BLASTX nr result
ID: Paeonia23_contig00011242
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Paeonia23_contig00011242
(1063 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti... 272 2e-70
ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prun... 221 3e-55
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2... 206 1e-50
ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|50... 204 5e-50
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2... 190 1e-45
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ... 190 1e-45
ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like ... 185 3e-44
emb|CBI32348.3| unnamed protein product [Vitis vinifera] 182 2e-43
ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like ... 181 6e-43
gb|EXB39337.1| Isoamylase 2 [Morus notabilis] 179 1e-42
ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Popu... 174 4e-41
ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Sola... 165 3e-38
ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|... 165 3e-38
ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citr... 164 6e-38
ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2... 163 1e-37
ref|XP_006303226.1| hypothetical protein CARUB_v10012076mg [Caps... 153 1e-34
ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like ... 152 2e-34
ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like ... 150 9e-34
ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] gi|42571321... 150 1e-33
gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana] 149 1e-33
>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
Length = 882
Score = 272 bits (696), Expect = 2e-70
Identities = 143/237 (60%), Positives = 170/237 (71%), Gaps = 3/237 (1%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKT---EVAQKFT 532
M TL+PS+ IRP CVNCGA +S KL+ YI GNK GLEK+ + RK VAQ T
Sbjct: 1 MTTLIPSLVIRPCCVNCGARDSSKLVAATHYICGNKVAHGLEKMDLERKEILGVVAQNAT 60
Query: 533 KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 712
+N RN+N+K+ ATS I++ +TE R +T TE EL+ L+YLF TEIGG VKV VGKKN
Sbjct: 61 RNCFRNINWKVSATSHIAIEETENRYST-TESEELESRLNYLFLTEIGGQVKVIVGKKNK 119
Query: 713 KYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSS 892
KY V IEVSSL NSD++LILSWG+FRS+SSC MPVDFQN + T E PF ++SS
Sbjct: 120 KYIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFMERSS 179
Query: 893 GIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
G F L FEA P YLSFLLKS+LD D +SS+IRSHRKTNFC+PVGFG G PAPL
Sbjct: 180 GSFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPL 236
>ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prunus persica]
gi|462397150|gb|EMJ02949.1| hypothetical protein
PRUPE_ppa001199mg [Prunus persica]
Length = 883
Score = 221 bits (564), Expect = 3e-55
Identities = 121/237 (51%), Positives = 151/237 (63%), Gaps = 3/237 (1%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKT---EVAQKFT 532
MATL S+AI+ C NCG TES KL + +KA+ G K+ RK EV Q
Sbjct: 1 MATLPLSIAIQACCFNCGITESSKLTATNGFRHRDKAMHGFVKLDAERKLVFREVVQNIN 60
Query: 533 KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 712
+N R+ N K+ A SQ+SV EQR +T TE E+ + +YLFRTEIG V VFV K+N
Sbjct: 61 ENLPRDHNLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFVRKRNA 120
Query: 713 KYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSS 892
