BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver23_contig00009834
         (1910 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002312838.1| l-ascorbate oxidase precursor [Populus trich...   742   0.0  
gb|AAF35911.2|AF233594_1 ascorbate oxidase AO4 [Cucumis melo]         725   0.0  
sp|P24792.2|ASO_CUCMA RecName: Full=L-ascorbate oxidase; Short=A...   725   0.0  
sp|P37064.1|ASO_CUCPM RecName: Full=L-ascorbate oxidase; Short=A...   723   0.0  
gb|ABK96191.1| unknown [Populus trichocarpa]                          723   0.0  

>ref|XP_002312838.1| l-ascorbate oxidase precursor [Populus trichocarpa]
            gi|222849246|gb|EEE86793.1| l-ascorbate oxidase precursor
            [Populus trichocarpa]
          Length = 597

 Score =  742 bits (1915), Expect = 0.0
 Identities = 353/543 (65%), Positives = 420/543 (77%), Gaps = 2/543 (0%)
 Frame = +3

Query: 3    INGQFPGPEIRAKAGDTIVVNLSNKLNTKEGLVIHWHGIRQLDTPWADGTASVSQCAINP 182
            ING+FPGP IRA+AGDT+ V+L+NKL+T EG+VIHWHGIRQ  TPWADGTAS+SQCAINP
Sbjct: 68   INGKFPGPTIRARAGDTVHVHLTNKLHT-EGVVIHWHGIRQKGTPWADGTASISQCAINP 126

Query: 183  GATFQYRFIVDRAGTYFYHGHYEMQRAAGLYGSLIVDVADGKIEPFPYHGEINLLLSDWW 362
            G +F YRF VDRAGTYFYHGHY MQR+AGLYGSLIVDVA+G+ EPF Y GE +LLLSDWW
Sbjct: 127  GESFDYRFTVDRAGTYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDGEFDLLLSDWW 186

Query: 363  HQSAHDQEVALSSNPFRWIGEPQSILINGRGQYNCSMSAKYDNTTNF--CNIYGRPSDQC 536
            H+SAH QEV LSS P RWIGEPQ++L+NGRGQY CS++A Y N ++   CN+ G   +Q 
Sbjct: 187  HESAHHQEVGLSSRPMRWIGEPQTLLVNGRGQYGCSLAAHYSNNSSLSQCNVTGH--EQW 244

Query: 537  EPYVLSVEPRKFYRLRIASTTSLASLNLVIANHRMLLVEADGNYLQLKYVTNLDIYSGES 716
             PY+L V+P K YR+R++STT+LASLNL I NH+ML+VEADGNYLQ     +LDIYSGES
Sbjct: 245  APYILHVDPNKTYRIRLSSTTALASLNLAIGNHKMLVVEADGNYLQPFETDDLDIYSGES 304

Query: 717  YSVILNTGQDPSVNYWVSVSQRGRKPPLPPPALTILHYKPNSVFGIKPSSPPPLSPAWDD 896
            YSV+L T QDPS NYW+S   RGRKP   P ALTIL+YK NS     P SPPP++P WDD
Sbjct: 305  YSVLLKTSQDPSQNYWISFGVRGRKPQ-TPQALTILNYKTNSASKF-PLSPPPVTPRWDD 362

Query: 897  LAQSKNFTNKVLGLQVDGISLQPHPSAIPRPPVRHDRRIFLLNTQTKIDGYVKWSVNNIS 1076
             A SK FTNKV  L             IP+PP  + RRI LLNTQ K++GY KWS+NN+S
Sbjct: 363  YAHSKAFTNKVKALD---------HKTIPKPPSTYHRRIILLNTQNKMNGYTKWSINNVS 413

Query: 1077 LVLPTTPYLGSLKFGLKNGFNATTPPETFPSTYDITKPPANPNSMYGSGVYHFKHMEIVD 1256
            L LP TPYLGS++FGL+NGF+ T PPE+FP  YD+ KPP NPN+  G+GVY   +   VD
Sbjct: 414  LSLPATPYLGSIRFGLQNGFDQTKPPESFPVQYDVMKPPGNPNTTTGNGVYMLSYYSTVD 473

Query: 1257 VILQNANVLGEGVSEIHPWHLHGHDFWVLGYGDGKFSEENDEKNLNLVDPPLRNTVVVFP 1436
            VILQNAN L E VSEIHPWHLHGHDFWVLGYG+GKF+++ DEK  N+ +PP RN+ V+FP
Sbjct: 474  VILQNANALAENVSEIHPWHLHGHDFWVLGYGEGKFTKD-DEKKFNMKNPPYRNSAVIFP 532

