BLASTX nr result
ID: Papaver27_contig00010153
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver27_contig00010153
(900 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002276931.1| PREDICTED: uncharacterized protein LOC100265... 189 2e-45
gb|EXB37387.1| 30S ribosomal protein S1 [Morus notabilis] 187 6e-45
emb|CBI34709.3| unnamed protein product [Vitis vinifera] 187 6e-45
emb|CAN68047.1| hypothetical protein VITISV_017724 [Vitis vinifera] 186 8e-45
ref|XP_006584710.1| PREDICTED: uncharacterized protein LOC100809... 174 3e-41
ref|XP_003532406.1| PREDICTED: uncharacterized protein LOC100809... 174 3e-41
ref|XP_004503759.1| PREDICTED: bromodomain testis-specific prote... 174 4e-41
ref|XP_007040177.1| Nucleic acid-binding, putative isoform 3 [Th... 171 3e-40
ref|XP_007040176.1| Nucleic acid-binding, putative isoform 2 [Th... 171 3e-40
ref|XP_007040175.1| Nucleic acid-binding, OB-fold-like protein, ... 171 3e-40
ref|XP_007210897.1| hypothetical protein PRUPE_ppa001627mg [Prun... 168 2e-39
ref|XP_007210896.1| hypothetical protein PRUPE_ppa001627mg [Prun... 168 2e-39
ref|XP_006385758.1| hypothetical protein POPTR_0003s12430g [Popu... 168 3e-39
ref|XP_002304482.2| S1 RNA-binding domain-containing family prot... 168 3e-39
ref|XP_006352282.1| PREDICTED: uncharacterized protein LOC102596... 167 4e-39
ref|XP_004244605.1| PREDICTED: uncharacterized protein LOC101250... 167 7e-39
ref|XP_007160042.1| hypothetical protein PHAVU_002G287600g [Phas... 164 4e-38
ref|XP_006417193.1| hypothetical protein EUTSA_v10006889mg [Eutr... 164 4e-38
ref|XP_002509767.1| conserved hypothetical protein [Ricinus comm... 164 6e-38
ref|XP_006417194.1| hypothetical protein EUTSA_v10006889mg [Eutr... 160 5e-37
>ref|XP_002276931.1| PREDICTED: uncharacterized protein LOC100265091 [Vitis vinifera]
Length = 773
Score = 189 bits (479), Expect = 2e-45
Identities = 123/326 (37%), Positives = 175/326 (53%), Gaps = 42/326 (12%)
Frame = +3
Query: 48 DFNNLRKPEAAVFNTTDDDNEHEKKNSADFNSL------------------------RKP 155
D LRKPE + D +NE ++++S D +L RKP
Sbjct: 200 DMTLLRKPEKL---SADAENETKQESSDDARALATDDTELKLQEEGTDDKINDVMLMRKP 256
Query: 156 KAAVVSTTDDDN-EHAKKNSADLT-----RLSSGGFENSDDFNSLSSVDYNSILQKPNHA 317
+ ++S D+ EH+ A ++ SSG E + NS+++ S+ + +
Sbjct: 257 EPTIISANLDEKLEHSGDAEAKISIGIEEGSSSGSSEYTGAANSMNNDIEESLETRDDSF 316
Query: 318 PFA-----------KPLERSDVV-LSVMDDVTAVKQPSDTNSSNSAIESTVEAALQGKPQ 461
+PLE SD++ + TA +PS+ S + + ++EAALQGKP+
Sbjct: 317 SMGPELVDNSIIGLQPLEHSDIIDMGPAKVETAASEPSNRKSVDPKGKLSMEAALQGKPK 376
Query: 462 RLNPSAKQSSVSLKEETNRVSDNIFSVSVDIGDFIAAAPVKDQEDADWTRAGNLLKTEER 641
RL S K+ S + ET + + SV++ +F+A + +K ED DW+RA +L+KT R
Sbjct: 377 RLEQSVKEMSNLSQPETVLANPESYGNSVELENFLATSSLKGHEDTDWSRAEDLVKTGGR 436
Query: 642 EEVELISCSTRGFVASFGSLVGFLPYRNLGSKWKFLTFESWLRKKGLDLSMYKQNASIIG 821
EEVELIS STRGFV SFGSL+GFLPYRNL +KWKFL FESWLR+KGLD SMY+QN I+G
Sbjct: 437 EEVELISSSTRGFVVSFGSLIGFLPYRNLAAKWKFLAFESWLRRKGLDPSMYRQNLGIVG 496
Query: 822 GYEAQTKSLPLDTNPSPATVGKFGGE 899
+E P D NP P + GE
Sbjct: 497 SHEVANNPSP-DANPGPEIHKQLEGE 521
>gb|EXB37387.1| 30S ribosomal protein S1 [Morus notabilis]
Length = 1032
Score = 187 bits (474), Expect = 6e-45
Identities = 131/303 (43%), Positives = 164/303 (54%), Gaps = 15/303 (4%)
Frame = +3
Query: 36 ENADDFNNLRKPEAAVFNTTDDDNEHEKKNSADFNSLRKPKAAVVSTTDDDNEHAKKN-- 209
E D LRKPE N + D E K + +D L KPKA + T + + +
Sbjct: 219 EEFSDMTLLRKPEPMSVNESSDAKEERKDDVSDVTLLNKPKAIDIKTQIESSAEQVASVD 278
