BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver23_contig00009925
         (3663 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004136686.1| PREDICTED: probable ubiquitin conjugation fa...   714   0.0  
ref|XP_003633847.1| PREDICTED: probable ubiquitin conjugation fa...   694   0.0  
ref|XP_002532897.1| ubiquitin-protein ligase, putative [Ricinus ...   689   0.0  
gb|ABG89128.1| UFD2 [synthetic construct]                             687   0.0  
ref|NP_568313.2| putative ubiquitin conjugation factor E4 [Arabi...   685   0.0  

>ref|XP_004136686.1| PREDICTED: probable ubiquitin conjugation factor E4-like [Cucumis
            sativus] gi|449494681|ref|XP_004159617.1| PREDICTED:
            probable ubiquitin conjugation factor E4-like [Cucumis
            sativus]
          Length = 1043

 Score =  714 bits (1842), Expect = 0.0
 Identities = 441/1039 (42%), Positives = 607/1039 (58%), Gaps = 42/1039 (4%)
 Frame = +3

Query: 336  MVPGKRRRFICDEEDNIIRKILKVTLEKDSTLGEK------------SDGNFDKLKLCKD 479
            M   K +R   + ED I+RK+  ++L   S    +            S+G    L++ +D
Sbjct: 1    MATSKPQRSPEEVEDIILRKVFLISLTDTSDSDSRIVYLEQTAAELLSEGK--PLRISRD 58

Query: 480  LMVEVLKDRLSDGNSEAEQPLPYLIGCYRRANEEEEENQIKLAMVDINYTQCCAMNSISR 659
            +M  ++ DRLS     AE P  YLIGCYRRA++E +    K+A +  + T    M    +
Sbjct: 59   VMERIIIDRLSAHVPSAEPPFQYLIGCYRRAHDETK----KIASMK-DKTLRSDMEIALK 113

Query: 660  QAKELVVSHCLMHLVHSVRITNRNKDYDLTGLVRKHSETNFSESYLLPLLYSKV------ 821
            QAK+L +S+C +HL +    ++     DL         TN + S LLPL++S+V      
Sbjct: 114  QAKKLTISYCRIHLGNPELFSS---GADLG--------TNSNTSPLLPLIFSEVGGSSMD 162

Query: 822  ----SRKPCGALDCPHGFLEQFIEDSNFDSLNFVLIPLYEDLRTKVMEEDLLGDFEKPLK 989
                S    GA  CP GFLE+F+ DS+FD+L  +L  LYEDLR  V++   LG+F++PL+
Sbjct: 163  GFGASTSVGGAYQCPPGFLEEFLRDSDFDTLEPILKGLYEDLRGSVLKVSALGNFQQPLR 222

Query: 990  GLTYLVNNPICAKVLVNHPRWIPKVDMMNGITLESLSILGGFFNISLIPDDRFTMKKNWC 1169
             L +LV+ P+ AK LVNHP WIP     NG  +E  SILG FF++S +PD      +   
Sbjct: 223  ALRFLVSFPVGAKSLVNHPWWIPTGKYSNGRVIEMTSILGPFFHVSALPDHAIFKSQPDV 282

Query: 1170 PRQVIHCRLLTRRTNAESSFAKEVRTLTKKLHDGLEEILLSLLRNCETQENVFEYLSEII 1349
             +Q        R  +  SSF   ++T+   L+DGL E+LLSLL+N ET+ENV EYL+E+I
Sbjct: 283  GQQCFSEASTRRPADLLSSFTT-IKTVMNNLYDGLSEVLLSLLKNTETRENVLEYLAEVI 341

Query: 1350 RQNSSITKIQVDQ-NCGSFGMFVNLGAVMLRLCKTFIDKNLTKRSEIDGTYLLCNPHLDM 1526
             +NSS   IQVD  +C S GMFVNL A+MLRLC+ F+D NLTKR +ID  Y+  +  L++
Sbjct: 342  NRNSSRAHIQVDPLSCASSGMFVNLSAIMLRLCEPFLDANLTKRDKIDPKYVCYSNRLEL 401

Query: 1527 SGLTTLHASSKEVAAWISKLQK------GDGFDLQSNSSEEATXXXXXXXXXXXXXXDNS 1688
             GLT LHASS+EV  WI+   +      G   D +S   +                   +
Sbjct: 402  RGLTALHASSEEVTEWINNGTQLRTDNPGQSSDSESRLLQSQEASSSGSNATIGSSTAKA 461

Query: 1689 TSSCGKPRYTFGCEFFFMTARVLHLGLIKSLSELQRLHRKLVLNRNILPTMKALLGQASF 1868
             SS  K RY F CE FFMTARVL+LGL+K+ S+ + L + +    + L T+KA+ GQ   
Sbjct: 462  RSSSDKTRYPFICECFFMTARVLNLGLLKAFSDFKHLVQDISRCEDTLSTLKAMQGQGPA 521

Query: 1869 PQPEQDMEHLQSNVKSDWKNFCCYLSQILQDKALLKDALSFYHXXXXXXXXXXXXFKMPL 2048
            PQ E D+  L+  ++   +   CY +QIL+D  L++ AL+FY             FKMPL
Sbjct: 522  PQLEMDIARLEKEIELYSQEKLCYEAQILRDGTLIQQALTFYRLMVIWLVGLVGGFKMPL 581

