BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver22_contig00010359
         (1707 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver...   662   0.0  
gb|AFB74617.1| cytochrome P450 [Papaver somniferum]                   477   e-132
dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]    471   e-130
gb|AFB74614.1| cytochrome P450 [Papaver somniferum] gi|388571246...   470   e-130
dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]       464   e-128

>gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver somniferum]
          Length = 526

 Score =  662 bits (1709), Expect = 0.0
 Identities = 339/537 (63%), Positives = 404/537 (75%), Gaps = 9/537 (1%)
 Frame = +3

Query: 60   QPISLLIAPLLLGIIVLCCEYGRKSKKTQKQEAPEAXXXXXXXXXXXXXXXXXXXXXXXX 239
            QPIS+ +      +I L   YGR++    K+ APEA                        
Sbjct: 20   QPISVALV-----VIALVWNYGRRNPT--KKLAPEASG---------------------- 50

Query: 240  XGRPIMGHLHLFKAMDSMHQKLGSMADEYGPAFTVRLGSHKTLVVSNWEMVKECFTTNDI 419
             GRPIMGHLHLF   +  H+KLG+MAD YGP F +R GSHKTLVVS+WE+VKECFTTND 
Sbjct: 51   -GRPIMGHLHLFNDGELTHRKLGAMADTYGPVFNIRFGSHKTLVVSDWEIVKECFTTNDK 109

Query: 420  FFSNHPSILSGKLLFYDVVSVGYAPYGAYWRELRKFSALKLLSSYRLETFKHLRTSEIES 599
             FSN P  L  KL+FYD  SVGYAPYGAYWR+LRK S LKLLS++R++T KHLR+SE+ES
Sbjct: 110  LFSNRPGTLGIKLMFYDADSVGYAPYGAYWRDLRKISTLKLLSNHRIDTIKHLRSSEVES 169

Query: 600  SFTFLYNQCKGDNL--QFASVRLDSWLGDLAFNVITRIVIGKKNFATN---GAERYKAAM 764
             F  LY+Q        +FA VR+DSWLGDL FNV+ RIV GKKNF+ N   GA+RYKAAM
Sbjct: 170  CFESLYSQWGNGEKSGEFAPVRMDSWLGDLTFNVVARIVAGKKNFSANGDVGAQRYKAAM 229

Query: 765  QEAMRLVTVFAFADVFPALSWLDNFTGLTRDLKKCAHEIDKVLSGWVEEHRSKRIGGNKS 944
             EAMRL+  FAF+DV P+LSWLDN  GL R++KKCA EID +++ WVEEHR KR  G  S
Sbjct: 230  DEAMRLMRFFAFSDVIPSLSWLDNLRGLVREMKKCASEIDSIMATWVEEHRVKRNSGGNS 289

Query: 945  LELEQDFIDSCLDNLQENSPLPGDDPDIVIKSTCLDMIMGGSDTLSVTLTWAISLLLNHP 1124
             +LE DFID CLD + E+S LPGDDPD+V+KSTCLDMI+GGSDT +VTLTWA+SLLLNHP
Sbjct: 290  -QLEHDFIDVCLD-IMEHSSLPGDDPDLVVKSTCLDMILGGSDTTTVTLTWAMSLLLNHP 347

Query: 1125 DALKRAQEELDTHVGKDRKVDESDLRNFTYIPAIIKETMRLYPVG-AVSRSTVEDCEVGG 1301
              L++A+EEL+T VGK+R+VD+SD+ N  +I AIIKETMRLYP G  + R T+EDCEV G
Sbjct: 348  QVLQKAKEELETQVGKNRQVDDSDIPNLPFIQAIIKETMRLYPAGPLIERRTMEDCEVAG 407

Query: 1302 YHVPAGTRLLVNLWKMHRDGNVYKDDPLEFRPERFLTSNADVDLKGQHYELLPFGIGRRM 1481
            Y VPAGTRLLVN+WKM RDGNVYK DPLEFRP+RFLTSNADVDLKGQHYEL+PFG GRR+
Sbjct: 408  YQVPAGTRLLVNVWKMQRDGNVYKGDPLEFRPDRFLTSNADVDLKGQHYELIPFGAGRRI 467

