BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon27_contig00040888
         (1001 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|EXX51096.1| Isy1p [Rhizophagus irregularis DAOM 197198w] >gi|...   495   e-175
gb|PKC11990.1| Rab43 protein [Rhizophagus irregularis] >gi|13081...   476   e-168
ref|XP_016611681.1| hypothetical protein SPPG_01115 [Spizellomyc...   322   e-107
ref|XP_018290964.1| hypothetical protein PHYBLDRAFT_187241 [Phyc...   318   e-105
ref|XP_022796527.1| pre-mRNA-splicing factor ISY1 homolog [Stylo...   315   e-104
gb|ORY04837.1| pre-mRNA-splicing factor ISY1 [Basidiobolus meris...   313   e-103
ref|XP_001637461.1| predicted protein [Nematostella vectensis] >...   313   e-103
gb|ORX58549.1| pre-mRNA-splicing factor ISY1 [Piromyces finnis]       310   e-102
ref|NP_001077032.1| pre-mRNA-splicing factor ISY1 homolog [Danio...   307   e-100
gb|KFH68510.1| hypothetical protein MVEG_05324 [Mortierella vert...   305   e-100
ref|XP_016357851.1| PREDICTED: pre-mRNA-splicing factor ISY1 hom...   305   e-100
gb|ORX79068.1| pre-mRNA-splicing factor ISY1 [Anaeromyces robustus]   303   e-100
ref|XP_021886353.1| Isy1-like splicing factor [Lobosporangium tr...   302   2e-99
ref|XP_019633873.1| PREDICTED: pre-mRNA-splicing factor ISY1 hom...   303   2e-99
ref|XP_018901817.1| PREDICTED: pre-mRNA-splicing factor ISY1 hom...   304   2e-99
gb|ORY59810.1| Rab43 protein [Neocallimastix californiae]             301   5e-99
ref|XP_020894265.1| pre-mRNA-splicing factor ISY1 homolog [Exaip...   302   6e-99
gb|OBZ81401.1| Pre-mRNA-splicing factor ISY1 [Choanephora cucurb...   301   7e-99
ref|XP_017560201.1| PREDICTED: pre-mRNA-splicing factor ISY1 hom...   302   1e-98
ref|XP_013913453.1| PREDICTED: pre-mRNA-splicing factor ISY1 hom...   301   1e-98

>gb|EXX51096.1| Isy1p [Rhizophagus irregularis DAOM 197198w]
 dbj|GBC12889.1| Pre-mRNA-splicing factor ISY1 [Rhizophagus irregularis DAOM 181602]
 gb|PKC67668.1| Rab43 protein [Rhizophagus irregularis]
 gb|PKY43052.1| Rab43 protein [Rhizophagus irregularis]
 gb|POG62067.1| hypothetical protein GLOIN_2v1699223 [Rhizophagus irregularis DAOM
           181602=DAOM 197198]
          Length = 256

 Score =  495 bits (1274), Expect = e-175
 Identities = 247/256 (96%), Positives = 248/256 (96%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV
Sbjct: 1   MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 60

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN
Sbjct: 61  SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 120

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDG+LLEYEREI
Sbjct: 121 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGSLLEYEREI 180

Query: 587 ENKVIAEALEITDETLPXXXXXXXXESTSQLENNSMDIDSSSEKYVSHVAVPSQKEVEEY 766
           ENKVIAEALEITDETLP        ESTSQLENNSMDIDSSSEKYVSHVAVPSQKEVEEY
Sbjct: 181 ENKVIAEALEITDETLPKDKSKKKKESTSQLENNSMDIDSSSEKYVSHVAVPSQKEVEEY 240

Query: 767 LVRRRKRQMLDRYVSE 814
           LVRRRKRQMLDRYVSE
Sbjct: 241 LVRRRKRQMLDRYVSE 256


>gb|PKC11990.1| Rab43 protein [Rhizophagus irregularis]
 gb|PKK76341.1| Rab43 protein [Rhizophagus irregularis]
 gb|PKY18874.1| Rab43 protein [Rhizophagus irregularis]
          Length = 246

 Score =  476 bits (1226), Expect = e-168
 Identities = 237/246 (96%), Positives = 238/246 (96%)
 Frame = +2

Query: 77  MLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKVSKIQDAGLSD 256
           MLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKVSKIQDAGLSD
Sbjct: 1   MLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKVSKIQDAGLSD 60

Query: 257 YQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGNRGYKYFGRAK 436
           YQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGNRGYKYFGRAK
Sbjct: 61  YQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGNRGYKYFGRAK 120

Query: 437 DLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREIENKVIAEALE 616
           DLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDG+LLEYEREIENKVIAEALE
Sbjct: 121 DLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGSLLEYEREIENKVIAEALE 180

