BLASTX nr result
ID: Ophiopogon27_contig00013221
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon27_contig00013221
(1056 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_020248753.1| secretory carrier-associated membrane protei... 492 e-174
ref|XP_009385714.1| PREDICTED: secretory carrier-associated memb... 451 e-157
ref|XP_018676808.1| PREDICTED: secretory carrier-associated memb... 443 e-154
ref|XP_019705819.1| PREDICTED: secretory carrier-associated memb... 443 e-154
ref|XP_009409813.1| PREDICTED: secretory carrier-associated memb... 442 e-154
ref|XP_008788581.1| PREDICTED: LOW QUALITY PROTEIN: secretory ca... 438 e-152
ref|NP_001241214.1| uncharacterized protein LOC100780723 [Glycin... 436 e-151
ref|XP_010276767.1| PREDICTED: secretory carrier-associated memb... 435 e-151
ref|XP_009408101.1| PREDICTED: secretory carrier-associated memb... 434 e-151
ref|XP_010261621.1| PREDICTED: secretory carrier-associated memb... 433 e-150
gb|ONK55769.1| uncharacterized protein A4U43_C10F800 [Asparagus ... 432 e-150
ref|XP_020225873.1| secretory carrier-associated membrane protei... 432 e-150
ref|NP_001241252.1| uncharacterized protein LOC100806586 [Glycin... 431 e-149
gb|AFK41920.1| unknown [Lotus japonicus] 429 e-149
gb|PIA58779.1| hypothetical protein AQUCO_00500606v1 [Aquilegia ... 426 e-147
ref|XP_016201200.1| secretory carrier-associated membrane protei... 426 e-147
emb|CDP11723.1| unnamed protein product [Coffea canephora] 426 e-147
ref|XP_015963224.1| secretory carrier-associated membrane protei... 424 e-147
ref|XP_021653706.1| secretory carrier-associated membrane protei... 424 e-147
ref|XP_012069332.1| secretory carrier-associated membrane protei... 424 e-147
>ref|XP_020248753.1| secretory carrier-associated membrane protein 4-like [Asparagus
officinalis]
Length = 266
Score = 492 bits (1267), Expect = e-174
Identities = 240/270 (88%), Positives = 253/270 (93%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSK 830
MQRHDDPNPFDE+ VNPFA +D+SSK RFPGLSSLPFGFG N+NDAT DIPLGS+N SK
Sbjct: 1 MQRHDDPNPFDEEPVNPFAKQSDSSSKPRFPGLSSLPFGFG-NRNDATDDIPLGSVNGSK 59
Query: 829 KKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR 650
K+ L SWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR
Sbjct: 60 GKD--LASWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR 117
Query: 649 MQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYR 470
+QYLAFASWLGI LCLGWNIIAV+VCWIKGGGVKIFLLACIYALLG PLSYVLWYRPLYR
Sbjct: 118 LQYLAFASWLGICLCLGWNIIAVVVCWIKGGGVKIFLLACIYALLGVPLSYVLWYRPLYR 177
Query: 469 AVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIF 290
A++TDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVF GKSLTGILAAIDTF+DH ALVGIF
Sbjct: 178 AMRTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFHGKSLTGILAAIDTFSDH-ALVGIF 236
Query: 289 YLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
YL+GFG FCLE LLSLWV+QKVYMYFRGHK
Sbjct: 237 YLVGFGLFCLETLLSLWVLQKVYMYFRGHK 266
>ref|XP_009385714.1| PREDICTED: secretory carrier-associated membrane protein 4-like
isoform X1 [Musa acuminata subsp. malaccensis]
Length = 266
Score = 451 bits (1160), Expect = e-157
Identities = 213/265 (80%), Positives = 239/265 (90%)
Frame = -3
Query: 994 DPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSKKKEKE 815
