BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Paeonia24_contig00007022
         (1395 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_007221780.1| hypothetical protein PRUPE_ppa007504mg [Prun...   489   e-136
ref|XP_007018965.1| Holocarboxylase synthase 1 isoform 1 [Theobr...   480   e-133
ref|XP_002301010.2| hypothetical protein POPTR_0002s08830g [Popu...   478   e-132
ref|XP_006472717.1| PREDICTED: biotin--protein ligase-like [Citr...   477   e-132
ref|XP_006434124.1| hypothetical protein CICLE_v10001583mg [Citr...   476   e-132
ref|XP_004500594.1| PREDICTED: biotin--protein ligase-like [Cice...   471   e-130
ref|XP_004290831.1| PREDICTED: biotin--protein ligase-like [Frag...   471   e-130
emb|CBI19072.3| unnamed protein product [Vitis vinifera]              464   e-128
ref|XP_002285834.1| PREDICTED: biotin--protein ligase [Vitis vin...   462   e-127
ref|XP_006296439.1| hypothetical protein CARUB_v10025619mg [Caps...   462   e-127
ref|XP_003601098.1| Holocarboxylase synthetase hcs2 [Medicago tr...   460   e-127
ref|XP_003523464.1| PREDICTED: biotin--protein ligase [Glycine max]   459   e-126
ref|XP_007137367.1| hypothetical protein PHAVU_009G121400g [Phas...   456   e-125
ref|XP_004150602.1| PREDICTED: biotin--protein ligase-like [Cucu...   456   e-125
ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis ly...   455   e-125
ref|XP_002307419.1| holocarboxylase synthetase 2 family protein ...   454   e-125
ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thalian...   454   e-125
gb|AAL93108.1|AF414937_1 holocarboxylase synthetase 1 [Arabidops...   454   e-125
ref|XP_002878877.1| holocarboxylase synthetase 1 [Arabidopsis ly...   451   e-124
ref|NP_850957.1| holocarboxylase synthetase 2 [Arabidopsis thali...   447   e-123

>ref|XP_007221780.1| hypothetical protein PRUPE_ppa007504mg [Prunus persica]
            gi|462418716|gb|EMJ22979.1| hypothetical protein
            PRUPE_ppa007504mg [Prunus persica]
          Length = 365

 Score =  489 bits (1260), Expect = e-136
 Identities = 246/357 (68%), Positives = 290/357 (81%), Gaps = 3/357 (0%)
 Frame = -2

Query: 1280 LSFLSLHKRIFTNKKQFDPSLSV--AATAMDCSSSCMLVLCGKSSVETELAQSLKNNNAL 1107
            L F S  K++  + K+ + SL +  A +AMD +SSC LVLCGKSS E E A++LKN+ AL
Sbjct: 9    LGFFSALKKVILSTKKTNSSLPLPFAVSAMDGNSSCALVLCGKSSAENETAKTLKNSGAL 68

Query: 1106 KLPDDGKVSIFLHSEIEKQFEDGSFNVESFMNSLSTARFGRFLIWSPRLPSTHDVVSHN- 930
            KL D+ ++SI LHSE  K  ++ +F V+SFMNSLST RFGRFL+WSPRLPSTHDV+S N 
Sbjct: 69   KLTDNTELSILLHSESAKPIKEDAFGVDSFMNSLSTNRFGRFLLWSPRLPSTHDVISQNF 128

Query: 929  PELPVGSVCVADVQSSGRGRSKNVWESPPGCLMFSFTVQLDDARIGVLLQYVVSIAITEA 750
             ELP+G+VCVADVQ  GRGRSKNVWESP GCL+FSFT+Q++D R+  L+QYVVS+A+TEA
Sbjct: 129  CELPIGAVCVADVQYKGRGRSKNVWESPKGCLLFSFTLQMEDGRVVPLIQYVVSLAVTEA 188

Query: 749  IKDVCDSHGLPLLNVKIKWPNDLFLNGLKVGGVLCTSTYRSKKLNVRAGIGLNVDNEKPT 570
            IKDVCD +GLP ++VKIKWPNDL+LNGLKVGG+LCTST++SK+ NV AGIGLNVDNEKPT
Sbjct: 189  IKDVCDKNGLPYVDVKIKWPNDLYLNGLKVGGILCTSTFKSKQFNVSAGIGLNVDNEKPT 248

Query: 569  TCLNSALRELSSGAYQFRRXXXXXXXXXXXXXXXXXXXNQGFQTLEELYYKTWLHSGQRV 390
            TCLN+ LRELS   YQFRR                   NQGFQ+LEELYYKTWLHSGQRV
Sbjct: 249  TCLNTFLRELSVTTYQFRREDILAAFFDKFEKFYELFINQGFQSLEELYYKTWLHSGQRV 308

Query: 389  IVQEKNEDQLVENVVTIQGLTASGYLLAIGDDYQMYELHPDGNSFDFFKGLVRRKLD 219
            IVQEKN+DQ+VENVVTIQGLT+SGYLLAIGDD QM ELHPDGNSFDFFKGLVR+KLD
Sbjct: 309  IVQEKNDDQVVENVVTIQGLTSSGYLLAIGDDNQMCELHPDGNSFDFFKGLVRQKLD 365


>ref|XP_007018965.1| Holocarboxylase synthase 1 isoform 1 [Theobroma cacao]
            gi|508724293|gb|EOY16190.1| Holocarboxylase synthase 1
            isoform 1 [Theobroma cacao]
          Length = 375

 Score =  480 bits (1235), Expect = e-133
 Identities = 252/380 (66%), Positives = 292/380 (76%), Gaps = 2/380 (0%)
 Frame = -2

Query: 1352 MPFTSSLRLFSIHKSLQVLSLQNPLSFLSLHKRIFTNKKQFDPSLSVAATAMDCSSSCML 1173
            M  +S  RL S H S  VLSL+N         R+         S SVAA+AM+ SS   L
Sbjct: 10   MLVSSRFRLLSPHISFSVLSLKNHTP-----SRL---------SFSVAASAMETSSPSAL 55

Query: 1172 VLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEK-QFEDGSFNVESFMNSLSTA 996
             LCGKSS ETE+A+SLK NNALKLP+ G+VS  L SEI   Q  + +FNV  F+NSLST 
Sbjct: 56   FLCGKSSSETEIAKSLKINNALKLPNGGEVSTHLQSEISNFQLGEEAFNVNLFLNSLSTN 115

Query: 995  RFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKNVWESPPGCLMFSFT 819
            RFGR LIWSPRLPSTHDVVSHN  ELP+G+ CVADVQ  GRGRSKN WESP GCLMFSFT
Sbjct: 116  RFGRNLIWSPRLPSTHDVVSHNFCELPIGAACVADVQFKGRGRSKNAWESPRGCLMFSFT 175

