+ ++PYLQAVMKET+RMKPIAP+A+PH +KDT+ G++I
Sbjct: 318 LYREIVNVTGGKRSVKVEDVNKMPYLQAVMKETMRMKPIAPMAIPHKTSKDTSLMGKKIN 377
Query: 295 KGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMRIC 116
KG+ +MVNLYAIHH+P VFP PYKFMPERFLKDVNSD G+I TMESSL+ FSAGMRIC
Sbjct: 378 KGSVIMVNLYAIHHNPKVFPEPYKFMPERFLKDVNSDESLGNIKTMESSLLAFSAGMRIC 437
Query: 115 GGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSE 8
G+EL K +AF LAS+V+EFKW C +GKLPDLSE
Sbjct: 438 AGMELGKLQLAFGLASLVHEFKWSCSVDGKLPDLSE 473
>sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
Full=Cytochrome P450 719A5 gi|209571368|dbj|BAG75113.1|
cheilanthifoline synthase [Eschscholzia californica]
Length = 490
Score = 350 bits (899), Expect = 3e-94
Identities = 167/275 (60%), Positives = 219/275 (79%)
Frame = -1
Query: 832 FSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQPH 653
F++E+ V GV ALD++VRISG ASLADAFK + LPS +K+IR+++ +RV NL++P+
Sbjct: 196 FNDENFVKGVHLALDDLVRISGYASLADAFKFCENLPSHKKSIREVHEVNERVVNLVKPY 255
Query: 652 IVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEKL 473
+V N P N++L+FL SQ +SDE+I S VLE++ LGVDSTA+TAVWAL+FLV E +QEKL
Sbjct: 256 LVKNPPTNTYLYFLNSQKFSDEVIISAVLEVYDLGVDSTASTAVWALTFLVREPRVQEKL 315
Query: 472 YREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIVK 293
Y+EI + TGG+R VKV D+ +LPYLQAVMKET+RMKPIAP+A+PH ++DT+ G+++ K
Sbjct: 316 YKEIIDLTGGERSVKVEDVSKLPYLQAVMKETMRMKPIAPMAIPHKTSRDTSLMGKKVNK 375
Query: 292 GTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMRICG 113
GT +MVNLYAIHH+P VFP PYKF+PERFL+ + ++GDI ME SL+PFSAGMRIC
Sbjct: 376 GTSIMVNLYAIHHNPKVFPEPYKFIPERFLQ--GQESKYGDIKEMEQSLLPFSAGMRICA 433
Query: 112 GVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSE 8
G+EL K F+LAS+V FKW C +GKLPDLSE
Sbjct: 434 GMELGKLQYGFSLASLVEAFKWTCAVDGKLPDLSE 468
>sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Short=STS; AltName:
Full=Cytochrome P450 719A13 gi|148841092|gb|ABR14721.1|
stylopine synthase [Argemone mexicana]
Length = 504
Score = 300 bits (767), Expect = 6e-79
Identities = 146/280 (52%), Positives = 202/280 (72%), Gaps = 2/280 (0%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF N++ V + ++E++R+SG A LA+AF AKYLPS +K +R++ RV NL++P
Sbjct: 210 DFDNDEYVEEMHHTIEELIRVSGYARLAEAFYYAKYLPSHKKAVREVLQANQRVQNLVRP 269
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+ N P N++LHFL SQ+Y DE+I + E + LGVDST++T WAL++L+ E +QEK
Sbjct: 270 LLSLNSPTNTYLHFLRSQNYEDEVIIFAIFEAYLLGVDSTSSTTAWALAYLIREPNVQEK 329
Query: 475 LYREINNRT--GGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRR 302
LY E+ N T ++ VK DL +L YLQAV+KET+RMKPIAPLA+PH A ++T+ GR+
Sbjct: 330 LYEELKNFTNDNDRKMVKFEDLNKLQYLQAVVKETMRMKPIAPLAIPHKACRETSLMGRK 389
Query: 301 IVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMR 122
+ +GT+VMVN+YA+HH+ NV+ PYKF PERFL+ N DG G ME SL+PFSAGMR
Sbjct: 390 VNQGTRVMVNIYALHHNQNVWKEPYKFNPERFLQK-NQDGVDG--KAMEQSLLPFSAGMR 446
Query: 121 ICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
IC G+EL K +FALA++VN FKW CVS+G PD+S+ +
