BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon27_contig00018049
         (1252 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_008783492.1| PREDICTED: S-adenosyl-L-methionine-dependent...   761   0.0  
ref|XP_008783491.1| PREDICTED: S-adenosyl-L-methionine-dependent...   758   0.0  
ref|XP_010942945.1| PREDICTED: S-adenosyl-L-methionine-dependent...   758   0.0  
dbj|BAF13343.1| Os03g0775300 [Oryza sativa Japonica Group] >gi|9...   748   0.0  
ref|XP_015631211.1| PREDICTED: S-adenosyl-L-methionine-dependent...   748   0.0  
ref|NP_001347253.1| uncharacterized protein LOC111828519 [Oryza ...   733   0.0  
ref|XP_020704408.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   743   0.0  
ref|XP_009399072.1| PREDICTED: S-adenosyl-L-methionine-dependent...   742   0.0  
ref|NP_001347248.1| uncharacterized protein LOC100273754 [Zea ma...   741   0.0  
ref|XP_020092095.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   741   0.0  
gb|OAY80972.1| S-adenosyl-L-methionine-dependent tRNA 4-demethyl...   741   0.0  
ref|NP_001347259.1| uncharacterized LOC111828523 [Sorghum bicolor]    740   0.0  
gb|KQK86877.1| hypothetical protein SETIT_034607mg [Setaria ital...   739   0.0  
ref|NP_001347566.1| uncharacterized protein LOC111946219 [Setari...   739   0.0  
ref|XP_020180383.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   735   0.0  
dbj|BAK06688.1| predicted protein [Hordeum vulgare subsp. vulgare]    734   0.0  
gb|KZV28868.1| S-adenosyl-L-methionine-dependent tRNA 4-demethyl...   733   0.0  
ref|XP_014752193.1| PREDICTED: S-adenosyl-L-methionine-dependent...   731   0.0  
gb|OEL34423.1| S-adenosyl-L-methionine-dependent tRNA 4-demethyl...   729   0.0  
ref|XP_017981378.1| PREDICTED: S-adenosyl-L-methionine-dependent...   727   0.0  

>ref|XP_008783492.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like isoform X1 [Phoenix dactylifera]
          Length = 648

 Score =  761 bits (1964), Expect = 0.0
 Identities = 361/414 (87%), Positives = 386/414 (93%)
 Frame = +3

Query: 6    VDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGS 185
            VD+EDIAGKAPSRK   +L NG          NG+NG REMVTP+IR SL+KQGYKI+GS
Sbjct: 237  VDVEDIAGKAPSRKSTGALSNGGQ--------NGDNGLREMVTPIIRTSLEKQGYKIVGS 288

Query: 186  HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 365
            HSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS
Sbjct: 289  HSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 348

Query: 366  WRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEI 545
            WRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGV QE+L EGLSP+HCALSLVGEPIMYPEI
Sbjct: 349  WRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVKQEQLLEGLSPKHCALSLVGEPIMYPEI 408

Query: 546  NTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWE 725
            N+LIDELH RHISTFLVTNAQFPD+IK L+P+TQLYVSVDAATKDSLKAIDRPLF DFWE
Sbjct: 409  NSLIDELHSRHISTFLVTNAQFPDRIKMLRPVTQLYVSVDAATKDSLKAIDRPLFSDFWE 468

Query: 726  RFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATS 905
            RFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYA+L  IG PDLVE+KGVTYCGS+ATS
Sbjct: 469  RFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYANLLGIGKPDLVEMKGVTYCGSSATS 528

Query: 906  KLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDY 1085
            KLTMENVPWHSDVR FSEALAIKS+G+YEVACEHVHSCCV+LAKV K+K+ GQW+TWIDY
Sbjct: 529  KLTMENVPWHSDVRAFSEALAIKSSGEYEVACEHVHSCCVVLAKVSKFKVKGQWYTWIDY 588

Query: 1086 ERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            ERFHELVASGKPFKSE YMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAA+IR
Sbjct: 589  ERFHELVASGKPFKSEHYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAASIR 642


>ref|XP_008783491.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like isoform X2 [Phoenix dactylifera]
          Length = 648

 Score =  758 bits (1958), Expect = 0.0
 Identities = 360/414 (86%), Positives = 385/414 (92%)
 Frame = +3

Query: 6    VDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGS 185
            VD+EDIAGKAPSRK   +L NG          NG+NG REMVTP+I  SL+KQGYKI+GS
Sbjct: 237  VDVEDIAGKAPSRKSTGALSNGGQ--------NGDNGLREMVTPIITTSLEKQGYKIVGS 288

Query: 186  HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 365
            HSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS
Sbjct: 289  HSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 348

Query: 366  WRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEI 545
            WRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGV QE+L EGLSP+HCALSLVGEPIMYPEI
Sbjct: 349  WRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVKQEQLLEGLSPKHCALSLVGEPIMYPEI 408

Query: 546  NTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWE 725
            N+LIDELH RHISTFLVTNAQFPD+IK L+P+TQLYVSVDAATKDSLKAIDRPLF DFWE
Sbjct: 409  NSLIDELHSRHISTFLVTNAQFPDRIKMLRPVTQLYVSVDAATKDSLKAIDRPLFSDFWE 468

Query: 726  RFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATS 905
            RFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYA+L  IG PDLVE+KGVTYCGS+ATS
Sbjct: 469  RFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYANLLGIGKPDLVEMKGVTYCGSSATS 528

Query: 906  KLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDY 1085
            KLTMENVPWHSDVR FSEALAIKS+G+YEVACEHVHSCCV+LAKV K+K+ GQW+TWIDY
Sbjct: 529  KLTMENVPWHSDVRAFSEALAIKSSGEYEVACEHVHSCCVVLAKVSKFKVKGQWYTWIDY 588

