BLASTX nr result
ID: Panax24_contig00040593
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Panax24_contig00040593
(527 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_017241194.1 PREDICTED: uncharacterized protein LOC108213921 [... 117 1e-30
KZN02701.1 hypothetical protein DCAR_011456 [Daucus carota subsp... 93 6e-22
XP_017221486.1 PREDICTED: uncharacterized protein LOC108198230 [... 95 1e-21
KZN08719.1 hypothetical protein DCAR_001375 [Daucus carota subsp... 85 6e-18
XP_019249558.1 PREDICTED: uncharacterized protein LOC109228806 [... 68 4e-11
XP_009779351.1 PREDICTED: uncharacterized protein LOC104228571 [... 68 4e-11
XP_016551799.1 PREDICTED: uncharacterized protein LOC107851340 [... 65 3e-10
XP_016465961.1 PREDICTED: uncharacterized protein LOC107788766 [... 65 6e-10
XP_009626113.1 PREDICTED: uncharacterized protein LOC104116868 [... 65 6e-10
XP_016483665.1 PREDICTED: uncharacterized protein LOC107804307 [... 63 1e-09
XP_009598905.1 PREDICTED: uncharacterized protein LOC104094647 [... 60 2e-08
XP_009779353.1 PREDICTED: uncharacterized protein LOC104228573 [... 59 2e-08
XP_007048138.2 PREDICTED: uncharacterized protein LOC18611688 [T... 59 7e-08
EOX92295.1 Uncharacterized protein TCM_001261 [Theobroma cacao] 59 9e-08
XP_015071800.1 PREDICTED: uncharacterized protein LOC107015887 [... 59 1e-07
XP_018810759.1 PREDICTED: uncharacterized protein LOC108983545 [... 58 2e-07
XP_019254030.1 PREDICTED: uncharacterized protein LOC109232745 [... 55 2e-06
XP_004237464.1 PREDICTED: uncharacterized protein LOC101257462 [... 54 4e-06
XP_008356960.1 PREDICTED: uncharacterized protein LOC103420692 i... 54 9e-06
>XP_017241194.1 PREDICTED: uncharacterized protein LOC108213921 [Daucus carota
subsp. sativus]
Length = 145
Score = 117 bits (293), Expect = 1e-30
Identities = 68/137 (49%), Positives = 85/137 (62%), Gaps = 4/137 (2%)
Frame = -2
Query: 526 LFIFSLIILFPQII-STVAFEQHKQYISDRVREMFSLVTTSSMSSWDTLASLLNQLQKHY 350
LF+FSLIILFPQII STVAFEQ KQ +SDRVR+++SLV T S++SW+ L L+N QKHY
Sbjct: 10 LFVFSLIILFPQIIISTVAFEQEKQTLSDRVRDIYSLVKTHSLTSWEKLKVLVNDAQKHY 69
Query: 349 FPPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXXXXXXVHKTRE 170
FPPN+DFRP+ E ++ +RM++ VHKT+E
Sbjct: 70 FPPNIDFRPKVE-EEVDEEKPNQRMKEAAKKTFGSSKSTVEKSAKSAAGAVGDAVHKTKE 128
Query: 169 KV--KDCIGDAC-HTEL 128
KV K C GDAC H EL
Sbjct: 129 KVKKKQCTGDACTHDEL 145
