BLASTX nr result
ID: Papaver27_contig00004420
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver27_contig00004420
(564 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002280972.1| PREDICTED: uncharacterized protein LOC100249... 186 4e-45
ref|XP_006475612.1| PREDICTED: uncharacterized protein LOC102613... 185 8e-45
ref|XP_002324991.1| hypothetical protein POPTR_0018s08760g [Popu... 181 9e-44
ref|XP_003528926.1| PREDICTED: uncharacterized protein LOC100790... 181 2e-43
ref|XP_007013173.1| Uncharacterized protein TCM_037877 [Theobrom... 179 4e-43
gb|EXB79939.1| hypothetical protein L484_002193 [Morus notabilis] 178 1e-42
ref|XP_004251605.1| PREDICTED: uncharacterized protein LOC101249... 177 1e-42
ref|XP_006353458.1| PREDICTED: uncharacterized protein LOC102593... 177 2e-42
ref|XP_004512979.1| PREDICTED: uncharacterized protein LOC101490... 176 4e-42
ref|NP_001242080.1| uncharacterized protein LOC100795956 [Glycin... 176 5e-42
ref|XP_004170585.1| PREDICTED: uncharacterized protein LOC101225... 174 2e-41
ref|XP_004150055.1| PREDICTED: uncharacterized protein LOC101202... 174 2e-41
ref|XP_007152833.1| hypothetical protein PHAVU_004G163700g [Phas... 172 7e-41
gb|EYU23019.1| hypothetical protein MIMGU_mgv1a006454mg [Mimulus... 171 9e-41
ref|XP_004287418.1| PREDICTED: uncharacterized protein LOC101301... 170 3e-40
gb|AFK48173.1| unknown [Medicago truncatula] 169 3e-40
gb|AFK36517.1| unknown [Medicago truncatula] 169 3e-40
ref|XP_003620670.1| hypothetical protein MTR_6g088460 [Medicago ... 169 3e-40
dbj|BAD05438.1| unknown protein [Oryza sativa Japonica Group] gi... 169 5e-40
ref|NP_001061714.2| Os08g0387500 [Oryza sativa Japonica Group] g... 169 5e-40
>ref|XP_002280972.1| PREDICTED: uncharacterized protein LOC100249177 [Vitis vinifera]
gi|297738826|emb|CBI28071.3| unnamed protein product
[Vitis vinifera]
Length = 444
Score = 186 bits (472), Expect = 4e-45
Identities = 82/110 (74%), Positives = 100/110 (90%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD++PSQV+WVTAWSIRN+ L ++PFLK +AEV+GWPL
Sbjct: 335 IAILYGGGHMPDLGRRLREEFDLIPSQVQWVTAWSIRNRYLKSSSLPFLKAMAEVSGWPL 394
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVEDSKLL 332
NRYQTLAL IFSSVLALDLWFWELFFGT V+W+SH S++ Q+VE+S+L+
Sbjct: 395 NRYQTLALAIFSSVLALDLWFWELFFGTAVNWVSHLGSQVVQYVENSRLM 444
>ref|XP_006475612.1| PREDICTED: uncharacterized protein LOC102613449 [Citrus sinensis]
Length = 461
Score = 185 bits (469), Expect = 8e-45
Identities = 82/105 (78%), Positives = 96/105 (91%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD++PS+V+W+TAWSIRN++L + PFLKT+AEV GWPL
Sbjct: 355 IAILYGGGHMPDLGRRLREEFDLLPSRVQWITAWSIRNRDLSSSSFPFLKTMAEVLGWPL 414
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVE 317
NRYQTLALLIFSSVLALDLWFWELFFGTTV+WIS SE+ Q+VE
Sbjct: 415 NRYQTLALLIFSSVLALDLWFWELFFGTTVNWISQIASEVHQYVE 459
>ref|XP_002324991.1| hypothetical protein POPTR_0018s08760g [Populus trichocarpa]
gi|222866425|gb|EEF03556.1| hypothetical protein
POPTR_0018s08760g [Populus trichocarpa]
Length = 468
Score = 181 bits (460), Expect = 9e-44
Identities = 79/110 (71%), Positives = 98/110 (89%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD+VP V+W+TAWSIRN+ L ++PFL+T+AEV GWPL
Sbjct: 358 IAILYGGGHMPDLGRRLREEFDLVPCGVQWITAWSIRNRVLNSNSLPFLRTMAEVLGWPL 417