Query: 1482 CPAVSMAVQLLHLILARVIHEFEINT---QGKVDMRENMGFLLYKKTPLEVLIRPRI 1643
            CP VS AVQL+HL+LAR++HEFEI T   + KVDM E+ G L YK  PLEVLI+PR+
Sbjct: 468  CPGVSFAVQLMHLVLARLLHEFEITTVEPETKVDMAESGGLLCYKIMPLEVLIKPRL 524


>gb|AFB74617.1| cytochrome P450 [Papaver somniferum]
          Length = 556

 Score =  477 bits (1228), Expect = e-132
 Identities = 257/491 (52%), Positives = 331/491 (67%), Gaps = 27/491 (5%)
 Frame = +3

Query: 249  PIMGHLHLF-KAMDSMHQKLGSMADEYGPAFTVRLGSHKTLVVSNWEMVKECFT-TNDIF 422
            P++GHL LF    D  H  LG MAD+YGP F++R G H+TLVVS+WEMVKECFT TND  
Sbjct: 65   PVIGHLLLFMNENDLNHVTLGHMADKYGPIFSLRFGRHRTLVVSSWEMVKECFTGTNDKL 124

Query: 423  FSNHPSILSGKLLFYDVVSVGYAPYGAYWRELRKFSALKLLSSYRLETFKHLRTSEIESS 602
            FSN PS L+ KL+FYD  S G+APYG YWRELRK S  KLLS+ +LE FKHLR SE+++S
Sbjct: 125  FSNRPSSLAVKLMFYDTESYGFAPYGKYWRELRKISTHKLLSNQQLEKFKHLRISEVDNS 184

Query: 603  FTFLYNQCK-----GDNLQFAS-VRLDSWLGDLAFNVITRIVIGKKN----FATNGAERY 752
            F  L+  C      GD    AS VR+D W   L FNVI RIV G ++     ATN  E+Y
Sbjct: 185  FKKLHELCSNNKQGGDTTYVASLVRMDDWFAYLTFNVIGRIVSGFQSNAVAGATNSQEKY 244

Query: 753  KAAMQEAMRLVTVFAFADVFPALSWLDNFTGLTRDLKKCAHEIDKVLSGWVEEHRSKRI- 929
            K A+ E   L+  FA +DV P L W+D  TGLT  +K C  ++D V+   VE+HR K++ 
Sbjct: 245  KLAIDEVSNLMATFAVSDVVPRLGWIDRLTGLTGKMKNCGKKLDAVVGDAVEDHRQKKLK 304

Query: 930  -----GGNKSLELEQDFIDSCLDNLQENSPLPGDDPDIVIKSTCLDMIMGGSDTLSVTLT 1094
                  G  +   E+DFID CL ++ E S +PG+ P+I +KS  LDM+ GGSDT  + +T
Sbjct: 305  ISRNNTGALTEHEEEDFIDVCL-SIMEQSQIPGNHPEISVKSIALDMLSGGSDTTKLIMT 363

Query: 1095 WAISLLLNHPDALKRAQEELDTHVGKDR------KVDESDLRNFTYIPAIIKETMRLYPV 1256
            W +SLLLNHPD L +A+EE+DT+ GK +       VD +D+ N  YI AIIKE+MRLYP 
Sbjct: 364  WTLSLLLNHPDILDKAKEEVDTYFGKKKISDNTPVVDAADVPNLVYIQAIIKESMRLYPA 423

Query: 1257 GA-VSRSTVEDCEVGGYHVPAGTRLLVNLWKMHRDGNVYKDDPLEFRPERFLTSNAD-VD 1430
               + R T +DC+VGG+HVPAGTRL VN+WKM RD  V+K DPL F PERFL+++   VD
Sbjct: 424  STLMERMTSDDCDVGGFHVPAGTRLWVNVWKMQRDPRVWK-DPLVFLPERFLSNDKGMVD 482

Query: 1431 LKGQHYELLPFGIGRRMCPAVSMAVQLLHLILARVIHEFEINT-QGKVDMRENMGFLLYK 1607
            +KGQ+YEL+PFG GRR+CP  S A+++LHL+L R+I EFE+   +GK+DMR   GF   K
Sbjct: 483  VKGQNYELIPFGTGRRICPGASFALEVLHLVLTRLILEFEMKAPEGKIDMRARPGFFHNK 542

