BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon27_contig00015177
         (1297 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ONK57653.1| uncharacterized protein A4U43_C09F2700 [Asparagus...   730   0.0  
ref|XP_008786520.1| PREDICTED: probable thiamine biosynthetic bi...   701   0.0  
ref|XP_008786519.1| PREDICTED: probable thiamine biosynthetic bi...   701   0.0  
ref|XP_010930605.2| PREDICTED: probable thiamine biosynthetic bi...   701   0.0  
ref|XP_020246005.1| probable thiamine biosynthetic bifunctional ...   688   0.0  
ref|XP_017697680.1| PREDICTED: probable thiamine biosynthetic bi...   692   0.0  
gb|OAY79547.1| putative thiamine biosynthetic bifunctional enzym...   681   0.0  
ref|XP_020106624.1| probable thiamine biosynthetic bifunctional ...   681   0.0  
ref|NP_001105900.2| phosphomethylpyrimidine kinase/thiamin-phosp...   677   0.0  
gb|PAN16326.1| hypothetical protein PAHAL_C00668 [Panicum hallii]     668   0.0  
ref|XP_004977163.1| probable thiamine biosynthetic bifunctional ...   667   0.0  
ref|XP_006664409.2| PREDICTED: probable thiamine biosynthetic bi...   666   0.0  
ref|XP_002442970.1| probable thiamine biosynthetic bifunctional ...   664   0.0  
emb|CAJ45026.1| phosphomethylpyrimidine kinase/thiamin-phosphate...   664   0.0  
gb|PIA56493.1| hypothetical protein AQUCO_00700673v1 [Aquilegia ...   661   0.0  
ref|XP_009404307.1| PREDICTED: probable thiamine biosynthetic bi...   661   0.0  
emb|CBI29762.3| unnamed protein product, partial [Vitis vinifera]     657   0.0  
ref|XP_019079370.1| PREDICTED: thiamine biosynthetic bifunctiona...   657   0.0  
gb|EEC68982.1| hypothetical protein OsI_37743 [Oryza sativa Indi...   659   0.0  
ref|XP_003576978.1| PREDICTED: probable thiamine biosynthetic bi...   658   0.0  

>gb|ONK57653.1| uncharacterized protein A4U43_C09F2700 [Asparagus officinalis]
          Length = 535

 Score =  730 bits (1885), Expect = 0.0
 Identities = 367/424 (86%), Positives = 384/424 (90%), Gaps = 5/424 (1%)
 Frame = -1

Query: 1258 VTAQNTVGV-----QGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIVKVLCNSLR 1094
            V  QN+ G      QGVHPVPEEFVKEQLRSVLSDMNVDVVKTGM+PSVGIVKVLCNSLR
Sbjct: 74   VQGQNSTGPVEGQWQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMIPSVGIVKVLCNSLR 133

Query: 1093 EFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXXXXXXXATV 914
            EFPVRALVVDPVMVSTSGD+LSAPSTLAEYRDELLPMADIVTPNLKE           TV
Sbjct: 134  EFPVRALVVDPVMVSTSGDVLSAPSTLAEYRDELLPMADIVTPNLKEASALLGGISLETV 193

Query: 913  ADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITRNTHGTGCT 734
             DMCSAAKAIHS GPRNVLVKGGDLPDSSDAIDV FDGEQCHEL+GSRIITRNTHGTGCT
Sbjct: 194  GDMCSAAKAIHSFGPRNVLVKGGDLPDSSDAIDVLFDGEQCHELRGSRIITRNTHGTGCT 253

Query: 733  LASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKIKNHEYNAS 554
            LASCIAAELAKGSSILQAV  AK FVESAL YSKD VIGNGPQGPFDHLF++K+  YNA 
Sbjct: 254  LASCIAAELAKGSSILQAVWVAKMFVESALNYSKDIVIGNGPQGPFDHLFRLKSRAYNAG 313

Query: 553  QKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAETREFVEAAT 374
            Q S+F AD+L LYA+TDSRMNKKWGRSITEAVRACIEGGATIVQLREKEA TREFVE+A 
Sbjct: 314  QTSRFNADDLLLYAVTDSRMNKKWGRSITEAVRACIEGGATIVQLREKEANTREFVESAE 373

Query: 373  ACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKIIGVSCKTPA 194
            ACL++CRS GVPLLINDRVDVALACDADGVHVGQSDMPASK RELLGP KI+GVSCKTPA
Sbjct: 374  ACLQICRSHGVPLLINDRVDVALACDADGVHVGQSDMPASKARELLGPRKIVGVSCKTPA 433

Query: 193  QAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGINAGNARSV 14
            QAEQAW+DGADY+GCGGVFPT+TKENNPTLGFDGLKTVC ASKLPVVAIGGINAGNARSV
Sbjct: 434  QAEQAWVDGADYIGCGGVFPTNTKENNPTLGFDGLKTVCLASKLPVVAIGGINAGNARSV 493

Query: 13   MEIG 2
            MEIG
Sbjct: 494  MEIG 497


>ref|XP_008786520.1| PREDICTED: probable thiamine biosynthetic bifunctional enzyme,
            chloroplastic isoform X3 [Phoenix dactylifera]
          Length = 518

 Score =  701 bits (1809), Expect = 0.0
 Identities = 350/432 (81%), Positives = 380/432 (87%)
 Frame = -1

Query: 1297 GALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIV 1118
            GALG YCS+VITAVTAQNTVGVQGVHPVPEEFV EQLRSVLSDM VD+VKTGMLPSVG+V
Sbjct: 46   GALGVYCSSVITAVTAQNTVGVQGVHPVPEEFVAEQLRSVLSDMEVDIVKTGMLPSVGVV 105

Query: 1117 KVLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXX 938
            KVLC+SL+EFPVRALVVDPVMVSTSGD+LS PSTLA YR+EL  MADIVTPNLKE     
Sbjct: 106  KVLCDSLKEFPVRALVVDPVMVSTSGDMLSEPSTLAGYRNELFAMADIVTPNLKEASALL 165

Query: 937  XXXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITR 758
                  TVADMCSAAK+IH+LGPR VLVKGGDLPDSSDAID+FFDGE+CHEL GSRI TR
Sbjct: 166  GGVSLRTVADMCSAAKSIHNLGPRYVLVKGGDLPDSSDAIDIFFDGEECHELYGSRIKTR 225

Query: 757  NTHGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKI 578
            NTHGTGCTLASCIAAELAKGS +L AVQ AK FVESAL YSKD VIGNGPQGPFDHLF++
Sbjct: 226  NTHGTGCTLASCIAAELAKGSPMLHAVQTAKAFVESALNYSKDIVIGNGPQGPFDHLFRL 285

Query: 577  KNHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAET 398
            K+   N   K QF A++LFLYA+TDS MN KW RSIT+AV+A IEGGATI+QLREK+AET
Sbjct: 286  KSQNDNVGLKPQFDANDLFLYAVTDSGMNNKWDRSITDAVKASIEGGATIIQLREKDAET 345

Query: 397  REFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKII 218
            R FVEAA AC+E+CRS GVPLLINDRVDVALACDADGVHVGQSDMPA  VR+LLGP KII
Sbjct: 346  RNFVEAAMACMEICRSHGVPLLINDRVDVALACDADGVHVGQSDMPARVVRKLLGPRKII 405