Query: 2049 PSSCPIEFACVPEHLVADAIELLIAVFTYAESLADVIPEMDEYIKFVIMFMASPNYIRNS 2228
            PS+CP+EFA +PEH V DA+ELLI      ++L  +   +D+++ F+IMFMASP YIRN 
Sbjct: 582  PSACPMEFASMPEHFVEDAMELLIFASRIPKALDGI--NLDDFMNFIIMFMASPEYIRNP 639

Query: 2229 YIRQRMVEFLGLCIRNRSSSTSVITILEGSQLCLEFLVRNLLELYAGKEVIGSP----NK 2396
            Y+R +MVE L   I  RS S+   T+ EG QL LE+LVRNLL+LY   E  GS     +K
Sbjct: 640  YLRAKMVEVLNCWIPRRSGSSVTATLFEGHQLSLEYLVRNLLKLYVDIEFTGSHTQFYDK 699

Query: 2397 LQFRKSILEILGCLWEIPSHRDAWRQIA-EEDTAFYVVFLNVVINENIKLLDNDFHRI-- 2567
               R +I E+L  LW++PSHR+AWR IA EE+   Y+ FLN +IN++I LLD   ++I  
Sbjct: 700  FNIRHNIAELLEYLWQVPSHRNAWRMIAKEEEKGVYLNFLNFLINDSIYLLDESLNKILE 759

Query: 2568 LXXXXXXXXXXXXXXHVPSLWMQESALDFQFDKDMIGGCMSYITASVGMLAFTSEQIIVP 2747
            L                P+   QE    F   +++I   M      V MLAFTSEQI  P
Sbjct: 760  LKELEAEMSNTAEWERRPAQERQERTRLFHSQENIIRIDMKLANEDVSMLAFTSEQITAP 819

Query: 2748 FLLPHMVDTVVTMLNCFLSHFRSFHIKTSVPRRSMECGYCLEILLKQIVSIYVHLARGDS 2927
            FLLP MV+ V +MLN FL        K+   +   +  +    LLKQIV IYVHLARGD+
Sbjct: 820  FLLPEMVERVASMLNYFLLQLVGPQRKSLSLKDPEKYEFRPRELLKQIVQIYVHLARGDT 879

Query: 2928 ENIFAAAISKDSKSCIKQLF-----IDVGRVLQEDSLVLEFIALGTRVNDAALRAVQTEV 3092
            ENIF AAISKD +S  +QLF     + + R+ ++  ++ EF  LG +  DAA  A+  E 
Sbjct: 880  ENIFPAAISKDGRSYNEQLFTAAADVLIRRIREDSRIIQEFTDLGNKAKDAASEAMDAEA 939

Query: 3093 SLGDIPNDFLDPIEFNLMEDPVILPSMK-SVDRAVIQRHLLNYSTDPFNGLPLTQEMLIP 3269
            +LGDIP++FLDPI++ LM+DPVILPS + +VDR VIQRHLL+ STDPFN   LT +MLIP
Sbjct: 940  TLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDSTDPFNRSHLTADMLIP 999

Query: 3270 NVELKAKISEFITSKQCQE 3326
            N ELKA+I EFI S++ ++
Sbjct: 1000 NEELKARIKEFIRSQELKK 1018


>ref|XP_003633847.1| PREDICTED: probable ubiquitin conjugation factor E4-like [Vitis
            vinifera] gi|296082973|emb|CBI22274.3| unnamed protein
            product [Vitis vinifera]
          Length = 1037

 Score =  694 bits (1790), Expect = 0.0
 Identities = 443/1022 (43%), Positives = 603/1022 (59%), Gaps = 38/1022 (3%)
 Frame = +3

Query: 375  EDNIIRKILKVTL----EKDS-------TLGE-KSDGNFDKLKLCKDLMVEVLKDRLSDG 518
            ED I+ KI  V+L    E DS       T  E  S+G    LKL +DLM  VL DRLS  
Sbjct: 14   EDIILGKIFLVSLNDSMESDSRIVYLEMTAAEILSEGR--PLKLSRDLMERVLIDRLSGH 71

Query: 519  NSEAEQPLPYLIGCYRRANEEEEENQIKLAMVDINYTQCCAMNSISRQAKELVVSHCLMH 698
               AE P PYLIGCYRRA +E ++   K    D N      +  + +QAK+L VS+C +H
Sbjct: 72   FPGAEPPFPYLIGCYRRACDEGKKIASKK---DKNLRS--ELELVVKQAKKLAVSYCRIH 126

Query: 699  LVHSVRITNRNKDYDLTGLVRKHSETNFSESYLLPLLYSKVSRKPCG----ALDCPHGFL 866
            L +    +N +               + + S LLPL++S+VS    G    ++ CP GFL
Sbjct: 127  LGNPDMFSNWDS-----------GANDSAVSPLLPLIFSEVSSSVDGFGGSSIGCPPGFL 175