Query: 210 -------SADLT----RLSS-GGFENSDDFNSLSSVDYNSILQKPNHAPFAKPLERSDVV 353
SA +T RLS G N N SV+ S +KP +P VV
Sbjct: 279 YVESDLVSATVTNGSKRLSELTGSANVTPSNHEESVEI-SFTEKPTRLQPDEP----SVV 333
Query: 354 LSVMDDVTAVKQPSDTNSSNSAIESTVEAALQGKPQRLNPSAKQSSVSLKEETNRV-SDN 530
S + + K T+S S+ +VEAAL GKP+RL+ S K +SVS + S+N
Sbjct: 334 SSGEEKIE--KGLPHTSSVFSSANLSVEAALVGKPKRLDQSVKGTSVSPVSAKKPIGSEN 391
Query: 531 IFSVSVDIGDFIAAAPVKDQEDADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGF 710
+ + +PV+ EDADWTRA LLKT +R EVELISCSTRGFV SFGSL+GF
Sbjct: 392 V----------LVKSPVEGIEDADWTRAEGLLKTGDRGEVELISCSTRGFVVSFGSLIGF 441
Query: 711 LPYRNLGSKWKFLTFESWLRKKGLDLSMYKQNASIIGGYEAQTKSLPLDTNPSPATVGKF 890
LPYRNL SKWKFL FESWLR+KGLD S+Y+QN IIG YEA T S L ++ P +
Sbjct: 442 LPYRNLSSKWKFLAFESWLRRKGLDPSLYRQNLGIIGNYEAATNSSLLRSSIDPKIDIEV 501
Query: 891 GGE 899
GGE
Sbjct: 502 GGE 504
>emb|CBI34709.3| unnamed protein product [Vitis vinifera]
Length = 707
Score = 187 bits (474), Expect = 6e-45
Identities = 125/296 (42%), Positives = 164/296 (55%), Gaps = 16/296 (5%)
Frame = +3
Query: 60 LRKPEAAVFNTTDDDNEHEKKNSADFNSLRKPKAAVVS--TTDDDNEHAKKNSADLTRLS 233
LRKP VFN D D++ + SL+ K A S T E K S + S
Sbjct: 164 LRKP--TVFNEDDVDSKPSRLRMKPNLSLKMKKEAKFSDMTLLRKPEKLTKISIGIEEGS 221
Query: 234 SGGFE---------NSDDFNSLSSVDYNSILQKP----NHAPFAKPLERSDVV-LSVMDD 371
S G N+D SL + D +S P N +PLE SD++ +
Sbjct: 222 SSGSSEYTGAANSMNNDIEESLETRD-DSFSMGPELVDNSIIGLQPLEHSDIIDMGPAKV 280
Query: 372 VTAVKQPSDTNSSNSAIESTVEAALQGKPQRLNPSAKQSSVSLKEETNRVSDNIFSVSVD 551
TA +PS+ S + + ++EAALQGKP+RL S K+ S + ET + + SV+
Sbjct: 281 ETAASEPSNRKSVDPKGKLSMEAALQGKPKRLEQSVKEMSNLSQPETVLANPESYGNSVE 340
Query: 552 IGDFIAAAPVKDQEDADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNLG 731
+ +F+A + +K ED DW+RA +L+KT REEVELIS STRGFV SFGSL+GFLPYRNL
Sbjct: 341 LENFLATSSLKGHEDTDWSRAEDLVKTGGREEVELISSSTRGFVVSFGSLIGFLPYRNLA 400
Query: 732 SKWKFLTFESWLRKKGLDLSMYKQNASIIGGYEAQTKSLPLDTNPSPATVGKFGGE 899
+KWKFL FESWLR+KGLD SMY+QN I+G +E P D NP P + GE
Sbjct: 401 AKWKFLAFESWLRRKGLDPSMYRQNLGIVGSHEVANNPSP-DANPGPEIHKQLEGE 455
>emb|CAN68047.1| hypothetical protein VITISV_017724 [Vitis vinifera]
Length = 768
Score = 186 bits (473), Expect = 8e-45
Identities = 120/317 (37%), Positives = 171/317 (53%), Gaps = 42/317 (13%)
Frame = +3
Query: 48 DFNNLRKPEAAVFNTTDDDNEHEKKNSADFNSL------------------------RKP 155
D LRKPE + D +NE ++++S D +L RKP
Sbjct: 200 DMTLLRKPEKL---SADAENETKQESSDDARALATDDTELKLQEEGTDDKINDVMLMRKP 256
Query: 156 KAAVVSTTDDDN-EHAKKNSADLT-----RLSSGGFENSDDFNSLSSVDYNSILQKPNHA 317
+ ++S D+ EH+ A ++ SSG E + NS+++ S+ + +
Sbjct: 257 EPTIISANLDEKLEHSGDAEAKISIGIEXGSSSGSSEYTGAANSMNNDIEESLETRDDSF 316
Query: 318 PFA-----------KPLERSDVV-LSVMDDVTAVKQPSDTNSSNSAIESTVEAALQGKPQ 461
+PLE SD++ + T +PS+ S + + ++EAALQGKP+
Sbjct: 317 SMGPELVDNSIIGLQPLEHSDIIDMGPAKVETXASEPSNXKSVDPKGKLSMEAALQGKPK 376
Query: 462 RLNPSAKQSSVSLKEETNRVSDNIFSVSVDIGDFIAAAPVKDQEDADWTRAGNLLKTEER 641
RL S K+ S + ET + + SV++ +F+A + +K ED DW+RA +L+KT R
Sbjct: 377 RLEQSVKEMSXLSQPETVLANPESYGNSVELENFLATSSLKGHEDTDWSRAEDLVKTGGR 436
Query: 642 EEVELISCSTRGFVASFGSLVGFLPYRNLGSKWKFLTFESWLRKKGLDLSMYKQNASIIG 821
EEVELIS STRGFV SFGSL+GFLPYRNL +KWKFL FESWLR+KGLD SMY+QN I+G
Sbjct: 437 EEVELISSSTRGFVVSFGSLIGFLPYRNLAAKWKFLAFESWLRRKGLDPSMYRQNLGIVG 496
Query: 822 GYEAQTKSLPLDTNPSP 872
+E P D NP P
Sbjct: 497 SHEVANNPSP-DANPGP 512
>ref|XP_006584710.1| PREDICTED: uncharacterized protein LOC100809060 isoform X2 [Glycine