Query: 217  GVSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGI 38
            GVSCKTPAQAEQAW+DGADY+GCGGVFPT+TKENN T+G DGLK VC ASKLPVVAIGGI
Sbjct: 406  GVSCKTPAQAEQAWIDGADYIGCGGVFPTTTKENNLTVGLDGLKAVCLASKLPVVAIGGI 465

Query: 37   NAGNARSVMEIG 2
              GNA SVM+IG
Sbjct: 466  GPGNAHSVMKIG 477


>ref|XP_008786519.1| PREDICTED: probable thiamine biosynthetic bifunctional enzyme,
            chloroplastic isoform X1 [Phoenix dactylifera]
          Length = 555

 Score =  701 bits (1809), Expect = 0.0
 Identities = 350/432 (81%), Positives = 380/432 (87%)
 Frame = -1

Query: 1297 GALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIV 1118
            GALG YCS+VITAVTAQNTVGVQGVHPVPEEFV EQLRSVLSDM VD+VKTGMLPSVG+V
Sbjct: 83   GALGVYCSSVITAVTAQNTVGVQGVHPVPEEFVAEQLRSVLSDMEVDIVKTGMLPSVGVV 142

Query: 1117 KVLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXX 938
            KVLC+SL+EFPVRALVVDPVMVSTSGD+LS PSTLA YR+EL  MADIVTPNLKE     
Sbjct: 143  KVLCDSLKEFPVRALVVDPVMVSTSGDMLSEPSTLAGYRNELFAMADIVTPNLKEASALL 202

Query: 937  XXXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITR 758
                  TVADMCSAAK+IH+LGPR VLVKGGDLPDSSDAID+FFDGE+CHEL GSRI TR
Sbjct: 203  GGVSLRTVADMCSAAKSIHNLGPRYVLVKGGDLPDSSDAIDIFFDGEECHELYGSRIKTR 262

Query: 757  NTHGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKI 578
            NTHGTGCTLASCIAAELAKGS +L AVQ AK FVESAL YSKD VIGNGPQGPFDHLF++
Sbjct: 263  NTHGTGCTLASCIAAELAKGSPMLHAVQTAKAFVESALNYSKDIVIGNGPQGPFDHLFRL 322

Query: 577  KNHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAET 398
            K+   N   K QF A++LFLYA+TDS MN KW RSIT+AV+A IEGGATI+QLREK+AET
Sbjct: 323  KSQNDNVGLKPQFDANDLFLYAVTDSGMNNKWDRSITDAVKASIEGGATIIQLREKDAET 382

Query: 397  REFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKII 218
            R FVEAA AC+E+CRS GVPLLINDRVDVALACDADGVHVGQSDMPA  VR+LLGP KII
Sbjct: 383  RNFVEAAMACMEICRSHGVPLLINDRVDVALACDADGVHVGQSDMPARVVRKLLGPRKII 442

Query: 217  GVSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGI 38
            GVSCKTPAQAEQAW+DGADY+GCGGVFPT+TKENN T+G DGLK VC ASKLPVVAIGGI
Sbjct: 443  GVSCKTPAQAEQAWIDGADYIGCGGVFPTTTKENNLTVGLDGLKAVCLASKLPVVAIGGI 502

Query: 37   NAGNARSVMEIG 2
              GNA SVM+IG
Sbjct: 503  GPGNAHSVMKIG 514


>ref|XP_010930605.2| PREDICTED: probable thiamine biosynthetic bifunctional enzyme,
            chloroplastic [Elaeis guineensis]
          Length = 556

 Score =  701 bits (1808), Expect = 0.0
 Identities = 348/432 (80%), Positives = 381/432 (88%)
 Frame = -1

Query: 1297 GALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIV 1118
            GALG YCS+VITAVTAQNT+GVQGVHPVPEEFV EQLRSVLSDM VDVVKTGMLPSVG+V
Sbjct: 84   GALGVYCSSVITAVTAQNTIGVQGVHPVPEEFVAEQLRSVLSDMEVDVVKTGMLPSVGVV 143

Query: 1117 KVLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXX 938
            KVLC+SL++FPVRALVVDPVMVSTSGDILS PSTLA YR+EL  MADIVTPNLKE     
Sbjct: 144  KVLCHSLKDFPVRALVVDPVMVSTSGDILSEPSTLAGYRNELFAMADIVTPNLKEASALL 203

Query: 937  XXXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITR 758
                  TVADMCSAAK+IH+LGPR VLVKGGD  DSSDAID+ FDGE+CHEL G+RI TR
Sbjct: 204  GGVSVRTVADMCSAAKSIHNLGPRYVLVKGGDFSDSSDAIDILFDGEECHELYGARIKTR 263

Query: 757  NTHGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKI 578
            NTHGTGCTLASCIAAELAKG+ +LQAVQAAK FVE+AL YSKD V+G+GPQGPFDHLF++
Sbjct: 264  NTHGTGCTLASCIAAELAKGTPMLQAVQAAKAFVETALNYSKDIVMGSGPQGPFDHLFRL 323

Query: 577  KNHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAET 398
            K+  YN  QK QF A +LFLYA+TDS MNKKWGRSIT+AV+  IEGGATI+QLREK+AET
Sbjct: 324  KSQNYNVGQKLQFDAKDLFLYAVTDSGMNKKWGRSITDAVKVSIEGGATIIQLREKDAET 383

Query: 397  REFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKII 218
            R FVEAA AC+E+CRS GVPLLINDRVDVALACDADGVHVGQSDMPA  VR LLGP KII
Sbjct: 384  RNFVEAAMACMEICRSHGVPLLINDRVDVALACDADGVHVGQSDMPARVVRRLLGPRKII 443

Query: 217  GVSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGI 38
            GVSCKTPAQAEQAW+DGADY+GCGGVFPT+TKENN T+G +GLK VC ASKLPVVAIGGI
Sbjct: 444  GVSCKTPAQAEQAWIDGADYIGCGGVFPTTTKENNLTVGLNGLKAVCLASKLPVVAIGGI 503

Query: 37   NAGNARSVMEIG 2
              GNARSVMEIG
Sbjct: 504  GPGNARSVMEIG 515


>ref|XP_020246005.1| probable thiamine biosynthetic bifunctional enzyme, chloroplastic
            isoform X1 [Asparagus officinalis]
          Length = 427

 Score =  688 bits (1775), Expect = 0.0
 Identities = 342/389 (87%), Positives = 358/389 (92%)
 Frame = -1

Query: 1168 MNVDVVKTGMLPSVGIVKVLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELL 989
            MNVDVVKTGM+PSVGIVKVLCNSLREFPVRALVVDPVMVSTSGD+LSAPSTLAEYRDELL
Sbjct: 1    MNVDVVKTGMIPSVGIVKVLCNSLREFPVRALVVDPVMVSTSGDVLSAPSTLAEYRDELL 60

Query: 988  PMADIVTPNLKEXXXXXXXXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVF 809
            PMADIVTPNLKE           TV DMCSAAKAIHS GPRNVLVKGGDLPDSSDAIDV 
Sbjct: 61   PMADIVTPNLKEASALLGGISLETVGDMCSAAKAIHSFGPRNVLVKGGDLPDSSDAIDVL 120

Query: 808  FDGEQCHELQGSRIITRNTHGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKD 629
            FDGEQCHEL+GSRIITRNTHGTGCTLASCIAAELAKGSSILQAV  AK FVESAL YSKD
Sbjct: 121  FDGEQCHELRGSRIITRNTHGTGCTLASCIAAELAKGSSILQAVWVAKMFVESALNYSKD 180