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVEDSKLL 332
NRYQTLALLIFSSVLA+DLWFWELFFG+TV+WIS SE+ Q+V +S+++
Sbjct: 418 NRYQTLALLIFSSVLAIDLWFWELFFGSTVNWISQIASEVGQYVNNSRMI 467
>ref|XP_003528926.1| PREDICTED: uncharacterized protein LOC100790976 [Glycine max]
Length = 464
Score = 181 bits (458), Expect = 2e-43
Identities = 79/110 (71%), Positives = 98/110 (89%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLREDFD+VPS V+W+TAWSI K+L ++ PFLKT+A+ +GWPL
Sbjct: 355 IAILYGGGHMPDLGRRLREDFDLVPSNVQWITAWSITKKDLNTDSFPFLKTIAKASGWPL 414
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVEDSKLL 332
NRYQTLALLIFSSVLALDLWFWELFFGT V+W+S SE+ Q+V++S+++
Sbjct: 415 NRYQTLALLIFSSVLALDLWFWELFFGTAVNWVSELGSELLQYVDNSQMI 464
>ref|XP_007013173.1| Uncharacterized protein TCM_037877 [Theobroma cacao]
gi|508783536|gb|EOY30792.1| Uncharacterized protein
TCM_037877 [Theobroma cacao]
Length = 487
Score = 179 bits (454), Expect = 4e-43
Identities = 78/109 (71%), Positives = 99/109 (90%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD+VPSQV+W+TAWSI ++L ++PFLKT+AEV+GWPL
Sbjct: 352 IAILYGGGHMPDLGRRLREEFDLVPSQVQWITAWSITKRDLESSSLPFLKTMAEVSGWPL 411
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVEDSKL 329
NRYQTLALLIFSSVLALDLWFWELF T V+WIS VSE++Q+++++++
Sbjct: 412 NRYQTLALLIFSSVLALDLWFWELFCSTAVNWISEVVSEVAQYIDNAQV 460
>gb|EXB79939.1| hypothetical protein L484_002193 [Morus notabilis]
Length = 453
Score = 178 bits (451), Expect = 1e-42
Identities = 77/109 (70%), Positives = 97/109 (88%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD+VPS+V W+TAWSIRNK+L ++PFLKT+A+++GWPL
Sbjct: 345 IAILYGGGHMPDLGRRLREEFDLVPSRVRWITAWSIRNKDLKSSSLPFLKTMAKISGWPL 404
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVEDSKL 329
NRYQTLALLIFSSVLALDLWFWE+F GT VDWIS S+ F++++++
Sbjct: 405 NRYQTLALLIFSSVLALDLWFWEIFCGTAVDWISQFASDFVHFIDNAQI 453
>ref|XP_004251605.1| PREDICTED: uncharacterized protein LOC101249241 [Solanum
lycopersicum]
Length = 449
Score = 177 bits (450), Expect = 1e-42
Identities = 79/104 (75%), Positives = 93/104 (89%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD+VPS+V+W+TAWSI N+ L ++P LK +AEV+GWPL
Sbjct: 344 IAILYGGGHMPDLGRRLREEFDLVPSEVQWITAWSITNRNLTTNSLPLLKQMAEVSGWPL 403
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFV 314
NRYQTLALLIFSSVLALDLWFWELFFGTT +WIS S+ISQ+V
Sbjct: 404 NRYQTLALLIFSSVLALDLWFWELFFGTTANWISQVASDISQYV 447
>ref|XP_006353458.1| PREDICTED: uncharacterized protein LOC102593492 [Solanum tuberosum]
Length = 451
Score = 177 bits (449), Expect = 2e-42
Identities = 78/104 (75%), Positives = 93/104 (89%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD+VPS+V+W+TAWSI N+ L ++P LK +AEV+GWPL
Sbjct: 344 IAILYGGGHMPDLGRRLREEFDLVPSEVQWITAWSITNRNLTTNSLPLLKQMAEVSGWPL 403
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFV 314
NRYQTLALLIFSSVLALDLWFWELFFGTT +W+S S+ISQ+V
Sbjct: 404 NRYQTLALLIFSSVLALDLWFWELFFGTTANWVSQVASDISQYV 447
>ref|XP_004512979.1| PREDICTED: uncharacterized protein LOC101490777 [Cicer arietinum]
Length = 459
Score = 176 bits (446), Expect = 4e-42
Identities = 78/110 (70%), Positives = 95/110 (86%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD+VPS V+W+ AWSIR K+L ++PFLKT+A+ +GWPL
Sbjct: 350 IAILYGGGHMPDLGRRLREEFDLVPSGVQWLNAWSIRKKKLNTNSLPFLKTMAKASGWPL 409