Query: 867  EQFIEDSNFDSLNFVLIPLYEDLRTKVMEEDLLGDFEKPLKGLTYLVNNPICAKVLVNHP 1046
            E+F  DS+FDSL+ +   LYE+LR+ V++   LG+F++PL+   YLV  P  AK LV+H 
Sbjct: 176  EEFFRDSDFDSLDPIFKGLYENLRSIVLKVSALGNFQQPLRAFLYLVRFPFGAKSLVSHR 235

Query: 1047 RWIPKVDMMNGITLESLSILGGFFNISLIPDDRFTMKKNWCPRQVIHCRLLTRRTNAESS 1226
             WIP+   MNG  +E  SILG FF++S +PD      +    +Q        R  +  SS
Sbjct: 236  WWIPQGAYMNGRVIEMTSILGPFFHVSALPDRGIFQGQPDVGQQCFSEASTRRPADLLSS 295

Query: 1227 FAKEVRTLTKKLHDGLEEILLSLLRNCETQENVFEYLSEIIRQNSSITKIQVDQ-NCGSF 1403
            F   ++T+   L+DGL E+LLSLL+N +T+E+V +YL+E+I +NSS   IQVD  +C S 
Sbjct: 296  FTT-IKTVMNGLYDGLAEVLLSLLKNADTRESVLKYLAEVINKNSSRAHIQVDPLSCASS 354

Query: 1404 GMFVNLGAVMLRLCKTFIDKNLTKRSEIDGTYLLCNPHLDMSGLTTLHASSKEVAAWISK 1583
            GMFV+L AVMLRLC+ F+D  LTK  +ID  Y+  +  LD+ GLT LHASS+EVA WI+K
Sbjct: 355  GMFVSLSAVMLRLCEPFLD--LTKMDKIDPKYVFYSTRLDLRGLTALHASSEEVAEWINK 412

Query: 1584 LQKG---------DGFDLQSNSSEEATXXXXXXXXXXXXXXDNSTSSCGKPRYTFGCEFF 1736
               G         DG      S E  +                   S  K +Y+F CE F
Sbjct: 413  DSPGGTEGSRQYSDGESRLLQSQEATSSGSNAHGPSFLHNAKPVPISSEKAKYSFICECF 472

Query: 1737 FMTARVLHLGLIKSLSELQRLHRKLVLNRNILPTMKALLGQASFPQPEQDMEHLQSNVKS 1916
            FMTARVL+LGL+K+ S+ + L + +    + L T+KA+ GQA  P+ E D+   +  ++ 
Sbjct: 473  FMTARVLNLGLLKAFSDFKHLVQDISRCEDSLATLKAVQGQAPSPELEADIARFEKEIEL 532

Query: 1917 DWKNFCCYLSQILQDKALLKDALSFYHXXXXXXXXXXXXFKMPLPSSCPIEFACVPEHLV 2096
              +   CY +QIL+D  LL+ ALSFY             FKMPLPS+CP+EFAC+PEH V
Sbjct: 533  YSQEKLCYEAQILRDGTLLQHALSFYRLMVVWLVRLIGGFKMPLPSTCPMEFACMPEHFV 592

Query: 2097 ADAIELLIAVFTYAESLADVIPEMDEYIKFVIMFMASPNYIRNSYIRQRMVEFLGLCIRN 2276
             DA+ELLI      ++L  V+  +D+++ F+IMFMASPN+IRN Y+R +MVE L   +  
Sbjct: 593  EDAMELLIFASRIPKALDGVL--LDDFMNFIIMFMASPNFIRNPYLRAKMVEVLNCWMPR 650

Query: 2277 RSSSTSVITILEGSQLCLEFLVRNLLELYAGKEVIGSP----NKLQFRKSILEILGCLWE 2444
            RS S++  T+ EG +L LE+LVRNLL+LY   E  GS     +K   R +I E+L  LW+
Sbjct: 651  RSGSSATTTLFEGHRLSLEYLVRNLLKLYVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQ 710

Query: 2445 IPSHRDAWRQIA-EEDTAFYVVFLNVVINENIKLLDNDFHRI--LXXXXXXXXXXXXXXH 2615
            +PSHR+AWRQIA EE+   Y+ FLN +IN++I LLD   ++I  L               
Sbjct: 711  VPSHRNAWRQIAKEEEKGVYLNFLNFLINDSIYLLDESLNKILELKELEAEMSNTVEWER 770

Query: 2616 VPSLWMQESALDFQFDKDMIGGCMSYITASVGMLAFTSEQIIVPFLLPHMVDTVVTMLNC 2795
             P+   QE    F   +++I   M      V MLAFTSEQI VPFLLP MV+ V  MLN 
Sbjct: 771  RPATERQERTRLFHSQENIIRIDMKLANEDVSMLAFTSEQITVPFLLPEMVERVANMLNY 830

Query: 2796 FLSHFRSFHIKTSVPRRSMECGYCLEILLKQIVSIYVHLARGDSENIFAAAISKDSKSCI 2975
            FL        K+   +   +  +  + LLKQIV IYVHLARGD++ IF  AISKD +S  
Sbjct: 831  FLLQLVGPQRKSLSLKDPEKYEFRPKQLLKQIVHIYVHLARGDTQKIFPTAISKDGRSYN 890