DPNPFDE VNPFA SA + S SR P L+S FGFG NKNDATVDIPL ++ND+KK+EKE
Sbjct: 4 DPNPFDEMDVNPFATSAASGSNSRLPTLASKAFGFG-NKNDATVDIPLDTVNDAKKREKE 62
Query: 814 LNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQRMQYLA 635
L +WE +LKRRE DIKRRE+ALT AGV +EEKNWPPFFPIIHHDIAKEIP+HAQR+QYLA
Sbjct: 63 LATWEENLKRREMDIKRREDALTRAGVTIEEKNWPPFFPIIHHDIAKEIPVHAQRLQYLA 122
Query: 634 FASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYRAVKTD 455
FASWLGILLCL WN+IAV+VCWIKGGGVKIF LA IYALLG PLSYVLWYRPLYRA++TD
Sbjct: 123 FASWLGILLCLSWNVIAVIVCWIKGGGVKIFFLAIIYALLGCPLSYVLWYRPLYRAMRTD 182
Query: 454 SAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIFYLIGF 275
SA+KFGWFFL Y+IH+GFCI+AAIAPPIVF GKSLTGILAAIDTF+DH ALVGIFYL+GF
Sbjct: 183 SALKFGWFFLFYLIHVGFCILAAIAPPIVFHGKSLTGILAAIDTFSDH-ALVGIFYLVGF 241
Query: 274 GFFCLEILLSLWVVQKVYMYFRGHK 200
G FCLE L+SLWV+Q+VYMYFRGH+
Sbjct: 242 GLFCLETLISLWVLQRVYMYFRGHE 266
>ref|XP_018676808.1| PREDICTED: secretory carrier-associated membrane protein 4-like
isoform X2 [Musa acuminata subsp. malaccensis]
Length = 264
Score = 443 bits (1140), Expect = e-154
Identities = 212/265 (80%), Positives = 237/265 (89%)
Frame = -3
Query: 994 DPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSKKKEKE 815
DPNPFDE VNPFA SA + S SR P L+S FGFG NKNDATVDIPL + D+KK+EKE
Sbjct: 4 DPNPFDEMDVNPFATSAASGSNSRLPTLASKAFGFG-NKNDATVDIPLDT--DAKKREKE 60
Query: 814 LNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQRMQYLA 635
L +WE +LKRRE DIKRRE+ALT AGV +EEKNWPPFFPIIHHDIAKEIP+HAQR+QYLA
Sbjct: 61 LATWEENLKRREMDIKRREDALTRAGVTIEEKNWPPFFPIIHHDIAKEIPVHAQRLQYLA 120
Query: 634 FASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYRAVKTD 455
FASWLGILLCL WN+IAV+VCWIKGGGVKIF LA IYALLG PLSYVLWYRPLYRA++TD
Sbjct: 121 FASWLGILLCLSWNVIAVIVCWIKGGGVKIFFLAIIYALLGCPLSYVLWYRPLYRAMRTD 180
Query: 454 SAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIFYLIGF 275
SA+KFGWFFL Y+IH+GFCI+AAIAPPIVF GKSLTGILAAIDTF+DH ALVGIFYL+GF
Sbjct: 181 SALKFGWFFLFYLIHVGFCILAAIAPPIVFHGKSLTGILAAIDTFSDH-ALVGIFYLVGF 239
Query: 274 GFFCLEILLSLWVVQKVYMYFRGHK 200
G FCLE L+SLWV+Q+VYMYFRGH+
Sbjct: 240 GLFCLETLISLWVLQRVYMYFRGHE 264
>ref|XP_019705819.1| PREDICTED: secretory carrier-associated membrane protein 4 [Elaeis
guineensis]
Length = 268
Score = 443 bits (1139), Expect = e-154
Identities = 213/270 (78%), Positives = 233/270 (86%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSK 830
M R DDPNPFDE+ VNPF+ A SK P LSSL GFG NK+DATVDIPL + NDSK
Sbjct: 1 MPRRDDPNPFDEEEVNPFSKGAGPGSKLHLPSLSSLHLGFG-NKHDATVDIPLETTNDSK 59
Query: 829 KKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR 650
KK KEL WEADLKRRE DI+RRE+AL++AGV EEKNWPPFFPIIHHDI+KEIPIHAQ+
Sbjct: 60 KKAKELAGWEADLKRREMDIQRREDALSNAGVSTEEKNWPPFFPIIHHDISKEIPIHAQK 119
Query: 649 MQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYR 470
+QYLAFASWLGI+LCL WN+IAV VCWIKGGGVKIF LA IYALLG PLSYVLWYRPLYR
Sbjct: 120 LQYLAFASWLGIVLCLVWNVIAVTVCWIKGGGVKIFFLATIYALLGCPLSYVLWYRPLYR 179
Query: 469 AVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIF 290
A++TDSAVKFGWFFLLY+ HIGFCI AAIAPPIVF GKSLTGILAAIDT +DH LVGIF
Sbjct: 180 AMRTDSAVKFGWFFLLYLFHIGFCIFAAIAPPIVFHGKSLTGILAAIDTISDH-LLVGIF 238
Query: 289 YLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
YL+GFG FCLE L+SLWV+QKVYMYFRG K
