BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver25_contig00029787
         (1369 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName...   720   0.0  
gb|AGL44333.1| FAD-dependent oxidoreductase, partial [Papaver so...   718   0.0  
gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]          612   e-172
gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]          592   e-166
sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName...   572   e-160
pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a ...   572   e-160
pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Comp...   572   e-160
pdb|3D2D|A Chain A, Structure Of Berberine Bridge Enzyme In Comp...   572   e-160
pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Comp...   570   e-160
pdb|3FW7|A Chain A, Structure Of Berberine Bridge Enzyme, H104a ...   570   e-160
pdb|3FWA|A Chain A, Structure Of Berberine Bridge Enzyme, C166a ...   568   e-159
pdb|3FW8|A Chain A, Structure Of Berberine Bridge Enzyme, C166a ...   566   e-159
gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera]         491   e-136
gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp....   471   e-130
dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica]             461   e-127
ref|XP_003634619.1| PREDICTED: LOW QUALITY PROTEIN: reticuline o...   360   1e-96
ref|XP_003555533.1| PREDICTED: reticuline oxidase-like [Glycine ...   357   5e-96
ref|XP_007143918.1| hypothetical protein PHAVU_007G113300g [Phas...   357   7e-96
ref|XP_003535410.1| PREDICTED: reticuline oxidase-like [Glycine ...   353   8e-95
ref|XP_002533924.1| d-lactate dehydrogenase, putative [Ricinus c...   353   8e-95

>sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName: Full=Berberine
            bridge-forming enzyme; Short=BBE; AltName:
            Full=Tetrahydroprotoberberine synthase; Flags: Precursor
            gi|3282517|gb|AAC61839.1| berberine bridge enzyme
            [Papaver somniferum]
          Length = 535

 Score =  720 bits (1859), Expect = 0.0
 Identities = 358/414 (86%), Positives = 375/414 (90%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            VIVDMMN+NR+SIDV SETA VESGATLGELYYAIAQST+TLGFTAGWCPTV        
Sbjct: 122  VIVDMMNLNRISIDVLSETAWVESGATLGELYYAIAQSTDTLGFTAGWCPTVGSGGHISG 181

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILIDS GAILDREKMG+DVFWAIR             KIK
Sbjct: 182  GGFGMMSRKYGLAADNVVDAILIDSNGAILDREKMGDDVFWAIRGGGGGVWGAIYAWKIK 241

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEKLTVFRVTKNVGIE ASSLLHKWQYVADELD DFTVSVLGGVNGN+AWLMFLGL
Sbjct: 242  LLPVPEKLTVFRVTKNVGIEDASSLLHKWQYVADELDEDFTVSVLGGVNGNDAWLMFLGL 301

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            HLGRKDAAK+II+EKFPELGLV+KEFQEMSWG SMAFL+GL+TISELNNRFLKFD+RAFK
Sbjct: 302  HLGRKDAAKTIIDEKFPELGLVDKEFQEMSWGESMAFLSGLDTISELNNRFLKFDERAFK 361

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVDFTK +VPL+VFR ALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE
Sbjct: 362  TKVDFTKVSVPLNVFRHALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 421

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YIIAWNQDEE+KIGEFSEWL KFYDYLEPFVSKEPRVGYVNHIDLDIGGIDW+NKSST+N
Sbjct: 422  YIIAWNQDEESKIGEFSEWLAKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWRNKSSTTN 481

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEENYILNEL 1243
            AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEE Y+L EL
Sbjct: 482  AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEEIYMLKEL 535


>gb|AGL44333.1| FAD-dependent oxidoreductase, partial [Papaver somniferum]
          Length = 535

 Score =  718 bits (1853), Expect = 0.0
 Identities = 358/414 (86%), Positives = 374/414 (90%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            VIVDMMN+NR+SIDVESETA VESGATLGELYYAIAQSTETLGFTAGWCPTV        
Sbjct: 122  VIVDMMNLNRISIDVESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVGSGGHISG 181

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILIDS G+ILDREKMGEDVFWAIR             KIK
Sbjct: 182  GGFGMMSRKYGLAADNVVDAILIDSTGSILDREKMGEDVFWAIRGGGGGVWGAVYAWKIK 241

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEKLTVFRVTKNVGIE ASSLLHKWQYVADELD DFTVSVLGGVNGN+AWLMFLGL
Sbjct: 242  LLPVPEKLTVFRVTKNVGIEGASSLLHKWQYVADELDEDFTVSVLGGVNGNDAWLMFLGL 301

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            +LGR+DAAK+II+EKFPELG VE+EFQEMSW AS AFL+GLET+SELNNRFLKFD+RAFK
Sbjct: 302  YLGREDAAKTIIDEKFPELGSVEEEFQEMSWVASTAFLSGLETVSELNNRFLKFDERAFK 361