Query: 1086 ERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            ERFHELVASGKPFKSE YMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAA+IR
Sbjct: 589  ERFHELVASGKPFKSEHYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAASIR 642


>ref|XP_010942945.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Elaeis guineensis]
          Length = 647

 Score =  758 bits (1957), Expect = 0.0
 Identities = 362/414 (87%), Positives = 383/414 (92%)
 Frame = +3

Query: 6    VDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGS 185
            VDMEDIAGKAPSRK   +L NG          NG+NG REMVTP+IR SL+KQGYKIIGS
Sbjct: 241  VDMEDIAGKAPSRKSTGALSNGGQ--------NGDNGLREMVTPIIRTSLEKQGYKIIGS 292

Query: 186  HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 365
            HSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS
Sbjct: 293  HSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 352

Query: 366  WRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEI 545
            WRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGV QE+L EGLSPRHCALSLVGEPIMYPEI
Sbjct: 353  WRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVKQEQLLEGLSPRHCALSLVGEPIMYPEI 412

Query: 546  NTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWE 725
            N+LIDELH RHISTFLVTNAQFPD+IK L+ +TQLYVSVDAATKDSLKAIDRPLF DFWE
Sbjct: 413  NSLIDELHSRHISTFLVTNAQFPDRIKMLRSVTQLYVSVDAATKDSLKAIDRPLFSDFWE 472

Query: 726  RFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATS 905
            RFVDSLKAL+DKEQRTVYRLTLVKGWNAEDIDAYA+L  IG PDLVEIKGVTYCGS+ATS
Sbjct: 473  RFVDSLKALQDKEQRTVYRLTLVKGWNAEDIDAYANLLGIGKPDLVEIKGVTYCGSSATS 532

Query: 906  KLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDY 1085
            KLTMENVPWHSDV+ FSEALA+KS+G+YEVACEHVHSCCVLLAK  K+K+ GQW TWIDY
Sbjct: 533  KLTMENVPWHSDVKAFSEALAVKSSGEYEVACEHVHSCCVLLAKASKFKVKGQWFTWIDY 592

Query: 1086 ERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            ERFHELVASGKPFKSEDYMA+TPSWAVYGAEEGGFDPDQSR KKERRHGAA+IR
Sbjct: 593  ERFHELVASGKPFKSEDYMALTPSWAVYGAEEGGFDPDQSRCKKERRHGAASIR 646


>dbj|BAF13343.1| Os03g0775300 [Oryza sativa Japonica Group]
 dbj|BAS86627.1| Os03g0775300 [Oryza sativa Japonica Group]
          Length = 600

 Score =  748 bits (1930), Expect = 0.0
 Identities = 352/416 (84%), Positives = 381/416 (91%), Gaps = 1/416 (0%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKII 179
            EVDMEDIAGKAP+ +         NG  E +  NG ENG R+MVTP+IR SL+KQGYKII
Sbjct: 191  EVDMEDIAGKAPAARR-------RNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKII 243

Query: 180  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 359
            GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG
Sbjct: 244  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 303

Query: 360  KSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYP 539
            KSW+WKMDDPL IVNAAIDQHTKM+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYP
Sbjct: 304  KSWKWKMDDPLDIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYP 363

Query: 540  EINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDF 719
            EIN LIDELHRRHISTFLVTNAQFPDKIK LKPITQLYVSVDAATK+SLKA+DRPLF DF
Sbjct: 364  EINVLIDELHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDF 423

Query: 720  WERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTA 899
            WERF+DSLK+L DK+QRTVYRLTLVKGWNAE+ID YA L  +G PD +EIKGVTYCGS+A
Sbjct: 424  WERFLDSLKSLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSA 483

Query: 900  TSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWI 1079
            TSKLTMENVPWHSDV+DFSEALA+KS G YEVACEH HSCCVLLAKVDK+KING+WHTWI
Sbjct: 484  TSKLTMENVPWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWI 543

Query: 1080 DYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            DY+RFHELV SGKPF+S+DYMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAAA++
Sbjct: 544  DYDRFHELVTSGKPFRSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAAALK 599


>ref|XP_015631211.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Oryza sativa Japonica Group]
 sp|Q8H8N3.1|TYW1_ORYSJ RecName: Full=S-adenosyl-L-methionine-dependent tRNA
            4-demethylwyosine synthase; AltName: Full=tRNA
            wybutosine-synthesizing protein 1 homolog
 gb|AAN65027.1| putative oxidoreductase [Oryza sativa Japonica Group]
 gb|ABF99122.1| radical S-adenosyl methionine and flavodoxin domains 1, isoform 1,
            putative, expressed [Oryza sativa Japonica Group]
 gb|EAY92018.1| hypothetical protein OsI_13711 [Oryza sativa Indica Group]
          Length = 653

 Score =  748 bits (1930), Expect = 0.0
 Identities = 352/416 (84%), Positives = 381/416 (91%), Gaps = 1/416 (0%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKII 179
            EVDMEDIAGKAP+ +         NG  E +  NG ENG R+MVTP+IR SL+KQGYKII
Sbjct: 244  EVDMEDIAGKAPAARR-------RNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKII 296

Query: 180  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 359
            GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG
Sbjct: 297  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 356

Query: 360  KSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYP 539
            KSW+WKMDDPL IVNAAIDQHTKM+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYP
Sbjct: 357  KSWKWKMDDPLDIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYP 416

Query: 540  EINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDF 719
            EIN LIDELHRRHISTFLVTNAQFPDKIK LKPITQLYVSVDAATK+SLKA+DRPLF DF
Sbjct: 417  EINVLIDELHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDF 476