max]
Length = 588
Score = 174 bits (442), Expect = 3e-41
Identities = 106/277 (38%), Positives = 158/277 (57%), Gaps = 16/277 (5%)
Frame = +3
Query: 105 NEHEKKNSADFNSLRKPKAAVVSTTDDDNEHAKKNSADLTRLSSGGFENSDDFNSLSSVD 284
+E K +D LRKP+AA+ + ++ + L E SD+ +
Sbjct: 199 DEQVKARFSDMTLLRKPEAAIQEPSSSVDDQGNYDG----ELKMWNGELSDEIGGFT--- 251
Query: 285 YNSILQKPNHAPFAKPLE---RSDVVLSVMDDVTAVKQPSDTN-------------SSNS 416
+L++P H P + E R + ++VM ++Q + S +S
Sbjct: 252 ---LLERP-HKPSGEKEESGEREMLEVNVMIPNDGLEQHEERQLEFHEESTDLGQLSDDS 307
Query: 417 AIESTVEAALQGKPQRLNPSAKQSSVSLKEETNRVSDNIFSVSVDIGDFIAAAPVKDQED 596
+E +VEAALQ KP+RL+ KQ+S + EE ++ + D+G + + ++ ED
Sbjct: 308 RVELSVEAALQAKPKRLDQYVKQASKLVGEEGASLNIGARTNKDDLGKVVDMSDFQESED 367
Query: 597 ADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNLGSKWKFLTFESWLRKK 776
ADWTRA +L+KT +RE+VEL+SC+T+GF+ SFGSLVGFLPYRNL SKWKFL FESWL++K
Sbjct: 368 ADWTRAQDLIKTGDREDVELVSCNTKGFIVSFGSLVGFLPYRNLASKWKFLAFESWLKQK 427
Query: 777 GLDLSMYKQNASIIGGYEAQTKSLPLDTNPSPATVGK 887
GLD S+YKQN+ I ++A+ K+L D+ PS GK
Sbjct: 428 GLDPSIYKQNSGTITSFDAEIKNLSPDSPPSLEIDGK 464
>ref|XP_003532406.1| PREDICTED: uncharacterized protein LOC100809060 isoform X1 [Glycine
max]
Length = 722
Score = 174 bits (442), Expect = 3e-41
Identities = 106/277 (38%), Positives = 158/277 (57%), Gaps = 16/277 (5%)
Frame = +3
Query: 105 NEHEKKNSADFNSLRKPKAAVVSTTDDDNEHAKKNSADLTRLSSGGFENSDDFNSLSSVD 284
+E K +D LRKP+AA+ + ++ + L E SD+ +
Sbjct: 199 DEQVKARFSDMTLLRKPEAAIQEPSSSVDDQGNYDG----ELKMWNGELSDEIGGFT--- 251
Query: 285 YNSILQKPNHAPFAKPLE---RSDVVLSVMDDVTAVKQPSDTN-------------SSNS 416
+L++P H P + E R + ++VM ++Q + S +S
Sbjct: 252 ---LLERP-HKPSGEKEESGEREMLEVNVMIPNDGLEQHEERQLEFHEESTDLGQLSDDS 307
Query: 417 AIESTVEAALQGKPQRLNPSAKQSSVSLKEETNRVSDNIFSVSVDIGDFIAAAPVKDQED 596
+E +VEAALQ KP+RL+ KQ+S + EE ++ + D+G + + ++ ED
Sbjct: 308 RVELSVEAALQAKPKRLDQYVKQASKLVGEEGASLNIGARTNKDDLGKVVDMSDFQESED 367
Query: 597 ADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNLGSKWKFLTFESWLRKK 776
ADWTRA +L+KT +RE+VEL+SC+T+GF+ SFGSLVGFLPYRNL SKWKFL FESWL++K
Sbjct: 368 ADWTRAQDLIKTGDREDVELVSCNTKGFIVSFGSLVGFLPYRNLASKWKFLAFESWLKQK 427
Query: 777 GLDLSMYKQNASIIGGYEAQTKSLPLDTNPSPATVGK 887
GLD S+YKQN+ I ++A+ K+L D+ PS GK
Sbjct: 428 GLDPSIYKQNSGTITSFDAEIKNLSPDSPPSLEIDGK 464
>ref|XP_004503759.1| PREDICTED: bromodomain testis-specific protein-like [Cicer arietinum]
Length = 809
Score = 174 bits (441), Expect = 4e-41
Identities = 105/293 (35%), Positives = 155/293 (52%), Gaps = 8/293 (2%)
Frame = +3
Query: 36 ENADDFNNL---RKPEAAVFNTTDDDNEHEKKNSADFNSLRKPKAAVVSTTDDDNEHAKK 206
E +DD NL +P N + + K + L +P V+ + +
Sbjct: 271 EPSDDVGNLTLLEQPHRPNVNKQQEQFQEPKDEVGNLTLLEQPHRPSVNKQQELFQEPTD 330
Query: 207 NSADLTRLS-----SGGFENSDDFNSLSSVDYNSILQKPNHAPFAKPLERSDVVLSVMDD 371
LT L SG E + F V N + + + V +
Sbjct: 331 EGVKLTLLEQPHRPSGKEEEEEQFGDARVVVPNDGSE-----------QHEQIQSEVHQE 379
Query: 372 VTAVKQPSDTNSSNSAIESTVEAALQGKPQRLNPSAKQSSVSLKEETNRVSDNIFSVSVD 551
+ + Q SD NS +S E +VEAA+QGKP+RL+ K++S S++EET + S +
Sbjct: 380 LINLNQLSDLNSVDSKTELSVEAAIQGKPKRLDRYVKKTSQSVEEETASLDPGGRENSEE 439
Query: 552 IGDFIAAAPVKDQEDADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNLG 731
G+ + ++++EDADWT+A L+KT +R +VEL+SCST+GFV SFG+LVGFLPYRNL
Sbjct: 440 SGNLVDVNDIQEREDADWTKAEGLIKTGDRADVELVSCSTKGFVVSFGTLVGFLPYRNLL 499
Query: 732 SKWKFLTFESWLRKKGLDLSMYKQNASIIGGYEAQTKSLPLDTNPSPATVGKF 890