Query: 818  VQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDLFLNGLKVGGVLCTS 639
            ++++D +I  L+QYVVS+A+TEAIKD+CD  G P L+V+IKWPNDL+LNGLKVGG+LCTS
Sbjct: 176  LEMEDGKIVPLVQYVVSLALTEAIKDICDREGFPCLDVRIKWPNDLYLNGLKVGGILCTS 235

Query: 638  TYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXXXXXXXXXXXXXXXX 459
            TY+SKK NV +G+GLNVDNEKPTTCLN+ L+ELS+ + +FRR                  
Sbjct: 236  TYKSKKFNVSSGVGLNVDNEKPTTCLNAVLKELSTTSNKFRREDIMAAFFNKFEMLYDIF 295

Query: 458  XNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTASGYLLAIGDDYQMYE 279
             NQGFQTLEELYY+TWLHSGQRVIVQEKNED++VENVVTIQGLT+SGYLLAIGDDYQM E
Sbjct: 296  LNQGFQTLEELYYRTWLHSGQRVIVQEKNEDRIVENVVTIQGLTSSGYLLAIGDDYQMCE 355

Query: 278  LHPDGNSFDFFKGLVRRKLD 219
            LHPDGNSFDFFKGLVRRKL+
Sbjct: 356  LHPDGNSFDFFKGLVRRKLE 375


>ref|XP_002301010.2| hypothetical protein POPTR_0002s08830g [Populus trichocarpa]
            gi|550344587|gb|EEE80283.2| hypothetical protein
            POPTR_0002s08830g [Populus trichocarpa]
          Length = 390

 Score =  478 bits (1229), Expect = e-132
 Identities = 254/372 (68%), Positives = 293/372 (78%), Gaps = 9/372 (2%)
 Frame = -2

Query: 1307 LQVLSLQNPLSFLSLHKRIF--TNKKQFDPSLSVAA-TAMDCSSS--CMLVLCGKSSVET 1143
            L+ LS Q+ LS  SLH  I   + K  F  S SVAA TAMD SSS  C LVLCGKSS E 
Sbjct: 19   LRFLSPQSSLSLRSLHNFIAPTSQKSPFTLSRSVAAATAMDSSSSTPCKLVLCGKSSAEN 78

Query: 1142 ELAQSLKNNNALKLPDDGKVSIFLHSEI---EKQFEDGSFNVESFMNSLSTARFGRFLIW 972
            E+A+SL NNN LKLPD+ +VS  LHSEI   +++ ++ SF +E FMNSLST +FGR LIW
Sbjct: 79   EIAKSLMNNNTLKLPDNVQVSTLLHSEIINKQQRQDEESFCIERFMNSLSTNQFGRLLIW 138

Query: 971  SPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKNVWESPPGCLMFSFTVQLDDARI 795
            SP LPSTHDVVS+N  ELP+G+VCVADVQ  GRGRSKNVWESP GCLMFSFT+Q++D R+
Sbjct: 139  SPILPSTHDVVSNNFGELPIGTVCVADVQYKGRGRSKNVWESPAGCLMFSFTIQMEDGRV 198

Query: 794  GVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDLFLNGLKVGGVLCTSTYRSKKLN 615
              LLQYVVS+A+TEAIKDVCD +GLP ++V+IKWPNDL+LNGLKVGG+L TSTY+SKK N
Sbjct: 199  VPLLQYVVSLAVTEAIKDVCDKNGLPRIDVRIKWPNDLYLNGLKVGGILSTSTYKSKKFN 258

Query: 614  VRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXXXXXXXXXXXXXXXXXNQGFQTL 435
            V  GIGLNVDNEKPTTCLN+ LRELS+ A   RR                   N GF+TL
Sbjct: 259  VSTGIGLNVDNEKPTTCLNAVLRELSAAACTLRREDIVAAFLNKFENFYDLFINGGFRTL 318

Query: 434  EELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTASGYLLAIGDDYQMYELHPDGNSF 255
            EELYYKTWLHSGQRVI+QEKNE+Q+VENVVTIQGLT+SGYLLAIG+D QM ELHPDGNSF
Sbjct: 319  EELYYKTWLHSGQRVIIQEKNENQVVENVVTIQGLTSSGYLLAIGEDNQMCELHPDGNSF 378

Query: 254  DFFKGLVRRKLD 219
            DFFKGLVRRKL+
Sbjct: 379  DFFKGLVRRKLE 390


>ref|XP_006472717.1| PREDICTED: biotin--protein ligase-like [Citrus sinensis]
          Length = 366

 Score =  477 bits (1227), Expect = e-132
 Identities = 251/379 (66%), Positives = 293/379 (77%), Gaps = 2/379 (0%)
 Frame = -2

Query: 1352 MPFTSS-LRLFSIHKSLQVLSLQNPLSFLSLHKRIFTNKKQFDPSLSVAATAMDCSSSCM 1176
            MPFT++ LRL S         L +P S  S+   +  N K+F   LS ++ AMD +SSC+
Sbjct: 1    MPFTTTHLRLIS--------PLIHPYS-RSIRSLVSANTKRF--RLSASSAAMDSNSSCV 49

Query: 1175 LVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEKQFEDGSFNVESFMNSLSTA 996
            LVL GKS  E E+A+SLK+N+ LKLPD+ +VSI L SEI KQ    SF+VE FMNSL+T 
Sbjct: 50   LVLSGKSLAENEIAESLKSNSTLKLPDNSEVSIHLQSEIVKQ---ESFDVELFMNSLATN 106

Query: 995  RFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKNVWESPPGCLMFSFT 819
            RFGR LIWSPRLPSTHDVVSHN  ELPVG+VCVADVQ  GRGRSKN WESP GCLMFSFT
Sbjct: 107  RFGRLLIWSPRLPSTHDVVSHNFCELPVGAVCVADVQFKGRGRSKNAWESPKGCLMFSFT 166

Query: 818  VQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDLFLNGLKVGGVLCTS 639
            +Q++D R+  LLQYV S+A+TEAIKDVC   GLP L++KIKWPNDL+LNG+KVGG+LCTS
Sbjct: 167  IQMEDGRVVPLLQYVASLALTEAIKDVCSRDGLPCLDIKIKWPNDLYLNGIKVGGILCTS 226

Query: 638  TYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXXXXXXXXXXXXXXXX 459
            TYR+KK NV  GIGLNV+NE+PTTCLN+ LR+LS   YQF+R                  
Sbjct: 227  TYRTKKFNVSIGIGLNVNNEEPTTCLNAVLRKLSDSTYQFQREDVIAAFFNKFETFYDTF 286