Query: 2976 KQLF---IDVGRVLQEDS-LVLEFIALGTRVNDAALRAVQTEVSLGDIPNDFLDPIEFNL 3143
            +QLF    DV R + ED  ++ EF  LG R   AA  A+  E +LG+IP++FLDPI++ L
Sbjct: 891  EQLFSAAADVLRRIGEDGRIIQEFSELGARAKVAASEAMDAEAALGEIPDEFLDPIQYTL 950

Query: 3144 MEDPVILPSMK-SVDRAVIQRHLLNYSTDPFNGLPLTQEMLIPNVELKAKISEFITSKQC 3320
            M+DPVILPS + +VDR VIQRHLL+ +TDPFN   LT +MLIPN+ELKA+I EFI S++ 
Sbjct: 951  MKDPVILPSSRITVDRPVIQRHLLSDNTDPFNRSHLTSDMLIPNIELKARIEEFIRSQEL 1010

Query: 3321 QE 3326
            ++
Sbjct: 1011 KK 1012


>ref|XP_002532897.1| ubiquitin-protein ligase, putative [Ricinus communis]
            gi|223527331|gb|EEF29477.1| ubiquitin-protein ligase,
            putative [Ricinus communis]
          Length = 1031

 Score =  689 bits (1777), Expect = 0.0
 Identities = 434/1023 (42%), Positives = 599/1023 (58%), Gaps = 33/1023 (3%)
 Frame = +3

Query: 348  KRRRFICDEEDNIIRKILKVTLEKDST-----------------LGEKSDGNFDKLKLCK 476
            K +R + + ED I+RKIL V+L  DS                  L E  D     LKL +
Sbjct: 6    KPQRSLQEIEDIILRKILLVSLTDDSISTTDSRIVYLEMAAAEILSEGKD-----LKLSR 60

Query: 477  DLMVEVLKDRLSDGNSEAEQPLPYLIGCYRRANEEEEENQIKLAMVDINYTQCCAMNSIS 656
            DL+  VL DRLS     +E P  YL+GCYRRA EEE +      M D N      +    
Sbjct: 61   DLIERVLIDRLSGQFPRSEPPFQYLLGCYRRATEEERKIS---NMKDKNVK--LELELSI 115

Query: 657  RQAKELVVSHCLMHLVHSVRITNRNKDYDLTGLVRKHSETNFSESYLLPLLYSKVSRKPC 836
            +QAK L +S+C +HL +       + D   + L           S LLPL+++ +     
Sbjct: 116  KQAKRLFISYCRIHLGNPDMFGGGDFDSKKSTL-----------SPLLPLIFASLGGFSI 164

Query: 837  --GALDCPHGFLEQFIEDSNFDSLNFVLIPLYEDLRTKVMEEDLLGDFEKPLKGLTYLVN 1010
              G+   P GFL++   D +FDSL+ +L  LYEDLR  V++   +G+F++PL  L +L+ 
Sbjct: 165  SGGSQPPPVGFLDEMFRDGDFDSLDPILKGLYEDLRGNVIKVSAMGNFQQPLGALLHLIT 224

Query: 1011 NPICAKVLVNHPRWIPKVDMMNGITLESLSILGGFFNISLIPDDRFTMKKNWCPRQVIHC 1190
             P+  K LVNHP WIPK   +NG  +E  SILG FF++S +PD      +    +Q    
Sbjct: 225  YPVGVKSLVNHPWWIPKGAYLNGRVIEMTSILGPFFHVSALPDHTIFKSEPDVGQQCFSE 284

Query: 1191 RLLTRRTNAESSFAKEVRTLTKKLHDGLEEILLSLLRNCETQENVFEYLSEIIRQNSSIT 1370
                R ++  SSFA  ++T    L+DGLE++L  LL+N +T+ENV +YL+E+I +NSS  
Sbjct: 285  VSTRRPSDLLSSFAT-IKTFMNNLYDGLEQVLRILLKNGDTRENVLQYLAEVINRNSSRA 343

Query: 1371 KIQVDQ-NCGSFGMFVNLGAVMLRLCKTFIDKNLTKRSEIDGTYLLCNPHLDMSGLTTLH 1547
             IQVD  +C S GMFVNL AVMLRLC  F+D NLTKR +ID  Y+  +  LD+ GLT LH
Sbjct: 344  HIQVDPLSCASSGMFVNLSAVMLRLCNPFLDPNLTKRDKIDSQYVFESNRLDLRGLTALH 403

Query: 1548 ASSKEVAAWISKLQKGDG-FDLQSNSSEEATXXXXXXXXXXXXXXDNSTSSCGKPRYTFG 1724
            ASS+EV  W++K   G     +QS+  E                   ++SS  K +YTF 
Sbjct: 404  ASSEEVTEWMNKGNHGKTEVSVQSSDGENRLLQSQEATSSGSGTNKPTSSSGQKAKYTFI 463