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVDFTKE VPL+VFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE
Sbjct: 362  TKVDFTKEPVPLNVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 421

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YIIAWNQDEETKI EFSEWL KFYDYLEPFVSK PRVGYVNHIDL+IGGIDW+NKSSTSN
Sbjct: 422  YIIAWNQDEETKIEEFSEWLTKFYDYLEPFVSKGPRVGYVNHIDLEIGGIDWRNKSSTSN 481

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEENYILNEL 1243
            AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEENY+L EL
Sbjct: 482  AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEENYMLKEL 535


>gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]
          Length = 554

 Score =  612 bits (1578), Expect = e-172
 Identities = 290/405 (71%), Positives = 340/405 (83%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            V++D+MN+NR+SID++SETA VESGATLGELYYAI + T++LGFTAGWCPTV        
Sbjct: 130  VLIDLMNLNRISIDIDSETAWVESGATLGELYYAITELTDSLGFTAGWCPTVGSGGHISG 189

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNV D ILIDSKGAILDR+ MGEDVFWA+R             KIK
Sbjct: 190  GGFGMMSRKYGLAADNVEDVILIDSKGAILDRKLMGEDVFWAVRGGGGGVWGAIYAWKIK 249

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVP+K+TVFRVTKNV IE AS L+HKWQYVADELD+DFTVS+LGG NGNE W++FLGL
Sbjct: 250  LLPVPKKVTVFRVTKNVNIEEASFLIHKWQYVADELDDDFTVSILGGANGNEVWVIFLGL 309

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            HLG K  AKSII++KFPELGL+E+EF EM+WG S A+L+GL+T+ ELNNRFLKFDDRAFK
Sbjct: 310  HLGCKTVAKSIIDKKFPELGLIEEEFLEMNWGESFAYLSGLKTVKELNNRFLKFDDRAFK 369

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVDFTKE +PL+     LE+LS++P GFIALNGFGGKMS+IS DFTPFPHRKGTKLM E
Sbjct: 370  TKVDFTKETLPLEAIDGLLEILSKEPRGFIALNGFGGKMSKISNDFTPFPHRKGTKLMVE 429

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AW++DEE+K  EF +WL   YDY+E FVSK PRVGYVNHIDLD+GGIDW +K+S++N
Sbjct: 430  YIVAWSKDEESKSDEFFDWLRNIYDYMEMFVSKNPRVGYVNHIDLDLGGIDWSDKNSSNN 489

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKF 1216
            A+EIARNWGE+YF SNYERL++AKTLIDPNNVFNHPQSIPPMMK+
Sbjct: 490  AIEIARNWGEKYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMMKY 534


>gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]
          Length = 536

 Score =  592 bits (1526), Expect = e-166
 Identities = 279/406 (68%), Positives = 334/406 (82%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            V++D+MN+NR+SID++SETA VESGAT+GELYYAI++ST++ GFTAGWCPTV        
Sbjct: 125  VLIDLMNLNRISIDMDSETAWVESGATVGELYYAISESTDSFGFTAGWCPTVGTGGHISG 184

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNV D ILIDS GAILDR+ MGEDVFWA+R             KIK
Sbjct: 185  GGFGMMSRKYGLAADNVEDVILIDSNGAILDRKLMGEDVFWAVRGGGGGVWGAIYAWKIK 244

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVP+K+TVFRV KNV IE AS L+HKWQYVADELD+DFTV++LGG NGN AWL+FLGL
Sbjct: 245  LLPVPKKVTVFRVMKNVNIEEASFLIHKWQYVADELDDDFTVTILGGANGNGAWLVFLGL 304

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            HLG K  AKSI+++ FPELGL+E+EF EM+WG S A+L+GL+T+ ELNNRFLK DD+AFK
Sbjct: 305  HLGCKTVAKSIMDKMFPELGLIEEEFLEMNWGESFAYLSGLKTVKELNNRFLKLDDKAFK 364

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVDFTKE +PL V    LE+LS++P GFI LN  GGKMS+IS DFTPFPHR GTKLM E
Sbjct: 365  TKVDFTKETLPLKVINGVLEILSKEPRGFILLNSLGGKMSKISNDFTPFPHRNGTKLMVE 424

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI++W++DEE+K  EF +WL   YDY+E FVSK PRVGYVN+IDLD+GGIDW NK+S++N
Sbjct: 425  YIVSWSKDEESKSDEFFDWLRNIYDYMEEFVSKNPRVGYVNNIDLDLGGIDWSNKNSSNN 484

