BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon27_contig00018729
         (1930 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020247136.1| LOW QUALITY PROTEIN: pullulanase 1, chloropl...   959   0.0  
gb|ONK57269.1| uncharacterized protein A4U43_C10F18340 [Asparagu...   939   0.0  
ref|XP_010918919.1| PREDICTED: pullulanase 1, chloroplastic isof...   901   0.0  
ref|XP_010267261.1| PREDICTED: pullulanase 1, chloroplastic isof...   900   0.0  
ref|XP_010267260.1| PREDICTED: pullulanase 1, chloroplastic isof...   900   0.0  
ref|XP_010267258.1| PREDICTED: pullulanase 1, chloroplastic isof...   900   0.0  
gb|OVA17964.1| Glycoside hydrolase [Macleaya cordata]                 889   0.0  
ref|XP_020084106.1| pullulanase 1, chloroplastic [Ananas comosus]     885   0.0  
ref|XP_021295296.1| pullulanase 1, chloroplastic isoform X3 [Her...   875   0.0  
ref|XP_020703866.1| pullulanase 1, chloroplastic [Dendrobium cat...   877   0.0  
ref|XP_021295295.1| pullulanase 1, chloroplastic isoform X2 [Her...   875   0.0  
ref|XP_021295293.1| pullulanase 1, chloroplastic isoform X1 [Her...   875   0.0  
ref|XP_017984815.1| PREDICTED: pullulanase 1, chloroplastic isof...   874   0.0  
ref|XP_007010038.2| PREDICTED: pullulanase 1, chloroplastic isof...   874   0.0  
gb|EOY18848.1| Limit dextrinase [Theobroma cacao]                     874   0.0  
ref|XP_019079995.1| PREDICTED: pullulanase 1, chloroplastic isof...   868   0.0  
ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isof...   868   0.0  
ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isof...   868   0.0  
dbj|GAV67655.1| Alpha-amylase domain-containing protein/CBM_48 d...   863   0.0  
ref|XP_007220271.1| pullulanase 1, chloroplastic [Prunus persica...   867   0.0  

>ref|XP_020247136.1| LOW QUALITY PROTEIN: pullulanase 1, chloroplastic [Asparagus
            officinalis]
          Length = 960

 Score =  959 bits (2480), Expect = 0.0
 Identities = 464/544 (85%), Positives = 498/544 (91%)
 Frame = +2

Query: 299  VVHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKV 478
            VV AHVYK P G D+LD +QL+E++GVWSA GPRSWEGCYYVYEVSVYHPSTS+IETCKV
Sbjct: 223  VVCAHVYKGPLGEDQLDPMQLEEVNGVWSAAGPRSWEGCYYVYEVSVYHPSTSRIETCKV 282

Query: 479  NDPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASD 658
            NDPYARG+SSDGMRTLLVNLDC + KP GWD+LA  KP+  SF+DISIYELHVRDFSASD
Sbjct: 283  NDPYARGLSSDGMRTLLVNLDCGKFKPPGWDDLANGKPEFHSFADISIYELHVRDFSASD 342

Query: 659  ETVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQ 838
            ETV+P+FRGGFLAFTS+DSAGV HLKKLSDAGLTHVHLLPCFQFGGVDDDR KW+QLD+ 
Sbjct: 343  ETVDPNFRGGFLAFTSQDSAGVSHLKKLSDAGLTHVHLLPCFQFGGVDDDRIKWEQLDYD 402

Query: 839  ELAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQA 1018
            EL+K P DSDKQQ  +TSI DQDGYNWGYNPVL+GVPKGSYASNPNG+SRIIDFRKMIQA
Sbjct: 403  ELSKQPPDSDKQQGEVTSIQDQDGYNWGYNPVLFGVPKGSYASNPNGESRIIDFRKMIQA 462

Query: 1019 LNGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHL 1198
            LN IGLRVVLDVVYNH++ASGPLD  +V   VVPGYY+RRN+DG+IENS CINNTASEH 
Sbjct: 463  LNRIGLRVVLDVVYNHLYASGPLDLSAVKLXVVPGYYLRRNVDGYIENSACINNTASEHF 522

Query: 1199 MVERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIY 1378
            MVERLIIDDLLCWAV YKVDGFRFDLMGHIMKSTM+KAKSALQSLSKDENGVDGSKIYIY
Sbjct: 523  MVERLIIDDLLCWAVAYKVDGFRFDLMGHIMKSTMVKAKSALQSLSKDENGVDGSKIYIY 582

Query: 1379 GEGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQ 1558
            GEGWDFGEVA N RGINASQFNI GTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQ
Sbjct: 583  GEGWDFGEVANNGRGINASQFNIGGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQ 642

Query: 1559 PNAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYA 1738
            PN YD+GSK VTD+MLS SVDHIQ+GLAANL DFVLT H G A+KGSEI THDG+ VGYA
Sbjct: 643  PNGYDHGSKRVTDNMLSTSVDHIQIGLAANLRDFVLTSHTGAAMKGSEILTHDGVAVGYA 702

Query: 1739 LCPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDE 1918
            LCP ET+NYVSAHDNETLFD I LKTAEEI VDDRCR+NHLATSIVALSQGVPFFHSGDE
Sbjct: 703  LCPTETINYVSAHDNETLFDIISLKTAEEILVDDRCRINHLATSIVALSQGVPFFHSGDE 762

Query: 1919 ILRS 1930
            ILRS
Sbjct: 763  ILRS 766



 Score =  170 bits (431), Expect = 1e-40
 Identities = 84/99 (84%), Positives = 85/99 (85%)
 Frame = +1

Query: 7   KFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNATG 186
           K  LE  PCGLPENV+QKFPHIK YKAFHVP SVDVG LVKCQLAVVSF   GKC NATG
Sbjct: 127 KVLLEQEPCGLPENVIQKFPHIKHYKAFHVPLSVDVGSLVKCQLAVVSFCARGKCKNATG 186

Query: 187 LQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGC 303
           LQLPGVLDDMFAY GPLGAVFSEEAITL LWAPTAQV C
Sbjct: 187 LQLPGVLDDMFAYKGPLGAVFSEEAITLNLWAPTAQVVC 225


>gb|ONK57269.1| uncharacterized protein A4U43_C10F18340 [Asparagus officinalis]
          Length = 947

 Score =  939 bits (2428), Expect = 0.0
 Identities = 457/544 (84%), Positives = 490/544 (90%)
 Frame = +2

Query: 299  VVHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKV 478
            VV AHVYK P G D+LD +QL+E++GVWSA GPRSWEGCYYVYEVSVYHPSTS+IETCKV
Sbjct: 223  VVCAHVYKGPLGEDQLDPMQLEEVNGVWSAAGPRSWEGCYYVYEVSVYHPSTSRIETCKV 282

Query: 479  NDPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASD 658
            NDPYARG+SSDGMRTLLVNLDC + KP GWD+LA  KP+  SF+DISIYELHVRDFSASD
Sbjct: 283  NDPYARGLSSDGMRTLLVNLDCGKFKPPGWDDLANGKPEFHSFADISIYELHVRDFSASD 342

Query: 659  ETVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQ 838
            ETV+P+FRGGFLAFTS+DSAGV HLKKLSDAGLTHVHLLPCFQFGGVDDDR KW+QLD+ 
Sbjct: 343  ETVDPNFRGGFLAFTSQDSAGVSHLKKLSDAGLTHVHLLPCFQFGGVDDDRIKWEQLDYD 402

Query: 839  ELAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQA 1018
            EL+K P DSDKQQ  +TSI DQDGYNWGYNPVL+GVPKGSYASNPNG+SRIIDFRKMIQA
Sbjct: 403  ELSKQPPDSDKQQGEVTSIQDQDGYNWGYNPVLFGVPKGSYASNPNGESRIIDFRKMIQA 462

Query: 1019 LNGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHL 1198
            LN IGLRVVLDVVYNH++             VVPGYY+RRN+DG+IENS CINNTASEH 
Sbjct: 463  LNRIGLRVVLDVVYNHLY-------------VVPGYYLRRNVDGYIENSACINNTASEHF 509

Query: 1199 MVERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIY 1378
            MVERLIIDDLLCWAV YKVDGFRFDLMGHIMKSTM+KAKSALQSLSKDENGVDGSKIYIY
Sbjct: 510  MVERLIIDDLLCWAVAYKVDGFRFDLMGHIMKSTMVKAKSALQSLSKDENGVDGSKIYIY 569

Query: 1379 GEGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQ 1558
            GEGWDFGEVA N RGINASQFNI GTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQ
Sbjct: 570  GEGWDFGEVANNGRGINASQFNIGGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQ 629

Query: 1559 PNAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYA 1738
            PN YD+GSK VTD+MLS SVDHIQ+GLAANL DFVLT H G A+KGSEI THDG+ VGYA
Sbjct: 630  PNGYDHGSKRVTDNMLSTSVDHIQIGLAANLRDFVLTSHTGAAMKGSEILTHDGVAVGYA 689

Query: 1739 LCPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDE 1918
            LCP ET+NYVSAHDNETLFD I LKTAEEI VDDRCR+NHLATSIVALSQGVPFFHSGDE
Sbjct: 690  LCPTETINYVSAHDNETLFDIISLKTAEEILVDDRCRINHLATSIVALSQGVPFFHSGDE 749

Query: 1919 ILRS 1930
            ILRS
Sbjct: 750  ILRS 753



 Score =  170 bits (431), Expect = 1e-40
 Identities = 84/99 (84%), Positives = 85/99 (85%)
 Frame = +1

Query: 7   KFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNATG 186
           K  LE  PCGLPENV+QKFPHIK YKAFHVP SVDVG LVKCQLAVVSF   GKC NATG
Sbjct: 127 KVLLEQEPCGLPENVIQKFPHIKHYKAFHVPLSVDVGSLVKCQLAVVSFCARGKCKNATG 186