S+WKFL FESWLR+KGLD S+YKQ + + Y+A+ ++ D+ GKF
Sbjct: 500 SRWKFLAFESWLRQKGLDPSLYKQTLATVTNYDAENNNISTDSPSHIENDGKF 552
>ref|XP_007040177.1| Nucleic acid-binding, putative isoform 3 [Theobroma cacao]
gi|508777422|gb|EOY24678.1| Nucleic acid-binding,
putative isoform 3 [Theobroma cacao]
Length = 592
Score = 171 bits (434), Expect = 3e-40
Identities = 116/294 (39%), Positives = 160/294 (54%), Gaps = 14/294 (4%)
Frame = +3
Query: 60 LRKPEAAVFNTTDDDN---------EHEKK---NSADFNSLRKPKAAVVSTTDDDNEHAK 203
LRKPE +T+ D E EK+ DF L K + ++ + + E +
Sbjct: 217 LRKPEPMSVDTSLDKKQDSEGSVGLEKEKEVEDRIGDFTILEKSEQSIHANIREMQELFE 276
Query: 204 KNSADLTRLSSGGFENSDDFNSLSSVDYNSILQ--KPNHAPFAKPLERSDVVLSVMDDVT 377
+ RL E + N L++ N+ + + H+ K ER D +S M V
Sbjct: 277 DLEIEGQRL-----EAEIEANMLANATENTSQESLEAGHSSIPKKPERKDDSISGMQPVE 331
Query: 378 AVKQPSDTNSSNSAIESTVEAALQGKPQRLNPSAKQSSVSLKEETNRVSDNIFSVSVDIG 557
+ S SS ++EAALQGKP+RL+ S K++S S + +T ++ D G
Sbjct: 332 LSNRVSTEESSTGP---SIEAALQGKPKRLDQSVKETSNSSRAQTVPINPE------DYG 382
Query: 558 DFIAAAPVKDQEDADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNLGSK 737
D + +P QED+DWTR +LLKT ER EVELIS STRGFV SFGSL+GFLPYRNL +K
Sbjct: 383 DLPSVSP---QEDSDWTRVEHLLKTGERAEVELISSSTRGFVVSFGSLIGFLPYRNLAAK 439
Query: 738 WKFLTFESWLRKKGLDLSMYKQNASIIGGYEAQTKSLPLDTNPSPATVGKFGGE 899
WKFL FESWLR+KGLDL+ YKQN +IG + +K+ L +N +F G+
Sbjct: 440 WKFLAFESWLRQKGLDLAAYKQNLGVIGSSDIMSKNSSLVSNSDMENNQQFEGK 493
>ref|XP_007040176.1| Nucleic acid-binding, putative isoform 2 [Theobroma cacao]
gi|508777421|gb|EOY24677.1| Nucleic acid-binding,
putative isoform 2 [Theobroma cacao]
Length = 588
Score = 171 bits (434), Expect = 3e-40
Identities = 116/294 (39%), Positives = 160/294 (54%), Gaps = 14/294 (4%)
Frame = +3
Query: 60 LRKPEAAVFNTTDDDN---------EHEKK---NSADFNSLRKPKAAVVSTTDDDNEHAK 203
LRKPE +T+ D E EK+ DF L K + ++ + + E +
Sbjct: 217 LRKPEPMSVDTSLDKKQDSEGSVGLEKEKEVEDRIGDFTILEKSEQSIHANIREMQELFE 276
Query: 204 KNSADLTRLSSGGFENSDDFNSLSSVDYNSILQ--KPNHAPFAKPLERSDVVLSVMDDVT 377
+ RL E + N L++ N+ + + H+ K ER D +S M V
Sbjct: 277 DLEIEGQRL-----EAEIEANMLANATENTSQESLEAGHSSIPKKPERKDDSISGMQPVE 331
Query: 378 AVKQPSDTNSSNSAIESTVEAALQGKPQRLNPSAKQSSVSLKEETNRVSDNIFSVSVDIG 557
+ S SS ++EAALQGKP+RL+ S K++S S + +T ++ D G
Sbjct: 332 LSNRVSTEESSTGP---SIEAALQGKPKRLDQSVKETSNSSRAQTVPINPE------DYG 382
Query: 558 DFIAAAPVKDQEDADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNLGSK 737
D + +P QED+DWTR +LLKT ER EVELIS STRGFV SFGSL+GFLPYRNL +K
Sbjct: 383 DLPSVSP---QEDSDWTRVEHLLKTGERAEVELISSSTRGFVVSFGSLIGFLPYRNLAAK 439
Query: 738 WKFLTFESWLRKKGLDLSMYKQNASIIGGYEAQTKSLPLDTNPSPATVGKFGGE 899
WKFL FESWLR+KGLDL+ YKQN +IG + +K+ L +N +F G+
Sbjct: 440 WKFLAFESWLRQKGLDLAAYKQNLGVIGSSDIMSKNSSLVSNSDMENNQQFEGK 493
>ref|XP_007040175.1| Nucleic acid-binding, OB-fold-like protein, putative isoform 1
[Theobroma cacao] gi|508777420|gb|EOY24676.1| Nucleic
acid-binding, OB-fold-like protein, putative isoform 1
[Theobroma cacao]
Length = 747
Score = 171 bits (434), Expect = 3e-40
Identities = 116/294 (39%), Positives = 160/294 (54%), Gaps = 14/294 (4%)
Frame = +3
Query: 60 LRKPEAAVFNTTDDDN---------EHEKK---NSADFNSLRKPKAAVVSTTDDDNEHAK 203
LRKPE +T+ D E EK+ DF L K + ++ + + E +
Sbjct: 217 LRKPEPMSVDTSLDKKQDSEGSVGLEKEKEVEDRIGDFTILEKSEQSIHANIREMQELFE 276
Query: 204 KNSADLTRLSSGGFENSDDFNSLSSVDYNSILQ--KPNHAPFAKPLERSDVVLSVMDDVT 377
+ RL E + N L++ N+ + + H+ K ER D +S M V
Sbjct: 277 DLEIEGQRL-----EAEIEANMLANATENTSQESLEAGHSSIPKKPERKDDSISGMQPVE 331