Query: 720  WERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTA 899
            WERF+DSLK+L DK+QRTVYRLTLVKGWNAE+ID YA L  +G PD +EIKGVTYCGS+A
Sbjct: 477  WERFLDSLKSLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSA 536

Query: 900  TSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWI 1079
            TSKLTMENVPWHSDV+DFSEALA+KS G YEVACEH HSCCVLLAKVDK+KING+WHTWI
Sbjct: 537  TSKLTMENVPWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWI 596

Query: 1080 DYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            DY+RFHELV SGKPF+S+DYMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAAA++
Sbjct: 597  DYDRFHELVTSGKPFRSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAAALK 652


>ref|NP_001347253.1| uncharacterized protein LOC111828519 [Oryza brachyantha]
          Length = 406

 Score =  733 bits (1892), Expect = 0.0
 Identities = 344/411 (83%), Positives = 376/411 (91%), Gaps = 1/411 (0%)
 Frame = +3

Query: 12   MEDIAGKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSH 188
            MEDIAGKA +R+        +NG  E +  NG ENG R+MVTP+IRASL+KQGYKIIGSH
Sbjct: 1    MEDIAGKAQARR--------QNGKVEGALSNGGENGPRDMVTPIIRASLEKQGYKIIGSH 52

Query: 189  SGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW 368
            SGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW
Sbjct: 53   SGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW 112

Query: 369  RWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEIN 548
            +WKMDDPL IVN AIDQHTKM+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYPEIN
Sbjct: 113  KWKMDDPLDIVNTAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEIN 172

Query: 549  TLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWER 728
             L+ ELHRRHISTFLVTNAQFPDKIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWER
Sbjct: 173  VLVGELHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWER 232

Query: 729  FVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSK 908
            F+DSLK+L +K+QRTVYRLTLVKGWN E+IDAYA L ++G PD +EIKGVTYCGS+ TSK
Sbjct: 233  FLDSLKSLHEKDQRTVYRLTLVKGWNVEEIDAYAKLLNLGQPDFIEIKGVTYCGSSTTSK 292

Query: 909  LTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYE 1088
            LTMENVPWHSDV++FSEALA+KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+
Sbjct: 293  LTMENVPWHSDVKEFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYD 352

Query: 1089 RFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAA 1241
            RFH+LV SGKPFKS+DYMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAAA
Sbjct: 353  RFHDLVTSGKPFKSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAAA 403


>ref|XP_020704408.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Dendrobium catenatum]
          Length = 664

 Score =  743 bits (1918), Expect = 0.0
 Identities = 352/414 (85%), Positives = 381/414 (92%)
 Frame = +3

Query: 6    VDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGS 185
            VD+EDIAGKAPSRK+ A LVNG NGV            +EMVTP+IR +L+KQGYKIIGS
Sbjct: 245  VDLEDIAGKAPSRKMKAELVNG-NGV-----------IKEMVTPIIRNNLEKQGYKIIGS 292

Query: 186  HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 365
            HSGVKICRWTK+QLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 
Sbjct: 293  HSGVKICRWTKAQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKE 352

Query: 366  WRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEI 545
            WRWKMDDPLVIV+AAIDQHTKMIKQMKGVPGV +++  EGLSPRHCALSLVGEPIMYPEI
Sbjct: 353  WRWKMDDPLVIVDAAIDQHTKMIKQMKGVPGVKEQQFLEGLSPRHCALSLVGEPIMYPEI 412

Query: 546  NTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWE 725
            N LI+ELHRRHISTFLVTNAQFPD+IK LKPITQLYVSVDAATK+SLKA+DRPLFGDFWE
Sbjct: 413  NRLIEELHRRHISTFLVTNAQFPDRIKMLKPITQLYVSVDAATKESLKAVDRPLFGDFWE 472

Query: 726  RFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATS 905
            RFVDSLKAL DK+QRTVYRLTLVKGWNAE++DAYA+L  +G PD +EIKGVTYCGSTATS
Sbjct: 473  RFVDSLKALHDKQQRTVYRLTLVKGWNAEEVDAYANLLGLGEPDFIEIKGVTYCGSTATS 532

Query: 906  KLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDY 1085
            KLTMENVPWHSDV++FSE LAIKSNG YEVACEHVHSCCVLLAKVDK+KINGQWHTWIDY
Sbjct: 533  KLTMENVPWHSDVKEFSETLAIKSNGLYEVACEHVHSCCVLLAKVDKFKINGQWHTWIDY 592

Query: 1086 ERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            ERFH+LVASGKPF +EDYMAVTPSWAVYGAEEGGFDP Q R+KKERRHG AA++
Sbjct: 593  ERFHDLVASGKPFGTEDYMAVTPSWAVYGAEEGGFDPKQFRYKKERRHGPAALK 646


>ref|XP_009399072.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Musa acuminata subsp. malaccensis]
          Length = 647

 Score =  742 bits (1915), Expect = 0.0
 Identities = 350/414 (84%), Positives = 383/414 (92%)
 Frame = +3

Query: 6    VDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGS 185
            VDMEDIAGKAPSR+   +L NG            ENG ++MVTPVIR +L+KQGYKIIGS
Sbjct: 244  VDMEDIAGKAPSRRPSVALTNG-----------WENGVKDMVTPVIRVNLEKQGYKIIGS 292

Query: 186  HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 365
            HSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS
Sbjct: 293  HSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 352

Query: 366  WRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEI 545
            WRWKMD+PL IV+ AID+HTKMIKQMKGVPGV  ERLSEGLSPRHCALSLVGEPIMYPEI
Sbjct: 353  WRWKMDNPLEIVDVAIDEHTKMIKQMKGVPGVKPERLSEGLSPRHCALSLVGEPIMYPEI 412

