BLASTX nr result
ID: Panax25_contig00018515
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Panax25_contig00018515
(466 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_019072638.1 PREDICTED: DNA polymerase delta subunit 3 isoform... 86 1e-16
XP_019072637.1 PREDICTED: DNA polymerase delta subunit 3 isoform... 86 1e-16
CBI20169.3 unnamed protein product, partial [Vitis vinifera] 86 1e-16
XP_002283370.1 PREDICTED: DNA polymerase delta subunit 3 isoform... 86 1e-16
XP_017243932.1 PREDICTED: DNA polymerase delta subunit 3 [Daucus... 75 7e-13
XP_011460802.1 PREDICTED: uncharacterized protein LOC105350465 [... 70 5e-11
XP_007213902.1 hypothetical protein PRUPE_ppa003898mg [Prunus pe... 69 9e-11
XP_018839098.1 PREDICTED: nucleolar and coiled-body phosphoprote... 67 3e-10
XP_008226385.1 PREDICTED: DNA polymerase delta subunit 3 [Prunus... 60 8e-08
XP_011082780.1 PREDICTED: uncharacterized protein LOC105165459 i... 59 4e-07
XP_011082779.1 PREDICTED: uncharacterized protein LOC105165459 i... 59 4e-07
CDP06031.1 unnamed protein product [Coffea canephora] 58 5e-07
GAV60698.1 CDC27 domain-containing protein [Cephalotus follicula... 58 7e-07
XP_012455548.1 PREDICTED: uncharacterized protein LOC105777044 [... 58 7e-07
OMO74704.1 Peptidase C26 [Corchorus capsularis] 58 7e-07
OMO92528.1 DNA polymerase subunit Cdc27 [Corchorus olitorius] 57 9e-07
XP_016545688.1 PREDICTED: nucleolin 1 isoform X2 [Capsicum annuum] 57 1e-06
XP_016545687.1 PREDICTED: nucleolin 1 isoform X1 [Capsicum annuum] 57 1e-06
XP_016722664.1 PREDICTED: uncharacterized protein LOC107934693 [... 57 2e-06
XP_012846796.1 PREDICTED: uncharacterized protein LOC105966761 [... 56 2e-06
>XP_019072638.1 PREDICTED: DNA polymerase delta subunit 3 isoform X3 [Vitis
vinifera]
Length = 467
Score = 85.9 bits (211), Expect = 1e-16
Identities = 55/135 (40%), Positives = 65/135 (48%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D D NFKRAS G+ RKRRVVFDFSDEE+E++D V Q K V
Sbjct: 294 DGQDANFKRASNGDGGRKRRVVFDFSDEEEEFEDAVNLASPDPPKGKSCIVSKQSPKPLV 353
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
+KI+LN + ESN RE+S +SKGKNNGI E
Sbjct: 354 PDKINLNSDQQKQDKPKVKEEKSSNRESNLSPREDSSVLSKGKNNGISSSDKIAGGVPEI 413
Query: 363 DACTKDKLTGAALKS 407
D KDK+T AA S
Sbjct: 414 DVNKKDKVTDAAPNS 428
>XP_019072637.1 PREDICTED: DNA polymerase delta subunit 3 isoform X2 [Vitis
vinifera]
Length = 468
Score = 85.9 bits (211), Expect = 1e-16
Identities = 55/135 (40%), Positives = 65/135 (48%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D D NFKRAS G+ RKRRVVFDFSDEE+E++D V Q K V
Sbjct: 294 DGQDANFKRASNGDGGRKRRVVFDFSDEEEEFEDAVNLASPDPPKGKSCIVSKQSPKPLV 353