Sbjct: 239 YLVGFGLFCLETLISLWVLQKVYMYFRGQK 268
>ref|XP_009409813.1| PREDICTED: secretory carrier-associated membrane protein 4-like [Musa
acuminata subsp. malaccensis]
Length = 268
Score = 442 bits (1137), Expect = e-154
Identities = 208/270 (77%), Positives = 239/270 (88%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSK 830
MQRH DPNPFDED V+PF+N A + KS+FP LSS+ GFG NK+DA VD+PL + NDS+
Sbjct: 1 MQRHGDPNPFDEDAVSPFSNGAGSDKKSKFPSLSSVTLGFG-NKHDAVVDVPLETTNDSR 59
Query: 829 KKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR 650
KK +EL +WEA+LKRRE +IKRRE+AL++AGV E+KNWPP FPIIHHDIAKEIPIHAQR
Sbjct: 60 KKAQELAAWEAELKRRETEIKRREDALSNAGVTTEDKNWPPVFPIIHHDIAKEIPIHAQR 119
Query: 649 MQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYR 470
+QYLAFASWLGI+LCL WNI+AV VCWIKG GVK+F LA IYA LG PLSY+LWYRPLYR
Sbjct: 120 LQYLAFASWLGIVLCLSWNILAVTVCWIKGCGVKVFFLAIIYAFLGCPLSYLLWYRPLYR 179
Query: 469 AVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIF 290
A++T+SAV+FGWFFLLY+IHIGFCIIAAIAPPIVF GKSLTGILAAIDTF+DH +VGIF
Sbjct: 180 AMRTESAVRFGWFFLLYLIHIGFCIIAAIAPPIVFDGKSLTGILAAIDTFSDH-MVVGIF 238
Query: 289 YLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
YLIGFG FCLE LLSLWV+QK+YMYFRGHK
Sbjct: 239 YLIGFGLFCLETLLSLWVLQKIYMYFRGHK 268
>ref|XP_008788581.1| PREDICTED: LOW QUALITY PROTEIN: secretory carrier-associated membrane
protein 4 [Phoenix dactylifera]
Length = 268
Score = 438 bits (1126), Expect = e-152
Identities = 208/270 (77%), Positives = 233/270 (86%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSK 830
M R DDPNPFDE+ VNPF+ A SK FP LSSL GFG NK+DATVDIPL ++NDSK
Sbjct: 1 MPRRDDPNPFDEEEVNPFSKGAGPGSKLHFPSLSSLHLGFG-NKHDATVDIPLETMNDSK 59
Query: 829 KKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR 650
KK KEL +WE DL+RRE DIKRRE+AL+++GV ++EKNWPPFFPIIHHDIAKEIPIHAQ+
Sbjct: 60 KKAKELAAWETDLRRREMDIKRREDALSNSGVSMDEKNWPPFFPIIHHDIAKEIPIHAQK 119
Query: 649 MQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYR 470
+QYLAFASWLGI+LCL WN+IAV VCWIKGGGVKIF LA IYALLG PLSYVLWYRPLYR
Sbjct: 120 LQYLAFASWLGIVLCLVWNVIAVTVCWIKGGGVKIFFLATIYALLGCPLSYVLWYRPLYR 179
Query: 469 AVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIF 290
A++TDSAVKFGWF Y+ HIGFCI AAIAPPIVF GKSLTGILAA+DT +DH LVGIF
Sbjct: 180 AMRTDSAVKFGWFSFXYLFHIGFCIFAAIAPPIVFHGKSLTGILAAVDTISDH-LLVGIF 238
Query: 289 YLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
YL+GFG FCLE L+SLWV+QKVYMYFRG K
Sbjct: 239 YLVGFGLFCLETLISLWVLQKVYMYFRGQK 268
>ref|NP_001241214.1| uncharacterized protein LOC100780723 [Glycine max]
gb|ACU20966.1| unknown [Glycine max]
gb|KHN40405.1| Secretory carrier-associated membrane protein 4 [Glycine soja]
gb|KRH74289.1| hypothetical protein GLYMA_01G010300 [Glycine max]
Length = 269
Score = 436 bits (1121), Expect = e-151
Identities = 206/271 (76%), Positives = 238/271 (87%), Gaps = 1/271 (0%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADT-SSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDS 833
M RH DPNPF+E+ VNPF+N +SKSR P L+S GFG ++DATVDIPL + NDS
Sbjct: 1 MNRHHDPNPFEEEEVNPFSNGTTAPASKSRIPPLASESLGFG-QRHDATVDIPLDTTNDS 59
Query: 832 KKKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQ 653
KKK +EL +WEADLKRRE++IKRREEA++ AGVPV++KNWPPFFPIIHHDIA EIP+HAQ
Sbjct: 60 KKKGQELAAWEADLKRREKEIKRREEAVSRAGVPVDDKNWPPFFPIIHHDIANEIPVHAQ 119