Query: 187 LQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGC 303
           LQLPGVLDDMFAY GPLGAVFSEEAITL LWAPTAQV C
Sbjct: 187 LQLPGVLDDMFAYKGPLGAVFSEEAITLNLWAPTAQVVC 225


>ref|XP_010918919.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Elaeis
            guineensis]
          Length = 965

 Score =  901 bits (2328), Expect = 0.0
 Identities = 427/539 (79%), Positives = 482/539 (89%)
 Frame = +2

Query: 314  VYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVNDPYA 493
            +YK+PSGG+ L+SVQL+E  GVWSAVGP+SWEGCYYVYEVSVYHPSTSQIE C V DPYA
Sbjct: 233  IYKEPSGGEPLESVQLEEFGGVWSAVGPKSWEGCYYVYEVSVYHPSTSQIEKCTVTDPYA 292

Query: 494  RGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDETVNP 673
            RG+SS+G RT L+NLD E LKP GWD+LA+EKPDL SFSDISIYELH+RDFSASD TVN 
Sbjct: 293  RGLSSNGKRTWLINLDSENLKPEGWDKLADEKPDLLSFSDISIYELHIRDFSASDHTVNC 352

Query: 674  DFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQELAKL 853
            D +GG+LAFTS++SAG+ HLKKLS+AGLTHVHLLP F F GVDDD+  WK LD  E+AKL
Sbjct: 353  DIQGGYLAFTSQESAGICHLKKLSNAGLTHVHLLPSFHFAGVDDDKSNWKYLDDGEMAKL 412

Query: 854  PSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQALNGIG 1033
            P+DSDKQQA +  I ++DGYNWGYNPVLWGVPKGSYA NPNG SRI++FRKMIQA+N IG
Sbjct: 413  PADSDKQQAEVIGIQNEDGYNWGYNPVLWGVPKGSYAGNPNGPSRILEFRKMIQAVNRIG 472

Query: 1034 LRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLMVERL 1213
            LRVVLDVVYNH+ +SGPL+ +SVLDKVVPGYY+RRN DG IENS C+NNTASEH MVERL
Sbjct: 473  LRVVLDVVYNHLASSGPLNENSVLDKVVPGYYLRRNTDGAIENSACMNNTASEHFMVERL 532

Query: 1214 IIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYGEGWD 1393
            I+DDLL WAV YKVDGFRFDLMGHIMKSTM+KA+S LQSLSKD++GVDG KIYIYGEGWD
Sbjct: 533  ILDDLLNWAVVYKVDGFRFDLMGHIMKSTMVKARSTLQSLSKDKDGVDGPKIYIYGEGWD 592

Query: 1394 FGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQPNAYD 1573
            FGE+AKNKRGINASQFNI GTGIGSFNDR+RDAVLGGSPFGHPLQQGFVTGLSL+PN YD
Sbjct: 593  FGEIAKNKRGINASQFNISGTGIGSFNDRMRDAVLGGSPFGHPLQQGFVTGLSLEPNGYD 652

Query: 1574 YGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYALCPIE 1753
            +G K+VTD MLS+S DHIQVGLAANL DFVLT H G+A+KGSE+ THDG+PVGYAL P E
Sbjct: 653  HGDKSVTDRMLSSSADHIQVGLAANLRDFVLTNHEGKAMKGSEVLTHDGVPVGYALSPTE 712

Query: 1754 TVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEILRS 1930
            TVNYVSAHDNETLFD + LKT  E+ VDDRCR+NHLA+S+VALSQG+PFFHSGDE+LRS
Sbjct: 713  TVNYVSAHDNETLFDIVSLKTVLELCVDDRCRMNHLASSLVALSQGIPFFHSGDELLRS 771



 Score =  139 bits (350), Expect = 2e-30
 Identities = 68/103 (66%), Positives = 81/103 (78%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           +VK  LEP   GLP NV +KFPHI +Y AF +  SVD  +L+KCQLA+ +F V+GKC NA
Sbjct: 131 DVKILLEPEYYGLPANVTEKFPHIVNYSAFKISESVDFENLLKCQLAI-AFYVDGKCKNA 189

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 309
           TGLQLPGVLDD+FAY GPLGAVFS+EA+TL+LWAPTAQ    C
Sbjct: 190 TGLQLPGVLDDLFAYNGPLGAVFSKEAVTLHLWAPTAQEVSVC 232


>ref|XP_010267261.1| PREDICTED: pullulanase 1, chloroplastic isoform X4 [Nelumbo nucifera]
          Length = 854

 Score =  900 bits (2325), Expect = 0.0
 Identities = 430/543 (79%), Positives = 478/543 (88%)
 Frame = +2

Query: 302  VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 481
            V A +Y D SGG  L+SVQL+E+ GVWSA GPR+WEGCYYVYEVSVYHPST +IETC  N
Sbjct: 223  VSAIIYGDSSGGVPLESVQLEEVDGVWSAHGPRNWEGCYYVYEVSVYHPSTLRIETCMAN 282

Query: 482  DPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDE 661
            DPYARG+SSDG RTLLVNLDC+ LKP GWD LA+EKP L SFSDISIYELH+RDFSASD+
Sbjct: 283  DPYARGLSSDGKRTLLVNLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQ 342

Query: 662  TVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQE 841
            TV+PDF GG+LAFT +DSAGVLHLKKL+ AGLTHVHLLP FQF GVDD++ KWK +D   
Sbjct: 343  TVHPDFHGGYLAFTLQDSAGVLHLKKLAYAGLTHVHLLPTFQFAGVDDEK-KWKHVDTDM 401

Query: 842  LAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQAL 1021
            LA LP DSD+QQA +T I D DGYNWGYNPVLWGVPKGSYASNPNG  R ++FRKM+QAL
Sbjct: 402  LATLPPDSDQQQARVTEIQDDDGYNWGYNPVLWGVPKGSYASNPNGSCRTVEFRKMVQAL 461

Query: 1022 NGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLM 1201
            N IGLRV+LDVVYNH+H SGPLD  SVLDKVVPGYY+RRN DG IENSTCINNTASEH M
Sbjct: 462  NRIGLRVILDVVYNHLHGSGPLDDYSVLDKVVPGYYLRRNSDGCIENSTCINNTASEHFM 521

Query: 1202 VERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYG 1381
            VERLI+DDLLCWAV+YKVDGFRFDLMGHIMKSTM+KAK+ALQSLSK++NGVDGS IYIYG
Sbjct: 522  VERLILDDLLCWAVDYKVDGFRFDLMGHIMKSTMVKAKNALQSLSKEKNGVDGSTIYIYG 581

Query: 1382 EGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 1561
            EGWDFGEVAKN RGINASQFN+CG GIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP
Sbjct: 582  EGWDFGEVAKNGRGINASQFNLCGIGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 641

Query: 1562 NAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYAL 1741
            N++D+G K   D ML+ S D+IQVG+AANL DFVLT H G  VKGSE+ THD +PV YAL
Sbjct: 642  NSHDHGDKTSMDHMLAVSQDYIQVGMAANLRDFVLTNHEGVEVKGSEVLTHDQLPVAYAL 701

Query: 1742 CPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEI 1921
            CPIET+NYVSAHDNETLFD + +KT  EISVD+RCR+NHLATSIVALSQG+PFFHSGDE+
Sbjct: 702  CPIETINYVSAHDNETLFDIVSMKTPVEISVDERCRINHLATSIVALSQGIPFFHSGDEL 761

Query: 1922 LRS 1930
            LRS
Sbjct: 762  LRS 764



 Score =  145 bits (365), Expect = 2e-32
 Identities = 69/98 (70%), Positives = 81/98 (82%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           EVK +LE    GLP NVV+KFPHI+  + F VP SVDV  LVKCQLAV SF  +G+C NA
Sbjct: 124 EVKIKLEVENSGLPSNVVEKFPHIRHNRVFRVPTSVDVKSLVKCQLAVASFHSDGQCSNA 183

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQ 294
           TGLQLPGV+DD+FAYGGPLGAVF++EA++LYLWAPTAQ
Sbjct: 184 TGLQLPGVIDDLFAYGGPLGAVFTKEAVSLYLWAPTAQ 221


>ref|XP_010267260.1| PREDICTED: pullulanase 1, chloroplastic isoform X3 [Nelumbo nucifera]
          Length = 932

 Score =  900 bits (2325), Expect = 0.0
 Identities = 430/543 (79%), Positives = 478/543 (88%)
 Frame = +2

Query: 302  VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 481
            V A +Y D SGG  L+SVQL+E+ GVWSA GPR+WEGCYYVYEVSVYHPST +IETC  N
Sbjct: 223  VSAIIYGDSSGGVPLESVQLEEVDGVWSAHGPRNWEGCYYVYEVSVYHPSTLRIETCMAN 282

Query: 482  DPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDE 661
            DPYARG+SSDG RTLLVNLDC+ LKP GWD LA+EKP L SFSDISIYELH+RDFSASD+
Sbjct: 283  DPYARGLSSDGKRTLLVNLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQ 342

Query: 662  TVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQE 841
            TV+PDF GG+LAFT +DSAGVLHLKKL+ AGLTHVHLLP FQF GVDD++ KWK +D   
Sbjct: 343  TVHPDFHGGYLAFTLQDSAGVLHLKKLAYAGLTHVHLLPTFQFAGVDDEK-KWKHVDTDM 401

Query: 842  LAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQAL 1021
            LA LP DSD+QQA +T I D DGYNWGYNPVLWGVPKGSYASNPNG  R ++FRKM+QAL
Sbjct: 402  LATLPPDSDQQQARVTEIQDDDGYNWGYNPVLWGVPKGSYASNPNGSCRTVEFRKMVQAL 461

Query: 1022 NGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLM 1201
            N IGLRV+LDVVYNH+H SGPLD  SVLDKVVPGYY+RRN DG IENSTCINNTASEH M
Sbjct: 462  NRIGLRVILDVVYNHLHGSGPLDDYSVLDKVVPGYYLRRNSDGCIENSTCINNTASEHFM 521

