BLASTX nr result
ID: Papaver22_contig00015656
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver22_contig00015656
(1250 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin... 240 e-123
sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin... 237 e-117
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi... 235 e-117
sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydropro... 231 e-114
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica] 189 6e-94
>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT gi|226897702|gb|ACO90222.1|
tetrahydroprotoberberine N-methyltransferase
[Eschscholzia californica]
Length = 350
Score = 240 bits (612), Expect(2) = e-123
Identities = 119/176 (67%), Positives = 143/176 (81%)
Frame = -1
Query: 1196 SGGAKILERLVKGEIGDEELKELIRIRFEKRLQCGYKPTPQDQLASEMAFIKSLKEMKMS 1017
S +I+ RL+KGEI DEELK+LIR ++++R++ GYKPT + QLA + FIK LKEM MS
Sbjct: 3 SSAGEIMGRLMKGEIEDEELKKLIRHQWDRRIEWGYKPTHEKQLAFNLDFIKGLKEMVMS 62
Query: 1016 GELEAVNTELYELPTAAVEATLGSTLKQSACYFKEESMTIDEAEIAAYELDCERAQIKDG 837
GE++ +N E YELPTA +EA G T+KQS CYFK+E+ TIDEAE AA+EL CERAQIKDG
Sbjct: 63 GEIDTMNKETYELPTAFLEAVFGKTVKQSCCYFKDENSTIDEAEEAAHELYCERAQIKDG 122
Query: 836 QTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILA 669
QT+LDIGCG GGLVL+IA+KYKNCHVTGLTNS Q NYIE Q EKL L+NVDVI A
Sbjct: 123 QTVLDIGCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFA 178
Score = 228 bits (581), Expect(2) = e-123
Identities = 113/182 (62%), Positives = 137/182 (75%)
Frame = -3
Query: 621 NVDVILADVTKYEFENEKKFDRILVVEAIEHMKNIQQFLKKISKWMKDEDSFLFVVHLCH 442
NVDVI ADVTK F+ +K +DRILVVE IEHMKNIQ F+KK+S WM EDS LFV H+ H
Sbjct: 172 NVDVIFADVTK--FDTDKTYDRILVVETIEHMKNIQLFMKKLSTWMT-EDSLLFVDHISH 228
Query: 441 KAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVVDQWLLSGTHMARSQEE 262
K F+ +FEALDEDDWY+ ++ P+G VT LS+S LLYFQDDVS +D W+++G HMARS E
Sbjct: 229 KTFNHNFEALDEDDWYSGFIFPKGCVTILSSSTLLYFQDDVSALDHWVVNGMHMARSVEA 288
Query: 261 WFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQFSYNNGEEWMISHYLFK 82
W K+ +EAA + L GLGS+EA NQV +TF +G QFSYNNGEEWMI+ LFK
Sbjct: 289 WRKKLDETIEAAREILEPGLGSKEAVNQVITHIRTFCIGGYEQFSYNNGEEWMITQILFK 348
Query: 81 KK 76
KK
Sbjct: 349 KK 350
>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT gi|67764091|gb|AAY79177.1|
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-cis-
N-methyltransferase [Papaver somniferum]
Length = 358
Score = 237 bits (604), Expect(2) = e-117
Identities = 120/178 (67%), Positives = 141/178 (79%)
Frame = -1
Query: 1202 KKSGGAKILERLVKGEIGDEELKELIRIRFEKRLQCGYKPTPQDQLASEMAFIKSLKEMK 1023
KK + L RL+KGEI DEELK+LI+ +FEKRLQ GYK + Q+QL+ + FIKSLK+M+
Sbjct: 8 KKESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLKKME 67
Query: 1022 MSGELEAVNTELYELPTAAVEATLGSTLKQSACYFKEESMTIDEAEIAAYELDCERAQIK 843
MSGE+E +N E YELP+ +EA G T+KQS CYF ES TIDEAE AA+EL CERAQIK
Sbjct: 68 MSGEIETMNKETYELPSEFLEAVFGKTVKQSMCYFTHESATIDEAEEAAHELYCERAQIK 127
Query: 842 DGQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILA 669