Sbjct: 447 ICAGMELGKLQFSFALANLVNAFKWSCVSDGVFPDMSDQL 486
>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 491
Score = 298 bits (764), Expect = 1e-78
Identities = 146/277 (52%), Positives = 197/277 (71%)
Frame = -1
Query: 832 FSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQPH 653
F N+ V V +++++R+SG A L +AF AKYLPSQ+K R+ Y + R+ L++P
Sbjct: 199 FDNDKFVESVLYEIEDVLRLSGYARLTEAFYYAKYLPSQKKVAREAYLAKYRIEELMRPL 258
Query: 652 IVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEKL 473
+ SN P NS+L+FL SQ+YS EII E+F LGVDST++TA WAL++L+ EQ +QEKL
Sbjct: 259 LSSNPPTNSYLYFLQSQNYSKEIIIFCTFELFLLGVDSTSSTATWALAYLIHEQAVQEKL 318
Query: 472 YREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIVK 293
+I G VK+ DL +L YLQAV+KET+RMKPIAPLA+PH A DTT G ++ +
Sbjct: 319 NEDIRMTIGDVDLVKIEDLSKLKYLQAVVKETMRMKPIAPLAIPHKTANDTTLMGTKVAR 378
Query: 292 GTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMRICG 113
GT++MVNLYA+HH+ +++P PYKFMPERFL+ N + ME S +PF AGMRIC
Sbjct: 379 GTQIMVNLYALHHNEDIWPEPYKFMPERFLQGENVS---ANNKAMEQSFLPFGAGMRICA 435
Query: 112 GVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
G++L K +AFALA++VN FKW CV EGKLPD+SE +
Sbjct: 436 GMDLGKLQIAFALANLVNAFKWSCVDEGKLPDMSEQL 472
>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
Length = 492
Score = 297 bits (761), Expect = 3e-78
Identities = 146/277 (52%), Positives = 198/277 (71%)
Frame = -1
Query: 832 FSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQPH 653
F ++ V + +D+++RISG A LA+AF AKYLPS +K R+ Y + RV +L+ P
Sbjct: 200 FDDDKFVESMHFEIDDIIRISGYARLAEAFYYAKYLPSHKKAEREAYLVKCRVEDLVSPL 259
Query: 652 IVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEKL 473
+ SN P NS+L+FL SQ +S+E+I + E++ LGVDST++T WAL++L+ EQ IQEKL
Sbjct: 260 LSSNPPTNSYLYFLLSQRFSEEVIIFCIFELYLLGVDSTSSTTTWALAYLIREQAIQEKL 319
Query: 472 YREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIVK 293
Y++ G VK+ D+ +L YLQAV+KET+RMKPIAPLA+PH AKDTT G ++ K
Sbjct: 320 YQDTRMTLGDVDLVKIEDVSKLKYLQAVVKETMRMKPIAPLAIPHKTAKDTTLMGSKVAK 379
Query: 292 GTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMRICG 113
GT++MVNLYA+HH+ N++ PYKFMPERFL+ DG + ME S +PFSAGMRIC
Sbjct: 380 GTRIMVNLYALHHNQNIWTNPYKFMPERFLQ--GEDGSASN-KAMEQSFLPFSAGMRICA 436
Query: 112 GVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
G++L K AFALA++VN FKW CV EGK PD+SE +
Sbjct: 437 GMDLGKLQFAFALANLVNAFKWSCVEEGKFPDMSEEL 473
>gb|ACO90219.1| stylopine synthase [Eschscholzia californica]
Length = 495
Score = 293 bits (751), Expect = 4e-77
Identities = 143/280 (51%), Positives = 201/280 (71%), Gaps = 2/280 (0%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF+N+ V + A++E++R+SG A LA+AF AKYLPS +K +R++ + RV NL+ P
Sbjct: 201 DFNNDKYVDDMHLAIEELIRVSGYARLAEAFYYAKYLPSHKKAVREVEEAQRRVQNLVSP 260
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+ N P N++LHFL SQ Y DE+I + E + LGVDST+ T WAL+FL+ E +QEK
Sbjct: 261 FLSLNPPTNTYLHFLRSQKYDDEVIIFAIFEAYLLGVDSTSLTTAWALAFLIRESNVQEK 320
Query: 475 LYREINNRTG--GQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRR 302