Query: 1202 VERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYG 1381
            VERLI+DDLLCWAV+YKVDGFRFDLMGHIMKSTM+KAK+ALQSLSK++NGVDGS IYIYG
Sbjct: 522  VERLILDDLLCWAVDYKVDGFRFDLMGHIMKSTMVKAKNALQSLSKEKNGVDGSTIYIYG 581

Query: 1382 EGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 1561
            EGWDFGEVAKN RGINASQFN+CG GIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP
Sbjct: 582  EGWDFGEVAKNGRGINASQFNLCGIGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 641

Query: 1562 NAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYAL 1741
            N++D+G K   D ML+ S D+IQVG+AANL DFVLT H G  VKGSE+ THD +PV YAL
Sbjct: 642  NSHDHGDKTSMDHMLAVSQDYIQVGMAANLRDFVLTNHEGVEVKGSEVLTHDQLPVAYAL 701

Query: 1742 CPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEI 1921
            CPIET+NYVSAHDNETLFD + +KT  EISVD+RCR+NHLATSIVALSQG+PFFHSGDE+
Sbjct: 702  CPIETINYVSAHDNETLFDIVSMKTPVEISVDERCRINHLATSIVALSQGIPFFHSGDEL 761

Query: 1922 LRS 1930
            LRS
Sbjct: 762  LRS 764



 Score =  145 bits (365), Expect = 2e-32
 Identities = 69/98 (70%), Positives = 81/98 (82%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           EVK +LE    GLP NVV+KFPHI+  + F VP SVDV  LVKCQLAV SF  +G+C NA
Sbjct: 124 EVKIKLEVENSGLPSNVVEKFPHIRHNRVFRVPTSVDVKSLVKCQLAVASFHSDGQCSNA 183

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQ 294
           TGLQLPGV+DD+FAYGGPLGAVF++EA++LYLWAPTAQ
Sbjct: 184 TGLQLPGVIDDLFAYGGPLGAVFTKEAVSLYLWAPTAQ 221


>ref|XP_010267258.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nelumbo nucifera]
          Length = 958

 Score =  900 bits (2325), Expect = 0.0
 Identities = 430/543 (79%), Positives = 478/543 (88%)
 Frame = +2

Query: 302  VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 481
            V A +Y D SGG  L+SVQL+E+ GVWSA GPR+WEGCYYVYEVSVYHPST +IETC  N
Sbjct: 223  VSAIIYGDSSGGVPLESVQLEEVDGVWSAHGPRNWEGCYYVYEVSVYHPSTLRIETCMAN 282

Query: 482  DPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDE 661
            DPYARG+SSDG RTLLVNLDC+ LKP GWD LA+EKP L SFSDISIYELH+RDFSASD+
Sbjct: 283  DPYARGLSSDGKRTLLVNLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQ 342

Query: 662  TVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQE 841
            TV+PDF GG+LAFT +DSAGVLHLKKL+ AGLTHVHLLP FQF GVDD++ KWK +D   
Sbjct: 343  TVHPDFHGGYLAFTLQDSAGVLHLKKLAYAGLTHVHLLPTFQFAGVDDEK-KWKHVDTDM 401

Query: 842  LAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQAL 1021
            LA LP DSD+QQA +T I D DGYNWGYNPVLWGVPKGSYASNPNG  R ++FRKM+QAL
Sbjct: 402  LATLPPDSDQQQARVTEIQDDDGYNWGYNPVLWGVPKGSYASNPNGSCRTVEFRKMVQAL 461

Query: 1022 NGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLM 1201
            N IGLRV+LDVVYNH+H SGPLD  SVLDKVVPGYY+RRN DG IENSTCINNTASEH M
Sbjct: 462  NRIGLRVILDVVYNHLHGSGPLDDYSVLDKVVPGYYLRRNSDGCIENSTCINNTASEHFM 521

Query: 1202 VERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYG 1381
            VERLI+DDLLCWAV+YKVDGFRFDLMGHIMKSTM+KAK+ALQSLSK++NGVDGS IYIYG
Sbjct: 522  VERLILDDLLCWAVDYKVDGFRFDLMGHIMKSTMVKAKNALQSLSKEKNGVDGSTIYIYG 581

Query: 1382 EGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 1561
            EGWDFGEVAKN RGINASQFN+CG GIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP
Sbjct: 582  EGWDFGEVAKNGRGINASQFNLCGIGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 641

Query: 1562 NAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYAL 1741
            N++D+G K   D ML+ S D+IQVG+AANL DFVLT H G  VKGSE+ THD +PV YAL
Sbjct: 642  NSHDHGDKTSMDHMLAVSQDYIQVGMAANLRDFVLTNHEGVEVKGSEVLTHDQLPVAYAL 701

Query: 1742 CPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEI 1921
            CPIET+NYVSAHDNETLFD + +KT  EISVD+RCR+NHLATSIVALSQG+PFFHSGDE+
Sbjct: 702  CPIETINYVSAHDNETLFDIVSMKTPVEISVDERCRINHLATSIVALSQGIPFFHSGDEL 761

Query: 1922 LRS 1930
            LRS
Sbjct: 762  LRS 764



 Score =  145 bits (365), Expect = 2e-32
 Identities = 69/98 (70%), Positives = 81/98 (82%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           EVK +LE    GLP NVV+KFPHI+  + F VP SVDV  LVKCQLAV SF  +G+C NA
Sbjct: 124 EVKIKLEVENSGLPSNVVEKFPHIRHNRVFRVPTSVDVKSLVKCQLAVASFHSDGQCSNA 183

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQ 294
           TGLQLPGV+DD+FAYGGPLGAVF++EA++LYLWAPTAQ
Sbjct: 184 TGLQLPGVIDDLFAYGGPLGAVFTKEAVSLYLWAPTAQ 221


>gb|OVA17964.1| Glycoside hydrolase [Macleaya cordata]
          Length = 983

 Score =  889 bits (2298), Expect = 0.0
 Identities = 415/544 (76%), Positives = 477/544 (87%)
 Frame = +2

Query: 299  VVHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKV 478
            VV A +Y++PSGG  ++ ++L+E++GVWSA G RSWEGCYYVYEVSVYHPSTS +ETC  
Sbjct: 246  VVRACIYREPSGGSPIEIIRLEEVNGVWSAHGSRSWEGCYYVYEVSVYHPSTSLVETCIA 305

Query: 479  NDPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASD 658
            NDPYARG+SSDG RTLLVNLDC+ LKP GW++L ++KP+  SFSD+SIYELH+RDFSA+D
Sbjct: 306  NDPYARGLSSDGKRTLLVNLDCDSLKPEGWEKLVDQKPEFSSFSDVSIYELHIRDFSAND 365

Query: 659  ETVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQ 838
             TVNPDF+GG+LAFTS+DSAG+ HLKKL +AGLTH+HLLP FQF  V D+++KWK LD  
Sbjct: 366  HTVNPDFQGGYLAFTSQDSAGIRHLKKLHNAGLTHLHLLPTFQFADVADEKEKWKYLDTD 425

Query: 839  ELAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQA 1018
            +LA LP DSD+QQA +T+I D+DGYNWGYNPVLWGVPKGSYASNPNG  R ++FRKM+Q+
Sbjct: 426  KLATLPPDSDEQQAQVTAIQDEDGYNWGYNPVLWGVPKGSYASNPNGSCRTLEFRKMVQS 485

Query: 1019 LNGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHL 1198
            LN IGLRVVLDVVYNH+H +GP   +SVLDKVVPGYY+RRN DG IE+STC+NNTASEH 
Sbjct: 486  LNRIGLRVVLDVVYNHLHGNGPHGDNSVLDKVVPGYYLRRNSDGCIEHSTCVNNTASEHF 545

Query: 1199 MVERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIY 1378
            M ERLI+DD+LCWAV+YKVDGFRFDLMGHIMK TMLKA SAL SLSKD+NGVDG KIYIY
Sbjct: 546  MAERLILDDILCWAVDYKVDGFRFDLMGHIMKRTMLKAYSALHSLSKDQNGVDGPKIYIY 605

Query: 1379 GEGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQ 1558
            GEGWDFGEVAKN RG+NASQFN+CGTGIGSFNDR+RDAVLGGSPFGHPLQQGFVTGLS Q
Sbjct: 606  GEGWDFGEVAKNGRGVNASQFNLCGTGIGSFNDRMRDAVLGGSPFGHPLQQGFVTGLSFQ 665

Query: 1559 PNAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYA 1738
            PN +D+G K V D ML+AS DHIQV +A NL DFVLT H GE VKGSE+ THDG+PV YA
Sbjct: 666  PNGHDHGVKIVVDSMLAASQDHIQVAMAGNLKDFVLTSHLGEEVKGSEVLTHDGLPVAYA 725

Query: 1739 LCPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDE 1918
            LCPIETVNYVSAHDNETLFD I LKT  EISVD+RCR+NHLATSI+ALSQGVPFFH GDE
Sbjct: 726  LCPIETVNYVSAHDNETLFDIISLKTPMEISVDERCRLNHLATSIIALSQGVPFFHCGDE 785

Query: 1919 ILRS 1930
            +LRS
Sbjct: 786  MLRS 789



 Score =  143 bits (361), Expect = 7e-32
 Identities = 65/103 (63%), Positives = 84/103 (81%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           +VK +LEP   GLP NV++KFPHI++Y  F VP  +D  +L+KCQLAVV+F  +G+C +A
Sbjct: 148 DVKIKLEPDHSGLPVNVIEKFPHIQNYNVFKVPFGLDAKNLLKCQLAVVAFKADGQCSDA 207

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 309
           TGLQLPGVLDD+F+Y GPLGAVFS+EA++LY+WAPTAQV   C
Sbjct: 208 TGLQLPGVLDDLFSYVGPLGAVFSKEAVSLYIWAPTAQVVRAC 250


>ref|XP_020084106.1| pullulanase 1, chloroplastic [Ananas comosus]
          Length = 986

