BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon27_contig00009913
         (686 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020276958.1| uncharacterized protein LOC109851309 [Aspara...   393   e-133
ref|XP_020099746.1| uncharacterized protein LOC109718135 [Ananas...   339   e-112
ref|XP_008801937.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   339   e-112
ref|XP_020590686.1| uncharacterized protein LOC110031690 [Phalae...   337   e-111
gb|PKI60196.1| hypothetical protein CRG98_019384 [Punica granatum]    333   e-111
gb|OWM73493.1| hypothetical protein CDL15_Pgr026592 [Punica gran...   335   e-110
ref|XP_009407701.1| PREDICTED: uncharacterized protein LOC103990...   332   e-110
ref|XP_009407675.1| PREDICTED: uncharacterized protein LOC103990...   332   e-109
gb|OAY68223.1| tRNA-specific 2-thiouridylase MnmA, partial [Anan...   331   e-109
ref|XP_010906766.1| PREDICTED: uncharacterized protein LOC105033...   330   e-108
gb|PKA59941.1| tRNA-specific 2-thiouridylase [Apostasia shenzhen...   330   e-108
dbj|BAS82567.1| Os03g0174600, partial [Oryza sativa Japonica Group]   323   e-108
ref|XP_019155295.1| PREDICTED: uncharacterized protein LOC109152...   326   e-108
ref|XP_017983487.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   325   e-108
ref|XP_012446852.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   328   e-108
gb|POE53180.1| trna-specific 2-thiouridylase mnma [Quercus suber]     327   e-108
gb|PHT38568.1| hypothetical protein CQW23_22141 [Capsicum baccatum]   328   e-108
ref|XP_016565613.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   328   e-107
ref|XP_003558694.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   327   e-107
ref|XP_019263360.1| PREDICTED: uncharacterized protein LOC109241...   327   e-107

>ref|XP_020276958.1| uncharacterized protein LOC109851309 [Asparagus officinalis]
 gb|ONK59753.1| tRNA-specific 2-thiouridylase MnmA [Asparagus officinalis]
          Length = 485

 Score =  393 bits (1010), Expect = e-133
 Identities = 184/207 (88%), Positives = 197/207 (95%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGKVKFSEFVAKHIGE+EGI+LEAETGDYLG+HRGFWFYT+GQRQGLRLPGGPWY
Sbjct: 279 QGICFLGKVKFSEFVAKHIGEQEGIILEAETGDYLGDHRGFWFYTVGQRQGLRLPGGPWY 338

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTNNQLRCKVRHGPNFHNCSI 325
           VVEKDVQNNVVFVSRNYYSLDK+RR FRVGT KWISG PP  +QLRCKVRHGP+FHNCSI
Sbjct: 339 VVEKDVQNNVVFVSRNYYSLDKKRRKFRVGTLKWISGSPPKKSQLRCKVRHGPSFHNCSI 398

Query: 324 RTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKAL 145
             ESDD+GYGEVVTV+LSEDDQGLAAGQFAAFYD D CIGSG+ILDSWDDKSFPICAKAL
Sbjct: 399 TMESDDTGYGEVVTVDLSEDDQGLAAGQFAAFYDGDSCIGSGVILDSWDDKSFPICAKAL 458

Query: 144 EIAQMEDKSKLGKPVRILNQDTADCKR 64
           EIAQ++DKSKLGKPVRI NQ+TADCKR
Sbjct: 459 EIAQLQDKSKLGKPVRIFNQETADCKR 485


>ref|XP_020099746.1| uncharacterized protein LOC109718135 [Ananas comosus]
          Length = 495

 Score =  339 bits (869), Expect = e-112
 Identities = 159/200 (79%), Positives = 176/200 (88%), Gaps = 1/200 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGKVKFSEFVAKHIGE EG+LLEAETGDYLG HRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 292 QGICFLGKVKFSEFVAKHIGEMEGVLLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWY 351

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTNN-QLRCKVRHGPNFHNCS 328
           VVEKD QNNVVFVSRNY+SLDKRRR F VG+  W SG PP NN QL+CKVRHGP F+NC+
Sbjct: 352 VVEKDAQNNVVFVSRNYFSLDKRRRTFHVGSLNWFSGSPPANNRQLQCKVRHGPGFYNCT 411