Query: 546  NTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWE 725
            NTL+DELHRRHISTFLVTNAQFPD+IK LKPITQLYVSVDAATKDSLKAIDRPLFGDFWE
Sbjct: 413  NTLVDELHRRHISTFLVTNAQFPDRIKMLKPITQLYVSVDAATKDSLKAIDRPLFGDFWE 472

Query: 726  RFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATS 905
            RF+DSLKAL+DKEQRTVYRLTLVKGWNAED++AYA+L  IG PDLVEIKGVTYCG++ATS
Sbjct: 473  RFLDSLKALQDKEQRTVYRLTLVKGWNAEDVEAYANLLGIGKPDLVEIKGVTYCGTSATS 532

Query: 906  KLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDY 1085
            KLTM+NVPWHSDV+ FSEALA+KS+G+YEVACEH HSCCVLLAKVDK+KI G+W+TWIDY
Sbjct: 533  KLTMDNVPWHSDVKAFSEALAMKSSGEYEVACEHAHSCCVLLAKVDKFKIKGEWYTWIDY 592

Query: 1086 ERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            ERFHELVASGK FK+EDYMA TPSWAVYGAEEGGFDPDQSR++KERRHGAA+++
Sbjct: 593  ERFHELVASGKSFKTEDYMARTPSWAVYGAEEGGFDPDQSRYRKERRHGAASVQ 646


>ref|NP_001347248.1| uncharacterized protein LOC100273754 [Zea mays]
 gb|ONM09874.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Zea mays]
          Length = 642

 Score =  741 bits (1912), Expect = 0.0
 Identities = 348/417 (83%), Positives = 378/417 (90%), Gaps = 1/417 (0%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKII 179
            EVDMEDIAGKAP+R+        +NG  E    NG  NG ++MVTP+IR SL+KQGYKII
Sbjct: 234  EVDMEDIAGKAPARR--------KNGNLEGVLANGVHNGVKDMVTPIIRTSLEKQGYKII 285

Query: 180  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 359
            GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG
Sbjct: 286  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 345

Query: 360  KSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYP 539
            KSW+WKMDDPL IVNAAIDQHTKM+KQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYP
Sbjct: 346  KSWKWKMDDPLDIVNAAIDQHTKMVKQMKGVPGVKPEKLDEGLSPRHCALSLVGEPIMYP 405

Query: 540  EINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDF 719
            EIN L+DELHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DF
Sbjct: 406  EINALVDELHRRHISTFLVTNAQFPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDF 465

Query: 720  WERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTA 899
            WERF+DSLK+L +K+QRTVYRLTLVKGWNAE+IDAYA L D+G PD +EIKGVTYCGS+A
Sbjct: 466  WERFLDSLKSLHEKDQRTVYRLTLVKGWNAEEIDAYAKLLDLGQPDFIEIKGVTYCGSSA 525

Query: 900  TSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWI 1079
            TSKLTMENVPWHSDV++FSE LA KS G YEVACEH HSCCVLLAKVDK+KING+WHTWI
Sbjct: 526  TSKLTMENVPWHSDVKEFSEVLASKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWI 585

Query: 1080 DYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIRA 1250
            DY+RFHELV SGKPF S DYMA+TPSWAVYGAEEGGFDPDQSRFKKERRHG AA+R+
Sbjct: 586  DYDRFHELVTSGKPFNSRDYMAMTPSWAVYGAEEGGFDPDQSRFKKERRHGTAALRS 642


>ref|XP_020092095.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Ananas comosus]
          Length = 655

 Score =  741 bits (1913), Expect = 0.0
 Identities = 353/412 (85%), Positives = 384/412 (93%), Gaps = 1/412 (0%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNGE-NGAREMVTPVIRASLQKQGYKII 179
            EVDMEDIAGKAPSRK   +LVNG++     + VNG+ +G REMVTP+IR SL+KQGYKII
Sbjct: 245  EVDMEDIAGKAPSRKSSVTLVNGKSS---GTLVNGKGDGLREMVTPIIRTSLEKQGYKII 301

Query: 180  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 359
            GSHSGVK+CRWTKSQLRGRGGCYKH+FYGIESHRCMEATPSLACANKCVFCWRHH NPVG
Sbjct: 302  GSHSGVKLCRWTKSQLRGRGGCYKHAFYGIESHRCMEATPSLACANKCVFCWRHHANPVG 361

Query: 360  KSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYP 539
            KSWRWKMDDPL IV+AAIDQHTKMIKQMKGVPGV QERL EGLSPRHCALSLVGEPIMYP
Sbjct: 362  KSWRWKMDDPLEIVDAAIDQHTKMIKQMKGVPGVKQERLDEGLSPRHCALSLVGEPIMYP 421

Query: 540  EINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDF 719
            EINTLIDELH+R ISTFLVTNAQFPD+IK LKPITQLYVSVDA+TK+SLKAIDRPLFGDF
Sbjct: 422  EINTLIDELHQRRISTFLVTNAQFPDRIKMLKPITQLYVSVDASTKESLKAIDRPLFGDF 481

Query: 720  WERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTA 899
            WERF+DSLKAL DKEQRTVYRLTLVKGWNAE+IDAYA+L  +G PDLVEIKGVTYCGS+A
Sbjct: 482  WERFLDSLKALHDKEQRTVYRLTLVKGWNAEEIDAYANLLTMGKPDLVEIKGVTYCGSSA 541