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
+KI+LN + ESN RE+S +SKGKNNGI E
Sbjct: 354 PDKINLNSDQQKQDKPKVKEEKSSNRESNLSPREDSSVLSKGKNNGISSSDKIAGGVPEI 413
Query: 363 DACTKDKLTGAALKS 407
D KDK+T AA S
Sbjct: 414 DVNKKDKVTDAAPNS 428
>CBI20169.3 unnamed protein product, partial [Vitis vinifera]
Length = 477
Score = 85.9 bits (211), Expect = 1e-16
Identities = 55/135 (40%), Positives = 65/135 (48%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D D NFKRAS G+ RKRRVVFDFSDEE+E++D V Q K V
Sbjct: 247 DGQDANFKRASNGDGGRKRRVVFDFSDEEEEFEDAVNLASPDPPKGKSCIVSKQSPKPLV 306
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
+KI+LN + ESN RE+S +SKGKNNGI E
Sbjct: 307 PDKINLNSDQQKQDKPKVKEEKSSNRESNLSPREDSSVLSKGKNNGISSSDKIAGGVPEI 366
Query: 363 DACTKDKLTGAALKS 407
D KDK+T AA S
Sbjct: 367 DVNKKDKVTDAAPNS 381
>XP_002283370.1 PREDICTED: DNA polymerase delta subunit 3 isoform X1 [Vitis
vinifera]
Length = 524
Score = 85.9 bits (211), Expect = 1e-16
Identities = 55/135 (40%), Positives = 65/135 (48%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D D NFKRAS G+ RKRRVVFDFSDEE+E++D V Q K V
Sbjct: 294 DGQDANFKRASNGDGGRKRRVVFDFSDEEEEFEDAVNLASPDPPKGKSCIVSKQSPKPLV 353
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
+KI+LN + ESN RE+S +SKGKNNGI E
Sbjct: 354 PDKINLNSDQQKQDKPKVKEEKSSNRESNLSPREDSSVLSKGKNNGISSSDKIAGGVPEI 413
Query: 363 DACTKDKLTGAALKS 407
D KDK+T AA S
Sbjct: 414 DVNKKDKVTDAAPNS 428
>XP_017243932.1 PREDICTED: DNA polymerase delta subunit 3 [Daucus carota subsp.
sativus] KZM99369.1 hypothetical protein DCAR_013269
[Daucus carota subsp. sativus]
Length = 568
Score = 75.1 bits (183), Expect = 7e-13
Identities = 51/138 (36%), Positives = 66/138 (47%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
DD N KR+S GE +RKRRV FD SDEED++K+ V CSKA
Sbjct: 298 DDLGINIKRSSNGEGSRKRRVFFDSSDEEDDFKEAVSLASPGSPKLKSSSNLDLCSKAHS 357
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
EK SL+ ++ ++ L R+ES+ VSK NGI T
Sbjct: 358 GEKSSLDPKEHKEKKMEVKEENNMKKAADPLPRKESV-VSKNNKNGIHASDKVVSHNPGT 416
Query: 363 DACTKDKLTGAALKSSSI 416
+AC+KD T A+L SS I
Sbjct: 417 NACSKDNGTSASLNSSRI 434
>XP_011460802.1 PREDICTED: uncharacterized protein LOC105350465 [Fragaria vesca
subsp. vesca]
Length = 585
Score = 69.7 bits (169), Expect = 5e-11
Identities = 49/132 (37%), Positives = 59/132 (44%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
DD D NFKRASI E RKRRVVFDFSD EDE +D V +CS+ V
Sbjct: 302 DDQDVNFKRASISEVTRKRRVVFDFSD-EDECEDAVNLASPECPKGKSYQEDKECSETLV 360
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
K +L F+ + ESN RE+S VSK K + I +
Sbjct: 361 PHKSNLKFDEQAEDKPEVKEEISVDGESNPPCREDSTVVSKDKISPIILTEKTDRFGPQN 420
Query: 363 DACTKDKLTGAA 398
+ DKL AA