 Score =  885 bits (2286), Expect = 0.0
 Identities = 425/543 (78%), Positives = 479/543 (88%)
 Frame = +2

Query: 302  VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 481
            V A  Y+DPSGG  L+ +QL+EL+GVWS VGPRSW G YYVYE+SVYHPSTS+IE C+ N
Sbjct: 249  VSACFYEDPSGGVPLECIQLKELNGVWSVVGPRSWAGRYYVYELSVYHPSTSRIEKCESN 308

Query: 482  DPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDE 661
            DPYARG+SSDG RT LV+LDCE LKP GWDELA++KP L SFSDISIYELH+RDFSASD 
Sbjct: 309  DPYARGLSSDGKRTWLVDLDCEDLKPAGWDELADQKPSLHSFSDISIYELHIRDFSASDH 368

Query: 662  TVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQE 841
            TV+PD RGG+ AFTS+DSAGV HLKKLSDAGLTHVHLLP F FGGVDD +  WK LD   
Sbjct: 369  TVDPDSRGGYRAFTSQDSAGVSHLKKLSDAGLTHVHLLPSFHFGGVDDIQSNWKYLDEAA 428

Query: 842  LAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQAL 1021
            +AKLP DSD+QQA +T++ D+DGYNWGYNPVLWGVPKGSYASNPNG SRI+++R+MIQAL
Sbjct: 429  MAKLPPDSDEQQAQVTAVQDEDGYNWGYNPVLWGVPKGSYASNPNGPSRILEYRQMIQAL 488

Query: 1022 NGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLM 1201
            N IGLRVVLDVVYNH+  +GP + +SVLDKVVPGYY+RRN DG IENSTC+NNTASEH M
Sbjct: 489  NRIGLRVVLDVVYNHLSENGPFNVNSVLDKVVPGYYLRRNADGGIENSTCMNNTASEHFM 548

Query: 1202 VERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYG 1381
            VERLI+DDLL WAV YKVDGFRFDLMGHIMKSTM+KAKS LQSL+KD+NGVDG+KIYIYG
Sbjct: 549  VERLILDDLLYWAVAYKVDGFRFDLMGHIMKSTMVKAKSLLQSLTKDKNGVDGTKIYIYG 608

Query: 1382 EGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 1561
            EGWDFGEVA+N+RGINASQ NI GTGIGSFNDRIRDAVLGGSPFG+PLQQGFVTGLSL+P
Sbjct: 609  EGWDFGEVARNQRGINASQLNIGGTGIGSFNDRIRDAVLGGSPFGNPLQQGFVTGLSLEP 668

Query: 1562 NAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYAL 1741
            N YD G K+VTD MLS+S DHIQVGLA NL DFVLT H G  +KGSE+ T+DGMPVGYAL
Sbjct: 669  NGYDQGDKSVTDRMLSSSADHIQVGLAGNLRDFVLTCHTGLPMKGSEVLTNDGMPVGYAL 728

Query: 1742 CPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEI 1921
             P+ETVNYVSAHDNETLFD + LKT  E+SVDDRCR+NHLA+SI+ALSQGVPFFH+GDEI
Sbjct: 729  SPMETVNYVSAHDNETLFDIVSLKTPVELSVDDRCRINHLASSIIALSQGVPFFHAGDEI 788

Query: 1922 LRS 1930
            LRS
Sbjct: 789  LRS 791



 Score =  153 bits (387), Expect = 4e-35
 Identities = 76/103 (73%), Positives = 83/103 (80%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           +VK QLEP  C LP NV +KFPHIKDY+AF VP+S DV +L+K QLAV SF  NGKC NA
Sbjct: 151 DVKIQLEPDHCALPANVTEKFPHIKDYRAFKVPNS-DVQNLLKSQLAVASFYANGKCKNA 209

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 309
           TGLQLPGVLDDMFAY GPLGAVFS+EAI LYLWAPTAQ    C
Sbjct: 210 TGLQLPGVLDDMFAYNGPLGAVFSKEAIALYLWAPTAQDVSAC 252


>ref|XP_021295296.1| pullulanase 1, chloroplastic isoform X3 [Herrania umbratica]
          Length = 877

 Score =  875 bits (2261), Expect = 0.0
 Identities = 410/543 (75%), Positives = 472/543 (86%)
 Frame = +2

Query: 302  VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 481
            VHAH+YKDP GG  L+ +QL E +GVWS  GP+ WEGCYYVYEVSVYHPST QIE C  N
Sbjct: 223  VHAHIYKDPVGGSPLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYAN 282

Query: 482  DPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDE 661
            DPYARG+SSDG RTL VNLD   LKP GWDELA++KPD+ SFSDISIYELH+RDFSA+D+
Sbjct: 283  DPYARGLSSDGRRTLFVNLDSNSLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDK 342

Query: 662  TVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQE 841
            TVNPDF GG++AFT +DSAGVLHLKKLS+AG+THVHLLP FQF GVDD+ + WK +D++ 
Sbjct: 343  TVNPDFCGGYMAFTLQDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDESENWKYVDYKI 402

Query: 842  LAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQAL 1021
            L KLP DS +QQ  IT+I + D YNWGYNPVLWGVPKGSYAS+PNG  RII+FRKMIQAL
Sbjct: 403  LKKLPPDSAEQQVQITAIQNDDAYNWGYNPVLWGVPKGSYASDPNGPCRIIEFRKMIQAL 462

Query: 1022 NGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLM 1201
            N IGLR+VLDVVYNH+HASGP D DSVLDK+VPGYY+RRN DGFIENSTC+NNTASEH M
Sbjct: 463  NHIGLRIVLDVVYNHLHASGPFDKDSVLDKIVPGYYLRRNNDGFIENSTCVNNTASEHYM 522

Query: 1202 VERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYG 1381
            VERLIIDDLL W++ YKVDGFRFDLMGHIMKSTM+KAK AL  L+K+ NGVDGS+IYIYG
Sbjct: 523  VERLIIDDLLSWSINYKVDGFRFDLMGHIMKSTMVKAKDALWRLTKERNGVDGSRIYIYG 582

Query: 1382 EGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 1561
            EGWDFGEVA+N RGINASQFNICGTGIGSFNDRIRDA+LGGSPFGHPLQQGF+TGL L+P
Sbjct: 583  EGWDFGEVAENGRGINASQFNICGTGIGSFNDRIRDAMLGGSPFGHPLQQGFITGLFLEP 642

Query: 1562 NAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYAL 1741
            N +D+G+KAV  +ML+++ DHIQVG+AANL DFVLT   G+  KGSE++TH G PV YAL
Sbjct: 643  NGHDHGTKAVEREMLASAKDHIQVGMAANLRDFVLTDFEGKERKGSEVFTHGGTPVAYAL 702

Query: 1742 CPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEI 1921
             P ETVNYVSAHDNETLFD + LKT  EISV++RCR+NH AT+I+ALSQG+PFFH+GDE+
Sbjct: 703  LPTETVNYVSAHDNETLFDIVSLKTPVEISVEERCRINHFATTIIALSQGIPFFHAGDEM 762

Query: 1922 LRS 1930
            LRS
Sbjct: 763  LRS 765



 Score =  134 bits (338), Expect = 4e-29
 Identities = 59/98 (60%), Positives = 79/98 (80%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           + + +LE    GLP NV++KFPHI++Y+AF VP ++D   L+KCQLAV +F  +GKC NA
Sbjct: 124 DAEIKLEEDSTGLPPNVIEKFPHIQNYRAFKVPPALDTKSLLKCQLAVAAFNSHGKCSNA 183

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQ 294
           TGLQLPG+LD++F+Y GPLGA + EEA++LYLWAPTAQ
Sbjct: 184 TGLQLPGILDELFSYDGPLGAFYLEEAVSLYLWAPTAQ 221


>ref|XP_020703866.1| pullulanase 1, chloroplastic [Dendrobium catenatum]
          Length = 966

 Score =  877 bits (2267), Expect = 0.0
 Identities = 413/544 (75%), Positives = 476/544 (87%)
 Frame = +2

Query: 299  VVHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKV 478
            VVHA +YK PS GD  + + L++  GVW AVGPRSWEGCYY+Y++SVYH ST ++ET  V
Sbjct: 229  VVHALLYKCPSAGDPFECIHLKDQDGVWCAVGPRSWEGCYYLYQISVYHQSTVRVETYTV 288

Query: 479  NDPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASD 658
            +DPYARGISS+G RTLLV+L+ E LKP+GWD+LA EKPDL SFSDISIYELH+RDFS +D
Sbjct: 289  SDPYARGISSNGSRTLLVDLNSEDLKPSGWDDLAYEKPDLPSFSDISIYELHIRDFSEND 348

Query: 659  ETVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQ 838
            ++V+PDFRGG+LAFT +DS G+ HLKKLS+AGLTH+HLLPCFQF GVDDD+ +WK  D  
Sbjct: 349  QSVDPDFRGGYLAFTCQDSLGINHLKKLSNAGLTHLHLLPCFQFAGVDDDKSQWKHTDDV 408

Query: 839  ELAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQA 1018
             ++KLP+DS++QQAIITSI D+DGYNWGYNPVLWGVPKGSYASNPNG  RI++FRKMIQA
Sbjct: 409  MMSKLPADSEQQQAIITSIQDEDGYNWGYNPVLWGVPKGSYASNPNGPCRILEFRKMIQA 468

Query: 1019 LNGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHL 1198
            LN IGLRVVLDVVYNHVHASGPLD  S+LDKVVPGYY+RR I+G IE+STC+NNTASEH 
Sbjct: 469  LNHIGLRVVLDVVYNHVHASGPLDETSILDKVVPGYYLRRTIEGCIEHSTCMNNTASEHF 528

Query: 1199 MVERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIY 1378
            MVERLIIDDLL WA  YKVDGFRFDLMGH+MK TML+AK+A+++L+KD +GVDGSKIY+Y
Sbjct: 529  MVERLIIDDLLYWATAYKVDGFRFDLMGHLMKGTMLRAKAAIENLTKDVDGVDGSKIYLY 588