Query: 900  TSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWI 1079
            TSKLTMENVPWHSDV+ FSEALA+KSNG YEVACEHVHSCCVLLAKVDK+K++G+W+TWI
Sbjct: 542  TSKLTMENVPWHSDVKAFSEALAVKSNGIYEVACEHVHSCCVLLAKVDKFKVDGKWYTWI 601

Query: 1080 DYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGA 1235
            DYERF ELVASG PF++EDY A+TPSWAVYGAEEGGFDP QSR+KKERRHGA
Sbjct: 602  DYERFQELVASGTPFRTEDYTALTPSWAVYGAEEGGFDPGQSRYKKERRHGA 653


>gb|OAY80972.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Ananas comosus]
          Length = 663

 Score =  741 bits (1913), Expect = 0.0
 Identities = 353/412 (85%), Positives = 384/412 (93%), Gaps = 1/412 (0%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNGE-NGAREMVTPVIRASLQKQGYKII 179
            EVDMEDIAGKAPSRK   +LVNG++     + VNG+ +G REMVTP+IR SL+KQGYKII
Sbjct: 253  EVDMEDIAGKAPSRKSSVTLVNGKSS---GTLVNGKGDGLREMVTPIIRTSLEKQGYKII 309

Query: 180  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 359
            GSHSGVK+CRWTKSQLRGRGGCYKH+FYGIESHRCMEATPSLACANKCVFCWRHH NPVG
Sbjct: 310  GSHSGVKLCRWTKSQLRGRGGCYKHAFYGIESHRCMEATPSLACANKCVFCWRHHANPVG 369

Query: 360  KSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYP 539
            KSWRWKMDDPL IV+AAIDQHTKMIKQMKGVPGV QERL EGLSPRHCALSLVGEPIMYP
Sbjct: 370  KSWRWKMDDPLEIVDAAIDQHTKMIKQMKGVPGVKQERLDEGLSPRHCALSLVGEPIMYP 429

Query: 540  EINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDF 719
            EINTLIDELH+R ISTFLVTNAQFPD+IK LKPITQLYVSVDA+TK+SLKAIDRPLFGDF
Sbjct: 430  EINTLIDELHQRRISTFLVTNAQFPDRIKMLKPITQLYVSVDASTKESLKAIDRPLFGDF 489

Query: 720  WERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTA 899
            WERF+DSLKAL DKEQRTVYRLTLVKGWNAE+IDAYA+L  +G PDLVEIKGVTYCGS+A
Sbjct: 490  WERFLDSLKALHDKEQRTVYRLTLVKGWNAEEIDAYANLLTMGKPDLVEIKGVTYCGSSA 549

Query: 900  TSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWI 1079
            TSKLTMENVPWHSDV+ FSEALA+KSNG YEVACEHVHSCCVLLAKVDK+K++G+W+TWI
Sbjct: 550  TSKLTMENVPWHSDVKAFSEALAVKSNGIYEVACEHVHSCCVLLAKVDKFKVDGKWYTWI 609

Query: 1080 DYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGA 1235
            DYERF ELVASG PF++EDY A+TPSWAVYGAEEGGFDP QSR+KKERRHGA
Sbjct: 610  DYERFQELVASGTPFRTEDYTALTPSWAVYGAEEGGFDPGQSRYKKERRHGA 661


>ref|NP_001347259.1| uncharacterized LOC111828523 [Sorghum bicolor]
          Length = 652

 Score =  740 bits (1910), Expect = 0.0
 Identities = 348/414 (84%), Positives = 377/414 (91%), Gaps = 1/414 (0%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKII 179
            EVDMEDIAGKAP+R+        +NG  E    NG +NG ++MVTP+IR SL+KQGYKII
Sbjct: 242  EVDMEDIAGKAPARR--------KNGNLEGVVANGVQNGVKDMVTPIIRTSLEKQGYKII 293

Query: 180  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 359
            GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG
Sbjct: 294  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 353

Query: 360  KSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYP 539
            KSW+WKMDDPL IVNAAIDQHTKM+KQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYP
Sbjct: 354  KSWKWKMDDPLDIVNAAIDQHTKMVKQMKGVPGVKPEKLEEGLSPRHCALSLVGEPIMYP 413

Query: 540  EINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDF 719
            EIN L+DELHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DF
Sbjct: 414  EINALVDELHRRHISTFLVTNAQFPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDF 473

Query: 720  WERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTA 899
            WERF+DSLK+L +K+QRTVYRLTLVKGWNAE+IDAYA L D+G PD +EIKGVTYCGS+A
Sbjct: 474  WERFLDSLKSLHEKDQRTVYRLTLVKGWNAEEIDAYAKLLDLGQPDFIEIKGVTYCGSSA 533

Query: 900  TSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWI 1079
            TSKLTMENVPWHSDV++FSE LA KS G YEVACEH HSCCVLLAKVDK+KING+WHTWI
Sbjct: 534  TSKLTMENVPWHSDVKEFSEVLASKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWI 593

Query: 1080 DYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAA 1241
            DY+RFHELV SGKPFKS DYMA+TPSWAVYGAEEGGFDPDQSRFKKERRHG AA
Sbjct: 594  DYDRFHELVTSGKPFKSRDYMAMTPSWAVYGAEEGGFDPDQSRFKKERRHGTAA 647


>gb|KQK86877.1| hypothetical protein SETIT_034607mg [Setaria italica]
          Length = 649

 Score =  739 bits (1909), Expect = 0.0
 Identities = 346/416 (83%), Positives = 379/416 (91%), Gaps = 1/416 (0%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKII 179
            E+DMEDIAGKAP+R         +NG  E S  NG +NG +EMVTP+IR SL+KQGYKI+
Sbjct: 242  EIDMEDIAGKAPAR---------QNGKVEGSLANGGQNGVKEMVTPIIRTSLEKQGYKIL 292

