BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver22_contig00036565
         (464 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       100   1e-19
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...    98   8e-19
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...    97   1e-18
sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Sh...    96   2e-18
gb|AFB74615.1| cytochrome P450 [Papaver somniferum]                    96   3e-18

>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  100 bits (249), Expect = 1e-19
 Identities = 55/136 (40%), Positives = 84/136 (61%)
 Frame = -1

Query: 422 FGVEFEDKDGFVENIDTVMEETIGLSGHARLVDVFEFTRYIPGFRLLFKEAYELKHKIEE 243
           FG  F D D FVE++   +E+ + LSG+ARL + F + +Y+P  + + +EAY  K++IEE
Sbjct: 195 FGPTF-DNDKFVESVLYEIEDVLRLSGYARLTEAFYYAKYLPSQKKVAREAYLAKYRIEE 253

Query: 242 TIRPYIRRYKSSTNCFLQFLLSQEDYSEEVVIFNLFEQFLLXXXXXXXXXSWALAYLIHN 63
            +RP +      TN +L FL SQ +YS+E++IF  FE FLL         +WALAYLIH 
Sbjct: 254 LMRPLLSS-NPPTNSYLYFLQSQ-NYSKEIIIFCTFELFLLGVDSTSSTATWALAYLIHE 311

Query: 62  EKIQQKFYAEVQEITG 15
           + +Q+K   +++   G
Sbjct: 312 QAVQEKLNEDIRMTIG 327


>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
           Full=(S)-tetrahydrocolumbamine oxidase
           (methylenedioxy-bridge-forming); AltName:
           Full=Cytochrome P450 719A1 gi|15822820|dbj|BAB68769.1|
           methylenedioxy bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score = 97.8 bits (242), Expect = 8e-19
 Identities = 52/136 (38%), Positives = 84/136 (61%)
 Frame = -1

Query: 422 FGVEFEDKDGFVENIDTVMEETIGLSGHARLVDVFEFTRYIPGFRLLFKEAYELKHKIEE 243
           FG  F+D   F+E++   +E+ I +SG+ARL + F + +Y+P  +   +EA+ +K ++EE
Sbjct: 195 FGQAFDDNK-FIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCRVEE 253

Query: 242 TIRPYIRRYKSSTNCFLQFLLSQEDYSEEVVIFNLFEQFLLXXXXXXXXXSWALAYLIHN 63
            +RP +   K  TN +L FLLSQ ++ EEV+IF +FE +LL         +WALAYLI  
Sbjct: 254 LVRPLLSS-KPPTNSYLYFLLSQ-NFEEEVIIFCIFELYLLGVDSTSSTTTWALAYLIRE 311

Query: 62  EKIQQKFYAEVQEITG 15
           +  Q+K Y +++   G
Sbjct: 312 QGAQEKLYQDIRMTLG 327


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score = 97.4 bits (241), Expect = 1e-18
 Identities = 53/136 (38%), Positives = 83/136 (61%)
 Frame = -1

Query: 422 FGVEFEDKDGFVENIDTVMEETIGLSGHARLVDVFEFTRYIPGFRLLFKEAYELKHKIEE 243
           FG  F+D D FVE++   +++ I +SG+ARL + F + +Y+P  +   +EAY +K ++E+
Sbjct: 196 FGQTFDD-DKFVESMHFEIDDIIRISGYARLAEAFYYAKYLPSHKKAEREAYLVKCRVED 254

Query: 242 TIRPYIRRYKSSTNCFLQFLLSQEDYSEEVVIFNLFEQFLLXXXXXXXXXSWALAYLIHN 63
            + P +      TN +L FLLSQ  +SEEV+IF +FE +LL         +WALAYLI  
Sbjct: 255 LVSPLLSS-NPPTNSYLYFLLSQR-FSEEVIIFCIFELYLLGVDSTSSTTTWALAYLIRE 312

Query: 62  EKIQQKFYAEVQEITG 15
           + IQ+K Y + +   G
Sbjct: 313 QAIQEKLYQDTRMTLG 328


>sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Short=STS; AltName:
           Full=Cytochrome P450 719A13 gi|148841092|gb|ABR14721.1|
           stylopine synthase [Argemone mexicana]
          Length = 504

 Score = 96.3 bits (238), Expect = 2e-18
 Identities = 49/135 (36%), Positives = 79/135 (58%)
 Frame = -1

Query: 422 FGVEFEDKDGFVENIDTVMEETIGLSGHARLVDVFEFTRYIPGFRLLFKEAYELKHKIEE 243
           +G +F D D +VE +   +EE I +SG+ARL + F + +Y+P  +   +E  +   +++ 
Sbjct: 207 YGQDF-DNDEYVEEMHHTIEELIRVSGYARLAEAFYYAKYLPSHKKAVREVLQANQRVQN 265

Query: 242 TIRPYIRRYKSSTNCFLQFLLSQEDYSEEVVIFNLFEQFLLXXXXXXXXXSWALAYLIHN 63
            +RP +    S TN +L FL SQ +Y +EV+IF +FE +LL         +WALAYLI  
Sbjct: 266 LVRPLLS-LNSPTNTYLHFLRSQ-NYEDEVIIFAIFEAYLLGVDSTSSTTAWALAYLIRE 323

Query: 62  EKIQQKFYAEVQEIT 18
             +Q+K Y E++  T
Sbjct: 324 PNVQEKLYEELKNFT 338


>gb|AFB74615.1| cytochrome P450 [Papaver somniferum]
          Length = 486

 Score = 95.9 bits (237), Expect = 3e-18
 Identities = 51/135 (37%), Positives = 83/135 (61%)
 Frame = -1

Query: 422 FGVEFEDKDGFVENIDTVMEETIGLSGHARLVDVFEFTRYIPGFRLLFKEAYELKHKIEE 243
           +G +F+D D +VE++  V+E  I +SG+A+L +VF + +Y+PG +     A E K ++  
Sbjct: 194 YGQDFDD-DKYVEDMHDVIEFLIRISGYAQLAEVFYYAKYLPGHKRAVTGAEEAKRRVIA 252

Query: 242 TIRPYIRRYKSSTNCFLQFLLSQEDYSEEVVIFNLFEQFLLXXXXXXXXXSWALAYLIHN 63
            +RP+++    +TN +L FL SQ  Y EEV+IF +FE +LL         +WALA+LI  
Sbjct: 253 LVRPFLQS-NPATNTYLHFLKSQL-YPEEVIIFAIFEAYLLGVDSTSSTTAWALAFLIRE 310

Query: 62  EKIQQKFYAEVQEIT 18
             +Q+K Y E++  T
Sbjct: 311 PSVQEKLYQELKNFT 325