BLASTX nr result
ID: Papaver23_contig00005384
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver23_contig00005384
(2179 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFB74617.1| cytochrome P450 [Papaver somniferum] 536 e-161
gb|AFB74614.1| cytochrome P450 [Papaver somniferum] gi|388571246... 380 e-107
gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver... 369 e-106
dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta] 350 5e-96
gb|AFB74616.1| cytochrome P450 [Papaver somniferum] 341 3e-95
>gb|AFB74617.1| cytochrome P450 [Papaver somniferum]
Length = 556
Score = 536 bits (1381), Expect(2) = e-161
Identities = 279/462 (60%), Positives = 336/462 (72%), Gaps = 11/462 (2%)
Frame = +3
Query: 480 LVVSSWEMVKECFTGNNDTAFSNRPIPLAFKTIFYACGGIDSYGLSSVPYGKYWRELRKV 659
LVVSSWEMVKECFTG ND FSNRP LA K +FY +SYG + PYGKYWRELRK+
Sbjct: 105 LVVSSWEMVKECFTGTNDKLFSNRPSSLAVKLMFY---DTESYGFA--PYGKYWRELRKI 159
Query: 660 CVHNLLSNQQLLKFRHLIISQVDTSFNKLYELCKNSEDN------QGMVRMDDWLAQLSF 821
H LLSNQQL KF+HL IS+VD SF KL+ELC N++ +VRMDDW A L+F
Sbjct: 160 STHKLLSNQQLEKFKHLRISEVDNSFKKLHELCSNNKQGGDTTYVASLVRMDDWFAYLTF 219
Query: 822 NVIGRIVCGFQSDPKTGAPSRVEQFKEVINEASYFMSTSPVSDNVPMLGWIDQLTGLTRN 1001
NVIGRIV GFQS+ GA + E++K I+E S M+T VSD VP LGWID+LTGLT
Sbjct: 220 NVIGRIVSGFQSNAVAGATNSQEKYKLAIDEVSNLMATFAVSDVVPRLGWIDRLTGLTGK 279
Query: 1002 MKHCGKKLDLVVESIIKDHRQKR-RFSRTK-GA---DEQDDFIDICLSIMEQPQLPGNNS 1166
MK+CGKKLD VV ++DHRQK+ + SR GA E++DFID+CLSIMEQ Q+PGN+
Sbjct: 280 MKNCGKKLDAVVGDAVEDHRQKKLKISRNNTGALTEHEEEDFIDVCLSIMEQSQIPGNH- 338
Query: 1167 PPQIPIKSIVLDMIGGGXXXXXXXXXXXXXXXXNNPHVLDKAKQEVDAHFRKKRRSTXXX 1346
P+I +KSI LDM+ GG N+P +LDKAK+EVD +F KK+ S
Sbjct: 339 -PEISVKSIALDMLSGGSDTTKLIMTWTLSLLLNHPDILDKAKEEVDTYFGKKKISDNTP 397
Query: 1347 XXXXXXXXXIRNLVYIQAIIKESMRLYPASPVVERLSGEDCVVGGFHVPAGTRLWANVWK 1526
+ NLVYIQAIIKESMRLYPAS ++ER++ +DC VGGFHVPAGTRLW NVWK
Sbjct: 398 VVDAAD---VPNLVYIQAIIKESMRLYPASTLMERMTSDDCDVGGFHVPAGTRLWVNVWK 454
Query: 1527 MQRDPKVWDDPLVFRPERFLSDEQKMVDVRGQNYELLPFGAGRRICPGVSFSLDLMQLVL 1706
MQRDP+VW DPLVF PERFLS+++ MVDV+GQNYEL+PFG GRRICPG SF+L+++ LVL
Sbjct: 455 MQRDPRVWKDPLVFLPERFLSNDKGMVDVKGQNYELIPFGTGRRICPGASFALEVLHLVL 514