Sbjct: 421 NPNKMDKLATAA 432
>XP_007213902.1 hypothetical protein PRUPE_ppa003898mg [Prunus persica] ONI12372.1
hypothetical protein PRUPE_4G160000 [Prunus persica]
Length = 541
Score = 68.9 bits (167), Expect = 9e-11
Identities = 54/149 (36%), Positives = 64/149 (42%), Gaps = 14/149 (9%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D H+ NFKRAS GE RKRRVVFDFSD +DE +D V + SK V
Sbjct: 303 DGHEVNFKRASNGEGTRKRRVVFDFSD-DDEDEDAVNLASPDNQKEQSCQDLKESSKVFV 361
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIR------------TESNQLLREES--LDVSKGKNNG 320
E SLNF+ + +SNQ RE+S +SKG+N G
Sbjct: 362 PEGTSLNFDEQVEDKPKVEDKPKVEDKPMVKEEVSVDRKSNQSFREDSSVSGISKGRNAG 421
Query: 321 IXXXXXXXXXXXETDACTKDKLTGAALKS 407
I E D KDKL AA S
Sbjct: 422 IILKEKTHSCIPEKDLNKKDKLNTAASSS 450
>XP_018839098.1 PREDICTED: nucleolar and coiled-body phosphoprotein 1 [Juglans
regia]
Length = 564
Score = 67.4 bits (163), Expect = 3e-10
Identities = 48/135 (35%), Positives = 57/135 (42%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D D NFKRAS E RKRRVVFDFSDE++E +D V Q +K
Sbjct: 333 DGQDVNFKRASNAEGIRKRRVVFDFSDEDEENEDAVNLASPDIPKVRSSLDQKQSTKILA 392
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
EK +LNF+ ESNQ +RE SL SK G E+
Sbjct: 393 SEKSNLNFDKEIEDKPMVKEGIATNKESNQPVRENSLVTSKATEAGTSSKERVQSGIPES 452
Query: 363 DACTKDKLTGAALKS 407
D K+ T A S
Sbjct: 453 DINRKNTPTLAVPNS 467
>XP_008226385.1 PREDICTED: DNA polymerase delta subunit 3 [Prunus mume]
Length = 541
Score = 60.5 bits (145), Expect = 8e-08
Identities = 52/149 (34%), Positives = 60/149 (40%), Gaps = 14/149 (9%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D H+ NFKRAS GE RKRRVVFDFSD +DE +D V + SK V
Sbjct: 303 DGHEVNFKRASNGEGTRKRRVVFDFSD-DDEDEDAVNLASPDNPKAQSCQNPKESSKVLV 361
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIR------------TESNQLLREES--LDVSKGKNNG 320
E SLNF + +SNQ RE+S VSKG+N G
Sbjct: 362 PEGNSLNFEEQVEDKPKVEDKPKVEDKPMVKEEVSVDRKSNQSFREDSSVSGVSKGRNAG 421
Query: 321 IXXXXXXXXXXXETDACTKDKLTGAALKS 407
I E DK AA S
Sbjct: 422 IILKEKMHSCIPEKYLNKMDKPNNAASSS 450
>XP_011082780.1 PREDICTED: uncharacterized protein LOC105165459 isoform X2 [Sesamum
indicum]
Length = 517
Score = 58.5 bits (140), Expect = 4e-07
Identities = 46/142 (32%), Positives = 58/142 (40%), Gaps = 3/142 (2%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKA-- 176
D DFN KR S GE RKRRVVFD+SDEEDEY+D + CSK
Sbjct: 294 DGQDFNIKRTSNGEGGRKRRVVFDYSDEEDEYRDAISLASPDPPKKSAL-----CSKGRS 348
Query: 177 -SVVEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXX 353
++ +L+F E+ Q L E+ SK K
Sbjct: 349 DALGPGCNLSFEEKENKPKVNEVKE-AGVEAKQHLGEKVSATSKSKKAESPTSEKNLNHV 407
Query: 354 XETDACTKDKLTGAALKSSSIV 419