DGQT+LDIGCG GGLVL+IAQKYKNCHVTGLTNS Q NY+ Q EKL L+NVD ILA
Sbjct: 128 DGQTVLDIGCGQGGLVLYIAQKYKNCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILA 185
Score = 213 bits (541), Expect(2) = e-117
Identities = 105/183 (57%), Positives = 134/183 (73%), Gaps = 1/183 (0%)
Frame = -3
Query: 621 NVDVILADVTKYEFENEKKFDRILVVEAIEHMKNIQQFLKKISKWMKDEDSFLFVVHLCH 442
NVD ILADVT+YE ++K +DR+L++EAIEHMKN+Q F+KK+S WM E S LFV H+CH
Sbjct: 179 NVDAILADVTQYE--SDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTKE-SLLFVDHVCH 235
Query: 441 KAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVVDQWLLSGTHMARSQEE 262
K F+ FEA+DEDDWY+ ++ P G T L+A++LLYFQDDVSVVD W+++G HMARS +
Sbjct: 236 KTFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDI 295
Query: 261 WFKRFKINLEAASKALTEGL-GSEEAANQVNIQYKTFFMGYVVQFSYNNGEEWMISHYLF 85
W K N+EAA + L GL GS E N V +TF MG QFS NNG+EWM++ LF
Sbjct: 296 WRKALDKNMEAAKEILLPGLGGSHETVNGVVTHIRTFCMGGYEQFSMNNGDEWMVAQLLF 355
Query: 84 KKK 76
KKK
Sbjct: 356 KKK 358
>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 gi|226897732|gb|ACO90237.1|
tetrahydroprotoberberine N-methyltransferase [Papaver
bracteatum]
Length = 358
Score = 235 bits (600), Expect(2) = e-117
Identities = 119/178 (66%), Positives = 141/178 (79%)
Frame = -1
Query: 1202 KKSGGAKILERLVKGEIGDEELKELIRIRFEKRLQCGYKPTPQDQLASEMAFIKSLKEMK 1023
KK + L RL+KGEI DEELK+LI+ +FEKRLQ GYK + Q+QL+ + FIKSLK+M+
Sbjct: 8 KKESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLKKME 67
Query: 1022 MSGELEAVNTELYELPTAAVEATLGSTLKQSACYFKEESMTIDEAEIAAYELDCERAQIK 843
MSGE+E +N E YELP+ +EA G T+KQS CYFK ES TIDEAE AA+EL CERAQIK
Sbjct: 68 MSGEIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATIDEAEEAAHELYCERAQIK 127
Query: 842 DGQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILA 669
DGQT+LDIGCG GGLVL+IA+KYK CHVTGLTNS Q NY+ Q EKL L+NVD ILA
Sbjct: 128 DGQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILA 185
Score = 213 bits (543), Expect(2) = e-117
Identities = 105/183 (57%), Positives = 136/183 (74%), Gaps = 1/183 (0%)
Frame = -3
Query: 621 NVDVILADVTKYEFENEKKFDRILVVEAIEHMKNIQQFLKKISKWMKDEDSFLFVVHLCH 442
NVD ILADVT+YE ++K +DR+L++EAIEHMKN+Q F+KK+S WM +E S LFV H+CH
Sbjct: 179 NVDAILADVTQYE--SDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEE-SLLFVDHVCH 235
Query: 441 KAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVVDQWLLSGTHMARSQEE 262
K F+ FEA+DEDDWY+ ++ P G T L+A++LLYFQDDVSVVD W+++G HMARS +
Sbjct: 236 KTFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDI 295
Query: 261 WFKRFKINLEAASKALTEGL-GSEEAANQVNIQYKTFFMGYVVQFSYNNGEEWMISHYLF 85
W K N+EAA + L GL GS EA N V +TF MG QFS N+G+EWM++ LF
Sbjct: 296 WRKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLF 355
Query: 84 KKK 76
KKK
Sbjct: 356 KKK 358
>sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2
gi|226897730|gb|ACO90236.1| putative N-methyltransferase
[Papaver bracteatum]
Length = 358
Score = 231 bits (588), Expect(2) = e-114
Identities = 117/178 (65%), Positives = 138/178 (77%)
Frame = -1