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFE 1219
            A+EIARNWGE+YF SNYERL++AKTLIDPNN+FNHPQSIPPMMKF+
Sbjct: 485  AIEIARNWGEKYFLSNYERLIRAKTLIDPNNIFNHPQSIPPMMKFD 530


>sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName: Full=Berberine
            bridge-forming enzyme; Short=BBE; AltName:
            Full=Tetrahydroprotoberberine synthase; Flags: Precursor
            gi|239110|gb|AAB20352.1| (S)-reticuline:oxygen
            oxidoreductase (methylene-bridge-forming) [Eschscholzia
            californica] gi|2897944|gb|AAC39358.1| berberine bridge
            enzyme [Eschscholzia californica]
          Length = 538

 Score =  572 bits (1475), Expect = e-160
 Identities = 273/406 (67%), Positives = 325/406 (80%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            +++D+MN+NRVSID+ESETA VESG+TLGELYYAI +S+  LGFTAGWCPTV        
Sbjct: 118  ILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISG 177

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR             KIK
Sbjct: 178  GGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIK 237

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SVLGG +  + WL  LG 
Sbjct: 238  LLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSVLGGADEKQVWLTMLGF 297

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            H G K  AKS  +  FPELGLVE+++ EMSWG S A+LAGLET+S+LNNRFLKFD+RAFK
Sbjct: 298  HFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETVSQLNNRFLKFDERAFK 357

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR GT+LM E
Sbjct: 358  TKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVE 417

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW NK+  +N
Sbjct: 418  YIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNN 477

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFE 1219
            A+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM  F+
Sbjct: 478  AIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMANFD 523


>pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In
            Complex With (S)-Reticuline
          Length = 519

 Score =  572 bits (1475), Expect = e-160
 Identities = 273/406 (67%), Positives = 325/406 (80%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            +++D+MN+NRVSID+ESETA VESG+TLGELYYAI +S+  LGFTAGWCPTV        
Sbjct: 99   ILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGAISG 158

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR             KIK
Sbjct: 159  GGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIK 218

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SVLGG +  + WL  LG 
Sbjct: 219  LLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSVLGGADEKQVWLTMLGF 278

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            H G K  AKS  +  FPELGLVE+++ EMSWG S A+LAGLET+S+LNNRFLKFD+RAFK
Sbjct: 279  HFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETVSQLNNRFLKFDERAFK 338

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR GT+LM E
Sbjct: 339  TKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVE 398

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW NK+  +N
Sbjct: 399  YIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNN 458

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFE 1219
            A+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM  F+
Sbjct: 459  AIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMANFD 504


>pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
            Dehydroscoulerine
          Length = 519

 Score =  572 bits (1475), Expect = e-160
 Identities = 273/406 (67%), Positives = 325/406 (80%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            +++D+MN+NRVSID+ESETA VESG+TLGELYYAI +S+  LGFTAGWCPTV        
Sbjct: 99   ILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISG 158

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR             KIK
Sbjct: 159  GGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIK 218

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SVLGG +  + WL  LG 
Sbjct: 219  LLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSVLGGADEKQVWLTMLGF 278

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            H G K  AKS  +  FPELGLVE+++ EMSWG S A+LAGLET+S+LNNRFLKFD+RAFK
Sbjct: 279  HFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETVSQLNNRFLKFDERAFK 338

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR GT+LM E
Sbjct: 339  TKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVE 398

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW NK+  +N
Sbjct: 399  YIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNN 458

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFE 1219
            A+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM  F+
Sbjct: 459  AIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMANFD 504


>pdb|3D2D|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
            (S)-Reticuline gi|210060956|pdb|3D2H|A Chain A, Structure
            Of Berberine Bridge Enzyme From Eschscholzia Californica,
            Monoclinic Crystal Form gi|210060957|pdb|3D2J|A Chain A,
            Structure Of Berberine Bridge Enzyme From Eschscholzia
            Californica, Tetragonal Crystal Form
          Length = 538

 Score =  572 bits (1475), Expect = e-160
 Identities = 273/406 (67%), Positives = 325/406 (80%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            +++D+MN+NRVSID+ESETA VESG+TLGELYYAI +S+  LGFTAGWCPTV        
Sbjct: 118  ILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISG 177

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR             KIK
Sbjct: 178  GGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIK 237

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SVLGG +  + WL  LG 
Sbjct: 238  LLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSVLGGADEKQVWLTMLGF 297

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            H G K  AKS  +  FPELGLVE+++ EMSWG S A+LAGLET+S+LNNRFLKFD+RAFK
Sbjct: 298  HFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETVSQLNNRFLKFDERAFK 357

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR GT+LM E
Sbjct: 358  TKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVE 417

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW NK+  +N
Sbjct: 418  YIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNN 477