Query: 1437 YGWTAIRFIADNPGVWPFHCHIEPHLHMGMGVIFAVAVDKIKQIPDGVLDCGLTATTSPT 1616
            YGWTA+RF+ADNPGVW FHCHIEPHLHMGMGV+ A  V ++ +IP   L CGLT     T
Sbjct: 533  YGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVLAEGVQRLPKIPKEALSCGLTGKKFMT 592

Query: 1617 ISN 1625
             +N
Sbjct: 593  GNN 595


>gb|AAF35911.2|AF233594_1 ascorbate oxidase AO4 [Cucumis melo]
          Length = 587

 Score =  725 bits (1872), Expect = 0.0
 Identities = 343/533 (64%), Positives = 406/533 (76%)
 Frame = +3

Query: 3    INGQFPGPEIRAKAGDTIVVNLSNKLNTKEGLVIHWHGIRQLDTPWADGTASVSQCAINP 182
            INGQFPGP IRA AGD +VV L+NKL+T EG+VIHWHGI Q  TPWADGTAS+SQCAINP
Sbjct: 62   INGQFPGPTIRANAGDMVVVELTNKLHT-EGVVIHWHGILQRGTPWADGTASISQCAINP 120

Query: 183  GATFQYRFIVDRAGTYFYHGHYEMQRAAGLYGSLIVDVADGKIEPFPYHGEINLLLSDWW 362
            G TF YRF+VD+AGTYFYHGH  MQR+AGLYGSLIVD  +G  EPF Y  EINLLLSDWW
Sbjct: 121  GETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWW 180

Query: 363  HQSAHDQEVALSSNPFRWIGEPQSILINGRGQYNCSMSAKYDNTTNFCNIYGRPSDQCEP 542
            HQS H QEV LSS P RWIGEPQSILINGRGQ++CS++AKY N    C + G+  +QC P
Sbjct: 181  HQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGK--EQCAP 238

Query: 543  YVLSVEPRKFYRLRIASTTSLASLNLVIANHRMLLVEADGNYLQLKYVTNLDIYSGESYS 722
            ++L V P+K YR+RIASTT+LASLN  I NH++L+VEADGNY+Q    +++DIYSGESYS
Sbjct: 239  FILHVHPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYS 298

Query: 723  VILNTGQDPSVNYWVSVSQRGRKPPLPPPALTILHYKPNSVFGIKPSSPPPLSPAWDDLA 902
            V++ T Q+PS NYWVS+  R R P  PP  LT+L+Y PNSV  + P SPPP +P W+D  
Sbjct: 299  VLITTDQNPSENYWVSIGVRARLPKTPP-GLTVLNYLPNSVSKL-PISPPPETPDWEDFD 356

Query: 903  QSKNFTNKVLGLQVDGISLQPHPSAIPRPPVRHDRRIFLLNTQTKIDGYVKWSVNNISLV 1082
            +SKNFT ++                 P+PPVR++RR+FLLNTQ +I+G++KW++NN+SL 
Sbjct: 357  RSKNFTFRIFAAM-----------GSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLA 405

Query: 1083 LPTTPYLGSLKFGLKNGFNATTPPETFPSTYDITKPPANPNSMYGSGVYHFKHMEIVDVI 1262
            LP TPYL ++KF L   FN   PPETFP  YDI  PP NP +  G+GVY F   E VDVI
Sbjct: 406  LPPTPYLAAMKFRLSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVI 465

Query: 1263 LQNANVLGEGVSEIHPWHLHGHDFWVLGYGDGKFSEENDEKNLNLVDPPLRNTVVVFPYG 1442
            LQNAN+L    SEIHPWHLHGHDFWVLGYG+GKFS   D + LNL +PPLRNTVV+FPYG
Sbjct: 466  LQNANMLNANTSEIHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYG 525

Query: 1443 WTAIRFIADNPGVWPFHCHIEPHLHMGMGVIFAVAVDKIKQIPDGVLDCGLTA 1601
            WTAIRF+ADNPGVW FHCHIEPHLHMGMGVIFA  V K+  IP   + CG TA
Sbjct: 526  WTAIRFVADNPGVWAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA 578


>sp|P24792.2|ASO_CUCMA RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase; Flags:
            Precursor gi|885589|dbj|BAA09528.1| ascorbate oxidase
            [Cucurbita maxima]
          Length = 579