Query: 1202 KKSGGAKILERLVKGEIGDEELKELIRIRFEKRLQCGYKPTPQDQLASEMAFIKSLKEMK 1023
KK + L RL++GEI DEELK+LI+ + EKRLQ GYK + Q+QL+ + FI SLK+M
Sbjct: 8 KKESAEETLGRLLRGEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFINSLKKMG 67
Query: 1022 MSGELEAVNTELYELPTAAVEATLGSTLKQSACYFKEESMTIDEAEIAAYELDCERAQIK 843
MSG++EA E+YELPT EA G ++K S CYFK ES TIDEAE A++EL CERAQIK
Sbjct: 68 MSGQVEAFTNEVYELPTECFEAAYGKSMKLSGCYFKHESSTIDEAEEASHELYCERAQIK 127
Query: 842 DGQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILA 669
DGQT+LDIGCG GGLVL++AQKYKNCHVTGLTNS EQ NYI Q EKL L NVDVILA
Sbjct: 128 DGQTVLDIGCGQGGLVLYVAQKYKNCHVTGLTNSKEQVNYILKQAEKLGLRNVDVILA 185
Score = 209 bits (531), Expect(2) = e-114
Identities = 104/183 (56%), Positives = 132/183 (72%), Gaps = 1/183 (0%)
Frame = -3
Query: 621 NVDVILADVTKYEFENEKKFDRILVVEAIEHMKNIQQFLKKISKWMKDEDSFLFVVHLCH 442
NVDVILADVT+YE ++K +DRILV+ +EHMKN+Q F+KK+S WM EDS LFV H CH
Sbjct: 179 NVDVILADVTQYE--SDKTYDRILVIGVVEHMKNMQLFIKKLSTWMA-EDSLLFVDHSCH 235
Query: 441 KAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVVDQWLLSGTHMARSQEE 262
K F+ FEALDEDDWY+ Y+ P G T+LSA +LLYFQDDVSVVD W+++G H AR+ +
Sbjct: 236 KTFNHFFEALDEDDWYSGYIFPPGCATFLSADSLLYFQDDVSVVDHWVVNGMHFARTVDA 295
Query: 261 WFKRFKINLEAASKALTEGL-GSEEAANQVNIQYKTFFMGYVVQFSYNNGEEWMISHYLF 85
W K+ N+EA + L GL G+ EA N V +T +G VQFS N+G+EWM + LF
Sbjct: 296 WRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDGDEWMNAQLLF 355
Query: 84 KKK 76
KKK
Sbjct: 356 KKK 358
>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
Length = 358
Score = 189 bits (481), Expect(2) = 6e-94
Identities = 91/182 (50%), Positives = 122/182 (67%)
Frame = -3
Query: 621 NVDVILADVTKYEFENEKKFDRILVVEAIEHMKNIQQFLKKISKWMKDEDSFLFVVHLCH 442
NV+V LAD+T +E + +DRILV+E EHMKN + L+KIS+W+ +D LF+ H+CH
Sbjct: 180 NVEVKLADITTHEMA--ETYDRILVIELFEHMKNYELLLRKISEWIS-KDGLLFLEHICH 236
Query: 441 KAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVVDQWLLSGTHMARSQEE 262
K F+ H+E LD+DDW+T YV P G++ SAS LYFQDDVSVV+ W LSG H +R+ EE
Sbjct: 237 KTFAYHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEE 296
Query: 261 WFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQFSYNNGEEWMISHYLFK 82
W KR NL+ +G+EE A ++ ++ F + + F YNNGEEWM SH LFK
Sbjct: 297 WLKRLDANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFK 356
Query: 81 KK 76
KK
Sbjct: 357 KK 358
Score = 182 bits (462), Expect(2) = 6e-94
Identities = 89/178 (50%), Positives = 128/178 (71%)
Frame = -1
Query: 1202 KKSGGAKILERLVKGEIGDEELKELIRIRFEKRLQCGYKPTPQDQLASEMAFIKSLKEMK 1023
KK+ ++L++L G + +++K+LIR +RLQ GYKPT ++Q+A SL++MK
Sbjct: 9 KKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNLTHSLRQMK 68
Query: 1022 MSGELEAVNTELYELPTAAVEATLGSTLKQSACYFKEESMTIDEAEIAAYELDCERAQIK 843
++ E+E ++++LYE+P ++ GS LK S CYFKE+S T+DEAEIA +L CERAQI+
Sbjct: 69 IATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQ 128
Query: 842 DGQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILA 669
DGQ++LD+GCG G L LH+AQKYKNC VT +TNS Q+ YIE + + NL NV+V LA
Sbjct: 129 DGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLA 186