Query: 652 RMQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLY 473
R+QYLAFASWLGI+LCLG+N++AV VCWI+GGGVKIF LA IYALLG PLSYVLWYRPLY
Sbjct: 120 RLQYLAFASWLGIVLCLGFNVVAVTVCWIRGGGVKIFFLAVIYALLGVPLSYVLWYRPLY 179
Query: 472 RAVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGI 293
RA++TDSA+KFGWFF+LY++HIGFCI AAIAPPIVF GKSLTGILAAID F+DH LVGI
Sbjct: 180 RAMRTDSALKFGWFFMLYLLHIGFCIFAAIAPPIVFHGKSLTGILAAIDVFSDH-VLVGI 238
Query: 292 FYLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
FYLIGFG FCLE LLS WV+QK+YMYFRGHK
Sbjct: 239 FYLIGFGMFCLEALLSFWVLQKIYMYFRGHK 269
>ref|XP_010276767.1| PREDICTED: secretory carrier-associated membrane protein 4-like
isoform X1 [Nelumbo nucifera]
Length = 268
Score = 435 bits (1118), Expect = e-151
Identities = 205/270 (75%), Positives = 234/270 (86%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSK 830
M R DDPNPF+E+ VNPFAN + +SKSR P GFG +K+DATVDIPL ++ND K
Sbjct: 1 MNRRDDPNPFEEEEVNPFANGSAPASKSRIPSFLPQSLGFG-HKHDATVDIPLDTMNDPK 59
Query: 829 KKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR 650
K+EKEL +WEADLKRREQDIKRRE+A+ G+P ++KNWPPFFPIIHHDIA EIPIHAQR
Sbjct: 60 KREKELAAWEADLKRREQDIKRREDAVARVGIPTDDKNWPPFFPIIHHDIANEIPIHAQR 119
Query: 649 MQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYR 470
+QYLAFASWLGI+LCL +NI+AV+VCWIKGGGVKIF LA IYALLG PLSYVLWYRPLYR
Sbjct: 120 LQYLAFASWLGIVLCLVFNILAVVVCWIKGGGVKIFFLATIYALLGCPLSYVLWYRPLYR 179
Query: 469 AVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIF 290
A++TDSA+KFGWFFL Y++HIGFCI +AIAPPIVF GKSLTGILAAID F+DH LVGIF
Sbjct: 180 AMRTDSALKFGWFFLFYLLHIGFCIFSAIAPPIVFHGKSLTGILAAIDVFSDH-VLVGIF 238
Query: 289 YLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
YLIGFG FCLE LLSLWV+QK+YMYFRG K
Sbjct: 239 YLIGFGLFCLESLLSLWVLQKIYMYFRGQK 268
>ref|XP_009408101.1| PREDICTED: secretory carrier-associated membrane protein 4-like
[Musa acuminata subsp. malaccensis]
Length = 266
Score = 434 bits (1116), Expect = e-151
Identities = 206/265 (77%), Positives = 234/265 (88%)
Frame = -3
Query: 994 DPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSKKKEKE 815
DPNPFDE+ VNPFA+ + S SR L+S FGFG NKNDATVDIPL ++ND+KK E E
Sbjct: 4 DPNPFDEEDVNPFASGSAPGSNSRLSTLASKAFGFG-NKNDATVDIPLDTVNDAKKSETE 62
Query: 814 LNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQRMQYLA 635
L +WE +LKRRE DIKRRE++LT AGV VE+KNWP FFPIIHHDIAKEIP+HAQR+QYLA
Sbjct: 63 LAAWEENLKRREMDIKRREDSLTRAGVTVEDKNWPSFFPIIHHDIAKEIPVHAQRLQYLA 122
Query: 634 FASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYRAVKTD 455
FASWLGI+LCL WN+IAV+VCWI+GGGVKIF LA IYALLG PLSYVLWYRPLYRA++T+
Sbjct: 123 FASWLGIVLCLSWNVIAVIVCWIRGGGVKIFFLAIIYALLGCPLSYVLWYRPLYRAMRTN 182
Query: 454 SAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIFYLIGF 275
SA KFGWFFL Y+IHIGFCI+AAIAPPIVF GKSLTGILAAIDTF+DH ALVGIFYL+GF
Sbjct: 183 SAFKFGWFFLFYLIHIGFCILAAIAPPIVFHGKSLTGILAAIDTFSDH-ALVGIFYLVGF 241
Query: 274 GFFCLEILLSLWVVQKVYMYFRGHK 200
G FCLEIL+SLWV+QKVYMYFRG +
Sbjct: 242 GLFCLEILISLWVLQKVYMYFRGQQ 266
>ref|XP_010261621.1| PREDICTED: secretory carrier-associated membrane protein 4 [Nelumbo
nucifera]
Length = 268
Score = 433 bits (1113), Expect = e-150
Identities = 205/270 (75%), Positives = 233/270 (86%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSK 830