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           I  ES +    + + V+LSEDDQGLAAGQFAAFYD D+CIGSG+ILDSWD+K FPIC +A
Sbjct: 412 ITKESIEDDNKDSLVVHLSEDDQGLAAGQFAAFYDGDVCIGSGIILDSWDEKCFPICERA 471

Query: 147 LEIAQMEDKSKLGKPVRILN 88
           LE+A+MEDKSKLGKPVRI+N
Sbjct: 472 LELAKMEDKSKLGKPVRIMN 491


>ref|XP_008801937.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA [Phoenix dactylifera]
          Length = 500

 Score =  339 bits (869), Expect = e-112
 Identities = 163/202 (80%), Positives = 178/202 (88%), Gaps = 1/202 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGKVKF EFVAK IGE EGILLEAETGDYLG HRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 297 QGICFLGKVKFCEFVAKQIGEVEGILLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWY 356

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPT-NNQLRCKVRHGPNFHNCS 328
           VVEKDVQNNVVFVSRNY+SLDKRRR FRVG+  W SG PP+ N QL+CKVRHGP F++CS
Sbjct: 357 VVEKDVQNNVVFVSRNYFSLDKRRRTFRVGSLNWFSGSPPSENEQLQCKVRHGPGFYDCS 416

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           I   S   G+ EVV V++SEDDQGLAAGQFAAFY +DIC+GSG+ILDSWDDKSFPIC KA
Sbjct: 417 ITEWSSKDGHEEVVEVHISEDDQGLAAGQFAAFYCNDICLGSGVILDSWDDKSFPICTKA 476

Query: 147 LEIAQMEDKSKLGKPVRILNQD 82
           LEIA+MEDKSKLGKPV+I N +
Sbjct: 477 LEIAKMEDKSKLGKPVKIKNAE 498


>ref|XP_020590686.1| uncharacterized protein LOC110031690 [Phalaenopsis equestris]
          Length = 510

 Score =  337 bits (864), Expect = e-111
 Identities = 158/210 (75%), Positives = 183/210 (87%), Gaps = 4/210 (1%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGKVKFSEFVA+HIGE EGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 290 QGICFLGKVKFSEFVARHIGEMEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 349

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTN-NQLRCKVRHGPNFHNCS 328
           VVEKDVQNNVVFVSRNY+SLDKRRR FR+G+ +W SG PP N +QLRCKVRHGP F+ CS
Sbjct: 350 VVEKDVQNNVVFVSRNYFSLDKRRRTFRIGSLRWFSGSPPENKDQLRCKVRHGPGFYGCS 409

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           IR E+  +G  ++  ++LSEDDQGLAAGQF AFY+ +IC+GSG+ILDSWDD  FP+C+KA
Sbjct: 410 IRLETTGNGTEQIAAIHLSEDDQGLAAGQFTAFYEDNICLGSGVILDSWDDLYFPVCSKA 469

Query: 147 LEIAQMEDKSKLGKPVRILN---QDTADCK 67
           LEIA+M++KS LG+P+RILN   + TA  K
Sbjct: 470 LEIARMKEKSNLGRPIRILNLTSESTASLK 499


>gb|PKI60196.1| hypothetical protein CRG98_019384 [Punica granatum]
          Length = 408

 Score =  333 bits (854), Expect = e-111
 Identities = 150/197 (76%), Positives = 178/197 (90%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGK+KFS+FVA+HIGEKEG++LEAE+GDYLGNHRGFWFYTIGQRQGL LPGGPWY
Sbjct: 204 QGICFLGKIKFSDFVARHIGEKEGVILEAESGDYLGNHRGFWFYTIGQRQGLNLPGGPWY 263

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTNNQLRCKVRHGPNFHNCSI 325
           VVEKDV+NNVVFVSRNYYSLDK+RR+FRVG+ KW+SG+PP ++QL+CKVRHGP+F++C++
Sbjct: 264 VVEKDVRNNVVFVSRNYYSLDKKRRLFRVGSMKWLSGVPPHSSQLQCKVRHGPSFYDCNL 323

Query: 324 RTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKAL 145
             ES   G  +V  V LSEDDQGLAAGQF AFYD   C+GSG+IL+SWDD+ FP+C KAL
Sbjct: 324 TIESGKDGGEDVGVVQLSEDDQGLAAGQFGAFYDGRTCVGSGVILESWDDRGFPVCEKAL 383