Query: 628  TVIGNGPQGPFDHLFKIKNHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRAC 449
             VIGNGPQGPFDHLF++K+  YNA Q S+F AD+L LYA+TDSRMNKKWGRSITEAVRAC
Sbjct: 181  IVIGNGPQGPFDHLFRLKSRAYNAGQTSRFNADDLLLYAVTDSRMNKKWGRSITEAVRAC 240

Query: 448  IEGGATIVQLREKEAETREFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQS 269
            IEGGATIVQLREKEA TREFVE+A ACL++CRS GVPLLINDRVDVALACDADGVHVGQS
Sbjct: 241  IEGGATIVQLREKEANTREFVESAEACLQICRSHGVPLLINDRVDVALACDADGVHVGQS 300

Query: 268  DMPASKVRELLGPGKIIGVSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGL 89
            DMPASK RELLGP KI+GVSCKTPAQAEQAW+DGADY+GCGGVFPT+TKENNPTLGFDGL
Sbjct: 301  DMPASKARELLGPRKIVGVSCKTPAQAEQAWVDGADYIGCGGVFPTNTKENNPTLGFDGL 360

Query: 88   KTVCSASKLPVVAIGGINAGNARSVMEIG 2
            KTVC ASKLPVVAIGGINAGNARSVMEIG
Sbjct: 361  KTVCLASKLPVVAIGGINAGNARSVMEIG 389


>ref|XP_017697680.1| PREDICTED: probable thiamine biosynthetic bifunctional enzyme,
            chloroplastic isoform X2 [Phoenix dactylifera]
          Length = 553

 Score =  692 bits (1787), Expect = 0.0
 Identities = 348/432 (80%), Positives = 378/432 (87%)
 Frame = -1

Query: 1297 GALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIV 1118
            GALG YCS+VITAVTAQNTVGVQGVHPVPEEFV EQLRSVLSDM VD+VKTGMLPSVG+V
Sbjct: 83   GALGVYCSSVITAVTAQNTVGVQGVHPVPEEFVAEQLRSVLSDMEVDIVKTGMLPSVGVV 142

Query: 1117 KVLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXX 938
            KVLC+SL+EFPVRALVVDPVMVSTSGD+LS PSTLA YR+EL  MADIVTPNLKE     
Sbjct: 143  KVLCDSLKEFPVRALVVDPVMVSTSGDMLSEPSTLAGYRNELFAMADIVTPNLKEASALL 202

Query: 937  XXXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITR 758
                  TVADMCSAAK+IH+LGPR VLVKGGDLPDSSDAID+FFDGE+CHEL GSRI TR
Sbjct: 203  GGVSLRTVADMCSAAKSIHNLGPRYVLVKGGDLPDSSDAIDIFFDGEECHELYGSRIKTR 262

Query: 757  NTHGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKI 578
            NTHGTGCTLASCIAAELAKGS +L AVQ AK FVESAL YSKD VIGNGPQGPFDHLF++
Sbjct: 263  NTHGTGCTLASCIAAELAKGSPMLHAVQTAKAFVESALNYSKDIVIGNGPQGPFDHLFRL 322

Query: 577  KNHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAET 398
            K+   N   K QF A++LFLYA+TDS MN KW RSIT+AV+A IEGGATI+Q  EK+AET
Sbjct: 323  KSQNDNVGLKPQFDANDLFLYAVTDSGMNNKWDRSITDAVKASIEGGATIIQ--EKDAET 380

Query: 397  REFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKII 218
            R FVEAA AC+E+CRS GVPLLINDRVDVALACDADGVHVGQSDMPA  VR+LLGP KII
Sbjct: 381  RNFVEAAMACMEICRSHGVPLLINDRVDVALACDADGVHVGQSDMPARVVRKLLGPRKII 440

Query: 217  GVSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGI 38
            GVSCKTPAQAEQAW+DGADY+GCGGVFPT+TKENN T+G DGLK VC ASKLPVVAIGGI
Sbjct: 441  GVSCKTPAQAEQAWIDGADYIGCGGVFPTTTKENNLTVGLDGLKAVCLASKLPVVAIGGI 500

Query: 37   NAGNARSVMEIG 2
              GNA SVM+IG
Sbjct: 501  GPGNAHSVMKIG 512


>gb|OAY79547.1| putative thiamine biosynthetic bifunctional enzyme, chloroplastic
            [Ananas comosus]
          Length = 514

 Score =  681 bits (1758), Expect = 0.0
 Identities = 337/432 (78%), Positives = 378/432 (87%)
 Frame = -1

Query: 1297 GALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIV 1118
            GALG +CS+V+TAVTAQNTVGVQGVH VPE FV+EQLRSVLSDM+VDVVKTGMLPS+G+V
Sbjct: 42   GALGVFCSSVVTAVTAQNTVGVQGVHVVPESFVEEQLRSVLSDMDVDVVKTGMLPSIGVV 101

Query: 1117 KVLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXX 938
            KVLC+SLR+FPV+ALVVDPVMVSTSGD LSAPSTL+ YRD+L  MADIVTPNLKE     
Sbjct: 102  KVLCDSLRKFPVKALVVDPVMVSTSGDTLSAPSTLSGYRDQLFAMADIVTPNLKEASALL 161

Query: 937  XXXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITR 758
                  TV DM  AAK+I++LGPR+VLVKGGDL DSSDAID+FFDG+ CHEL+GSR+ TR
Sbjct: 162  GGAPLKTVYDMRDAAKSIYNLGPRHVLVKGGDLSDSSDAIDIFFDGQTCHELRGSRVKTR 221

Query: 757  NTHGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKI 578
            NTHGTGCT+ASCIAAELAKGS++LQAVQ AK FVESAL YSKD  IGNGPQGPFDHLF++
Sbjct: 222  NTHGTGCTMASCIAAELAKGSAMLQAVQEAKAFVESALHYSKDMAIGNGPQGPFDHLFRL 281

Query: 577  KNHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAET 398
            K+  YN     +F  D+LFLYA+TDSRMNKKWGRSIT+AV+A IEGGATI+QLREKEAET
Sbjct: 282  KSPHYNVGATPRFNPDDLFLYAVTDSRMNKKWGRSITDAVQAAIEGGATIIQLREKEAET 341

Query: 397  REFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKII 218
            REF+E+A ACLE+CRS  VPLLINDRVDVALAC ADGVHVGQSDMPA   RELLGPGKII
Sbjct: 342  REFIESAKACLEICRSSRVPLLINDRVDVALACGADGVHVGQSDMPARTARELLGPGKII 401

Query: 217  GVSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGI 38
            GVSCKTPAQAE+AW DGADY+GCGGVFPT+TKE+N T+G +GLK VC ASKLPVVAIGGI
Sbjct: 402  GVSCKTPAQAEKAWADGADYIGCGGVFPTTTKEDNITVGMEGLKAVCLASKLPVVAIGGI 461

Query: 37   NAGNARSVMEIG 2
               NARSVMEIG
Sbjct: 462  GIANARSVMEIG 473


>ref|XP_020106624.1| probable thiamine biosynthetic bifunctional enzyme, chloroplastic
            isoform X1 [Ananas comosus]
          Length = 556

 Score =  681 bits (1758), Expect = 0.0
 Identities = 337/432 (78%), Positives = 378/432 (87%)
 Frame = -1