M R DDPNPFDE+ VNPFA A +SKSR L GFG +K+DATVDIPL + ND K
Sbjct: 1 MNRRDDPNPFDEEEVNPFAKGAAPASKSRLTSLIPESLGFG-HKHDATVDIPLDTTNDIK 59
Query: 829 KKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR 650
K++KE+ +WEADLKRREQDIKRRE+A+ AGVP +++NWPPFFPIIHHDIA EIPIHAQR
Sbjct: 60 KRQKEIAAWEADLKRREQDIKRREDAVARAGVPTDDRNWPPFFPIIHHDIANEIPIHAQR 119
Query: 649 MQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYR 470
+QYLAFASWLGI+LCL +N++A++VCWIKGGGVKIF LA IYALLG PLSYVLWYRPLYR
Sbjct: 120 LQYLAFASWLGIVLCLVFNVLAIVVCWIKGGGVKIFFLATIYALLGCPLSYVLWYRPLYR 179
Query: 469 AVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIF 290
A++TDSA+KF WFFL Y++HIGFCI AAIAPPIVF GKSLTGILAAID F+DH VGIF
Sbjct: 180 AMRTDSALKFSWFFLFYLLHIGFCIFAAIAPPIVFHGKSLTGILAAIDVFSDH-VFVGIF 238
Query: 289 YLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
YLIGFGFFCLE LLSLWV+QKVYMYFRGHK
Sbjct: 239 YLIGFGFFCLESLLSLWVLQKVYMYFRGHK 268
>gb|ONK55769.1| uncharacterized protein A4U43_C10F800 [Asparagus officinalis]
Length = 239
Score = 432 bits (1110), Expect = e-150
Identities = 212/239 (88%), Positives = 224/239 (93%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSK 830
MQRHDDPNPFDE+ VNPFA +D+SSK RFPGLSSLPFGFG N+NDAT DIPLGS+N SK
Sbjct: 1 MQRHDDPNPFDEEPVNPFAKQSDSSSKPRFPGLSSLPFGFG-NRNDATDDIPLGSVNGSK 59
Query: 829 KKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR 650
K+ L SWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR
Sbjct: 60 GKD--LASWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR 117
Query: 649 MQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYR 470
+QYLAFASWLGI LCLGWNIIAV+VCWIKGGGVKIFLLACIYALLG PLSYVLWYRPLYR
Sbjct: 118 LQYLAFASWLGICLCLGWNIIAVVVCWIKGGGVKIFLLACIYALLGVPLSYVLWYRPLYR 177
Query: 469 AVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGI 293
A++TDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVF GKSLTGILAAIDTF+DH ALVG+
Sbjct: 178 AMRTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFHGKSLTGILAAIDTFSDH-ALVGV 235
>ref|XP_020225873.1| secretory carrier-associated membrane protein 4 [Cajanus cajan]
Length = 269
Score = 432 bits (1111), Expect = e-150
Identities = 203/271 (74%), Positives = 239/271 (88%), Gaps = 1/271 (0%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANS-ADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDS 833
M RH DPNPFDE+ VNPF+N A +SKSR P ++S P GFG ++DATVDIPL + NDS
Sbjct: 1 MNRHHDPNPFDEEEVNPFSNGGAAPASKSRVPPVASEPLGFG-QRHDATVDIPLDNTNDS 59
Query: 832 KKKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQ 653
KKK +EL +WEADLKRRE++IK+REEA++ AGVPV++KNWPPFFPIIHHDIA EIP+HAQ
Sbjct: 60 KKKGQELAAWEADLKRREKEIKKREEAVSRAGVPVDDKNWPPFFPIIHHDIANEIPVHAQ 119
Query: 652 RMQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLY 473
R+QYLAFASWLGI+LCL +N++AV+VCWI+GGGVKIF LA IYALLG PLSYVLWYRPLY
Sbjct: 120 RLQYLAFASWLGIVLCLVFNVVAVIVCWIRGGGVKIFFLAVIYALLGVPLSYVLWYRPLY 179
Query: 472 RAVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGI 293
RA++TDSA+KFGWFF+ Y++HI FC+ AAIAPPIVF GKSLTGILAAID F+DH LVGI
Sbjct: 180 RAMRTDSALKFGWFFMFYLLHIVFCVFAAIAPPIVFHGKSLTGILAAIDVFSDH-VLVGI 238
Query: 292 FYLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
FYL+GFG FCLE LLSLWV+QK+YMYFRGHK
Sbjct: 239 FYLVGFGMFCLEALLSLWVLQKIYMYFRGHK 269
>ref|NP_001241252.1| uncharacterized protein LOC100806586 [Glycine max]
