BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver23_contig00018396
         (1356 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFK73712.1| pavine-N-methyltransferase [Papaver somniferum]        466   e-129
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      409   e-112
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   402   e-110
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   395   e-107
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   392   e-106

>gb|AFK73712.1| pavine-N-methyltransferase [Papaver somniferum]
          Length = 330

 Score =  466 bits (1198), Expect = e-129
 Identities = 226/319 (70%), Positives = 264/319 (82%), Gaps = 1/319 (0%)
 Frame = +2

Query: 233  QWGSRPTQQQQLSQLLDFTKSLRGMKMATEINTLSDSDIYETPNSFTQIIHGDTLKESSG 412
            +WG++PTQQ+QL+QLLDF KSLRGMKMATEI+TL +  IYETP SF QII G   KES+G
Sbjct: 10   KWGTKPTQQEQLAQLLDFNKSLRGMKMATEIDTLENHKIYETPESFNQIIGG---KESAG 66

Query: 413  LYTSEATT-LEEACLNMMDLYCERARIQDGQTILDLGCGPGSLVFHVAKKFKNCKVTGVT 589
            L+T E TT +EEA   MMDLYCERA ++DG TILDLGCG G LV H+AKK+K  K+TG+T
Sbjct: 67   LFTDETTTTMEEANTKMMDLYCERAGLKDGHTILDLGCGAGLLVLHLAKKYKKSKITGIT 126

Query: 590  NSKPQKELMEEQSKKLNLSNVEIVLADVTSVEMESTFDRVLVIGLIEHMKNFELFLRKVS 769
            N+   KE + +Q K LNLSNVEI+LADVT V++ESTFDRV VIGLIEHMKNFELFLRK+S
Sbjct: 127  NTSSHKEYILKQCKNLNLSNVEIILADVTKVDIESTFDRVFVIGLIEHMKNFELFLRKIS 186

Query: 770  KWMKADGLLFLEHYCHKSFSYHCEPLSEDSWYDKNVFPRGTLLIPASTCLMYFQDDVTVQ 949
            KWMK DGLL LEH CHKSFS H EPLSED WY KN FP GTL+IP++TCL+YFQ+DVTV 
Sbjct: 187  KWMKDDGLLLLEHLCHKSFSDHWEPLSEDDWYAKNFFPSGTLVIPSATCLLYFQEDVTVI 246

Query: 950  DHWILSGNQYARSQNEWLKRIDGKVEEIREIFESFYGISKEEAVKLVNFWRIFCITGAEL 1129
            DHWILSGN +ARS    LKRIDGK+EE+++IF SFYGI +EEAVKL+N+WR+ CIT  EL
Sbjct: 247  DHWILSGNNFARSNEVILKRIDGKIEEVKDIFMSFYGIGREEAVKLINWWRLLCITANEL 306

Query: 1130 FGYNNGEEWSISHVLFKKK 1186
            F YNNGEEW IS +LFKKK
Sbjct: 307  FKYNNGEEWLISQLLFKKK 325


>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  409 bits (1052), Expect = e-112
 Identities = 188/346 (54%), Positives = 261/346 (75%)
 Frame = +2

Query: 149  DIWKNMELGLIPDXXXXXXXXXXXXXXXQWGSRPTQQQQLSQLLDFTKSLRGMKMATEIN 328
            ++ K +ELGL+P                QWG +PT ++Q++++ + T SLR MK+ATE+ 
Sbjct: 15   ELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNLTHSLRQMKIATEVE 74

Query: 329  TLSDSDIYETPNSFTQIIHGDTLKESSGLYTSEATTLEEACLNMMDLYCERARIQDGQTI 508
            TL DS +YE P  F +I++G  LK S   +  ++TTL+EA + M+DLYCERA+IQDGQ++
Sbjct: 75   TL-DSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSV 133

Query: 509  LDLGCGPGSLVFHVAKKFKNCKVTGVTNSKPQKELMEEQSKKLNLSNVEIVLADVTSVEM 688
            LDLGCG G+L  HVA+K+KNC+VT VTNS  QKE +EE+S++ NL NVE+ LAD+T+ EM
Sbjct: 134  LDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEM 193