Query: 458  XNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTASGYLLAIGDDYQMYE 279
             NQGFQ+LEELYYKTWLHSGQRVI+QEKNEDQ+VENVVTIQGLT+SGYLLA+GDD QM E
Sbjct: 287  INQGFQSLEELYYKTWLHSGQRVIIQEKNEDQVVENVVTIQGLTSSGYLLAMGDDNQMCE 346

Query: 278  LHPDGNSFDFFKGLVRRKL 222
            LHPDGNS DFFKGL+RRKL
Sbjct: 347  LHPDGNSLDFFKGLIRRKL 365


>ref|XP_006434124.1| hypothetical protein CICLE_v10001583mg [Citrus clementina]
            gi|557536246|gb|ESR47364.1| hypothetical protein
            CICLE_v10001583mg [Citrus clementina]
          Length = 366

 Score =  476 bits (1226), Expect = e-132
 Identities = 254/381 (66%), Positives = 289/381 (75%), Gaps = 4/381 (1%)
 Frame = -2

Query: 1352 MPFTSS-LRLFS--IHKSLQVLSLQNPLSFLSLHKRIFTNKKQFDPSLSVAATAMDCSSS 1182
            MPFT++ LRL S  IH S             S+   +  N K F   LS ++ AMD +SS
Sbjct: 1    MPFTTTHLRLISPLIHPSSP-----------SIRSLVSANTKPF--RLSASSAAMDSNSS 47

Query: 1181 CMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEKQFEDGSFNVESFMNSLS 1002
            CMLVL GKS  E E+A+SLK+N+ LKLPD+ +VSI L SEI KQ    SF+VE FMNSL+
Sbjct: 48   CMLVLSGKSLAENEIAESLKSNSTLKLPDNSEVSIHLQSEIVKQ---ESFDVELFMNSLA 104

Query: 1001 TARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKNVWESPPGCLMFS 825
            T RFGR LIWSPRLPSTHDVVSHN  ELPVG+VCVADVQ  GRGRSKN WESP GCLMFS
Sbjct: 105  TNRFGRLLIWSPRLPSTHDVVSHNFCELPVGAVCVADVQFKGRGRSKNAWESPKGCLMFS 164

Query: 824  FTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDLFLNGLKVGGVLC 645
            FT+Q++D R+  LLQYV S+A+TEAI  VC   GLP L++KIKWPNDL+LNG+KVGG+LC
Sbjct: 165  FTIQMEDGRVVPLLQYVASLALTEAINYVCSRDGLPCLDIKIKWPNDLYLNGIKVGGILC 224

Query: 644  TSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXXXXXXXXXXXXXX 465
            TSTYR+KK NV  GIGLNV+NE+PTTCLN+ LR+LS   YQFRR                
Sbjct: 225  TSTYRTKKFNVSIGIGLNVNNEEPTTCLNAVLRKLSDSTYQFRREDVIAAFFNKFETFYD 284

Query: 464  XXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTASGYLLAIGDDYQM 285
               NQGFQTLEELYYKTWLHSGQRVIVQEKNEDQ+VENVVTIQGLT+SGYLLAIGDD QM
Sbjct: 285  TFINQGFQTLEELYYKTWLHSGQRVIVQEKNEDQVVENVVTIQGLTSSGYLLAIGDDNQM 344

Query: 284  YELHPDGNSFDFFKGLVRRKL 222
             ELHPDGNS DFFKGL+RRKL
Sbjct: 345  CELHPDGNSLDFFKGLIRRKL 365


>ref|XP_004500594.1| PREDICTED: biotin--protein ligase-like [Cicer arietinum]
          Length = 347

 Score =  471 bits (1213), Expect = e-130
 Identities = 237/344 (68%), Positives = 281/344 (81%), Gaps = 2/344 (0%)
 Frame = -2

Query: 1244 NKKQFDPSLS-VAATAMDCSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLH 1068
            +++ F+  LS +A++A+DC S   LVLCGKS+ E E A ++K NN LKLPD G++S+ LH
Sbjct: 7    SRRVFNYHLSTMASSALDCIS---LVLCGKSNAEIETAIAIKANNTLKLPDKGELSLVLH 63

Query: 1067 SEIEKQFEDGSFNVESFMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADV 891
            SE+ K  E  SF V SFMNSLST +FGR LIWSPRL STHDVVS+N  ELP+G+VCVAD+
Sbjct: 64   SELNKSVEQNSFQVSSFMNSLSTNQFGRLLIWSPRLTSTHDVVSNNFCELPLGTVCVADI 123

Query: 890  QSSGRGRSKNVWESPPGCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLL 711
            Q+ GRGRSKNVWESP GCLMFSFT+Q++D R+  L+QYVVS+AITEAIKD+CD +GLP +
Sbjct: 124  QNKGRGRSKNVWESPLGCLMFSFTLQMEDGRVVPLVQYVVSLAITEAIKDICDKNGLPCI 183

Query: 710  NVKIKWPNDLFLNGLKVGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSG 531
            +VKIKWPNDL+LNG KVGG+LCTSTY+SKK N+ AGIGLNV NEKPTT LN+ LRELS G
Sbjct: 184  DVKIKWPNDLYLNGFKVGGILCTSTYKSKKFNISAGIGLNVHNEKPTTSLNTVLRELSVG 243

Query: 530  AYQFRRXXXXXXXXXXXXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVEN 351
            AYQF+R                   NQGFQTLEELYYKTWLHSGQRV+VQEKNED++VE+
Sbjct: 244  AYQFQREDVLAAFFNKFEIFFDLFVNQGFQTLEELYYKTWLHSGQRVVVQEKNEDKVVEH 303

Query: 350  VVTIQGLTASGYLLAIGDDYQMYELHPDGNSFDFFKGLVRRKLD 219
            VVT+QGLT+SGYLLAIGDD QM ELHPDGNSFDFFKGLVRRKL+
Sbjct: 304  VVTVQGLTSSGYLLAIGDDNQMCELHPDGNSFDFFKGLVRRKLN 347


>ref|XP_004290831.1| PREDICTED: biotin--protein ligase-like [Fragaria vesca subsp. vesca]
          Length = 329

 Score =  471 bits (1211), Expect = e-130
 Identities = 233/328 (71%), Positives = 273/328 (83%), Gaps = 2/328 (0%)
 Frame = -2

Query: 1199 MDCSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEKQFEDG-SFNVE 1023
            MDC+SSCMLVL GKS  E ++A++LKN  ALKL DD ++SI L SE EK F++G +F V+
Sbjct: 1    MDCNSSCMLVLRGKSPAENKIAEALKNTKALKLSDDTELSILLDSESEKPFKEGEAFGVD 60

Query: 1022 SFMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKNVWESP 846
            SFM SLST RFGRFL+WSPRLPST DVVS N  ELP G+VCVADVQ  GRGRSKNVWESP
Sbjct: 61   SFMKSLSTDRFGRFLLWSPRLPSTQDVVSQNFCELPNGAVCVADVQFKGRGRSKNVWESP 120