KY V IEV S ++D L+L WG++R+DSSC +P+DF++S T T+ETP Q SS
Sbjct: 121 KYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLETPLIQTSS 180
Query: 893 GIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
G FT +L FEAKQ P Y SF+L S D + + EIRSHRKTNFCVPVGFG G P PL
Sbjct: 181 GRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPL 237
>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
isoamylase, putative [Ricinus communis]
Length = 872
Score = 206 bits (525), Expect = 1e-50
Identities = 115/234 (49%), Positives = 151/234 (64%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVAQKFTKNP 541
MAT++PS AIRP CGATES KL + GNK G + + + ++P
Sbjct: 1 MATIIPSFAIRPCYCVCGATESSKLA-----VTGNKDF-GRLVLDQRLFNQRLHFWKQSP 54
Query: 542 LRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNIKYA 721
+L+ ++ + +++ V QTE R +TS + EL + +YLFRT+ GGHVKV V KKN KYA
Sbjct: 55 HWSLDSRVCSAARVPVQQTELRFSTSAPLDELNKVSTYLFRTQFGGHVKVLVRKKNAKYA 114
Query: 722 VYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSSGIF 901
VYIEVSSL +D L+L WGI+RSDSSC MP+D QN A AR ++T Q S G F
Sbjct: 115 VYIEVSSLELGTTDYRLMLIWGIYRSDSSCFMPLDSQNFAPNAR--KMDTALVQNSFGTF 172
Query: 902 TTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
+L FE KQTP YLSFLLKS L+ D++ EI++H+ NFCVP+GF SG P+PL
Sbjct: 173 ALELEFEPKQTPFYLSFLLKSKLNTDASGLEIKNHKNANFCVPIGFNSGDPSPL 226
>ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|508702938|gb|EOX94834.1|
Debranching enzyme 1 [Theobroma cacao]
Length = 867
Score = 204 bits (519), Expect = 5e-50
Identities = 119/237 (50%), Positives = 153/237 (64%), Gaps = 3/237 (1%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKT---EVAQKFT 532
MATL PS+AI PHC+N GATE KL F +Y+ +K L++I +GRK EVAQ
Sbjct: 1 MATLPPSLAIGPHCLNSGATELSKLSFVTRYLCKSKMGQSLQRIDVGRKLFSGEVAQNVM 60
Query: 533 KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 712
++ NL+ + A S++SV QTEQ + TST++ EL++ +YLFRTEIGG VKVFV K+++
Sbjct: 61 QSRYWNLDLRCFAASRVSVEQTEQ-IFTSTQVDELKKLSTYLFRTEIGGQVKVFVRKRSV 119
Query: 713 KYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSS 892
+ V IEVSSL +S+L+LS GI RSD D +N IETPF KSS
Sbjct: 120 NHVVDIEVSSLQLSGDESQLVLSGGIHRSDH------DIKNRI-------IETPFIAKSS 166
Query: 893 GIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
+L FEAK+ P Y SFLLK+ + + SEIR+HRKTNFCVPVGF G PAPL
Sbjct: 167 SELALELEFEAKEAPFYFSFLLKAPSGANLSGSEIRTHRKTNFCVPVGFNQGYPAPL 223
>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
[Cucumis sativus]
Length = 885
Score = 190 bits (482), Expect = 1e-45
Identities = 112/238 (47%), Positives = 145/238 (60%), Gaps = 4/238 (1%)
Frame = +2
Query: 362 MATLLPSVAIRPHCV-NCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVAQK---F 529
MAT L S+ I+P CV NCG TESPKL G K K+ R +
Sbjct: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
Query: 530 TKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKN 709
K+ RNL+ K A S ISV ++ QRL + +E + +YLFRTE G V VFVGKK
Sbjct: 61 VKSSHRNLS-KTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKG 119
Query: 710 IKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKS 889
+ V IEV S+ ++ D L+LSWG++RSDS+ + P +F++S TG ETPF + S
Sbjct: 120 STFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTS 178
Query: 890 SGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
