BLASTX nr result
ID: Ophiopogon27_contig00050092
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon27_contig00050092
(600 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|PKK72470.1| hypothetical protein RhiirC2_742596 [Rhizophagus ... 254 7e-84
gb|EXX70618.1| Gcn4p [Rhizophagus irregularis DAOM 197198w] >gi|... 253 3e-83
gb|PKC06099.1| hypothetical protein RhiirA5_293643 [Rhizophagus ... 106 2e-25
dbj|GBC32348.1| amino acid starvation-responsive transcription f... 106 7e-25
gb|PKC65772.1| hypothetical protein RhiirA1_420097 [Rhizophagus ... 106 2e-24
gb|EXX51626.1| Gcn4p [Rhizophagus irregularis DAOM 197198w] >gi|... 106 2e-24
emb|CDS05053.1| hypothetical protein LRAMOSA07582 [Lichtheimia r... 87 6e-17
emb|CDH51504.1| predicted protein [Lichtheimia corymbifera JMRC:... 87 6e-17
emb|CDH49173.1| predicted protein [Lichtheimia corymbifera JMRC:... 84 2e-16
emb|CDH49172.1| predicted protein [Lichtheimia corymbifera JMRC:... 84 5e-16
ref|XP_018285958.1| basic-leucine zipper transcription factor [P... 84 5e-16
emb|CEP07474.1| hypothetical protein [Parasitella parasitica] 84 6e-16
dbj|GAN06368.1| hypothetical protein MAM1_0121d05850 [Mucor ambi... 84 7e-16
emb|CDS08135.1| hypothetical protein LRAMOSA02083 [Lichtheimia r... 83 9e-16
gb|OBZ84535.1| hypothetical protein A0J61_07415 [Choanephora cuc... 82 2e-15
gb|ORZ23493.1| hypothetical protein BCR42DRAFT_404910 [Absidia r... 83 2e-15
gb|OBZ90650.1| G-box-binding factor 1 [Choanephora cucurbitarum] 82 2e-15
emb|CEJ05427.1| hypothetical protein RMCBS344292_19368 [Rhizopus... 78 2e-15
gb|OAD08513.1| basic-leucine zipper transcription factor [Mucor ... 82 2e-15
gb|ORE15122.1| hypothetical protein BCV71DRAFT_228862 [Rhizopus ... 81 2e-15
>gb|PKK72470.1| hypothetical protein RhiirC2_742596 [Rhizophagus irregularis]
gb|PKY39743.1| hypothetical protein RhiirA4_393862 [Rhizophagus irregularis]
Length = 159
Score = 254 bits (649), Expect = 7e-84
Identities = 137/159 (86%), Positives = 139/159 (87%)
Frame = +1
Query: 88 MSNKVQSEKVKPNGTXXXXXXXXXXIGPSCSVADSTSATMAALTSEQTKXXXXXXXXXRK 267
MSNKVQSEKVKPNGT IGPSCSVADSTSATMAALTSEQTK RK
Sbjct: 1 MSNKVQSEKVKPNGTSYSSTQDSSSIGPSCSVADSTSATMAALTSEQTKTSTNNSSSSRK 60
Query: 268 KAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRL 447
KAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRL
Sbjct: 61 KAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRL 120
Query: 448 TVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQGIVNRS 564
TVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQGI+ +S
Sbjct: 121 TVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQGIIKKS 159
>gb|EXX70618.1| Gcn4p [Rhizophagus irregularis DAOM 197198w]
dbj|GBC15176.1| amino acid starvation-responsive transcription factor gcn4
[Rhizophagus irregularis DAOM 181602]
gb|PKC14622.1| hypothetical protein RhiirA5_350046 [Rhizophagus irregularis]
gb|PKC70837.1| hypothetical protein RhiirA1_413916 [Rhizophagus irregularis]