Query: 845  PGCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDLFLNGL 666
             GCL+FSFT+Q++D R+  L+QYVVS+AITEAIKDVCD +GLP ++V+IKWPNDL+LNGL
Sbjct: 121  KGCLLFSFTLQMEDGRVVPLIQYVVSLAITEAIKDVCDRNGLPYIDVRIKWPNDLYLNGL 180

Query: 665  KVGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXXXXXXX 486
            KVGG+LCTSTY+SKK NV AG+GLN++NEKPTTCLN+ LRELS+  YQFRR         
Sbjct: 181  KVGGILCTSTYKSKKFNVSAGVGLNINNEKPTTCLNAVLRELSATTYQFRREEILAAFFN 240

Query: 485  XXXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTASGYLLA 306
                      +QGFQ+LEELYYKTWLHSGQRVIVQEKN+DQ+VENVVT+QGLT++GYLLA
Sbjct: 241  KFEKFYDLFMHQGFQSLEELYYKTWLHSGQRVIVQEKNDDQVVENVVTVQGLTSAGYLLA 300

Query: 305  IGDDYQMYELHPDGNSFDFFKGLVRRKL 222
            IGDD QM ELHPDGNSFDFFKGLVR+KL
Sbjct: 301  IGDDNQMCELHPDGNSFDFFKGLVRQKL 328


>emb|CBI19072.3| unnamed protein product [Vitis vinifera]
          Length = 416

 Score =  464 bits (1195), Expect = e-128
 Identities = 249/386 (64%), Positives = 287/386 (74%), Gaps = 9/386 (2%)
 Frame = -2

Query: 1349 PFTSSLRLFSIHKSLQVLSLQNPLS-FLSLHKRIFTNKKQFDPSLS-------VAATAMD 1194
            PF      F   +SL V   + P   F S+ + + T++ +    L        + + AMD
Sbjct: 32   PFPPFANSFIHRRSLAVYLYRLPHQLFGSVFQCLLTDQAECKRKLGRKFEFGLLCSLAMD 91

Query: 1193 CSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEKQFEDGSFNVESFM 1014
              S+ +LVLCGKSS E E A+SLK  N LKLPD+G+VSI LH EIEK  E+  F ++ FM
Sbjct: 92   SQSTSLLVLCGKSSAEIEFAKSLKAKNTLKLPDNGEVSILLHPEIEKP-EEEPFQMDLFM 150

Query: 1013 NSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKNVWESPPGC 837
            +SLST  FGRFLIWSP+LPSTHDVVS N  ELP+G+VCVADVQ  GRGRSKN WESP GC
Sbjct: 151  SSLSTNTFGRFLIWSPQLPSTHDVVSQNFCELPIGTVCVADVQLKGRGRSKNKWESPRGC 210

Query: 836  LMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDLFLNGLKVG 657
            L+FSFT+Q++D R   LLQYVVS+AITEAIK +CDS GLP L+VKIKWPNDL+LNGLKVG
Sbjct: 211  LLFSFTLQMEDGRTVPLLQYVVSLAITEAIKSICDSKGLPHLDVKIKWPNDLYLNGLKVG 270

Query: 656  GVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXXXXXXXXXX 477
            G+LCTSTY+S+K NV AGIGLNVDNEKPTTCLN+ LRELSS AYQFRR            
Sbjct: 271  GILCTSTYKSRKFNVSAGIGLNVDNEKPTTCLNAVLRELSSVAYQFRREDIVAAFFNKFE 330

Query: 476  XXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTASGYLLAIGD 297
                   NQGFQTLEELY KTWLHSGQRVIVQE  E Q+VENVVTIQGL +SGYLLAIGD
Sbjct: 331  TFFSLFINQGFQTLEELYCKTWLHSGQRVIVQENIEGQMVENVVTIQGLASSGYLLAIGD 390

Query: 296  DYQMYELHPDGNSFDFFKGLVRRKLD 219
            D QM ELHPDGNSFDFFKGL+RRKL+
Sbjct: 391  DGQMCELHPDGNSFDFFKGLIRRKLE 416


>ref|XP_002285834.1| PREDICTED: biotin--protein ligase [Vitis vinifera]
          Length = 327

 Score =  462 bits (1189), Expect = e-127
 Identities = 237/328 (72%), Positives = 265/328 (80%), Gaps = 1/328 (0%)
 Frame = -2

Query: 1199 MDCSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEKQFEDGSFNVES 1020
            MD  S+ +LVLCGKSS E E A+SLK  N LKLPD+G+VSI LH EIEK  E+  F ++ 
Sbjct: 1    MDSQSTSLLVLCGKSSAEIEFAKSLKAKNTLKLPDNGEVSILLHPEIEKP-EEEPFQMDL 59

Query: 1019 FMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKNVWESPP 843
            FM+SLST  FGRFLIWSP+LPSTHDVVS N  ELP+G+VCVADVQ  GRGRSKN WESP 
Sbjct: 60   FMSSLSTNTFGRFLIWSPQLPSTHDVVSQNFCELPIGTVCVADVQLKGRGRSKNKWESPR 119

Query: 842  GCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDLFLNGLK 663
            GCL+FSFT+Q++D R   LLQYVVS+AITEAIK +CDS GLP L+VKIKWPNDL+LNGLK
Sbjct: 120  GCLLFSFTLQMEDGRTVPLLQYVVSLAITEAIKSICDSKGLPHLDVKIKWPNDLYLNGLK 179

Query: 662  VGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXXXXXXXX 483
            VGG+LCTSTY+S+K NV AGIGLNVDNEKPTTCLN+ LRELSS AYQFRR          
Sbjct: 180  VGGILCTSTYKSRKFNVSAGIGLNVDNEKPTTCLNAVLRELSSVAYQFRREDIVAAFFNK 239

Query: 482  XXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTASGYLLAI 303
                     NQGFQTLEELY KTWLHSGQRVIVQE  E Q+VENVVTIQGL +SGYLLAI
Sbjct: 240  FETFFSLFINQGFQTLEELYCKTWLHSGQRVIVQENIEGQMVENVVTIQGLASSGYLLAI 299

Query: 302  GDDYQMYELHPDGNSFDFFKGLVRRKLD 219
            GDD QM ELHPDGNSFDFFKGL+RRKL+
Sbjct: 300  GDDGQMCELHPDGNSFDFFKGLIRRKLE 327


>ref|XP_006296439.1| hypothetical protein CARUB_v10025619mg [Capsella rubella]
            gi|482565147|gb|EOA29337.1| hypothetical protein
            CARUB_v10025619mg [Capsella rubella]
          Length = 357

 Score =  462 bits (1188), Expect = e-127
 Identities = 232/349 (66%), Positives = 278/349 (79%), Gaps = 6/349 (1%)
 Frame = -2