LY+E+ + ++ +KV D+ +L YLQAV+KET+RMKPIAPLA+PH A +DT+ G++
Sbjct: 321 LYQELESFASKMDRKILKVEDINKLQYLQAVIKETMRMKPIAPLAIPHKACRDTSLMGKK 380
Query: 301 IVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMR 122
I KGT+VMVN+YA+HH+ NVF P+KFMPERF+K D + + ME SL+PFSAGMR
Sbjct: 381 IDKGTRVMVNIYALHHNKNVFNDPFKFMPERFMK---VDSQDANGKAMEQSLLPFSAGMR 437
Query: 121 ICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
IC G+EL K +FALA++ FKW CV++G LPD+S+ +
Sbjct: 438 ICAGMELGKLQFSFALANLAYAFKWSCVADGVLPDMSDQL 477
>sp|Q50LH3.1|C7192_ESCCA RecName: Full=(S)-stylopine synthase 1; Short=STS; AltName:
Full=Cytochrome P450 719A2; Short=EcCYP719A2;
Short=EcCYPB gi|63108256|dbj|BAD98250.1| stylopine
synthase [Eschscholzia californica]
Length = 495
Score = 293 bits (750), Expect = 5e-77
Identities = 143/280 (51%), Positives = 201/280 (71%), Gaps = 2/280 (0%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF+N+ V + A++E++R+SG A LA+AF AKYLPS +K +R++ + RV NL+ P
Sbjct: 201 DFNNDKYVDDMHLAIEELIRVSGYARLAEAFYYAKYLPSHKKAVREVEEAQRRVQNLVSP 260
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+ N P N++LHFL SQ Y DE+I + E + LGVDST+ T WAL+FL+ E +QEK
Sbjct: 261 FLSLNPPTNTYLHFLRSQKYDDEVIIFAIFEAYLLGVDSTSLTTAWALAFLIREPNVQEK 320
Query: 475 LYREINN--RTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRR 302
LY+E+ + +R +KV D+ +L YLQAV+KET+RMKPIAPLA+PH A +DT+ G++
Sbjct: 321 LYQELESFASKNDRRILKVEDINKLQYLQAVIKETMRMKPIAPLAIPHKACRDTSLMGKK 380
Query: 301 IVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMR 122
I KGT+VMVN++A+HH+ NVF P+KFMPERF+K D + + ME SL+PFSAGMR
Sbjct: 381 IDKGTRVMVNIFALHHNKNVFNDPFKFMPERFMK---VDSQDANGKAMEQSLLPFSAGMR 437
Query: 121 ICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
IC G+EL K +FALA++ FKW CV++G LPD+S+ +
Sbjct: 438 ICAGMELGKLQFSFALANLAYAFKWSCVADGVLPDMSDQL 477
>gb|AHF65153.1| stylopine synthase, partial [synthetic construct]
Length = 511
Score = 291 bits (745), Expect = 2e-76
Identities = 145/283 (51%), Positives = 199/283 (70%), Gaps = 5/283 (1%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF ++ V + A++E++R+SG A LA+AF AKYLPS +K +RD+ RV L++P
Sbjct: 215 DFDDDKYVDDMHHAIEELIRVSGYAQLAEAFYYAKYLPSHKKAVRDVEEANQRVKKLVRP 274
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+ N P N++LHFL SQ Y +E+I + E + LGVDST+ T WAL+FL+ E +Q+K
Sbjct: 275 FLSLNPPTNTYLHFLNSQKYDEEVIIFAIFEAYLLGVDSTSLTTAWALAFLIREPVVQDK 334
Query: 475 LYREINNRTGGQ--RPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRR 302
LY+E+ N T VKV DL +L YLQAV+KET+RMKPIAPLA+PH A +DT+ KG +
Sbjct: 335 LYQELKNLTAKNDCEIVKVEDLNKLQYLQAVLKETMRMKPIAPLAIPHKACRDTSLKGNK 394
Query: 301 IVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKD---VNSDGRFGDINTMESSLIPFSA 131
I +GT+VMVN+YA+HH+ V+ P+KFMPERFL+ VN G ME SL+PFSA
Sbjct: 395 IDQGTQVMVNIYALHHNEKVWKEPFKFMPERFLQTQDVVNGKG-------MEQSLLPFSA 447
Query: 130 GMRICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
GMRIC G+EL K +F+LA++VN F+W CVS+G LPD+S+ +
Sbjct: 448 GMRICAGMELGKLQFSFSLANLVNAFEWSCVSDGVLPDMSDQL 490