>KZN02701.1 hypothetical protein DCAR_011456 [Daucus carota subsp. sativus]
Length = 75
Score = 93.2 bits (230), Expect = 6e-22
Identities = 45/66 (68%), Positives = 56/66 (84%), Gaps = 1/66 (1%)
Frame = -2
Query: 526 LFIFSLIILFPQII-STVAFEQHKQYISDRVREMFSLVTTSSMSSWDTLASLLNQLQKHY 350
LF+FSLIILFPQII STVAFEQ KQ +SDRVR+++SLV T S++SW+ L L+N QKHY
Sbjct: 10 LFVFSLIILFPQIIISTVAFEQEKQTLSDRVRDIYSLVKTHSLTSWEKLKVLVNDAQKHY 69
Query: 349 FPPNLD 332
FPPN++
Sbjct: 70 FPPNIE 75
>XP_017221486.1 PREDICTED: uncharacterized protein LOC108198230 [Daucus carota
subsp. sativus]
Length = 146
Score = 94.7 bits (234), Expect = 1e-21
Identities = 59/138 (42%), Positives = 78/138 (56%), Gaps = 5/138 (3%)
Frame = -2
Query: 526 LFIFS-LIILFPQ-IISTVAFEQHKQYISDRVREMFSLVTTSSMSSWDTLASLLNQLQKH 353
L IFS ++LFP IIST A +Q KQ +SD+ R ++S+VT+SSMS W L +L+N +Q
Sbjct: 10 LLIFSAFLLLFPHVIISTAALDQEKQSVSDKFRGLYSVVTSSSMSCWQKLKTLVNDVQLR 69
Query: 352 YFPPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXXXXXXVHKTR 173
YFPP+LDFRP+ E + G+KE M++ VHKT+
Sbjct: 70 YFPPSLDFRPKVE-EEIEEQGSKEWMKEAVKKSLGSSKSTVEETAKSAASAVGEVVHKTK 128
Query: 172 EKV--KDCIGDAC-HTEL 128
EKV K C GD C H EL
Sbjct: 129 EKVAKKQCTGDGCPHEEL 146
>KZN08719.1 hypothetical protein DCAR_001375 [Daucus carota subsp. sativus]
Length = 144
Score = 85.1 bits (209), Expect = 6e-18
Identities = 55/138 (39%), Positives = 76/138 (55%), Gaps = 5/138 (3%)
Frame = -2
Query: 526 LFIFS-LIILFPQ-IISTVAFEQHKQYISDRVREMFSLVTTSSMSSWDTLASLLNQLQKH 353
L IFS ++LFP IIST A +Q KQ +SD+ R ++S+VT+SSMS W L +L+N +Q
Sbjct: 10 LLIFSAFLLLFPHVIISTAALDQEKQSVSDKFRGLYSVVTSSSMSCWQKLKTLVNDVQLR 69
Query: 352 YFPPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXXXXXXVHKTR 173
YFPP+L+ + +E + G+KE M++ VHKT+
Sbjct: 70 YFPPSLEPKVEEE---IEEQGSKEWMKEAVKKSLGSSKSTVEETAKSAASAVGEVVHKTK 126
Query: 172 EKV--KDCIGDAC-HTEL 128
EKV K C GD C H EL
Sbjct: 127 EKVAKKQCTGDGCPHEEL 144
>XP_019249558.1 PREDICTED: uncharacterized protein LOC109228806 [Nicotiana
attenuata] OIT00271.1 hypothetical protein A4A49_11108
[Nicotiana attenuata]
Length = 163
Score = 67.8 bits (164), Expect = 4e-11
Identities = 48/133 (36%), Positives = 63/133 (47%), Gaps = 10/133 (7%)
Frame = -2
Query: 520 IFSLIILFPQIISTVAFEQHKQY-ISDR---------VREMFSLVTTSSMSSWDTLASLL 371
+F LII II T + H Q I D+ + E +S++ TSS SWD + S +
Sbjct: 12 LFCLIIFSFGIIHTASDAAHYQNNIKDKQNDQSPTQMLEEAYSMLVTSSWRSWDMVKSYI 71