Query: 617 ITDETLPXXXXXXXXESTSQLENNSMDIDSSSEKYVSHVAVPSQKEVEEYLVRRRKRQML 796
           ITDETLP        ESTSQLENNSMDIDSSSEKYVSHVAVPSQKEVEEYLVRRRKRQML
Sbjct: 181 ITDETLPKDKSKKKKESTSQLENNSMDIDSSSEKYVSHVAVPSQKEVEEYLVRRRKRQML 240

Query: 797 DRYVSE 814
           DRYVSE
Sbjct: 241 DRYVSE 246


>ref|XP_016611681.1| hypothetical protein SPPG_01115 [Spizellomyces punctatus DAOM
           BR117]
 gb|KND03642.1| hypothetical protein SPPG_01115 [Spizellomyces punctatus DAOM
           BR117]
          Length = 236

 Score =  322 bits (825), Expect = e-107
 Identities = 162/256 (63%), Positives = 194/256 (75%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARNEEKAQSMLYRFREAQA +LG  K  E+RPYLAS+ + LKDAEKWRQQIIREIS+KV
Sbjct: 1   MARNEEKAQSMLYRFREAQAAELGF-KKSEKRPYLASEVHVLKDAEKWRQQIIREISKKV 59

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           SKIQD+GL+DYQ+RDLNDEINKL+REK HW+ +I+ LGGPDYKR+GPK+LD EGKEVPGN
Sbjct: 60  SKIQDSGLTDYQVRDLNDEINKLIREKRHWEYRIRELGGPDYKRMGPKLLDQEGKEVPGN 119

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFGRAKDLPGVR+LFEQE P   K TR DL + VDADYYGYRDEEDG LLEYE EI
Sbjct: 120 RGYKYFGRAKDLPGVRELFEQEVPEAPKPTRQDLSRRVDADYYGYRDEEDGILLEYEAEI 179

Query: 587 ENKVIAEALEITDETLPXXXXXXXXESTSQLENNSMDIDSSSEKYVSHVAVPSQKEVEEY 766
             + + + +                             D+    +V+HV VPSQK+VE +
Sbjct: 180 SRRELGQVM-----------------------------DTPLTDFVAHVPVPSQKDVEAW 210

Query: 767 LVRRRKRQMLDRYVSE 814
           LVRRR++++ DR++S+
Sbjct: 211 LVRRRQQELADRFLSD 226


>ref|XP_018290964.1| hypothetical protein PHYBLDRAFT_187241 [Phycomyces blakesleeanus
           NRRL 1555(-)]
 gb|OAD72924.1| hypothetical protein PHYBLDRAFT_187241 [Phycomyces blakesleeanus
           NRRL 1555(-)]
          Length = 268

 Score =  318 bits (815), Expect = e-105
 Identities = 155/262 (59%), Positives = 195/262 (74%)
 Frame = +2

Query: 50  ARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKVS 229
           ARNEEKAQSMLYRFREAQA +LGL++ KERRP +AS   ++ + E+WR++++ ++SRKVS
Sbjct: 10  ARNEEKAQSMLYRFREAQAAELGLIRKKERRPGMASTVTSVGECERWRREVLGDVSRKVS 69

Query: 230 KIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGNR 409
           KIQD  LSDYQ+RDLNDEINKLMREK HW+ +I+ LGG DY R  PKMLDHEGKEVPGNR
Sbjct: 70  KIQDVSLSDYQVRDLNDEINKLMREKYHWERRIRDLGGADYTRSAPKMLDHEGKEVPGNR 129

Query: 410 GYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREIE 589
           GYKYFGRA+DLPGVR+LFEQEAP P  R+R ++Y+N+DADYYGYRDEEDG L+EYE E E
Sbjct: 130 GYKYFGRARDLPGVRELFEQEAPEPASRSRSEIYRNIDADYYGYRDEEDGALVEYEAEQE 189

Query: 590 NKVIAEALEITDETLPXXXXXXXXESTSQLENNSMDIDSSSEKYVSHVAVPSQKEVEEYL 769
             +   AL   +            E   + E++  +     E+  S  AVP QK++E YL
Sbjct: 190 QDLAERALRAAE------------EQEKKEEDDEKEKAEELEQLRSESAVPDQKDIEAYL 237

Query: 770 VRRRKRQMLDRYVSE*VVQKSQ 835
           V RR++Q+LD+YVSE + Q  Q
Sbjct: 238 VNRRRQQLLDKYVSEDLQQNEQ 259


>ref|XP_022796527.1| pre-mRNA-splicing factor ISY1 homolog [Stylophora pistillata]
          Length = 283

 Score =  315 bits (808), Expect = e-104
 Identities = 156/276 (56%), Positives = 207/276 (75%), Gaps = 13/276 (4%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARN EKA +ML R+ + Q  + G    KERRP+LA++C+NL++AEKWRQQIIRE+++KV
Sbjct: 1   MARNAEKAMTMLARWYDLQRKEAGK-GIKERRPFLATECDNLQEAEKWRQQIIREVAKKV 59