 Score =  725 bits (1871), Expect = 0.0
 Identities = 347/533 (65%), Positives = 411/533 (77%)
 Frame = +3

Query: 3    INGQFPGPEIRAKAGDTIVVNLSNKLNTKEGLVIHWHGIRQLDTPWADGTASVSQCAINP 182
            INGQFPGP IRA AGDT+VV L NKL+T EG+VIHWHGI Q  TPWADGTAS+SQCAINP
Sbjct: 57   INGQFPGPTIRANAGDTVVVELINKLHT-EGVVIHWHGILQRGTPWADGTASISQCAINP 115

Query: 183  GATFQYRFIVDRAGTYFYHGHYEMQRAAGLYGSLIVDVADGKIEPFPYHGEINLLLSDWW 362
            G TF Y F VD  GT+FYHGH  MQR+AGLYGSLIVD   GK EPF Y GEINLLLSDWW
Sbjct: 116  GETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWW 175

Query: 363  HQSAHDQEVALSSNPFRWIGEPQSILINGRGQYNCSMSAKYDNTTNFCNIYGRPSDQCEP 542
            HQS H QEV LSS P RWIGEPQ+IL+NGRGQ++CS++AKYD+    C + G  S+ C P
Sbjct: 176  HQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKG--SEPCAP 233

Query: 543  YVLSVEPRKFYRLRIASTTSLASLNLVIANHRMLLVEADGNYLQLKYVTNLDIYSGESYS 722
            Y+  V P+K YR+RIASTT+LA+LN  I NH +L+VEADGNY+Q  Y +++DIYSGESYS
Sbjct: 234  YIFHVMPKKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYS 293

Query: 723  VILNTGQDPSVNYWVSVSQRGRKPPLPPPALTILHYKPNSVFGIKPSSPPPLSPAWDDLA 902
            V++ T Q+PS NYWVSV  RGR P   PP LT+L+Y PNSV  + P+SPPP +PAWDD  
Sbjct: 294  VLITTDQNPSENYWVSVGTRGRHPN-TPPGLTLLNYLPNSVSKL-PTSPPPETPAWDDFD 351

Query: 903  QSKNFTNKVLGLQVDGISLQPHPSAIPRPPVRHDRRIFLLNTQTKIDGYVKWSVNNISLV 1082
            +SKNFT ++                 P+PPV+ +RRIFLLNTQ  I+GYVKW++N++SL 
Sbjct: 352  RSKNFTYRITAAM-----------GSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLA 400

Query: 1083 LPTTPYLGSLKFGLKNGFNATTPPETFPSTYDITKPPANPNSMYGSGVYHFKHMEIVDVI 1262
            LP TPYLG++KF L + F+   PPE FP  YDI  PP N  +  G+GVY FK  EIVDVI
Sbjct: 401  LPPTPYLGAMKFNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVI 460

Query: 1263 LQNANVLGEGVSEIHPWHLHGHDFWVLGYGDGKFSEENDEKNLNLVDPPLRNTVVVFPYG 1442
            LQNAN++ E +SEIHPWHLHGHDFWVLGYGDGKF+ E +E +LNL +PPLRNTVV+FPYG
Sbjct: 461  LQNANMMKENLSEIHPWHLHGHDFWVLGYGDGKFTAE-EESSLNLKNPPLRNTVVIFPYG 519

Query: 1443 WTAIRFIADNPGVWPFHCHIEPHLHMGMGVIFAVAVDKIKQIPDGVLDCGLTA 1601
            WTAIRF+ADNPGVW FHCHIEPHLHMGMGV+FA  V+K+ +IP   L CG TA
Sbjct: 520  WTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTA 572


>sp|P37064.1|ASO_CUCPM RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase
            gi|442635|pdb|1AOZ|A Chain A, Refined Crystal Structure
            Of Ascorbate Oxidase At 1.9 Angstroms Resolution
            gi|442636|pdb|1AOZ|B Chain B, Refined Crystal Structure
            Of Ascorbate Oxidase At 1.9 Angstroms Resolution
            gi|493837|pdb|1ASO|A Chain A, X-Ray Structures And
            Mechanistic Implications Of Three Functional Derivatives
            Of Ascorbate Oxidase From Zucchini: Reduced-, Peroxide-,
            And Azide-Forms gi|493838|pdb|1ASO|B Chain B, X-Ray
            Structures And Mechanistic Implications Of Three
            Functional Derivatives Of Ascorbate Oxidase From
            Zucchini: Reduced-, Peroxide-, And Azide-Forms
            gi|493839|pdb|1ASP|A Chain A, X-ray Structures And
            Mechanistic Implications Of Three Functional Derivatives
            Of Ascorbate Oxidase From Zucchini: Reduced-, Peroxide-,
            And Azide-forms gi|493840|pdb|1ASP|B Chain B, X-ray
            Structures And Mechanistic Implications Of Three
            Functional Derivatives Of Ascorbate Oxidase From
            Zucchini: Reduced-, Peroxide-, And Azide-forms
            gi|493841|pdb|1ASQ|A Chain A, X-Ray Structures And
            Mechanistic Implications Of Three Functional Derivatives
            Of Ascorbate Oxidase From Zucchini: Reduced-, Peroxide-,
            And Azide-Forms gi|493842|pdb|1ASQ|B Chain B, X-Ray
            Structures And Mechanistic Implications Of Three
            Functional Derivatives Of Ascorbate Oxidase From
            Zucchini: Reduced-, Peroxide-, And Azide-Forms
          Length = 552