gb|PKY19846.1| hypothetical protein RhiirB3_407414 [Rhizophagus irregularis]
gb|POG81231.1| hypothetical protein GLOIN_2v1508776 [Rhizophagus irregularis DAOM
181602=DAOM 197198]
Length = 159
Score = 253 bits (645), Expect = 3e-83
Identities = 136/159 (85%), Positives = 139/159 (87%)
Frame = +1
Query: 88 MSNKVQSEKVKPNGTXXXXXXXXXXIGPSCSVADSTSATMAALTSEQTKXXXXXXXXXRK 267
MSNKVQSEKVKPNGT IGPSCSVADSTSATMAALTSEQ+K RK
Sbjct: 1 MSNKVQSEKVKPNGTSYSSTQDSSSIGPSCSVADSTSATMAALTSEQSKTSTNNSSSSRK 60
Query: 268 KAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRL 447
KAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRL
Sbjct: 61 KAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRL 120
Query: 448 TVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQGIVNRS 564
TVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQGI+ +S
Sbjct: 121 TVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQGIIKKS 159
>gb|PKC06099.1| hypothetical protein RhiirA5_293643 [Rhizophagus irregularis]
gb|PKK66467.1| hypothetical protein RhiirC2_664180 [Rhizophagus irregularis]
gb|PKY47856.1| hypothetical protein RhiirA4_321674 [Rhizophagus irregularis]
gb|POG59784.1| hypothetical protein GLOIN_2v1467059 [Rhizophagus irregularis DAOM
181602=DAOM 197198]
Length = 179
Score = 106 bits (265), Expect = 2e-25
Identities = 58/122 (47%), Positives = 85/122 (69%)
Frame = +1
Query: 190 STSATMAALTSEQTKXXXXXXXXXRKKAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQR 369
S++ T +A TS T RK++ + KDP + ++L KRAKNT+AARRSR R
Sbjct: 55 SSNTTSSASTSPLTTSLRTRN---RKRSANEIDKDPIIVADELAMKRAKNTDAARRSRLR 111
Query: 370 RVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQG 549
+V++MESLEK+V L EN +L+TR+ VLE EKK LE+KN +KD+ +K++E++LTEA+
Sbjct: 112 KVKRMESLEKEVTILKNENIDLQTRIAVLESEKKGLEDKNAEKDARVKMMEQRLTEAHDR 171
Query: 550 IV 555
+V
Sbjct: 172 LV 173
>dbj|GBC32348.1| amino acid starvation-responsive transcription factor gcn4
[Rhizophagus irregularis DAOM 181602]
Length = 235
Score = 106 bits (265), Expect = 7e-25
Identities = 58/122 (47%), Positives = 85/122 (69%)
Frame = +1
Query: 190 STSATMAALTSEQTKXXXXXXXXXRKKAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQR 369
S++ T +A TS T RK++ + KDP + ++L KRAKNT+AARRSR R
Sbjct: 111 SSNTTSSASTSPLTTSLRTRN---RKRSANEIDKDPIIVADELAMKRAKNTDAARRSRLR 167
Query: 370 RVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQG 549
+V++MESLEK+V L EN +L+TR+ VLE EKK LE+KN +KD+ +K++E++LTEA+
Sbjct: 168 KVKRMESLEKEVTILKNENIDLQTRIAVLESEKKGLEDKNAEKDARVKMMEQRLTEAHDR 227
Query: 550 IV 555
+V
Sbjct: 228 LV 229
>gb|PKC65772.1| hypothetical protein RhiirA1_420097 [Rhizophagus irregularis]
gb|PKY29809.1| hypothetical protein RhiirB3_418297 [Rhizophagus irregularis]
Length = 297
Score = 106 bits (265), Expect = 2e-24
Identities = 58/122 (47%), Positives = 85/122 (69%)
Frame = +1
Query: 190 STSATMAALTSEQTKXXXXXXXXXRKKAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQR 369