Query: 1250 FTNKKQFDP-SLSVAATAMDCSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDG-KVSI 1077
            FT+ K   P S SVAA+AM+  +SC LVLCGKSSVETE+A+ LK+ N+LK PDD  KVSI
Sbjct: 9    FTSLKLLRPLSFSVAASAMESDASCSLVLCGKSSVETEIAKRLKDKNSLKFPDDNTKVSI 68

Query: 1076 FLHSEIE---KQFEDGSFNVESFMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGS 909
            FL SE +   K  +DG FN+  FMNS+ T +FGRFLIWSPRL STHDVVSHN  ELPVGS
Sbjct: 69   FLESEAKHLVKDDDDGFFNLSLFMNSIITHQFGRFLIWSPRLSSTHDVVSHNFSELPVGS 128

Query: 908  VCVADVQSSGRGRSKNVWESPPGCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDS 729
            VCV D+Q  GRGR+KNVWESP GCLM+SF+++++D R+  L+QYVVS+A+TEA+KDVCD 
Sbjct: 129  VCVTDIQFKGRGRTKNVWESPKGCLMYSFSLEMEDGRVVPLVQYVVSLAVTEAVKDVCDK 188

Query: 728  HGLPLLNVKIKWPNDLFLNGLKVGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSAL 549
             GLP ++VKIKWPNDL+LNGLKVGG+LCTSTYRSKK +V  G+GLNVDNE+PTTCLN+ L
Sbjct: 189  KGLPYIDVKIKWPNDLYLNGLKVGGILCTSTYRSKKFHVSVGVGLNVDNEQPTTCLNAGL 248

Query: 548  RELSSGAYQFRRXXXXXXXXXXXXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNE 369
            +E+S  +    R                   +QGF++LEELYY+TWLHS QRVIV+EK E
Sbjct: 249  KEMSPASNLLNREEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEEKVE 308

Query: 368  DQLVENVVTIQGLTASGYLLAIGDDYQMYELHPDGNSFDFFKGLVRRKL 222
            DQ+V+NVVTIQGLT+SGYLLAIGDD QMYELHPDGNSFDFFKGLVRRK+
Sbjct: 309  DQVVQNVVTIQGLTSSGYLLAIGDDNQMYELHPDGNSFDFFKGLVRRKI 357


>ref|XP_003601098.1| Holocarboxylase synthetase hcs2 [Medicago truncatula]
            gi|355490146|gb|AES71349.1| Holocarboxylase synthetase
            hcs2 [Medicago truncatula]
          Length = 361

 Score =  460 bits (1184), Expect = e-127
 Identities = 233/334 (69%), Positives = 268/334 (80%), Gaps = 2/334 (0%)
 Frame = -2

Query: 1214 VAATAMDCSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEKQ-FEDG 1038
            +A++A+DCS   +LVLCGKS  E E A ++K NN LKLPD  ++SI LHSE  K   +  
Sbjct: 31   MASSAVDCS---LLVLCGKSLAENETAIAIKTNNTLKLPDKKELSIILHSEFNKSVIQRQ 87

Query: 1037 SFNVESFMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKN 861
            SF   SFMNSLST +FGR LIWSP L STHDVVSHN  ELPVG+VCV D+Q+ GRGRSKN
Sbjct: 88   SFQFSSFMNSLSTNQFGRLLIWSPELTSTHDVVSHNFCELPVGTVCVGDIQNKGRGRSKN 147

Query: 860  VWESPPGCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDL 681
             WESP GCLMFSFT+Q++D RI  L+QYVVS+AITEAIKD+CD +GLP ++VKIKWPNDL
Sbjct: 148  AWESPLGCLMFSFTLQMEDGRIVPLVQYVVSLAITEAIKDICDKNGLPCVDVKIKWPNDL 207

Query: 680  FLNGLKVGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXX 501
            +LNG KVGG+LCTSTYRSKK N+ AGIGLNV+NEKPTT LN+ LRELS GAYQF+R    
Sbjct: 208  YLNGFKVGGILCTSTYRSKKFNISAGIGLNVNNEKPTTSLNTVLRELSVGAYQFQREDVL 267

Query: 500  XXXXXXXXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTAS 321
                           NQGFQTLEELYYKTWLHSGQRVIVQEKNE+++VE+ VTIQGLT+S
Sbjct: 268  AAFFNKFEKFFDLFLNQGFQTLEELYYKTWLHSGQRVIVQEKNEEKVVEHAVTIQGLTSS 327

Query: 320  GYLLAIGDDYQMYELHPDGNSFDFFKGLVRRKLD 219
            GYLLA+GDD QM ELHPDGNSFDFFKGLVRRKLD
Sbjct: 328  GYLLAVGDDNQMCELHPDGNSFDFFKGLVRRKLD 361


>ref|XP_003523464.1| PREDICTED: biotin--protein ligase [Glycine max]
          Length = 361

 Score =  459 bits (1182), Expect = e-126
 Identities = 230/362 (63%), Positives = 283/362 (78%), Gaps = 4/362 (1%)
 Frame = -2

Query: 1292 LQNPL---SFLSLHKRIFTNKKQFDPSLSVAATAMDCSSSCMLVLCGKSSVETELAQSLK 1122
            L+NP    S LS   +   N +   P+  +A++ + CS   +LVLCGKS  E E A+++K
Sbjct: 3    LRNPALTASLLSATTKKLYNHRYSTPAAFMASSGVACS---LLVLCGKSLAENETAKAIK 59

Query: 1121 NNNALKLPDDGKVSIFLHSEIEKQFEDGSFNVESFMNSLSTARFGRFLIWSPRLPSTHDV 942
             NN LKLP+D K+S+ LHSE++K    G F V S+M+SLST +FGR L+WSP L STHDV
Sbjct: 60   TNNTLKLPEDEKLSLVLHSEMDKSVMQGFFQVHSYMDSLSTNQFGRLLVWSPDLTSTHDV 119

Query: 941  VSHN-PELPVGSVCVADVQSSGRGRSKNVWESPPGCLMFSFTVQLDDARIGVLLQYVVSI 765
            VSHN  ELP+G+VC+ADVQ+ GRGRSKNVWESP GCLMFSFT+Q++D R+  L+QYVVS+
Sbjct: 120  VSHNFCELPIGTVCIADVQTKGRGRSKNVWESPLGCLMFSFTMQMEDGRVVPLVQYVVSL 179

Query: 764  AITEAIKDVCDSHGLPLLNVKIKWPNDLFLNGLKVGGVLCTSTYRSKKLNVRAGIGLNVD 585
            A+TEAIKD+CD +GLP ++VKIKWPNDL+LNG KVGG+LCTSTY+SKK NV AGIGLNV+
Sbjct: 180  AMTEAIKDICDKNGLPSIDVKIKWPNDLYLNGSKVGGILCTSTYKSKKFNVSAGIGLNVN 239