 Score =  723 bits (1867), Expect = 0.0
 Identities = 346/546 (63%), Positives = 416/546 (76%)
 Frame = +3

Query: 3    INGQFPGPEIRAKAGDTIVVNLSNKLNTKEGLVIHWHGIRQLDTPWADGTASVSQCAINP 182
            INGQFPGP IRA AGD++VV L+NKL+T EG+VIHWHGI Q  TPWADGTAS+SQCAINP
Sbjct: 27   INGQFPGPTIRANAGDSVVVELTNKLHT-EGVVIHWHGILQRGTPWADGTASISQCAINP 85

Query: 183  GATFQYRFIVDRAGTYFYHGHYEMQRAAGLYGSLIVDVADGKIEPFPYHGEINLLLSDWW 362
            G TF Y F VD  GT+FYHGH  MQR+AGLYGSLIVD   GK EPF Y GEINLLLSDWW
Sbjct: 86   GETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWW 145

Query: 363  HQSAHDQEVALSSNPFRWIGEPQSILINGRGQYNCSMSAKYDNTTNFCNIYGRPSDQCEP 542
            HQS H QEV LSS P RWIGEPQ+IL+NGRGQ++CS++AKYD+    C + G  S+ C P
Sbjct: 146  HQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKG--SESCAP 203

Query: 543  YVLSVEPRKFYRLRIASTTSLASLNLVIANHRMLLVEADGNYLQLKYVTNLDIYSGESYS 722
            Y+  V P+K YR+RIASTT+LA+LN  I NH++L+VEADGNY+Q  Y +++DIYSGESYS
Sbjct: 204  YIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYS 263

Query: 723  VILNTGQDPSVNYWVSVSQRGRKPPLPPPALTILHYKPNSVFGIKPSSPPPLSPAWDDLA 902
            V++ T Q+PS NYWVSV  R R P   PP LT+L+Y PNSV  + P+SPPP +PAWDD  
Sbjct: 264  VLITTDQNPSENYWVSVGTRARHPN-TPPGLTLLNYLPNSVSKL-PTSPPPQTPAWDDFD 321

Query: 903  QSKNFTNKVLGLQVDGISLQPHPSAIPRPPVRHDRRIFLLNTQTKIDGYVKWSVNNISLV 1082
            +SKNFT ++                 P+PPV+ +RRIFLLNTQ  I+GYVKW++N++SL 
Sbjct: 322  RSKNFTYRITAAM-----------GSPKPPVKFNRRIFLLNTQNVINGYVKWAINDVSLA 370

Query: 1083 LPTTPYLGSLKFGLKNGFNATTPPETFPSTYDITKPPANPNSMYGSGVYHFKHMEIVDVI 1262
            LP TPYLG++K+ L + F+   PPE FP  YDI  PP N  +  G+GVY FK  E+VDVI
Sbjct: 371  LPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVI 430

Query: 1263 LQNANVLGEGVSEIHPWHLHGHDFWVLGYGDGKFSEENDEKNLNLVDPPLRNTVVVFPYG 1442
            LQNAN++ E +SE HPWHLHGHDFWVLGYGDGKFS E +E +LNL +PPLRNTVV+FPYG
Sbjct: 431  LQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAE-EESSLNLKNPPLRNTVVIFPYG 489

Query: 1443 WTAIRFIADNPGVWPFHCHIEPHLHMGMGVIFAVAVDKIKQIPDGVLDCGLTATTSPTIS 1622
            WTAIRF+ADNPGVW FHCHIEPHLHMGMGV+FA  V+K+ +IP   L CG TA    ++ 
Sbjct: 490  WTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTA---KSLI 546