Query: 1608 KTPLEVLIRPR 1640
              PL+V + PR
Sbjct: 543  VVPLDVQLTPR 553


>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
          Length = 524

 Score =  471 bits (1211), Expect = e-130
 Identities = 238/477 (49%), Positives = 335/477 (70%), Gaps = 12/477 (2%)
 Frame = +3

Query: 249  PIMGHLHLFKAMDSMHQKLGSMADEYGPAFTVRLGSHKTLVVSNWEMVKECFTTNDIFFS 428
            P++GHLHLF + +  H+ L +MAD+YG AF ++ G H TLVVS+  +VKECFTTND  FS
Sbjct: 48   PVLGHLHLFGSGELPHKMLAAMADKYGSAFRMKFGKHTTLVVSDTRIVKECFTTNDTLFS 107

Query: 429  NHPSILSGKLLFYDVVSVGYAPYGAYWRELRKFSALKLLSSYRLETFKHLRTSEIESSFT 608
            N PS  + +L+ YD  SV + PYG+YWRE+RK S LKLLS++RL+  K +R SE+   F 
Sbjct: 108  NRPSTKAFQLMTYDNESVAFTPYGSYWREIRKISTLKLLSNHRLQAIKDVRASEVNVCFK 167

Query: 609  FLYNQCKGDNLQFASVRLD--SWLGDLAFNVITRIVIGKKNFAT------NGAERYKAAM 764
             LY+QCK  +   A + +D   W  +++ NV+ R+++G++NF +        A  YK  M
Sbjct: 168  TLYDQCKNPS-GSAPILIDMKKWFEEVSNNVVMRVIVGRQNFGSKIVQGEEEAIHYKKVM 226

Query: 765  QEAMRLVTVFAFADVFPALSWLDNFTGLTRDLKKCAHEIDKVLSGWVEEHRSKRIGGNKS 944
             E +RL ++  F+D  P L ++D F G    +K+ A ++D +L  W+EEHR K+   N  
Sbjct: 227  DELLRLASLSMFSDFAPLLGFVDIFQGNLSAMKRNAKKVDAILENWLEEHRKKK---NSV 283

Query: 945  LELEQDFIDSCLDNLQENSPLPGDDPDIVIKSTCLDMIMGGSDTLSVTLTWAISLLLNHP 1124
             E +QDF+D  L ++ E S L G D D VIK+TCL MIMGG+DT +V+LTW ISLL+N+ 
Sbjct: 284  AESQQDFMDVML-SIVEESKLSGHDADAVIKATCLAMIMGGTDTTAVSLTWIISLLMNNR 342

Query: 1125 DALKRAQEELDTHVGKDRKVDESDLRNFTYIPAIIKETMRLYPVGA-VSRSTVEDCEVGG 1301
             ALK+A+EELD  VGKDR+V++SDL+N  Y+ AI+KETMR+YP+G  + R T EDCE+ G
Sbjct: 343  HALKKAREELDALVGKDRQVEDSDLKNLVYMNAIVKETMRMYPLGTLLERETKEDCEIDG 402

Query: 1302 YHVPAGTRLLVNLWKMHRDGNVYKDDPLEFRPERFLTSNADVDLKGQHYELLPFGIGRRM 1481
            +HV  GTRLLVN+WK+ RD NV+  DP EFRPERFLT NAD+D+ GQH+ELLPFG GRR+
Sbjct: 403  FHVKGGTRLLVNVWKLQRDPNVWV-DPTEFRPERFLTENADIDVGGQHFELLPFGAGRRV 461

Query: 1482 CPAVSMAVQLLHLILARVIHEFEINT--QGKVDMREN-MGFLLYKKTPLEVLIRPRI 1643
            CP V  A+Q +HL+LAR+IH +++NT  +  VD+ E+  G + +K +PL++++ PR+
Sbjct: 462  CPGVXFALQFMHLVLARLIHGYDLNTLNEENVDLTESPEGHVNHKASPLDLILTPRL 518


>gb|AFB74614.1| cytochrome P450 [Papaver somniferum] gi|388571246|gb|AFK73719.1|
            cytochrome P450 [Papaver somniferum]
          Length = 540