Query: 180  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 359
            GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG
Sbjct: 293  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 352

Query: 360  KSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYP 539
            KSW+WKMD+PL I NAAIDQHTKMIKQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYP
Sbjct: 353  KSWKWKMDNPLDIANAAIDQHTKMIKQMKGVPGVKPEKLEEGLSPRHCALSLVGEPIMYP 412

Query: 540  EINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDF 719
            EIN L+DELHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DF
Sbjct: 413  EINALVDELHRRHISTFLVTNAQFPEKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDF 472

Query: 720  WERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTA 899
            WERF+DSLK+L +K+QRTVYRLTLVKGWNAE+IDAYA L ++G PD +EIKGVTYCGS+A
Sbjct: 473  WERFLDSLKSLHEKDQRTVYRLTLVKGWNAEEIDAYAKLLNLGQPDFIEIKGVTYCGSSA 532

Query: 900  TSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWI 1079
            TSKLTMENVPWHSDV++FSE LA KS G YEVACEH HSCCVLLAKVDK+KING+WHTWI
Sbjct: 533  TSKLTMENVPWHSDVKEFSEVLASKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWI 592

Query: 1080 DYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            DY+RFHELV SGKPFKS+DYMA+TPSWAVYGAEEGGFDPDQSRFKKERRHG AA++
Sbjct: 593  DYDRFHELVTSGKPFKSQDYMALTPSWAVYGAEEGGFDPDQSRFKKERRHGTAALK 648


>ref|NP_001347566.1| uncharacterized protein LOC111946219 [Setaria italica]
          Length = 692

 Score =  739 bits (1909), Expect = 0.0
 Identities = 346/416 (83%), Positives = 379/416 (91%), Gaps = 1/416 (0%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKII 179
            E+DMEDIAGKAP+R         +NG  E S  NG +NG +EMVTP+IR SL+KQGYKI+
Sbjct: 285  EIDMEDIAGKAPAR---------QNGKVEGSLANGGQNGVKEMVTPIIRTSLEKQGYKIL 335

Query: 180  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 359
            GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG
Sbjct: 336  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 395

Query: 360  KSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYP 539
            KSW+WKMD+PL I NAAIDQHTKMIKQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYP
Sbjct: 396  KSWKWKMDNPLDIANAAIDQHTKMIKQMKGVPGVKPEKLEEGLSPRHCALSLVGEPIMYP 455

Query: 540  EINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDF 719
            EIN L+DELHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DF
Sbjct: 456  EINALVDELHRRHISTFLVTNAQFPEKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDF 515

Query: 720  WERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTA 899
            WERF+DSLK+L +K+QRTVYRLTLVKGWNAE+IDAYA L ++G PD +EIKGVTYCGS+A
Sbjct: 516  WERFLDSLKSLHEKDQRTVYRLTLVKGWNAEEIDAYAKLLNLGQPDFIEIKGVTYCGSSA 575

Query: 900  TSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWI 1079
            TSKLTMENVPWHSDV++FSE LA KS G YEVACEH HSCCVLLAKVDK+KING+WHTWI
Sbjct: 576  TSKLTMENVPWHSDVKEFSEVLASKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWI 635

Query: 1080 DYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            DY+RFHELV SGKPFKS+DYMA+TPSWAVYGAEEGGFDPDQSRFKKERRHG AA++
Sbjct: 636  DYDRFHELVTSGKPFKSQDYMALTPSWAVYGAEEGGFDPDQSRFKKERRHGTAALK 691


>ref|XP_020180383.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Aegilops tauschii subsp. tauschii]
          Length = 659

 Score =  735 bits (1898), Expect = 0.0
 Identities = 344/416 (82%), Positives = 381/416 (91%), Gaps = 1/416 (0%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKII 179
            EVDMEDIAGKA ++K        +NG  E +  NG +NGAR MVTP+IRASL+KQGYKI+
Sbjct: 251  EVDMEDIAGKATAKK--------QNGKVESALPNGGQNGARAMVTPIIRASLEKQGYKIV 302

Query: 180  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 359
            GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG
Sbjct: 303  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 362

Query: 360  KSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYP 539
            KSW+WKMDDPL IVN AID+H KM+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYP
Sbjct: 363  KSWKWKMDDPLDIVNTAIDEHRKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYP 422

Query: 540  EINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDF 719
            EINTL+D+LHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DF
Sbjct: 423  EINTLVDDLHRRHISTFLVTNAQFPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDF 482

Query: 720  WERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTA 899
            WERF+DSLK+L DK+QRTVYRLTLVKGWN E+IDAYA+L  +G PD +EIKGVTYCGS+A
Sbjct: 483  WERFLDSLKSLHDKDQRTVYRLTLVKGWNVEEIDAYANLLKLGQPDFIEIKGVTYCGSSA 542

Query: 900  TSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWI 1079
            TSKLTMENVPWH+DV++FSE LA KS G YE+ACEHVHSCCVLLAKVDK+KING+WHTWI
Sbjct: 543  TSKLTMENVPWHADVKEFSEVLASKSGGVYELACEHVHSCCVLLAKVDKFKINGKWHTWI 602

Query: 1080 DYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            DY+RF+ELV SGKPFKS DYMAVTPSWAVYGA+EGGFDPDQ+RFKKERRHGAAA++
Sbjct: 603  DYDRFNELVTSGKPFKSSDYMAVTPSWAVYGADEGGFDPDQARFKKERRHGAAALK 658


>dbj|BAK06688.1| predicted protein [Hordeum vulgare subsp. vulgare]
          Length = 664