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFE 1219
            A+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM  F+
Sbjct: 478  AIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMANFD 523


>pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
            (S)-Scoulerine
          Length = 495

 Score =  570 bits (1468), Expect = e-160
 Identities = 272/402 (67%), Positives = 323/402 (80%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            +++D+MN+NRVSID+ESETA VESG+TLGELYYAI +S+  LGFTAGWCPTV        
Sbjct: 93   ILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISG 152

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR             KIK
Sbjct: 153  GGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIK 212

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SVLGG +  + WL  LG 
Sbjct: 213  LLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSVLGGADEKQVWLTMLGF 272

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            H G K  AKS  +  FPELGLVE+++ EMSWG S A+LAGLET+S+LNNRFLKFD+RAFK
Sbjct: 273  HFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETVSQLNNRFLKFDERAFK 332

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR GT+LM E
Sbjct: 333  TKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVE 392

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW NK+  +N
Sbjct: 393  YIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNN 452

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 1207
            A+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM
Sbjct: 453  AIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPM 494


>pdb|3FW7|A Chain A, Structure Of Berberine Bridge Enzyme, H104a Variant
          Length = 498

 Score =  570 bits (1468), Expect = e-160
 Identities = 272/402 (67%), Positives = 323/402 (80%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            +++D+MN+NRVSID+ESETA VESG+TLGELYYAI +S+  LGFTAGWCPTV        
Sbjct: 96   ILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISG 155

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR             KIK
Sbjct: 156  GGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIK 215

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SVLGG +  + WL  LG 
Sbjct: 216  LLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSVLGGADEKQVWLTMLGF 275

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            H G K  AKS  +  FPELGLVE+++ EMSWG S A+LAGLET+S+LNNRFLKFD+RAFK
Sbjct: 276  HFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETVSQLNNRFLKFDERAFK 335

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR GT+LM E
Sbjct: 336  TKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVE 395

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW NK+  +N
Sbjct: 396  YIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNN 455

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 1207
            A+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM
Sbjct: 456  AIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPM 497


>pdb|3FWA|A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant In
            Complex With (S)-Reticuline
          Length = 497

 Score =  568 bits (1464), Expect = e-159
 Identities = 272/405 (67%), Positives = 323/405 (79%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            +++D+MN+NRVSID+ESETA VESG+TLGELYYAI +S+  LGFTAGW PTV        
Sbjct: 93   ILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWAPTVGTGGHISG 152

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR             KIK
Sbjct: 153  GGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIK 212

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SVLGG +  + WL  LG 
Sbjct: 213  LLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSVLGGADEKQVWLTMLGF 272

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            H G K  AKS  +  FPELGLVE+++ EMSWG S A+LAGLET+S+LNNRFLKFD+RAFK
Sbjct: 273  HFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETVSQLNNRFLKFDERAFK 332

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR GT+LM E
Sbjct: 333  TKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVE 392

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW NK+  +N
Sbjct: 393  YIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNN 452

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKF 1216
            A+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM  F
Sbjct: 453  AIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMANF 497


>pdb|3FW8|A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant
          Length = 495

 Score =  566 bits (1459), Expect = e-159
 Identities = 271/402 (67%), Positives = 322/402 (80%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            +++D+MN+NRVSID+ESETA VESG+TLGELYYAI +S+  LGFTAGW PTV        
Sbjct: 93   ILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWAPTVGTGGHISG 152

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR             KIK
Sbjct: 153  GGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIK 212

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SVLGG +  + WL  LG 
Sbjct: 213  LLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSVLGGADEKQVWLTMLGF 272

Query: 542  HLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            H G K  AKS  +  FPELGLVE+++ EMSWG S A+LAGLET+S+LNNRFLKFD+RAFK
Sbjct: 273  HFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETVSQLNNRFLKFDERAFK 332

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR GT+LM E
Sbjct: 333  TKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVE 392

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW NK+  +N
Sbjct: 393  YIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNN 452

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 1207
            A+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM
Sbjct: 453  AIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPM 494


>gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera]
          Length = 536

 Score =  491 bits (1264), Expect = e-136
 Identities = 233/403 (57%), Positives = 304/403 (75%), Gaps = 1/403 (0%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            V++D+MN+NR+SID+ES+TA VESGATLGE+Y AI+++++TLGF+ G+CPTV        
Sbjct: 114  VVIDLMNLNRISIDLESKTAWVESGATLGEIYCAISEASDTLGFSGGYCPTVGSGGHISG 173

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNV+DA+++D+ GA+LDR  MGEDVFWAIR             K++
Sbjct: 174  GGFGMMSRKYGLAADNVIDALIVDANGAVLDRSSMGEDVFWAIRGGGGGVWGAIYAWKLQ 233