Query: 1297 GALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIV 1118
            GALG +CS+V+TAVTAQNTVGVQGVH VPE FV+EQLRSVLSDM+VDVVKTGMLPS+G+V
Sbjct: 84   GALGVFCSSVVTAVTAQNTVGVQGVHVVPESFVEEQLRSVLSDMDVDVVKTGMLPSIGVV 143

Query: 1117 KVLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXX 938
            KVLC+SLR+FPV+ALVVDPVMVSTSGD LSAPSTL+ YRD+L  MADIVTPNLKE     
Sbjct: 144  KVLCDSLRKFPVKALVVDPVMVSTSGDTLSAPSTLSGYRDQLFAMADIVTPNLKEASALL 203

Query: 937  XXXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITR 758
                  TV DM  AAK+I++LGPR+VLVKGGDL DSSDAID+FFDG+ CHEL+GSR+ TR
Sbjct: 204  GGAPLKTVYDMRDAAKSIYNLGPRHVLVKGGDLSDSSDAIDIFFDGQTCHELRGSRVKTR 263

Query: 757  NTHGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKI 578
            NTHGTGCT+ASCIAAELAKGS++LQAVQ AK FVESAL YSKD  IGNGPQGPFDHLF++
Sbjct: 264  NTHGTGCTMASCIAAELAKGSAMLQAVQEAKAFVESALHYSKDMAIGNGPQGPFDHLFRL 323

Query: 577  KNHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAET 398
            K+  YN     +F  D+LFLYA+TDSRMNKKWGRSIT+AV+A IEGGATI+QLREKEAET
Sbjct: 324  KSPHYNVGATPRFNPDDLFLYAVTDSRMNKKWGRSITDAVQAAIEGGATIIQLREKEAET 383

Query: 397  REFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKII 218
            REF+E+A ACLE+CRS  VPLLINDRVDVALAC ADGVHVGQSDMPA   RELLGPGKII
Sbjct: 384  REFIESAKACLEICRSSRVPLLINDRVDVALACGADGVHVGQSDMPARTARELLGPGKII 443

Query: 217  GVSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGI 38
            GVSCKTPAQAE+AW DGADY+GCGGVFPT+TKE+N T+G +GLK VC ASKLPVVAIGGI
Sbjct: 444  GVSCKTPAQAEKAWADGADYIGCGGVFPTTTKEDNITVGMEGLKAVCLASKLPVVAIGGI 503

Query: 37   NAGNARSVMEIG 2
               NARSVMEIG
Sbjct: 504  GIANARSVMEIG 515


>ref|NP_001105900.2| phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase
            [Zea mays]
 gb|ACG38327.1| phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase
            [Zea mays]
 gb|ONM33769.1| thiamine synthesis3 [Zea mays]
          Length = 551

 Score =  677 bits (1747), Expect = 0.0
 Identities = 336/431 (77%), Positives = 379/431 (87%)
 Frame = -1

Query: 1294 ALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIVK 1115
            ALGAYCS+VITAVTAQNTVGVQG+H VPE+FV EQLRSVLSDM+VDVVKTGMLPS G+VK
Sbjct: 83   ALGAYCSSVITAVTAQNTVGVQGIHAVPEKFVGEQLRSVLSDMSVDVVKTGMLPSAGVVK 142

Query: 1114 VLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXXX 935
            VLC SLR+FPV+ALVVDPVMVSTSGD LS PSTLA YRDEL  MADIVTPN+KE      
Sbjct: 143  VLCESLRKFPVKALVVDPVMVSTSGDTLSGPSTLATYRDELFSMADIVTPNVKEASKLLG 202

Query: 934  XXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITRN 755
                 T++DMC+AA++I+ LGP+ VLVKGGD+PDSSDAIDV FDG++  EL+G RI TRN
Sbjct: 203  DVSLHTISDMCNAAESIYKLGPKYVLVKGGDMPDSSDAIDVLFDGKEFTELRGLRIKTRN 262

Query: 754  THGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKIK 575
            THGTGCTLASCIAAELAKG+++L AVQAAK+FVESAL +SKD VIGNGPQGPFDH F++K
Sbjct: 263  THGTGCTLASCIAAELAKGATMLHAVQAAKKFVESALYHSKDLVIGNGPQGPFDHHFELK 322

Query: 574  NHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAETR 395
            +  Y      +F  D+LFLYA+TDS MNKKWGRSI +AV+A IEGGATIVQLREK+AETR
Sbjct: 323  SPSYKMGSLQKFNPDDLFLYAVTDSGMNKKWGRSIKDAVKAAIEGGATIVQLREKDAETR 382

Query: 394  EFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKIIG 215
            EF+EAA AC+E+C+S GVPLLINDRVDVALACDADGVHVGQSDMPA +VR LLGPGKIIG
Sbjct: 383  EFLEAAKACVEICKSSGVPLLINDRVDVALACDADGVHVGQSDMPAWEVRRLLGPGKIIG 442

Query: 214  VSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGIN 35
            VSCKTPAQAEQAW DGADY+GCGGVFPT+TK NNPTLGF+GL+TVC ASKLPVVAIGGIN
Sbjct: 443  VSCKTPAQAEQAWKDGADYIGCGGVFPTTTKANNPTLGFEGLRTVCLASKLPVVAIGGIN 502

Query: 34   AGNARSVMEIG 2
            AGNA SVME+G
Sbjct: 503  AGNAGSVMELG 513


>gb|PAN16326.1| hypothetical protein PAHAL_C00668 [Panicum hallii]
          Length = 546

 Score =  668 bits (1723), Expect = 0.0
 Identities = 332/431 (77%), Positives = 378/431 (87%)
 Frame = -1

Query: 1294 ALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIVK 1115
            ALGAYCS+VITAVTAQNTVGVQGVH VPEEFV EQLRSVLSDM+VDVVKTGMLPS GIVK
Sbjct: 79   ALGAYCSSVITAVTAQNTVGVQGVHAVPEEFVGEQLRSVLSDMSVDVVKTGMLPSAGIVK 138

Query: 1114 VLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXXX 935
            VLC +LR+FPV+ALVVDPVMVSTSGD LS PSTLA +RDEL PMADIVTPN+KE      
Sbjct: 139  VLCENLRKFPVKALVVDPVMVSTSGDTLSGPSTLATFRDELFPMADIVTPNVKEASKLLG 198

Query: 934  XXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITRN 755
                 T++DM SAA++I+ LGP++VLVKGGD+PDS+DAIDVFFDG++  EL+G RI TRN
Sbjct: 199  DVSLQTISDMRSAAESIYKLGPKHVLVKGGDMPDSTDAIDVFFDGKEFIELRGLRIKTRN 258

Query: 754  THGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKIK 575
            THGTGCTLASCIAAELAKG+++L AV+ AK+FVESAL +SKD VIGNGPQGPFDHLF +K
Sbjct: 259  THGTGCTLASCIAAELAKGATMLHAVEVAKKFVESALYHSKDRVIGNGPQGPFDHLFSLK 318

Query: 574  NHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAETR 395
            +  +      +F  D+LFLYA+TDS MNKKWGRSI +AV+A IEGGATIVQLREK+ ETR
Sbjct: 319  SPLHKMGSLHKFNPDDLFLYAVTDSGMNKKWGRSIKDAVKAAIEGGATIVQLREKDTETR 378