Query: 1379 GEGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQ 1558
            GEGWDFGEVAKNKRGINASQ NI G GIGSFNDR+RDA+LGGSPFGHPLQQGFVTGL LQ
Sbjct: 589  GEGWDFGEVAKNKRGINASQLNITGNGIGSFNDRLRDAILGGSPFGHPLQQGFVTGLLLQ 648

Query: 1559 PNAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYA 1738
            PN YD+G K + D MLS S DHIQVGLAANL +FVLT H+G+ +KG EI THDGMPVGYA
Sbjct: 649  PNEYDHGDKHIVDGMLSTSADHIQVGLAANLRNFVLTSHKGDVMKGCEILTHDGMPVGYA 708

Query: 1739 LCPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDE 1918
            L P ETVNYVSAHDNETLFD ICLK    ISVDDRCR+NH+ATSIVALSQG+PFFH GD+
Sbjct: 709  LSPTETVNYVSAHDNETLFDIICLKAPVGISVDDRCRINHVATSIVALSQGIPFFHCGDD 768

Query: 1919 ILRS 1930
            +LRS
Sbjct: 769  MLRS 772



 Score =  135 bits (339), Expect = 4e-29
 Identities = 63/99 (63%), Positives = 78/99 (78%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           + K  LE    GLP  V++KFPHI+DYKA+ VP SVD   ++KCQLA+ SFG +GKC + 
Sbjct: 131 DFKIPLEWERFGLPSKVLEKFPHIRDYKAYTVPASVDFRKILKCQLAIASFGDDGKCSDV 190

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQV 297
           TG+QLPGVLDD+FAY GPLGA+FS+ +ITL LWAPTAQV
Sbjct: 191 TGIQLPGVLDDLFAYDGPLGAIFSKRSITLNLWAPTAQV 229


>ref|XP_021295295.1| pullulanase 1, chloroplastic isoform X2 [Herrania umbratica]
          Length = 963

 Score =  875 bits (2261), Expect = 0.0
 Identities = 410/543 (75%), Positives = 472/543 (86%)
 Frame = +2

Query: 302  VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 481
            VHAH+YKDP GG  L+ +QL E +GVWS  GP+ WEGCYYVYEVSVYHPST QIE C  N
Sbjct: 221  VHAHIYKDPVGGSPLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYAN 280

Query: 482  DPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDE 661
            DPYARG+SSDG RTL VNLD   LKP GWDELA++KPD+ SFSDISIYELH+RDFSA+D+
Sbjct: 281  DPYARGLSSDGRRTLFVNLDSNSLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDK 340

Query: 662  TVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQE 841
            TVNPDF GG++AFT +DSAGVLHLKKLS+AG+THVHLLP FQF GVDD+ + WK +D++ 
Sbjct: 341  TVNPDFCGGYMAFTLQDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDESENWKYVDYKI 400

Query: 842  LAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQAL 1021
            L KLP DS +QQ  IT+I + D YNWGYNPVLWGVPKGSYAS+PNG  RII+FRKMIQAL
Sbjct: 401  LKKLPPDSAEQQVQITAIQNDDAYNWGYNPVLWGVPKGSYASDPNGPCRIIEFRKMIQAL 460

Query: 1022 NGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLM 1201
            N IGLR+VLDVVYNH+HASGP D DSVLDK+VPGYY+RRN DGFIENSTC+NNTASEH M
Sbjct: 461  NHIGLRIVLDVVYNHLHASGPFDKDSVLDKIVPGYYLRRNNDGFIENSTCVNNTASEHYM 520

Query: 1202 VERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYG 1381
            VERLIIDDLL W++ YKVDGFRFDLMGHIMKSTM+KAK AL  L+K+ NGVDGS+IYIYG
Sbjct: 521  VERLIIDDLLSWSINYKVDGFRFDLMGHIMKSTMVKAKDALWRLTKERNGVDGSRIYIYG 580

Query: 1382 EGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 1561
            EGWDFGEVA+N RGINASQFNICGTGIGSFNDRIRDA+LGGSPFGHPLQQGF+TGL L+P
Sbjct: 581  EGWDFGEVAENGRGINASQFNICGTGIGSFNDRIRDAMLGGSPFGHPLQQGFITGLFLEP 640

Query: 1562 NAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYAL 1741
            N +D+G+KAV  +ML+++ DHIQVG+AANL DFVLT   G+  KGSE++TH G PV YAL
Sbjct: 641  NGHDHGTKAVEREMLASAKDHIQVGMAANLRDFVLTDFEGKERKGSEVFTHGGTPVAYAL 700

Query: 1742 CPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEI 1921
             P ETVNYVSAHDNETLFD + LKT  EISV++RCR+NH AT+I+ALSQG+PFFH+GDE+
Sbjct: 701  LPTETVNYVSAHDNETLFDIVSLKTPVEISVEERCRINHFATTIIALSQGIPFFHAGDEM 760

Query: 1922 LRS 1930
            LRS
Sbjct: 761  LRS 763



 Score =  134 bits (338), Expect = 5e-29
 Identities = 59/98 (60%), Positives = 79/98 (80%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           + + +LE    GLP NV++KFPHI++Y+AF VP ++D   L+KCQLAV +F  +GKC NA
Sbjct: 122 DAEIKLEEDSTGLPPNVIEKFPHIQNYRAFKVPPALDTKSLLKCQLAVAAFNSHGKCSNA 181

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQ 294
           TGLQLPG+LD++F+Y GPLGA + EEA++LYLWAPTAQ
Sbjct: 182 TGLQLPGILDELFSYDGPLGAFYLEEAVSLYLWAPTAQ 219


>ref|XP_021295293.1| pullulanase 1, chloroplastic isoform X1 [Herrania umbratica]
          Length = 965

 Score =  875 bits (2261), Expect = 0.0
 Identities = 410/543 (75%), Positives = 472/543 (86%)
 Frame = +2

Query: 302  VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 481
            VHAH+YKDP GG  L+ +QL E +GVWS  GP+ WEGCYYVYEVSVYHPST QIE C  N
Sbjct: 223  VHAHIYKDPVGGSPLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYAN 282

Query: 482  DPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDE 661
            DPYARG+SSDG RTL VNLD   LKP GWDELA++KPD+ SFSDISIYELH+RDFSA+D+
Sbjct: 283  DPYARGLSSDGRRTLFVNLDSNSLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDK 342

Query: 662  TVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQE 841
            TVNPDF GG++AFT +DSAGVLHLKKLS+AG+THVHLLP FQF GVDD+ + WK +D++ 
Sbjct: 343  TVNPDFCGGYMAFTLQDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDESENWKYVDYKI 402

Query: 842  LAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQAL 1021
            L KLP DS +QQ  IT+I + D YNWGYNPVLWGVPKGSYAS+PNG  RII+FRKMIQAL
Sbjct: 403  LKKLPPDSAEQQVQITAIQNDDAYNWGYNPVLWGVPKGSYASDPNGPCRIIEFRKMIQAL 462

Query: 1022 NGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLM 1201
            N IGLR+VLDVVYNH+HASGP D DSVLDK+VPGYY+RRN DGFIENSTC+NNTASEH M
Sbjct: 463  NHIGLRIVLDVVYNHLHASGPFDKDSVLDKIVPGYYLRRNNDGFIENSTCVNNTASEHYM 522

Query: 1202 VERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYG 1381
            VERLIIDDLL W++ YKVDGFRFDLMGHIMKSTM+KAK AL  L+K+ NGVDGS+IYIYG
Sbjct: 523  VERLIIDDLLSWSINYKVDGFRFDLMGHIMKSTMVKAKDALWRLTKERNGVDGSRIYIYG 582

Query: 1382 EGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 1561
            EGWDFGEVA+N RGINASQFNICGTGIGSFNDRIRDA+LGGSPFGHPLQQGF+TGL L+P
Sbjct: 583  EGWDFGEVAENGRGINASQFNICGTGIGSFNDRIRDAMLGGSPFGHPLQQGFITGLFLEP 642

Query: 1562 NAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYAL 1741
            N +D+G+KAV  +ML+++ DHIQVG+AANL DFVLT   G+  KGSE++TH G PV YAL
Sbjct: 643  NGHDHGTKAVEREMLASAKDHIQVGMAANLRDFVLTDFEGKERKGSEVFTHGGTPVAYAL 702

Query: 1742 CPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEI 1921
             P ETVNYVSAHDNETLFD + LKT  EISV++RCR+NH AT+I+ALSQG+PFFH+GDE+
Sbjct: 703  LPTETVNYVSAHDNETLFDIVSLKTPVEISVEERCRINHFATTIIALSQGIPFFHAGDEM 762

Query: 1922 LRS 1930
            LRS
Sbjct: 763  LRS 765



 Score =  134 bits (338), Expect = 5e-29
 Identities = 59/98 (60%), Positives = 79/98 (80%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           + + +LE    GLP NV++KFPHI++Y+AF VP ++D   L+KCQLAV +F  +GKC NA
Sbjct: 124 DAEIKLEEDSTGLPPNVIEKFPHIQNYRAFKVPPALDTKSLLKCQLAVAAFNSHGKCSNA 183

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQ 294
           TGLQLPG+LD++F+Y GPLGA + EEA++LYLWAPTAQ
Sbjct: 184 TGLQLPGILDELFSYDGPLGAFYLEEAVSLYLWAPTAQ 221


>ref|XP_017984815.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Theobroma cacao]
          Length = 963

 Score =  874 bits (2258), Expect = 0.0
 Identities = 411/543 (75%), Positives = 472/543 (86%)
 Frame = +2

Query: 302  VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 481
            VHAH+YKDP GG  L+ +QL E +GVWS  GP+ WEGCYYVYEVSVYHPST QIE C  N
Sbjct: 221  VHAHIYKDPMGGSPLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYAN 280

