BLASTX nr result
ID: Ophiopogon27_contig00043834
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon27_contig00043834
(576 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|GBC13413.1| hypothetical protein RIR_0214300 [Rhizophagus ir... 182 5e-55
gb|PKC56308.1| hypothetical protein RhiirA1_542170 [Rhizophagus ... 149 5e-47
gb|POG81184.1| hypothetical protein GLOIN_2v1511347 [Rhizophagus... 153 1e-43
gb|PKC02837.1| hypothetical protein RhiirA5_363752, partial [Rhi... 149 2e-41
gb|PKY20829.1| hypothetical protein RhiirB3_498916 [Rhizophagus ... 147 2e-41
gb|PKC66944.1| hypothetical protein RhiirA1_510905 [Rhizophagus ... 145 1e-40
gb|PKK72890.1| hypothetical protein RhiirC2_824960 [Rhizophagus ... 145 1e-40
gb|EXX75764.1| hypothetical protein RirG_039080 [Rhizophagus irr... 145 1e-40
dbj|GBC26156.1| hypothetical protein RIR_1248000 [Rhizophagus ir... 149 1e-40
gb|PKY33494.1| hypothetical protein RhiirB3_532509 [Rhizophagus ... 149 8e-40
gb|PKK61712.1| hypothetical protein RhiirC2_856094 [Rhizophagus ... 150 9e-40
gb|PKY47288.1| hypothetical protein RhiirA4_462419 [Rhizophagus ... 147 2e-39
gb|PKK73569.1| hypothetical protein RhiirC2_333131 [Rhizophagus ... 141 3e-39
gb|EXX57191.1| hypothetical protein RirG_209410 [Rhizophagus irr... 141 6e-39
gb|PKY40192.1| hypothetical protein RhiirA4_453508 [Rhizophagus ... 142 6e-39
gb|POG75728.1| hypothetical protein GLOIN_2v1561751 [Rhizophagus... 141 9e-39
gb|PKY56483.1| hypothetical protein RhiirA4_549253 [Rhizophagus ... 139 1e-38
dbj|GBC43469.1| f-box/lrr-repeat protein 7-like [Rhizophagus irr... 143 6e-38
gb|POG60228.1| hypothetical protein GLOIN_2v1788170 [Rhizophagus... 132 2e-35
gb|PKC53478.1| hypothetical protein RhiirA1_479208 [Rhizophagus ... 132 2e-35
>dbj|GBC13413.1| hypothetical protein RIR_0214300 [Rhizophagus irregularis DAOM
181602]
Length = 201
Score = 182 bits (462), Expect = 5e-55
Identities = 85/102 (83%), Positives = 94/102 (92%), Gaps = 1/102 (0%)
Frame = +3
Query: 66 YITCINCHCQKTHLSRYIHSFT-KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGY 242
YITCINCH Q+TH SRYIHSF +D+SGEDATG+VDYAIKSLEDLLCIT+GKPRNIKIGY
Sbjct: 28 YITCINCHRQETHFSRYIHSFAERDVSGEDATGRVDYAIKSLEDLLCITEGKPRNIKIGY 87
Query: 243 AQNLAQLESAFQTNKKKQTADQAFNEEYFDYLYGIVITGTEW 368
AQNLAQLESAFQ NKKK+TADQAF +YFDY+YG+V TGTEW
Sbjct: 88 AQNLAQLESAFQANKKKRTADQAFGNDYFDYIYGVVTTGTEW 129
>gb|PKC56308.1| hypothetical protein RhiirA1_542170 [Rhizophagus irregularis]
Length = 346
Score = 149 bits (377), Expect(3) = 5e-47
Identities = 70/79 (88%), Positives = 76/79 (96%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KD+SGEDATG+VDYAIKSLEDLLCIT+GKPRNIKIGYAQNLAQLESAFQTNKKK+TADQA
Sbjct: 196 KDVSGEDATGRVDYAIKSLEDLLCITEGKPRNIKIGYAQNLAQLESAFQTNKKKRTADQA 255