Query: 584  NEKPTTCLNSALRELSSGAYQFRRXXXXXXXXXXXXXXXXXXXNQGFQTLEELYYKTWLH 405
            N+KPTT LN+ L+   SGAYQF+R                   NQGFQTLEELY KTWLH
Sbjct: 240  NKKPTTSLNTILKGFYSGAYQFQREEVLAAFFNKFEIFYDLFLNQGFQTLEELYIKTWLH 299

Query: 404  SGQRVIVQEKNEDQLVENVVTIQGLTASGYLLAIGDDYQMYELHPDGNSFDFFKGLVRRK 225
            SGQRV+VQEKNED+++E+VVTIQGLT+SGYLLA+GDD+QM ELHPDGNSFDFFKGLVRRK
Sbjct: 300  SGQRVVVQEKNEDKVIEHVVTIQGLTSSGYLLAVGDDHQMCELHPDGNSFDFFKGLVRRK 359

Query: 224  LD 219
            L+
Sbjct: 360  LE 361


>ref|XP_007137367.1| hypothetical protein PHAVU_009G121400g [Phaseolus vulgaris]
            gi|561010454|gb|ESW09361.1| hypothetical protein
            PHAVU_009G121400g [Phaseolus vulgaris]
          Length = 360

 Score =  456 bits (1173), Expect = e-125
 Identities = 231/362 (63%), Positives = 283/362 (78%), Gaps = 4/362 (1%)
 Frame = -2

Query: 1292 LQNP---LSFLSLHKRIFTNKKQFDPSLSVAATAMDCSSSCMLVLCGKSSVETELAQSLK 1122
            L+NP    + LS   R   N +    S S+A++A++CS   +LVL GKS  E E A+++K
Sbjct: 3    LRNPSWTTTLLSATARRLYNHR-LSTSSSMASSALECS---LLVLYGKSLAENETAKAIK 58

Query: 1121 NNNALKLPDDGKVSIFLHSEIEKQFEDGSFNVESFMNSLSTARFGRFLIWSPRLPSTHDV 942
             NN LKLP++G+ S+ LHS+++K    GSF + SFM+SLST +FGR LIWSP+L STHD+
Sbjct: 59   TNNTLKLPENGEYSLVLHSQLDKALMQGSFQIPSFMDSLSTKQFGRLLIWSPQLTSTHDI 118

Query: 941  VSHN-PELPVGSVCVADVQSSGRGRSKNVWESPPGCLMFSFTVQLDDARIGVLLQYVVSI 765
            VS N  ELP+G+VCVADVQ+ GRGRSKNVWESP GCLMFSFT+Q++D R+  L+QYVVS+
Sbjct: 119  VSQNFCELPIGTVCVADVQTKGRGRSKNVWESPSGCLMFSFTLQMEDGRVVPLVQYVVSL 178

Query: 764  AITEAIKDVCDSHGLPLLNVKIKWPNDLFLNGLKVGGVLCTSTYRSKKLNVRAGIGLNVD 585
            AITEA+KD+C  +GLP ++ KIKWPNDL+LNG KVGG+LCTSTY+SKK NV AGIGLNV+
Sbjct: 179  AITEAVKDICYKNGLPSIDAKIKWPNDLYLNGSKVGGILCTSTYKSKKFNVSAGIGLNVN 238

Query: 584  NEKPTTCLNSALRELSSGAYQFRRXXXXXXXXXXXXXXXXXXXNQGFQTLEELYYKTWLH 405
            NEKPTT LN+ LRELS+G YQF+R                   NQGFQTLEELY KTWLH
Sbjct: 239  NEKPTTSLNTVLRELSTGGYQFQREDILAAFFNKFEEFYGLFVNQGFQTLEELYIKTWLH 298

Query: 404  SGQRVIVQEKNEDQLVENVVTIQGLTASGYLLAIGDDYQMYELHPDGNSFDFFKGLVRRK 225
            SGQRV+VQEKN D+++E+VVTIQGLT+SGYLLA+GDD QM ELHPDGNSFDFFKGLVRRK
Sbjct: 299  SGQRVVVQEKNGDKVIEHVVTIQGLTSSGYLLAVGDDNQMCELHPDGNSFDFFKGLVRRK 358

Query: 224  LD 219
            LD
Sbjct: 359  LD 360


>ref|XP_004150602.1| PREDICTED: biotin--protein ligase-like [Cucumis sativus]
          Length = 328

 Score =  456 bits (1172), Expect = e-125
 Identities = 220/327 (67%), Positives = 262/327 (80%), Gaps = 1/327 (0%)
 Frame = -2

Query: 1199 MDCSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEKQFEDGSFNVES 1020
            MD S +C LVL GK++VE E A+ LK N+ LKLPDD ++S+ LHSE +K  E+  F ++ 
Sbjct: 1    MDASPTCSLVLSGKTAVENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 60

Query: 1019 FMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKNVWESPP 843
            ++N+LST  FGRFLIWSPR+PST DV+SHN   LP+G+VCVADVQ  GRGRSKN+WESPP
Sbjct: 61   YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 120

Query: 842  GCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDLFLNGLK 663
            GCLMFSFT+Q++D RI  LLQYV+S+AITEAIKD+CD  GLP +++KIKWPNDL++N LK
Sbjct: 121  GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 180

Query: 662  VGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXXXXXXXX 483
            VGGVLCTSTYR KK NV AGIGLNVDN+KP+TCLN AL  LSS  Y+FR+          
Sbjct: 181  VGGVLCTSTYRLKKFNVTAGIGLNVDNDKPSTCLNEALTNLSSTPYKFRKEDILAFFFNK 240

Query: 482  XXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTASGYLLAI 303
                     NQGF+ LEELYY+TWLHSGQRV+VQEK EDQ+VENVVTIQGLT+SGYLLAI
Sbjct: 241  FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 300

Query: 302  GDDYQMYELHPDGNSFDFFKGLVRRKL 222
            GDDYQM ELHPDGNS DFFKGL++ KL
Sbjct: 301  GDDYQMCELHPDGNSLDFFKGLIKSKL 327


>ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis lyrata subsp. lyrata]
            gi|297339768|gb|EFH70185.1| holocarboxylase synthetase 2
            [Arabidopsis lyrata subsp. lyrata]
          Length = 328

 Score =  455 bits (1170), Expect = e-125
 Identities = 225/328 (68%), Positives = 262/328 (79%), Gaps = 2/328 (0%)
 Frame = -2

Query: 1199 MDCSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEKQF-EDGSFNVE 1023
            MD  +SC LVLCGKSSVET+ A  LKNNN LKLPD+ KVSIFL SEI+    +D SFN+ 
Sbjct: 1    MDFDASCSLVLCGKSSVETDAATRLKNNNVLKLPDNTKVSIFLQSEIKNLVKDDDSFNLS 60