Query: 689  ESTFDRVLVIGLIEHMKNFELFLRKVSKWMKADGLLFLEHYCHKSFSYHCEPLSEDSWYD 868
              T+DR+LVI L EHMKN+EL LRK+S+W+  DGLLFLEH CHK+F+YH EPL +D W+ 
Sbjct: 194  AETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFT 253

Query: 869  KNVFPRGTLLIPASTCLMYFQDDVTVQDHWILSGNQYARSQNEWLKRIDGKVEEIREIFE 1048
            + VFP GT++IP+++  +YFQDDV+V +HW LSG  ++R+  EWLKR+D  ++ I+ +FE
Sbjct: 254  EYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFE 313

Query: 1049 SFYGISKEEAVKLVNFWRIFCITGAELFGYNNGEEWSISHVLFKKK 1186
            +  G ++EEAVKL+N+WR FC++G E+FGYNNGEEW  SHVLFKKK
Sbjct: 314  TLMG-NEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 358


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
            gi|51980204|gb|AAU20766.1| (S)-coclaurine
            N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  402 bits (1034), Expect = e-110
 Identities = 182/346 (52%), Positives = 259/346 (74%)
 Frame = +2

Query: 149  DIWKNMELGLIPDXXXXXXXXXXXXXXXQWGSRPTQQQQLSQLLDFTKSLRGMKMATEIN 328
            ++ K +ELGL+PD               QWG + T ++Q++QL++ T SLR MK+ATE+ 
Sbjct: 17   ELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLVNLTHSLRQMKIATEVE 76

Query: 329  TLSDSDIYETPNSFTQIIHGDTLKESSGLYTSEATTLEEACLNMMDLYCERARIQDGQTI 508
            TL D  +YE P  F +I++G  LK S   + +++TTL+EA + M++LYCERA+I+DG ++
Sbjct: 77   TLDDQ-MYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAMLELYCERAQIKDGHSV 135

Query: 509  LDLGCGPGSLVFHVAKKFKNCKVTGVTNSKPQKELMEEQSKKLNLSNVEIVLADVTSVEM 688
            LDLGCG G+L  +VA+K+KN +VT VTNS  QKE +EE+S+K NLSNVE++LAD+T+ +M
Sbjct: 136  LDLGCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNLSNVEVLLADITTHKM 195

Query: 689  ESTFDRVLVIGLIEHMKNFELFLRKVSKWMKADGLLFLEHYCHKSFSYHCEPLSEDSWYD 868
              T+DR+LV+ L EHMKN+EL LRK+ +WM  DGLLF+EH CHK+F+YH EP+ ED W+ 
Sbjct: 196  PDTYDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAYHYEPIDEDDWFT 255

Query: 869  KNVFPRGTLLIPASTCLMYFQDDVTVQDHWILSGNQYARSQNEWLKRIDGKVEEIREIFE 1048
            + VFP GT++IP+++  +YFQDDV+V +HW LSG  ++R+  EWLKR+D  VE I+ +F 
Sbjct: 256  EYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANVELIKPMFV 315

Query: 1049 SFYGISKEEAVKLVNFWRIFCITGAELFGYNNGEEWSISHVLFKKK 1186
            +  G  ++EA+KL+N+WR FC++G E+FGYNNGEEW  SHVLFKKK
Sbjct: 316  TITGQCRQEAMKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 361


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
            gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
            [Thalictrum flavum]
          Length = 356

 Score =  395 bits (1015), Expect = e-107
 Identities = 186/350 (53%), Positives = 257/350 (73%)
 Frame = +2

Query: 137  DQVDDIWKNMELGLIPDXXXXXXXXXXXXXXXQWGSRPTQQQQLSQLLDFTKSLRGMKMA 316
            + V ++ K +E G + D               +WG +PT +QQL+QL+ F +SL+GM+MA
Sbjct: 9    EAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGMEMA 68