Query: 394  EFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKIIG 215
            EF+EAA AC+++CRS GVPLLINDR+DVALAC+ADGVHVGQSDMPA +VRELLGPGKIIG
Sbjct: 379  EFLEAAKACIDICRSSGVPLLINDRIDVALACNADGVHVGQSDMPAWEVRELLGPGKIIG 438

Query: 214  VSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGIN 35
            VSCKTPAQAEQAW DGADY+G GGVFPT+TK NNPTLGF+GL+ VCSASKLPVVAIGGIN
Sbjct: 439  VSCKTPAQAEQAWKDGADYIGSGGVFPTTTKANNPTLGFEGLRAVCSASKLPVVAIGGIN 498

Query: 34   AGNARSVMEIG 2
             GNA SVME+G
Sbjct: 499  TGNAGSVMELG 509


>ref|XP_004977163.1| probable thiamine biosynthetic bifunctional enzyme, chloroplastic
            [Setaria italica]
 gb|KQK99385.1| hypothetical protein SETIT_009759mg [Setaria italica]
          Length = 546

 Score =  667 bits (1720), Expect = 0.0
 Identities = 331/431 (76%), Positives = 379/431 (87%)
 Frame = -1

Query: 1294 ALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIVK 1115
            ALGAYCS+VITAVTAQNTVGVQGVH VPEEFV EQLRSVLSDM+VDVVKTGMLPS GIVK
Sbjct: 79   ALGAYCSSVITAVTAQNTVGVQGVHAVPEEFVGEQLRSVLSDMSVDVVKTGMLPSAGIVK 138

Query: 1114 VLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXXX 935
            VLC +LR+FPV+ALVVDPVMVSTSGD LS PSTLA Y DEL P+ADIVTPN+KE      
Sbjct: 139  VLCENLRKFPVKALVVDPVMVSTSGDTLSGPSTLATYMDELFPIADIVTPNVKEASKLLG 198

Query: 934  XXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITRN 755
                 T++DM SAA++I+ LGP++VLVKGGD+PDS+DAIDVFFDG++  E +G RI TRN
Sbjct: 199  NVSLQTISDMRSAAESIYKLGPKHVLVKGGDMPDSTDAIDVFFDGKEFIEFRGLRIKTRN 258

Query: 754  THGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKIK 575
            THGTGCTLASCIAAELAKG+++L AV+ AK+FVESAL +SKD VIGNGPQGPFDHLF +K
Sbjct: 259  THGTGCTLASCIAAELAKGATMLHAVEVAKKFVESALYHSKDLVIGNGPQGPFDHLFSLK 318

Query: 574  NHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAETR 395
            +  Y      +F  D+LFLYA+TDS MNKKWGRSI +AV+A IEGGATIVQLREK+A+TR
Sbjct: 319  SPLYKMGSLHKFNPDDLFLYAVTDSGMNKKWGRSIKDAVKAAIEGGATIVQLREKDADTR 378

Query: 394  EFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKIIG 215
            EF+EAA AC+E+CRS GVP+LINDR+DVALAC+ADGVHVGQSDMPA +VRELLGPGKIIG
Sbjct: 379  EFLEAAKACVEICRSSGVPILINDRIDVALACNADGVHVGQSDMPAWEVRELLGPGKIIG 438

Query: 214  VSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGIN 35
            VSCKTPAQA+QAW DGADY+G GGVFPT+TK NNPTLGF+GL++VCSASKLPVVAIGGIN
Sbjct: 439  VSCKTPAQAKQAWKDGADYIGSGGVFPTTTKANNPTLGFEGLRSVCSASKLPVVAIGGIN 498

Query: 34   AGNARSVMEIG 2
            AGNA SVME+G
Sbjct: 499  AGNAGSVMELG 509


>ref|XP_006664409.2| PREDICTED: probable thiamine biosynthetic bifunctional enzyme,
            chloroplastic [Oryza brachyantha]
          Length = 547

 Score =  666 bits (1719), Expect = 0.0
 Identities = 326/431 (75%), Positives = 375/431 (87%)
 Frame = -1

Query: 1294 ALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIVK 1115
            A GAYCS+V+TAVTAQNTVGVQG+H VPEEF++EQL SVLSDM+VDVVKTGMLPS+G+VK
Sbjct: 80   ARGAYCSSVVTAVTAQNTVGVQGIHVVPEEFIREQLNSVLSDMSVDVVKTGMLPSIGVVK 139

Query: 1114 VLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXXX 935
            VLC SL++FPV+ALVVDPVMVSTSGD LS  ST++ YRDEL  MADIVTPN+KE      
Sbjct: 140  VLCESLKKFPVKALVVDPVMVSTSGDTLSESSTISVYRDELFAMADIVTPNVKEASRLLG 199

Query: 934  XXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITRN 755
                 T++DM SAA++I+  GP+ VLVKGGD+P+SS+AID+FFDG++  EL G RI TRN
Sbjct: 200  NVPLHTISDMRSAAESIYKFGPKYVLVKGGDMPESSEAIDIFFDGKEFVELHGHRIQTRN 259

Query: 754  THGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKIK 575
            THGTGCTLASCIA+ELAKG+++L AVQ AK FVESAL +S+D +IGNGPQGPFDHLFK+K
Sbjct: 260  THGTGCTLASCIASELAKGATMLHAVQVAKNFVESALLHSRDLIIGNGPQGPFDHLFKLK 319

Query: 574  NHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAETR 395
               YN   +  FK D LFLYA+TDSRMNKKWGRSI EAV+A IEGGAT+VQLREK++ETR
Sbjct: 320  CPPYNVGSQPSFKPDQLFLYAVTDSRMNKKWGRSIKEAVQAAIEGGATVVQLREKDSETR 379

Query: 394  EFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKIIG 215
            EF+EAA AC+E+C+S GVPLLINDRVD+ALACDADGVHVGQSDM A +VRELLGPGKIIG
Sbjct: 380  EFLEAAKACMEICKSSGVPLLINDRVDIALACDADGVHVGQSDMSAGEVRELLGPGKIIG 439

Query: 214  VSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGIN 35
            VSCKTPAQA+QAW DGADY+GCGGVFPTSTK NNPTLGFDGLKTVC ASKLPVVAIGGIN
Sbjct: 440  VSCKTPAQAQQAWNDGADYIGCGGVFPTSTKANNPTLGFDGLKTVCLASKLPVVAIGGIN 499

Query: 34   AGNARSVMEIG 2
            A NA SVME+G
Sbjct: 500  ASNAGSVMELG 510


>ref|XP_002442970.1| probable thiamine biosynthetic bifunctional enzyme, chloroplastic
            [Sorghum bicolor]
 gb|EES16808.1| hypothetical protein SORBI_3008G067100 [Sorghum bicolor]
          Length = 547

 Score =  664 bits (1714), Expect = 0.0
 Identities = 331/431 (76%), Positives = 377/431 (87%)
 Frame = -1

Query: 1294 ALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIVK 1115
            ALGAYCS+VITAVTAQNTVGVQGVH VPEEFV EQLRSVLSDM+VDVVKTGMLPS G+VK
Sbjct: 80   ALGAYCSSVITAVTAQNTVGVQGVHAVPEEFVGEQLRSVLSDMSVDVVKTGMLPSAGVVK 139

Query: 1114 VLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXXX 935
            VLC SLR+FPV+ALVVDPVMVSTSGD LS PSTLA YRDEL  +ADIVTPN+KE      
Sbjct: 140  VLCESLRKFPVKALVVDPVMVSTSGDTLSGPSTLATYRDELFSVADIVTPNVKEASKLLG 199