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           ++IQ+AGL +++IRDLNDEINKL+REK HW+D+IK LGGPDY ++GPKMLDHEGKEVPGN
Sbjct: 60  AQIQNAGLGEFRIRDLNDEINKLLREKRHWEDRIKELGGPDYAKIGPKMLDHEGKEVPGN 119

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFG AKDLPGVR+LFEQE P+P ++TR +LYK+VDADYYGYRDE+DG L+  E+E+
Sbjct: 120 RGYKYFGAAKDLPGVRELFEQEPPAPPRKTRGELYKDVDADYYGYRDEDDGVLVPLEQEV 179

Query: 587 ENKVIAEAL----------EITDETLPXXXXXXXXESTSQLENNSMDIDSSSE---KYVS 727
           E + IA AL          E+  E               +  +  M+ D + E    +V+
Sbjct: 180 EKEAIARALDEWERKKEAGELVTEQDQEEENIYAVTKEQEESDEDMETDQTEEGNQPFVA 239

Query: 728 HVAVPSQKEVEEYLVRRRKRQMLDRYVSE*VVQKSQ 835
           HV VPSQKEVEE LV+R+K ++L++Y SE ++Q+SQ
Sbjct: 240 HVPVPSQKEVEEMLVQRKKMELLEKYASETLIQQSQ 275


>gb|ORY04837.1| pre-mRNA-splicing factor ISY1 [Basidiobolus meristosporus CBS
           931.73]
          Length = 284

 Score =  313 bits (803), Expect = e-103
 Identities = 161/283 (56%), Positives = 214/283 (75%), Gaps = 16/283 (5%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARNEEKAQSML+RFR+A+A +LG  K  ERRPYLAS+C +L +AEKWR+QII EISRKV
Sbjct: 1   MARNEEKAQSMLHRFRDARAAELGFNK-NERRPYLASECRSLPEAEKWRRQIINEISRKV 59

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           ++IQ++GLSDYQ+RD+NDEINKL+REK HW+ QIK LGGPDY+R+ P+++D+EGK VPGN
Sbjct: 60  TQIQNSGLSDYQVRDMNDEINKLIREKGHWERQIKDLGGPDYRRIAPRIIDNEGKSVPGN 119

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSP-QKRTRFDLYKNVDADYYGYRDEEDGTLLEYERE 583
           RGYKYFGRA++LPGV++LFE EA    + ++R+++YKNV+ADYYGYRDE+DG LLEYE +
Sbjct: 120 RGYKYFGRARELPGVKELFEVEAAQEVESKSRYEMYKNVNADYYGYRDEDDGILLEYEEK 179

Query: 584 IENKVIAEALEITDETL-------PXXXXXXXXESTSQLENNSM-----DIDSSSEKYVS 727
            E ++ A   E  D  L                E +   E  S+     D+D+S  K+++
Sbjct: 180 QEKELRARLEESNDLILVEYDQGTAKGRKRKGGEGSISREPASISVAEGDVDNSEVKFIA 239

Query: 728 HVAVPSQKEVEEYLVRRRKRQMLDRYVSE*V---VQKSQILTG 847
           HV VPSQK+VE +LVRRRK+ + D+YVSE +   VQ+++ LTG
Sbjct: 240 HVDVPSQKDVEGFLVRRRKQDLFDKYVSEDLEATVQETKELTG 282


>ref|XP_001637461.1| predicted protein [Nematostella vectensis]
 gb|EDO45398.1| predicted protein [Nematostella vectensis]
          Length = 282

 Score =  313 bits (802), Expect = e-103
 Identities = 153/275 (55%), Positives = 207/275 (75%), Gaps = 12/275 (4%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARN EKA +ML R+ + Q  + G    KERRP+LA++C+NL++AEKWR QIIRE+++KV
Sbjct: 1   MARNAEKAMTMLSRWYDVQRKEAGK-GIKERRPFLATECDNLQEAEKWRHQIIREVAKKV 59

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           ++IQ+AGL +++IRDLNDEINKL+REK HW+D+IK LGGPDY ++GPKMLDHEGKEVPGN
Sbjct: 60  AQIQNAGLGEFRIRDLNDEINKLLREKRHWEDRIKELGGPDYGKIGPKMLDHEGKEVPGN 119

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFG AK+LPGVR+LFEQE P+P ++TR +LYK++DADYYGYRDE+DG L+  E+E 
Sbjct: 120 RGYKYFGAAKELPGVRELFEQEPPAPPRKTRGELYKDIDADYYGYRDEDDGVLVPIEQEA 179

Query: 587 ENKVIAEALEITDETLPXXXXXXXXESTSQL---------ENNSMDIDSSSEK---YVSH 730
           E + I+ A++               E    +         ++  MD +  +EK   +V+H
Sbjct: 180 EEEAISRAMDEWKRKKEEGQVELEDEDEEDIYAVKPGPEGDDEDMDTEEGAEKEQSFVAH 239