Query: 144 EIAQMEDKSKLGKPVRI 94
           EIA+MEDKSKLGKPV+I
Sbjct: 384 EIARMEDKSKLGKPVKI 400


>gb|OWM73493.1| hypothetical protein CDL15_Pgr026592 [Punica granatum]
          Length = 488

 Score =  335 bits (859), Expect = e-110
 Identities = 151/202 (74%), Positives = 181/202 (89%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGK+KFS+FVA+HIGEKEG++LEAE+GDYLGNHRGFWFYTIGQRQGL LPGGPWY
Sbjct: 275 QGICFLGKIKFSDFVARHIGEKEGVILEAESGDYLGNHRGFWFYTIGQRQGLNLPGGPWY 334

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTNNQLRCKVRHGPNFHNCSI 325
           VVEKDV+NNVVFVSRNYYSLDK+RR+FRVG+ KW+SG+PP ++QL+CKVRHGP+F++C++
Sbjct: 335 VVEKDVRNNVVFVSRNYYSLDKKRRLFRVGSMKWLSGVPPHSSQLQCKVRHGPSFYDCNL 394

Query: 324 RTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKAL 145
             ES   G  +V  V LSEDDQGLAAGQF AFYD   C+GSG+IL+SWDD+ FP+C KAL
Sbjct: 395 TIESGKDGGEDVGVVQLSEDDQGLAAGQFGAFYDGRTCVGSGVILESWDDRGFPVCEKAL 454

Query: 144 EIAQMEDKSKLGKPVRILNQDT 79
           EIA+MEDKSKLGKPV+I N ++
Sbjct: 455 EIARMEDKSKLGKPVKIKNVES 476


>ref|XP_009407701.1| PREDICTED: uncharacterized protein LOC103990311 isoform X2 [Musa
           acuminata subsp. malaccensis]
          Length = 405

 Score =  332 bits (851), Expect = e-110
 Identities = 158/203 (77%), Positives = 175/203 (86%), Gaps = 1/203 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGKVKFSEFVAKHIGE EGILLEAETGDYLGNH GFWFYTIGQRQGLRL GGPWY
Sbjct: 202 QGICFLGKVKFSEFVAKHIGELEGILLEAETGDYLGNHHGFWFYTIGQRQGLRLSGGPWY 261

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTN-NQLRCKVRHGPNFHNCS 328
           VVEKDV+NNVVFVSRNY+SLDKRRR F VG+  W SG PP N  QL+CKVRHGP F++CS
Sbjct: 262 VVEKDVRNNVVFVSRNYFSLDKRRRTFHVGSLNWFSGAPPENTEQLQCKVRHGPGFYDCS 321

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           I       G  + + V+LSEDDQGLAAGQFAAFY  D+CIGSG+I+DSWD+K FPIC+KA
Sbjct: 322 IMAVPGIDGDEDNLVVHLSEDDQGLAAGQFAAFYHGDVCIGSGVIMDSWDEKCFPICSKA 381

Query: 147 LEIAQMEDKSKLGKPVRILNQDT 79
           LEIA+MEDKSKLGKP+RI+N DT
Sbjct: 382 LEIARMEDKSKLGKPIRIMNPDT 404


>ref|XP_009407675.1| PREDICTED: uncharacterized protein LOC103990311 isoform X1 [Musa
           acuminata subsp. malaccensis]
 ref|XP_018682618.1| PREDICTED: uncharacterized protein LOC103990311 isoform X1 [Musa
           acuminata subsp. malaccensis]
 ref|XP_018682619.1| PREDICTED: uncharacterized protein LOC103990311 isoform X1 [Musa
           acuminata subsp. malaccensis]
          Length = 506

 Score =  332 bits (851), Expect = e-109
 Identities = 158/203 (77%), Positives = 175/203 (86%), Gaps = 1/203 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGKVKFSEFVAKHIGE EGILLEAETGDYLGNH GFWFYTIGQRQGLRL GGPWY
Sbjct: 303 QGICFLGKVKFSEFVAKHIGELEGILLEAETGDYLGNHHGFWFYTIGQRQGLRLSGGPWY 362

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTN-NQLRCKVRHGPNFHNCS 328
           VVEKDV+NNVVFVSRNY+SLDKRRR F VG+  W SG PP N  QL+CKVRHGP F++CS
Sbjct: 363 VVEKDVRNNVVFVSRNYFSLDKRRRTFHVGSLNWFSGAPPENTEQLQCKVRHGPGFYDCS 422