Query: 312 FNEEYFDYLYGIVITGTEW 368
F +YFDY+YGIV TGTEW
Sbjct: 256 FGNDYFDYIYGIVTTGTEW 274
Score = 46.2 bits (108), Expect(3) = 5e-47
Identities = 29/54 (53%), Positives = 33/54 (61%), Gaps = 2/54 (3%)
Frame = +2
Query: 359 DGV--VSGSEYQINLTKSTIEDNLELL*SNMXXXXXXXXXXXXDHVTVSVDSSP 514
DG+ SGSEYQINLTKS ++DN ELL SN+ D VSVDSSP
Sbjct: 282 DGIYSTSGSEYQINLTKSAVKDNPELLRSNVKRVIGIIVGLLKDR--VSVDSSP 333
Score = 40.8 bits (94), Expect(3) = 5e-47
Identities = 21/38 (55%), Positives = 22/38 (57%)
Frame = +1
Query: 22 VTDANEATRCEFXXXXXXXXXXXXXXXTSQDIFIVLQK 135
+TDANE TRCEF TSQDIFIVLQK
Sbjct: 159 MTDANEVTRCEFISPILHASIAIAKNLTSQDIFIVLQK 196
>gb|POG81184.1| hypothetical protein GLOIN_2v1511347 [Rhizophagus irregularis DAOM
181602=DAOM 197198]
Length = 201
Score = 153 bits (387), Expect = 1e-43
Identities = 73/79 (92%), Positives = 78/79 (98%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KDISGEDATG+VDYAIKSLE+LLCIT+GKPRNIKIGYAQNLAQLESAFQTNKKK+TADQA
Sbjct: 51 KDISGEDATGRVDYAIKSLEELLCITEGKPRNIKIGYAQNLAQLESAFQTNKKKRTADQA 110
Query: 312 FNEEYFDYLYGIVITGTEW 368
FNE+YFDYLYGIV TGTEW
Sbjct: 111 FNEDYFDYLYGIVTTGTEW 129
>gb|PKC02837.1| hypothetical protein RhiirA5_363752, partial [Rhizophagus
irregularis]
Length = 257
Score = 149 bits (377), Expect = 2e-41
Identities = 70/79 (88%), Positives = 76/79 (96%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KD+SGEDATG+VDYAIKSLEDLLCIT+GKPRNIKIGYAQNLAQLESAFQTNKKK+TADQA
Sbjct: 127 KDVSGEDATGRVDYAIKSLEDLLCITEGKPRNIKIGYAQNLAQLESAFQTNKKKRTADQA 186
Query: 312 FNEEYFDYLYGIVITGTEW 368
F +YFDY+YGIV TGTEW
Sbjct: 187 FGNDYFDYIYGIVTTGTEW 205
>gb|PKY20829.1| hypothetical protein RhiirB3_498916 [Rhizophagus irregularis]
Length = 184
Score = 147 bits (370), Expect = 2e-41
Identities = 69/79 (87%), Positives = 75/79 (94%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KDISGEDATG++DYAIKSLEDLLCI +GKPRNIKIGYAQNLAQLESAFQTNKKK+TADQA
Sbjct: 38 KDISGEDATGRLDYAIKSLEDLLCIAEGKPRNIKIGYAQNLAQLESAFQTNKKKRTADQA 97
Query: 312 FNEEYFDYLYGIVITGTEW 368
F +YFDY+YGIV TGTEW
Sbjct: 98 FGNDYFDYIYGIVTTGTEW 116
>gb|PKC66944.1| hypothetical protein RhiirA1_510905 [Rhizophagus irregularis]
Length = 184
Score = 145 bits (366), Expect = 1e-40
Identities = 68/79 (86%), Positives = 75/79 (94%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KDISGEDATG++DYAIKSLEDLLCI +GKPRNIKIGYAQNLAQLESAFQTNKKK+TAD+A
Sbjct: 38 KDISGEDATGRLDYAIKSLEDLLCIAEGKPRNIKIGYAQNLAQLESAFQTNKKKRTADRA 97
Query: 312 FNEEYFDYLYGIVITGTEW 368
F +YFDY+YGIV TGTEW
Sbjct: 98 FGNDYFDYIYGIVTTGTEW 116
>gb|PKK72890.1| hypothetical protein RhiirC2_824960 [Rhizophagus irregularis]
Length = 188
Score = 145 bits (366), Expect = 1e-40