Query: 1725 CEFFFMTARVLHLGLIKSLSELQRLHRKLVLNRNILPTMKALLGQASFPQPEQDMEHLQS 1904
            CE FFMTARVL+LGL+K+ S+ + L + +    + L T+KA+  Q+  PQ + D+  L+ 
Sbjct: 464  CECFFMTARVLNLGLLKAFSDFKHLVQDISRCEDTLSTLKAMQEQSPTPQVQVDIARLEK 523

Query: 1905 NVKSDWKNFCCYLSQILQDKALLKDALSFYHXXXXXXXXXXXXFKMPLPSSCPIEFACVP 2084
            +++   +   CY +QIL+D+ L++ ALSFY             F+MPLP +CP+EFA +P
Sbjct: 524  DLELYSQEKFCYEAQILRDETLIQSALSFYRLMVVWLVDLVGGFRMPLPPTCPMEFASLP 583

Query: 2085 EHLVADAIELLIAVFTYAESLADVIPEMDEYIKFVIMFMASPNYIRNSYIRQRMVEFLGL 2264
            EH V DA+ELLI      ++L  V+  +D+++ F+IMFMASP YIRN Y+R +MVE L  
Sbjct: 584  EHFVEDAMELLIFASRIPKALDGVV--LDDFMNFIIMFMASPTYIRNPYLRAKMVEVLNC 641

Query: 2265 CIRNRSSSTSVITILEGSQLCLEFLVRNLLELYAGKEVIGSP----NKLQFRKSILEILG 2432
             +  RS S+   T+ EG  L LE+LVRNLL+LY   E  GS     +K   R +I E+L 
Sbjct: 642  WMPRRSGSSDTATLFEGHHLSLEYLVRNLLKLYVDIEFTGSHTQFYDKFNIRHNIAELLE 701

Query: 2433 CLWEIPSHRDAWRQIA-EEDTAFYVVFLNVVINENIKLLDNDFHRI--LXXXXXXXXXXX 2603
             LW++PSHR+AWRQIA EE+   Y+ FLN +IN++I LLD   ++I  L           
Sbjct: 702  YLWQVPSHRNAWRQIAREEEKGVYLNFLNFLINDSIYLLDESLNKILELKELEAEMSNTA 761

Query: 2604 XXXHVPSLWMQESALDFQFDKDMIGGCMSYITASVGMLAFTSEQIIVPFLLPHMVDTVVT 2783
                 P+   QE    F   +++I   M      V MLAFTSEQI  PFLL  MV+ V +
Sbjct: 762  EWEQRPAQERQERTRLFHSQENIIRIDMKLANEDVSMLAFTSEQITAPFLLLEMVERVAS 821

Query: 2784 MLNCFLSHFRSFHIKTSVPRRSMECGYCLEILLKQIVSIYVHLARGDSENIFAAAISKDS 2963
            MLN FL        K+   +   +  +  + LLKQIV IYVHL+RGD+ENIF AAISKD 
Sbjct: 822  MLNYFLLQLVGPQRKSLSLKDPEKYEFRPKQLLKQIVHIYVHLSRGDAENIFPAAISKDG 881

Query: 2964 KSCIKQLF---IDVGRVLQEDSLVL-EFIALGTRVNDAALRAVQTEVSLGDIPNDFLDPI 3131
            +S  +QLF    DV R + ED  V+ EF+ LG++   AA  A+ TE  LG+IP++FLDPI
Sbjct: 882  RSYNEQLFSAAADVLRRIGEDMRVIQEFVELGSKAKVAASEAMDTEAVLGEIPDEFLDPI 941

Query: 3132 EFNLMEDPVILPSMK-SVDRAVIQRHLLNYSTDPFNGLPLTQEMLIPNVELKAKISEFIT 3308
            ++ LM+DPVILPS + ++DR VIQRHLL+ +TDPFN   LT +MLIPNVELKA+I EFI 
Sbjct: 942  QYTLMKDPVILPSSRITIDRPVIQRHLLSDATDPFNRSHLTADMLIPNVELKARIEEFIR 1001

Query: 3309 SKQ 3317
            +++
Sbjct: 1002 NQE 1004


>gb|ABG89128.1| UFD2 [synthetic construct]
          Length = 1037

 Score =  687 bits (1772), Expect = 0.0
 Identities = 429/1033 (41%), Positives = 609/1033 (58%), Gaps = 36/1033 (3%)
 Frame = +3

Query: 336  MVPGKRRRFICDEEDNIIRKILKVTLEKDSTLGEK------------SDGNFDKLKLCKD 479
            M   K +R   + ED I+RKI  VTL + +    +            S+G   +L L +D
Sbjct: 1    MATSKPQRSPAEIEDIILRKIFYVTLTESTDSDPRIVYLEMTAAEILSEGK--ELLLSRD 58

Query: 480  LMVEVLKDRLSDGNSEAEQPLPYLIGCYRRANEEEEENQIKLAMVDINYTQCCAMNSISR 659
            LM  VL DRLS   S+AE P PYLIGC+RRA +E ++ Q   +M D N      M  +++
Sbjct: 59   LMERVLIDRLSGDFSDAEPPFPYLIGCHRRAYDESKKIQ---SMKDKNLRS--EMEIVTK 113

