BLASTX nr result
ID: Papaver25_contig00001689
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver25_contig00001689
(854 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 494 e-137
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 494 e-137
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 490 e-136
gb|AFK73713.1| cytochrome P450 [Papaver somniferum] 439 e-121
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 409 e-112
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 406 e-111
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 371 e-100
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 364 3e-98
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 352 1e-94
gb|ACM44075.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver rh... 243 9e-62
gb|ACM44072.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver br... 241 3e-61
gb|ACM44071.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver so... 240 6e-61
gb|ACM44073.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver nu... 235 2e-59
ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 202 2e-49
ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 198 2e-48
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 196 7e-48
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 196 7e-48
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera] 196 9e-48
ref|XP_006358915.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 195 2e-47
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 192 1e-46
>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 494 bits (1271), Expect = e-137
Identities = 250/269 (92%), Positives = 256/269 (95%)
Frame = -2
Query: 808 SLVAVVITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTL 629
SLVAVVITTFLYLIFRDSS PIVGNLLQLGEKPHSQFA+LA+TYGDLF+L
Sbjct: 1 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSL 60
Query: 628 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 449
KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK
Sbjct: 61 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 120
Query: 448 VCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 269
VCRTELFTQKMIESQAE+RE KAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN
Sbjct: 121 VCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 180
Query: 268 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYNVW 89
IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLG+FDLFGQ KDVADCLQGIY+VW
Sbjct: 181 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240
Query: 88 GAMLKERKIAKQHNNSKKNDFVEILLDSG 2
GAMLKERKIAKQHNNSKKNDFVEILLDSG
Sbjct: 241 GAMLKERKIAKQHNNSKKNDFVEILLDSG 269
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 494 bits (1271), Expect = e-137
Identities = 252/272 (92%), Positives = 256/272 (94%)
Frame = -2
Query: 817 VTVSLVAVVITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDL 638
VTVSLVAVVITTFLYLIFRDSS PIVGNLLQLGEKPHSQFAELAKTYGDL
Sbjct: 3 VTVSLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDL 62
Query: 637 FTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKK 458
FTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKK
Sbjct: 63 FTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKK 122
Query: 457 LRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIF 278
LRKVCR ELFTQKMIESQAEIRE KAMEMVE+LK+N G+EVKI EVVFGTLVNIFGNLIF
Sbjct: 123 LRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIF 182
Query: 277 SQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIY 98
SQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIY
Sbjct: 183 SQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIY 242
Query: 97 NVWGAMLKERKIAKQHNNSKKNDFVEILLDSG 2
+VWGAMLKERKIAK HNNSKKNDFVEILLDSG
Sbjct: 243 SVWGAMLKERKIAKLHNNSKKNDFVEILLDSG 274
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 490 bits (1262), Expect = e-136
Identities = 248/269 (92%), Positives = 255/269 (94%)
Frame = -2
Query: 808 SLVAVVITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTL 629
SLVAVVITTFLYLIFRDSS PIVGNLLQLGEKPHSQFA+LA+TYGDLF+L
Sbjct: 1 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSL 60