Identities = 68/79 (86%), Positives = 75/79 (94%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KDISGEDATG++DYAIKSLEDLLCI +GKPRNIKIGYAQNLAQLESAFQTNKKK+TAD+A
Sbjct: 38 KDISGEDATGRLDYAIKSLEDLLCIAEGKPRNIKIGYAQNLAQLESAFQTNKKKRTADRA 97
Query: 312 FNEEYFDYLYGIVITGTEW 368
F +YFDY+YGIV TGTEW
Sbjct: 98 FGNDYFDYIYGIVTTGTEW 116
>gb|EXX75764.1| hypothetical protein RirG_039080 [Rhizophagus irregularis DAOM
197198w]
Length = 188
Score = 145 bits (366), Expect = 1e-40
Identities = 68/79 (86%), Positives = 75/79 (94%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KDISGEDATG++DYAIKSLEDLLCI +GKPRNIKIGYAQNLAQLESAFQTNKKK+TAD+A
Sbjct: 38 KDISGEDATGRLDYAIKSLEDLLCIAEGKPRNIKIGYAQNLAQLESAFQTNKKKRTADRA 97
Query: 312 FNEEYFDYLYGIVITGTEW 368
F +YFDY+YGIV TGTEW
Sbjct: 98 FGNDYFDYIYGIVTTGTEW 116
>dbj|GBC26156.1| hypothetical protein RIR_1248000 [Rhizophagus irregularis DAOM
181602]
Length = 582
Score = 149 bits (377), Expect(3) = 1e-40
Identities = 70/79 (88%), Positives = 76/79 (96%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KD+SGEDATG+VDYAIKSLEDLLCIT+GKPRNIKIGYAQNLAQLESAFQTNKKK+TADQA
Sbjct: 168 KDVSGEDATGRVDYAIKSLEDLLCITEGKPRNIKIGYAQNLAQLESAFQTNKKKRTADQA 227
Query: 312 FNEEYFDYLYGIVITGTEW 368
F +YFDY+YGIV TGTEW
Sbjct: 228 FGNDYFDYIYGIVTTGTEW 246
Score = 40.8 bits (94), Expect(3) = 1e-40
Identities = 21/31 (67%), Positives = 25/31 (80%), Gaps = 2/31 (6%)
Frame = +2
Query: 359 DGV--VSGSEYQINLTKSTIEDNLELL*SNM 445
DG+ SGSEYQINLTKS ++DN ELL SN+
Sbjct: 254 DGIYSTSGSEYQINLTKSAVKDNPELLRSNV 284
Score = 24.6 bits (52), Expect(3) = 1e-40
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +1
Query: 103 TSQDIFIVLQK 135
TSQDIFIVLQK
Sbjct: 158 TSQDIFIVLQK 168
>gb|PKY33494.1| hypothetical protein RhiirB3_532509 [Rhizophagus irregularis]
Length = 429
Score = 149 bits (377), Expect = 8e-40
Identities = 70/79 (88%), Positives = 76/79 (96%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KD+SGEDATG+VDYAIKSLEDLLCIT+GKPRNIKIGYAQNLAQLESAFQTNKKK+TADQA
Sbjct: 126 KDVSGEDATGRVDYAIKSLEDLLCITEGKPRNIKIGYAQNLAQLESAFQTNKKKRTADQA 185
Query: 312 FNEEYFDYLYGIVITGTEW 368
F +YFDY+YGIV TGTEW
Sbjct: 186 FGNDYFDYIYGIVTTGTEW 204
>gb|PKK61712.1| hypothetical protein RhiirC2_856094 [Rhizophagus irregularis]
Length = 473
Score = 150 bits (379), Expect = 9e-40
Identities = 82/128 (64%), Positives = 95/128 (74%), Gaps = 6/128 (4%)
Frame = +3
Query: 3 LSNVET------SDRCQRGNAL*IYFSYITCINCHCQKTHLSRYIHSFTKDISGEDATGQ 164
LSNVET + RC+ +A+ ++ S N Q + KD+SGEDATG+
Sbjct: 144 LSNVETMTDANEATRCEFISAI-LHASIAIAKNLTSQDIFIV-----LQKDVSGEDATGR 197
Query: 165 VDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQAFNEEYFDYLYG 344
VDYAIKSLEDLLCIT+GKPRNIKIGYAQNLAQLESAFQTNKKK+TADQAF +YFDY+YG
Sbjct: 198 VDYAIKSLEDLLCITEGKPRNIKIGYAQNLAQLESAFQTNKKKRTADQAFGNDYFDYIYG 257