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           I       G  + + V+LSEDDQGLAAGQFAAFY  D+CIGSG+I+DSWD+K FPIC+KA
Sbjct: 423 IMAVPGIDGDEDNLVVHLSEDDQGLAAGQFAAFYHGDVCIGSGVIMDSWDEKCFPICSKA 482

Query: 147 LEIAQMEDKSKLGKPVRILNQDT 79
           LEIA+MEDKSKLGKP+RI+N DT
Sbjct: 483 LEIARMEDKSKLGKPIRIMNPDT 505


>gb|OAY68223.1| tRNA-specific 2-thiouridylase MnmA, partial [Ananas comosus]
          Length = 486

 Score =  331 bits (848), Expect = e-109
 Identities = 155/195 (79%), Positives = 171/195 (87%), Gaps = 1/195 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGKVKFSEFVAKHIGE EG+LLEAETGDYLG HRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 292 QGICFLGKVKFSEFVAKHIGEMEGVLLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWY 351

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTNN-QLRCKVRHGPNFHNCS 328
           VVEKD QNNVVFVSRNY+SLDKRRR F VG+  W SG PP NN QL+CKVRHGP F+NC+
Sbjct: 352 VVEKDAQNNVVFVSRNYFSLDKRRRTFHVGSLNWFSGSPPANNRQLQCKVRHGPGFYNCT 411

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           I  ES +    + + V+LSEDDQGLAAGQFAAFYD D+CIGSG+ILDSWD+K FPIC +A
Sbjct: 412 ITKESIEDDNKDSLVVHLSEDDQGLAAGQFAAFYDGDVCIGSGIILDSWDEKCFPICERA 471

Query: 147 LEIAQMEDKSKLGKP 103
           LE+A+MEDKSKLGKP
Sbjct: 472 LELAKMEDKSKLGKP 486


>ref|XP_010906766.1| PREDICTED: uncharacterized protein LOC105033599 [Elaeis guineensis]
          Length = 495

 Score =  330 bits (847), Expect = e-108
 Identities = 159/200 (79%), Positives = 177/200 (88%), Gaps = 1/200 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGKVKFSEFVAKHIGE EGILLEAETGDYLG H+GFWFYTIGQRQGLRLPGGPWY
Sbjct: 292 QGICFLGKVKFSEFVAKHIGEMEGILLEAETGDYLGKHQGFWFYTIGQRQGLRLPGGPWY 351

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTNNQ-LRCKVRHGPNFHNCS 328
           VVEKDVQNNVVFVSRNY+SLDKRRR F VG+  W SG PP+NN+ L+CKVRHGP F++CS
Sbjct: 352 VVEKDVQNNVVFVSRNYFSLDKRRRTFCVGSLNWFSGSPPSNNELLQCKVRHGPGFYDCS 411

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           I   S  +G  +V+ V++SEDDQGLAAGQFAAFY +DIC+GSG+ILDSWDDKSF I  KA
Sbjct: 412 IAEWSSKNGQEDVIEVHISEDDQGLAAGQFAAFYCNDICLGSGVILDSWDDKSFCISTKA 471

Query: 147 LEIAQMEDKSKLGKPVRILN 88
           LEIA+MEDKSKLGKPV+I N
Sbjct: 472 LEIAKMEDKSKLGKPVKIKN 491


>gb|PKA59941.1| tRNA-specific 2-thiouridylase [Apostasia shenzhenica]
          Length = 493

 Score =  330 bits (845), Expect = e-108
 Identities = 152/200 (76%), Positives = 177/200 (88%), Gaps = 1/200 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGKVKFSEF+AKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 290 QGICFLGKVKFSEFIAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 349

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISG-LPPTNNQLRCKVRHGPNFHNCS 328
           VVEKDV+NNVVFVSR+Y+SL+KRR +FRVG+ KW SG  P  ++++RCKVRHGPNF++CS
Sbjct: 350 VVEKDVKNNVVFVSRDYFSLEKRRCMFRVGSLKWFSGSAPDDHDEVRCKVRHGPNFYDCS 409

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           I+    D G  E+  V+LSEDDQGLAAGQFAAFY  ++C+GSG+ILDSWDDK FP+C KA
Sbjct: 410 IKVGQSDDGTEEIAVVHLSEDDQGLAAGQFAAFYHENVCLGSGIILDSWDDKCFPVCQKA 469