Query: 628 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 449
KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK
Sbjct: 61 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 120
Query: 448 VCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 269
VCRT+LFTQKMIESQAE+RE KAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN
Sbjct: 121 VCRTDLFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 180
Query: 268 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYNVW 89
IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLG+FDLFGQ KDVADCLQGIY+VW
Sbjct: 181 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240
Query: 88 GAMLKERKIAKQHNNSKKNDFVEILLDSG 2
GAMLKE KIAKQHNNSKKNDFVEILLDSG
Sbjct: 241 GAMLKESKIAKQHNNSKKNDFVEILLDSG 269
>gb|AFK73713.1| cytochrome P450 [Papaver somniferum]
Length = 440
Score = 439 bits (1130), Expect = e-121
Identities = 218/276 (78%), Positives = 242/276 (87%), Gaps = 1/276 (0%)
Frame = -2
Query: 826 MEIVTVSLVAVVITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTY 647
MEIVTV+L+A+V TTFLYLI R+SS PIVGNLLQLGEKPHSQFA+LA+TY
Sbjct: 1 MEIVTVALIAIVFTTFLYLIVRESSPNGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETY 60
Query: 646 GDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNET 467
GDLFTLKLG++TVVVASTPLAASE+LK HDR L GRYVFQSFRVK HVENSIVW+ECNET
Sbjct: 61 GDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNET 120
Query: 466 WKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 287
WKKLRKVCRT+LFTQKMIE+QAE+RE K MEMV+YLKKN G EVKI EV+FGTLVN+FGN
Sbjct: 121 WKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGN 180
Query: 286 LIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQ 107
LIFSQ+IFKLGDESSGS+EMK+H+WRMLELGNS NP DY P LG DLFGQRKDVADCLQ
Sbjct: 181 LIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQ 240
Query: 106 GIYNVWGAMLKERKIAK-QHNNSKKNDFVEILLDSG 2
G+Y VWGAMLKER+IAK Q N KNDFV++LLDSG
Sbjct: 241 GVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSG 276
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2
gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 488
Score = 409 bits (1052), Expect = e-112
Identities = 204/276 (73%), Positives = 234/276 (84%), Gaps = 1/276 (0%)
Frame = -2
Query: 826 MEIVTVSLVAVVITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTY 647
ME+VTV+L+AV+I++ LYL+F S PIVGNLLQLGEKPH+QFAELA+TY
Sbjct: 1 MEVVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTY 60
Query: 646 GDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNET 467
GD+FTLK+G+ETVVVAST AASEILKTHDR+LS RYVFQSFRVK HVENSIVWS+C ET
Sbjct: 61 GDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTET 120
Query: 466 WKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 287
WK LRKVCRTELFTQKMIESQA +RE K EMVEYL K G EVKI EV+FGTLVNIFGN
Sbjct: 121 WKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 180
Query: 286 LIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQ 107
LIFSQNIF+LG +SGS E KE+LWRMLELGNSTNPADYFP LG+FDLFGQRK+VA+CL+
Sbjct: 181 LIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 240
Query: 106 GIYNVWGAMLKERKIAKQHNN-SKKNDFVEILLDSG 2
GIY +WGAML+ERK+AK+ + KNDFV++ LDSG
Sbjct: 241 GIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSG 276
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1
gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 487
Score = 406 bits (1044), Expect = e-111
Identities = 203/272 (74%), Positives = 232/272 (85%), Gaps = 1/272 (0%)
Frame = -2
Query: 814 TVSLVAVVITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLF 635
TV+L+AV+I++ LYL+F S PIVGNLLQLGEKPH+QFAELA+TYGD+F
Sbjct: 4 TVALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIF 63
Query: 634 TLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKL 455
TLK+G+ETVVVAST AASEILKTHDR+LS RYVFQSFRVK HVENSIVWS+C ETWK L
Sbjct: 64 TLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNL 123
Query: 454 RKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFS 275
RKVCRTELFTQKMIESQA +RE K EMVEYL K G EVKI EV+FGTLVNIFGNLIFS
Sbjct: 124 RKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFS 183