Query: 660  QAKELVVSHCLMHLVHSVRITNRNKDYDLTGLVRKHSETNFSESYLLPLLYSKVSRKPCG 839
            QAK+L VS+C +HL +     N +K     GL  +  + N S   +LPL++++V     G
Sbjct: 114  QAKKLAVSYCRIHLGNPDMFGNSDKPSG--GLDNRLKKRNVSP--VLPLIFAEVGS---G 166

Query: 840  ALD----------CPHGFLEQFIEDSNFDSLNFVLIPLYEDLRTKVMEEDLLGDFEKPLK 989
            +LD           P GFL++F +DS+FDSL+ +L  LYEDLR+ V+   +LGDF+ PL+
Sbjct: 167  SLDMFGASSSGVQAPPGFLDEFFKDSDFDSLDSILKELYEDLRSTVINVSVLGDFQPPLR 226

Query: 990  GLTYLVNNPICAKVLVNHPRWIPKVDMMNGITLESLSILGGFFNISLIPDDRFTMKKNWC 1169
             L YLV+ P+ AK LV+H  W+P+   MNG  +E  SILG FF+IS +PD+     +   
Sbjct: 227  ALKYLVSLPVGAKSLVSHEWWVPRGAYMNGRAMELTSILGPFFHISALPDNTLFKSQPDV 286

Query: 1170 PRQVIHCRLLTRRTNAESSFAKEVRTLTKKLHDGLEEILLSLLRNCETQENVFEYLSEII 1349
             +Q        R  +  SSF+  ++     L+ GL ++L+ LL++ +T+E V ++L+E+I
Sbjct: 287  GQQCFSEASERRPADLLSSFST-IKNFMNILYSGLHDVLMILLKSTDTRERVLQFLAEVI 345

Query: 1350 RQNSSITKIQVDQ-NCGSFGMFVNLGAVMLRLCKTFIDKNLTKRSEIDGTYLLCNPHLDM 1526
              N+S   IQVD  +C S GMFVNL AVMLRLC+ F+D +LTKR +ID  Y  C   L +
Sbjct: 346  NANASRAHIQVDPVSCASSGMFVNLSAVMLRLCEPFLDPHLTKRDKIDPKYAFCGHRLKL 405

Query: 1527 SGLTTLHASSKEVAAWISKLQKGDGFDL-QSNSSEEATXXXXXXXXXXXXXXDNSTSSCG 1703
            S LT LHASS+EV  WI K    +  D  + N +E                   +  S  
Sbjct: 406  SDLTALHASSEEVTEWIGKDAMANANDAGRENGNESRLLQSKEATSSSSNASGQNAKSAT 465

Query: 1704 KPRYTFGCEFFFMTARVLHLGLIKSLSELQRLHRKLVLNRNILPTMKALLGQASFPQPEQ 1883
            K  YTF CE FFMTARVL+LGL+K+LS+ + L + +    + L T+KA+  QA  PQ E 
Sbjct: 466  K--YTFICECFFMTARVLNLGLLKALSDFKHLAQDISRGEDNLATLKAMRDQAPSPQLEL 523

Query: 1884 DMEHLQSNVKSDWKNFCCYLSQILQDKALLKDALSFYHXXXXXXXXXXXXFKMPLPSSCP 2063
            D+  ++  ++   +   C+ +QIL+D   ++ ALSFY             FKMPLPS+CP
Sbjct: 524  DISRMEKELELSSQEKLCHEAQILRDGDFIQRALSFYRLMVVWLVGLVGGFKMPLPSTCP 583

Query: 2064 IEFACVPEHLVADAIELLIAVFTYAESLADVIPEMDEYIKFVIMFMASPNYIRNSYIRQR 2243
            +EF+C+PEH V DA+ELLI      ++L D +P +D+++ F+IMFMASP Y+RN Y+R +
Sbjct: 584  MEFSCMPEHFVEDAMELLIFASRIPKAL-DGVP-LDDFMNFIIMFMASPEYVRNPYLRAK 641

Query: 2244 MVEFLGLCIRNRSSSTSVITILEGSQLCLEFLVRNLLELYAGKEVIGSP----NKLQFRK 2411
            MVE L   +   SSS++  T+ EG QL LE+LVRNLL+LY   E  GS     +K   R 
Sbjct: 642  MVEVLNCWMPRSSSSSATSTLFEGHQLSLEYLVRNLLKLYVDIEFTGSHTQFYDKFNIRH 701

Query: 2412 SILEILGCLWEIPSHRDAWRQIA-EEDTAFYVVFLNVVINENIKLLDNDFHRIL--XXXX 2582
            +I E+L  LW++PSHR+AWR+IA +E+   Y+ FLN ++N++I LLD   ++IL      
Sbjct: 702  NIAELLEYLWQVPSHRNAWRRIAKDEEKGVYLNFLNFLVNDSIYLLDESLNKILEIKQIE 761

Query: 2583 XXXXXXXXXXHVPSLWMQESALDFQFDKDMIGGCMSYITASVGMLAFTSEQIIVPFLLPH 2762
                        P+   QE    F   ++++   M      V MLAFTSE+I  PFLLP 
Sbjct: 762  ADMSNTAEWEQRPTQERQERTRLFHSQENIVRIDMKLANEDVTMLAFTSEEITAPFLLPE 821