Query: 731 VAVPSQKEVEEYLVRRRKRQMLDRYVSE*VVQKSQ 835
           V VPSQKEVEE LV+R+KR++LD+Y SE ++Q+SQ
Sbjct: 240 VPVPSQKEVEELLVQRKKRELLDKYASEALMQQSQ 274


>gb|ORX58549.1| pre-mRNA-splicing factor ISY1 [Piromyces finnis]
          Length = 246

 Score =  310 bits (794), Expect = e-102
 Identities = 150/264 (56%), Positives = 200/264 (75%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARNEEKAQSML+RFREAQA++LGL+K +E+RPYLA+ C+N+KD E WR+QII+EIS+KV
Sbjct: 1   MARNEEKAQSMLHRFREAQAIELGLLKKEEKRPYLATLCDNVKDCEYWRKQIIKEISKKV 60

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           SKIQD    DYQIRDLNDEINKL+REK HW+++I  LGGP+YK++G K LD EGKEVPG+
Sbjct: 61  SKIQD----DYQIRDLNDEINKLIREKYHWENRILELGGPNYKKIGQKTLDKEGKEVPGS 116

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFGRA+DLPGVR+LFE E    + +TR++LY+N+DADYYGYRDEED  LLEYE E+
Sbjct: 117 RGYKYFGRARDLPGVRELFEPEVSEIRSKTRYELYQNIDADYYGYRDEEDDILLEYETEL 176

Query: 587 ENKVIAEALEITDETLPXXXXXXXXESTSQLENNSMDIDSSSEKYVSHVAVPSQKEVEEY 766
             + + E L+                    +ENN+ D++ +   +V H+ +PSQ EVE+Y
Sbjct: 177 RKQYVDEILK-----------------EEGIENNNKDVNYNEYDFVEHLYIPSQSEVEKY 219

Query: 767 LVRRRKRQMLDRYVSE*VVQKSQI 838
           L+ ++K ++  +Y+SE    K++I
Sbjct: 220 LIEQKKEELTKKYLSEAASLKAEI 243


>ref|NP_001077032.1| pre-mRNA-splicing factor ISY1 homolog [Danio rerio]
 gb|AAI15266.1| Rab43 protein [Danio rerio]
          Length = 285

 Score =  307 bits (786), Expect = e-100
 Identities = 156/280 (55%), Positives = 200/280 (71%), Gaps = 17/280 (6%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARN EKA + L RFR+AQ  +    K KERRPYLAS+CN L  AEKWR+QII EIS+KV
Sbjct: 1   MARNAEKAMTALARFRQAQLEE---GKVKERRPYLASECNELPKAEKWRRQIISEISKKV 57

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           ++IQ+AGL +++IRDLNDEINKL+REK HW+ +IK LGGPDY+R GPKMLDHEGKEVPGN
Sbjct: 58  AQIQNAGLGEFKIRDLNDEINKLLREKGHWEVRIKELGGPDYRRFGPKMLDHEGKEVPGN 117

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFG AKDLPGVR+LFE E   P ++TR +L K++DADYYGYRDE+DG LL  E+E 
Sbjct: 118 RGYKYFGAAKDLPGVRELFESEPVPPPRKTRAELMKDIDADYYGYRDEDDGVLLPLEQEY 177

Query: 587 ENKVIAEALE-----------------ITDETLPXXXXXXXXESTSQLENNSMDIDSSSE 715
           E +V+AEA+E                  T+E           E +     + M+ +  ++
Sbjct: 178 EKQVMAEAVEKWKADKEARLAVGGAVKDTEEEEEYIYAVRDEERSDDETGDQMEGEDGAQ 237

Query: 716 KYVSHVAVPSQKEVEEYLVRRRKRQMLDRYVSE*VVQKSQ 835
            ++SHV VPSQKE+EE LVRR+K ++L +Y SE ++ +SQ
Sbjct: 238 SFISHVPVPSQKEIEEALVRRKKMELLQKYASESLMAQSQ 277


>gb|KFH68510.1| hypothetical protein MVEG_05324 [Mortierella verticillata NRRL
           6337]
          Length = 246

 Score =  305 bits (781), Expect = e-100
 Identities = 157/258 (60%), Positives = 193/258 (74%), Gaps = 2/258 (0%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARNEEKAQSMLYRFREA+  +LG  + KERRPY  S+  +L +AEKWR+ +I +IS+KV
Sbjct: 1   MARNEEKAQSMLYRFREAKNAELGGSRYKERRPYRVSEVTSLVEAEKWRRNVIGDISKKV 60

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           SKIQDAGLSD+QIRDLNDEINKLMREK  W+  IK+LGG DY+  GPK LD +GKEVPG 
Sbjct: 61  SKIQDAGLSDFQIRDLNDEINKLMREKYRWEQHIKQLGGVDYRVTGPKTLDAQGKEVPGA 120