Query: 1623 NNAVGP 1640
            NN   P
Sbjct: 547  NNPKNP 552


>gb|ABK96191.1| unknown [Populus trichocarpa]
          Length = 594

 Score =  723 bits (1865), Expect = 0.0
 Identities = 347/533 (65%), Positives = 412/533 (77%), Gaps = 1/533 (0%)
 Frame = +3

Query: 3    INGQFPGPEIRAKAGDTIVVNLSNKLNTKEGLVIHWHGIRQLDTPWADGTASVSQCAINP 182
            INGQFPGP IRA+AGD + V L+NKL+T EG+VIHWHGIRQ  TPWADGTAS+SQC INP
Sbjct: 66   INGQFPGPTIRARAGDIVHVQLTNKLHT-EGVVIHWHGIRQKGTPWADGTASISQCVINP 124

Query: 183  GATFQYRFIVDRAGTYFYHGHYEMQRAAGLYGSLIVDVADGKIEPFPYHGEINLLLSDWW 362
            G TF YRF VDRAGTYFYHGHY MQR+AGLYGS+IVDVA+G+ EPF Y GE +LLLSDWW
Sbjct: 125  GETFDYRFTVDRAGTYFYHGHYGMQRSAGLYGSMIVDVAEGEKEPFHYDGEFDLLLSDWW 184

Query: 363  HQSAHDQEVALSSNPFRWIGEPQSILINGRGQYNCSMSAKYDNTT-NFCNIYGRPSDQCE 539
            H+S H QEV LSS P RWI EPQ++LINGRGQY CS++A + N++ + CNI G   +Q  
Sbjct: 185  HKSVHHQEVGLSSRPMRWINEPQALLINGRGQYGCSLAAHHGNSSFSQCNITGH--EQWA 242

Query: 540  PYVLSVEPRKFYRLRIASTTSLASLNLVIANHRMLLVEADGNYLQLKYVTNLDIYSGESY 719
            PY+L V+P K YR+RIASTT+LASLNL I NH+M++VEADGNYLQ     +LDIYSGESY
Sbjct: 243  PYILHVDPNKTYRIRIASTTALASLNLAIGNHKMMVVEADGNYLQPFETNDLDIYSGESY 302

Query: 720  SVILNTGQDPSVNYWVSVSQRGRKPPLPPPALTILHYKPNSVFGIKPSSPPPLSPAWDDL 899
            SV+L T QD S NYW+S   RGR+P   P ALTIL+YK NS   + P SPPP++P WDD 
Sbjct: 303  SVLLKTSQDSSQNYWISFGVRGREPH-TPQALTILNYKTNSASKL-PLSPPPVTPRWDDY 360

Query: 900  AQSKNFTNKVLGLQVDGISLQPHPSAIPRPPVRHDRRIFLLNTQTKIDGYVKWSVNNISL 1079
            A SK F+NK+  L         H S IP+P   + RRI LLNTQ K++GY KWS+NN+SL
Sbjct: 361  AHSKAFSNKIKALD--------HKS-IPKPSPTYHRRIVLLNTQNKMNGYTKWSINNVSL 411

Query: 1080 VLPTTPYLGSLKFGLKNGFNATTPPETFPSTYDITKPPANPNSMYGSGVYHFKHMEIVDV 1259
             +P TPYLGS++FGL++ F+ T PPE FP  YD+ KPP NPN+  GSGVY   +   VDV
Sbjct: 412  SIPATPYLGSIRFGLRDAFDQTKPPEDFPGHYDVMKPPVNPNTTTGSGVYMLGYYSTVDV 471

Query: 1260 ILQNANVLGEGVSEIHPWHLHGHDFWVLGYGDGKFSEENDEKNLNLVDPPLRNTVVVFPY 1439
            ILQNAN L E VSEIHPWHLHGHDFWVLGYG+GKF+ ++DEK  NL +PP RNT V+FPY
Sbjct: 472  ILQNANALTENVSEIHPWHLHGHDFWVLGYGEGKFT-KSDEKKFNLKNPPYRNTAVIFPY 530

Query: 1440 GWTAIRFIADNPGVWPFHCHIEPHLHMGMGVIFAVAVDKIKQIPDGVLDCGLT 1598
            GWTA+RF+ADNPGVW FHCHIEPHLH+GMGV+FA  V  + +IP   L CGLT
Sbjct: 531  GWTALRFVADNPGVWAFHCHIEPHLHLGMGVVFAEGVQHVAKIPKEALSCGLT 583