>gb|ADB89214.1| stylopine synthase [Papaver somniferum]
Length = 494
Score = 291 bits (745), Expect = 2e-76
Identities = 145/283 (51%), Positives = 199/283 (70%), Gaps = 5/283 (1%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF ++ V + A++E++R+SG A LA+AF AKYLPS +K +RD+ RV L++P
Sbjct: 201 DFDDDKYVDDMHHAIEELIRVSGYAQLAEAFYYAKYLPSHKKAVRDVEEANQRVKKLVRP 260
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+ N P N++LHFL SQ Y +E+I + E + LGVDST+ T WAL+FL+ E +Q+K
Sbjct: 261 FLSLNPPTNTYLHFLNSQKYDEEVIIFAIFEAYLLGVDSTSLTTAWALAFLIREPVVQDK 320
Query: 475 LYREINNRTGGQ--RPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRR 302
LY+E+ N T VKV DL +L YLQAV+KET+RMKPIAPLA+PH A +DT+ KG +
Sbjct: 321 LYQELKNLTAKNDCEIVKVEDLNKLQYLQAVLKETMRMKPIAPLAIPHKACRDTSLKGNK 380
Query: 301 IVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKD---VNSDGRFGDINTMESSLIPFSA 131
I +GT+VMVN+YA+HH+ V+ P+KFMPERFL+ VN G ME SL+PFSA
Sbjct: 381 IDQGTQVMVNIYALHHNEKVWKEPFKFMPERFLQTQDVVNGKG-------MEQSLLPFSA 433
Query: 130 GMRICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
GMRIC G+EL K +F+LA++VN F+W CVS+G LPD+S+ +
Sbjct: 434 GMRICAGMELGKLQFSFSLANLVNAFEWSCVSDGVLPDMSDQL 476
>gb|AGL76711.1| (S)-canadine synthase, partial [Coptis chinensis]
Length = 491
Score = 291 bits (744), Expect = 3e-76
Identities = 139/278 (50%), Positives = 195/278 (70%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
+F + + + +++++RISG A LA+AF AKYLPS +K R+ + + RV L++P
Sbjct: 198 NFDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCRVQELVRP 257
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+ SN P NS+L+FL SQ++ +E+I + E++ LGVDST++T WAL++L+ EQ QEK
Sbjct: 258 LLSSNPPTNSYLYFLLSQNFEEEVIIFCIFELYLLGVDSTSSTTTWALAYLIREQGAQEK 317
Query: 475 LYREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIV 296
LY++I G VK+ D+ +L YLQ V+KET+RMKPIAPLA+PH AK+TT G ++
Sbjct: 318 LYQDIRMTLGDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLAIPHKTAKETTLMGTKVA 377
Query: 295 KGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMRIC 116
KGT++MVNLYA+HH+ N++P PYKFMPERFL+ ME S +PFSAGMRIC
Sbjct: 378 KGTRIMVNLYALHHNQNIWPDPYKFMPERFLEGATGTAY---NKAMEQSFLPFSAGMRIC 434
Query: 115 GGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
G++L K AFALA++VN FKW CV EGKLPD+ E +
Sbjct: 435 AGMDLGKLQFAFALANLVNAFKWSCVEEGKLPDMGEEL 472
>dbj|BAG75116.1| cytochrome P450 [Eschscholzia californica]
Length = 495
Score = 290 bits (743), Expect = 4e-76
Identities = 145/280 (51%), Positives = 199/280 (71%), Gaps = 2/280 (0%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF+++ V + A++E++R+SG A LA+AF AKYLPS +K IR T+ V NL++P
Sbjct: 201 DFNDDKYVEDLHQAIEELIRMSGYAQLAEAFYYAKYLPSHKKAIRHAELTKQIVTNLVRP 260
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+ N P NS+LHFL SQ Y +E++ + E + LGVDST++T WAL++LV E +Q++
Sbjct: 261 FLSLNPPTNSYLHFLQSQKYDEEMVIFAIFETYLLGVDSTSSTTAWALAYLVREPNVQDR 320
Query: 475 LYREINN--RTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRR 302
LY E+NN ++ +KV + +L YLQAV KET+RMKPIAPLA+PH A +DT+ G +
