BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver22_contig00009694
         (1306 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName...   719   0.0  
gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]          614   e-173
gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]          595   e-168
pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a ...   574   e-161
pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Comp...   574   e-161

>sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName: Full=Berberine
            bridge-forming enzyme; Short=BBE; AltName:
            Full=Tetrahydroprotoberberine synthase; Flags: Precursor
            gi|3282517|gb|AAC61839.1| berberine bridge enzyme
            [Papaver somniferum]
          Length = 535

 Score =  719 bits (1855), Expect = 0.0
 Identities = 358/414 (86%), Positives = 373/414 (90%)
 Frame = +2

Query: 2    VIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            VIVDMMNL+ ISID  SETAWVESGATLGELYYAIAQST+TLGFTAGWCPTV        
Sbjct: 122  VIVDMMNLNRISIDVLSETAWVESGATLGELYYAIAQSTDTLGFTAGWCPTVGSGGHISG 181

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILIDS GAILDREKMG+DVFWAIR             KIK
Sbjct: 182  GGFGMMSRKYGLAADNVVDAILIDSNGAILDREKMGDDVFWAIRGGGGGVWGAIYAWKIK 241

Query: 362  LLPVPEKLTVFRVTKNVEIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEKLTVFRVTKNV IE ASSLLHKWQYVADELD DFTVSVLGGVNGN+AWLMFLGL
Sbjct: 242  LLPVPEKLTVFRVTKNVGIEDASSLLHKWQYVADELDEDFTVSVLGGVNGNDAWLMFLGL 301

Query: 542  HLGRKDAAKSIIDEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            HLGRKDAAK+IIDEKFPELGLV+KEFQEMSWG SMAFL+GL+TISELNNRFLKFD+RAFK
Sbjct: 302  HLGRKDAAKTIIDEKFPELGLVDKEFQEMSWGESMAFLSGLDTISELNNRFLKFDERAFK 361

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVDFTK +VPL+VFR ALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE
Sbjct: 362  TKVDFTKVSVPLNVFRHALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 421

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YIIAWNQDEE+KIGEFSEWL KFYDYLEPFVSKEPRVGYVNHIDLDIGGIDW+NKSST+N
Sbjct: 422  YIIAWNQDEESKIGEFSEWLAKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWRNKSSTTN 481

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEENYILNEL 1243
            AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEE Y+L EL
Sbjct: 482  AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFEEIYMLKEL 535


>gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]
          Length = 554

 Score =  614 bits (1584), Expect = e-173
 Identities = 292/405 (72%), Positives = 339/405 (83%)
 Frame = +2

Query: 2    VIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            V++D+MNL+ ISID +SETAWVESGATLGELYYAI + T++LGFTAGWCPTV        
Sbjct: 130  VLIDLMNLNRISIDIDSETAWVESGATLGELYYAITELTDSLGFTAGWCPTVGSGGHISG 189

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNV D ILIDSKGAILDR+ MGEDVFWA+R             KIK
Sbjct: 190  GGFGMMSRKYGLAADNVEDVILIDSKGAILDRKLMGEDVFWAVRGGGGGVWGAIYAWKIK 249

Query: 362  LLPVPEKLTVFRVTKNVEIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVP+K+TVFRVTKNV IE AS L+HKWQYVADELD+DFTVS+LGG NGNE W++FLGL
Sbjct: 250  LLPVPKKVTVFRVTKNVNIEEASFLIHKWQYVADELDDDFTVSILGGANGNEVWVIFLGL 309

Query: 542  HLGRKDAAKSIIDEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            HLG K  AKSIID+KFPELGL+E+EF EM+WG S A+L+GL+T+ ELNNRFLKFDDRAFK
Sbjct: 310  HLGCKTVAKSIIDKKFPELGLIEEEFLEMNWGESFAYLSGLKTVKELNNRFLKFDDRAFK 369

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVDFTKE +PL+     LE+LS++P GFIALNGFGGKMS+IS DFTPFPHRKGTKLM E
Sbjct: 370  TKVDFTKETLPLEAIDGLLEILSKEPRGFIALNGFGGKMSKISNDFTPFPHRKGTKLMVE 429

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AW++DEE+K  EF +WL   YDY+E FVSK PRVGYVNHIDLD+GGIDW +K+S++N
Sbjct: 430  YIVAWSKDEESKSDEFFDWLRNIYDYMEMFVSKNPRVGYVNHIDLDLGGIDWSDKNSSNN 489

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKF 1216
            A+EIARNWGE+YF SNYERL++AKTLIDPNNVFNHPQSIPPMMK+
Sbjct: 490  AIEIARNWGEKYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMMKY 534


>gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]
          Length = 536

 Score =  595 bits (1534), Expect = e-168
 Identities = 281/406 (69%), Positives = 333/406 (82%)
 Frame = +2

Query: 2    VIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            V++D+MNL+ ISID +SETAWVESGAT+GELYYAI++ST++ GFTAGWCPTV        
Sbjct: 125  VLIDLMNLNRISIDMDSETAWVESGATVGELYYAISESTDSFGFTAGWCPTVGTGGHISG 184