Query: 147 LEIAQMEDKSKLGKPVRILN 88
           L IA ++DKS LG+P+RILN
Sbjct: 470 LGIASLQDKSALGRPIRILN 489


>dbj|BAS82567.1| Os03g0174600, partial [Oryza sativa Japonica Group]
          Length = 310

 Score =  323 bits (827), Expect = e-108
 Identities = 151/200 (75%), Positives = 174/200 (87%), Gaps = 1/200 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGKVKFSEFV + IGE EG+LLEAETGDYLG HRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 97  QGICFLGKVKFSEFVERQIGEMEGVLLEAETGDYLGTHRGFWFYTIGQRQGLRLPGGPWY 156

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTNN-QLRCKVRHGPNFHNCS 328
           VVEK VQNNVVFVSRNYYSLDKRRR FRVG+  W S   PTNN QL+CKVRH P FH+C+
Sbjct: 157 VVEKGVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFSNSGPTNNEQLKCKVRHSPEFHDCT 216

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           + T+   S +G ++ V LSEDDQGLAAGQFAAFY  ++C+GSG+ILDSWD+ +FP+CA+A
Sbjct: 217 V-TQEQTSEHGVILVVRLSEDDQGLAAGQFAAFYRDNLCLGSGIILDSWDEMNFPVCARA 275

Query: 147 LEIAQMEDKSKLGKPVRILN 88
           LEIA+MEDKS+LGKPV+I+N
Sbjct: 276 LEIARMEDKSRLGKPVKIMN 295


>ref|XP_019155295.1| PREDICTED: uncharacterized protein LOC109152183 isoform X2 [Ipomoea
           nil]
          Length = 418

 Score =  326 bits (836), Expect = e-108
 Identities = 153/210 (72%), Positives = 179/210 (85%), Gaps = 1/210 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGK+KFSEFVA+HIGE+EGI+LEAETGDYLG HRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 163 QGICFLGKIKFSEFVARHIGEEEGIILEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWY 222

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTN-NQLRCKVRHGPNFHNCS 328
           VVEKD  NNVVFVSRNY+S+DK+RR+FRVG+ KW+SG  P N  QL+CKVRHGPNF+NCS
Sbjct: 223 VVEKDTTNNVVFVSRNYFSVDKKRRLFRVGSLKWLSGSSPGNITQLQCKVRHGPNFYNCS 282

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           +  E DD+G  E+  V L+EDDQGLAAGQFAAFYD   CIGSG+IL+SWDD+ +P+C +A
Sbjct: 283 LAMEVDDNGQ-EIAVVQLTEDDQGLAAGQFAAFYDGRACIGSGVILESWDDQGYPVCERA 341

Query: 147 LEIAQMEDKSKLGKPVRILNQDTADCKR*H 58
           LEIA+MEDKS LGKPV+I+ +     K  H
Sbjct: 342 LEIARMEDKSMLGKPVKIMVKPENSLKESH 371


>ref|XP_017983487.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA isoform X2 [Theobroma
           cacao]
          Length = 386

 Score =  325 bits (833), Expect = e-108
 Identities = 151/198 (76%), Positives = 174/198 (87%), Gaps = 1/198 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGK++FS+FVA+HIGE EGI+LEAETGD+LG HRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 163 QGICFLGKIRFSDFVARHIGEMEGIILEAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWY 222

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTN-NQLRCKVRHGPNFHNCS 328
           VVEKDV+NNVVFVSRNY+SLDKRRR+FRVG+ KW+SG PP +  QL+CKVRHGP F+NCS
Sbjct: 223 VVEKDVENNVVFVSRNYFSLDKRRRLFRVGSLKWLSGSPPNHIKQLQCKVRHGPGFYNCS 282

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
               S + G  +V  V L EDDQGLAAGQFAAFY+   CIGSG+IL+SWDD+ FP+CAKA
Sbjct: 283 FELVSGEYGSEDVAVVCLHEDDQGLAAGQFAAFYEGQTCIGSGVILESWDDQGFPVCAKA 342

Query: 147 LEIAQMEDKSKLGKPVRI 94
           LEIA+MEDKSKLGKPV+I
Sbjct: 343 LEIARMEDKSKLGKPVKI 360


>ref|XP_012446852.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Gossypium
           raimondii]
 gb|KJB55374.1| hypothetical protein B456_009G073000 [Gossypium raimondii]
          Length = 490