gb|ACU19390.1| unknown [Glycine max]
gb|KRH39631.1| hypothetical protein GLYMA_09G210800 [Glycine max]
Length = 269
Score = 431 bits (1109), Expect = e-149
Identities = 203/271 (74%), Positives = 238/271 (87%), Gaps = 1/271 (0%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANS-ADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDS 833
M RH+DPNPF+E+ VNPF+N A +SKSR P L+S P GFG ++DATVDIPL + NDS
Sbjct: 1 MNRHNDPNPFEEEEVNPFSNGGAAPASKSRIPPLASQPLGFG-QRHDATVDIPLDTTNDS 59
Query: 832 KKKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQ 653
KK+ +EL +WEADLKR+E++IKRREEA++ AGVPV++KNWPP FPIIHHDIA EIP+HAQ
Sbjct: 60 KKRGQELAAWEADLKRKEKEIKRREEAVSRAGVPVDDKNWPPIFPIIHHDIANEIPVHAQ 119
Query: 652 RMQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLY 473
R+QYLAFASWLGI+LCL +N++AV VCWI+GGGVKIF LA IY LLG PLSYVLWYRPLY
Sbjct: 120 RLQYLAFASWLGIVLCLVFNVVAVTVCWIRGGGVKIFFLAVIYGLLGVPLSYVLWYRPLY 179
Query: 472 RAVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGI 293
RA++TDSA+KFGWFF+ Y++HIGFCI AAIAPPIVF GKSLTGILAAID F+DH LVGI
Sbjct: 180 RAMRTDSALKFGWFFMFYLLHIGFCIFAAIAPPIVFHGKSLTGILAAIDVFSDH-VLVGI 238
Query: 292 FYLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
FYLIGFG FCLE LLSLWV+QK+YMYFRGHK
Sbjct: 239 FYLIGFGMFCLEALLSLWVLQKIYMYFRGHK 269
>gb|AFK41920.1| unknown [Lotus japonicus]
Length = 268
Score = 429 bits (1104), Expect = e-149
Identities = 198/270 (73%), Positives = 235/270 (87%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSK 830
M RH DPNPFDED VNPF+N A SKSR P L+S P GFG ++DATVDIPL + N+ K
Sbjct: 1 MNRHHDPNPFDEDEVNPFSNGAAPGSKSRIPPLASEPLGFG-QRHDATVDIPLDTTNEPK 59
Query: 829 KKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR 650
KK +EL +WEADLKRRE+D+KRRE+A++ AGVPV++KNWPP FPIIHHDIA EIP+HAQR
Sbjct: 60 KKGQELAAWEADLKRREKDLKRREDAVSRAGVPVDDKNWPPIFPIIHHDIANEIPVHAQR 119
Query: 649 MQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYR 470
+QYLAFASWLGI+LCL +N++AV+VCWI+GGGVKIF LA IYALLG PLS+VLWYRPLYR
Sbjct: 120 LQYLAFASWLGIVLCLVFNVVAVMVCWIRGGGVKIFFLAVIYALLGVPLSHVLWYRPLYR 179
Query: 469 AVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIF 290
A++TDSA+KFGWFF+ Y++HI FCI AAIAPP+VF GKSLTGILAA D F+DH LVGIF
Sbjct: 180 AMRTDSALKFGWFFMFYLLHIAFCIFAAIAPPVVFHGKSLTGILAATDVFSDH-VLVGIF 238
Query: 289 YLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
YL+GFG FCLE LLSLWV+QK+Y++FRGHK
Sbjct: 239 YLVGFGLFCLESLLSLWVIQKIYLFFRGHK 268
>gb|PIA58779.1| hypothetical protein AQUCO_00500606v1 [Aquilegia coerulea]
Length = 268
Score = 426 bits (1096), Expect = e-147
Identities = 202/270 (74%), Positives = 235/270 (87%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSK 830
M R +DPNPFDE+ VNPFAN+A +SKSR P + S GFG K+DATVDIPL +++DSK
Sbjct: 1 MNRRNDPNPFDEEGVNPFANAAAPASKSRIPQIVSDTLGFG-RKHDATVDIPLDTMSDSK 59
Query: 829 KKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQR 650
KEK+L +WEADL+RREQDIKRRE+A+T AGVPV+++NWPPFFPIIHHDI EIP+HAQR
Sbjct: 60 NKEKDLANWEADLRRREQDIKRREDAVTRAGVPVDDRNWPPFFPIIHHDITNEIPVHAQR 119
Query: 649 MQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYR 470
+QYLAFASWLGI+LCL +NIIAV VCWI+G GV+IF LA IYALLG PLSYVLWYRPLYR
Sbjct: 120 LQYLAFASWLGIVLCLVFNIIAVTVCWIEGEGVRIFFLATIYALLGVPLSYVLWYRPLYR 179
Query: 469 AVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIF 290