Query: 378 AVKQPSDTNSSNSAIESTVEAALQGKPQRLNPSAKQSSVSLKEETNRVSDNIFSVSVDIG 557
+ S SS ++EAALQGKP+RL+ S K++S S + +T ++ D G
Sbjct: 332 LSNRVSTEESSTGP---SIEAALQGKPKRLDQSVKETSNSSRAQTVPINPE------DYG 382
Query: 558 DFIAAAPVKDQEDADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNLGSK 737
D + +P QED+DWTR +LLKT ER EVELIS STRGFV SFGSL+GFLPYRNL +K
Sbjct: 383 DLPSVSP---QEDSDWTRVEHLLKTGERAEVELISSSTRGFVVSFGSLIGFLPYRNLAAK 439
Query: 738 WKFLTFESWLRKKGLDLSMYKQNASIIGGYEAQTKSLPLDTNPSPATVGKFGGE 899
WKFL FESWLR+KGLDL+ YKQN +IG + +K+ L +N +F G+
Sbjct: 440 WKFLAFESWLRQKGLDLAAYKQNLGVIGSSDIMSKNSSLVSNSDMENNQQFEGK 493
>ref|XP_007210897.1| hypothetical protein PRUPE_ppa001627mg [Prunus persica]
gi|462406632|gb|EMJ12096.1| hypothetical protein
PRUPE_ppa001627mg [Prunus persica]
Length = 717
Score = 168 bits (426), Expect = 2e-39
Identities = 116/317 (36%), Positives = 169/317 (53%), Gaps = 29/317 (9%)
Frame = +3
Query: 36 ENADDFNNLRKPEAAVFNTTDDDNEHEKKNSAD------------------------FNS 143
E D LRKP+A + D +E++K+ S+D F
Sbjct: 227 EMFSDMTLLRKPQAV---SVDKSSENKKEQSSDVDRNVIGDAELEKWREEENDEVSGFTL 283
Query: 144 LRKPKAAVVST-TDDDNEHAKKNSADLTR--LSSGGFENSDDFNSLSSVDYNSILQKPNH 314
L KP A V T +++DNE + + T + G ++ + S NS+ + +
Sbjct: 284 LEKPIAIGVETKSENDNEQLENQESSATDNVQDNNGLKDFYGSTATSEGTRNSLEESKDD 343
Query: 315 APFA-KPLERSDVVLSVMDDVTAVKQPSDTNSSNSAIESTVEAALQGKPQRLNPSAKQSS 491
+ + E+S + ++V+AV + SDTN S +E +++ ALQGKP+R + K++S
Sbjct: 344 SLIGLQQYEQS--TMESNEEVSAVSELSDTNLPVSNVELSIDTALQGKPKRFDIPVKEAS 401
Query: 492 VSLKEETNRVSDNI-FSVSVDIGDFIAAAPVKDQEDADWTRAGNLLKTEEREEVELISCS 668
V E S N+ F+ +++ F+ EDADW A NL+K +R +VELIS S
Sbjct: 402 VKEAESNLVESGNLLFASPIEVRLFVTFD--LGHEDADWVMAENLVKRGDRGDVELISAS 459
Query: 669 TRGFVASFGSLVGFLPYRNLGSKWKFLTFESWLRKKGLDLSMYKQNASIIGGYEAQTKSL 848
TRGFV SF SL+GFLPYRNL SKWKFL FESWLR+KGLD S+Y++N IIG Y+ K+
Sbjct: 460 TRGFVVSFRSLIGFLPYRNLASKWKFLAFESWLRRKGLDPSLYRRNLGIIGSYDIVDKNA 519
Query: 849 PLDTNPSPATVGKFGGE 899
L+ + P V K GE
Sbjct: 520 LLNPSLDPNVVIKNDGE 536
>ref|XP_007210896.1| hypothetical protein PRUPE_ppa001627mg [Prunus persica]
gi|462406631|gb|EMJ12095.1| hypothetical protein
PRUPE_ppa001627mg [Prunus persica]
Length = 790
Score = 168 bits (426), Expect = 2e-39
Identities = 116/317 (36%), Positives = 169/317 (53%), Gaps = 29/317 (9%)
Frame = +3
Query: 36 ENADDFNNLRKPEAAVFNTTDDDNEHEKKNSAD------------------------FNS 143
E D LRKP+A + D +E++K+ S+D F
Sbjct: 227 EMFSDMTLLRKPQAV---SVDKSSENKKEQSSDVDRNVIGDAELEKWREEENDEVSGFTL 283
Query: 144 LRKPKAAVVST-TDDDNEHAKKNSADLTR--LSSGGFENSDDFNSLSSVDYNSILQKPNH 314
L KP A V T +++DNE + + T + G ++ + S NS+ + +
Sbjct: 284 LEKPIAIGVETKSENDNEQLENQESSATDNVQDNNGLKDFYGSTATSEGTRNSLEESKDD 343
Query: 315 APFA-KPLERSDVVLSVMDDVTAVKQPSDTNSSNSAIESTVEAALQGKPQRLNPSAKQSS 491
+ + E+S + ++V+AV + SDTN S +E +++ ALQGKP+R + K++S
Sbjct: 344 SLIGLQQYEQS--TMESNEEVSAVSELSDTNLPVSNVELSIDTALQGKPKRFDIPVKEAS 401
Query: 492 VSLKEETNRVSDNI-FSVSVDIGDFIAAAPVKDQEDADWTRAGNLLKTEEREEVELISCS 668
V E S N+ F+ +++ F+ EDADW A NL+K +R +VELIS S
Sbjct: 402 VKEAESNLVESGNLLFASPIEVRLFVTFD--LGHEDADWVMAENLVKRGDRGDVELISAS 459
Query: 669 TRGFVASFGSLVGFLPYRNLGSKWKFLTFESWLRKKGLDLSMYKQNASIIGGYEAQTKSL 848
TRGFV SF SL+GFLPYRNL SKWKFL FESWLR+KGLD S+Y++N IIG Y+ K+
Sbjct: 460 TRGFVVSFRSLIGFLPYRNLASKWKFLAFESWLRRKGLDPSLYRRNLGIIGSYDIVDKNA 519
Query: 849 PLDTNPSPATVGKFGGE 899
L+ + P V K GE
Sbjct: 520 LLNPSLDPNVVIKNDGE 536