Query: 274 QNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYN 95
QNIF+LGD +SGS E KE+LWRMLELGNSTNPADYFP LG+FDLFGQRK+VA+CL+GIY
Sbjct: 184 QNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYA 243
Query: 94 VWGAMLKERKIAKQHNNSK-KNDFVEILLDSG 2
+WGAML+ERK+AK+ + K KNDFV++ LDSG
Sbjct: 244 IWGAMLQERKLAKKVDGYKSKNDFVDVCLDSG 275
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
isozyme 2; AltName: Full=Cytochrome P450 80B2
gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 371 bits (952), Expect = e-100
Identities = 181/275 (65%), Positives = 226/275 (82%)
Frame = -2
Query: 826 MEIVTVSLVAVVITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTY 647
ME++++++V+ FL+ I RDS PIVGNLLQLG+KPH++FA+LA+ Y
Sbjct: 1 MEVLSIAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 60
Query: 646 GDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNET 467
G+LF+LKLGS+TVVVAS+P AA+EILKTHD++LSGRYVFQSFRVKEHVENSIVWSECN+
Sbjct: 61 GELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120
Query: 466 WKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 287
WK LRKVCRTELFT KMIESQ+EIRE KA EMV++L+ G VKI EVVFGTLVNIFGN
Sbjct: 121 WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGN 180
Query: 286 LIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQ 107
LIFS+++F L D + GSVE+KEHLW++L++GNSTNPADYFP +G+ DLFGQR+ VA+ LQ
Sbjct: 181 LIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQ 240
Query: 106 GIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSG 2
IY+VWG MLKER+ K + KNDFV++LL++G
Sbjct: 241 QIYDVWGVMLKERRGTK--GSESKNDFVDVLLNAG 273
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 364 bits (934), Expect = 3e-98
Identities = 179/275 (65%), Positives = 222/275 (80%)
Frame = -2
Query: 826 MEIVTVSLVAVVITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTY 647
ME+V++++V+ FL+ I RD PIVGNLLQLG+KP ++FA+LA+ Y
Sbjct: 1 MEVVSIAIVSFSFLLFLFFILRDPRQKNLPPGPRPLPIVGNLLQLGDKPRAEFAKLAQKY 60
Query: 646 GDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNET 467
G LF+LKLGS+TVVVAS+P AA+E+LKTHD++LSGRYVFQSFRVKEHVENSIVWSECN+
Sbjct: 61 GQLFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120
Query: 466 WKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 287
WK LRKVCRTELFT KMIESQ+EIRE KA EMV++L+ G VKI EVVFGTLVNIFGN
Sbjct: 121 WKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGN 180
Query: 286 LIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQ 107
LIFS+++F L D + GSVE+KEHLW++LE+GNSTNPADYFP +G++DLFGQR+ VAD LQ
Sbjct: 181 LIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQ 240
Query: 106 GIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSG 2
IY+VWG LKER+ K + NDFV++LL++G
Sbjct: 241 QIYDVWGINLKERRGMK--GSESNNDFVDVLLNAG 273
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 352 bits (903), Expect = 1e-94
Identities = 175/276 (63%), Positives = 220/276 (79%), Gaps = 1/276 (0%)
Frame = -2
Query: 826 MEIVTVSLVAVV-ITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKT 650
ME+++ ++V++ I F++ I DS PIVGNLLQLGEKPH++FA+LA+
Sbjct: 1 MEVLSAAMVSLFFIFLFIFFILCDSRNKDLPPGPRPSPIVGNLLQLGEKPHAEFAKLAEK 60
Query: 649 YGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNE 470
YG+LFTLKLGS+TVVVAS+P AA+EILKT D++LSGRYVFQSFRV EHV NSIVWSECNE
Sbjct: 61 YGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNE 120
Query: 469 TWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 290
WK LRKVCRTELF+ KMIESQA IRE KA++MV +L+K EVKI EVVF TLVNIFG
Sbjct: 121 NWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFG 180
Query: 289 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 110
NLIFS+++F L D + GS E+KEHLW++L++GNSTNPADYFP +G+FDLFGQR++VA L
Sbjct: 181 NLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVL 240
Query: 109 QGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSG 2
+ IY+VWG MLKER+ H ++NDF +LL++G
Sbjct: 241 KQIYDVWGVMLKERRSITGH---RENDFANVLLNAG 273
>gb|ACM44075.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver rhoeas]
Length = 139
Score = 243 bits (619), Expect = 9e-62
Identities = 125/139 (89%), Positives = 127/139 (91%)
Frame = -2
Query: 793 VITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE 614