Query: 1707 TRLILEFEMKSPSGKVDMTATPGLMSYKVVPLDILLTHRRIN 1832
TRLILEFEMK+P GK+DM A PG KVVPLD+ LT R ++
Sbjct: 515 TRLILEFEMKAPEGKIDMRARPGFFHNKVVPLDVQLTPRTLD 556
Score = 60.5 bits (145), Expect(2) = e-161
Identities = 30/45 (66%), Positives = 34/45 (75%)
Frame = +1
Query: 277 SCALPISGLLHVFMNKNGLIHVTLGNMADKYGPIFSFPTGSHRTL 411
S A P+ G L +FMN+N L HVTLG+MADKYGPIFS G HRTL
Sbjct: 61 SGAWPVIGHLLLFMNENDLNHVTLGHMADKYGPIFSLRFGRHRTL 105
>gb|AFB74614.1| cytochrome P450 [Papaver somniferum] gi|388571246|gb|AFK73719.1|
cytochrome P450 [Papaver somniferum]
Length = 540
Score = 380 bits (976), Expect(2) = e-107
Identities = 213/463 (46%), Positives = 294/463 (63%), Gaps = 15/463 (3%)
Frame = +3
Query: 480 LVVSSWEMVKECFTGNNDTAFSNRPIPLAFKTIFYACGGIDSYGLSSVPYGKYWRELRKV 659
LVVS+WEMVK+CF ND FSNR LA K Y S G + PYG YWRELRK+
Sbjct: 93 LVVSNWEMVKQCFGNQNDKLFSNRQTTLAAK---YMLNQTTSSGFA--PYGPYWRELRKI 147
Query: 660 CVHNLLSNQQLLKFRHLIISQVDTSFNKLYELCKNSEDNQG-MVRMDDWLAQLSFNVIGR 836
V LLS Q L ++HL I ++D SF+KL ELC N+ ++RMD+W A+L+FNVI R
Sbjct: 148 MVQQLLSKQSLESWKHLKIKEMDASFSKLNELCNNNGTGTATLIRMDEWFAELTFNVIAR 207
Query: 837 IVCGFQSDPKTGA------PSRVEQFKEVINEASYFMSTSPVSDNVPMLGWIDQLTGLTR 998
V G+QS ++ A S+ E++K+ + EA + MS VSD P L W+D+L GL R
Sbjct: 208 NVFGYQSGGRSTALTNGDTESKGERYKKTLEEALHLMSIFAVSDIFPSLEWVDRLRGLIR 267
Query: 999 NMKHCGKKLDLVVESIIKDHRQKRRFSRTK---GADEQDDFIDICLSIMEQPQLPGNNSP 1169
NMK G +L+ + +I++HRQKR S +K G ++ DF+D+ LS+ E+ QLPG++
Sbjct: 268 NMKRFGDELNSIAGCLIEEHRQKRLQSVSKSDKGVGDEQDFVDVLLSVAEKSQLPGDD-- 325
Query: 1170 PQIPIKSIVLDMIGGGXXXXXXXXXXXXXXXXNNPHVLDKAKQEVDAHFRKKRRSTXXXX 1349
P + IKS++L+++ GG N+PHVL KAK+E+D H K R
Sbjct: 326 PDLVIKSMILEIVSGGSETTSSTLTWALCLLLNHPHVLKKAKEELDTHVGKDRHVEESDT 385
Query: 1350 XXXXXXXXIRNLVYIQAIIKESMRLYPASPVVERLSGEDCVVGGFHVPAGTRLWANVWKM 1529
LVYI AIIKESMRLYP +++RL+ E+C VGGFHVPAG RL+ NVWK+
Sbjct: 386 P---------KLVYINAIIKESMRLYPNGAMLDRLALEECEVGGFHVPAGGRLFVNVWKI 436
Query: 1530 QRDPKVWDDPLVFRPER-FLSDEQKM-VDVRGQNYELLPFGAGRRICPGVSFSLDLMQLV 1703
QRDP VW++PL F+PER FLS+ +KM VD +G N+E +PFG GRR+C G+ ++ +++ LV
Sbjct: 437 QRDPSVWENPLEFKPERWFLSNGEKMDVDYKGHNHEFIPFGIGRRMCAGMLWASEVIHLV 496