Sbjct: 321 LYNELNNYANYNDRKILKVEVINKLQYLQAVTKETMRMKPIAPLAIPHKACRDTSLMGTK 380
Query: 301 IVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMR 122
I KGTKVMVNL+A+HH+ NVF P+KFMPERFLK N D ++ ME SL+PFSAGMR
Sbjct: 381 IDKGTKVMVNLHALHHNENVFTDPFKFMPERFLKVNNED---VNMKAMEQSLLPFSAGMR 437
Query: 121 ICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
IC G+EL K +FALA++V FKW CV +G LPD+S+ +
Sbjct: 438 ICAGMELGKLQFSFALANLVYAFKWSCVDDGVLPDMSDEL 477
>sp|Q50LH4.1|C7193_ESCCA RecName: Full=(S)-stylopine synthase 2; Short=STS; AltName:
Full=Cytochrome P450 719A3; Short=EcCYP719A3;
Short=EcCYPA gi|63108254|dbj|BAD98249.1| stylopine
synthase [Eschscholzia californica]
Length = 495
Score = 290 bits (743), Expect = 4e-76
Identities = 143/280 (51%), Positives = 201/280 (71%), Gaps = 2/280 (0%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF + V + A++E++RISG A LA+AF AKYLPS RK +R + + V NLI+P
Sbjct: 201 DFEEDKYVEDMHHAIEELIRISGYARLAEAFYYAKYLPSHRKAVRYVEELKQIVKNLIRP 260
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+ N P N++LHFL SQ+Y +E++ + E + LGVDST++T WAL++LV E +Q++
Sbjct: 261 FLSVNPPTNTYLHFLRSQNYDEEVVIFAIFETYLLGVDSTSSTTAWALAYLVREPSVQDR 320
Query: 475 LYREINN--RTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRR 302
L++E+++ + ++ +KV D+ +L YLQAV+KET+RMKPIAPLA+PH A KDT+ G +
Sbjct: 321 LHQELDHFAKQNDRKILKVEDMNKLQYLQAVIKETMRMKPIAPLAIPHKACKDTSLMGNK 380
Query: 301 IVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMR 122
I KGT+VMVNLYA+HH+ NVF P+KFMPERFLK N D + ME SL+PFSAGMR
Sbjct: 381 INKGTRVMVNLYALHHNKNVFNDPFKFMPERFLKVDNQDAKG---KAMEQSLLPFSAGMR 437
Query: 121 ICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
IC G+EL K +FALA+++ FKW CV +G LPD+S+ +
Sbjct: 438 ICAGMELGKLQFSFALANLIFAFKWSCVDDGVLPDMSDEL 477
>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
Full=(S)-tetrahydrocolumbamine oxidase
(methylenedioxy-bridge-forming); AltName: Full=Cytochrome
P450 719A1 gi|15822820|dbj|BAB68769.1| methylenedioxy
bridge-forming enzyme [Coptis japonica]
Length = 491
Score = 288 bits (737), Expect = 2e-75
Identities = 140/279 (50%), Positives = 195/279 (69%), Gaps = 2/279 (0%)
Frame = -1
Query: 832 FSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQPH 653
F + + + +++++RISG A LA+AF AKYLPS +K R+ + + RV L++P
Sbjct: 199 FDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCRVEELVRPL 258
Query: 652 IVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEKL 473
+ S P NS+L+FL SQ++ +E+I + E++ LGVDST++T WAL++L+ EQ QEKL
Sbjct: 259 LSSKPPTNSYLYFLLSQNFEEEVIIFCIFELYLLGVDSTSSTTTWALAYLIREQGAQEKL 318
Query: 472 YREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIVK 293
Y++I G VK+ D+ +L YLQ V+KET+RMKPIAPLA+PH AK+TT G ++ K
Sbjct: 319 YQDIRMTLGDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLAIPHKTAKETTLMGTKVAK 378
Query: 292 GTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDI--NTMESSLIPFSAGMRI 119
GT++MVNLYA+HH+ N++P PYKFMPERFL +G G ME S +PFSAGMRI
Sbjct: 379 GTRIMVNLYALHHNQNIWPDPYKFMPERFL-----EGETGTAYNKAMEQSFLPFSAGMRI 433