S++ T +A TS T RK++ + KDP + ++L KRAKNT+AARRSR R
Sbjct: 173 SSNTTSSASTSPLTTSLRTRN---RKRSANEIDKDPIIVADELAMKRAKNTDAARRSRLR 229
Query: 370 RVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQG 549
+V++MESLEK+V L EN +L+TR+ VLE EKK LE+KN +KD+ +K++E++LTEA+
Sbjct: 230 KVKRMESLEKEVTILKNENIDLQTRIAVLESEKKGLEDKNAEKDARVKMMEQRLTEAHDR 289
Query: 550 IV 555
+V
Sbjct: 290 LV 291
>gb|EXX51626.1| Gcn4p [Rhizophagus irregularis DAOM 197198w]
dbj|GBC32347.1| amino acid starvation-responsive transcription factor gcn4
[Rhizophagus irregularis DAOM 181602]
Length = 298
Score = 106 bits (265), Expect = 2e-24
Identities = 58/122 (47%), Positives = 85/122 (69%)
Frame = +1
Query: 190 STSATMAALTSEQTKXXXXXXXXXRKKAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQR 369
S++ T +A TS T RK++ + KDP + ++L KRAKNT+AARRSR R
Sbjct: 174 SSNTTSSASTSPLTTSLRTRN---RKRSANEIDKDPIIVADELAMKRAKNTDAARRSRLR 230
Query: 370 RVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQG 549
+V++MESLEK+V L EN +L+TR+ VLE EKK LE+KN +KD+ +K++E++LTEA+
Sbjct: 231 KVKRMESLEKEVTILKNENIDLQTRIAVLESEKKGLEDKNAEKDARVKMMEQRLTEAHDR 290
Query: 550 IV 555
+V
Sbjct: 291 LV 292
>emb|CDS05053.1| hypothetical protein LRAMOSA07582 [Lichtheimia ramosa]
Length = 278
Score = 86.7 bits (213), Expect = 6e-17
Identities = 43/84 (51%), Positives = 61/84 (72%)
Frame = +1
Query: 310 EDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKN 489
ED++ KR +NT+AARRSR +++ KME+LEK+V EL ENT L TR+ VLE EK LE K+
Sbjct: 192 EDIVLKRQRNTDAARRSRLKKLLKMEALEKRVNELEGENTRLTTRVAVLESEKSGLENKD 251
Query: 490 LDKDSMIKILERQLTEAYQGIVNR 561
D I++LE QL EA++ + ++
Sbjct: 252 KDLQERIRVLEEQLAEAHKALTSK 275
>emb|CDH51504.1| predicted protein [Lichtheimia corymbifera JMRC:FSU:9682]
Length = 279
Score = 86.7 bits (213), Expect = 6e-17
Identities = 43/84 (51%), Positives = 61/84 (72%)
Frame = +1
Query: 310 EDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKN 489
ED++ KR +NT+AARRSR +++ KME+LEK+V EL ENT L TR+ VLE EK LE K+
Sbjct: 193 EDIVLKRQRNTDAARRSRLKKLLKMEALEKRVNELEGENTRLTTRVAVLESEKSGLENKD 252
Query: 490 LDKDSMIKILERQLTEAYQGIVNR 561
D I++LE QL EA++ + ++
Sbjct: 253 KDLQERIRVLEEQLAEAHKALTSK 276
>emb|CDH49173.1| predicted protein [Lichtheimia corymbifera JMRC:FSU:9682]
Length = 215
Score = 84.0 bits (206), Expect = 2e-16
Identities = 46/124 (37%), Positives = 72/124 (58%)
Frame = +1
Query: 190 STSATMAALTSEQTKXXXXXXXXXRKKAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQR 369
+T+ M +T + ++K + +D +E+++RKR +NT+AARRSR +
Sbjct: 91 TTTTIMPTITHGNSSNNNPMSSCMKRKTSLS--QDSSEESEEVVRKRQRNTDAARRSRMK 148
Query: 370 RVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQG 549
++ KME+LE +V EL +N L TR+ VLE EK LE K+ D IK LE QL EA++
Sbjct: 149 KMLKMEALENRVNELENDNARLTTRVAVLESEKNGLETKDNDLQERIKALEEQLAEAHKA 208
Query: 550 IVNR 561
+ +
Sbjct: 209 LTGK 212
>emb|CDH49172.1| predicted protein [Lichtheimia corymbifera JMRC:FSU:9682]
Length = 262
Score = 84.0 bits (206), Expect = 5e-16