 Score =  470 bits (1209), Expect = e-130
 Identities = 256/564 (45%), Positives = 360/564 (63%), Gaps = 28/564 (4%)
 Frame = +3

Query: 33   MELFSRL---QSQPISLLIAPLLLGIIVLCCEYGRKSKKTQKQEAPEAXXXXXXXXXXXX 203
            MELF +L   Q  P S+++   +  +++    +    KK ++++AP A            
Sbjct: 1    MELFIKLPFIQPIPFSIILVTTVSIVLLYSVFFWVTDKKKKRKKAPNAAGAW-------- 52

Query: 204  XXXXXXXXXXXXXGRPIMGHLHLF-KAMDSMHQKLGSMADEYGPAFTVRLGSHKTLVVSN 380
                           P++GHL L     + +++ LGSMAD+YGPAF +RLG+ + LVVSN
Sbjct: 53   ---------------PLIGHLRLLMNDKEPLYRALGSMADKYGPAFNIRLGNQEVLVVSN 97

Query: 381  WEMVKECF-TTNDIFFSNHPSILSGKLLFYDVVSVGYAPYGAYWRELRKFSALKLLSSYR 557
            WEMVK+CF   ND  FSN  + L+ K +     S G+APYG YWRELRK    +LLS   
Sbjct: 98   WEMVKQCFGNQNDKLFSNRQTTLAAKYMLNQTTSSGFAPYGPYWRELRKIMVQQLLSKQS 157

Query: 558  LETFKHLRTSEIESSFTFLYNQCKGDNLQFAS-VRLDSWLGDLAFNVITRIVIG-----K 719
            LE++KHL+  E+++SF+ L   C  +    A+ +R+D W  +L FNVI R V G     +
Sbjct: 158  LESWKHLKIKEMDASFSKLNELCNNNGTGTATLIRMDEWFAELTFNVIARNVFGYQSGGR 217

Query: 720  KNFATNG-----AERYKAAMQEAMRLVTVFAFADVFPALSWLDNFTGLTRDLKKCAHEID 884
                TNG      ERYK  ++EA+ L+++FA +D+FP+L W+D   GL R++K+   E++
Sbjct: 218  STALTNGDTESKGERYKKTLEEALHLMSIFAVSDIFPSLEWVDRLRGLIRNMKRFGDELN 277

Query: 885  KVLSGWVEEHRSKRIGG----NKSLELEQDFIDSCLDNLQENSPLPGDDPDIVIKSTCLD 1052
             +    +EEHR KR+      +K +  EQDF+D  L ++ E S LPGDDPD+VIKS  L+
Sbjct: 278  SIAGCLIEEHRQKRLQSVSKSDKGVGDEQDFVDVLL-SVAEKSQLPGDDPDLVIKSMILE 336

Query: 1053 MIMGGSDTLSVTLTWAISLLLNHPDALKRAQEELDTHVGKDRKVDESDLRNFTYIPAIIK 1232
            ++ GGS+T S TLTWA+ LLLNHP  LK+A+EELDTHVGKDR V+ESD     YI AIIK
Sbjct: 337  IVSGGSETTSSTLTWALCLLLNHPHVLKKAKEELDTHVGKDRHVEESDTPKLVYINAIIK 396

Query: 1233 ETMRLYPVGA-VSRSTVEDCEVGGYHVPAGTRLLVNLWKMHRDGNVYKDDPLEFRPERFL 1409
            E+MRLYP GA + R  +E+CEVGG+HVPAG RL VN+WK+ RD +V+ ++PLEF+PER+ 
Sbjct: 397  ESMRLYPNGAMLDRLALEECEVGGFHVPAGGRLFVNVWKIQRDPSVW-ENPLEFKPERWF 455

Query: 1410 TSNA---DVDLKGQHYELLPFGIGRRMCPAVSMAVQLLHLILARVIHEFEI---NTQGKV 1571
             SN    DVD KG ++E +PFGIGRRMC  +  A +++HL+L R+IH F++   +  GKV
Sbjct: 456  LSNGEKMDVDYKGHNHEFIPFGIGRRMCAGMLWASEVIHLVLPRLIHGFDMKAASANGKV 515