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFGRAKDLPGV++LF+  AP+ ++++R +L +NVDADYYGYRDEEDGTLLEYE+E 
Sbjct: 121 RGYKYFGRAKDLPGVKELFQPRAPTLKEKSRAELLRNVDADYYGYRDEEDGTLLEYEQEQ 180

Query: 587 ENKVIAEALEITDETLPXXXXXXXXESTSQLENNSMDIDSSSEKY--VSHVAVPSQKEVE 760
           E   + +ALE  +E                    +MD+D    +   V  + VPSQKEVE
Sbjct: 181 EELAMEKALEEAEEQ----------------NEGAMDVDDKEAQTSPVGPLFVPSQKEVE 224

Query: 761 EYLVRRRKRQMLDRYVSE 814
           EYLV+RRK+ +LDRYVSE
Sbjct: 225 EYLVKRRKQAILDRYVSE 242


>ref|XP_016357851.1| PREDICTED: pre-mRNA-splicing factor ISY1 homolog [Sinocyclocheilus
           anshuiensis]
          Length = 284

 Score =  305 bits (782), Expect = e-100
 Identities = 156/280 (55%), Positives = 206/280 (73%), Gaps = 17/280 (6%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARN EKA + L RFR+AQ ++ G +K  ERRP+LAS+CN L  AEKWR+QII EIS+KV
Sbjct: 1   MARNAEKAMTALARFRQAQ-LEEGRIK--ERRPFLASECNELPKAEKWRRQIISEISKKV 57

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           ++IQ+AGL +++IRDLNDEINKL+REK HW+ +IK LGGPDY+RVGPKMLDHEGKEVPGN
Sbjct: 58  AQIQNAGLGEFKIRDLNDEINKLLREKGHWEVRIKELGGPDYRRVGPKMLDHEGKEVPGN 117

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFG AKDLPGVR+LFE+E  +P ++TR +L K++DADYYGYRDE+DG L+  E+E 
Sbjct: 118 RGYKYFGAAKDLPGVRELFEKEPVAPPRKTRAELMKDIDADYYGYRDEDDGALVPLEQEY 177

Query: 587 ENKVIAEALE-----------------ITDETLPXXXXXXXXESTSQLENNSMDIDSSSE 715
           E +VIAEA+E                  T+E           ES  ++    M+ +  S+
Sbjct: 178 EKQVIAEAVEKWKADKEARSAVGGGEKDTEEEEEYIYAVRDEESDDEM-GEQMEGEDGSQ 236

Query: 716 KYVSHVAVPSQKEVEEYLVRRRKRQMLDRYVSE*VVQKSQ 835
            +++HV VPSQKE+EE LVRR+K ++L +Y SE ++ +S+
Sbjct: 237 SFIAHVPVPSQKEIEEALVRRKKMELLQKYASESLMAQSE 276


>gb|ORX79068.1| pre-mRNA-splicing factor ISY1 [Anaeromyces robustus]
          Length = 254

 Score =  303 bits (777), Expect = e-100
 Identities = 144/256 (56%), Positives = 196/256 (76%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARNEEKAQSML+RFREAQA++LGL+K +E+RPYLA+ C+N+KD E WR+QII+EIS+KV
Sbjct: 1   MARNEEKAQSMLHRFREAQAIELGLLKKEEKRPYLATLCDNVKDCEYWRKQIIKEISKKV 60

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           SKIQD  L+DYQIRDLNDEINKL+REK HW+++I  LGGP+Y+++G K LD EG+E PG+
Sbjct: 61  SKIQDVQLTDYQIRDLNDEINKLIREKYHWENRILELGGPNYRKIGEKTLDREGREAPGS 120

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFGRA+DLPGVR+LFE E      +TR++LY+N+DADYYGYRDEED  LLEYE E+
Sbjct: 121 RGYKYFGRARDLPGVRELFEPEVSETPAKTRYELYQNIDADYYGYRDEEDDVLLEYETEL 180

Query: 587 ENKVIAEALEITDETLPXXXXXXXXESTSQLENNSMDIDSSSEKYVSHVAVPSQKEVEEY 766
               + E L+  +E +               +NN+ +++ S   +V  + +P+Q EVE+Y
Sbjct: 181 RKHYVDEILK--EEKIDMDD-----------DNNNEEVNYSEYDFVEPLYIPTQSEVEKY 227

Query: 767 LVRRRKRQMLDRYVSE 814
           L+ ++K ++  +Y+SE
Sbjct: 228 LIEQKKEELTKKYLSE 243


>ref|XP_021886353.1| Isy1-like splicing factor [Lobosporangium transversale]
 gb|ORZ28680.1| Isy1-like splicing factor [Lobosporangium transversale]
          Length = 248

 Score =  302 bits (774), Expect = 2e-99
 Identities = 155/264 (58%), Positives = 198/264 (75%), Gaps = 2/264 (0%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARNEEKAQSMLYRFREA+  +LG  K  ERRP+  ++ ++L +AEKWR+ +I +ISRKV
Sbjct: 1   MARNEEKAQSMLYRFREAKNAELGGSKKPERRPFRITEVSSLTEAEKWRRNVIGDISRKV 60