Query: 2763 MVDTVVTMLNCFLSHFRSFHIKTSVPRRSMECGYCLEILLKQIVSIYVHLARGDSENIFA 2942
            MV+ V  MLN FL        K+   +   +  +  + LLKQIV IYV+LARGD+ NIF 
Sbjct: 822  MVERVANMLNYFLLQLVGPQRKSLSLKDPEKYEFRPKQLLKQIVRIYVNLARGDTVNIFP 881

Query: 2943 AAISKDSKSCIKQLF---IDV-GRVLQEDSLVLEFIALGTRVNDAALRAVQTEVSLGDIP 3110
             AIS D +S  +QLF    DV  R+ +E  ++ EF+ LGT+   AA  A+  E +LG+IP
Sbjct: 882  GAISSDGRSYNEQLFNAGADVLRRIGEEGRIIQEFMELGTKAKAAASEALDAEAALGEIP 941

Query: 3111 NDFLDPIEFNLMEDPVILPSMK-SVDRAVIQRHLLNYSTDPFNGLPLTQEMLIPNVELKA 3287
            ++FLDPI++ LM DPVILPS + +VDR +IQRHLL+ + DPFN   LT +MLIP++ELKA
Sbjct: 942  DEFLDPIQYTLMRDPVILPSSRITVDRPIIQRHLLSDNHDPFNRAHLTSDMLIPDIELKA 1001

Query: 3288 KISEFITSKQCQE 3326
            KI EF+ S Q ++
Sbjct: 1002 KIDEFVKSHQSKK 1014


>ref|NP_568313.2| putative ubiquitin conjugation factor E4 [Arabidopsis thaliana]
            gi|75174048|sp|Q9LF41.1|UBE4_ARATH RecName: Full=Probable
            ubiquitin conjugation factor E4; AltName: Full=Plant
            U-box protein 1; AltName: Full=U-box domain-containing
            protein 1; AltName: Full=Ubiquitin-fusion degradation
            protein 2-like; Short=UB fusion protein 2-like
            gi|9755795|emb|CAC01739.1| ubiquitin-fusion degradation
            protein-like [Arabidopsis thaliana]
            gi|332004773|gb|AED92156.1| putative ubiquitin
            conjugation factor E4 [Arabidopsis thaliana]
          Length = 1038

 Score =  685 bits (1768), Expect = 0.0
 Identities = 430/1034 (41%), Positives = 611/1034 (59%), Gaps = 37/1034 (3%)
 Frame = +3

Query: 336  MVPGKRRRFICDEEDNIIRKILKVTLEKDSTLGEK------------SDGNFDKLKLCKD 479
            M   K +R   + ED I+RKI  VTL + +    +            S+G   +L L +D
Sbjct: 1    MATSKPQRSPAEIEDIILRKIFYVTLTESTDSDPRIVYLEMTAAEILSEGK--ELLLSRD 58

Query: 480  LMVEVLKDRLSDGNSEAEQPLPYLIGCYRRANEEEEENQIKLAMVDINYTQCCAMNSISR 659
            LM  VL DRLS   S+AE P PYLIGC+RRA +E ++ Q   +M D N      M  +++
Sbjct: 59   LMERVLIDRLSGDFSDAEPPFPYLIGCHRRAYDESKKIQ---SMKDKNLRS--EMEIVTK 113

Query: 660  QAKELVVSHCLMHLVHSVRITNRNKDYDLTGLVRKHSETNFSESYLLPLLYSKVSRKPCG 839
            QAK+L VS+C +HL +     N +K     GL  +  + N S   +LPL++++V     G
Sbjct: 114  QAKKLAVSYCRIHLGNPDMFGNSDKPSG--GLDNRLKKRNVSP--VLPLIFAEVGS---G 166

Query: 840  ALD----------CPHGFLEQFIEDSNFDSLNFVLIPLYEDLRTKVMEEDLLGDFEKPLK 989
            +LD           P GFL++F +DS+FDSL+ +L  LYEDLR+ V+   +LGDF+ PL+
Sbjct: 167  SLDMFGASSSGVQAPPGFLDEFFKDSDFDSLDSILKELYEDLRSTVINVSVLGDFQPPLR 226

Query: 990  GLTYLVNNPICAKVLVNHPRWIPKVDMMNGITLESLSILGGFFNISLIPDDRFTMKKNWC 1169
             L YLV+ P+ AK LV+H  W+P+   MNG  +E  SILG FF+IS +PD+     +   
Sbjct: 227  ALKYLVSLPVGAKSLVSHEWWVPRGAYMNGRAMELTSILGPFFHISALPDNTLFKSQPDV 286

Query: 1170 PRQVIHCRLLTRRTNAESSFAKEVRTLTKKLHDGLEEILLSLLRNCETQENVFEYLSEII 1349
             +Q        R  +  SSF+  ++     L+ GL ++L+ LL++ +T+E V ++L+E+I
Sbjct: 287  GQQCFSEASERRPADLLSSFST-IKNFMNILYSGLHDVLMILLKSTDTRERVLQFLAEVI 345