Query: 934  XXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITRN 755
                 T++DM +AA++I+ LGP+ VLVKGGD+PDSSDAIDV FDG++  EL+G RI TRN
Sbjct: 200  DVSLHTISDMRNAAESIYKLGPKYVLVKGGDMPDSSDAIDVLFDGKEFTELRGPRIKTRN 259

Query: 754  THGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKIK 575
            THGTGCTLASCIAAELAKG+++L AVQ AK+FVESAL +SKD VIGNGPQGPFDHLF++K
Sbjct: 260  THGTGCTLASCIAAELAKGATMLHAVQVAKKFVESALYHSKDLVIGNGPQGPFDHLFELK 319

Query: 574  NHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAETR 395
            +  Y      +F  DNLFLYA+TDS MNKKW RSI +AV+A IEGGATIVQLREK+A+TR
Sbjct: 320  SPLYKMGSLQKFNPDNLFLYAVTDSGMNKKWDRSIKDAVKAAIEGGATIVQLREKDADTR 379

Query: 394  EFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKIIG 215
            EF+EAA AC+++C+S GVPLLINDR+DVALAC+ADGVHVGQSDMPA +VRELLGPGKIIG
Sbjct: 380  EFLEAAKACVQICKSSGVPLLINDRIDVALACNADGVHVGQSDMPAWEVRELLGPGKIIG 439

Query: 214  VSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGIN 35
            VSCKT  QAEQAW DGADY+GCGGVFPT+TK+NNPTLGF+GL+TVC ASKLPVVAIGGIN
Sbjct: 440  VSCKTLDQAEQAWKDGADYIGCGGVFPTTTKKNNPTLGFEGLRTVCLASKLPVVAIGGIN 499

Query: 34   AGNARSVMEIG 2
            AGNA SVME+G
Sbjct: 500  AGNAGSVMELG 510


>emb|CAJ45026.1| phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase
            [Zea mays]
          Length = 551

 Score =  664 bits (1714), Expect = 0.0
 Identities = 332/431 (77%), Positives = 376/431 (87%)
 Frame = -1

Query: 1294 ALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIVK 1115
            ALGAYCS+ ITAVTAQNTVGVQG+H VPE+FV EQLRSVLSDM+VDVVKTGMLPS G+VK
Sbjct: 83   ALGAYCSSAITAVTAQNTVGVQGIHAVPEKFVGEQLRSVLSDMSVDVVKTGMLPSAGVVK 142

Query: 1114 VLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXXX 935
            VLC SLR+FPV+ALVVDPVMVSTSGD LS PSTLA YRDEL  MADIVTPN+KE      
Sbjct: 143  VLCESLRKFPVKALVVDPVMVSTSGDTLSGPSTLATYRDELFSMADIVTPNVKEASKLLG 202

Query: 934  XXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITRN 755
                 T++DM +AA++I+ LGP+ VLVKGGD+PDSSDAIDV FDG++  EL+G RI TRN
Sbjct: 203  DVSLHTISDMRNAAESIYKLGPKYVLVKGGDMPDSSDAIDVLFDGKEFTELRGLRIKTRN 262

Query: 754  THGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKIK 575
            THGTGCTLASCIAAELAKG+++L AVQAAK+FVESAL +SKD VIGNGPQGPFDHLF++K
Sbjct: 263  THGTGCTLASCIAAELAKGATMLHAVQAAKKFVESALYHSKDLVIGNGPQGPFDHLFELK 322

Query: 574  NHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAETR 395
            +  Y      +F  D+LFLYA+TDS MNKKWGRSI +AV+A IEGGATIVQLREK+AETR
Sbjct: 323  SPSYKMGSLQKFNPDDLFLYAVTDSGMNKKWGRSIKDAVKAAIEGGATIVQLREKDAETR 382

Query: 394  EFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKIIG 215
            EF+EAA AC+E+C+S GVPLLINDRVDVALACDA GVHVGQSD+PA +VR LLGPGKIIG
Sbjct: 383  EFLEAAKACVEICKSLGVPLLINDRVDVALACDAGGVHVGQSDIPAWEVRGLLGPGKIIG 442

Query: 214  VSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGIN 35
            VSCKT AQAEQAW DGADY+GCGGVFPT+TK NNPTLGF+GL+TVC ASKLPVVAIGGIN
Sbjct: 443  VSCKTLAQAEQAWKDGADYIGCGGVFPTTTKANNPTLGFEGLRTVCLASKLPVVAIGGIN 502

Query: 34   AGNARSVMEIG 2
            AGNA SVME+G
Sbjct: 503  AGNAGSVMELG 513


>gb|PIA56493.1| hypothetical protein AQUCO_00700673v1 [Aquilegia coerulea]
          Length = 538

 Score =  661 bits (1706), Expect = 0.0
 Identities = 331/432 (76%), Positives = 365/432 (84%)
 Frame = -1

Query: 1297 GALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIV 1118
            GALG YCSTVITAVTAQNT+GVQGVHPVPE FV EQLRSVLSDM VDVVKTGMLPS+G+V
Sbjct: 70   GALGVYCSTVITAVTAQNTIGVQGVHPVPESFVYEQLRSVLSDMQVDVVKTGMLPSIGVV 129

Query: 1117 KVLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXX 938
            K+LC SL+EFPV+ALVVDPVMVSTSGDIL+ PS L  +R+ELLPMADIVTPNLKE     
Sbjct: 130  KLLCQSLKEFPVQALVVDPVMVSTSGDILADPSILIAFREELLPMADIVTPNLKEASALL 189

Query: 937  XXXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITR 758
                  T+ADMCSAAK+IH LGPR VLVKGGDLP   DA+D+FFDGE CHEL+ SRI T 
Sbjct: 190  GGMQLETIADMCSAAKSIHKLGPRCVLVKGGDLPAPVDAVDIFFDGEHCHELRASRIKTH 249

Query: 757  NTHGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKI 578
            NTHGTGCTLASCIAAELAKGS +L AVQ AKRFVE+AL YSKD  IG+GPQGPFDH  K+
Sbjct: 250  NTHGTGCTLASCIAAELAKGSRMLPAVQVAKRFVETALDYSKDIEIGSGPQGPFDHFSKL 309

Query: 577  KNHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAET 398
            +   Y +S +  F  ++L LYAITDS MNKKW RSIT+AV+A IEGGATIVQLREK+A+T
Sbjct: 310  RRSVYKSSVRRGFDPNDLLLYAITDSGMNKKWARSITDAVKAAIEGGATIVQLREKDADT 369

Query: 397  REFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKII 218
            REF+E A +C+E+C + GVPLLINDRVDVALACDADGVHVGQSDMP   VR LLG GKII
Sbjct: 370  REFLELAKSCVEICHAHGVPLLINDRVDVALACDADGVHVGQSDMPPRIVRALLGSGKII 429

Query: 217  GVSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGI 38
            GVSCKTP QA QAW DGADY+GCGGVFPTSTK NN T+G  GLKTVCSASKLPVVAIGGI
Sbjct: 430  GVSCKTPEQALQAWADGADYIGCGGVFPTSTKANNITVGLSGLKTVCSASKLPVVAIGGI 489