Query: 370 NQLQKHYFPPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXXXXX 191
NQLQ YFPPN+DFR RD+G + GA ERM++
Sbjct: 72 NQLQLKYFPPNVDFRSRDDG--KVNGGAGERMKEATQKSFEKSKETVEETAKSAAEMMEQ 129
Query: 190 XVHKTREKVKDCI 152
V KT EKVKD +
Sbjct: 130 KVQKTAEKVKDTV 142
>XP_009779351.1 PREDICTED: uncharacterized protein LOC104228571 [Nicotiana
sylvestris] XP_016509834.1 PREDICTED: uncharacterized
protein LOC107827250 [Nicotiana tabacum]
Length = 163
Score = 67.8 bits (164), Expect = 4e-11
Identities = 47/131 (35%), Positives = 63/131 (48%), Gaps = 10/131 (7%)
Frame = -2
Query: 520 IFSLIILFPQIISTVAFEQHKQY-ISDR---------VREMFSLVTTSSMSSWDTLASLL 371
+F LII II T + H Q + D+ + E +S++ TSS SWD + S +
Sbjct: 12 LFCLIIFSFGIIHTASDAAHYQNNVKDKQKDQSPTQMLEEAYSMLVTSSWRSWDMVKSYI 71
Query: 370 NQLQKHYFPPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXXXXX 191
NQLQ YFPPN+DFR RD+G ++ GA ERM++
Sbjct: 72 NQLQLKYFPPNVDFRSRDDG--KANGGAGERMKEATQKSFEKSKETVEETAKLAAEMMEQ 129
Query: 190 XVHKTREKVKD 158
V KT EKVKD
Sbjct: 130 KVQKTAEKVKD 140
>XP_016551799.1 PREDICTED: uncharacterized protein LOC107851340 [Capsicum annuum]
Length = 159
Score = 65.5 bits (158), Expect = 3e-10
Identities = 42/129 (32%), Positives = 63/129 (48%), Gaps = 6/129 (4%)
Frame = -2
Query: 526 LFIFSLIILFPQII------STVAFEQHKQYISDRVREMFSLVTTSSMSSWDTLASLLNQ 365
L +F LI + +II V +Q Q + + E +S++ TSS+ SWD + S +NQ
Sbjct: 10 LVLFCLITISSEIIHATGDQKNVQDKQKDQSPTQMLEEAYSMLVTSSLRSWDMVKSYINQ 69
Query: 364 LQKHYFPPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXXXXXXV 185
Q YFPPN+DFR +++ S ++ GA ER+ D V
Sbjct: 70 FQLKYFPPNVDFRSKED--SKANGGAGERIMDATQKSFSKSKETVEGSAKSAAEAMEQKV 127
Query: 184 HKTREKVKD 158
H+T EKVK+
Sbjct: 128 HETAEKVKN 136
>XP_016465961.1 PREDICTED: uncharacterized protein LOC107788766 [Nicotiana tabacum]
Length = 164
Score = 64.7 bits (156), Expect = 6e-10
Identities = 45/136 (33%), Positives = 63/136 (46%), Gaps = 11/136 (8%)
Frame = -2
Query: 526 LFIFSLIILFPQIISTVAFEQHKQY--ISDR---------VREMFSLVTTSSMSSWDTLA 380
+ +F LII II T + H Q + D+ + E +S++ TSS SWD
Sbjct: 10 VILFCLIIFSFGIIHTASDAAHYQNNNVKDKQKDQSPTQMLEEAYSMLVTSSWRSWDMAK 69
Query: 379 SLLNQLQKHYFPPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXX 200
S +NQ Q +FPPN+DFR RD+G ++ GA ERM++
Sbjct: 70 SYINQFQLKFFPPNVDFRSRDDG--KANGGAGERMKEATQKSFEKSKEAVEETAKSAAEM 127
Query: 199 XXXXVHKTREKVKDCI 152
V KT EKVKD +