G F+ +L F+AK TP YLSF+LK + VDS SSEIRSH+KT+F VPVGFG G P+PL
Sbjct: 179 EGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPL 236
>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
Length = 885
Score = 190 bits (482), Expect = 1e-45
Identities = 112/238 (47%), Positives = 145/238 (60%), Gaps = 4/238 (1%)
Frame = +2
Query: 362 MATLLPSVAIRPHCV-NCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVAQK---F 529
MAT L S+ I+P CV NCG TESPKL G K K+ R +
Sbjct: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
Query: 530 TKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKN 709
K+ RNL+ K A S ISV ++ QRL + +E + +YLFRTE G V VFVGKK
Sbjct: 61 VKSSHRNLS-KTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKG 119
Query: 710 IKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKS 889
+ V IEV S+ ++ D L+LSWG++RSDS+ + P +F++S TG ETPF + S
Sbjct: 120 STFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTS 178
Query: 890 SGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
G F+ +L F+AK TP YLSF+LK + VDS SSEIRSH+KT+F VPVGFG G P+PL
Sbjct: 179 EGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPL 236
>ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like isoform X1 [Citrus
sinensis] gi|568852193|ref|XP_006479764.1| PREDICTED:
isoamylase 2, chloroplastic-like isoform X2 [Citrus
sinensis] gi|568852195|ref|XP_006479765.1| PREDICTED:
isoamylase 2, chloroplastic-like isoform X3 [Citrus
sinensis]
Length = 889
Score = 185 bits (469), Expect = 3e-44
Identities = 109/242 (45%), Positives = 145/242 (59%), Gaps = 8/242 (3%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATES--PKLIFPAQ-----YIIGNKAICGLEKIHMGRKTEVA 520
MATL PS+A RP C NCGA ES P + A Y+ +K ++ + KT A
Sbjct: 1 MATLAPSLAFRPCCFNCGAVESSPPSKLCAASASASHYVFKSKCTRRPARLEV-EKTLSA 59
Query: 521 QKFTKNPLRNLNFKLLATS-QISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFV 697
Q +F+ A+S ++SV +T+ T +TE+ ELQ+ +YLFRT++GG V V V
Sbjct: 60 QVCQNAVTTRPDFRACASSSRVSVDETQPVFTPTTEVDELQKVSTYLFRTDLGGLVSVSV 119
Query: 698 GKKNIKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPF 877
G+KN+ YAV I+VSSL +L+L WG++ SDSS M + Q A+TG+IETPF
Sbjct: 120 GEKNVNYAVTIDVSSLQTTRGGHKLVLIWGVYTSDSSSYMHLGSQCFTPDAKTGSIETPF 179
Query: 878 TQKSSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPA 1057
T S F +L FEAKQTP YLSFLLKS L S EIR+H FCVPVG +G+P+
Sbjct: 180 TPTSFDTFALELGFEAKQTPFYLSFLLKSPLRDGSGDFEIRNHLSAKFCVPVGLNAGVPS 239
Query: 1058 PL 1063
P+
Sbjct: 240 PM 241
>emb|CBI32348.3| unnamed protein product [Vitis vinifera]
Length = 447
Score = 182 bits (462), Expect = 2e-43
Identities = 97/161 (60%), Positives = 117/161 (72%), Gaps = 3/161 (1%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKT---EVAQKFT 532
M TL+PS+ IRP CVNCGA +S KL+ YI GNK GLEK+ + RK VAQ T
Sbjct: 1 MTTLIPSLVIRPCCVNCGARDSSKLVAATHYICGNKVAHGLEKMDLERKEILGVVAQNAT 60
Query: 533 KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 712
+N RN+N+K+ ATS I++ +TE R +T TE EL+ L+YLF TEIGG VKV VGKKN
Sbjct: 61 RNCFRNINWKVSATSHIAIEETENRYST-TESEELESRLNYLFLTEIGGQVKVIVGKKNK 119
Query: 713 KYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQN 835
KY V IEVSSL NSD++LILSWG+FRS+SSC MPVDFQN
Sbjct: 120 KYIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQN 160
>ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like [Fragaria vesca subsp.