Query: 362  LLPVPEKLTVFRVTKNV-GIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLG 538
            LLPVP+++TVF++ KN   IE AS +LHKWQ VA  L++DFT+SVL G + N  W  FLG
Sbjct: 234  LLPVPKQVTVFKLMKNFDNIEEASKMLHKWQVVAPALEDDFTLSVLAGADTNGIWFSFLG 293

Query: 539  LHLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAF 718
            L+LG K+ A S +++ FPEL LV ++ +EMSW  S A LAGL ++ E+NNRFLK+DDRAF
Sbjct: 294  LYLGPKELAISSVDQNFPELNLVMEDCKEMSWVESFAHLAGLNSVEEMNNRFLKYDDRAF 353

Query: 719  KTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMF 898
            KTKVDF KE +PL+  + AL ML+++  GF+A NG GG MS IS+D TPFPHRKGT +M 
Sbjct: 354  KTKVDFVKEPIPLEGIKGALTMLTKELRGFMAFNGQGGLMSRISSDSTPFPHRKGTLMMM 413

Query: 899  EYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTS 1078
            EYI+AW++DE+ K  EF  WL  FY+Y+  F+  +PR+ YVNH+DLD+G +DW N +  S
Sbjct: 414  EYIVAWDRDEDAKSYEFIGWLHGFYNYMGQFLPSDPRIAYVNHVDLDLGRLDWTNSTIAS 473

Query: 1079 NAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 1207
            NA+EIAR WGE+YF SNYERLV+AKTLIDP NVF+HPQSIPPM
Sbjct: 474  NAIEIARTWGEKYFLSNYERLVRAKTLIDPKNVFHHPQSIPPM 516


>gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp. glaucum]
          Length = 535

 Score =  471 bits (1213), Expect = e-130
 Identities = 228/421 (54%), Positives = 311/421 (73%), Gaps = 7/421 (1%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            VI+D+MN+N +SID++++TA VESGATLGE+Y+AI +S+ T+ F+AG+CPT         
Sbjct: 115  VIIDLMNLNGISIDLDTQTAWVESGATLGEIYHAIGKSSGTMAFSAGYCPTGGSGGHIAP 174

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDA+L+D+ G +LDRE MGEDVFWAIR             K++
Sbjct: 175  GGFGMMSRKYGLAADNVVDALLVDANGVVLDRESMGEDVFWAIRGGGGGVWGAVYAWKLQ 234

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEA-WLMFLG 538
            L+PVP+ +T+FR+ K+  +E AS LLHKWQ VA +L++DF+++VL G N + + WL FLG
Sbjct: 235  LVPVPKNVTIFRLMKHSEVEDASKLLHKWQLVAPKLEDDFSLAVLAGTNKDSSIWLTFLG 294

Query: 539  LHLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAF 718
            L+LG K+ A S +++KFPEL L+ ++  EMSW  + A LAGL+++SEL +RFL++DDRAF
Sbjct: 295  LYLGPKELASSSMHKKFPELNLLLEDCMEMSWVEATAELAGLKSVSELKDRFLRYDDRAF 354

Query: 719  KTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMF 898
            KTKVDF KEA+PL+  + ALE+L ++  GF+ +NG GG M  ISTD +PFPHR GT  M 
Sbjct: 355  KTKVDFPKEAIPLEGIQGALEILKKEQRGFMVMNGQGGMMDRISTDASPFPHRSGTLSMV 414

Query: 899  EYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKS-ST 1075
            EYI+AW++ E+    EF  WL + +DY+  FVS  PRVGYVNH+DLD+G IDW NK+ S+
Sbjct: 415  EYIVAWDKHEDLHSNEFIHWLHQLFDYMGKFVSNNPRVGYVNHVDLDLGRIDWVNKTISS 474

Query: 1076 SNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKF-----EENYILNE 1240
              A+EIAR WGE+YF SNY+RLV+AKT+IDP NVFNHPQSIPP+++      +EN ++ +
Sbjct: 475  GRAIEIARTWGEKYFMSNYDRLVRAKTMIDPKNVFNHPQSIPPLLEIMLDNVKENDVIYK 534

Query: 1241 L 1243
            L
Sbjct: 535  L 535


>dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica]
          Length = 533

 Score =  461 bits (1187), Expect = e-127
 Identities = 227/404 (56%), Positives = 290/404 (71%), Gaps = 2/404 (0%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            VI+DMMN+N VSID++++TA  ESGATLGE+Y+AI  S++ LGF+AG+CPTV        
Sbjct: 118  VIIDMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSSDVLGFSAGYCPTVGSGGHISG 177