Sbjct: 128 MEQKVQKTAEKVKDTV 143
>XP_009626113.1 PREDICTED: uncharacterized protein LOC104116868 [Nicotiana
tomentosiformis]
Length = 164
Score = 64.7 bits (156), Expect = 6e-10
Identities = 45/136 (33%), Positives = 63/136 (46%), Gaps = 11/136 (8%)
Frame = -2
Query: 526 LFIFSLIILFPQIISTVAFEQHKQY--ISDR---------VREMFSLVTTSSMSSWDTLA 380
+ +F LII II T + H Q + D+ + E +S++ TSS SWD
Sbjct: 10 VILFCLIIFSFGIIHTASDAAHYQNNNVKDKQKDQSPTQMLEEAYSMLVTSSWRSWDMAK 69
Query: 379 SLLNQLQKHYFPPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXX 200
S +NQ Q +FPPN+DFR RD+G ++ GA ERM++
Sbjct: 70 SYINQFQLKFFPPNVDFRSRDDG--KANGGAGERMKEATQKSFEKSKETVEETAKSAAEM 127
Query: 199 XXXXVHKTREKVKDCI 152
V KT EKVKD +
Sbjct: 128 MEQKVQKTAEKVKDTV 143
>XP_016483665.1 PREDICTED: uncharacterized protein LOC107804307 [Nicotiana tabacum]
Length = 140
Score = 63.2 bits (152), Expect = 1e-09
Identities = 41/122 (33%), Positives = 61/122 (50%)
Frame = -2
Query: 526 LFIFSLIILFPQIISTVAFEQHKQYISDRVREMFSLVTTSSMSSWDTLASLLNQLQKHYF 347
+FIF L+ I T K + D+ +E +S + TSS+ SWD + S +NQLQ +F
Sbjct: 10 VFIFWLVTFSFATIHTYGDAAQKN-VRDKQQEAYSTLLTSSLRSWDKVKSYINQLQLKFF 68
Query: 346 PPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXXXXXXVHKTREK 167
PP+LDF ++ +++D GAKERM++ VHKT EK
Sbjct: 69 PPDLDFGSKEV--ATADGGAKERMKEAAQKSFGKSKETVEETAKSAAKVVEQTVHKTVEK 126
Query: 166 VK 161
V+
Sbjct: 127 VR 128
>XP_009598905.1 PREDICTED: uncharacterized protein LOC104094647 [Nicotiana
tomentosiformis]
Length = 140
Score = 60.1 bits (144), Expect = 2e-08
Identities = 40/122 (32%), Positives = 60/122 (49%)
Frame = -2
Query: 526 LFIFSLIILFPQIISTVAFEQHKQYISDRVREMFSLVTTSSMSSWDTLASLLNQLQKHYF 347
+FIF L+ I T K + D+ +E +S + TSS+ SWD + S +NQLQ +F
Sbjct: 10 VFIFWLVTFSFATIHTYGDAAQKN-VRDKQQEAYSTLLTSSLRSWDKVKSYINQLQLKFF 68
Query: 346 PPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXXXXXXVHKTREK 167
P +LDF ++ +++D GAKERM++ VHKT EK
Sbjct: 69 PADLDFGSKEV--ATADGGAKERMKEAAQKSFGKSKETVEETAKSAAKVVEQTVHKTVEK 126
Query: 166 VK 161
V+
Sbjct: 127 VR 128
>XP_009779353.1 PREDICTED: uncharacterized protein LOC104228573 [Nicotiana
sylvestris]
Length = 116
Score = 59.3 bits (142), Expect = 2e-08
Identities = 34/94 (36%), Positives = 48/94 (51%)
Frame = -2
Query: 439 VREMFSLVTTSSMSSWDTLASLLNQLQKHYFPPNLDFRPRDEGHSSSDDGAKERMQDXXX 260
+ E +S++ TSS SWD + S ++QLQ +FPPN+D R RD+G ++ GA ERM++