vesca]
Length = 868
Score = 181 bits (458), Expect = 6e-43
Identities = 105/232 (45%), Positives = 135/232 (58%), Gaps = 4/232 (1%)
Frame = +2
Query: 380 SVAIRPHCVNCGAT-ESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVA---QKFTKNPLR 547
S+ + C CG T ES KL Y K GL K+ RK Q F ++ LR
Sbjct: 4 SITPQACCWACGTTSESSKLTANTHYRDRKKTTLGLVKLDAERKLVFGGFGQSFAQSSLR 63
Query: 548 NLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNIKYAVY 727
+ ++ A S +S+ EQ T TE E + +YLFRTE G + VFV + Y+VY
Sbjct: 64 GCHSRVQAASGVSIEPMEQNFPTGTE--ETNKVSTYLFRTENGDLINVFVRDNTVNYSVY 121
Query: 728 IEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSSGIFTT 907
+E+SSL ++ L++SWG++R+DSS L P+DF IETPFT+ SSG FT
Sbjct: 122 VELSSLQLSSAGDRLVISWGMYRADSSSLKPLDF-----------IETPFTKTSSGSFTL 170
Query: 908 DLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
+L FEAKQTP YLSF +KS D + + EIRSHRKTNFCVPVGFG G P PL
Sbjct: 171 ELEFEAKQTPFYLSFTVKSLADANLSGLEIRSHRKTNFCVPVGFGRGCPTPL 222
>gb|EXB39337.1| Isoamylase 2 [Morus notabilis]
Length = 825
Score = 179 bits (455), Expect = 1e-42
Identities = 94/179 (52%), Positives = 123/179 (68%)
Frame = +2
Query: 527 FTKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKK 706
F K P RNL+ ++ ATS++S+ ++RL+ +T++ E ++ +YLFRTE+GG V VFV KK
Sbjct: 12 FEKMP-RNLSVEVNATSRVSIEPVQKRLSRNTKVEEAEKVSTYLFRTEVGGLVNVFVRKK 70
Query: 707 NIKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQK 886
+ VY+EVSSL SD L LSWGI+RSDSS +P F+ S +ETPFT+
Sbjct: 71 KVNCTVYVEVSSLELPRSDGTLGLSWGIYRSDSSSFLPSHFETST------PVETPFTKN 124
Query: 887 SSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
S G +T + FEAK+ P YLSFLLKS D DS+ +IRSHRKTNFCVP+GF SG P PL
Sbjct: 125 SFGRYTVEFEFEAKEIPCYLSFLLKSPRDNDSSGLDIRSHRKTNFCVPLGFSSGHPTPL 183
>ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Populus trichocarpa]
gi|550345605|gb|ERP64619.1| hypothetical protein
POPTR_0002s22530g [Populus trichocarpa]
Length = 857
Score = 174 bits (442), Expect = 4e-41
Identities = 109/238 (45%), Positives = 141/238 (59%), Gaps = 4/238 (1%)
Frame = +2
Query: 362 MATLLPSVAIRPHCV-NCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVAQKFTKN 538
M TL P +AI P C NCG+ ES K I Y+ K K+ ++F +
Sbjct: 1 MTTLSPLLAITPLCFCNCGSVESSKSI---HYVCKTK------------KSRPTRRFGRM 45
Query: 539 PLRNLNFKLLATSQISVTQTEQRLTTSTE--IRELQETLSYLFRTEIGGHVKVFVGKKNI 712
+ + L A S+++ TEQ + TS+ + +L+E +Y FRTEIGGHVK+ VGK N
Sbjct: 46 DVGKDSRLLFARSRVAAQPTEQMMFTSSNPLVDQLKEVSTYRFRTEIGGHVKISVGKING 105
Query: 713 KYAVYIEVSSL-LRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKS 889
KYAVY+EVSSL L + + L+L WGI+ SDSSC MP+D + A T ETP Q S
Sbjct: 106 KYAVYVEVSSLELGASDNISLMLIWGIYTSDSSCFMPLDSSSHA-----RTRETPLLQNS 160
Query: 890 SGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
F T+L FEAKQTP YLSF LK + S+ EIR+H K+NFCVP+GF SG P PL
Sbjct: 161 CARFATELEFEAKQTPFYLSFFLKPT----SSVVEIRNHNKSNFCVPIGFDSGYPTPL 214
>ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Solanum tuberosum]
Length = 878
Score = 165 bits (418), Expect = 3e-38
Identities = 100/242 (41%), Positives = 134/242 (55%), Gaps = 8/242 (3%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHM--------GRKTEV 517