Query: 118 CGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
C G++L K AFALA++VN FKW CV EGKLPD+ E +
Sbjct: 434 CAGMDLGKLQFAFALANLVNAFKWSCVEEGKLPDMGEEL 472
>gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum]
Length = 488
Score = 283 bits (724), Expect = 6e-74
Identities = 140/280 (50%), Positives = 195/280 (69%), Gaps = 2/280 (0%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF ++ V + D ++ ++RISG A LA+ F AKYLPS ++ + + RV L++P
Sbjct: 199 DFDDDKYVEDMHDVIEFLIRISGYAQLAEVFYYAKYLPSHKRAVTGAEEAKRRVIALVRP 258
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+ SN N++LHFL SQ Y +E+I + E + LGVDST++T WAL+FL+ E +QEK
Sbjct: 259 FLQSNPATNTYLHFLKSQLYPEEVIIFAIFEAYLLGVDSTSSTTAWALAFLIREPSVQEK 318
Query: 475 LYREINNRT--GGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRR 302
LY+E+ N T + +KV D+ +LPYLQAV+KET+RMKPIAPLA+PH A KDT+ G++
Sbjct: 319 LYQELKNFTANNNRTMLKVEDVNKLPYLQAVVKETMRMKPIAPLAIPHKACKDTSLMGKK 378
Query: 301 IVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMR 122
+ KGTKVMVN++A+HH V+ PYKFMPERFL+ + ME SL+PFSAGMR
Sbjct: 379 VDKGTKVMVNIHALHHTEKVWKEPYKFMPERFLQKHD--------KAMEQSLLPFSAGMR 430
Query: 121 ICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
IC G+EL K +F+LA++VN FKW CVS+G LPD+S+ +
Sbjct: 431 ICAGMELGKLQFSFSLANLVNAFKWSCVSDGVLPDMSDLL 470
>dbj|BAG75114.1| cytochrome P450 [Eschscholzia californica]
Length = 495
Score = 283 bits (723), Expect = 7e-74
Identities = 138/280 (49%), Positives = 193/280 (68%), Gaps = 2/280 (0%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF + V + +DE++R+SG A LA+AF A+YLP+ +K + T+ V +L++P
Sbjct: 202 DFDEDTYVEKLHHTIDELIRMSGYAQLAEAFYYARYLPNHKKAVNHAAKTKQIVTHLMRP 261
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+ N P NS+LHFL SQ+Y +E+I + E++ LGVDST++T WAL++LV E +QE
Sbjct: 262 FLSLNPPTNSYLHFLQSQNYDEELIIFSIFEVYLLGVDSTSSTTAWALAYLVREPNVQET 321
Query: 475 LYREINN--RTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRR 302
LY+E++N + ++ +KV D+ +L YLQAV KET+RMKPIAPLA+PH A++DTT G++
Sbjct: 322 LYQELDNFAKQNDRKILKVEDINKLQYLQAVTKETMRMKPIAPLAIPHKASRDTTLMGKK 381
Query: 301 IVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMR 122
+ KGTKVMVNL+A+HH+ NVF PYKFMPERFLK ME S +PFSAGMR
Sbjct: 382 VEKGTKVMVNLHALHHNENVFNDPYKFMPERFLKSYQG----AKAKAMEQSYLPFSAGMR 437
Query: 121 ICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
ICGG+E+ K FALA++ FKW C G LPD+S+ +
Sbjct: 438 ICGGMEVGKLQFGFALANLAYAFKWSCAVNGVLPDMSDEL 477
>gb|AFB74615.1| cytochrome P450 [Papaver somniferum]
Length = 486
Score = 280 bits (716), Expect = 5e-73
Identities = 138/280 (49%), Positives = 194/280 (69%), Gaps = 2/280 (0%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
DF ++ V + D ++ ++RISG A LA+ F AKYLP ++ + + RV L++P
Sbjct: 197 DFDDDKYVEDMHDVIEFLIRISGYAQLAEVFYYAKYLPGHKRAVTGAEEAKRRVIALVRP 256
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+ SN N++LHFL SQ Y +E+I + E + LGVDST++T WAL+FL+ E +QEK
Sbjct: 257 FLQSNPATNTYLHFLKSQLYPEEVIIFAIFEAYLLGVDSTSSTTAWALAFLIREPSVQEK 316
