BLASTX nr result
ID: Papaver22_contig00005165
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver22_contig00005165
(1543 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi... 500 0.0
sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin... 492 0.0
sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydropro... 445 e-158
sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin... 410 e-144
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;... 271 8e-89
>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 gi|226897732|gb|ACO90237.1|
tetrahydroprotoberberine N-methyltransferase [Papaver
bracteatum]
Length = 358
Score = 500 bits (1288), Expect(2) = 0.0
Identities = 242/242 (100%), Positives = 242/242 (100%)
Frame = +3
Query: 486 HELYCERAQIKDGQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLG 665
HELYCERAQIKDGQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLG
Sbjct: 117 HELYCERAQIKDGQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLG 176
Query: 666 LTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEESLLFVDHVCHK 845
LTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEESLLFVDHVCHK
Sbjct: 177 LTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEESLLFVDHVCHK 236
Query: 846 TFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIW 1025
TFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIW
Sbjct: 237 TFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIW 296
Query: 1026 RKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFK 1205
RKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFK
Sbjct: 297 RKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFK 356
Query: 1206 KK 1211
KK
Sbjct: 357 KK 358
Score = 185 bits (469), Expect(2) = 0.0
Identities = 94/110 (85%), Positives = 94/110 (85%)
Frame = +2
Query: 53 MGSIDEVKKESAGETLGRXXXXXXXXXXXXXXXXFQFEKRLQWGYKSSHQEQLSFNLDFI 232
MGSIDEVKKESAGETLGR FQFEKRLQWGYKSSHQEQLSFNLDFI
Sbjct: 1 MGSIDEVKKESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFI 60
Query: 233 KSLKKMEMSGEIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATID 382
KSLKKMEMSGEIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATID
Sbjct: 61 KSLKKMEMSGEIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATID 110
>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT gi|67764091|gb|AAY79177.1|
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-cis-
N-methyltransferase [Papaver somniferum]
Length = 358
Score = 492 bits (1266), Expect(2) = 0.0
Identities = 237/242 (97%), Positives = 240/242 (99%)
Frame = +3
Query: 486 HELYCERAQIKDGQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLG 665
HELYCERAQIKDGQTVLDIGCGQGGLVLYIA+KYK CHVTGLTNSKAQVNYLLKQAEKLG
Sbjct: 117 HELYCERAQIKDGQTVLDIGCGQGGLVLYIAQKYKNCHVTGLTNSKAQVNYLLKQAEKLG 176
Query: 666 LTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEESLLFVDHVCHK 845
LTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMT+ESLLFVDHVCHK
Sbjct: 177 LTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTKESLLFVDHVCHK 236
Query: 846 TFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIW 1025
TFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIW
Sbjct: 237 TFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIW 296
Query: 1026 RKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFK 1205
RKALDKNMEAAKEILLPGLGGSHE VNGVVTHIRTFCMGGYEQFSMN+GDEWMVAQLLFK
Sbjct: 297 RKALDKNMEAAKEILLPGLGGSHETVNGVVTHIRTFCMGGYEQFSMNNGDEWMVAQLLFK 356
Query: 1206 KK 1211
KK
Sbjct: 357 KK 358
Score = 182 bits (463), Expect(2) = 0.0
Identities = 93/110 (84%), Positives = 93/110 (84%)
Frame = +2
Query: 53 MGSIDEVKKESAGETLGRXXXXXXXXXXXXXXXXFQFEKRLQWGYKSSHQEQLSFNLDFI 232
MGSIDEVKKESAGETLGR FQFEKRLQWGYKSSHQEQLSFNLDFI
Sbjct: 1 MGSIDEVKKESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFI 60
Query: 233 KSLKKMEMSGEIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATID 382
KSLKKMEMSGEIETMNKETYELPSEFLEAVFGKTVKQSMCYF HESATID
Sbjct: 61 KSLKKMEMSGEIETMNKETYELPSEFLEAVFGKTVKQSMCYFTHESATID 110
>sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2
gi|226897730|gb|ACO90236.1| putative N-methyltransferase
[Papaver bracteatum]
Length = 358
Score = 445 bits (1144), Expect(2) = e-158
Identities = 207/242 (85%), Positives = 225/242 (92%)
Frame = +3
Query: 486 HELYCERAQIKDGQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLG 665
HELYCERAQIKDGQTVLDIGCGQGGLVLY+A+KYK CHVTGLTNSK QVNY+LKQAEKLG
Sbjct: 117 HELYCERAQIKDGQTVLDIGCGQGGLVLYVAQKYKNCHVTGLTNSKEQVNYILKQAEKLG 176