MAT +A+ ++ G+TES KL+ P+ K +C L K+ + GR +
Sbjct: 1 MATSPIQLAVHSRLLSYGSTESTKLV-PSSSGNRGKIVCSLRKLELEDMNFSGIGRNND- 58
Query: 518 AQKFTKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFV 697
+ P R K L+ S+IS+ + +R+ T YLFRT+IGG VKV V
Sbjct: 59 ----QEAPRRAHRRKALSASRISLVPSAKRVPT------------YLFRTDIGGQVKVLV 102
Query: 698 GKKNIKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPF 877
+ N KY V +EV L + SEL++ WG+FRSD+SC MP+D ++ T+ETPF
Sbjct: 103 ERTNGKYKVLVEVLPLELSYAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPF 162
Query: 878 TQKSSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPA 1057
Q SG T +L FEA P Y+SF +KS L D +SEIRSHR TNF VPVG SG PA
Sbjct: 163 VQGPSGKVTVELDFEASLAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPA 222
Query: 1058 PL 1063
PL
Sbjct: 223 PL 224
>ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|27728147|gb|AAN15318.1|
isoamylase isoform 2 [Solanum tuberosum]
Length = 878
Score = 165 bits (418), Expect = 3e-38
Identities = 100/242 (41%), Positives = 134/242 (55%), Gaps = 8/242 (3%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHM--------GRKTEV 517
MAT +A+ ++ G+TES KL+ P+ K +C L K+ + GR +
Sbjct: 1 MATSPIQLAVHSRLLSYGSTESTKLV-PSSSGNRGKIVCSLRKLELEDMNFSGIGRNND- 58
Query: 518 AQKFTKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFV 697
+ P R K L+ S+IS+ + +R+ T YLFRT+IGG VKV V
Sbjct: 59 ----QEAPRRAHRRKALSASRISLVPSAKRVPT------------YLFRTDIGGQVKVLV 102
Query: 698 GKKNIKYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPF 877
+ N KY V +EV L + SEL++ WG+FRSD+SC MP+D ++ T+ETPF
Sbjct: 103 ERTNGKYKVLVEVLPLELSYAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPF 162
Query: 878 TQKSSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPA 1057
Q SG T +L FEA P Y+SF +KS L D +SEIRSHR TNF VPVG SG PA
Sbjct: 163 VQGPSGKVTVELDFEASLAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPA 222
Query: 1058 PL 1063
PL
Sbjct: 223 PL 224
>ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citrus clementina]
gi|557546394|gb|ESR57372.1| hypothetical protein
CICLE_v10023436mg [Citrus clementina]
Length = 840
Score = 164 bits (415), Expect = 6e-38
Identities = 99/234 (42%), Positives = 124/234 (52%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVAQKFTKNP 541
MATL PS+A RP C NCGA ES +P
Sbjct: 1 MATLAPSLAFRPCCFNCGAVES------------------------------------SP 24
Query: 542 LRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNIKYA 721
L + S V T +TE+ ELQ+ +YLFRT++GG V V VG+KN+ YA
Sbjct: 25 PSKLCAASASASHYPV------FTPTTEVDELQKVSTYLFRTDLGGLVSVSVGEKNVNYA 78
Query: 722 VYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSSGIF 901
V I+VSSL +L+L WG++ SDSS M + Q A+TG+IETPFT S F
Sbjct: 79 VTIDVSSLQTTRGGHKLVLIWGVYTSDSSSYMHLGSQCFTPDAKTGSIETPFTPTSFDTF 138
Query: 902 TTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
+L FEAKQTP YLSFLLKS L S EIR+H FCVPVG +G+P+P+
Sbjct: 139 ALELGFEAKQTPFYLSFLLKSPLRDGSGDFEIRNHLSAKFCVPVGLNAGVPSPM 192
>ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
[Solanum lycopersicum]
Length = 878
Score = 163 bits (413), Expect = 1e-37
Identities = 99/237 (41%), Positives = 130/237 (54%), Gaps = 3/237 (1%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIH---MGRKTEVAQKFT 532