VITTFLYLIFRDSS PIVGNLLQLGEKPHSQFAELA+TYGDLFTLKLGSE
Sbjct: 1 VITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAQTYGDLFTLKLGSE 60
Query: 613 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 434
TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE
Sbjct: 61 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 120
Query: 433 LFTQKMIESQAEIRECKAM 377
LFTQKMIESQAE+RE KAM
Sbjct: 121 LFTQKMIESQAEVRESKAM 139
>gb|ACM44072.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
gi|222101959|gb|ACM44074.1| (S)-N-methylcoclaurine
3'-hydroxylase [Papaver orientale]
Length = 139
Score = 241 bits (615), Expect = 3e-61
Identities = 124/139 (89%), Positives = 126/139 (90%)
Frame = -2
Query: 793 VITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE 614
VITTFLYLIFRDSS PIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE
Sbjct: 1 VITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE 60
Query: 613 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 434
TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECN+TWKKLRKVCR E
Sbjct: 61 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAE 120
Query: 433 LFTQKMIESQAEIRECKAM 377
LFTQKMIESQAE+RE KAM
Sbjct: 121 LFTQKMIESQAEVRESKAM 139
>gb|ACM44071.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 139
Score = 240 bits (612), Expect = 6e-61
Identities = 123/139 (88%), Positives = 127/139 (91%)
Frame = -2
Query: 793 VITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE 614
VITTFLYLIFRDSS PIVGNLLQLGEKPHSQFA+LA+TYGDLF+LKLGSE
Sbjct: 1 VITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSE 60
Query: 613 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 434
TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE
Sbjct: 61 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 120
Query: 433 LFTQKMIESQAEIRECKAM 377
LFTQKMIESQAE+RE KAM
Sbjct: 121 LFTQKMIESQAEVRESKAM 139
>gb|ACM44073.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver nudicaule]
Length = 139
Score = 235 bits (599), Expect = 2e-59
Identities = 120/139 (86%), Positives = 124/139 (89%)
Frame = -2
Query: 793 VITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE 614
VITTFLY IFRDS+ PIVGNLLQLGEKPHSQFA+LA+TYGDLFTLKLGSE
Sbjct: 1 VITTFLYFIFRDSTPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFTLKLGSE 60
Query: 613 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 434
TVVVASTPLAASEILK HDRVLSGRYVFQSFRVK HVENSIVWSECNETWKKLRKVCRTE
Sbjct: 61 TVVVASTPLAASEILKAHDRVLSGRYVFQSFRVKNHVENSIVWSECNETWKKLRKVCRTE 120
Query: 433 LFTQKMIESQAEIRECKAM 377
LFTQKMIESQAE+RE KAM
Sbjct: 121 LFTQKMIESQAEVRESKAM 139
>ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 496
Score = 202 bits (513), Expect = 2e-49
Identities = 104/264 (39%), Positives = 172/264 (65%), Gaps = 4/264 (1%)
Frame = -2
Query: 781 FLYLIFRDSSXXXXXXXXXXXP-IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVV 605
F++ IF+ SS ++GN+ Q+G+ PH AKTYG L +L+LG++ +V
Sbjct: 21 FIFKIFKKSSKITQLPPGPTPWPVIGNIFQIGKMPHITLTNFAKTYGPLISLRLGTQCLV 80
Query: 604 VASTPLAASEILKTHDRVLSGRYVFQSFRVK--EHVENSIVW-SECNETWKKLRKVCRTE 434
V S+P A EI KTHDR+LSGR+V + R K + + S+ W SEC+ W+ LR +CRT+
Sbjct: 81 VGSSPSVAIEIFKTHDRILSGRHVPNAVRSKGSDLEKTSLGWKSECDSGWRYLRTLCRTD 140
Query: 433 LFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLG 254
LF K++ESQA +RE K ME+VE+L+ G V I E+VF T++N+ N++ S+++ L
Sbjct: 141 LFAGKVLESQACLREKKVMELVEFLRSKEGQVVNIGELVFATVLNMLSNVLISKDMIHLE 200
Query: 253 DESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLK 74
+E+ S E+K + ++E+G++ N +D++P LG+ DL G RK D + ++W +++
Sbjct: 201 EETEDS-EIKSLVRGIVEVGSAPNISDFYPILGKLDLQGLRKKSTDLVTKFCSIWEPIIE 259
Query: 73 ERKIAKQHNNSKKNDFVEILLDSG 2
ER+ ++++++S + DF+E LLD+G
Sbjct: 260 ERRNSRENDSSSQQDFLETLLDNG 283
>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223549945|gb|EEF51432.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 198 bits (504), Expect = 2e-48
Identities = 105/277 (37%), Positives = 168/277 (60%), Gaps = 4/277 (1%)
Frame = -2
Query: 820 IVTVSLVAVVITTFLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGD 641
++ + ++ V++T + I PI+GN+LQ+ +K H A AK YG