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILI + GA+ DR+ MGEDVFWAIR             K++
Sbjct: 178  GGFGMMSRKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIRGGGGGVWGVVYAWKLQ 237

Query: 362  LLPVPEKLTVFRVTKNVG-IEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLG 538
            LLPVP+ +TVF++TK+   I+ AS LL+KWQ VA  LD+DF+++VL G   +  WL FLG
Sbjct: 238  LLPVPKHVTVFKLTKHTSEIDEASKLLYKWQLVAPNLDDDFSLAVLNGAEKDGFWLTFLG 297

Query: 539  LHLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAF 718
            L+LG K+ A S +++KFPEL L+ +E +E+SW  + A LAGL+ + ELNNRFLK+DDRAF
Sbjct: 298  LYLGPKEVAVSSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAF 357

Query: 719  KTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMF 898
            KTKVDF +  +PL+    AL++L ++  GF+ +NG GG M  IS D  PFPHR G   M 
Sbjct: 358  KTKVDFAEVPIPLEGINGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMI 417

Query: 899  EYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKS-ST 1075
            EYI+AW+ DE+    E+  WL +FYDY+  FV   PRVGYVNH+D D G IDW N S S 
Sbjct: 418  EYIVAWDMDEDFNSHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNSSISA 477

Query: 1076 SNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 1207
            S A+EIAR WGE+YF SNY+RLV AKTLIDPNNVF+HPQSIPP+
Sbjct: 478  SKAIEIARTWGEKYFLSNYDRLVGAKTLIDPNNVFSHPQSIPPL 521


>ref|XP_003634619.1| PREDICTED: LOW QUALITY PROTEIN: reticuline oxidase-like [Vitis
            vinifera]
          Length = 712

 Score =  360 bits (923), Expect = 1e-96
 Identities = 186/411 (45%), Positives = 268/411 (65%), Gaps = 9/411 (2%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            VI+DMM++N+VS+DVESETA VE GATLGE YYA+A+++   GF+AG CPTV        
Sbjct: 246  VIIDMMSLNQVSVDVESETAWVEGGATLGETYYAVAEASNVHGFSAGSCPTVGVGGHIAG 305

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDA+LID+ G +LDR+ MGEDVFWAIR             KIK
Sbjct: 306  GGFGLLSRKYGLAADNVVDALLIDADGRVLDRKAMGEDVFWAIRGGGGGDWGIVYAWKIK 365

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVL--GGVNGNE----AW 523
            LL VPE +T   +++       + L+HKWQ++A  L+  F +SV    G+ G +      
Sbjct: 366  LLKVPETVTSCIMSRTRTKLHVAELVHKWQFIAPRLEPSFYLSVFVGAGLQGVDEETGVS 425

Query: 524  LMFLGLHLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGL---ETISELNNRF 694
              F G +LG ++ A SI+N  FPELG+ +++ +EMSW  S+ + +GL    +ISEL NR+
Sbjct: 426  ASFKGFYLGSRNEAMSILNRVFPELGVEKEDCREMSWIESILYFSGLPNGSSISELRNRY 485

Query: 695  LKFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPH 874
            L+ D   FK K D+ +  + ++    AL++L  +P G + L+ +GG+M +IS+D  PFPH
Sbjct: 486  LE-DKLYFKAKSDYVRTPISMEGLVTALDILEMEPKGSVVLDPYGGEMEKISSDALPFPH 544

Query: 875  RKGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGID 1054
            RKG     +Y++AW +D      ++ +W+  FY ++ P+VS+ PR  YVN++DLD+G ++
Sbjct: 545  RKGNLFSIQYMVAWEEDSTAMSNKYIDWIRGFYKWMMPYVSQGPRAAYVNYMDLDLGQMN 604

Query: 1055 WKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 1207
              +  S+++ VE AR+WGE+YF +NY+RLVK KT IDP+NVFN+ Q IPPM
Sbjct: 605  --SSISSNDPVEAARDWGEKYFLNNYDRLVKVKTCIDPDNVFNNQQGIPPM 653


>ref|XP_003555533.1| PREDICTED: reticuline oxidase-like [Glycine max]
          Length = 550

 Score =  357 bits (917), Expect = 5e-96
 Identities = 186/411 (45%), Positives = 262/411 (63%), Gaps = 9/411 (2%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            VI+DMMN+N V +D+E+ETA VE GATLGE YYAI+Q +   GF+ G CPTV        
Sbjct: 129  VIIDMMNLNHVWVDMETETAWVEGGATLGETYYAISQESNEHGFSGGSCPTVGVGGHIGG 188

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDA+L+D+ G + DRE MGEDVFWAIR             KI+
Sbjct: 189  GGFGLLSRKYGLAADNVVDALLVDANGKLFDRETMGEDVFWAIRGGGGGLWGIIYAWKIQ 248