M T +A+ ++ G+TES KL+ PA K +C L K+ M
Sbjct: 1 METSPIQLAVHSRLLSYGSTESTKLV-PASSGNRGKIVCSLRKLELEDMNFSGISRNNDQ 59
Query: 533 KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 712
+ P R K L+ S+IS+ + +R+ T YLFRT+IG VKVFV K N
Sbjct: 60 EAPRRAHRLKALSGSRISLVPSAKRVPT------------YLFRTDIGCQVKVFVEKTNG 107
Query: 713 KYAVYIEVSSLLRLNSDSELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSS 892
KY V +EV L ++ SEL++ WG+FRSD+SC MP+D ++ T+ETPF Q S
Sbjct: 108 KYKVLVEVLPLELSDAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPS 167
Query: 893 GIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
G T +L FE P Y+SF +KS L D +SEIRSHR TNF VPVG SG PAPL
Sbjct: 168 GKVTVELDFEVSLVPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPL 224
>ref|XP_006303226.1| hypothetical protein CARUB_v10012076mg [Capsella rubella]
gi|482571937|gb|EOA36124.1| hypothetical protein
CARUB_v10012076mg [Capsella rubella]
Length = 882
Score = 153 bits (386), Expect = 1e-34
Identities = 91/235 (38%), Positives = 132/235 (56%), Gaps = 1/235 (0%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIGNKAICGLEKIHMGRKTEVAQKFTKNP 541
MA PSV I ++ G T + K P + N+ G E + + RK + T +
Sbjct: 1 MAAWSPSVGIGSFSLHNGITRTWKFPSPRFFTGKNRIKLGSETLIVTRKRFLGDLGT-SA 59
Query: 542 LRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNIKYA 721
L++ + S+ SV +Q T ++ + ++ SYLFRT+ G VKV V KK KY+
Sbjct: 60 LQSYQLSKICASRTSVELQDQVSTRRAQVYDPKKVTSYLFRTKSGALVKVKVEKKREKYS 119
Query: 722 VYIEVSSLLRLNSD-SELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKSSGI 898
+ + VSSL D S L++ WG++RSDSSC +P+DF+NS+ + T T ETPF + SS
Sbjct: 120 IMVHVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSHTHTTETPFVKSSSSE 179
Query: 899 FTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
L F+ K++P +LSF LK D + EI +HR T+FCVPVGF +G P PL
Sbjct: 180 LMLGLEFDGKESPFFLSFHLKLVSGKDPDGQEILTHRDTDFCVPVGFTAGHPLPL 234
>ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
Length = 865
Score = 152 bits (384), Expect = 2e-34
Identities = 88/181 (48%), Positives = 117/181 (64%), Gaps = 2/181 (1%)
Frame = +2
Query: 527 FTKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGH-VKVFVGK 703
F+ NP + KL ATS++S+ +TEQ++ T T +L+ +L+YLFRTE GG VKV V +
Sbjct: 48 FSLNPTSS---KLCATSRLSIEETEQQIGTLTRPEDLKGSLAYLFRTETGGGLVKVHVTR 104
Query: 704 KNIKYAVYIEVSSLLRLNSDSE-LILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFT 880
+N +Y+VYIE+SSL E L+L WG++RSDSSC + +D + A G +P
Sbjct: 105 RNDRYSVYIEISSLDISGGVGEALLLCWGVYRSDSSCFVDLDTIGLSENAAMGMNVSPLV 164
Query: 881 QKSSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAP 1060
Q S G F +L F+AK PLYLSF L SSLD + EIRSHR+TNFC+PVG G P P
Sbjct: 165 QNSDGKFAIELEFDAKHVPLYLSFFLMSSLD---SGLEIRSHRRTNFCMPVGSLPGYPCP 221
Query: 1061 L 1063
L
Sbjct: 222 L 222
>ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
Length = 866
Score = 150 bits (379), Expect = 9e-34
Identities = 89/181 (49%), Positives = 113/181 (62%), Gaps = 2/181 (1%)
Frame = +2
Query: 527 FTKNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGH-VKVFVGK 703
F++NP + KL ATS++S+ +TEQ+L T + L+ + +YLFRTE GG VKV+V K