Query: 482  DPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDE 661
            DPYARG+SSDG RTL VNLD   LKP GWDELA++KPD+ SFSDISIYELH+RDFSA+D 
Sbjct: 281  DPYARGLSSDGRRTLFVNLDSNGLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDN 340

Query: 662  TVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQE 841
            TVNPDF GG++AFT +DSAGVLHLKKLS+AG+THVHLLP FQF GVDD+ + WK +D++ 
Sbjct: 341  TVNPDFCGGYMAFTLQDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDESENWKYVDYKI 400

Query: 842  LAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQAL 1021
            L KLP DS  QQA IT+I + D YNWGYNPVLWGVPKGSYAS+PNG  RII+FRKMIQAL
Sbjct: 401  LQKLPPDSAGQQAQITAIQNDDAYNWGYNPVLWGVPKGSYASDPNGPCRIIEFRKMIQAL 460

Query: 1022 NGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLM 1201
            N IGLR+VLDVVYNH+HASGP D DSVLDK+VPGY++RRN DGFIENSTC+NNTASEH M
Sbjct: 461  NHIGLRIVLDVVYNHLHASGPFDKDSVLDKIVPGYHLRRNNDGFIENSTCVNNTASEHYM 520

Query: 1202 VERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYG 1381
            VERLIIDDLL W++ YKVDGFRFDLMGHIMKSTM+KAK AL SL+K+ NGVDGS+IYIYG
Sbjct: 521  VERLIIDDLLSWSINYKVDGFRFDLMGHIMKSTMVKAKDALWSLTKERNGVDGSRIYIYG 580

Query: 1382 EGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 1561
            EGWDFGEVA+N RGINASQFNICGTGIGSF+DRIRDA+LGGSPFGHPLQQGF+TGL L+P
Sbjct: 581  EGWDFGEVAENGRGINASQFNICGTGIGSFDDRIRDAMLGGSPFGHPLQQGFITGLYLEP 640

Query: 1562 NAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYAL 1741
            N +D+G+KAV   ML+++ DHIQVG+AANL DFVLT   G+  KGSE++T+ G PV YAL
Sbjct: 641  NGHDHGTKAVERAMLASAKDHIQVGMAANLRDFVLTDFEGKERKGSEVFTYGGTPVAYAL 700

Query: 1742 CPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEI 1921
            CP ETVNYVSAHDNETLFD + LKT  EISV++RCR+NH ATSI+ALSQG+PFFH+GDE+
Sbjct: 701  CPTETVNYVSAHDNETLFDIVSLKTPVEISVEERCRINHFATSIIALSQGIPFFHAGDEM 760

Query: 1922 LRS 1930
            LRS
Sbjct: 761  LRS 763



 Score =  127 bits (319), Expect = 1e-26
 Identities = 56/98 (57%), Positives = 77/98 (78%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           + + +LE    GLP NV++KFPHI++Y+AF VP ++D  +L+KCQLAV +F  +G C NA
Sbjct: 122 DAEIKLEEDRNGLPPNVIEKFPHIQNYRAFKVPPALDAKNLLKCQLAVAAFNSHGNCSNA 181

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQ 294
           TGLQLPG+LD++F+Y GPLGA +  E ++LYLWAPTAQ
Sbjct: 182 TGLQLPGILDELFSYDGPLGAHYLGEVVSLYLWAPTAQ 219


>ref|XP_007010038.2| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Theobroma cacao]
          Length = 965

 Score =  874 bits (2258), Expect = 0.0
 Identities = 411/543 (75%), Positives = 472/543 (86%)
 Frame = +2

Query: 302  VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 481
            VHAH+YKDP GG  L+ +QL E +GVWS  GP+ WEGCYYVYEVSVYHPST QIE C  N
Sbjct: 223  VHAHIYKDPMGGSPLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYAN 282

Query: 482  DPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDE 661
            DPYARG+SSDG RTL VNLD   LKP GWDELA++KPD+ SFSDISIYELH+RDFSA+D 
Sbjct: 283  DPYARGLSSDGRRTLFVNLDSNGLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDN 342

Query: 662  TVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQE 841
            TVNPDF GG++AFT +DSAGVLHLKKLS+AG+THVHLLP FQF GVDD+ + WK +D++ 
Sbjct: 343  TVNPDFCGGYMAFTLQDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDESENWKYVDYKI 402

Query: 842  LAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQAL 1021
            L KLP DS  QQA IT+I + D YNWGYNPVLWGVPKGSYAS+PNG  RII+FRKMIQAL
Sbjct: 403  LQKLPPDSAGQQAQITAIQNDDAYNWGYNPVLWGVPKGSYASDPNGPCRIIEFRKMIQAL 462

Query: 1022 NGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLM 1201
            N IGLR+VLDVVYNH+HASGP D DSVLDK+VPGY++RRN DGFIENSTC+NNTASEH M
Sbjct: 463  NHIGLRIVLDVVYNHLHASGPFDKDSVLDKIVPGYHLRRNNDGFIENSTCVNNTASEHYM 522

Query: 1202 VERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYG 1381
            VERLIIDDLL W++ YKVDGFRFDLMGHIMKSTM+KAK AL SL+K+ NGVDGS+IYIYG
Sbjct: 523  VERLIIDDLLSWSINYKVDGFRFDLMGHIMKSTMVKAKDALWSLTKERNGVDGSRIYIYG 582

Query: 1382 EGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 1561
            EGWDFGEVA+N RGINASQFNICGTGIGSF+DRIRDA+LGGSPFGHPLQQGF+TGL L+P
Sbjct: 583  EGWDFGEVAENGRGINASQFNICGTGIGSFDDRIRDAMLGGSPFGHPLQQGFITGLYLEP 642

Query: 1562 NAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYAL 1741
            N +D+G+KAV   ML+++ DHIQVG+AANL DFVLT   G+  KGSE++T+ G PV YAL
Sbjct: 643  NGHDHGTKAVERAMLASAKDHIQVGMAANLRDFVLTDFEGKERKGSEVFTYGGTPVAYAL 702

Query: 1742 CPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEI 1921
            CP ETVNYVSAHDNETLFD + LKT  EISV++RCR+NH ATSI+ALSQG+PFFH+GDE+
Sbjct: 703  CPTETVNYVSAHDNETLFDIVSLKTPVEISVEERCRINHFATSIIALSQGIPFFHAGDEM 762

Query: 1922 LRS 1930
            LRS
Sbjct: 763  LRS 765



 Score =  127 bits (319), Expect = 1e-26
 Identities = 56/98 (57%), Positives = 77/98 (78%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           + + +LE    GLP NV++KFPHI++Y+AF VP ++D  +L+KCQLAV +F  +G C NA
Sbjct: 124 DAEIKLEEDRNGLPPNVIEKFPHIQNYRAFKVPPALDAKNLLKCQLAVAAFNSHGNCSNA 183

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQ 294
           TGLQLPG+LD++F+Y GPLGA +  E ++LYLWAPTAQ
Sbjct: 184 TGLQLPGILDELFSYDGPLGAHYLGEVVSLYLWAPTAQ 221


>gb|EOY18848.1| Limit dextrinase [Theobroma cacao]
          Length = 965

 Score =  874 bits (2258), Expect = 0.0
 Identities = 411/543 (75%), Positives = 472/543 (86%)
 Frame = +2

Query: 302  VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 481
            VHAH+YKDP GG  L+ +QL E +GVWS  GP+ WEGCYYVYEVSVYHPST QIE C  N
Sbjct: 223  VHAHIYKDPMGGSPLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYAN 282

Query: 482  DPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDE 661
            DPYARG+SSDG RTL VNLD   LKP GWDELA++KPD+ SFSDISIYELH+RDFSA+D 
Sbjct: 283  DPYARGLSSDGRRTLFVNLDSNGLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDN 342

Query: 662  TVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQE 841
            TVNPDF GG++AFT +DSAGVLHLKKLS+AG+THVHLLP FQF GVDD+ + WK +D++ 
Sbjct: 343  TVNPDFCGGYMAFTLQDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDESENWKYVDYKI 402

Query: 842  LAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQAL 1021
            L KLP DS  QQA IT+I + D YNWGYNPVLWGVPKGSYAS+PNG  RII+FRKMIQAL
Sbjct: 403  LKKLPPDSAGQQAQITAIQNDDAYNWGYNPVLWGVPKGSYASDPNGPCRIIEFRKMIQAL 462

Query: 1022 NGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLM 1201
            N IGLR+VLDVVYNH+HASGP D DSVLDK+VPGY++RRN DGFIENSTC+NNTASEH M
Sbjct: 463  NHIGLRIVLDVVYNHLHASGPFDKDSVLDKIVPGYHLRRNNDGFIENSTCVNNTASEHYM 522

Query: 1202 VERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYG 1381
            VERLIIDDLL W++ YKVDGFRFDLMGHIMKSTM+KAK AL SL+K+ NGVDGS+IYIYG
Sbjct: 523  VERLIIDDLLSWSINYKVDGFRFDLMGHIMKSTMVKAKDALWSLTKERNGVDGSRIYIYG 582

Query: 1382 EGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 1561
            EGWDFGEVA+N RGINASQFNICGTGIGSF+DRIRDA+LGGSPFGHPLQQGF+TGL L+P
Sbjct: 583  EGWDFGEVAENGRGINASQFNICGTGIGSFDDRIRDAMLGGSPFGHPLQQGFITGLFLEP 642

Query: 1562 NAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYAL 1741
            N +D+G+KAV   ML+++ DHIQVG+AANL DFVLT   G+  KGSE++T+ G PV YAL
Sbjct: 643  NGHDHGTKAVERAMLASAKDHIQVGMAANLRDFVLTDFEGKERKGSEVFTYGGTPVAYAL 702

Query: 1742 CPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEI 1921
            CP ETVNYVSAHDNETLFD + LKT  EISV++RCR+NH ATSI+ALSQG+PFFH+GDE+
Sbjct: 703  CPTETVNYVSAHDNETLFDIVSLKTPVEISVEERCRINHFATSIIALSQGIPFFHAGDEM 762