Query: 345 IVITGTEW 368
IV TGTEW
Sbjct: 258 IVTTGTEW 265
>gb|PKY47288.1| hypothetical protein RhiirA4_462419 [Rhizophagus irregularis]
Length = 383
Score = 147 bits (372), Expect = 2e-39
Identities = 70/79 (88%), Positives = 75/79 (94%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KDISGEDATG++DYAIKSLEDLLCIT+GKPRNIKIGYAQNLAQLESAFQTNKKK TADQA
Sbjct: 182 KDISGEDATGRLDYAIKSLEDLLCITEGKPRNIKIGYAQNLAQLESAFQTNKKKWTADQA 241
Query: 312 FNEEYFDYLYGIVITGTEW 368
F +YFDY+YGIV TGTEW
Sbjct: 242 FGNDYFDYIYGIVTTGTEW 260
>gb|PKK73569.1| hypothetical protein RhiirC2_333131 [Rhizophagus irregularis]
Length = 180
Score = 141 bits (356), Expect = 3e-39
Identities = 66/76 (86%), Positives = 72/76 (94%)
Frame = +3
Query: 141 SGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQAFNE 320
SGEDATG+VDYAIKSLEDLLC+ +GKPRNIKIGYAQNLAQLE+AFQTNKKK TADQAF
Sbjct: 33 SGEDATGRVDYAIKSLEDLLCVAEGKPRNIKIGYAQNLAQLENAFQTNKKKWTADQAFEN 92
Query: 321 EYFDYLYGIVITGTEW 368
+YFDY+YGIVITGTEW
Sbjct: 93 DYFDYIYGIVITGTEW 108
>gb|EXX57191.1| hypothetical protein RirG_209410 [Rhizophagus irregularis DAOM
197198w]
Length = 191
Score = 141 bits (355), Expect = 6e-39
Identities = 66/79 (83%), Positives = 72/79 (91%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KD+SGEDATG+VDY IKSLEDLLCI +GKPRNIKI YAQNLAQLES FQTNKKK+TADQA
Sbjct: 41 KDVSGEDATGRVDYVIKSLEDLLCIIEGKPRNIKIEYAQNLAQLESTFQTNKKKRTADQA 100
Query: 312 FNEEYFDYLYGIVITGTEW 368
F +YFDY+YGIV TGTEW
Sbjct: 101 FGSDYFDYIYGIVTTGTEW 119
>gb|PKY40192.1| hypothetical protein RhiirA4_453508 [Rhizophagus irregularis]
Length = 381
Score = 142 bits (358), Expect(3) = 6e-39
Identities = 68/79 (86%), Positives = 73/79 (92%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KDISGEDATG+VDY IKSLE+ LCIT+GK RNIKIGYAQNLAQLESAFQTNKKK+TADQA
Sbjct: 168 KDISGEDATGRVDYTIKSLEEFLCITEGKLRNIKIGYAQNLAQLESAFQTNKKKRTADQA 227
Query: 312 FNEEYFDYLYGIVITGTEW 368
FNE+YFDYLY IV TG EW
Sbjct: 228 FNEDYFDYLYSIVTTGAEW 246
Score = 42.4 bits (98), Expect(3) = 6e-39
Identities = 28/54 (51%), Positives = 32/54 (59%), Gaps = 2/54 (3%)
Frame = +2
Query: 359 DGV--VSGSEYQINLTKSTIEDNLELL*SNMXXXXXXXXXXXXDHVTVSVDSSP 514
DG+ SGSEYQINLTKS I++N ELL SN+ D VSV SSP
Sbjct: 254 DGIYCTSGSEYQINLTKSAIKENPELLRSNVKRVIGIIVGLLKDR--VSVGSSP 305
Score = 24.6 bits (52), Expect(3) = 6e-39
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +1
Query: 103 TSQDIFIVLQK 135
TSQDIFIVLQK
Sbjct: 158 TSQDIFIVLQK 168
>gb|POG75728.1| hypothetical protein GLOIN_2v1561751 [Rhizophagus irregularis DAOM
181602=DAOM 197198]
Length = 209
Score = 141 bits (355), Expect = 9e-39
Identities = 66/79 (83%), Positives = 72/79 (91%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KD+SGEDATG+VDY IKSLEDLLCI +GKPRNIKI YAQNLAQLES FQTNKKK+TADQA