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVEDSKLL 332
NRYQTLALLIFSSVLALDLWFWELFFGT V+W S SE+ Q+V +S ++
Sbjct: 410 NRYQTLALLIFSSVLALDLWFWELFFGTAVNWFSEAASEVLQYVGNSPMI 459
>ref|NP_001242080.1| uncharacterized protein LOC100795956 [Glycine max]
gi|255641605|gb|ACU21075.1| unknown [Glycine max]
Length = 464
Score = 176 bits (445), Expect = 5e-42
Identities = 77/110 (70%), Positives = 96/110 (87%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLREDFD+VPS V+W+TAWSI K+L ++ PFLKT+A+ +GWPL
Sbjct: 355 IAILYGGGHMPDLGRRLREDFDLVPSNVQWITAWSITKKDLNTDSFPFLKTIAKASGWPL 414
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVEDSKLL 332
NRYQTLAL IFSSVLALDLWFWELFFGT V+W+S SE+ ++V++S ++
Sbjct: 415 NRYQTLALPIFSSVLALDLWFWELFFGTAVNWVSELGSELLRYVDNSPMI 464
>ref|XP_004170585.1| PREDICTED: uncharacterized protein LOC101225879, partial [Cucumis
sativus]
Length = 437
Score = 174 bits (440), Expect = 2e-41
Identities = 74/110 (67%), Positives = 96/110 (87%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD++P +V+W+TAWSI ++LG ++PFLK LA+V+GWPL
Sbjct: 328 IAILYGGGHMPDLGRRLREEFDLIPCRVKWITAWSITKRKLGSSSLPFLKALADVSGWPL 387
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVEDSKLL 332
NRYQTLALLIFSSVLA+DLWFWELFFGT +WIS E+ Q++++ +L+
Sbjct: 388 NRYQTLALLIFSSVLAVDLWFWELFFGTAANWISEVALEVYQYIDNVQLM 437
>ref|XP_004150055.1| PREDICTED: uncharacterized protein LOC101202949, partial [Cucumis
sativus]
Length = 444
Score = 174 bits (440), Expect = 2e-41
Identities = 74/110 (67%), Positives = 96/110 (87%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD++P +V+W+TAWSI ++LG ++PFLK LA+V+GWPL
Sbjct: 335 IAILYGGGHMPDLGRRLREEFDLIPCRVKWITAWSITKRKLGSSSLPFLKALADVSGWPL 394
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVEDSKLL 332
NRYQTLALLIFSSVLA+DLWFWELFFGT +WIS E+ Q++++ +L+
Sbjct: 395 NRYQTLALLIFSSVLAVDLWFWELFFGTAANWISEVALEVYQYIDNVQLM 444
>ref|XP_007152833.1| hypothetical protein PHAVU_004G163700g [Phaseolus vulgaris]
gi|561026142|gb|ESW24827.1| hypothetical protein
PHAVU_004G163700g [Phaseolus vulgaris]
Length = 466
Score = 172 bits (435), Expect = 7e-41
Identities = 75/110 (68%), Positives = 95/110 (86%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD+VPS V+W+TAWSIR ++L + PFLK +A+ +GWPL
Sbjct: 357 IAILYGGGHMPDLGRRLREEFDLVPSNVKWITAWSIRKRDLNTNSFPFLKKIAKASGWPL 416
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVEDSKLL 332
NRYQTLALLIFSSVLA+DLWFWELFFGT ++W+S SE+ Q+V +S ++
Sbjct: 417 NRYQTLALLIFSSVLAVDLWFWELFFGTALNWVSEFGSELVQYVGNSPMI 466
>gb|EYU23019.1| hypothetical protein MIMGU_mgv1a006454mg [Mimulus guttatus]
Length = 443
Score = 171 bits (434), Expect = 9e-41
Identities = 77/105 (73%), Positives = 91/105 (86%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLREDFD+VPSQV+W+TAWSIR+K L + P LK LA+V GWPL
Sbjct: 333 IAILYGGGHMPDLGRRLREDFDLVPSQVQWITAWSIRDKNLSSRSFPVLKRLAQVFGWPL 392
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVE 317
NRYQTLALLIFSSVLA+DLWFWELFF T+V W+S+ S++ QF +
Sbjct: 393 NRYQTLALLIFSSVLAVDLWFWELFFDTSVSWVSNVGSDLLQFFD 437
>ref|XP_004287418.1| PREDICTED: uncharacterized protein LOC101301808 [Fragaria vesca
subsp. vesca]
Length = 460
Score = 170 bits (430), Expect = 3e-40