Sbjct: 2 LEEAYSMLVTSSWRSWDMVKSYISQLQLKFFPPNVDLRSRDDG--KANGGAGERMKEATQ 59
Query: 259 XXXXXXXXXXXXXXXXXXXXXXXXVHKTREKVKD 158
V KT EKVKD
Sbjct: 60 KSFEKSKETVEETAKLAAEMMEQKVQKTAEKVKD 93
>XP_007048138.2 PREDICTED: uncharacterized protein LOC18611688 [Theobroma cacao]
Length = 149
Score = 58.9 bits (141), Expect = 7e-08
Identities = 36/131 (27%), Positives = 57/131 (43%), Gaps = 4/131 (3%)
Frame = -2
Query: 526 LFIFSLIILFPQIISTVA----FEQHKQYISDRVREMFSLVTTSSMSSWDTLASLLNQLQ 359
LF+ I+L QI+S V + + K + V S++ S SSW+ + ++++ Q
Sbjct: 11 LFLLWFIVLSHQILSVVCQRAQYNEEKPSLFQVVSSTISMLKKSHKSSWEKIKTIIHDFQ 70
Query: 358 KHYFPPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXXXXXXVHK 179
+ PPNLDFR +S D E M++ VHK
Sbjct: 71 LQFTPPNLDFRGTGTATASGSDSVGENMKEAVKKSIGTSKVTVEETAKSAAEIAEGAVHK 130
Query: 178 TREKVKDCIGD 146
T+EKVK+ + D
Sbjct: 131 TKEKVKEIVSD 141
>EOX92295.1 Uncharacterized protein TCM_001261 [Theobroma cacao]
Length = 165
Score = 58.9 bits (141), Expect = 9e-08
Identities = 36/131 (27%), Positives = 57/131 (43%), Gaps = 4/131 (3%)
Frame = -2
Query: 526 LFIFSLIILFPQIISTVA----FEQHKQYISDRVREMFSLVTTSSMSSWDTLASLLNQLQ 359
LF+ I+L QI+S V + + K + V S++ S SSW+ + ++++ Q
Sbjct: 27 LFLLWFIVLSHQILSVVCQRAQYNEEKPSLFQVVSSTISMLKKSHKSSWEKIKTIIHDFQ 86
Query: 358 KHYFPPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXXXXXXVHK 179
+ PPNLDFR +S D E M++ VHK
Sbjct: 87 LQFTPPNLDFRGTGTATASGSDSVGENMKEAVKKSIGTSKVTVEETAKSAAEIAEGAVHK 146
Query: 178 TREKVKDCIGD 146
T+EKVK+ + D
Sbjct: 147 TKEKVKEIVSD 157
>XP_015071800.1 PREDICTED: uncharacterized protein LOC107015887 [Solanum pennellii]
Length = 159
Score = 58.5 bits (140), Expect = 1e-07
Identities = 43/138 (31%), Positives = 64/138 (46%), Gaps = 6/138 (4%)
Frame = -2
Query: 526 LFIFSLIILFPQIISTVAFEQH------KQYISDRVREMFSLVTTSSMSSWDTLASLLNQ 365
L IF LI + +II T +Q+ +Q + + E +S++ TS++ S D S +NQ
Sbjct: 10 LVIFCLITISSEIIHTNGNQQNVEEDKKEQSPTQILEEAYSMLLTSTLRSLDVAKSYINQ 69
Query: 364 LQKHYFPPNLDFRPRDEGHSSSDDGAKERMQDXXXXXXXXXXXXXXXXXXXXXXXXXXXV 185
LQ YFPPN+DFR D+ S++ GA R+ + V
Sbjct: 70 LQLKYFPPNVDFRSNDD--VSANGGAGGRIMEATQKSFEKSKETVKGSAKSAAEAVEQKV 127
Query: 184 HKTREKVKDCIGDACHTE 131
H T +KVKD I T+
Sbjct: 128 HDTADKVKDTISAGRKTD 145
>XP_018810759.1 PREDICTED: uncharacterized protein LOC108983545 [Juglans regia]
Length = 158
Score = 57.8 bits (138), Expect = 2e-07
Identities = 39/146 (26%), Positives = 60/146 (41%), Gaps = 14/146 (9%)