 Score =  328 bits (842), Expect = e-108
 Identities = 149/201 (74%), Positives = 178/201 (88%), Gaps = 1/201 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGK++FS+FVA+HIGE EGI+LEAETGD+LG HRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 285 QGICFLGKIRFSDFVARHIGEMEGIILEAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWY 344

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTN-NQLRCKVRHGPNFHNCS 328
           VV+KDV+NNVVFVSRNY+SLDKRRRVFRVG+ KW SG PP + +QL+CKVRHGP F+NC+
Sbjct: 345 VVQKDVKNNVVFVSRNYFSLDKRRRVFRVGSLKWFSGSPPNHIDQLQCKVRHGPGFYNCN 404

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
            + E  + G  ++  V LSEDDQGLAAGQFAAFY+   CIGSG+IL+SWDD+ FP+CAKA
Sbjct: 405 FKMEHGEDGNDDIAVVQLSEDDQGLAAGQFAAFYEGQTCIGSGVILESWDDRGFPVCAKA 464

Query: 147 LEIAQMEDKSKLGKPVRILNQ 85
           LEIA+MEDKS+LGKPV+I+ +
Sbjct: 465 LEIARMEDKSELGKPVKIMTK 485


>gb|POE53180.1| trna-specific 2-thiouridylase mnma [Quercus suber]
          Length = 463

 Score =  327 bits (839), Expect = e-108
 Identities = 148/198 (74%), Positives = 174/198 (87%), Gaps = 1/198 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGK+KFSEFV +HIGE+EG++LEAETGD+LG HRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 204 QGICFLGKIKFSEFVGRHIGEREGVILEAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWY 263

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTN-NQLRCKVRHGPNFHNCS 328
           VVEKD++NNVVFVSRNY+S DKRRR+FRVG+ KW+SGLPP    QL+CKVRHGP F+NCS
Sbjct: 264 VVEKDIKNNVVFVSRNYFSFDKRRRIFRVGSIKWLSGLPPNQLCQLQCKVRHGPGFYNCS 323

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           +  E    G  ++  V+LSEDDQGLAAGQFAAFY    C+GSG+IL+SWDD+ FP+CAKA
Sbjct: 324 LEMELGQDGGEDIAVVHLSEDDQGLAAGQFAAFYQGRTCLGSGIILESWDDQGFPVCAKA 383

Query: 147 LEIAQMEDKSKLGKPVRI 94
           LEIA+MEDKSKLGKP++I
Sbjct: 384 LEIARMEDKSKLGKPIKI 401


>gb|PHT38568.1| hypothetical protein CQW23_22141 [Capsicum baccatum]
          Length = 488

 Score =  328 bits (840), Expect = e-108
 Identities = 152/199 (76%), Positives = 180/199 (90%), Gaps = 1/199 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGK+KFSEFVA+HIGE EG++LEAE+GDYLGNHRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 281 QGICFLGKIKFSEFVARHIGESEGVILEAESGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 340

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTN-NQLRCKVRHGPNFHNCS 328
           VVEKD++NNVV+VSRNY+S+DK+RR+FRVG+ KW+SGL P   ++L+CKVRHGP F+NCS
Sbjct: 341 VVEKDIRNNVVYVSRNYFSVDKKRRLFRVGSLKWLSGLLPRQISELQCKVRHGPGFYNCS 400

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           +  E D+ G  EV  V LSEDDQGLAAGQFAAFYD  ICIGSG+IL+SWDD+ +PICA+A
Sbjct: 401 LVVEVDEHG-REVAVVRLSEDDQGLAAGQFAAFYDRRICIGSGIILESWDDQGYPICARA 459

Query: 147 LEIAQMEDKSKLGKPVRIL 91
           LEIA+MEDKSKLGKPV+I+
Sbjct: 460 LEIARMEDKSKLGKPVKIM 478


>ref|XP_016565613.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA [Capsicum annuum]
 gb|PHT86308.1| hypothetical protein T459_08414 [Capsicum annuum]
          Length = 492

 Score =  328 bits (840), Expect = e-107
 Identities = 152/199 (76%), Positives = 180/199 (90%), Gaps = 1/199 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGK+KFSEFVA+HIGE EG++LEAE+GDYLGNHRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 285 QGICFLGKIKFSEFVARHIGESEGVILEAESGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 344