>ref|XP_006385758.1| hypothetical protein POPTR_0003s12430g [Populus trichocarpa]
gi|550343039|gb|ERP63555.1| hypothetical protein
POPTR_0003s12430g [Populus trichocarpa]
Length = 799
Score = 168 bits (425), Expect = 3e-39
Identities = 103/279 (36%), Positives = 154/279 (55%), Gaps = 23/279 (8%)
Frame = +3
Query: 105 NEHEKKNSADFNSLRKPKAAVVSTTDD-----------------DNEHAKKNSADLTRLS 233
N+ K+ +D LRKP+ V D + E + + T L
Sbjct: 213 NDQNKEKFSDMTLLRKPRPMSVDEKPDSGNLGTEVNHDGAGMRVEKEEGENRYSGFTLLK 272
Query: 234 SGG-----FENSDDFNSLSSVDYNSILQKPNHAPFAKPLERSDVVLSVMDDVTAVKQPSD 398
F+ S + S V+ + + N+ +P E+S++ + ++ A+ Q S
Sbjct: 273 KPKTMKIEFKESSETGDASFVEEQEV--EDNYISGRQPSEKSNIEFT--EEEAALNQQSG 328
Query: 399 TNSSNSAIESTVEAALQGKPQRLNPSAKQSSVSLKEETNRVS-DNIFSVSVDIGDFIAAA 575
N +SA++ ++EAALQGKP+RL+ + +S S E+ N V+ +N+ + + D+
Sbjct: 329 NNLVDSAVKISMEAALQGKPKRLDQYVEATSASRVEDLNLVNAENLGNANEDV------T 382
Query: 576 PVKDQEDADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNLGSKWKFLTF 755
+ EDADW RA +LL+T +R EVELIS S RGF+ SFGSLVGFLPYRNL ++WKFL F
Sbjct: 383 SISPLEDADWKRADDLLRTGDRVEVELISFSVRGFIVSFGSLVGFLPYRNLAARWKFLAF 442
Query: 756 ESWLRKKGLDLSMYKQNASIIGGYEAQTKSLPLDTNPSP 872
ESWL++KGLD S+YK+N IIG Y K+ LD++ P
Sbjct: 443 ESWLKQKGLDPSLYKKNLGIIGSYNVPEKNSSLDSSIVP 481
>ref|XP_002304482.2| S1 RNA-binding domain-containing family protein [Populus trichocarpa]
gi|550343038|gb|EEE79461.2| S1 RNA-binding
domain-containing family protein [Populus trichocarpa]
Length = 816
Score = 168 bits (425), Expect = 3e-39
Identities = 103/279 (36%), Positives = 154/279 (55%), Gaps = 23/279 (8%)
Frame = +3
Query: 105 NEHEKKNSADFNSLRKPKAAVVSTTDD-----------------DNEHAKKNSADLTRLS 233
N+ K+ +D LRKP+ V D + E + + T L
Sbjct: 230 NDQNKEKFSDMTLLRKPRPMSVDEKPDSGNLGTEVNHDGAGMRVEKEEGENRYSGFTLLK 289
Query: 234 SGG-----FENSDDFNSLSSVDYNSILQKPNHAPFAKPLERSDVVLSVMDDVTAVKQPSD 398
F+ S + S V+ + + N+ +P E+S++ + ++ A+ Q S
Sbjct: 290 KPKTMKIEFKESSETGDASFVEEQEV--EDNYISGRQPSEKSNIEFT--EEEAALNQQSG 345
Query: 399 TNSSNSAIESTVEAALQGKPQRLNPSAKQSSVSLKEETNRVS-DNIFSVSVDIGDFIAAA 575
N +SA++ ++EAALQGKP+RL+ + +S S E+ N V+ +N+ + + D+
Sbjct: 346 NNLVDSAVKISMEAALQGKPKRLDQYVEATSASRVEDLNLVNAENLGNANEDV------T 399
Query: 576 PVKDQEDADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNLGSKWKFLTF 755
+ EDADW RA +LL+T +R EVELIS S RGF+ SFGSLVGFLPYRNL ++WKFL F
Sbjct: 400 SISPLEDADWKRADDLLRTGDRVEVELISFSVRGFIVSFGSLVGFLPYRNLAARWKFLAF 459
Query: 756 ESWLRKKGLDLSMYKQNASIIGGYEAQTKSLPLDTNPSP 872
ESWL++KGLD S+YK+N IIG Y K+ LD++ P
Sbjct: 460 ESWLKQKGLDPSLYKKNLGIIGSYNVPEKNSSLDSSIVP 498
>ref|XP_006352282.1| PREDICTED: uncharacterized protein LOC102596782 [Solanum tuberosum]
Length = 912
Score = 167 bits (424), Expect = 4e-39
Identities = 102/256 (39%), Positives = 151/256 (58%), Gaps = 3/256 (1%)
Frame = +3
Query: 114 EKKNSADFNSLRKPKAAVVSTTDDDNEHAKKNSADLTRLSSGGFENSDDFNS-LSSVDYN 290
E KNS N KP+ + ++ D N+ + + A +S N+ + +S L S+ N
Sbjct: 400 EPKNSLFLN---KPEPSNLNLKIDPNQESSE--AQHPSISDESTLNAANSSSELISMAEN 454
Query: 291 SILQKPNHAPFAKPLERSDVVLSVMD-DVTAVKQPSDTNS-SNSAIESTVEAALQGKPQR 464
L++P + + PLE+ D ++ SD+N+ S + +++AAL+GKP+R
Sbjct: 455 K-LRQPLQSSRSNPLEKQGFGTGFQQTDTQPAERSSDSNTPSKTGPMESLDAALRGKPKR 513
Query: 465 LNPSAKQSSVSLKEETNRVSDNIFSVSVDIGDFIAAAPVKDQEDADWTRAGNLLKTEERE 644
L+ K++S KE+ V + + +I +F+A + K+ ED DW RA L+K+ RE
Sbjct: 514 LDQPKKEASSVSKEDMRPVKSEGYGNASEIENFLAKSSTKEHEDNDWVRAEELVKSGGRE 573
Query: 645 EVELISCSTRGFVASFGSLVGFLPYRNLGSKWKFLTFESWLRKKGLDLSMYKQNASIIGG 824