Sbjct: 10 LLPLPILVVILTQIISFISSRRQHLPLPPGPKPWPILGNILQVDKKLHICIANFAKVYGP 69
Query: 640 LFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN--SIVWSE-CNE 470
L +L+LG++ +VVASTP +A+E+LK HDR+LS R++ ++ + H+ +IVW+ CN+
Sbjct: 70 LISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHILERVAIVWNPACND 129
Query: 469 TWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 290
WK LR +CRTELF+ K IESQA +RE K EM+E+L G V +AEVVFGT+ N
Sbjct: 130 HWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEVVFGTIFNTIS 189
Query: 289 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 110
+L+FS ++ ++ G +K LW M+E+ S N A+++P L D G ++ + CL
Sbjct: 190 HLLFSTDLIGFENQIGG---VKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMTKCL 246
Query: 109 QGIYNVWGAMLKERKIAKQHNN-SKKNDFVEILLDSG 2
+ ++ VW +KER+ H++ + K DF++I L +G
Sbjct: 247 KEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNG 283
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 196 bits (499), Expect = 7e-48
Identities = 105/241 (43%), Positives = 151/241 (62%), Gaps = 3/241 (1%)
Frame = -2
Query: 715 IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRY 536
I+GNLLQ+G+ PH++ A LAK +G L +L+LG++ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 587 IIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRY 646
Query: 535 VFQSFRVKEHVEN--SIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVE 365
+ S VK N SIV++ +CN WK LR +CR ELF+ K +ESQ E+RE K E+VE
Sbjct: 647 LSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVE 706
Query: 364 YLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNST 185
+L G VK+ ++VF T+ NI N FS ++ DE +K+ + + E G +
Sbjct: 707 FLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATP 766
Query: 184 NPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDS 5
N +DY+P LG D+ G + + + I W +LKER+ ++ N S DF+E LL+
Sbjct: 767 NLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEI 825
Query: 4 G 2
G
Sbjct: 826 G 826
Score = 188 bits (477), Expect = 3e-45
Identities = 104/240 (43%), Positives = 145/240 (60%), Gaps = 4/240 (1%)
Frame = -2
Query: 715 IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRY 536
I+GNLLQLG+ PH + A LAK +G L +L+LG++ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 1092 IIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRY 1151
Query: 535 VFQSFRVKEHVENSIVWS---ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVE 365
V S VK+ N + + EC WK LR +CRTE+F+ K +ES E+RE K ME+VE
Sbjct: 1152 VSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVE 1211
Query: 364 YLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNST 185
+L G VK+ ++VF T+ NI N FS ++ E +K+ ++R ELG +
Sbjct: 1212 FLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIYRAAELGATP 1266
Query: 184 NPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHN-NSKKNDFVEILLD 8
N +D++P L +L G +K + L I W LKER+ KQ N S D +E L+
Sbjct: 1267 NLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEAFLE 1324
Score = 177 bits (449), Expect = 5e-42
Identities = 99/241 (41%), Positives = 146/241 (60%), Gaps = 3/241 (1%)
Frame = -2
Query: 715 IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRY 536
I+GNLLQ+G H + A LAK +G L +L+LG++ +VVAS+ AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGGNLHVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGRY 106
Query: 535 VFQSFRVKEHVENSIVWS---ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVE 365
V + V N + + CN W+ L+ +CR ELF+ K +ES+ E+RE K ME+VE
Sbjct: 107 VSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKVMELVE 166
Query: 364 YLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNST 185
+L+K G VK+ ++V+ T+ NI N FS + V +K+ L ELG +T
Sbjct: 167 FLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFEGRDVRGVLLKDLLNENAELG-AT 225
Query: 184 NPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDS 5
N D++P LG D+ G RK + + + I W +LKER+ + H +S DF++ LL++
Sbjct: 226 NILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRKQRIHGSS-HGDFLDALLET 284
Query: 4 G 2
G
Sbjct: 285 G 285
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 196 bits (499), Expect = 7e-48