ETDA KDK+ A K ++
Sbjct: 408 PETDASLKDKVADAGPKRRKVL 429
>XP_011082779.1 PREDICTED: uncharacterized protein LOC105165459 isoform X1 [Sesamum
indicum]
Length = 529
Score = 58.5 bits (140), Expect = 4e-07
Identities = 46/142 (32%), Positives = 58/142 (40%), Gaps = 3/142 (2%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKA-- 176
D DFN KR S GE RKRRVVFD+SDEEDEY+D + CSK
Sbjct: 306 DGQDFNIKRTSNGEGGRKRRVVFDYSDEEDEYRDAISLASPDPPKKSAL-----CSKGRS 360
Query: 177 -SVVEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXX 353
++ +L+F E+ Q L E+ SK K
Sbjct: 361 DALGPGCNLSFEEKENKPKVNEVKE-AGVEAKQHLGEKVSATSKSKKAESPTSEKNLNHV 419
Query: 354 XETDACTKDKLTGAALKSSSIV 419
ETDA KDK+ A K ++
Sbjct: 420 PETDASLKDKVADAGPKRRKVL 441
>CDP06031.1 unnamed protein product [Coffea canephora]
Length = 513
Score = 58.2 bits (139), Expect = 5e-07
Identities = 42/130 (32%), Positives = 53/130 (40%)
Frame = +3
Query: 18 NFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASVVEKIS 197
N +R+ GE RKRRVVFDFSDEEDE+KD V + S +S +EK
Sbjct: 294 NVRRSYNGEVGRKRRVVFDFSDEEDEFKDAVNLASPDPPKKKSMLDSKRSSYSSDIEKSK 353
Query: 198 LNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXETDACTK 377
LNF R E+++ +EE+L N D K
Sbjct: 354 LNFEEEKEDKPNFKH----RKETDRKSKEENLAFGNHNNMNSSSSEKVINHVPAVDTIAK 409
Query: 378 DKLTGAALKS 407
DK AA S
Sbjct: 410 DKTRDAAPNS 419
>GAV60698.1 CDC27 domain-containing protein [Cephalotus follicularis]
Length = 526
Score = 57.8 bits (138), Expect = 7e-07
Identities = 44/132 (33%), Positives = 58/132 (43%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D + N KRAS GE RKRRVVFD+SDEEDEY V Q ++ +
Sbjct: 297 DGQNANVKRASNGEGGRKRRVVFDYSDEEDEYGGAVNLASPDPPKGKLSPDSKQRTETLL 356
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
+EK +L F+ + E+ Q +EE VSKG + E
Sbjct: 357 LEKKNLIFDKQKEPEPEVKEVKAAKIET-QPPKEELSVVSKGMKPKMASLEKIQRSNLEK 415
Query: 363 DACTKDKLTGAA 398
DA +D +T AA
Sbjct: 416 DANKEDSVTNAA 427
>XP_012455548.1 PREDICTED: uncharacterized protein LOC105777044 [Gossypium
raimondii] KJB13012.1 hypothetical protein
B456_002G051000 [Gossypium raimondii]
Length = 536
Score = 57.8 bits (138), Expect = 7e-07
Identities = 47/135 (34%), Positives = 60/135 (44%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D ++ NF+RAS GE N+KRRVVFDFSD EDE +D V Q SK V
Sbjct: 301 DAYEVNFRRASYGEGNKKRRVVFDFSD-EDENEDAVNLASPDLPKRKSLLGSEQDSKTLV 359
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
E+ + + E N+ LREE+ VSK + NG ET
Sbjct: 360 SERPNSIVDKPNKDEVNIKEETMTDGEPNRSLREETSLVSK-RTNGRNSVVKLESQLPET 418
Query: 363 DACTKDKLTGAALKS 407
D++T AA S
Sbjct: 419 APNKMDEVTDAAPNS 433
>OMO74704.1 Peptidase C26 [Corchorus capsularis]
Length = 898
Score = 57.8 bits (138), Expect = 7e-07