Identities = 46/124 (37%), Positives = 72/124 (58%)
Frame = +1
Query: 190 STSATMAALTSEQTKXXXXXXXXXRKKAFIKFVKDPQTITEDLIRKRAKNTEAARRSRQR 369
+T+ M +T + ++K + +D +E+++RKR +NT+AARRSR +
Sbjct: 138 TTTTIMPTITHGNSSNNNPMSSCMKRKTSLS--QDSSEESEEVVRKRQRNTDAARRSRMK 195
Query: 370 RVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKNLDKDSMIKILERQLTEAYQG 549
++ KME+LE +V EL +N L TR+ VLE EK LE K+ D IK LE QL EA++
Sbjct: 196 KMLKMEALENRVNELENDNARLTTRVAVLESEKNGLETKDNDLQERIKALEEQLAEAHKA 255
Query: 550 IVNR 561
+ +
Sbjct: 256 LTGK 259
>ref|XP_018285958.1| basic-leucine zipper transcription factor [Phycomyces blakesleeanus
NRRL 1555(-)]
gb|OAD67918.1| basic-leucine zipper transcription factor [Phycomyces blakesleeanus
NRRL 1555(-)]
Length = 267
Score = 84.0 bits (206), Expect = 5e-16
Identities = 40/83 (48%), Positives = 61/83 (73%)
Frame = +1
Query: 310 EDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKN 489
+D+ KR KNT+AARRSR +++++ME LE++V+EL +NT L TR+ VLE EK LE K+
Sbjct: 183 DDIAVKRQKNTDAARRSRLKKLKRMECLEERVSELETDNTRLTTRIAVLESEKSGLESKD 242
Query: 490 LDKDSMIKILERQLTEAYQGIVN 558
+ ++ILE+QL EA++ + N
Sbjct: 243 RGLEERVRILEQQLAEAHRALTN 265
>emb|CEP07474.1| hypothetical protein [Parasitella parasitica]
Length = 262
Score = 83.6 bits (205), Expect = 6e-16
Identities = 42/89 (47%), Positives = 64/89 (71%)
Frame = +1
Query: 298 QTITEDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSL 477
Q +++ +KR KNT+AARRSR +++ KME+LEKQVAEL +N+ L TR+ VLE EK +L
Sbjct: 173 QQDSDEAAQKRQKNTDAARRSRLKKIIKMETLEKQVAELESDNSRLTTRVAVLESEKSAL 232
Query: 478 EEKNLDKDSMIKILERQLTEAYQGIVNRS 564
K+ + I++LE QL EA++ + ++S
Sbjct: 233 LSKDRGLEDRIRVLEAQLAEAHRALTSKS 261
>dbj|GAN06368.1| hypothetical protein MAM1_0121d05850 [Mucor ambiguus]
Length = 263
Score = 83.6 bits (205), Expect = 7e-16
Identities = 41/89 (46%), Positives = 64/89 (71%)
Frame = +1
Query: 298 QTITEDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSL 477
Q +++ +KR KNT+AARRSR +++ KME+LEKQV EL +N+ L TR+ VLE EK +L
Sbjct: 174 QQDSDEAAQKRQKNTDAARRSRLKKIIKMETLEKQVTELESDNSRLTTRVAVLESEKSAL 233
Query: 478 EEKNLDKDSMIKILERQLTEAYQGIVNRS 564
K++ + I++LE QL EA++ + ++S
Sbjct: 234 ISKDIGLEDRIRVLEAQLAEAHRALTSKS 262
>emb|CDS08135.1| hypothetical protein LRAMOSA02083 [Lichtheimia ramosa]
Length = 243
Score = 82.8 bits (203), Expect = 9e-16
Identities = 42/84 (50%), Positives = 59/84 (70%)
Frame = +1
Query: 310 EDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKN 489
E++IRKR +NT+AARRSR +++ KME+LE +V EL +N L TR+ VLE EK LE K+
Sbjct: 157 EEVIRKRQRNTDAARRSRMKKMLKMEALENRVNELENDNARLTTRVAVLESEKSGLETKD 216
Query: 490 LDKDSMIKILERQLTEAYQGIVNR 561
D IK LE QL EA++ + ++
Sbjct: 217 NDLQERIKALEEQLAEAHKALTSK 240
>gb|OBZ84535.1| hypothetical protein A0J61_07415 [Choanephora cucurbitarum]
Length = 237
Score = 82.0 bits (201), Expect = 2e-15
Identities = 41/88 (46%), Positives = 60/88 (68%)
Frame = +1
Query: 289 KDPQTITEDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLEIEK 468