Query: 317  TEINTLSDSDIYETPNSFTQIIHGDTLKESSGLYTSEATTLEEACLNMMDLYCERARIQD 496
             E++TL D+++YE P  F  I+ G TLK S G +  E+TTL+E+ + MMDLYCERA+I+D
Sbjct: 69   EEVDTL-DAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERAQIKD 127

Query: 497  GQTILDLGCGPGSLVFHVAKKFKNCKVTGVTNSKPQKELMEEQSKKLNLSNVEIVLADVT 676
            GQ+ILDLGCG GSL  HVA+K++ CKVTG+TNS  QKE + +Q KKL+LSNVEI+L DVT
Sbjct: 128  GQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIILEDVT 187

Query: 677  SVEMESTFDRVLVIGLIEHMKNFELFLRKVSKWMKADGLLFLEHYCHKSFSYHCEPLSED 856
              E E T+DR+  + LIEHMKN+ELFL+KVS W+   GLLF+EH+CHK F+Y  EPL ED
Sbjct: 188  KFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQYEPLDED 247

Query: 857  SWYDKNVFPRGTLLIPASTCLMYFQDDVTVQDHWILSGNQYARSQNEWLKRIDGKVEEIR 1036
             WY + +FP GTL++ +S+ L+YFQ+DV+V +HW LSG   +    +WLKR+D  ++E++
Sbjct: 248  DWYTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVK 307

Query: 1037 EIFESFYGISKEEAVKLVNFWRIFCITGAELFGYNNGEEWSISHVLFKKK 1186
            EIFESFYG SKE+A+K + +WR+FCI  ++++  NNGEEW +S VLFKKK
Sbjct: 308  EIFESFYG-SKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQVLFKKK 356


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
            somniferum]
          Length = 351

 Score =  392 bits (1006), Expect = e-106
 Identities = 183/347 (52%), Positives = 249/347 (71%)
 Frame = +2

Query: 146  DDIWKNMELGLIPDXXXXXXXXXXXXXXXQWGSRPTQQQQLSQLLDFTKSLRGMKMATEI 325
            +++ +NMELGLIPD               QWG + T ++QLSQLLD   SL+GMKMATE+
Sbjct: 7    EELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEM 66

Query: 326  NTLSDSDIYETPNSFTQIIHGDTLKESSGLYTSEATTLEEACLNMMDLYCERARIQDGQT 505
              L D  +YE P  F +I HG  +K+S+G YT E+TTL+EA + M+DLY ERA+I+DGQ+
Sbjct: 67   ENL-DLKLYEAPMEFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQS 125

Query: 506  ILDLGCGPGSLVFHVAKKFKNCKVTGVTNSKPQKELMEEQSKKLNLSNVEIVLADVTSVE 685
            +LDLGCG G++    A KFK C+ TGVT+S  QK+ +E + K+L L+NV+++LAD+T+ E
Sbjct: 126  VLDLGCGLGAVALFGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITTYE 185

Query: 686  MESTFDRVLVIGLIEHMKNFELFLRKVSKWMKADGLLFLEHYCHKSFSYHCEPLSEDSWY 865
             E  FDR+  + LIEHMKN++L L+K+S+WMK DGLLF+EH CHK+ +YH EP+  + WY
Sbjct: 186  TEERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWY 245

Query: 866  DKNVFPRGTLLIPASTCLMYFQDDVTVQDHWILSGNQYARSQNEWLKRIDGKVEEIREIF 1045
               +FP GTL + +++ L+YFQDDV+V + W LSG  Y+RS  EWLK +D  + E +EI 
Sbjct: 246  TNYIFPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKEIM 305

Query: 1046 ESFYGISKEEAVKLVNFWRIFCITGAELFGYNNGEEWSISHVLFKKK 1186
             S     K EA+KL+NFWRIFC+ GAELFGY NGEEW ++H+LFKKK
Sbjct: 306  RSITKTEK-EAIKLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 351