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           SKIQD GLSD+QIRDLNDEINKLMREK  W+ QIK LGG DY+  GPK LD +GKEVPG 
Sbjct: 61  SKIQDPGLSDFQIRDLNDEINKLMREKYRWEQQIKDLGGVDYRITGPKTLDAQGKEVPGA 120

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFGRAKDLPGV++LF+ +  + +++TR +LY+++DADYYGYRDEEDGTLLE+E E 
Sbjct: 121 RGYKYFGRAKDLPGVKELFQPKPLTLKEKTRAELYRHIDADYYGYRDEEDGTLLEFEEEQ 180

Query: 587 ENKVIAEALEITDETLPXXXXXXXXESTSQLENNSMDIDSSSE--KYVSHVAVPSQKEVE 760
           E   + +ALE  DE                   + MD+DS +E  K +  + VPSQKEVE
Sbjct: 181 EEIAMKKALEDADED----------------AGSKMDVDSQTERTKPLGRLYVPSQKEVE 224

Query: 761 EYLVRRRKRQMLDRYVSE*VVQKS 832
           EYLV+RRK+ ++DR+V++ VVQ +
Sbjct: 225 EYLVKRRKQAIMDRFVNDDVVQSA 248


>ref|XP_019633873.1| PREDICTED: pre-mRNA-splicing factor ISY1 homolog [Branchiostoma
           belcheri]
          Length = 279

 Score =  303 bits (777), Expect = 2e-99
 Identities = 153/274 (55%), Positives = 203/274 (74%), Gaps = 11/274 (4%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARN EKA + L R+R  Q  Q    K KERRP+LAS+ N+L  AEKWR QIIRE+++KV
Sbjct: 1   MARNAEKAMTTLARWRNMQMDQ---GKVKERRPFLASEENDLGKAEKWRHQIIREVAKKV 57

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           S+IQ+AGL +++IRDLNDEINKL+REK HW+++IK LGGPDY+RVGPKMLD EGKEVPGN
Sbjct: 58  SQIQNAGLGEFRIRDLNDEINKLLREKKHWEERIKELGGPDYERVGPKMLDQEGKEVPGN 117

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFG AKDLPGVR+LFEQE P+P +RTR +LYK VDADYYGYRDE+DG L+  E+++
Sbjct: 118 RGYKYFGAAKDLPGVRELFEQEPPAPPRRTRAELYKEVDADYYGYRDEDDGVLVPLEQDL 177

Query: 587 ENKVIAEALE----------ITDETLPXXXXXXXXESTSQLENNS-MDIDSSSEKYVSHV 733
           E +V+AEA+           + D            ES  +    S M+ +   +++++HV
Sbjct: 178 EKEVVAEAVAEWKSQKEAGLLKDVEEEEDIYAVEEESDEEETGESKMETEEGQQQFIAHV 237

Query: 734 AVPSQKEVEEYLVRRRKRQMLDRYVSE*VVQKSQ 835
            VPSQKEVEE L+RR+K ++L +Y S+ ++++S+
Sbjct: 238 PVPSQKEVEEALLRRKKMELLQKYASDSLLEQSK 271


>ref|XP_018901817.1| PREDICTED: pre-mRNA-splicing factor ISY1 homolog [Bemisia tabaci]
          Length = 293

 Score =  304 bits (778), Expect = 2e-99
 Identities = 156/280 (55%), Positives = 201/280 (71%), Gaps = 17/280 (6%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARN EKA + L R+R AQ  + G  + KERRPYLASDC  L  AEKWR QIIRE++RKV
Sbjct: 1   MARNAEKAMTTLARWRAAQVHEKG-TEEKERRPYLASDCKELPKAEKWRMQIIREVARKV 59

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           ++IQ+AGL +++IRDLNDEINKL+REKSHW+ QIK LGGPDY RVGP+MLDHEGKEVPGN
Sbjct: 60  AQIQNAGLGEFRIRDLNDEINKLIREKSHWEAQIKELGGPDYARVGPRMLDHEGKEVPGN 119

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFG AKDLPGVR+LFEQE P P ++TR +L K++DADYYGYRD++DG LL  E   
Sbjct: 120 RGYKYFGAAKDLPGVRELFEQEPPPPPRKTRGELMKDIDADYYGYRDDDDGLLLPAEMTE 179

Query: 587 ENKVIA----------EALEITDE--TLPXXXXXXXXESTSQLENN-----SMDIDSSSE 715
           E K IA          E++E+ +E  T          ES    EN       +  + S  
Sbjct: 180 EKKAIAKAVADWKNRVESMEVGEESDTEEVEKSRLQAESGDSSENEGEEDAGLQNELSGS 239