 Score =  734 bits (1895), Expect = 0.0
 Identities = 343/416 (82%), Positives = 381/416 (91%), Gaps = 1/416 (0%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKII 179
            EVDMEDIAGKA ++K        +NG  E +  NG +NGAR MVTP+IRASL+KQGYKI+
Sbjct: 256  EVDMEDIAGKATAKK--------QNGKVEGALPNGGQNGARAMVTPIIRASLEKQGYKIV 307

Query: 180  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 359
            GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG
Sbjct: 308  GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVG 367

Query: 360  KSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYP 539
            KSW+WKMDDPL IVN AID+H KM+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYP
Sbjct: 368  KSWKWKMDDPLDIVNTAIDEHRKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYP 427

Query: 540  EINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDF 719
            EINTL+D+LHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DF
Sbjct: 428  EINTLVDDLHRRHISTFLVTNAQFPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDF 487

Query: 720  WERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTA 899
            WERF+DSLK+L DK+QRTVYRLTLVKGWN E+IDAYA+L  +G PD +E+KGVTYCGS+A
Sbjct: 488  WERFLDSLKSLHDKDQRTVYRLTLVKGWNVEEIDAYANLLKLGQPDFIEVKGVTYCGSSA 547

Query: 900  TSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWI 1079
            TSKLTMENVPWH+DV++FSE LA KS G YE+ACEHVHSCCVLLAKVDK+KING+WHTWI
Sbjct: 548  TSKLTMENVPWHADVKEFSEVLATKSGGVYELACEHVHSCCVLLAKVDKFKINGKWHTWI 607

Query: 1080 DYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            DY+RF+ELV SGKPFKS DYMAVTPSWAVYGA+EGGFDPDQ+RFKKERRHGAAA++
Sbjct: 608  DYDRFNELVMSGKPFKSSDYMAVTPSWAVYGADEGGFDPDQARFKKERRHGAAALK 663


>gb|KZV28868.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Dorcoceras hygrometricum]
          Length = 658

 Score =  733 bits (1891), Expect = 0.0
 Identities = 350/417 (83%), Positives = 379/417 (90%), Gaps = 3/417 (0%)
 Frame = +3

Query: 6    VDMEDIAGKAPSRK-LVASLVNGENGVKEKSFVNGE--NGAREMVTPVIRASLQKQGYKI 176
            VD+EDIAGK PSRK ++ +  NG+        VNGE  NG +EMVTPVIRASLQKQGYKI
Sbjct: 247  VDLEDIAGKGPSRKSIMGTTANGK--------VNGEVVNGEKEMVTPVIRASLQKQGYKI 298

Query: 177  IGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPV 356
            IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPV
Sbjct: 299  IGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPV 358

Query: 357  GKSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMY 536
            GKSWRWKMDDPLVIV+ AID HTKMI+QMKGVPGV  ERLSEGLSPRHCALSLVGEPIMY
Sbjct: 359  GKSWRWKMDDPLVIVDTAIDLHTKMIRQMKGVPGVKAERLSEGLSPRHCALSLVGEPIMY 418

Query: 537  PEINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGD 716
            PEIN LIDELHRR ISTFLVTNAQFP++IK LKPITQLYVSVDAATKDSLKAIDRPLFGD
Sbjct: 419  PEINALIDELHRRRISTFLVTNAQFPERIKTLKPITQLYVSVDAATKDSLKAIDRPLFGD 478

Query: 717  FWERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGST 896
            FWERFV+SLKALR+K+QRTVYRLTLVKGWN EDIDAY+SLFD+GNPD +EIKGVTYCGS+
Sbjct: 479  FWERFVESLKALREKQQRTVYRLTLVKGWNTEDIDAYSSLFDLGNPDFIEIKGVTYCGSS 538

Query: 897  ATSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTW 1076
            ATSKLTMENVPWHSDV++FSEALA KSNG YEVACEH HSCCVLL KV K+K+ G+W TW
Sbjct: 539  ATSKLTMENVPWHSDVKNFSEALAQKSNGAYEVACEHEHSCCVLLGKVSKFKVEGEWFTW 598

Query: 1077 IDYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            IDY++FH+LVASGKPF SEDYM+ TP WAVYGAEEGGFDPDQSRFKKERRH  +A +
Sbjct: 599  IDYDKFHDLVASGKPFTSEDYMSPTPRWAVYGAEEGGFDPDQSRFKKERRHHKSAAK 655


>ref|XP_014752193.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Brachypodium distachyon]
          Length = 653

 Score =  731 bits (1888), Expect = 0.0
 Identities = 341/415 (82%), Positives = 377/415 (90%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIG 182
            EVDMED+AGKA +R+    +    NG         ENGAREMVTP+IR SL+KQGYKI+G
Sbjct: 246  EVDMEDLAGKATARRQNGKVAGLPNGA--------ENGAREMVTPIIRTSLEKQGYKIVG 297

Query: 183  SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 362
            SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK
Sbjct: 298  SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 357

Query: 363  SWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPE 542
            SW+WKMDDPL IVN AID+H KM+KQMKGVPGV  E L+EGLSPRHCALSLVGEPIMYPE
Sbjct: 358  SWKWKMDDPLDIVNTAIDEHRKMVKQMKGVPGVKPELLAEGLSPRHCALSLVGEPIMYPE 417

Query: 543  INTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFW 722
            INTL+D+LHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFW
Sbjct: 418  INTLVDDLHRRHISTFLVTNAQFPEKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFW 477

Query: 723  ERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTAT 902
            ERF+DSLK+L DK+QRTVYRLTLVKGWNAE+IDAYA+L ++G PD +EIKGVTYCGS+AT
Sbjct: 478  ERFLDSLKSLHDKDQRTVYRLTLVKGWNAEEIDAYANLLNLGQPDFIEIKGVTYCGSSAT 537