Query: 1022 SFMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKNVWESP 846
             FMNS+ST RFGRFLIWSP L STHDVVSHN  E+PVGSVCV D+Q  GRGR+KNVWESP
Sbjct: 61   LFMNSISTHRFGRFLIWSPFLSSTHDVVSHNFSEIPVGSVCVTDIQFKGRGRTKNVWESP 120

Query: 845  PGCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDLFLNGL 666
             GCLM+SFTV+++D R+  L+QYVVS+A+TEA+KDVCD  GL  ++VKIKWPNDL+LNGL
Sbjct: 121  KGCLMYSFTVEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYIDVKIKWPNDLYLNGL 180

Query: 665  KVGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXXXXXXX 486
            KVGG+LCTSTY S+K +V  G+GLNVDNEKPTTCLN+ L+E    +   +R         
Sbjct: 181  KVGGILCTSTYGSRKFHVSVGVGLNVDNEKPTTCLNAVLKENCPASNLLKREEILGAFFH 240

Query: 485  XXXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTASGYLLA 306
                       QGF++LEELYY+TWLHSGQRVIV+EKNEDQ+V+NVVTIQGLT+SGYLLA
Sbjct: 241  KFETFFDLFMEQGFKSLEELYYRTWLHSGQRVIVEEKNEDQVVQNVVTIQGLTSSGYLLA 300

Query: 305  IGDDYQMYELHPDGNSFDFFKGLVRRKL 222
            IGDD  MYELHPDGNSFDFFKGLVRRKL
Sbjct: 301  IGDDNVMYELHPDGNSFDFFKGLVRRKL 328


>ref|XP_002307419.1| holocarboxylase synthetase 2 family protein [Populus trichocarpa]
            gi|222856868|gb|EEE94415.1| holocarboxylase synthetase 2
            family protein [Populus trichocarpa]
          Length = 331

 Score =  454 bits (1169), Expect = e-125
 Identities = 228/331 (68%), Positives = 267/331 (80%), Gaps = 4/331 (1%)
 Frame = -2

Query: 1199 MDCSSS-CMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEI--EKQFEDGSFN 1029
            MD SS+ C L+LCGKSS E E+A+SL NNN LKLPD+ ++S   HSEI  E Q  + SF+
Sbjct: 1    MDSSSTPCKLLLCGKSSAENEIAKSLMNNNTLKLPDNVEISTLSHSEILDEPQQNEESFS 60

Query: 1028 VESFMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKNVWE 852
            +  FMNSLST  FGR LIWSP LPSTHD+VS+N  ELP+G+VC+ADVQ  GRGRSKNVWE
Sbjct: 61   LSRFMNSLSTNLFGRLLIWSPLLPSTHDLVSNNFGELPIGTVCIADVQYKGRGRSKNVWE 120

Query: 851  SPPGCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDLFLN 672
            SP GCLMFSFT+Q++D ++  LLQYVVS+A+TEAIKD+CD +GLP ++V+IKWPNDL+LN
Sbjct: 121  SPAGCLMFSFTIQMEDGQVVPLLQYVVSLAVTEAIKDLCDKNGLPHIDVRIKWPNDLYLN 180

Query: 671  GLKVGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXXXXX 492
            G+KVGG+LCTSTYRSKK N+ AGIGLNVDNEKPTTCLN+ LREL+  A   RR       
Sbjct: 181  GVKVGGILCTSTYRSKKFNISAGIGLNVDNEKPTTCLNAVLRELAGAACALRREDIVAAF 240

Query: 491  XXXXXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTASGYL 312
                        N GFQTLEELYYKTWLHSGQRVI+QEKNE+Q+VENVVTIQGLT SGYL
Sbjct: 241  LNKFEKLYDLFINGGFQTLEELYYKTWLHSGQRVIIQEKNENQVVENVVTIQGLTPSGYL 300

Query: 311  LAIGDDYQMYELHPDGNSFDFFKGLVRRKLD 219
            LAIG+D QM ELHPDGNSFDFFKGLVRRK++
Sbjct: 301  LAIGEDNQMCELHPDGNSFDFFKGLVRRKIE 331


>ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thaliana]
            gi|30682866|ref|NP_850067.1| holocarboxylase synthase 1
            [Arabidopsis thaliana] gi|75206635|sp|Q9SL92.2|HCS1_ARATH
            RecName: Full=Biotin--protein ligase 1, chloroplastic;
            AltName: Full=Holocarboxylase synthetase 1; Includes:
            RecName: Full=Biotin--[methylcrotonoyl-CoA-carboxylase]
            ligase; Includes: RecName:
            Full=Biotin--[acetyl-CoA-carboxylase] ligase; Flags:
            Precursor gi|1688072|gb|AAC49706.1| biotin
            holocarboxylase synthetase [Arabidopsis thaliana]
            gi|20197509|gb|AAD31371.2| biotin holocarboxylase
            synthetase [Arabidopsis thaliana]
            gi|89000993|gb|ABD59086.1| At2g25710 [Arabidopsis
            thaliana] gi|330252646|gb|AEC07740.1| holocarboxylase
            synthase 1 [Arabidopsis thaliana]
            gi|330252647|gb|AEC07741.1| holocarboxylase synthase 1
            [Arabidopsis thaliana]
          Length = 367

 Score =  454 bits (1167), Expect = e-125
 Identities = 221/337 (65%), Positives = 271/337 (80%), Gaps = 3/337 (0%)
 Frame = -2

Query: 1223 SLSVAATAMDCSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEK--Q 1050
            S S +A+AM+  +SC LVLCGKSSVETE+A+ LKN N+LKLPD+ KVS+ L SE +   +
Sbjct: 31   SFSFSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILESEAKNLVK 90

Query: 1049 FEDGSFNVESFMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRG 873
             +D SFN+  FMNS+ T RFGRFLIWSPRL STHDVVSHN  ELPVGSVCV D+Q  GRG
Sbjct: 91   DDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRG 150

Query: 872  RSKNVWESPPGCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKW 693
            R+KNVWESP GCLM+SFT++++D R+  L+QYVVS+A+TEA+KDVCD  GLP ++VKIKW
Sbjct: 151  RTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKW 210

Query: 692  PNDLFLNGLKVGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRR 513
            PNDL++NGLKVGG+LCTSTYRSKK NV  G+GLNVDN +PTTCLN+ L+ ++  +   +R
Sbjct: 211  PNDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKR 270

Query: 512  XXXXXXXXXXXXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQG 333
                               +QGF++LEELYY+TWLHS QRVIV++K EDQ+V+NVVTIQG
Sbjct: 271  EEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNVVTIQG 330