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEA-----WL 526
            +L VP+ +T F V++       ++L+HKWQYVA  L++DF +S L G    +A       
Sbjct: 249  VLKVPQVVTSFTVSRTGTKSHVANLVHKWQYVAPNLEDDFYLSCLVGAGLPQAKTTGLST 308

Query: 527  MFLGLHLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLE---TISELNNRFL 697
             F G +LG + +A SI+N+ FPEL + E+E  EMSW  S+ F +GL    ++S+L NR+L
Sbjct: 309  TFNGFYLGPRASATSILNQAFPELSIAEEECIEMSWIQSIVFFSGLSDGASVSDLKNRYL 368

Query: 698  KFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHR 877
            + +   FK K D+ K+ VPL     AL++L ++P G++ L+ +GG M  IS++   FPHR
Sbjct: 369  Q-EKEYFKAKSDYVKKNVPLVGIETALDILEKEPKGYVVLDPYGGMMHNISSESIAFPHR 427

Query: 878  KGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDW 1057
            +G     +Y+I W + +  K  ++ +W+  FY  + PFVS  PR  Y+N++D D+G ++ 
Sbjct: 428  RGNLFTIQYLIYWKEADNDKSSDYVDWIRGFYAAMTPFVSWGPRAAYINYMDFDLGVMEG 487

Query: 1058 -KNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 1207
              N ++  +AVE AR WGE+YF SNY+RLV+AKTLIDPNNVF + Q IPP+
Sbjct: 488  IGNGANMKDAVEHARVWGEKYFLSNYDRLVRAKTLIDPNNVFTNDQGIPPI 538


>ref|XP_007143918.1| hypothetical protein PHAVU_007G113300g [Phaseolus vulgaris]
            gi|561017108|gb|ESW15912.1| hypothetical protein
            PHAVU_007G113300g [Phaseolus vulgaris]
          Length = 550

 Score =  357 bits (916), Expect = 7e-96
 Identities = 184/411 (44%), Positives = 259/411 (63%), Gaps = 9/411 (2%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            VI+DMMN+N V +D+E+ETA VE GATLGE YYAI+Q++   GF+AG CPTV        
Sbjct: 129  VIIDMMNLNHVWVDMETETAWVEGGATLGETYYAISQASNEYGFSAGSCPTVGVGGHIGG 188

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDA+L+D+   +LDRE MGEDVFWAIR             KI+
Sbjct: 189  GGFGLLSRKYGLAADNVVDALLVDADQNLLDRETMGEDVFWAIRGGGGGLWGIIYAWKIQ 248

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEA-----WL 526
            LL VP+ +T F V++       ++L+HKWQYVA  L++DF +S   G    +A      L
Sbjct: 249  LLKVPQVVTSFTVSRTGTKSHVANLVHKWQYVAPNLEDDFYLSCFVGAGLPQAKTKGLSL 308

Query: 527  MFLGLHLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLE---TISELNNRFL 697
               G +LG K  A SI++  FPELG+ E+E  EMSW  S+ F +GL    ++S+L NR++
Sbjct: 309  TLNGFYLGPKTDAISILHHAFPELGVTEEECIEMSWIQSIVFFSGLSDGASVSDLKNRYV 368

Query: 698  KFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHR 877
            + +   FK K D+ K  +PL     AL++L ++P G++ L+ +GGKM  IS D   FPHR
Sbjct: 369  Q-EKEYFKAKSDYVKNHIPLVGIETALDILEKEPKGYVILDAYGGKMHNISNDAIAFPHR 427

Query: 878  KGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIG-GID 1054
            +G     +Y+I W + ++ K  ++ +W+  FYD + PFVS  PR  Y+N++D D+G    
Sbjct: 428  RGNLFTIQYLIYWKEADKEKSSDYVDWIRGFYDAMSPFVSSAPRAAYINYMDFDLGVRKR 487

Query: 1055 WKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 1207
             ++     + VE AR+WGE+YF SNY+RLV+ KT++DPNNVF + Q IPP+
Sbjct: 488  IRSYGDVEDGVENARDWGEKYFLSNYDRLVRVKTMVDPNNVFTNEQGIPPL 538


>ref|XP_003535410.1| PREDICTED: reticuline oxidase-like [Glycine max]
          Length = 550

 Score =  353 bits (907), Expect = 8e-95
 Identities = 186/411 (45%), Positives = 262/411 (63%), Gaps = 9/411 (2%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            VI+DMMN+N V +D+E+ETA VE GATLGE YYAI+Q++   GF+ G CPTV        
Sbjct: 129  VIIDMMNLNHVWVDMETETAWVEGGATLGETYYAISQASNEHGFSGGSCPTVGVGGHIGG 188