Sbjct: 49 FSRNPTSS---KLCATSRLSIEETEQQLGTLSRPEYLKGSSAYLFRTETGGGLVKVYVTK 105
Query: 704 KNIKYAVYIEVSSLLRLNSDSE-LILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFT 880
+ +Y VY E+SSL S E L+L WG++RSDSSC + +D A TG +P
Sbjct: 106 RKDRYFVYTEISSLDISGSVGETLLLCWGVYRSDSSCFVDLDTIGLRENAATGMNVSPLV 165
Query: 881 QKSSGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAP 1060
Q S G F +L F+AK PLYLSF L SSLD EIRSHR TNFCVPVG G P P
Sbjct: 166 QNSDGNFAVELEFDAKHVPLYLSFFLMSSLDA---GMEIRSHRGTNFCVPVGLLPGYPGP 222
Query: 1061 L 1063
L
Sbjct: 223 L 223
>ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] gi|42571321|ref|NP_973751.1|
Isoamylase 2 [Arabidopsis thaliana]
gi|251764780|sp|Q8L735.2|ISOA2_ARATH RecName:
Full=Isoamylase 2, chloroplastic; Short=AtISA2; AltName:
Full=Protein DEBRANCHING ENZYME 1; Flags: Precursor
gi|3850573|gb|AAC72113.1| Similar to gi|1652733 glycogen
operon protein GlgX from Synechocystis sp. genome
gb|D90908. ESTs gb|H36690, gb|AA712462, gb|AA651230 and
gb|N95932 come from this gene [Arabidopsis thaliana]
gi|332189436|gb|AEE27557.1| Isoamylase 2 [Arabidopsis
thaliana] gi|332189437|gb|AEE27558.1| Isoamylase 2
[Arabidopsis thaliana]
Length = 882
Score = 150 bits (378), Expect = 1e-33
Identities = 92/238 (38%), Positives = 132/238 (55%), Gaps = 4/238 (1%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIG---NKAICGLEKIHMGRKTEVAQKFT 532
MA PSV I C+N G T + K FP+ + NK G E + RK + T
Sbjct: 1 MAAWSPSVGIGSCCLNNGITRTWK--FPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVT 58
Query: 533 KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 712
+ L++ F + S+ S+ E + E +L++ SY FRT+ G VKV V KK
Sbjct: 59 -SALQSYQFSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKRE 117
Query: 713 KYAVYIEVSSLLRLNSD-SELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKS 889
KY++ + VSSL D S L++ WG++RSDSSC +P+DF+NS+ ++T T ET F + S
Sbjct: 118 KYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSS 177
Query: 890 SGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
L F+ K++P YLSF LK D + E+ +HR T+FC+PVGF +G P PL
Sbjct: 178 LSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPL 235
>gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]
Length = 882
Score = 149 bits (377), Expect = 1e-33
Identities = 92/238 (38%), Positives = 132/238 (55%), Gaps = 4/238 (1%)
Frame = +2
Query: 362 MATLLPSVAIRPHCVNCGATESPKLIFPAQYIIG---NKAICGLEKIHMGRKTEVAQKFT 532
MA PSV I C+N G T + K FP+ + NK G E + RK + T
Sbjct: 1 MAAWSPSVGIGSCCLNNGITRTWK--FPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVT 58
Query: 533 KNPLRNLNFKLLATSQISVTQTEQRLTTSTEIRELQETLSYLFRTEIGGHVKVFVGKKNI 712
+ L++ F + S+ S+ E + E +L++ SY FRT+ G VKV V KK
Sbjct: 59 -SALQSYQFSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKRE 117
Query: 713 KYAVYIEVSSLLRLNSD-SELILSWGIFRSDSSCLMPVDFQNSALGARTGTIETPFTQKS 889
KY++ + VSSL D S L++ WG++RSDSSC +P+DF+NS+ ++T T ET F + S
Sbjct: 118 KYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSS 177
Query: 890 SGIFTTDLVFEAKQTPLYLSFLLKSSLDVDSNSSEIRSHRKTNFCVPVGFGSGIPAPL 1063
L F+ K++P YLSF LK D + E+ +HR T+FC+PVGF +G P PL
Sbjct: 178 LSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGREMLTHRDTDFCIPVGFTAGHPLPL 235