Identities = 105/241 (43%), Positives = 151/241 (62%), Gaps = 3/241 (1%)
Frame = -2
Query: 715 IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRY 536
I+GNLLQ+G+ PH++ A LAK +G L +L+LG++ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRY 106
Query: 535 VFQSFRVKEHVEN--SIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVE 365
+ S VK N SIV++ +CN WK LR +CR ELF+ K +ESQ E+RE K E+VE
Sbjct: 107 LSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVE 166
Query: 364 YLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNST 185
+L G VK+ ++VF T+ NI N FS ++ DE +K+ + + E G +
Sbjct: 167 FLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATP 226
Query: 184 NPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDS 5
N +DY+P LG D+ G + + + I W +LKER+ ++ N S DF+E LL+
Sbjct: 227 NLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEI 285
Query: 4 G 2
G
Sbjct: 286 G 286
>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 196 bits (498), Expect = 9e-48
Identities = 105/241 (43%), Positives = 151/241 (62%), Gaps = 3/241 (1%)
Frame = -2
Query: 715 IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRY 536
I+GNLLQ+G+ PH++ A LAK +G L +L+LG++ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRY 106
Query: 535 VFQSFRVKEHVEN--SIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVE 365
+ S VK N SIV++ +CN WK LR +CR ELF+ K +ESQ E+RE K E+VE
Sbjct: 107 LSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVE 166
Query: 364 YLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNST 185
+L G VK+ ++VF T+ NI N FS ++ DE +K+ + + E G +
Sbjct: 167 FLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATP 226
Query: 184 NPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDS 5
N +DY+P LG D+ G + + + I W +LKER+ ++ N S DF+E LL+
Sbjct: 227 NLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEI 285
Query: 4 G 2
G
Sbjct: 286 G 286
>ref|XP_006358915.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 495
Score = 195 bits (496), Expect = 2e-47
Identities = 106/263 (40%), Positives = 167/263 (63%), Gaps = 3/263 (1%)
Frame = -2
Query: 781 FLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVV 602
F++ IF+ S P++GN+ Q+G+ PH AKTYG L +LKLG++ +VV
Sbjct: 21 FIFKIFKSSKITQLPPGPTPWPVIGNIFQMGKMPHITLTNFAKTYGPLMSLKLGTKWLVV 80
Query: 601 ASTPLAASEILKTHDRVLSGRYVFQSFRVK--EHVENSIVW-SECNETWKKLRKVCRTEL 431
S+PLAA EILKTHD++LSGR V + K + + S+ W SECN W+ LR +CRTEL
Sbjct: 81 GSSPLAAVEILKTHDKILSGRDVPSAAPSKGSDLDKTSLGWISECNSGWRYLRTLCRTEL 140
Query: 430 FTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGD 251
F K++ESQA +RE K ME+VE+L+ G V I E+VF T++N+ N++ S+++ L +
Sbjct: 141 FAGKVLESQACLREKKVMELVEFLRSKEGQVVNIGELVFATVLNMLSNVLISKDMVNLEE 200
Query: 250 ESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKE 71
E V +K + ++E+ ++ N +D +P LG+ DL G +K D + + + W +L+E
Sbjct: 201 EIEDGV-IKSLVRGIVEVLSAPNISDLYPILGKLDLQGLQKKSIDLVTKLCSNWEPILEE 259
Query: 70 RKIAKQHNNSKKNDFVEILLDSG 2
R+ +++ +S + DF+E LLD+G
Sbjct: 260 RRNSREKGSSSQQDFLETLLDNG 282
>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547255|gb|EEF48750.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 192 bits (488), Expect = 1e-46
Identities = 99/241 (41%), Positives = 158/241 (65%), Gaps = 3/241 (1%)
Frame = -2
Query: 715 IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRY 536
I+GN+LQ+G+ PH A AK +G L +L+LG+ VVVAS+P AA+EILKTHDR+LSGRY
Sbjct: 46 ILGNILQVGKNPHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRY 105
Query: 535 VFQSFRVKEHVEN--SIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVE 365
+ + +++V + ++VW+ C++ WK LR +CR+ELF+ K IESQA +RE K EM++
Sbjct: 106 IPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLD 165
Query: 364 YLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNST 185
+L G V I EVVF T N NL+FS+++ D+ + E+K + ++EL
Sbjct: 166 FLTSKQGQIVNIGEVVFTTAFNTISNLLFSKDLLSFEDQGNAG-ELKTLISTLMELATCP 224
Query: 184 NPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDS 5
N AD++P L + D G ++ + +CL+ ++ VW +KER+ + +++K DF+++ L +
Sbjct: 225 NIADFYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERR-ERHVKDARKTDFLDVFLSN 283
Query: 4 G 2
G
Sbjct: 284 G 284