Query: 716 KYVSHVAVPSQKEVEEYLVRRRKRQMLDRYVSE*VVQKSQ 835
           K+++HV VPSQK++E+ L+ R+K+++L+RY SE +V++ +
Sbjct: 240 KFIAHVPVPSQKQIEQALIERKKQELLNRYASEALVKEEE 279


>gb|ORY59810.1| Rab43 protein [Neocallimastix californiae]
          Length = 252

 Score =  301 bits (772), Expect = 5e-99
 Identities = 147/264 (55%), Positives = 197/264 (74%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARNEEKAQSML+RFREAQA++LGL+K +E+RPYLA+ C+N+KD E WR+QII+EIS KV
Sbjct: 1   MARNEEKAQSMLHRFREAQAIELGLLKKEEKRPYLATLCDNVKDCEYWRKQIIKEISAKV 60

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           SKIQD  L+DYQIRDLNDEINKL+REK HW+++I  LGGP+YK++G K LD EGKE PG+
Sbjct: 61  SKIQDVQLTDYQIRDLNDEINKLIREKYHWENRILELGGPNYKKIGQKSLDKEGKEAPGS 120

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFGRA+DLPGVR+LFE E      +TR++LY+N+DADYYGYRDEED  LLEYE E+
Sbjct: 121 RGYKYFGRARDLPGVRELFEPEVGEVPTKTRYELYQNIDADYYGYRDEEDDVLLEYETEL 180

Query: 587 ENKVIAEALEITDETLPXXXXXXXXESTSQLENNSMDIDSSSEKYVSHVAVPSQKEVEEY 766
             + + E L+               E   + + N  +++ S   +V  + +P+Q EVE+Y
Sbjct: 181 RRQYVDEILK--------------AEKIGEDDENK-EVNYSEYDFVEPLYIPNQSEVEKY 225

Query: 767 LVRRRKRQMLDRYVSE*VVQKSQI 838
           L+ ++K ++  +Y+SE    +S+I
Sbjct: 226 LIEQKKEELTKKYLSESASLQSEI 249


>ref|XP_020894265.1| pre-mRNA-splicing factor ISY1 homolog [Exaiptasia pallida]
          Length = 281

 Score =  302 bits (774), Expect = 6e-99
 Identities = 150/274 (54%), Positives = 202/274 (73%), Gaps = 11/274 (4%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARN EKA +ML R+ + +  + G   P ERRP+LA++C+NL++AEKWR QIIRE+++KV
Sbjct: 1   MARNAEKAMTMLSRWYDVKRKEAGKGIP-ERRPFLATECDNLQEAEKWRHQIIREVAKKV 59

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           ++IQ+AGL +++IRDLNDEINKL+REK HW+D+I  LGGP+YK++GPKMLDHEGKEVPGN
Sbjct: 60  AQIQNAGLGEFRIRDLNDEINKLLREKRHWEDRIVELGGPNYKKIGPKMLDHEGKEVPGN 119

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFG AK+LPGVR+LFEQE P   ++TR +LYK+VDADYYGYRDE+DG L+  E+E+
Sbjct: 120 RGYKYFGAAKELPGVRELFEQEPPKAPRKTRGELYKDVDADYYGYRDEDDGVLVPIEQEV 179

Query: 587 ENKVIAEA-----------LEITDETLPXXXXXXXXESTSQLENNSMDIDSSSEKYVSHV 733
           E   IA A           +E  DE           +  S  E  + +    S+ +V HV
Sbjct: 180 EKDAIARAIEEYQAKKEQEMEEDDEEEEDIYAVQPEQEGSDEEMATDEGGEGSQSFVXHV 239

Query: 734 AVPSQKEVEEYLVRRRKRQMLDRYVSE*VVQKSQ 835
            VPSQ+EVE+ LV+R+K ++LD+Y SE ++Q+SQ
Sbjct: 240 PVPSQQEVEQMLVQRKKMELLDKYASEALMQQSQ 273


>gb|OBZ81401.1| Pre-mRNA-splicing factor ISY1 [Choanephora cucurbitarum]
          Length = 266

 Score =  301 bits (772), Expect = 7e-99
 Identities = 148/264 (56%), Positives = 195/264 (73%), Gaps = 1/264 (0%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARNEEKAQSMLYRFREAQA +LGL KP+ RRP  A   + + +AEKWR+ ++ E+SRK+
Sbjct: 1   MARNEEKAQSMLYRFREAQAAELGLGKPRLRRPAFAGVVDTIAEAEKWRRDLLSEVSRKI 60

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           SKIQD  LSDYQ+RDLND+INKLM +K HW+ +IK LGGPDY R GP+M  +EG+EVPG 
Sbjct: 61  SKIQDVSLSDYQVRDLNDDINKLMGQKYHWERRIKELGGPDYTRSGPRMFSYEGREVPGT 120

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGY+Y+GRA+DLPGVR+LFE+ A   Q RTR ++ +++DADYYGYRDEEDG LL YE  +
Sbjct: 121 RGYRYYGRARDLPGVRELFEKAASEAQPRTRTEINRHIDADYYGYRDEEDGQLLAYENAL 180