Sbjct: 59 KDVSGEDATGRVDYVIKSLEDLLCIIEGKPRNIKIEYAQNLAQLESTFQTNKKKRTADQA 118
Query: 312 FNEEYFDYLYGIVITGTEW 368
F +YFDY+YGIV TGTEW
Sbjct: 119 FGSDYFDYIYGIVTTGTEW 137
>gb|PKY56483.1| hypothetical protein RhiirA4_549253 [Rhizophagus irregularis]
Length = 163
Score = 139 bits (351), Expect = 1e-38
Identities = 65/76 (85%), Positives = 71/76 (93%)
Frame = +3
Query: 141 SGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQAFNE 320
SGEDATG+VDYAIKSLEDLLC+ +GKPRNIKIGYAQNLAQLE+AFQTNKKK TADQAF
Sbjct: 23 SGEDATGRVDYAIKSLEDLLCVAEGKPRNIKIGYAQNLAQLENAFQTNKKKWTADQAFEN 82
Query: 321 EYFDYLYGIVITGTEW 368
+YFDY+YGIV TGTEW
Sbjct: 83 DYFDYIYGIVTTGTEW 98
>dbj|GBC43469.1| f-box/lrr-repeat protein 7-like [Rhizophagus irregularis DAOM
181602]
Length = 886
Score = 143 bits (360), Expect(2) = 6e-38
Identities = 69/75 (92%), Positives = 74/75 (98%)
Frame = +3
Query: 132 KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQNLAQLESAFQTNKKKQTADQA 311
KDISGEDATG+VDYAIKSLE+LLCIT+GKPRNIKIGYAQNLAQLESAFQTNKKK+TADQA
Sbjct: 630 KDISGEDATGRVDYAIKSLEELLCITEGKPRNIKIGYAQNLAQLESAFQTNKKKRTADQA 689
Query: 312 FNEEYFDYLYGIVIT 356
FNE+YFDYLYGIV T
Sbjct: 690 FNEDYFDYLYGIVTT 704
Score = 42.4 bits (98), Expect(2) = 6e-38
Identities = 22/38 (57%), Positives = 23/38 (60%)
Frame = +1
Query: 22 VTDANEATRCEFXXXXXXXXXXXXXXXTSQDIFIVLQK 135
+TDANEATRCEF TSQDIFIVLQK
Sbjct: 593 MTDANEATRCEFISAILHASIAIAKKLTSQDIFIVLQK 630
>gb|POG60228.1| hypothetical protein GLOIN_2v1788170 [Rhizophagus irregularis DAOM
181602=DAOM 197198]
Length = 200
Score = 132 bits (332), Expect = 2e-35
Identities = 69/100 (69%), Positives = 78/100 (78%), Gaps = 7/100 (7%)
Frame = +3
Query: 87 HCQKTHLSRYIHSFT------KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQ 248
HC K + ++ + T K+ISGEDATG+VDY IKSLEDLLCITKGKPRNIKI Y Q
Sbjct: 101 HCMKDIILKFSNVETMTDVNKKNISGEDATGRVDYVIKSLEDLLCITKGKPRNIKIRYVQ 160
Query: 249 NLAQLESAFQTNKKKQTA-DQAFNEEYFDYLYGIVITGTE 365
NLAQL+SAFQTNKKK T DQAF +YFDY+YGIV TGTE
Sbjct: 161 NLAQLKSAFQTNKKKWTVNDQAFGNDYFDYIYGIVTTGTE 200
>gb|PKC53478.1| hypothetical protein RhiirA1_479208 [Rhizophagus irregularis]
gb|PKY34798.1| hypothetical protein RhiirB3_454865 [Rhizophagus irregularis]
Length = 213
Score = 132 bits (333), Expect = 2e-35
Identities = 69/100 (69%), Positives = 78/100 (78%), Gaps = 7/100 (7%)
Frame = +3
Query: 87 HCQKTHLSRYIHSFT------KDISGEDATGQVDYAIKSLEDLLCITKGKPRNIKIGYAQ 248
HC K + ++ + T K+ISGEDATG+VDY IKSLEDLLCITKGKPRNIKI Y Q
Sbjct: 114 HCMKNIILKFSNVKTMTDVNKKNISGEDATGRVDYVIKSLEDLLCITKGKPRNIKIRYVQ 173
Query: 249 NLAQLESAFQTNKKKQTA-DQAFNEEYFDYLYGIVITGTE 365
NLAQL+SAFQTNKKK T DQAF +YFDY+YGIV TGTE
Sbjct: 174 NLAQLKSAFQTNKKKWTVNDQAFGNDYFDYIYGIVTTGTE 213