Query: 37   NAGNARSVMEIG 2
            N GNARSVMEIG
Sbjct: 490  NNGNARSVMEIG 501


>ref|XP_009404307.1| PREDICTED: probable thiamine biosynthetic bifunctional enzyme,
            chloroplastic [Musa acuminata subsp. malaccensis]
          Length = 574

 Score =  661 bits (1705), Expect = 0.0
 Identities = 333/431 (77%), Positives = 368/431 (85%)
 Frame = -1

Query: 1297 GALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIV 1118
            GALGAYCS+VITAVTAQNTVGVQGVHPVPE FV EQLRSVLSDM VDVVKTGMLPSVGI+
Sbjct: 103  GALGAYCSSVITAVTAQNTVGVQGVHPVPEAFVAEQLRSVLSDMAVDVVKTGMLPSVGII 162

Query: 1117 KVLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXX 938
            KVLC SL+EFPV+ALVVDPVMVSTSGD+L+ PSTL+ YRDEL  MADIVTPNLKE     
Sbjct: 163  KVLCRSLQEFPVKALVVDPVMVSTSGDMLAGPSTLSGYRDELFAMADIVTPNLKEASALL 222

Query: 937  XXXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITR 758
                  TV+DMCSAAK+IH+LGPRNVLVKGG LP SS+A DVFFDGE  H ++GS + TR
Sbjct: 223  GGMSLRTVSDMCSAAKSIHNLGPRNVLVKGGHLPGSSEATDVFFDGENYHVIRGSHVNTR 282

Query: 757  NTHGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKI 578
            NTHGTGCTLASCIAAELAKGS +LQAVQ AK FVESAL YSKD +IGNGPQGP DHLF +
Sbjct: 283  NTHGTGCTLASCIAAELAKGSPMLQAVQRAKTFVESALDYSKDILIGNGPQGPLDHLFDL 342

Query: 577  KNHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAET 398
              H + A  +S F A +LFLYA+TDS MN+KWGRSI +AV+A IEGGATIVQLR+KEAET
Sbjct: 343  NTHAHTAGARS-FDAKDLFLYAVTDSGMNRKWGRSIVDAVKASIEGGATIVQLRDKEAET 401

Query: 397  REFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKII 218
            R+F+EAA ACLE+CRS GVPLLINDRVDVALACDADGVHVGQSDMPA   R +LGP KII
Sbjct: 402  RDFIEAANACLEICRSNGVPLLINDRVDVALACDADGVHVGQSDMPARVARSILGPQKII 461

Query: 217  GVSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGI 38
            GVSCKT AQA QAW DGADY+GCGGVFPT+TK NN T+G DGLK VC ASK+PVVAIGGI
Sbjct: 462  GVSCKTAAQAAQAWADGADYIGCGGVFPTTTKANNLTVGLDGLKAVCLASKVPVVAIGGI 521

Query: 37   NAGNARSVMEI 5
            +A NA SV+EI
Sbjct: 522  SAANAGSVLEI 532


>emb|CBI29762.3| unnamed protein product, partial [Vitis vinifera]
          Length = 497

 Score =  657 bits (1696), Expect = 0.0
 Identities = 327/431 (75%), Positives = 373/431 (86%)
 Frame = -1

Query: 1294 ALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIVK 1115
            A G YCSTVITAVTAQNTVGVQGV+ VPE+FV EQL+SVLSDM+VDVVKTGMLP++GIVK
Sbjct: 29   ARGVYCSTVITAVTAQNTVGVQGVNIVPEDFVAEQLKSVLSDMHVDVVKTGMLPTIGIVK 88

Query: 1114 VLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXXX 935
            VL +SL+EFPV+ALVVDPVMVSTSGD+L+ PS LA +R+ELLPMADIVTPNLKE      
Sbjct: 89   VLHHSLKEFPVQALVVDPVMVSTSGDVLAGPSILAAFREELLPMADIVTPNLKEASALLG 148

Query: 934  XXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITRN 755
                 TV+DMC+AAK IH +GPRNVLVKGGDLP S DA+D+FFDG+  +EL+ SRI TRN
Sbjct: 149  GLQLETVSDMCTAAKLIHDMGPRNVLVKGGDLPSSLDAVDIFFDGDDFYELRSSRIKTRN 208

Query: 754  THGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKIK 575
            THGTGCTLASCIAAELAKGS IL AV+AAK ++E+AL YSKD  IGNG QGPFDHL K+K
Sbjct: 209  THGTGCTLASCIAAELAKGSQILSAVKAAKHYIETALDYSKDIAIGNGFQGPFDHLLKLK 268

Query: 574  NHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAETR 395
            ++  N+ +K  F   NLFLYA+TDS MNKKWGRSITEAV+A IEGGATIVQLREK+AETR
Sbjct: 269  SNIRNSFRKQAFNPANLFLYAVTDSGMNKKWGRSITEAVKAAIEGGATIVQLREKDAETR 328

Query: 394  EFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKIIG 215
            +F+EAA AC+E+C S GVPLLINDR+DVALACDADGVHVGQSD+PA  VR LLGP KIIG
Sbjct: 329  DFLEAAKACVEICHSHGVPLLINDRIDVALACDADGVHVGQSDIPARVVRTLLGPEKIIG 388

Query: 214  VSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGIN 35
            VSCKTP QAE+AW+DGADY+GCGGV+PT+TK NN T+G DGLKTVC ASKLPVVAIGGIN
Sbjct: 389  VSCKTPEQAEKAWIDGADYIGCGGVYPTNTKANNITVGLDGLKTVCLASKLPVVAIGGIN 448

Query: 34   AGNARSVMEIG 2
            A NAR+VMEIG
Sbjct: 449  ASNARTVMEIG 459


>ref|XP_019079370.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic isoform X2 [Vitis vinifera]
          Length = 511

 Score =  657 bits (1696), Expect = 0.0
 Identities = 327/431 (75%), Positives = 373/431 (86%)
 Frame = -1

Query: 1294 ALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIVK 1115
            A G YCSTVITAVTAQNTVGVQGV+ VPE+FV EQL+SVLSDM+VDVVKTGMLP++GIVK
Sbjct: 43   ARGVYCSTVITAVTAQNTVGVQGVNIVPEDFVAEQLKSVLSDMHVDVVKTGMLPTIGIVK 102

Query: 1114 VLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXXX 935
            VL +SL+EFPV+ALVVDPVMVSTSGD+L+ PS LA +R+ELLPMADIVTPNLKE      
Sbjct: 103  VLHHSLKEFPVQALVVDPVMVSTSGDVLAGPSILAAFREELLPMADIVTPNLKEASALLG 162

Query: 934  XXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITRN 755
                 TV+DMC+AAK IH +GPRNVLVKGGDLP S DA+D+FFDG+  +EL+ SRI TRN
Sbjct: 163  GLQLETVSDMCTAAKLIHDMGPRNVLVKGGDLPSSLDAVDIFFDGDDFYELRSSRIKTRN 222

Query: 754  THGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKIK 575
            THGTGCTLASCIAAELAKGS IL AV+AAK ++E+AL YSKD  IGNG QGPFDHL K+K
Sbjct: 223  THGTGCTLASCIAAELAKGSQILSAVKAAKHYIETALDYSKDIAIGNGFQGPFDHLLKLK 282

Query: 574  NHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAETR 395
            ++  N+ +K  F   NLFLYA+TDS MNKKWGRSITEAV+A IEGGATIVQLREK+AETR
Sbjct: 283  SNIRNSFRKQAFNPANLFLYAVTDSGMNKKWGRSITEAVKAAIEGGATIVQLREKDAETR 342