Query: 587 ENKVIAEALEITDETLPXXXXXXXXESTSQLENNSMDIDSSS-EKYVSHVAVPSQKEVEE 763
           EN++ A+ L   D            E+ ++  + +M+I+    E  V+   VPSQKE+E 
Sbjct: 181 ENELQAKLLSAPD-------IKEEEEAQNEASSTAMEIEQKEVETNVADSHVPSQKEIEA 233

Query: 764 YLVRRRKRQMLDRYVSE*VVQKSQ 835
           YLV+RRK+Q+LD+YVSE + Q  +
Sbjct: 234 YLVQRRKQQLLDKYVSEDMKQNEE 257


>ref|XP_017560201.1| PREDICTED: pre-mRNA-splicing factor ISY1 homolog [Pygocentrus
           nattereri]
          Length = 285

 Score =  302 bits (773), Expect = 1e-98
 Identities = 153/280 (54%), Positives = 199/280 (71%), Gaps = 17/280 (6%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARN EKA + L RFR+AQ  +    K KERRP+LAS+CN L  AEKWR+QII EIS+KV
Sbjct: 1   MARNAEKAMTALARFRQAQLEE---GKVKERRPFLASECNELPKAEKWRRQIISEISKKV 57

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           ++IQ+AGL +++IRDLNDEINKL+REK HW+ +IK LGGPDY RVGPKMLDHEGKEVPGN
Sbjct: 58  AQIQNAGLGEFKIRDLNDEINKLLREKGHWEVRIKELGGPDYARVGPKMLDHEGKEVPGN 117

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFG AKDLPGVR+LFE+E   P ++TR +L K++DADYYGYRDE+DG L+  E+E 
Sbjct: 118 RGYKYFGAAKDLPGVRELFEKEPVPPPRKTRAELMKDIDADYYGYRDEDDGVLVPLEQEY 177

Query: 587 ENKVIAEALE-----------------ITDETLPXXXXXXXXESTSQLENNSMDIDSSSE 715
           E +VIAEA+E                   +E           E +       M+ +  ++
Sbjct: 178 EKQVIAEAIEKWKEEKEARLAGGGGAKEVEEEEEYIYAIKDDEQSDDEMREQMEGEDGAQ 237

Query: 716 KYVSHVAVPSQKEVEEYLVRRRKRQMLDRYVSE*VVQKSQ 835
            +++HV VPSQKE+EE LVRR+K ++L +Y SE ++ +S+
Sbjct: 238 TFIAHVPVPSQKEIEEALVRRKKMELLQKYASESLMAQSE 277


>ref|XP_013913453.1| PREDICTED: pre-mRNA-splicing factor ISY1 homolog [Thamnophis
           sirtalis]
          Length = 282

 Score =  301 bits (772), Expect = 1e-98
 Identities = 157/277 (56%), Positives = 199/277 (71%), Gaps = 14/277 (5%)
 Frame = +2

Query: 47  MARNEEKAQSMLYRFREAQAVQLGLVKPKERRPYLASDCNNLKDAEKWRQQIIREISRKV 226
           MARN EKA + L RFR+AQ  +    K KERRP+LAS+CN L  AEKWR+QII EIS+KV
Sbjct: 1   MARNAEKAMTALARFRQAQLEE---GKLKERRPFLASECNELPKAEKWRRQIIGEISKKV 57

Query: 227 SKIQDAGLSDYQIRDLNDEINKLMREKSHWDDQIKRLGGPDYKRVGPKMLDHEGKEVPGN 406
           ++IQ+AGL +++IRDLNDEINKL+REK HW+ +IK LGGPDY R+GPKMLDHEGKEVPGN
Sbjct: 58  AQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYARIGPKMLDHEGKEVPGN 117

Query: 407 RGYKYFGRAKDLPGVRDLFEQEAPSPQKRTRFDLYKNVDADYYGYRDEEDGTLLEYEREI 586
           RGYKYFG AKDLPGVR+LFE+E   P ++TR +L K +DA+YYGYRDE+DG L   E+E 
Sbjct: 118 RGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDAEYYGYRDEDDGVLEPLEQEH 177

Query: 587 ENKVIAEALEITDETLPXXXXXXXXESTSQLEN------------NSMDIDS--SSEKYV 724
           E KVIAEA+E               E   + EN            N M  DS  S +K++
Sbjct: 178 EKKVIAEAVERWKVEREAHLARGDKEEEEEEENIYAVNDNESDEENGMKTDSEDSQQKFI 237

Query: 725 SHVAVPSQKEVEEYLVRRRKRQMLDRYVSE*VVQKSQ 835
           +HV VPSQ+E+EE LVRR+K ++L +Y SE ++ +S+
Sbjct: 238 AHVPVPSQQEIEEALVRRKKMELLQKYASEALLAQSE 274