K +++ +KR KNT+AARRSR +++ KME+LEKQV EL +N L TR+ VLE EK
Sbjct: 146 KSENVESDEAAQKRQKNTDAARRSRLKKIIKMETLEKQVTELESDNARLTTRVAVLESEK 205
Query: 469 KSLEEKNLDKDSMIKILERQLTEAYQGI 552
+L K+ D + I++LE QL EA++ +
Sbjct: 206 SALVSKDKDLEERIRVLEAQLAEAHRAL 233
>gb|ORZ23493.1| hypothetical protein BCR42DRAFT_404910 [Absidia repens]
Length = 313
Score = 83.2 bits (204), Expect = 2e-15
Identities = 43/94 (45%), Positives = 64/94 (68%)
Frame = +1
Query: 280 KFVKDPQTITEDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLE 459
K+V + ++ +D+ KR KNT+AARRSR +++ KME+LE +V EL +NT L TR+ VLE
Sbjct: 221 KYVDEKES--DDIAMKRQKNTDAARRSRLKKLVKMEALEARVVELETDNTRLSTRIAVLE 278
Query: 460 IEKKSLEEKNLDKDSMIKILERQLTEAYQGIVNR 561
EK LE K+ + I+ LE QL EA++ + +R
Sbjct: 279 SEKSGLESKDKGLEERIRTLEAQLAEAHKALTSR 312
>gb|OBZ90650.1| G-box-binding factor 1 [Choanephora cucurbitarum]
Length = 225
Score = 81.6 bits (200), Expect = 2e-15
Identities = 40/84 (47%), Positives = 59/84 (70%)
Frame = +1
Query: 310 EDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKN 489
+++ KR KNT+AARRSR +++ KMESLEK+V+EL +N +L TR+ VLE EK L K+
Sbjct: 142 DEIALKRQKNTDAARRSRLKKLMKMESLEKRVSELESDNNQLNTRIAVLESEKSGLTSKD 201
Query: 490 LDKDSMIKILERQLTEAYQGIVNR 561
I++LE QL EA++ + +R
Sbjct: 202 QSLQERIRVLEAQLAEAHRALTSR 225
>emb|CEJ05427.1| hypothetical protein RMCBS344292_19368 [Rhizopus microsporus]
emb|CEI97528.1| hypothetical protein RMCBS344292_11659 [Rhizopus microsporus]
Length = 86
Score = 77.8 bits (190), Expect = 2e-15
Identities = 41/79 (51%), Positives = 57/79 (72%)
Frame = +1
Query: 325 KRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKNLDKDS 504
KR KNT+AARRSR R+V+KME+LEK+VAEL N L R+ VL+ EK +L+ K ++
Sbjct: 7 KRQKNTDAARRSRLRKVQKMETLEKKVAELEKINASLLMRVAVLDSEKTNLKAKESSYEN 66
Query: 505 MIKILERQLTEAYQGIVNR 561
IK+LE QL EA++ + +R
Sbjct: 67 RIKVLEGQLAEAHKALSSR 85
>gb|OAD08513.1| basic-leucine zipper transcription factor [Mucor circinelloides f.
lusitanicus CBS 277.49]
Length = 258
Score = 82.0 bits (201), Expect = 2e-15
Identities = 41/89 (46%), Positives = 63/89 (70%)
Frame = +1
Query: 298 QTITEDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSL 477
Q +++ +KR KNT+AARRSR +++ KME+LEKQV EL +N+ L TR+ VLE EK +L
Sbjct: 169 QQDSDEAAQKRQKNTDAARRSRLKKIIKMETLEKQVTELESDNSRLTTRVAVLESEKSAL 228
Query: 478 EEKNLDKDSMIKILERQLTEAYQGIVNRS 564
K+ + I++LE QL EA++ + ++S
Sbjct: 229 ISKDKGLEDRIRVLEAQLAEAHRALTSKS 257
>gb|ORE15122.1| hypothetical protein BCV71DRAFT_228862 [Rhizopus microsporus]
Length = 223
Score = 81.3 bits (199), Expect = 2e-15
Identities = 40/83 (48%), Positives = 59/83 (71%)
Frame = +1
Query: 310 EDLIRKRAKNTEAARRSRQRRVEKMESLEKQVAELTIENTELKTRLTVLEIEKKSLEEKN 489
+++ KR KNT+AARRSR +++ KME+LEKQV EL EN+ L TR+ VLE EK +L K
Sbjct: 138 DEIALKRQKNTDAARRSRLKKILKMENLEKQVKELECENSRLTTRVAVLESEKSALASKE 197
Query: 490 LDKDSMIKILERQLTEAYQGIVN 558
+ I++LE QL+EA++ + +
Sbjct: 198 KSLEERIRVLESQLSEAHRALTS 220