Query: 1922 LRS 1930
            LRS
Sbjct: 763  LRS 765



 Score =  129 bits (324), Expect = 3e-27
 Identities = 57/98 (58%), Positives = 78/98 (79%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           + + +LE    GLP NV++KFPHI++Y+AF VP ++D  +L+KCQLAV +F  +GKC NA
Sbjct: 124 DAEIKLEEDRNGLPPNVIEKFPHIQNYRAFKVPPALDAKNLLKCQLAVAAFNSHGKCSNA 183

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQ 294
           TGLQLPG+LD++F+Y GPLGA +  E ++LYLWAPTAQ
Sbjct: 184 TGLQLPGILDELFSYDGPLGAHYLGEVVSLYLWAPTAQ 221


>ref|XP_019079995.1| PREDICTED: pullulanase 1, chloroplastic isoform X3 [Vitis vinifera]
          Length = 865

 Score =  868 bits (2242), Expect = 0.0
 Identities = 408/544 (75%), Positives = 468/544 (86%)
 Frame = +2

Query: 299  VVHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKV 478
            VVHA +Y DPSGGD L+ V L+E +GVWSA GP+SWEGCYY YEVSVYHPST QIE C  
Sbjct: 219  VVHACIYGDPSGGDPLEIVCLEEFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTA 278

Query: 479  NDPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASD 658
            NDPYARG+S+D  RTLLVNLD + LKP GWD LA+EKP L SFSDISIYELH+RDFSASD
Sbjct: 279  NDPYARGLSADARRTLLVNLDSDDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASD 338

Query: 659  ETVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQ 838
             TV+PDFRGG+LAFTS+DSAG+LHLKKL +AG++H+HLLP FQF GVDD++DKWK +D  
Sbjct: 339  HTVHPDFRGGYLAFTSQDSAGILHLKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVDPN 398

Query: 839  ELAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQA 1018
             L  LP DS +QQA I +I D+DGYNWGYNPVLWGVPKGSYASNPN   R ++FRKM+QA
Sbjct: 399  ILETLPPDSVEQQAQIMTIQDEDGYNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMVQA 458

Query: 1019 LNGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHL 1198
            LN IG RVVLDVVYNH+H SGP D +SVLDK+VPGYY+RRN DG IE+S C+NNTASEH 
Sbjct: 459  LNRIGFRVVLDVVYNHLHGSGPFDENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASEHF 518

Query: 1199 MVERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIY 1378
            MVERLI+DDLLCWAV+YKVDGFRFDLMGHIMK TM+KAK+ L SLSKD +G+DGS IYIY
Sbjct: 519  MVERLILDDLLCWAVDYKVDGFRFDLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIYIY 578

Query: 1379 GEGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQ 1558
            GEGWDFGEVAKN RG+NASQFN+CGTGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL LQ
Sbjct: 579  GEGWDFGEVAKNGRGVNASQFNLCGTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQ 638

Query: 1559 PNAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYA 1738
            PN +D+G + V + ML+ S DHIQVG+AANL DFVLT   GE VKGSE+ T+DG PV Y 
Sbjct: 639  PNDHDHGGEEVAERMLAVSKDHIQVGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVAYT 698

Query: 1739 LCPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDE 1918
            +CP ET+NYVSAHDNETLFD + LKT  +ISV++RCR+NHLATSI+ALSQG+PFFHSGDE
Sbjct: 699  VCPTETINYVSAHDNETLFDIVSLKTPMQISVEERCRINHLATSIIALSQGIPFFHSGDE 758

Query: 1919 ILRS 1930
            +LRS
Sbjct: 759  MLRS 762



 Score =  137 bits (346), Expect = 4e-30
 Identities = 63/103 (61%), Positives = 80/103 (77%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           ++  QLE    GLP NV++KFP ++ YKAF VP +VD   L+KCQLAV +F  +G+C N 
Sbjct: 121 DMTIQLEEDNGGLPINVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNV 180

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 309
           TGLQLPGVLD++F+Y GPLGA+FS+EA+TLYLWAPTAQV   C
Sbjct: 181 TGLQLPGVLDELFSYEGPLGAIFSKEAVTLYLWAPTAQVVHAC 223


>ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Vitis vinifera]
          Length = 954

 Score =  868 bits (2242), Expect = 0.0
 Identities = 408/544 (75%), Positives = 468/544 (86%)
 Frame = +2

Query: 299  VVHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKV 478
            VVHA +Y DPSGGD L+ V L+E +GVWSA GP+SWEGCYY YEVSVYHPST QIE C  
Sbjct: 217  VVHACIYGDPSGGDPLEIVCLEEFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTA 276

Query: 479  NDPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASD 658
            NDPYARG+S+D  RTLLVNLD + LKP GWD LA+EKP L SFSDISIYELH+RDFSASD
Sbjct: 277  NDPYARGLSADARRTLLVNLDSDDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASD 336

Query: 659  ETVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQ 838
             TV+PDFRGG+LAFTS+DSAG+LHLKKL +AG++H+HLLP FQF GVDD++DKWK +D  
Sbjct: 337  HTVHPDFRGGYLAFTSQDSAGILHLKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVDPN 396

Query: 839  ELAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQA 1018
             L  LP DS +QQA I +I D+DGYNWGYNPVLWGVPKGSYASNPN   R ++FRKM+QA
Sbjct: 397  ILETLPPDSVEQQAQIMTIQDEDGYNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMVQA 456

Query: 1019 LNGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHL 1198
            LN IG RVVLDVVYNH+H SGP D +SVLDK+VPGYY+RRN DG IE+S C+NNTASEH 
Sbjct: 457  LNRIGFRVVLDVVYNHLHGSGPFDENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASEHF 516

Query: 1199 MVERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIY 1378
            MVERLI+DDLLCWAV+YKVDGFRFDLMGHIMK TM+KAK+ L SLSKD +G+DGS IYIY
Sbjct: 517  MVERLILDDLLCWAVDYKVDGFRFDLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIYIY 576

Query: 1379 GEGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQ 1558
            GEGWDFGEVAKN RG+NASQFN+CGTGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL LQ
Sbjct: 577  GEGWDFGEVAKNGRGVNASQFNLCGTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQ 636

Query: 1559 PNAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYA 1738
            PN +D+G + V + ML+ S DHIQVG+AANL DFVLT   GE VKGSE+ T+DG PV Y 
Sbjct: 637  PNDHDHGGEEVAERMLAVSKDHIQVGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVAYT 696

Query: 1739 LCPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDE 1918
            +CP ET+NYVSAHDNETLFD + LKT  +ISV++RCR+NHLATSI+ALSQG+PFFHSGDE
Sbjct: 697  VCPTETINYVSAHDNETLFDIVSLKTPMQISVEERCRINHLATSIIALSQGIPFFHSGDE 756

Query: 1919 ILRS 1930
            +LRS
Sbjct: 757  MLRS 760



 Score =  137 bits (346), Expect = 5e-30
 Identities = 63/103 (61%), Positives = 80/103 (77%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           ++  QLE    GLP NV++KFP ++ YKAF VP +VD   L+KCQLAV +F  +G+C N 
Sbjct: 119 DMTIQLEEDNGGLPINVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNV 178

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 309
           TGLQLPGVLD++F+Y GPLGA+FS+EA+TLYLWAPTAQV   C
Sbjct: 179 TGLQLPGVLDELFSYEGPLGAIFSKEAVTLYLWAPTAQVVHAC 221


>ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Vitis vinifera]
 emb|CBI31395.3| unnamed protein product, partial [Vitis vinifera]
          Length = 956

 Score =  868 bits (2242), Expect = 0.0
 Identities = 408/544 (75%), Positives = 468/544 (86%)
 Frame = +2

Query: 299  VVHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKV 478
            VVHA +Y DPSGGD L+ V L+E +GVWSA GP+SWEGCYY YEVSVYHPST QIE C  
Sbjct: 219  VVHACIYGDPSGGDPLEIVCLEEFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTA 278

Query: 479  NDPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASD 658
            NDPYARG+S+D  RTLLVNLD + LKP GWD LA+EKP L SFSDISIYELH+RDFSASD
Sbjct: 279  NDPYARGLSADARRTLLVNLDSDDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASD 338

Query: 659  ETVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQ 838
             TV+PDFRGG+LAFTS+DSAG+LHLKKL +AG++H+HLLP FQF GVDD++DKWK +D  
Sbjct: 339  HTVHPDFRGGYLAFTSQDSAGILHLKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVDPN 398

Query: 839  ELAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQA 1018
             L  LP DS +QQA I +I D+DGYNWGYNPVLWGVPKGSYASNPN   R ++FRKM+QA
Sbjct: 399  ILETLPPDSVEQQAQIMTIQDEDGYNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMVQA 458

Query: 1019 LNGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHL 1198
            LN IG RVVLDVVYNH+H SGP D +SVLDK+VPGYY+RRN DG IE+S C+NNTASEH 
Sbjct: 459  LNRIGFRVVLDVVYNHLHGSGPFDENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASEHF 518

Query: 1199 MVERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIY 1378
            MVERLI+DDLLCWAV+YKVDGFRFDLMGHIMK TM+KAK+ L SLSKD +G+DGS IYIY
Sbjct: 519  MVERLILDDLLCWAVDYKVDGFRFDLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIYIY 578

Query: 1379 GEGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQ 1558
            GEGWDFGEVAKN RG+NASQFN+CGTGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL LQ
Sbjct: 579  GEGWDFGEVAKNGRGVNASQFNLCGTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQ 638

Query: 1559 PNAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYA 1738
            PN +D+G + V + ML+ S DHIQVG+AANL DFVLT   GE VKGSE+ T+DG PV Y 
Sbjct: 639  PNDHDHGGEEVAERMLAVSKDHIQVGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVAYT 698

Query: 1739 LCPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDE 1918
            +CP ET+NYVSAHDNETLFD + LKT  +ISV++RCR+NHLATSI+ALSQG+PFFHSGDE
Sbjct: 699  VCPTETINYVSAHDNETLFDIVSLKTPMQISVEERCRINHLATSIIALSQGIPFFHSGDE 758