Frame = -2
Query: 526 LFIFSLIILFPQIISTVAFEQHKQYISDR----------VREMFSLVTTSSMSSWDTLAS 377
LF+F II+ QI+ T+ EQH Y V + S++ S SW + +
Sbjct: 10 LFLFWFIIVSQQILPTIGGEQHHDYAGQNQEEKPSFAKMVTDTISILKQSHQRSWSKVKT 69
Query: 376 LLNQLQKHYFPPNLDFRPRDEGHSSSDDG----AKERMQDXXXXXXXXXXXXXXXXXXXX 209
++ +L+ FPPNLDFR D+ +S G A +++D
Sbjct: 70 IIKELEFQIFPPNLDFRAADDANSKDRVGGINSASGKVKDAAQKSFRTSKNTVEETAKSA 129
Query: 208 XXXXXXXVHKTREKVKDCIGDACHTE 131
+HKT K KD + D +E
Sbjct: 130 SEVVGGTMHKTAGKAKDNLSDKDESE 155
>XP_019254030.1 PREDICTED: uncharacterized protein LOC109232745 [Nicotiana
attenuata] OIS98502.1 hypothetical protein A4A49_25897
[Nicotiana attenuata]
Length = 138
Score = 54.7 bits (130), Expect = 2e-06
Identities = 31/92 (33%), Positives = 47/92 (51%)
Frame = -2
Query: 436 REMFSLVTTSSMSSWDTLASLLNQLQKHYFPPNLDFRPRDEGHSSSDDGAKERMQDXXXX 257
+E +S + TSS+ WD + S +NQLQ +FPP+LDFR ++ + +D GA ER+++
Sbjct: 37 QETYSTLVTSSLRYWDNVKSYINQLQLKFFPPDLDFRSKEVAY--ADGGATERVKEAAQK 94
Query: 256 XXXXXXXXXXXXXXXXXXXXXXXVHKTREKVK 161
VHKT EKV+
Sbjct: 95 SFGKSKETVEETAKSAAKVVEQTVHKTVEKVR 126
>XP_004237464.1 PREDICTED: uncharacterized protein LOC101257462 [Solanum
lycopersicum]
Length = 159
Score = 54.3 bits (129), Expect = 4e-06
Identities = 34/92 (36%), Positives = 53/92 (57%), Gaps = 6/92 (6%)
Frame = -2
Query: 526 LFIFSLIILFPQIISTVAFEQH------KQYISDRVREMFSLVTTSSMSSWDTLASLLNQ 365
L IF LI + +II T +Q+ +Q + + E +S++ TS++ S D S +NQ
Sbjct: 10 LVIFCLITISCEIIHTNGNQQNVEEDKKEQSPTQILEEAYSMLLTSTLRSLDVAKSYINQ 69
Query: 364 LQKHYFPPNLDFRPRDEGHSSSDDGAKERMQD 269
LQ YFPPN+DFR D+ S++ GA R+ +
Sbjct: 70 LQLKYFPPNVDFRSNDD--VSANKGAGGRIME 99
>XP_008356960.1 PREDICTED: uncharacterized protein LOC103420692 isoform X1 [Malus
domestica]
Length = 169
Score = 53.5 bits (127), Expect = 9e-06
Identities = 42/160 (26%), Positives = 68/160 (42%), Gaps = 27/160 (16%)
Frame = -2
Query: 526 LFIFSLIIL---FPQIISTVAFEQHKQYISDR--------------VREMFSLVTTSSMS 398
LF+F II+ P++I E K +D+ + + S++ S +
Sbjct: 10 LFLFWFIIVSLQIPKMIGEAQPEVEKNEEADQQTVFGRTMQDTMSMLYDTISVLQKSHQN 69
Query: 397 SWDTLASLLNQLQKHYFPPNLDFRPRD----EGHSSSDDGAKERMQDXXXXXXXXXXXXX 230
+WD + +++N +Q + PPNLDFR RD +G D G +E++++
Sbjct: 70 AWDKVKTIINDMQLQFSPPNLDFRGRDKANAKGSEGDDQGMREKVKEAAQKSFKTSKQTV 129
Query: 229 XXXXXXXXXXXXXXVHKTREKVKDCIGD------ACHTEL 128
VHKT EKVK+ + A H EL
Sbjct: 130 EEXAISAGEAVRETVHKTEEKVKETVASPHEETPAAHAEL 169