Query: 332  LTASGYLLAIGDDYQMYELHPDGNSFDFFKGLVRRKL 222
            LT+SGYLLA+GDD QMYELHPDGNSFDFFKGLVRRK+
Sbjct: 331  LTSSGYLLAVGDDNQMYELHPDGNSFDFFKGLVRRKI 367


>gb|AAL93108.1|AF414937_1 holocarboxylase synthetase 1 [Arabidopsis thaliana]
          Length = 367

 Score =  454 bits (1167), Expect = e-125
 Identities = 221/337 (65%), Positives = 271/337 (80%), Gaps = 3/337 (0%)
 Frame = -2

Query: 1223 SLSVAATAMDCSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEK--Q 1050
            S S +A+AM+  +SC LVLCGKSSVETE+A+ LKN N+LKLPD+ KVS+ L SE +   +
Sbjct: 31   SFSFSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILESEAKNLVK 90

Query: 1049 FEDGSFNVESFMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRG 873
             +D SFN+  FMNS+ T RFGRFLIWSPRL STHDVVSHN  ELPVGSVCV D+Q  GRG
Sbjct: 91   DDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRG 150

Query: 872  RSKNVWESPPGCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKW 693
            R+KNVWESP GCLM+SFT++++D R+  L+QYVVS+A+TEA+KDVCD  GLP ++VKIKW
Sbjct: 151  RTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKW 210

Query: 692  PNDLFLNGLKVGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRR 513
            PNDL++NGLKVGG+LCTSTYRSKK NV  G+GLNVDN +PTTCLN+ L+ ++  +   +R
Sbjct: 211  PNDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKR 270

Query: 512  XXXXXXXXXXXXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQG 333
                               +QGF++LEELYY+TWLHS QRVIV++K EDQ+V+NVVTIQG
Sbjct: 271  EEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNVVTIQG 330

Query: 332  LTASGYLLAIGDDYQMYELHPDGNSFDFFKGLVRRKL 222
            LT+SGYLLA+GDD QMYELHPDGNSFDFFKGLVRRK+
Sbjct: 331  LTSSGYLLAVGDDNQMYELHPDGNSFDFFKGLVRRKI 367


>ref|XP_002878877.1| holocarboxylase synthetase 1 [Arabidopsis lyrata subsp. lyrata]
            gi|297324716|gb|EFH55136.1| holocarboxylase synthetase 1
            [Arabidopsis lyrata subsp. lyrata]
          Length = 365

 Score =  451 bits (1160), Expect = e-124
 Identities = 223/336 (66%), Positives = 269/336 (80%), Gaps = 2/336 (0%)
 Frame = -2

Query: 1223 SLSVAATAMDCSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEKQF- 1047
            S SV+A+AM+  +SC LVL GKSSVETE+A+ LKN N+L LPD+ KVS+FL SE +    
Sbjct: 30   SFSVSASAMESDASCNLVLYGKSSVETEIAKCLKNKNSLTLPDNTKVSLFLESEAKNLVK 89

Query: 1046 EDGSFNVESFMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGR 870
            +DGSFN+  FMNS+ T RFGRFLIWSPRL STHDVVS N  ELPVGSVCV D+Q  GRGR
Sbjct: 90   DDGSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSQNFSELPVGSVCVTDIQFKGRGR 149

Query: 869  SKNVWESPPGCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWP 690
            +KNVWESP GCLM+SFT++++D R+  L+QYVVS+A+TEA+KDVCD  GLP ++VKIKWP
Sbjct: 150  TKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWP 209

Query: 689  NDLFLNGLKVGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRX 510
            NDL+LNGLKVGG+LCTSTYRSKK +V  G+GLNVDNE+PTTCLN+ L+ +S  +   +R 
Sbjct: 210  NDLYLNGLKVGGILCTSTYRSKKFHVSVGVGLNVDNEQPTTCLNAVLKGMSPESTLLKRE 269

Query: 509  XXXXXXXXXXXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGL 330
                              +QGF++LEELYY+TWLHS QRV V+EK EDQ+V+NVVTIQGL
Sbjct: 270  EILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVTVEEKVEDQVVQNVVTIQGL 329

Query: 329  TASGYLLAIGDDYQMYELHPDGNSFDFFKGLVRRKL 222
             +SGYLLAIGDD QMYELHPDGNSFDFFKGLVRRK+
Sbjct: 330  ASSGYLLAIGDDNQMYELHPDGNSFDFFKGLVRRKI 365


>ref|NP_850957.1| holocarboxylase synthetase 2 [Arabidopsis thaliana]
            gi|614084158|sp|F4I4W2.1|HCS2_ARATH RecName:
            Full=Biotin--protein ligase 2; AltName:
            Full=Holocarboxylase synthetase 2
            gi|332193776|gb|AEE31897.1| holocarboxylase synthetase 2
            [Arabidopsis thaliana]
          Length = 329

 Score =  447 bits (1150), Expect = e-123
 Identities = 220/329 (66%), Positives = 262/329 (79%), Gaps = 3/329 (0%)
 Frame = -2

Query: 1199 MDCSSSCMLVLCGKSSVETELAQSLKNNNALKLPDDGKVSIFLHSEIEK--QFEDGSFNV 1026
            MD  +SC LVL GKSSVET+ A  LKNNN LKLPD+ KVSIFL SEI+   + +D SFN+
Sbjct: 1    MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 1025 ESFMNSLSTARFGRFLIWSPRLPSTHDVVSHN-PELPVGSVCVADVQSSGRGRSKNVWES 849
              FMNS+ST RFGRFLIWSP L STHDVVSHN  E+PVGSVCV+D+Q  GRGR+KNVWES
Sbjct: 61   SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 848  PPGCLMFSFTVQLDDARIGVLLQYVVSIAITEAIKDVCDSHGLPLLNVKIKWPNDLFLNG 669
            P GCLM+SFT++++D R+  L+QYVVS+A+TEA+KDVCD  GL   +VKIKWPNDL+LNG
Sbjct: 121  PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 668  LKVGGVLCTSTYRSKKLNVRAGIGLNVDNEKPTTCLNSALRELSSGAYQFRRXXXXXXXX 489
            LK+GG+LCTSTYRS+K  V  G+GLNVDNE+PTTCLN+ L+++   +   +R        
Sbjct: 181  LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 488  XXXXXXXXXXXNQGFQTLEELYYKTWLHSGQRVIVQEKNEDQLVENVVTIQGLTASGYLL 309
                        QGF++LEELYY+TWLHSGQRVI +EKNEDQ+V+NVVTIQGLT+SGYLL
Sbjct: 241  KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLL 300

Query: 308  AIGDDYQMYELHPDGNSFDFFKGLVRRKL 222
            AIGDD  MYELHPDGNSFDFFKGLVRRKL
Sbjct: 301  AIGDDNVMYELHPDGNSFDFFKGLVRRKL 329