Query: 475 LYREINNRT--GGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRR 302
LY+E+ N T + +KV D+ +LPYLQAV+KET+RMKPIAPLA+PH A KDT+ G++
Sbjct: 317 LYQELKNFTANNNRTMLKVEDVNKLPYLQAVVKETMRMKPIAPLAIPHKACKDTSLMGKK 376
Query: 301 IVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMR 122
+ KGTKVMVN++A+HH V+ PYKF+PERFL+ + ME SL+PFSAGMR
Sbjct: 377 VDKGTKVMVNIHALHHTEKVWKEPYKFIPERFLQKHD--------KAMEQSLLPFSAGMR 428
Query: 121 ICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
IC G+EL K +F+LA++VN FKW CVS+G LPD+S+ +
Sbjct: 429 ICAGMELGKLQFSFSLANLVNAFKWSCVSDGVLPDMSDLL 468
>dbj|BAG75115.1| cytochrome P450 [Eschscholzia californica]
Length = 494
Score = 279 bits (714), Expect = 8e-73
Identities = 137/280 (48%), Positives = 195/280 (69%), Gaps = 2/280 (0%)
Frame = -1
Query: 835 DFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQP 656
+F+++ V + + +DE++++S A LA+AF AKYLPS RK +R T+ V LI+P
Sbjct: 202 NFNDDQYVEDMHNTIDELIQMSSYAQLAEAFYYAKYLPSHRKAVRHFEQTKQIVKKLIRP 261
Query: 655 HIVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEK 476
+I + P N++LHFL SQ+Y +E++ + E + LGVDST++T WAL+FL+ E +QEK
Sbjct: 262 YISVSPPPNTYLHFLQSQNYDEEVVIFAIFEAYLLGVDSTSSTTTWALAFLIREPNVQEK 321
Query: 475 LYREINN--RTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRR 302
LY+E+ N + +KV D+ +L YLQAV KET+RMKP+APLA+PH A ++T+ G
Sbjct: 322 LYQELKNFAEQNDREIMKVEDINKLQYLQAVTKETMRMKPVAPLAIPHKACRETSLMGMP 381
Query: 301 IVKGTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDINTMESSLIPFSAGMR 122
I KGTKVMVN++++HH+ NVF P+ FMPERFLK N ++ ME S +PFSAGMR
Sbjct: 382 IDKGTKVMVNIHSLHHNENVFSDPFTFMPERFLKVDNQ-----NVKAMEQSYLPFSAGMR 436
Query: 121 ICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
IC G+EL K FALA++V FKW CV++G LPD+SE +
Sbjct: 437 ICAGIELGKLHFGFALANLVYAFKWSCVADGVLPDMSEEL 476
>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 508
Score = 272 bits (696), Expect = 1e-70
Identities = 136/279 (48%), Positives = 192/279 (68%), Gaps = 2/279 (0%)
Frame = -1
Query: 832 FSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRKNIRDMYATRDRVYNLIQPH 653
F++ + + +++ +R S SL ++F AKYLPSQRK R+ + R+ L++P
Sbjct: 215 FNDSKFIESMLYEIEDFIRTSSYTSLTNSFYYAKYLPSQRKATREAKLGKYRIEELVRPL 274
Query: 652 IVSNLPANSFLHFLTSQDYSDEIIYSMVLEIFGLGVDSTAATAVWALSFLVCEQEIQEKL 473
+ S P N++L+FL SQ+ S+E+ + E+F LGVDS ++T WAL++++ EQE QEKL
Sbjct: 275 LSSKPPTNAYLYFLLSQNLSEEVTIFCIFELFLLGVDSASSTTTWALAYMIREQEAQEKL 334
Query: 472 YREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIVK 293
Y++I G VK+ D+ +L YLQ+V+KET+RMKPIAPLA+PH A KDTT G +IVK
Sbjct: 335 YQDIRMTIGDVDLVKIEDVNKLKYLQSVVKETMRMKPIAPLALPHNAGKDTTLMGTKIVK 394
Query: 292 GTKVMVNLYAIHHDPNVFPAPYKFMPERFLKDVNSDGRFGDI--NTMESSLIPFSAGMRI 119
GT+VMVNLYA+HH +V+ P KFMPER+L+ G G + ME SL+PF AGMRI
Sbjct: 395 GTRVMVNLYALHHYEDVWLEPSKFMPERYLQ-----GEDGSVTHKAMEQSLLPFGAGMRI 449
Query: 118 CGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAI 2
C G++L K VAF LA++VN FKW CV +GKLPD++E +
Sbjct: 450 CAGMDLGKLQVAFVLANLVNAFKWSCVEDGKLPDMTEEL 488