Query: 394  EFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKIIG 215
            +F+EAA AC+E+C S GVPLLINDR+DVALACDADGVHVGQSD+PA  VR LLGP KIIG
Sbjct: 343  DFLEAAKACVEICHSHGVPLLINDRIDVALACDADGVHVGQSDIPARVVRTLLGPEKIIG 402

Query: 214  VSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGIN 35
            VSCKTP QAE+AW+DGADY+GCGGV+PT+TK NN T+G DGLKTVC ASKLPVVAIGGIN
Sbjct: 403  VSCKTPEQAEKAWIDGADYIGCGGVYPTNTKANNITVGLDGLKTVCLASKLPVVAIGGIN 462

Query: 34   AGNARSVMEIG 2
            A NAR+VMEIG
Sbjct: 463  ASNARTVMEIG 473


>gb|EEC68982.1| hypothetical protein OsI_37743 [Oryza sativa Indica Group]
          Length = 548

 Score =  659 bits (1699), Expect = 0.0
 Identities = 326/431 (75%), Positives = 372/431 (86%)
 Frame = -1

Query: 1294 ALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIVK 1115
            ALGAYCS+V+TAVTAQNT GVQG+H VPEEF++EQL SVLSDM+VDVVKTGMLPS+G+V+
Sbjct: 82   ALGAYCSSVVTAVTAQNTAGVQGIHVVPEEFIREQLNSVLSDMSVDVVKTGMLPSIGVVR 141

Query: 1114 VLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXXX 935
            VLC SL++FPV+ALVVDPVMVSTSGDILS  STL+ YRDEL  MADIVTPN+KE      
Sbjct: 142  VLCESLKKFPVKALVVDPVMVSTSGDILSESSTLSVYRDELFAMADIVTPNVKEASRLLG 201

Query: 934  XXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITRN 755
                 TV+DM +AA++I+  GP++VLVKGGD+ +SSDA DVFFDG++  EL   RI T N
Sbjct: 202  GVSLRTVSDMRNAAESIYKFGPKHVLVKGGDMLESSDATDVFFDGKEFIELHAHRIKTHN 261

Query: 754  THGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKIK 575
            THGTGCTLASCIA+ELAKG+++L AVQ AK FVESAL +SKD VIGNGPQGPFDHLFK+K
Sbjct: 262  THGTGCTLASCIASELAKGATMLHAVQVAKNFVESALHHSKDLVIGNGPQGPFDHLFKLK 321

Query: 574  NHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAETR 395
               YN   +  FK D LFLYA+TDS MNKKWGRSI EAV+A IEGGATIVQLREK++ETR
Sbjct: 322  CPPYNVGSQPSFKPDQLFLYAVTDSGMNKKWGRSIKEAVQAAIEGGATIVQLREKDSETR 381

Query: 394  EFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKIIG 215
            EF+EAA AC+E+C+S GVPLLINDRVD+ALAC+ADGVHVGQSDM A +VRELLGPGKIIG
Sbjct: 382  EFLEAAKACMEICKSSGVPLLINDRVDIALACNADGVHVGQSDMSAHEVRELLGPGKIIG 441

Query: 214  VSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGIN 35
            VSCKTPAQA+QAW DGADY+GCGGVFPTSTK NNPTLGFDGLKTVC ASKLPVVAIGGIN
Sbjct: 442  VSCKTPAQAQQAWNDGADYIGCGGVFPTSTKANNPTLGFDGLKTVCLASKLPVVAIGGIN 501

Query: 34   AGNARSVMEIG 2
            A NA SVME+G
Sbjct: 502  ASNAGSVMELG 512


>ref|XP_003576978.1| PREDICTED: probable thiamine biosynthetic bifunctional enzyme,
            chloroplastic [Brachypodium distachyon]
 gb|KQJ91935.1| hypothetical protein BRADI_4g40670v3 [Brachypodium distachyon]
          Length = 540

 Score =  658 bits (1697), Expect = 0.0
 Identities = 327/431 (75%), Positives = 370/431 (85%)
 Frame = -1

Query: 1294 ALGAYCSTVITAVTAQNTVGVQGVHPVPEEFVKEQLRSVLSDMNVDVVKTGMLPSVGIVK 1115
            ALGAYCS+VITAVTAQNT GVQGVH VPEEF+++QL SVLSDM+VDVVKTGMLPS GIV+
Sbjct: 71   ALGAYCSSVITAVTAQNTAGVQGVHLVPEEFIRQQLNSVLSDMSVDVVKTGMLPSAGIVQ 130

Query: 1114 VLCNSLREFPVRALVVDPVMVSTSGDILSAPSTLAEYRDELLPMADIVTPNLKEXXXXXX 935
            +LC SLR+FPV+ALVVDPVMVSTSGD LS PSTL  YRDEL  MADIVTPN+KE      
Sbjct: 131  ILCESLRKFPVKALVVDPVMVSTSGDALSDPSTLTNYRDELFSMADIVTPNVKEASKLLG 190

Query: 934  XXXXATVADMCSAAKAIHSLGPRNVLVKGGDLPDSSDAIDVFFDGEQCHELQGSRIITRN 755
                 TV+DM  AA +IH  GPR VLVKGGD+PDSS+AID+FFDG++  EL G RI TRN
Sbjct: 191  GIPLLTVSDMRDAAASIHKFGPRYVLVKGGDMPDSSEAIDIFFDGKEFVELHGHRIKTRN 250

Query: 754  THGTGCTLASCIAAELAKGSSILQAVQAAKRFVESALRYSKDTVIGNGPQGPFDHLFKIK 575
            THGTGCTLAS IAAELAKGS++L AV+ AK FVESAL +SKD VIGNGPQGPFDHLF++K
Sbjct: 251  THGTGCTLASSIAAELAKGSTMLHAVRVAKNFVESALHHSKDLVIGNGPQGPFDHLFRLK 310

Query: 574  NHEYNASQKSQFKADNLFLYAITDSRMNKKWGRSITEAVRACIEGGATIVQLREKEAETR 395
               YN   +  F  D+LFLYA+TDSRMNK+W RSI +AV+A IEGGATIVQLREK+AE+R
Sbjct: 311  CPPYNIGSQQMFNPDSLFLYAVTDSRMNKRWDRSIEDAVKAAIEGGATIVQLREKDAESR 370

Query: 394  EFVEAATACLEVCRSRGVPLLINDRVDVALACDADGVHVGQSDMPASKVRELLGPGKIIG 215
            EF+EAA AC+E+C+SRGVPLLINDRVD+ALAC+ADGVHVGQSD+ A +VRELLGPGKIIG
Sbjct: 371  EFLEAANACVEICKSRGVPLLINDRVDIALACNADGVHVGQSDISAREVRELLGPGKIIG 430

Query: 214  VSCKTPAQAEQAWLDGADYVGCGGVFPTSTKENNPTLGFDGLKTVCSASKLPVVAIGGIN 35
            VSCKTPAQAEQAW DGADY+GCGGVFPT+TK NNPTLGF+GLK VC ASKLPVVAIGGIN
Sbjct: 431  VSCKTPAQAEQAWNDGADYIGCGGVFPTTTKANNPTLGFEGLKAVCLASKLPVVAIGGIN 490

Query: 34   AGNARSVMEIG 2
            A NA SVME+G
Sbjct: 491  ATNAGSVMELG 501