Query: 1919 ILRS 1930
            +LRS
Sbjct: 759  MLRS 762



 Score =  137 bits (346), Expect = 5e-30
 Identities = 63/103 (61%), Positives = 80/103 (77%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           ++  QLE    GLP NV++KFP ++ YKAF VP +VD   L+KCQLAV +F  +G+C N 
Sbjct: 121 DMTIQLEEDNGGLPINVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNV 180

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 309
           TGLQLPGVLD++F+Y GPLGA+FS+EA+TLYLWAPTAQV   C
Sbjct: 181 TGLQLPGVLDELFSYEGPLGAIFSKEAVTLYLWAPTAQVVHAC 223


>dbj|GAV67655.1| Alpha-amylase domain-containing protein/CBM_48 domain-containing
            protein/DUF3372 domain-containing protein, partial
            [Cephalotus follicularis]
          Length = 857

 Score =  863 bits (2230), Expect = 0.0
 Identities = 407/543 (74%), Positives = 469/543 (86%)
 Frame = +2

Query: 302  VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 481
            V A +Y+D +GGD  + VQLQE++GVWS  GP+SWEGCYYVYEVSVYHPST QIE C  N
Sbjct: 226  VRACIYRDATGGDPQEIVQLQEVNGVWSTKGPKSWEGCYYVYEVSVYHPSTMQIEKCYAN 285

Query: 482  DPYARGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDE 661
            DPYARG+SSDG RTLLVNLD + LKP GWD++A+EKPD+ SFSDISIYELH+RDFSA+D 
Sbjct: 286  DPYARGLSSDGRRTLLVNLDSDTLKPEGWDKVADEKPDIISFSDISIYELHIRDFSANDH 345

Query: 662  TVNPDFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQE 841
            TV+PDFRG ++AFT +DSAG+LHLKKLS+AG+THVHLLP F F GV D+++KWK +D + 
Sbjct: 346  TVHPDFRGCYMAFTLQDSAGMLHLKKLSNAGITHVHLLPTFHFAGVVDEKEKWKNVDAEI 405

Query: 842  LAKLPSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQAL 1021
            L KLP DS +QQA IT+I + DGYNWGYNPVLWGVPKGSYA+NPNG  R I+FRKM+QAL
Sbjct: 406  LEKLPPDSTEQQAQITAIQNDDGYNWGYNPVLWGVPKGSYATNPNGPCRTIEFRKMVQAL 465

Query: 1022 NGIGLRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLM 1201
            N IGLRVVLDVVYNH+H +GP D +SVLDK+VPGYY+RRN DGFIE+STC+NNTASEH M
Sbjct: 466  NRIGLRVVLDVVYNHLHGNGPFDENSVLDKIVPGYYLRRNTDGFIEHSTCVNNTASEHYM 525

Query: 1202 VERLIIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYG 1381
            VERLI+DDLLCWA+ YKVDGFRFDLMGHIMKSTM+KAK AL  L+K+ +GV GS IYIYG
Sbjct: 526  VERLILDDLLCWAINYKVDGFRFDLMGHIMKSTMMKAKGALHRLTKERDGVSGSCIYIYG 585

Query: 1382 EGWDFGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQP 1561
            EGWDFGEVAKN RG+NASQFN+CGT IGSFNDRIRDA+LGGSPFGHPLQQGFVTGL LQP
Sbjct: 586  EGWDFGEVAKNARGVNASQFNLCGTEIGSFNDRIRDAILGGSPFGHPLQQGFVTGLLLQP 645

Query: 1562 NAYDYGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYAL 1741
            N +D+GS AV   ML+ + DHI+VGLAANL DFVL    G+ VKGSEI T+DG PV YAL
Sbjct: 646  NGHDHGSDAVQRHMLTLAADHIKVGLAANLKDFVLKNCEGKEVKGSEILTYDGAPVAYAL 705

Query: 1742 CPIETVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEI 1921
            CP ETVNYVSAHDNETLFD + LKT  EISVD+RC VNHLATSI+ALSQG+PFFH+GDE+
Sbjct: 706  CPTETVNYVSAHDNETLFDVLSLKTPMEISVDERCSVNHLATSIIALSQGIPFFHAGDEM 765

Query: 1922 LRS 1930
            LRS
Sbjct: 766  LRS 768



 Score =  141 bits (356), Expect = 2e-31
 Identities = 66/103 (64%), Positives = 84/103 (81%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           +VK +LE    GLPENV++KFPHI+ YKAF VP S+D+ +L+KCQLAV SF  +GKC NA
Sbjct: 128 DVKVELEEDSGGLPENVIEKFPHIQGYKAFKVP-SLDIKNLLKCQLAVASFDSDGKCSNA 186

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 309
           TGLQLPG+LD++F+Y GPLGA+F EE++TL+LWAPTAQ    C
Sbjct: 187 TGLQLPGILDELFSYDGPLGALFLEESVTLFLWAPTAQAVRAC 229


>ref|XP_007220271.1| pullulanase 1, chloroplastic [Prunus persica]
 gb|ONI23747.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 965

 Score =  867 bits (2239), Expect = 0.0
 Identities = 405/539 (75%), Positives = 469/539 (87%)
 Frame = +2

Query: 314  VYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVNDPYA 493
            +YK+PSGG  L+ VQL+E +GVWS  GP+SWEGCYYVYEVSVYHPST +IE C  NDPYA
Sbjct: 233  IYKEPSGGSPLEVVQLEEFNGVWSTKGPKSWEGCYYVYEVSVYHPSTLRIEKCYANDPYA 292

Query: 494  RGISSDGMRTLLVNLDCEQLKPTGWDELAEEKPDLRSFSDISIYELHVRDFSASDETVNP 673
            RG+SSDG RTLLVNLD + +KP GWD+L +EKPD+ SFSDISIYELH+RDFSASD+ V+P
Sbjct: 293  RGLSSDGRRTLLVNLDSDNIKPEGWDKLVDEKPDILSFSDISIYELHIRDFSASDQAVHP 352

Query: 674  DFRGGFLAFTSKDSAGVLHLKKLSDAGLTHVHLLPCFQFGGVDDDRDKWKQLDFQELAKL 853
            +FRGG+LAFT +DSAG +HLKKLS+AG+THVHLLP FQF GVDD+++ WK +D + L K 
Sbjct: 353  EFRGGYLAFTLQDSAGAIHLKKLSNAGITHVHLLPAFQFAGVDDEKENWKSVDPEVLEKF 412

Query: 854  PSDSDKQQAIITSIHDQDGYNWGYNPVLWGVPKGSYASNPNGKSRIIDFRKMIQALNGIG 1033
            PSDSDKQQA+IT+I + DGYNWGYNPVLWGVPKGSYASN NG  R I+FRKM+QALN  G
Sbjct: 413  PSDSDKQQALITAIQNDDGYNWGYNPVLWGVPKGSYASNANGTYRAIEFRKMVQALNRFG 472

Query: 1034 LRVVLDVVYNHVHASGPLDTDSVLDKVVPGYYIRRNIDGFIENSTCINNTASEHLMVERL 1213
            LRVVLDVVYNH+H SGP+D +SVLDK+VPGYY+RRN DGFIE+STC+NNTASEH MVERL
Sbjct: 473  LRVVLDVVYNHLHGSGPVDDNSVLDKIVPGYYLRRNTDGFIEHSTCVNNTASEHFMVERL 532

Query: 1214 IIDDLLCWAVEYKVDGFRFDLMGHIMKSTMLKAKSALQSLSKDENGVDGSKIYIYGEGWD 1393
            I+DDLL WAV+YKVDGFRFDLMGHIM+ TM+KAK AL SL+K+ +GVDGS IYIYGEGWD
Sbjct: 533  IVDDLLHWAVDYKVDGFRFDLMGHIMRRTMVKAKDALCSLTKERDGVDGSSIYIYGEGWD 592

Query: 1394 FGEVAKNKRGINASQFNICGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQPNAYD 1573
            FGEVA N RGINASQFNI GTGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL LQPN +D
Sbjct: 593  FGEVANNGRGINASQFNIHGTGIGSFNDRIRDAILGGSPFGHPLQQGFVTGLLLQPNGHD 652

Query: 1574 YGSKAVTDDMLSASVDHIQVGLAANLGDFVLTGHRGEAVKGSEIWTHDGMPVGYALCPIE 1753
            +G +AV + ML+ S DHIQVG+AANL DFVLT   G+ VKGSE+ T+ G PV Y LCP E
Sbjct: 653  HGPEAVAEHMLAESKDHIQVGMAANLRDFVLTNCEGKEVKGSEVLTYGGTPVAYTLCPTE 712

Query: 1754 TVNYVSAHDNETLFDTICLKTAEEISVDDRCRVNHLATSIVALSQGVPFFHSGDEILRS 1930
            T+NYVSAHDNETLFD + LKT  EISV++RCR+NHLATSI+AL+QG+PFFHSGDEILRS
Sbjct: 713  TINYVSAHDNETLFDIVSLKTPMEISVEERCRINHLATSIIALAQGIPFFHSGDEILRS 771



 Score =  143 bits (361), Expect = 7e-32
 Identities = 63/103 (61%), Positives = 84/103 (81%)
 Frame = +1

Query: 1   EVKFQLEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVNGKCMNA 180
           +VK +LE    GLPENV +KFPHIKDY+AF+VP  +D   L+KCQLAV +F  +G+C +A
Sbjct: 130 DVKVKLEEDKHGLPENVKEKFPHIKDYRAFNVPPDLDAKPLLKCQLAVATFNSDGRCSDA 189

Query: 181 TGLQLPGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 309
           TGLQLPG+LDD+F+Y GPLGA++S+++++LYLWAPTAQ  C C
Sbjct: 190 TGLQLPGILDDLFSYNGPLGALYSKQSVSLYLWAPTAQEVCVC 232