Query: 666 LTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEESLLFVDHVCHK 845
L NVD ILADVTQYESDKTYDR+L+I +EHMKN+QLF+KKLSTWM E+SLLFVDH CHK
Sbjct: 177 LRNVDVILADVTQYESDKTYDRILVIGVVEHMKNMQLFIKKLSTWMAEDSLLFVDHSCHK 236
Query: 846 TFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIW 1025
TF HFFEA+DEDDWYSG+IFPPGCAT L+A+SLLYFQDDVSVVDHWVVNGMH AR+VD W
Sbjct: 237 TFNHFFEALDEDDWYSGYIFPPGCATFLSADSLLYFQDDVSVVDHWVVNGMHFARTVDAW 296
Query: 1026 RKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFK 1205
RK LDKNMEA KEILLPGLGG+HEAVNGV+THIRT C+GGY QFS+NDGDEWM AQLLFK
Sbjct: 297 RKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDGDEWMNAQLLFK 356
Query: 1206 KK 1211
KK
Sbjct: 357 KK 358
Score = 142 bits (359), Expect(2) = e-158
Identities = 71/110 (64%), Positives = 80/110 (72%)
Frame = +2
Query: 53 MGSIDEVKKESAGETLGRXXXXXXXXXXXXXXXXFQFEKRLQWGYKSSHQEQLSFNLDFI 232
MGSI+EVKKESA ETLGR +Q EKRLQWGYKSSHQEQLSFNLDFI
Sbjct: 1 MGSIEEVKKESAEETLGRLLRGEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFI 60
Query: 233 KSLKKMEMSGEIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATID 382
SLKKM MSG++E E YELP+E EA +GK++K S CYFKHES+TID
Sbjct: 61 NSLKKMGMSGQVEAFTNEVYELPTECFEAAYGKSMKLSGCYFKHESSTID 110
>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT gi|226897702|gb|ACO90222.1|
tetrahydroprotoberberine N-methyltransferase
[Eschscholzia californica]
Length = 350
Score = 410 bits (1053), Expect(2) = e-144
Identities = 191/242 (78%), Positives = 219/242 (90%)
Frame = +3
Query: 486 HELYCERAQIKDGQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLG 665
HELYCERAQIKDGQTVLDIGCGQGGLVLYIA KYK CHVTGLTNSKAQ NY+ +QAEKL
Sbjct: 110 HELYCERAQIKDGQTVLDIGCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLE 169
Query: 666 LTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEESLLFVDHVCHK 845
LTNVD I ADVT++++DKTYDR+L++E IEHMKN+QLFMKKLSTWMTE+SLLFVDH+ HK
Sbjct: 170 LTNVDVIFADVTKFDTDKTYDRILVVETIEHMKNIQLFMKKLSTWMTEDSLLFVDHISHK 229
Query: 846 TFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIW 1025
TF H FEA+DEDDWYSGFIFP GC TIL++++LLYFQDDVS +DHWVVNGMHMARSV+ W
Sbjct: 230 TFNHNFEALDEDDWYSGFIFPKGCVTILSSSTLLYFQDDVSALDHWVVNGMHMARSVEAW 289
Query: 1026 RKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFK 1205
RK LD+ +EAA+EIL PGL GS EAVN V+THIRTFC+GGYEQFS N+G+EWM+ Q+LFK
Sbjct: 290 RKKLDETIEAAREILEPGL-GSKEAVNQVITHIRTFCIGGYEQFSYNNGEEWMITQILFK 348
Query: 1206 KK 1211
KK
Sbjct: 349 KK 350
Score = 129 bits (323), Expect(2) = e-144
Identities = 61/100 (61%), Positives = 76/100 (76%)
Frame = +2
Query: 83 SAGETLGRXXXXXXXXXXXXXXXXFQFEKRLQWGYKSSHQEQLSFNLDFIKSLKKMEMSG 262
SAGE +GR Q+++R++WGYK +H++QL+FNLDFIK LK+M MSG
Sbjct: 4 SAGEIMGRLMKGEIEDEELKKLIRHQWDRRIEWGYKPTHEKQLAFNLDFIKGLKEMVMSG 63
Query: 263 EIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATID 382
EI+TMNKETYELP+ FLEAVFGKTVKQS CYFK E++TID
Sbjct: 64 EIDTMNKETYELPTAFLEAVFGKTVKQSCCYFKDENSTID 103
>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
[Thalictrum flavum]
Length = 356
Score = 271 bits (693), Expect(2) = 8e-89
Identities = 122/241 (50%), Positives = 176/241 (73%)
Frame = +3
Query: 489 ELYCERAQIKDGQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLGL 668
+LYCERAQIKDGQ++LD+GCG G L L++A+KY+ C VTG+TNS +Q +++ Q +KL L
Sbjct: 117 DLYCERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDL 176
Query: 669 TNVDAILADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEESLLFVDHVCHKT 848
+NV+ IL DVT++E++ TYDR+ + IEHMKN +LF+KK+STW+ + LLFV+H CHK
Sbjct: 177 SNVEIILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKV 236
Query: 849 FAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWR 1028
FA+ +E +DEDDWY+ +IFP G + +++ LLYFQ+DVSVV+HW ++G H + W
Sbjct: 237 FAYQYEPLDEDDWYTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWL 296
Query: 1029 KALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFKK 1208
K LD N++ KEI GS E +T+ R FC+ + +S N+G+EWM++Q+LFKK
Sbjct: 297 KRLDDNIDEVKEI-FESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQVLFKK 355
Query: 1209 K 1211
K
Sbjct: 356 K 356
Score = 84.3 bits (207), Expect(2) = 8e-89
Identities = 42/105 (40%), Positives = 62/105 (59%)
Frame = +2
Query: 68 EVKKESAGETLGRXXXXXXXXXXXXXXXXFQFEKRLQWGYKSSHQEQLSFNLDFIKSLKK 247
+ KKE+ + R Q +KRL+WGYK +H++QL+ + F +SLK
Sbjct: 5 QTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKG 64
Query: 248 MEMSGEIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATID 382
MEM+ E++T++ E YE+P FL + GKT+K S YFK ES T+D
Sbjct: 65 MEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLD 109