Query: 1704 LTRLILEFEMK--SPSGKVDMTATPGL-MSYKVVPLDILLTHR 1823
L RLI F+MK S +GKVDM G+ + +K PL++++ R
Sbjct: 497 LPRLIHGFDMKAASANGKVDMAEMAGMVICFKKTPLEVMVNPR 539
Score = 38.5 bits (88), Expect(2) = e-107
Identities = 18/43 (41%), Positives = 25/43 (58%)
Frame = +1
Query: 283 ALPISGLLHVFMNKNGLIHVTLGNMADKYGPIFSFPTGSHRTL 411
A P+ G L + MN ++ LG+MADKYGP F+ G+ L
Sbjct: 51 AWPLIGHLRLLMNDKEPLYRALGSMADKYGPAFNIRLGNQEVL 93
>gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver somniferum]
Length = 526
Score = 369 bits (947), Expect(2) = e-106
Identities = 211/458 (46%), Positives = 279/458 (60%), Gaps = 10/458 (2%)
Frame = +3
Query: 480 LVVSSWEMVKECFTGNNDTAFSNRPIPLAFKTIFYACGGIDSYGLSSVPYGKYWRELRKV 659
LVVS WE+VKECFT ND FSNRP L K +FY D+ + PYG YWR+LRK+
Sbjct: 92 LVVSDWEIVKECFT-TNDKLFSNRPGTLGIKLMFY-----DADSVGYAPYGAYWRDLRKI 145
Query: 660 CVHNLLSNQQLLKFRHLIISQVDTSFNKLYELCKNSEDNQGM--VRMDDWLAQLSFNVIG 833
LLSN ++ +HL S+V++ F LY N E + VRMD WL L+FNV+
Sbjct: 146 STLKLLSNHRIDTIKHLRSSEVESCFESLYSQWGNGEKSGEFAPVRMDSWLGDLTFNVVA 205
Query: 834 RIVCG---FQSDPKTGAPSRVEQFKEVINEASYFMSTSPVSDNVPMLGWIDQLTGLTRNM 1004
RIV G F ++ GA +++K ++EA M SD +P L W+D L GL R M
Sbjct: 206 RIVAGKKNFSANGDVGA----QRYKAAMDEAMRLMRFFAFSDVIPSLSWLDNLRGLVREM 261
Query: 1005 KHCGKKLDLVVESIIKDHRQKRRFSRTKGADEQ--DDFIDICLSIMEQPQLPGNNSPPQI 1178
K C ++D ++ + +++HR KR G + Q DFID+CL IME LPG++ P +
Sbjct: 262 KKCASEIDSIMATWVEEHRVKRN----SGGNSQLEHDFIDVCLDIMEHSSLPGDD--PDL 315
Query: 1179 PIKSIVLDMIGGGXXXXXXXXXXXXXXXXNNPHVLDKAKQEVDAHFRKKRRSTXXXXXXX 1358
+KS LDMI GG N+P VL KAK+E++ K R+
Sbjct: 316 VVKSTCLDMILGGSDTTTVTLTWAMSLLLNHPQVLQKAKEELETQVGKNRQ--------- 366
Query: 1359 XXXXXIRNLVYIQAIIKESMRLYPASPVVERLSGEDCVVGGFHVPAGTRLWANVWKMQRD 1538
I NL +IQAIIKE+MRLYPA P++ER + EDC V G+ VPAGTRL NVWKMQRD
Sbjct: 367 VDDSDIPNLPFIQAIIKETMRLYPAGPLIERRTMEDCEVAGYQVPAGTRLLVNVWKMQRD 426
Query: 1539 PKVW-DDPLVFRPERFLSDEQKMVDVRGQNYELLPFGAGRRICPGVSFSLDLMQLVLTRL 1715
V+ DPL FRP+RFL+ VD++GQ+YEL+PFGAGRRICPGVSF++ LM LVL RL
Sbjct: 427 GNVYKGDPLEFRPDRFLTSNAD-VDLKGQHYELIPFGAGRRICPGVSFAVQLMHLVLARL 485
Query: 1716 ILEFEMKS--PSGKVDMTATPGLMSYKVVPLDILLTHR 1823
+ EFE+ + P KVDM + GL+ YK++PL++L+ R