Query: 903  SKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWID 1082
            SKLTMENVPWH+DV++FSE LA KS G YE+ACEHVHSCCVLLAKVDK+KING+WHTWID
Sbjct: 538  SKLTMENVPWHADVKEFSEVLASKSGGVYELACEHVHSCCVLLAKVDKFKINGKWHTWID 597

Query: 1083 YERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 1247
            Y+RFHELV SGKPFKS DYMA+TPSWAVYGAEEGGFDPDQSR+KKERRHG AA++
Sbjct: 598  YDRFHELVTSGKPFKSCDYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGTAALK 652


>gb|OEL34423.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Dichanthelium oligosanthes]
          Length = 674

 Score =  729 bits (1881), Expect = 0.0
 Identities = 347/438 (79%), Positives = 378/438 (86%), Gaps = 23/438 (5%)
 Frame = +3

Query: 3    EVDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQ----- 164
            E+DMEDIAGKAP+R+        +NG  E    NG ENG +EMVTP+IR SL+KQ     
Sbjct: 244  EIDMEDIAGKAPARR--------QNGKVEGGLANGGENGVKEMVTPIIRTSLEKQLGSVL 295

Query: 165  -----------------GYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEA 293
                             GYKI+GSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEA
Sbjct: 296  YFYYIYSIPIHILCVLQGYKILGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEA 355

Query: 294  TPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQER 473
            TPSLACANKCVFCWRHHTNPVGKSW+WKMDDPL IVNAAIDQHTKM+KQMKGVPGV  E+
Sbjct: 356  TPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNAAIDQHTKMVKQMKGVPGVKPEK 415

Query: 474  LSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLY 653
            L EGLSPRHCALSLVGEPIMYPEIN L+DELHRRHISTFLVTNAQFP+KIK LKPITQLY
Sbjct: 416  LEEGLSPRHCALSLVGEPIMYPEINALVDELHRRHISTFLVTNAQFPEKIKTLKPITQLY 475

Query: 654  VSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYAS 833
            VSVDAATK+SLKA+DRPLF DFWERF+DSLK+L +K+QRTVYRLTLVKGWNAE+IDAYA 
Sbjct: 476  VSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHEKDQRTVYRLTLVKGWNAEEIDAYAK 535

Query: 834  LFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVH 1013
            L D+G PD +EIKGVTYCGS+ATSKLTMENVPWHSDV++FSE LA KS G YEVACEH H
Sbjct: 536  LLDLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKEFSEVLASKSGGVYEVACEHAH 595

Query: 1014 SCCVLLAKVDKYKINGQWHTWIDYERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFD 1193
            SCCVLLAKVDK+KING+WHTWIDY+RFHELV SGKPFKS+ YMA TPSWAVYGAEEGGFD
Sbjct: 596  SCCVLLAKVDKFKINGKWHTWIDYDRFHELVTSGKPFKSQHYMAETPSWAVYGAEEGGFD 655

Query: 1194 PDQSRFKKERRHGAAAIR 1247
            PDQSRFKKERRHG AA++
Sbjct: 656  PDQSRFKKERRHGTAALK 673


>ref|XP_017981378.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Theobroma cacao]
          Length = 637

 Score =  727 bits (1876), Expect = 0.0
 Identities = 344/409 (84%), Positives = 371/409 (90%), Gaps = 1/409 (0%)
 Frame = +3

Query: 6    VDMEDIAGKAPSRKLVASLVNGENGVKEKSFVNGE-NGAREMVTPVIRASLQKQGYKIIG 182
            VD+EDIAGK PSRK   ++             NG+ +G REMVTPVIRA+L+KQGYKIIG
Sbjct: 235  VDLEDIAGKGPSRKKSVNVAE----------TNGKMDGKREMVTPVIRANLEKQGYKIIG 284

Query: 183  SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 362
            SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK
Sbjct: 285  SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 344

Query: 363  SWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPE 542
            SW+WKMDDPL IVN AID HTKMIKQMKGVPGVTQERL+EGL+PRHCALSLVGEPIMYPE
Sbjct: 345  SWQWKMDDPLEIVNTAIDLHTKMIKQMKGVPGVTQERLTEGLTPRHCALSLVGEPIMYPE 404

Query: 543  INTLIDELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFW 722
            IN L+DELHRR ISTFLVTNAQFPDKIK LKPITQLYVSVDAATKDSLKAIDRPLFGDFW
Sbjct: 405  INMLVDELHRRRISTFLVTNAQFPDKIKMLKPITQLYVSVDAATKDSLKAIDRPLFGDFW 464

Query: 723  ERFVDSLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTAT 902
            ERF+DSLKALRDK+QRTVYRLTLVKGWN ED DAY+ LF IG PD VEIKGVTYCGS+AT
Sbjct: 465  ERFIDSLKALRDKQQRTVYRLTLVKGWNTEDADAYSKLFSIGKPDFVEIKGVTYCGSSAT 524

Query: 903  SKLTMENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWID 1082
            SKLTMENVPWHSDV+ FSEALA+KS G+YEVACEH HSCCVLLAK DK+K+NGQW+TWID
Sbjct: 525  SKLTMENVPWHSDVKAFSEALALKSEGEYEVACEHAHSCCVLLAKTDKFKVNGQWYTWID 584

Query: 1083 YERFHELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRH 1229
            YE+FH+LVASG+PF S DYMA+TPSWAVYGAEEGGFDPDQSR++KER H
Sbjct: 585  YEKFHDLVASGEPFDSADYMALTPSWAVYGAEEGGFDPDQSRYRKERHH 633