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDA+L+++ G + DRE MGEDVFWAIR             KIK
Sbjct: 189  GGFGILSRKYGLAADNVVDALLVNADGKLFDRETMGEDVFWAIRGGGGGLWGIIYAWKIK 248

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEA-----WL 526
            +L +P+ +T F V++       ++L+HKWQ VA  L++DF +S   G    +A       
Sbjct: 249  VLKLPQVVTSFTVSRTGTKRHVANLVHKWQNVAPNLEDDFYLSCFVGAGLPQAKTKGLST 308

Query: 527  MFLGLHLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGLE---TISELNNRFL 697
             F G +LG +  A SI++  FPELG+VE+E  EMSW  S  F +GL    ++S+LNNR+L
Sbjct: 309  TFNGFYLGPRAGAISILDHAFPELGIVEEECIEMSWIQSTVFFSGLSDGASVSDLNNRYL 368

Query: 698  KFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHR 877
            + + + FK K D+ K+ VPL     AL++L ++P G++ L+ +GGKM  IS++   FPHR
Sbjct: 369  Q-EKQYFKAKSDYVKKHVPLVGIETALDILEKEPKGYVILDPYGGKMHNISSESIAFPHR 427

Query: 878  KGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGID- 1054
            +G     +Y+I W + +  K  ++ +W+  FY  + PFVS  PR  YVN++D D+G ++ 
Sbjct: 428  RGNLFTIQYLIYWKEADNDKNSDYVDWIRGFYAAMTPFVSWGPRAAYVNYMDFDLGVMER 487

Query: 1055 WKNKSSTSNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 1207
              N ++  + VE AR WGE+YF SNY+RLV+AKTLIDPNNVF + Q IPP+
Sbjct: 488  ISNGANMKDVVEHARVWGEKYFLSNYDRLVRAKTLIDPNNVFTNDQGIPPI 538


>ref|XP_002533924.1| d-lactate dehydrogenase, putative [Ricinus communis]
            gi|223526119|gb|EEF28466.1| d-lactate dehydrogenase,
            putative [Ricinus communis]
          Length = 546

 Score =  353 bits (907), Expect = 8e-95
 Identities = 186/411 (45%), Positives = 264/411 (64%), Gaps = 9/411 (2%)
 Frame = +2

Query: 2    VIVDMMNMNRVSIDVESETALVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            V++DMMN+N+VS+D+E++ A+VE GATLGE Y AIA+++   GF+AG CPTV        
Sbjct: 126  VVIDMMNLNKVSVDLEAKMAVVEGGATLGETYAAIAEASGVHGFSAGSCPTVGVGGHIGG 185

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDA+L+D+ G +LDR++MGEDVFWAIR             KIK
Sbjct: 186  GGFGLLSRKYGLAADNVVDALLVDANGRLLDRKEMGEDVFWAIRGGGGGVWGILYAWKIK 245

Query: 362  LLPVPEKLTVFRVTKNVGIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEA-----WL 526
            LL VP  +T F V++       + L++KWQ+VA  LD+DF +S   G    EA       
Sbjct: 246  LLKVPRVVTGFIVSRPGTKGHVAKLVNKWQHVAPGLDDDFYLSCFVGAGLPEAKTTGISA 305

Query: 527  MFLGLHLGRKDAAKSIINEKFPELGLVEKEFQEMSWGASMAFLAGL---ETISELNNRFL 697
             F G +LG +  A SI+N+ FPELG+VE++ +EMSW  S+ F +GL    T+S+L NR L
Sbjct: 306  TFKGFYLGPRSEAVSILNKNFPELGIVEEDCKEMSWIESVLFFSGLSNGSTVSDLKNRHL 365

Query: 698  KFDDRAFKTKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHR 877
            +     FK K D+ K  +     + AL++L ++P G++ L+ +GG M  IS++   FPHR
Sbjct: 366  Q-GKSYFKAKSDYVKSEISSAGIKIALDILQKEPKGYVILDPYGGVMHNISSEAIAFPHR 424

Query: 878  KGTKLMFEYIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDW 1057
             G     +Y++ W + ++ K   + +W+ +FY  + PFVS+ PR  Y+N++D DIG +  
Sbjct: 425  NGNLFTIQYLVEWKEKDKNKSNVYIDWIREFYYLMTPFVSRGPRAAYINYMDFDIGAMGM 484

Query: 1058 KNKSSTS-NAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 1207
              KS  S +AVEIAR WGE+YF  N++RLV+AKTLIDP+NVFN+ QSIPP+
Sbjct: 485  IKKSVPSEDAVEIARVWGEKYFLRNFDRLVRAKTLIDPDNVFNNEQSIPPL 535