Sbjct: 486 LHEFEITTVEPETKVDMAESGGLLCYKIMPLEVLIKPR 523
Score = 45.8 bits (107), Expect(2) = e-106
Identities = 23/41 (56%), Positives = 27/41 (65%)
Frame = +1
Query: 289 PISGLLHVFMNKNGLIHVTLGNMADKYGPIFSFPTGSHRTL 411
PI G LH+F N L H LG MAD YGP+F+ GSH+TL
Sbjct: 53 PIMGHLHLF-NDGELTHRKLGAMADTYGPVFNIRFGSHKTL 92
>dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 534
Score = 350 bits (897), Expect(2) = 5e-96
Identities = 207/461 (44%), Positives = 281/461 (60%), Gaps = 13/461 (2%)
Frame = +3
Query: 480 LVVSSWEMVKECFTGNNDTAFSNRPIPLAFKTIFYACGGIDSYGLSSVPYGKYWRELRKV 659
LVVSSWE+VK CFT ND FS+R + A K +FY D +S P+G YWRELRK+
Sbjct: 92 LVVSSWELVKACFT-TNDKFFSSRLVNKAIKYMFY-----DQKTISFAPHGPYWRELRKM 145
Query: 660 CVHNLLSNQQLLKFRHLIISQVDTSFNKLYELC-KNSEDNQG-MVRMDDWLAQLSFNVIG 833
NLLSN++L +H IS++D KLYEL K ++N G +V M W +SFNV+
Sbjct: 146 ITLNLLSNERLKMLKHQRISEMDACLKKLYELSTKRKDENAGVLVDMSKWFGDISFNVVT 205
Query: 834 RIVCGFQS-DPKTGAPSRVEQFKEVINEASYFMSTSPVSDNVPMLGWIDQLTGLTRNMKH 1010
RIV G PKT E++ V+ EA M SD +P LGW+D+L G+ +K
Sbjct: 206 RIVAGKHIFGPKT------ERYMNVMEEARRLMDVMVFSDVIPYLGWLDRLRGVDSEIKR 259
Query: 1011 CGKKLDLVVESIIKDHRQKRRFSRTKGA--------DEQDDFIDICLSIMEQPQLPGNNS 1166
K+LD +ES + +HRQKR S + G +E+ DFIDI LSI+ + +L G++
Sbjct: 260 TAKELDSALESWVDEHRQKR-VSISAGIGGIVNITEEEEIDFIDIMLSIIAKNKLLGDD- 317
Query: 1167 PPQIPIKSIVLDMIGGGXXXXXXXXXXXXXXXXNNPHVLDKAKQEVDAHFRKKRRSTXXX 1346
P IK+IV +M NN VL +A+ E+DA K+R+
Sbjct: 318 -PGTLIKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQCELDAQVGKERQ----- 371
Query: 1347 XXXXXXXXXIRNLVYIQAIIKESMRLYPASPVVERLSGEDCVVGGFHVPAGTRLWANVWK 1526
I L YIQAI+KESMRLYP P++ER + EDC VG F +PAGTRLW N+WK
Sbjct: 372 ----VEDSDINTLPYIQAIVKESMRLYPPGPIIERETTEDCDVGDFRIPAGTRLWINLWK 427
Query: 1527 MQRDPKVW-DDPLVFRPERFLSDEQKMVDVRGQNYELLPFGAGRRICPGVSFSLDLMQLV 1703
+QRDP VW +DP F+PERFL+ +D++GQ++EL+PFG+GRR+CPGVSFSL +M LV
Sbjct: 428 LQRDPNVWPNDPQEFQPERFLNGHAD-IDMKGQHFELIPFGSGRRMCPGVSFSLQVMHLV 486
Query: 1704 LTRLILEFEMKSPS-GKVDMTATPGLMSYKVVPLDILLTHR 1823
L R+I FE+K+P+ +DM+ T G++S+K PL++LLT R
Sbjct: 487 LARIIHGFELKTPTDADIDMSTTLGMISWKATPLEVLLTPR 527
Score = 30.4 bits (67), Expect(2) = 5e-96
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +1