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTN-NQLRCKVRHGPNFHNCS 328
           VVEKD++NNVV+VSRNY+S+DK+RR+FRVG+ KW+SGL P   ++L+CKVRHGP F+NCS
Sbjct: 345 VVEKDIRNNVVYVSRNYFSVDKKRRLFRVGSLKWLSGLLPRQISELQCKVRHGPGFYNCS 404

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           +  E D+ G  EV  V LSEDDQGLAAGQFAAFYD  ICIGSG+IL+SWDD+ +PICA+A
Sbjct: 405 LVVEVDEHG-REVAVVRLSEDDQGLAAGQFAAFYDRRICIGSGIILESWDDQGYPICARA 463

Query: 147 LEIAQMEDKSKLGKPVRIL 91
           LEIA+MEDKSKLGKPV+I+
Sbjct: 464 LEIARMEDKSKLGKPVKIM 482


>ref|XP_003558694.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA [Brachypodium
           distachyon]
 gb|KQK23363.1| hypothetical protein BRADI_1g72967v3 [Brachypodium distachyon]
          Length = 481

 Score =  327 bits (838), Expect = e-107
 Identities = 155/200 (77%), Positives = 173/200 (86%), Gaps = 1/200 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGKVKFSEFV +HIGE EG+LLEAETGDYLG HRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 268 QGICFLGKVKFSEFVERHIGEMEGVLLEAETGDYLGRHRGFWFYTIGQRQGLRLPGGPWY 327

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTNN-QLRCKVRHGPNFHNCS 328
           VVEKDVQNNVVFVSRNYYSLDKRRR FRVG+  W S   P N+ QL+CKVRH P FHNC+
Sbjct: 328 VVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFSDSGPENDEQLKCKVRHSPEFHNCT 387

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           +  E  +   G+V+ V+LSEDDQGLAAGQFAAFY  + C+GSG+ILDSWD+ SFP+CA A
Sbjct: 388 VTREQTEES-GDVLAVHLSEDDQGLAAGQFAAFYRDNTCLGSGIILDSWDEMSFPVCANA 446

Query: 147 LEIAQMEDKSKLGKPVRILN 88
           LEIA+MEDKSKLGKPVRI+N
Sbjct: 447 LEIARMEDKSKLGKPVRIVN 466


>ref|XP_019263360.1| PREDICTED: uncharacterized protein LOC109241098 [Nicotiana
           attenuata]
 gb|OIT37212.1| hypothetical protein A4A49_08038 [Nicotiana attenuata]
          Length = 488

 Score =  327 bits (838), Expect = e-107
 Identities = 149/199 (74%), Positives = 179/199 (89%), Gaps = 1/199 (0%)
 Frame = -3

Query: 684 QGICFLGKVKFSEFVAKHIGEKEGILLEAETGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 505
           QGICFLGK+KFSEFVA+HIGE EG++LEAE+GDYLGNHRGFWFYTIGQRQGLRLPGGPWY
Sbjct: 281 QGICFLGKIKFSEFVARHIGESEGVILEAESGDYLGNHRGFWFYTIGQRQGLRLPGGPWY 340

Query: 504 VVEKDVQNNVVFVSRNYYSLDKRRRVFRVGTPKWISGLPPTN-NQLRCKVRHGPNFHNCS 328
           VVEKD++NNVV+VSRNY+S+DK+RR+FRVG+ KW+SGLPP   +QL+CKVRHGP F+NCS
Sbjct: 341 VVEKDIKNNVVYVSRNYFSVDKKRRLFRVGSLKWLSGLPPRQISQLQCKVRHGPGFYNCS 400

Query: 327 IRTESDDSGYGEVVTVNLSEDDQGLAAGQFAAFYDSDICIGSGLILDSWDDKSFPICAKA 148
           +  E D+ G  EV  V +SEDDQGLAAGQFAAFYD   CIGSG+IL+SWDD+ +PIC +A
Sbjct: 401 LAMEVDEHGQ-EVAVVRISEDDQGLAAGQFAAFYDGRTCIGSGVILESWDDQGYPICERA 459

Query: 147 LEIAQMEDKSKLGKPVRIL 91
           +EIA+MEDKS+LGKPV+I+
Sbjct: 460 IEIARMEDKSRLGKPVKIM 478