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNV D ILIDS GAILDR+ MGEDVFWA+R             KIK
Sbjct: 185  GGFGMMSRKYGLAADNVEDVILIDSNGAILDRKLMGEDVFWAVRGGGGGVWGAIYAWKIK 244

Query: 362  LLPVPEKLTVFRVTKNVEIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVP+K+TVFRV KNV IE AS L+HKWQYVADELD+DFTV++LGG NGN AWL+FLGL
Sbjct: 245  LLPVPKKVTVFRVMKNVNIEEASFLIHKWQYVADELDDDFTVTILGGANGNGAWLVFLGL 304

Query: 542  HLGRKDAAKSIIDEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            HLG K  AKSI+D+ FPELGL+E+EF EM+WG S A+L+GL+T+ ELNNRFLK DD+AFK
Sbjct: 305  HLGCKTVAKSIMDKMFPELGLIEEEFLEMNWGESFAYLSGLKTVKELNNRFLKLDDKAFK 364

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVDFTKE +PL V    LE+LS++P GFI LN  GGKMS+IS DFTPFPHR GTKLM E
Sbjct: 365  TKVDFTKETLPLKVINGVLEILSKEPRGFILLNSLGGKMSKISNDFTPFPHRNGTKLMVE 424

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI++W++DEE+K  EF +WL   YDY+E FVSK PRVGYVN+IDLD+GGIDW NK+S++N
Sbjct: 425  YIVSWSKDEESKSDEFFDWLRNIYDYMEEFVSKNPRVGYVNNIDLDLGGIDWSNKNSSNN 484

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFE 1219
            A+EIARNWGE+YF SNYERL++AKTLIDPNN+FNHPQSIPPMMKF+
Sbjct: 485  AIEIARNWGEKYFLSNYERLIRAKTLIDPNNIFNHPQSIPPMMKFD 530


>pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In
            Complex With (S)-Reticuline
          Length = 519

 Score =  574 bits (1480), Expect = e-161
 Identities = 273/406 (67%), Positives = 324/406 (79%)
 Frame = +2

Query: 2    VIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            +++D+MNL+ +SID ESETAWVESG+TLGELYYAI +S+  LGFTAGWCPTV        
Sbjct: 99   ILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGAISG 158

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR             KIK
Sbjct: 159  GGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIK 218

Query: 362  LLPVPEKLTVFRVTKNVEIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SVLGG +  + WL  LG 
Sbjct: 219  LLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSVLGGADEKQVWLTMLGF 278

Query: 542  HLGRKDAAKSIIDEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            H G K  AKS  D  FPELGLVE+++ EMSWG S A+LAGLET+S+LNNRFLKFD+RAFK
Sbjct: 279  HFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETVSQLNNRFLKFDERAFK 338

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR GT+LM E
Sbjct: 339  TKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVE 398

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW NK+  +N
Sbjct: 399  YIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNN 458

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFE 1219
            A+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM  F+
Sbjct: 459  AIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMANFD 504


>pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
            Dehydroscoulerine
          Length = 519

 Score =  574 bits (1480), Expect = e-161
 Identities = 273/406 (67%), Positives = 324/406 (79%)
 Frame = +2

Query: 2    VIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXX 181
            +++D+MNL+ +SID ESETAWVESG+TLGELYYAI +S+  LGFTAGWCPTV        
Sbjct: 99   ILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISG 158

Query: 182  XXXXXXXRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXXXXXXXXXXKIK 361
                   RKYGLAADNVVDAILID+ GAILDR+ MGEDVFWAIR             KIK
Sbjct: 159  GGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIK 218

Query: 362  LLPVPEKLTVFRVTKNVEIEAASSLLHKWQYVADELDNDFTVSVLGGVNGNEAWLMFLGL 541
            LLPVPEK+TVFRVTKNV I+ A+SLLHKWQ+VA+EL+ DFT+SVLGG +  + WL  LG 
Sbjct: 219  LLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSVLGGADEKQVWLTMLGF 278

Query: 542  HLGRKDAAKSIIDEKFPELGLVEKEFQEMSWGASMAFLAGLETISELNNRFLKFDDRAFK 721
            H G K  AKS  D  FPELGLVE+++ EMSWG S A+LAGLET+S+LNNRFLKFD+RAFK
Sbjct: 279  HFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETVSQLNNRFLKFDERAFK 338

Query: 722  TKVDFTKEAVPLDVFRRALEMLSEQPGGFIALNGFGGKMSEISTDFTPFPHRKGTKLMFE 901
            TKVD TKE +P   F   LE LS++P GFIALNGFGG+MS+IS+DFTPFPHR GT+LM E
Sbjct: 339  TKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVE 398

Query: 902  YIIAWNQDEETKIGEFSEWLEKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWKNKSSTSN 1081
            YI+AWNQ E+ K  EF +WLEK Y++++PFVSK PR+GYVNHIDLD+GGIDW NK+  +N
Sbjct: 399  YIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNN 458

Query: 1082 AVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPMMKFE 1219
            A+EI+R+WGE YF SNYERL++AKTLIDPNNVFNHPQSIPPM  F+
Sbjct: 459  AIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMANFD 504