Query: 1572 DMRENMGFLL-YKKTPLEVLIRPR 1640
            DM E  G ++ +KKTPLEV++ PR
Sbjct: 516  DMAEMAGMVICFKKTPLEVMVNPR 539


>dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 534

 Score =  464 bits (1194), Expect = e-128
 Identities = 249/540 (46%), Positives = 336/540 (62%), Gaps = 16/540 (2%)
 Frame = +3

Query: 69   SLLIAPLLLGIIVLCCEY---GRKSKKTQKQEAPEAXXXXXXXXXXXXXXXXXXXXXXXX 239
            S L AP+++  IV+   Y    R S   + ++APE                         
Sbjct: 13   STLPAPIIVSFIVILLFYFFKKRSSNMIRTKKAPEVVGAW-------------------- 52

Query: 240  XGRPIMGHLHLFKAMDSMHQKLGSMADEYGPAFTVRLGSHKTLVVSNWEMVKECFTTNDI 419
               P++GHL+L       +  LG +AD+YGPAF+++ G H  LVVS+WE+VK CFTTND 
Sbjct: 53   ---PVIGHLNLLSVPKPAYIVLGELADQYGPAFSIQFGVHPILVVSSWELVKACFTTNDK 109

Query: 420  FFSNHPSILSGKLLFYDVVSVGYAPYGAYWRELRKFSALKLLSSYRLETFKHLRTSEIES 599
            FFS+     + K +FYD  ++ +AP+G YWRELRK   L LLS+ RL+  KH R SE+++
Sbjct: 110  FFSSRLVNKAIKYMFYDQKTISFAPHGPYWRELRKMITLNLLSNERLKMLKHQRISEMDA 169

Query: 600  SFTFLYNQCKGDNLQFASVRLD--SWLGDLAFNVITRIVIGKKNFATNGAERYKAAMQEA 773
                LY        + A V +D   W GD++FNV+TRIV GK  F     ERY   M+EA
Sbjct: 170  CLKKLYELSTKRKDENAGVLVDMSKWFGDISFNVVTRIVAGKHIFGPK-TERYMNVMEEA 228

Query: 774  MRLVTVFAFADVFPALSWLDNFTGLTRDLKKCAHEIDKVLSGWVEEHRSKR------IGG 935
             RL+ V  F+DV P L WLD   G+  ++K+ A E+D  L  WV+EHR KR      IGG
Sbjct: 229  RRLMDVMVFSDVIPYLGWLDRLRGVDSEIKRTAKELDSALESWVDEHRQKRVSISAGIGG 288

Query: 936  --NKSLELEQDFIDSCLDNLQENSPLPGDDPDIVIKSTCLDMIMGGSDTLSVTLTWAISL 1109
              N + E E DFID  L  + +N  L GDDP  +IK+   +M +   D  +VTLTWA+ L
Sbjct: 289  IVNITEEEEIDFIDIMLSIIAKNKLL-GDDPGTLIKAIVQEMYLAAWDNTTVTLTWALCL 347

Query: 1110 LLNHPDALKRAQEELDTHVGKDRKVDESDLRNFTYIPAIIKETMRLYPVGAV-SRSTVED 1286
            LLN+   LKRAQ ELD  VGK+R+V++SD+    YI AI+KE+MRLYP G +  R T ED
Sbjct: 348  LLNNKQVLKRAQCELDAQVGKERQVEDSDINTLPYIQAIVKESMRLYPPGPIIERETTED 407

Query: 1287 CEVGGYHVPAGTRLLVNLWKMHRDGNVYKDDPLEFRPERFLTSNADVDLKGQHYELLPFG 1466
            C+VG + +PAGTRL +NLWK+ RD NV+ +DP EF+PERFL  +AD+D+KGQH+EL+PFG
Sbjct: 408  CDVGDFRIPAGTRLWINLWKLQRDPNVWPNDPQEFQPERFLNGHADIDMKGQHFELIPFG 467

Query: 1467 IGRRMCPAVSMAVQLLHLILARVIHEFEIN--TQGKVDMRENMGFLLYKKTPLEVLIRPR 1640
             GRRMCP VS ++Q++HL+LAR+IH FE+   T   +DM   +G + +K TPLEVL+ PR
Sbjct: 468  SGRRMCPGVSFSLQVMHLVLARIIHGFELKTPTDADIDMSTTLGMISWKATPLEVLLTPR 527