Identities = 47/135 (34%), Positives = 53/135 (39%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D D NF+RAS GE NRKRRVVFDFSD EDEY+D V Q +K
Sbjct: 660 DAQDVNFRRASNGEGNRKRRVVFDFSD-EDEYEDAVNLASPDPPKKKPFLDSEQNAKTLA 718
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
+ L E N L EE VSK N +
Sbjct: 719 TKMPDLIEEKPNKDEVKVKEERTTDGEPNISLVEEISLVSKSTNGRHSSLVKVENQLPDA 778
Query: 363 DACTKDKLTGAALKS 407
D KDK+T AA S
Sbjct: 779 DVSKKDKVTDAAPNS 793
>OMO92528.1 DNA polymerase subunit Cdc27 [Corchorus olitorius]
Length = 553
Score = 57.4 bits (137), Expect = 9e-07
Identities = 46/135 (34%), Positives = 53/135 (39%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D D NF+RAS GE NRKRRVVFDFSD EDEY+D V Q +K
Sbjct: 315 DAQDVNFRRASNGEGNRKRRVVFDFSD-EDEYEDAVNLASPDPPKKKPFLDSEQNAKTLA 373
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
+ L E N L +E VSK N +
Sbjct: 374 TKMPDLTEEKPNKDEVKVKEERTTDREPNISLVKEISLVSKSTNGRHSSLVKVENQLPDA 433
Query: 363 DACTKDKLTGAALKS 407
D KDK+T AA S
Sbjct: 434 DLSKKDKVTDAAPNS 448
>XP_016545688.1 PREDICTED: nucleolin 1 isoform X2 [Capsicum annuum]
Length = 518
Score = 57.0 bits (136), Expect = 1e-06
Identities = 25/36 (69%), Positives = 28/36 (77%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVV 110
DD N +R S GE NRKRRV+FDFSDEEDE+KD V
Sbjct: 282 DDQQVNIRRTSNGEGNRKRRVIFDFSDEEDEFKDAV 317
>XP_016545687.1 PREDICTED: nucleolin 1 isoform X1 [Capsicum annuum]
Length = 519
Score = 57.0 bits (136), Expect = 1e-06
Identities = 25/36 (69%), Positives = 28/36 (77%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVV 110
DD N +R S GE NRKRRV+FDFSDEEDE+KD V
Sbjct: 283 DDQQVNIRRTSNGEGNRKRRVIFDFSDEEDEFKDAV 318
>XP_016722664.1 PREDICTED: uncharacterized protein LOC107934693 [Gossypium
hirsutum]
Length = 535
Score = 56.6 bits (135), Expect = 2e-06
Identities = 46/135 (34%), Positives = 60/135 (44%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVVXXXXXXXXXXXXXXXXXQCSKASV 182
D ++ NF+RAS GE N+KRRVVFDFSD EDE +D V Q SK V
Sbjct: 301 DAYEVNFRRASYGEGNKKRRVVFDFSD-EDENEDAVNLASPDLPKRKSLLGSEQDSKTLV 359
Query: 183 VEKISLNFNXXXXXXXXXXXXXYIRTESNQLLREESLDVSKGKNNGIXXXXXXXXXXXET 362
E+ + + E N+ LREE+ +SK + NG ET
Sbjct: 360 SERPNSIVDKPNKDEVNIKEETMTDGEPNRSLREETSLLSK-RTNGRNSVVKLESQLPET 418
Query: 363 DACTKDKLTGAALKS 407
D++T AA S
Sbjct: 419 APNKMDEVTDAAPNS 433
>XP_012846796.1 PREDICTED: uncharacterized protein LOC105966761 [Erythranthe
guttata]
Length = 520
Score = 56.2 bits (134), Expect = 2e-06
Identities = 25/36 (69%), Positives = 28/36 (77%)
Frame = +3
Query: 3 DDHDFNFKRASIGEANRKRRVVFDFSDEEDEYKDVV 110
D DFN KR S GE RKRRVVFD+SD+EDEY+D V
Sbjct: 285 DGEDFNVKRTSNGEGGRKRRVVFDYSDDEDEYRDAV 320