Query: 1350 RQNSSITKIQVDQ-NCGSFGMFVNLGAVMLRLCKTFIDKNLTKRSEIDGTYLLCNPHLDM 1526
              N+S   IQVD  +C S GMFVNL AVMLRLC+ F+D +LTKR +ID  Y  C   L +
Sbjct: 346  NANASRAHIQVDPVSCASSGMFVNLSAVMLRLCEPFLDPHLTKRDKIDPKYAFCGHRLKL 405

Query: 1527 SGLTTLHASSKEVAAWISKLQKGDGFDL-QSNSSEEATXXXXXXXXXXXXXXDNSTSSCG 1703
            S LT LHASS+EV  WI K    +  D  + N +E                   +  S  
Sbjct: 406  SDLTALHASSEEVTEWIGKDAMANANDAGRENGNESRLLQSKEATSSSSNASGQNAKSAT 465

Query: 1704 KPRYTFGCEFFFMTARVLHLGLIKSLSELQRLHRKLVLNRNILPTMKALLGQASFPQPEQ 1883
            K  YTF CE FFMTARVL+LGL+K+LS+ + L + +    + L T+KA+  QA  PQ E 
Sbjct: 466  K--YTFICECFFMTARVLNLGLLKALSDFKHLAQDISRGEDNLATLKAMRDQAPSPQLEL 523

Query: 1884 DMEHLQSNVKSDWKNFCCYLSQILQDKALLKDALSFYHXXXXXXXXXXXXFKMPLPSSCP 2063
            D+  ++  ++   +   C+ +QIL+D   ++ ALSFY             FKMPLPS+CP
Sbjct: 524  DISRMEKELELSSQEKLCHEAQILRDGDFIQRALSFYRLMVVWLVGLVGGFKMPLPSTCP 583

Query: 2064 IEFACVPEHLVADAIELLIAVFTYAESLADVIPEMDEYIKFVIMFMASPNYIRNSYIRQR 2243
            +EF+C+PEH V DA+ELLI      ++L D +P +D+++ F+IMFMASP Y+RN Y+R +
Sbjct: 584  MEFSCMPEHFVEDAMELLIFASRIPKAL-DGVP-LDDFMNFIIMFMASPEYVRNPYLRAK 641

Query: 2244 MVEFLGLCI-RNRSSSTSVITILEGSQLCLEFLVRNLLELYAGKEVIGSP----NKLQFR 2408
            MVE L   + R+ SSS++  T+ EG QL LE+LVRNLL+LY   E  GS     +K   R
Sbjct: 642  MVEVLNCWMPRSSSSSSATSTLFEGHQLSLEYLVRNLLKLYVDIEFTGSHTQFYDKFNIR 701

Query: 2409 KSILEILGCLWEIPSHRDAWRQIA-EEDTAFYVVFLNVVINENIKLLDNDFHRIL--XXX 2579
             +I E+L  LW++PSHR+AWR+IA +E+   Y+ FLN ++N++I LLD   ++IL     
Sbjct: 702  HNIAELLEYLWQVPSHRNAWRRIAKDEEKGVYLNFLNFLVNDSIYLLDESLNKILEIKQI 761

Query: 2580 XXXXXXXXXXXHVPSLWMQESALDFQFDKDMIGGCMSYITASVGMLAFTSEQIIVPFLLP 2759
                         P+   QE    F   ++++   M      V MLAFTSE+I  PFLLP
Sbjct: 762  EADMSNTAEWEQRPTQERQERTRLFHSQENIVRIDMKLANEDVTMLAFTSEEITAPFLLP 821

Query: 2760 HMVDTVVTMLNCFLSHFRSFHIKTSVPRRSMECGYCLEILLKQIVSIYVHLARGDSENIF 2939
             MV+ V  MLN FL        K+   +   +  +  + LLKQIV IYV+LARGD+ NIF
Sbjct: 822  EMVERVANMLNYFLLQLVGPQRKSLSLKDPEKYEFRPKQLLKQIVRIYVNLARGDTVNIF 881

Query: 2940 AAAISKDSKSCIKQLF---IDV-GRVLQEDSLVLEFIALGTRVNDAALRAVQTEVSLGDI 3107
              AIS D +S  +QLF    DV  R+ +E  ++ EF+ LGT+   AA  A+  E +LG+I
Sbjct: 882  PGAISSDGRSYNEQLFNAGADVLRRIGEEGRIIQEFMELGTKAKAAASEALDAEAALGEI 941

Query: 3108 PNDFLDPIEFNLMEDPVILPSMK-SVDRAVIQRHLLNYSTDPFNGLPLTQEMLIPNVELK 3284
            P++FLDPI++ LM DPVILPS + +VDR +IQRHLL+ + DPFN   LT +MLIP++ELK
Sbjct: 942  PDEFLDPIQYTLMRDPVILPSSRITVDRPIIQRHLLSDNHDPFNRAHLTSDMLIPDIELK 1001

Query: 3285 AKISEFITSKQCQE 3326
            AKI EF+ S Q ++
Sbjct: 1002 AKIDEFVKSHQSKK 1015