Query: 337 HVTLGNMADKYGPIFSFPTGSHRTL 411
++ LG +AD+YGP FS G H L
Sbjct: 68 YIVLGELADQYGPAFSIQFGVHPIL 92
>gb|AFB74616.1| cytochrome P450 [Papaver somniferum]
Length = 554
Score = 341 bits (874), Expect(2) = 3e-95
Identities = 198/465 (42%), Positives = 279/465 (60%), Gaps = 17/465 (3%)
Frame = +3
Query: 480 LVVSSWEMVKECFTGNNDTAFSNRPIPLAFKTIFYACGGIDSYGLSSVPYGKYWRELRKV 659
LVVS+WEMVKECFT ND +FSNRP L+ K + D+ + PYG YWRE+RK+
Sbjct: 102 LVVSNWEMVKECFT-TNDKSFSNRPSTLSTKYMLN-----DTNSVVFSPYGTYWREMRKI 155
Query: 660 CVHNLL-SNQQLLKFRHLIISQVDTSFNKLYELCKNSEDNQGM-VRMDDWLAQLSFNVIG 833
V LL SNQ+ ++L ++D SF KL +LC N G VRMD+WLA + FN+I
Sbjct: 156 LVQKLLISNQRSEALKNLKTKEIDNSFVKLNDLCNNDVSGGGTKVRMDEWLADMMFNIIA 215
Query: 834 RIVCGFQSD----PKTGAPSR-VEQFKEVINEASYFMSTS-PVSDNVPMLGWIDQLTGLT 995
RI G+QS P S+ VE++K+ ++E ++T VSD P L +ID+L GL
Sbjct: 216 RITFGYQSGGGDAPGASTTSKNVERYKKTLDEMFVVLATRFAVSDIFPSLEFIDRLRGLV 275
Query: 996 RNMKHCGKKLDLVVESIIKDHRQKRRFSRT-------KGADEQDDFIDICLSIMEQPQLP 1154
++MK G +L+ + I++HRQKRR S + + ++ DFID+ LSIM+Q +LP
Sbjct: 276 KDMKILGDELNSIAGCFIEEHRQKRRESLSSLLSLSNESVGDEQDFIDVLLSIMDQSRLP 335
Query: 1155 GNNSPPQIPIKSIVLDMIGGGXXXXXXXXXXXXXXXXNNPHVLDKAKQEVDAHFRKKRRS 1334
G++ P IK ++L+ GG N+P+VL +A++E+D H ++
Sbjct: 336 GDD--PDFIIKIMILEAFAGGTDSLSATLTWVLSLLLNHPNVLKRAREEIDRHVENGKQ- 392
Query: 1335 TXXXXXXXXXXXXIRNLVYIQAIIKESMRLYPASPVVERLSGEDCVVGGFHVPAGTRLWA 1514
I L YI AIIKE+MRLYP + ER + E+C VG F+VPAGTRL
Sbjct: 393 --------VEVSDIPKLGYIDAIIKETMRLYPVGALSERYTTEECEVGRFNVPAGTRLLV 444
Query: 1515 NVWKMQRDPKVWDDPLVFRPERFLSDEQKMVDVRGQNYELLPFGAGRRICPGVSFSLDLM 1694
N+WK+ RDP VW++P F+PERFL ++ VD+ GQNYEL+PFGAGRR+CP + SL M
Sbjct: 445 NIWKIHRDPSVWENPSDFQPERFLCSDKVGVDLYGQNYELIPFGAGRRVCPAIVSSLQTM 504
Query: 1695 QLVLTRLILEFEMKSPS--GKVDMTATPGLMSYKVVPLDILLTHR 1823
L RLI +EMKS S GKV+M + +K+ PL+++++ R
Sbjct: 505 HYALARLIQGYEMKSASLDGKVNMEEMIAMSCHKMSPLEVIISPR 549
Score = 36.6 bits (83), Expect(2) = 3e-95
Identities = 18/45 (40%), Positives = 26/45 (57%)
Frame = +1
Query: 277 SCALPISGLLHVFMNKNGLIHVTLGNMADKYGPIFSFPTGSHRTL 411
S A P G L +FM ++ + TLG M+DKYG +F+ G+ L
Sbjct: 58 SGAWPFIGHLKLFMKQDTQFYRTLGTMSDKYGSVFTLRLGNQAIL 102