A++TDSA+KF WFFL Y++HIGFCI AAIAPPIVF+GKSLTGILAAID F+DH L GIF
Sbjct: 180 AMRTDSALKFSWFFLFYLLHIGFCIFAAIAPPIVFRGKSLTGILAAIDVFSDHR-LAGIF 238
Query: 289 YLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
YLIGF FCLE LLSLWV+QK+Y+YFRGHK
Sbjct: 239 YLIGFALFCLESLLSLWVLQKIYLYFRGHK 268
>ref|XP_016201200.1| secretory carrier-associated membrane protein 4 isoform X2 [Arachis
ipaensis]
Length = 268
Score = 426 bits (1096), Expect = e-147
Identities = 205/271 (75%), Positives = 233/271 (85%), Gaps = 1/271 (0%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADT-SSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDS 833
M RH DPNPF+E+ VNPF+N A SKSR P LSS P GFG K+DATVDIPL DS
Sbjct: 1 MNRHQDPNPFEEE-VNPFSNGAGAPGSKSRIPQLSSEPVGFG-QKHDATVDIPLDVTTDS 58
Query: 832 KKKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQ 653
KKK +EL +WEADLKRRE++IKRREEA+ AGVPV++KNWPPFFPIIHHDIA EIP+HAQ
Sbjct: 59 KKKGQELAAWEADLKRREKEIKRREEAVAKAGVPVDDKNWPPFFPIIHHDIANEIPVHAQ 118
Query: 652 RMQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLY 473
R+QYLAFASWLGI+LCL +N++AV+VCWI+GGG KIF LA IYALLG PLSYVLWYRPLY
Sbjct: 119 RLQYLAFASWLGIVLCLVFNVVAVIVCWIRGGGSKIFFLAVIYALLGVPLSYVLWYRPLY 178
Query: 472 RAVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGI 293
RA+ TDSA+KFGWFFL Y+IHI FCI AAIAPP+VF GKSLTGILAAID F+DH LVGI
Sbjct: 179 RAMSTDSALKFGWFFLFYLIHIAFCIFAAIAPPVVFHGKSLTGILAAIDVFSDH-VLVGI 237
Query: 292 FYLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
FYL+GFG FCLE LLSLWV+QK+Y+YFRGHK
Sbjct: 238 FYLVGFGLFCLEALLSLWVLQKIYLYFRGHK 268
>emb|CDP11723.1| unnamed protein product [Coffea canephora]
Length = 271
Score = 426 bits (1096), Expect = e-147
Identities = 207/273 (75%), Positives = 234/273 (85%), Gaps = 3/273 (1%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDH--VNPFAN-SADTSSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLN 839
M R DPNPFDE+ VNPF+ +A SKSR P S GFG K+DATVDIPL S+N
Sbjct: 1 MNRSSDPNPFDEEEQEVNPFSKGNAAPGSKSRVPQFVSNTLGFG-QKHDATVDIPLDSMN 59
Query: 838 DSKKKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIH 659
DSKKK+KEL WEAD+KRRE+DIKRRE+A+ SAGVP +++NWPPFFPIIHHDIA EIP+H
Sbjct: 60 DSKKKQKELADWEADIKRRERDIKRREDAVASAGVPADDRNWPPFFPIIHHDIANEIPVH 119
Query: 658 AQRMQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRP 479
AQR+QYLAFASWLGI+LCL +N+IAV VCWI+GGGVKIF L+ IYALLG PLSYVLWYRP
Sbjct: 120 AQRLQYLAFASWLGIVLCLVFNVIAVTVCWIRGGGVKIFFLSIIYALLGCPLSYVLWYRP 179
Query: 478 LYRAVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALV 299
LYRA++TDSA KFGWFFL Y+IHIGFCI+AAIAPPIVF G+SLTGILAAID F+DH LV
Sbjct: 180 LYRAMRTDSAFKFGWFFLCYLIHIGFCILAAIAPPIVFHGRSLTGILAAIDVFSDH-VLV 238
Query: 298 GIFYLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
GIFYL+GFG FCLE LLSLWVVQKVYMYFRGHK
Sbjct: 239 GIFYLVGFGLFCLESLLSLWVVQKVYMYFRGHK 271
>ref|XP_015963224.1| secretory carrier-associated membrane protein 4 isoform X2 [Arachis
duranensis]
Length = 268
Score = 424 bits (1091), Expect = e-147
Identities = 203/271 (74%), Positives = 233/271 (85%), Gaps = 1/271 (0%)
Frame = -3
Query: 1009 MQRHDDPNPFDEDHVNPFANSADT-SSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDS 833
M RH DPNPF+E+ VNPF+N A SKSR P +SS P GFG ++DATVDIPL DS
Sbjct: 1 MNRHQDPNPFEEE-VNPFSNGAGAPGSKSRIPQMSSEPVGFG-QRHDATVDIPLDVTTDS 58