Identities = 75/110 (68%), Positives = 97/110 (88%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRL+E D++PS+ +WVTAWSI+N+ L ++PFLKT+AEV+GWPL
Sbjct: 351 IAILYGGGHMPDLGRRLQE-LDLIPSRAQWVTAWSIKNRNLNSNSLPFLKTMAEVSGWPL 409
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFVEDSKLL 332
NRYQTLALLIFSSVLA+DLWFWELFFGT VD+ S S++SQF++++ ++
Sbjct: 410 NRYQTLALLIFSSVLAIDLWFWELFFGTAVDFFSQIGSQVSQFIDNADMI 459
>gb|AFK48173.1| unknown [Medicago truncatula]
Length = 451
Score = 169 bits (429), Expect = 3e-40
Identities = 75/104 (72%), Positives = 90/104 (86%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD+VP VEWVTAWSIR ++L ++PFLKT+A +GWPL
Sbjct: 346 IAILYGGGHMPDLGRRLREEFDLVPYGVEWVTAWSIRKRKLDTSSLPFLKTMARASGWPL 405
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFV 314
NRYQTLALLIFSS+LA+DLWFWELFFGT V W+S +EI ++V
Sbjct: 406 NRYQTLALLIFSSILAVDLWFWELFFGTMVTWVSDVTTEILRYV 449
>gb|AFK36517.1| unknown [Medicago truncatula]
Length = 154
Score = 169 bits (429), Expect = 3e-40
Identities = 75/104 (72%), Positives = 90/104 (86%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD+VP VEWVTAWSIR ++L ++PFLKT+A +GWPL
Sbjct: 49 IAILYGGGHMPDLGRRLREEFDLVPYGVEWVTAWSIRKRKLDTSSLPFLKTMARASGWPL 108
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFV 314
NRYQTLALLIFSS+LA+DLWFWELFFGT V W+S +EI ++V
Sbjct: 109 NRYQTLALLIFSSILAVDLWFWELFFGTMVTWVSDVTTEILRYV 152
>ref|XP_003620670.1| hypothetical protein MTR_6g088460 [Medicago truncatula]
gi|355495685|gb|AES76888.1| hypothetical protein
MTR_6g088460 [Medicago truncatula]
Length = 488
Score = 169 bits (429), Expect = 3e-40
Identities = 75/104 (72%), Positives = 90/104 (86%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IA+LYGGGHMPDLGRRLRE+FD+VP VEWVTAWSIR ++L ++PFLKT+A +GWPL
Sbjct: 346 IAILYGGGHMPDLGRRLREEFDLVPYGVEWVTAWSIRKRKLDTSSLPFLKTMARASGWPL 405
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQFV 314
NRYQTLALLIFSS+LA+DLWFWELFFGT V W+S +EI ++V
Sbjct: 406 NRYQTLALLIFSSILAVDLWFWELFFGTMVTWVSDVTTEILRYV 449
>dbj|BAD05438.1| unknown protein [Oryza sativa Japonica Group]
gi|125603277|gb|EAZ42602.1| hypothetical protein
OsJ_27166 [Oryza sativa Japonica Group]
gi|215740441|dbj|BAG97097.1| unnamed protein product
[Oryza sativa Japonica Group]
Length = 440
Score = 169 bits (428), Expect = 5e-40
Identities = 78/103 (75%), Positives = 91/103 (88%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IAVLYGGGHMPDLGRRLRE+ +MVP+ V+WVTAWSIR++EL RE++PFLKT+AEV+GWPL
Sbjct: 334 IAVLYGGGHMPDLGRRLREELNMVPADVQWVTAWSIRSRELDRESLPFLKTMAEVSGWPL 393
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQF 311
NRY+TLALLIFSSVLA+DLWFWEL GT V+W S S I QF
Sbjct: 394 NRYETLALLIFSSVLAVDLWFWELLVGTAVNWASLAGSWIEQF 436
>ref|NP_001061714.2| Os08g0387500 [Oryza sativa Japonica Group]
gi|255678415|dbj|BAF23628.2| Os08g0387500, partial
[Oryza sativa Japonica Group]
Length = 486
Score = 169 bits (428), Expect = 5e-40
Identities = 78/103 (75%), Positives = 91/103 (88%)
Frame = +3
Query: 3 IAVLYGGGHMPDLGRRLREDFDMVPSQVEWVTAWSIRNKELGREAIPFLKTLAEVTGWPL 182
IAVLYGGGHMPDLGRRLRE+ +MVP+ V+WVTAWSIR++EL RE++PFLKT+AEV+GWPL
Sbjct: 380 IAVLYGGGHMPDLGRRLREELNMVPADVQWVTAWSIRSRELDRESLPFLKTMAEVSGWPL 439
Query: 183 NRYQTLALLIFSSVLALDLWFWELFFGTTVDWISHTVSEISQF 311
NRY+TLALLIFSSVLA+DLWFWEL GT V+W S S I QF
Sbjct: 440 NRYETLALLIFSSVLAVDLWFWELLVGTAVNWASLAGSWIEQF 482