+VEL+SCSTRGFV SFGSL+GFLPYRNL ++WKFL FESWLR+KGLD S YKQ IIGG
Sbjct: 574 DVELVSCSTRGFVVSFGSLIGFLPYRNLAARWKFLAFESWLRQKGLDPSQYKQGLGIIGG 633
Query: 825 YEAQTKSLPLDTNPSP 872
Y+ K+ + P
Sbjct: 634 YDGFGKAASPEAGVDP 649
>ref|XP_004244605.1| PREDICTED: uncharacterized protein LOC101250822 [Solanum
lycopersicum]
Length = 883
Score = 167 bits (422), Expect = 7e-39
Identities = 114/331 (34%), Positives = 165/331 (49%), Gaps = 52/331 (15%)
Frame = +3
Query: 36 ENADDFNNLRKPEAAVFNTTDDD-NEHEKKNSADFNSLRKPKAAVVSTTD---------- 182
E D L+KPE ++ D + N S+D L+KP+ +S+ D
Sbjct: 290 EKFSDVTLLKKPEPMRISSDDSEKNGQSSDKSSDATLLKKPEPMRISSDDSEKNGQSSDV 349
Query: 183 -----DDNEHAK-----------KNSADLTRLSSGGFENSDDFNSLSS-VDYNSI----- 296
DD+E A KNS L + D N SS V + SI
Sbjct: 350 LPVSSDDSEDASLTEVYASSSEPKNSLLLNKPEPSNLNLKIDPNKESSEVQHPSISDEST 409
Query: 297 -----------------LQKPNHAPFAKPLERSDVVLSVMD-DVTAVKQPSDTNS-SNSA 419
L++P + + PLE+ D ++ SD+N+ + +
Sbjct: 410 FDAANSSSELISMAESKLRQPLQSNRSNPLEKQGFGTGFQQTDTQPAERSSDSNTPAETG 469
Query: 420 IESTVEAALQGKPQRLNPSAKQSSVSLKEETNRVSDNIFSVSVDIGDFIAAAPVKDQEDA 599
+++AAL GKP+RL+ K++S +E+ V + + +I +F+A + +K+ ED
Sbjct: 470 PMESLDAALLGKPKRLDRPKKEASRVSQEDMRPVKSEGYGNASEIENFLAKSSIKEHEDN 529
Query: 600 DWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNLGSKWKFLTFESWLRKKG 779
DW RA L+K+ RE+VEL+SCSTRGFV SFGSL+GFLPYRNL ++WKFL FESWLR+KG
Sbjct: 530 DWVRAEELVKSGGREDVELVSCSTRGFVVSFGSLIGFLPYRNLAARWKFLAFESWLRQKG 589
Query: 780 LDLSMYKQNASIIGGYEAQTKSLPLDTNPSP 872
LD S YKQ IIGGY+ K+ + P
Sbjct: 590 LDPSQYKQGLGIIGGYDGFGKAASPEAGVDP 620
>ref|XP_007160042.1| hypothetical protein PHAVU_002G287600g [Phaseolus vulgaris]
gi|561033457|gb|ESW32036.1| hypothetical protein
PHAVU_002G287600g [Phaseolus vulgaris]
Length = 732
Score = 164 bits (415), Expect = 4e-38
Identities = 98/278 (35%), Positives = 151/278 (54%), Gaps = 17/278 (6%)
Frame = +3
Query: 105 NEHEKKNSADFNSLRKPKAAVVSTTD-----DDNEHAKKNSADLTRLSSGGFENSDDFNS 269
+E K+ ++ L+KP+ V +TD N N +L + + F
Sbjct: 205 DEQVKEKFSNMTLLKKPEPPVAKSTDMVEETSSNVDQGNNDGELKMWNEERGDEISGFTL 264
Query: 270 LSSVDYNSILQKPNH-------APFAKPLERSDVVLSVMDDVTAVKQPSDTNSSNSAIES 428
L S+ ++ P A+P + + L + + + D S++S +E
Sbjct: 265 LERPHKPSVEKEEEEFEEVNAMVPNAEPEQHEQLELHQAPN--DLSESLDIKSADSGLEL 322
Query: 429 TVEAALQGKPQRLNPSAKQSSVSLKEETN-----RVSDNIFSVSVDIGDFIAAAPVKDQE 593
V+AALQ KP+RL+ K++S ++E T+ + S++ + D+ DF ++ E
Sbjct: 323 PVDAALQAKPKRLDQYVKRTSKFVEEGTSLNLGGQTSNDNLGNAADVSDF------QESE 376
Query: 594 DADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNLGSKWKFLTFESWLRK 773
DADWTRA L++T +RE+VEL+SC+T+GF+ SFGSLVGFLPYRNL SKWKF FE+WL++
Sbjct: 377 DADWTRAEGLIETGDREDVELVSCNTKGFIVSFGSLVGFLPYRNLNSKWKFFAFETWLKQ 436
Query: 774 KGLDLSMYKQNASIIGGYEAQTKSLPLDTNPSPATVGK 887
KGLD SM+KQN+ ++ K D+ PSP GK
Sbjct: 437 KGLDPSMFKQNSGTFTSFDTDIKIFSPDSPPSPEIDGK 474
>ref|XP_006417193.1| hypothetical protein EUTSA_v10006889mg [Eutrema salsugineum]
gi|557094964|gb|ESQ35546.1| hypothetical protein
EUTSA_v10006889mg [Eutrema salsugineum]
Length = 746
Score = 164 bits (415), Expect = 4e-38
Identities = 108/316 (34%), Positives = 160/316 (50%), Gaps = 41/316 (12%)
Frame = +3
Query: 60 LRKPEAAVFNTTDDD---------------NEHEKKNSADFNSLRKPKAAVVSTTDDDNE 194
LRKP + N DD+ NE E + +D LRKP+ V ++
Sbjct: 185 LRKPSSYYVNNNDDEESKLRLKPNLTLKMRNERENERFSDMTLLRKPEPLNVDAGEE--- 241
Query: 195 HAKKNSADLTRLSSGGFENSDDFNSLSSVDYNSILQKPNHAPFAKPLER----------- 341
NS D +L S G + ++L+KP P + +E