Query: 832 KKKEKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQ 653
KKK +EL +WEADLKRRE++IKRREEA+ AGVPV++KNWPPFFPIIHHDIA EIP+HAQ
Sbjct: 59 KKKGQELAAWEADLKRREKEIKRREEAVAKAGVPVDDKNWPPFFPIIHHDIANEIPVHAQ 118
Query: 652 RMQYLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLY 473
R+QYLAFASWLGI+LCL +N++AV+VCWI+GGG KIF LA IYALLG PLSYVLWYRPLY
Sbjct: 119 RLQYLAFASWLGIVLCLVFNVVAVIVCWIRGGGSKIFFLAVIYALLGVPLSYVLWYRPLY 178
Query: 472 RAVKTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGI 293
RA+ TDSA+KFGWFFL Y+IHI FCI AAIAPP+VF GKSLTGILAAID F+DH LVGI
Sbjct: 179 RAMSTDSALKFGWFFLFYLIHIAFCIFAAIAPPVVFHGKSLTGILAAIDVFSDH-VLVGI 237
Query: 292 FYLIGFGFFCLEILLSLWVVQKVYMYFRGHK 200
FYL+GFG FCLE LLSLWV+QK+Y+YFRGHK
Sbjct: 238 FYLVGFGLFCLEALLSLWVLQKIYLYFRGHK 268
>ref|XP_021653706.1| secretory carrier-associated membrane protein 4 [Hevea brasiliensis]
Length = 269
Score = 424 bits (1090), Expect = e-147
Identities = 204/268 (76%), Positives = 230/268 (85%), Gaps = 1/268 (0%)
Frame = -3
Query: 1000 HDDPNPFDEDHVNPFANSADTS-SKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSKKK 824
H DPNPFDE+ VNPF+N A S SK+R P L P GFG NDATVDIPL ++ND KKK
Sbjct: 5 HHDPNPFDEEEVNPFSNGATASVSKTRIPPLGPEPTGFG--HNDATVDIPLDTMNDPKKK 62
Query: 823 EKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQRMQ 644
EKEL +WEADLKRRE++IKRRE+A+ AGVP + KNWPPFFPIIHHDIA EIPIHAQR+Q
Sbjct: 63 EKELVAWEADLKRREKEIKRREDAVAKAGVPADNKNWPPFFPIIHHDIANEIPIHAQRLQ 122
Query: 643 YLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYRAV 464
YLAFASWLGI+LCL +N+IAVLV WI+GGGVKIFLLA IYAL G P+SYVLWYRPLYRA+
Sbjct: 123 YLAFASWLGIVLCLVFNVIAVLVNWIRGGGVKIFLLASIYALSGVPMSYVLWYRPLYRAM 182
Query: 463 KTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIFYL 284
+TDSA+KFGWFFL Y++HIGFCI AAIAPPIVF GKSLTGIL AID +DH LVGIFYL
Sbjct: 183 RTDSALKFGWFFLFYLVHIGFCIFAAIAPPIVFHGKSLTGILPAIDVISDH-LLVGIFYL 241
Query: 283 IGFGFFCLEILLSLWVVQKVYMYFRGHK 200
+GFG FCLE LLSLWV+QK+YMYFRGHK
Sbjct: 242 VGFGLFCLESLLSLWVLQKIYMYFRGHK 269
>ref|XP_012069332.1| secretory carrier-associated membrane protein 4 [Jatropha curcas]
gb|KDP40432.1| hypothetical protein JCGZ_03847 [Jatropha curcas]
Length = 269
Score = 424 bits (1090), Expect = e-147
Identities = 202/268 (75%), Positives = 232/268 (86%), Gaps = 1/268 (0%)
Frame = -3
Query: 1000 HDDPNPFDEDHVNPFANSADT-SSKSRFPGLSSLPFGFGGNKNDATVDIPLGSLNDSKKK 824
H DPNPFDE+ VNPF+N A SK+R P + + P GFG NDATVDIPL ++ND KKK
Sbjct: 5 HSDPNPFDEEEVNPFSNGATAPGSKTRVPPVGAQPAGFG--HNDATVDIPLDTMNDPKKK 62
Query: 823 EKELNSWEADLKRREQDIKRREEALTSAGVPVEEKNWPPFFPIIHHDIAKEIPIHAQRMQ 644
EKEL +WEADLKRRE+DIKRRE+A+ +AGVP ++KNWPPFFPIIHHDIA EIPIHAQR+Q
Sbjct: 63 EKELAAWEADLKRREKDIKRREDAVANAGVPADDKNWPPFFPIIHHDIANEIPIHAQRLQ 122
Query: 643 YLAFASWLGILLCLGWNIIAVLVCWIKGGGVKIFLLACIYALLGWPLSYVLWYRPLYRAV 464
YLAFASWLGI+LCL +N+IAVLV WI+GGGVKIFLLA IYAL G P+SYVLWYRPLYRA+
Sbjct: 123 YLAFASWLGIVLCLVFNVIAVLVNWIRGGGVKIFLLASIYALSGIPMSYVLWYRPLYRAM 182
Query: 463 KTDSAVKFGWFFLLYMIHIGFCIIAAIAPPIVFQGKSLTGILAAIDTFNDHNALVGIFYL 284
+TDSA+KFGWFFL Y++H+GFCI AAIAPPIVF GKSLTGIL A+D +D N LVGIFYL
Sbjct: 183 RTDSALKFGWFFLFYLLHVGFCIFAAIAPPIVFHGKSLTGILPAVDVISD-NLLVGIFYL 241
Query: 283 IGFGFFCLEILLSLWVVQKVYMYFRGHK 200
+GFG FCLE LLSLWV+QKVYMYFRGHK
Sbjct: 242 VGFGLFCLESLLSLWVLQKVYMYFRGHK 269