Sbjct: 242 ----NSGD--KLPSDGLTMEEGEQDEGVYSEYTLLEKPEPRPEPENVEEEAGDSGAVELP 295
Query: 342 --------------SDVVLSVMDDVTAVKQPSDTNSSNSAIESTVEAALQGKPQRLNPSA 479
S++ ++ T P + + SN ++ VEA+L GKPQRL+PS+
Sbjct: 296 EIENTSIPTEMQLNSEISSGSSEEKTINSDPVERSPSNPVSQTIVEASLLGKPQRLDPSS 355
Query: 480 KQSSVSLKEETNRVSDNIFSVSVDIGDFIAAAPVKDQ-EDADWTRAGNLLKTEEREEVEL 656
+ SVS + + V+ VSV++ P + E++DW +A +L+KTE R +VEL
Sbjct: 356 AEPSVSTRGQPLIVNHEGRQVSVEL----KGPPSRSSLEESDWNKAESLVKTELRADVEL 411
Query: 657 ISCSTRGFVASFGSLVGFLPYRNLGSKWKFLTFESWLRKKGLDLSMYKQNASIIGGYEAQ 836
IS STRGF S+GSL+GFLPYRNL +KWKFL FESWLR+KG+D S+Y+QN +IGG +
Sbjct: 412 ISSSTRGFAVSYGSLIGFLPYRNLAAKWKFLAFESWLRRKGVDPSLYRQNLGVIGGQDVT 471
Query: 837 TKSLPLDTNPSPATVG 884
+K+ D++ + VG
Sbjct: 472 SKAPTPDSSLVDSEVG 487
>ref|XP_002509767.1| conserved hypothetical protein [Ricinus communis]
gi|223549666|gb|EEF51154.1| conserved hypothetical
protein [Ricinus communis]
Length = 748
Score = 164 bits (414), Expect = 6e-38
Identities = 111/297 (37%), Positives = 155/297 (52%), Gaps = 9/297 (3%)
Frame = +3
Query: 36 ENADDFNNLRKPEAAVFNTTDDDNEHEKKNSADFNSLRKPKAAVVSTTDDDNEHAKKNSA 215
E D LRKPE N EK+ S D + A T + + K +
Sbjct: 219 EKFSDMTLLRKPEPV--------NVEEKQESLDGAETKISNGATELGTGKEEDDIKYSGF 270
Query: 216 DLTRLSSGGFENSDDFNSL--SSVDYNSILQ---KPNHAPFA----KPLERSDVVLSVMD 368
L + + D+ + SSV L+ K N F +PLE+S++ D
Sbjct: 271 TLLKKPETSVSDVDESSETVGSSVPKEQELEVGIKKNSFLFCFEGMQPLEKSNI--GPTD 328
Query: 369 DVTAVKQPSDTNSSNSAIESTVEAALQGKPQRLNPSAKQSSVSLKEETNRVSDNIFSVSV 548
D SD + +++ +V+ LQGKP+RL+ K++ S +EET + + +
Sbjct: 329 D------QSDKKLVDDSVKFSVDTTLQGKPKRLDQYVKETLASTREETTLLHPESYGNAD 382
Query: 549 DIGDFIAAAPVKDQEDADWTRAGNLLKTEEREEVELISCSTRGFVASFGSLVGFLPYRNL 728
++ + +P+ EDADW+RA +L KT R EVEL+S STRGF+ SFGSLVGFLPYRNL
Sbjct: 383 ELKNLPPISPI---EDADWSRAEDLFKTGNRGEVELVSASTRGFIVSFGSLVGFLPYRNL 439
Query: 729 GSKWKFLTFESWLRKKGLDLSMYKQNASIIGGYEAQTKSLPLDTNPSPATVGKFGGE 899
+KWKFL FESWL++KGLD SMYKQN IIG Y+ K+ D++ K GGE
Sbjct: 440 VAKWKFLAFESWLKQKGLDPSMYKQNLGIIGSYDVLDKN--FDSSADQEINKKIGGE 494
>ref|XP_006417194.1| hypothetical protein EUTSA_v10006889mg [Eutrema salsugineum]
gi|557094965|gb|ESQ35547.1| hypothetical protein
EUTSA_v10006889mg [Eutrema salsugineum]
Length = 755
Score = 160 bits (406), Expect = 5e-37
Identities = 108/325 (33%), Positives = 160/325 (49%), Gaps = 50/325 (15%)
Frame = +3
Query: 60 LRKPEAAVFNTTDDD---------------NEHEKKNSADFNSLRKPKAAVVSTTDDDNE 194
LRKP + N DD+ NE E + +D LRKP+ V ++
Sbjct: 185 LRKPSSYYVNNNDDEESKLRLKPNLTLKMRNERENERFSDMTLLRKPEPLNVDAGEE--- 241
Query: 195 HAKKNSADLTRLSSGGFENSDDFNSLSSVDYNSILQKPNHAPFAKPLER----------- 341
NS D +L S G + ++L+KP P + +E
Sbjct: 242 ----NSGD--KLPSDGLTMEEGEQDEGVYSEYTLLEKPEPRPEPENVEEEAGDSGAVELP 295
Query: 342 -----------------------SDVVLSVMDDVTAVKQPSDTNSSNSAIESTVEAALQG 452
S++ ++ T P + + SN ++ VEA+L G
Sbjct: 296 EIENTSIPTGMGNTSIPTEMQLNSEISSGSSEEKTINSDPVERSPSNPVSQTIVEASLLG 355
Query: 453 KPQRLNPSAKQSSVSLKEETNRVSDNIFSVSVDIGDFIAAAPVKDQ-EDADWTRAGNLLK 629
KPQRL+PS+ + SVS + + V+ VSV++ P + E++DW +A +L+K
Sbjct: 356 KPQRLDPSSAEPSVSTRGQPLIVNHEGRQVSVEL----KGPPSRSSLEESDWNKAESLVK 411
Query: 630 TEEREEVELISCSTRGFVASFGSLVGFLPYRNLGSKWKFLTFESWLRKKGLDLSMYKQNA 809
TE R +VELIS STRGF S+GSL+GFLPYRNL +KWKFL FESWLR+KG+D S+Y+QN
Sbjct: 412 TELRADVELISSSTRGFAVSYGSLIGFLPYRNLAAKWKFLAFESWLRRKGVDPSLYRQNL 471
Query: 810 SIIGGYEAQTKSLPLDTNPSPATVG 884
+IGG + +K+ D++ + VG
Sbjct: 472 GVIGGQDVTSKAPTPDSSLVDSEVG 496