BLASTX nr result
ID: Ophiopogon27_contig00008879
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon27_contig00008879
(2206 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_017604286.1| PREDICTED: RNA-directed DNA polymerase homol... 97 9e-20
ref|XP_017701988.1| PREDICTED: RNA-directed DNA polymerase homol... 95 2e-18
ref|XP_015075579.1| PREDICTED: RNA-directed DNA polymerase homol... 94 4e-18
ref|XP_019066470.1| PREDICTED: uncharacterized protein LOC109118... 93 5e-18
ref|XP_012441962.1| PREDICTED: uncharacterized protein LOC105766... 100 6e-18
ref|XP_021607608.1| uncharacterized protein LOC110611529 [Maniho... 100 6e-18
ref|XP_016675550.1| PREDICTED: RNA-directed DNA polymerase homol... 93 7e-18
ref|XP_016675304.1| PREDICTED: RNA-directed DNA polymerase homol... 93 2e-17
ref|XP_016556474.1| PREDICTED: RNA-directed DNA polymerase homol... 93 2e-17
ref|XP_016752415.1| PREDICTED: RNA-directed DNA polymerase homol... 90 2e-17
gb|EOY20476.1| Uncharacterized protein TCM_046344 [Theobroma cacao] 98 2e-17
gb|OMO91869.1| reverse transcriptase [Corchorus capsularis] 99 3e-17
gb|PPY92755.1| hypothetical protein C5P31_25760, partial [Escher... 94 5e-17
ref|XP_016743303.1| PREDICTED: RNA-directed DNA polymerase homol... 91 5e-17
ref|XP_021654563.1| uncharacterized protein LOC110645648 [Hevea ... 96 6e-17
ref|XP_009791116.1| PREDICTED: uncharacterized protein LOC104238... 97 7e-17
gb|KYP73844.1| Retrovirus-related Pol polyprotein from transposo... 87 1e-16
gb|PNX58963.1| retrotransposon-related protein, partial [Trifoli... 92 1e-16
gb|ADN34141.1| ty3-gypsy retrotransposon protein [Cucumis melo s... 97 1e-16
gb|ADU56212.1| gag-pol polyprotein [Solanum lycopersicum] 96 1e-16
>ref|XP_017604286.1| PREDICTED: RNA-directed DNA polymerase homolog [Gossypium arboreum]
Length = 164
Score = 97.4 bits (241), Expect = 9e-20
Identities = 52/109 (47%), Positives = 73/109 (66%), Gaps = 6/109 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKIEE-Y 901
KT F RYGHYEF+++ AP AF+ LMN++F+ Y DRF +VFI++IL+YSKIEE +
Sbjct: 56 KTAFRTRYGHYEFLVMPFGLTNAPAAFMDLMNRVFQPYLDRFVVVFIDDILVYSKIEEKH 115
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEM 754
E+H RIVL L EK L+A + SFLGH +S +G+ D +++
Sbjct: 116 EEHLRIVLQVLREKELYAKFSKCEFWLQEVSFLGHVVSAEGIRVDPRKI 164
>ref|XP_017701988.1| PREDICTED: RNA-directed DNA polymerase homolog [Phoenix dactylifera]
Length = 205
Score = 94.7 bits (234), Expect = 2e-18
Identities = 51/108 (47%), Positives = 69/108 (63%), Gaps = 6/108 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKI-EEY 901
KT F RYGHYEF+++ AP F+ LMN++F+ Y D+F +VFIN+IL+YSK +E+
Sbjct: 45 KTAFRTRYGHYEFLVMPFGLTNAPATFMDLMNRVFKPYLDQFVVVFINDILIYSKSPQEH 104
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKE 757
E+H RIVL TL E L+ +FLGH ISKDG+ D K+
Sbjct: 105 EEHLRIVLQTLRENKLYGKLQKCEFWLNSITFLGHVISKDGISVDPKK 152
>ref|XP_015075579.1| PREDICTED: RNA-directed DNA polymerase homolog, partial [Solanum
pennellii]
Length = 191
Score = 93.6 bits (231), Expect = 4e-18
Identities = 52/110 (47%), Positives = 71/110 (64%), Gaps = 6/110 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKIE-EY 901
KT F RYGHYEFV++ AP AF+ LMN IF+ Y D F +VFI++IL+YSK + E+
Sbjct: 56 KTAFRTRYGHYEFVVMSFGLTNAPAAFMSLMNGIFKPYLDLFVIVFIDDILVYSKSKKEH 115
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK 751
E+H R+VL L EK L+A + SFLGH +SKDG+ D +++
Sbjct: 116 EEHLRMVLEMLREKKLYAKFSKCEFWLDAVSFLGHVVSKDGVMVDPSKIE 165
>ref|XP_019066470.1| PREDICTED: uncharacterized protein LOC109118867 [Solanum
lycopersicum]
Length = 189
Score = 93.2 bits (230), Expect = 5e-18
Identities = 50/110 (45%), Positives = 72/110 (65%), Gaps = 6/110 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKIE-EY 901
KT F RYGHYEFV++ AP +F+ LMN+IF+ Y D + +VFI++IL+YSK + E+
Sbjct: 56 KTAFRTRYGHYEFVVMSFGLTNAPASFMSLMNRIFKPYLDLYVIVFIDDILVYSKSKKEH 115
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK 751
E+H R+VL L EK L+A + SFLGH +SKDG+ D +++
Sbjct: 116 EEHLRMVLEMLREKKLYAKFSKCEFWLDAVSFLGHVVSKDGVKVDPSKIE 165
>ref|XP_012441962.1| PREDICTED: uncharacterized protein LOC105766968 [Gossypium raimondii]
Length = 1030
Score = 100 bits (249), Expect = 6e-18
Identities = 58/140 (41%), Positives = 87/140 (62%), Gaps = 6/140 (4%)
Frame = -2
Query: 1080 ERIYAYKTTFLARYGHYEF-VMLC----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYS 916
+ I YK TF RYGHYEF V LC AP F+ LMN++F Y DRF +VFI+NIL+YS
Sbjct: 549 KEINVYKKTFRTRYGHYEFLVKLCGLMNAPATFMNLMNRVFLPYLDRFVVVFIDNILVYS 608
Query: 915 KIE-EYEKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK*LLI 739
K E E+++H R+VL L EK L+A + + +FLGH +S +G+ D ++++ ++
Sbjct: 609 KTEDEHDEHFRMVLQILREKQLYAKFSKYEFWLRKVTFLGHVVSNEGIRVDPRKIE-AVL 667
Query: 738 EIR*PVTSLNLKEDLSHTEH 679
+ + P T ++ L TE+
Sbjct: 668 DWKQPKTVSKIRSFLGLTEY 687
>ref|XP_021607608.1| uncharacterized protein LOC110611529 [Manihot esculenta]
Length = 796
Score = 100 bits (248), Expect = 6e-18
Identities = 54/116 (46%), Positives = 77/116 (66%), Gaps = 6/116 (5%)
Frame = -2
Query: 1071 YAYKTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKI- 910
Y KT F RYGHYEF+++ AP AF+ LMN+IF + D+F +VFIN+IL+YSKI
Sbjct: 421 YIPKTAFRTRYGHYEFLVMPFGLTNAPAAFMDLMNRIFHPFLDQFVVVFINDILVYSKIK 480
Query: 909 EEYEKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK*LL 742
EE+++H RIVL TL EK L+ + + SFLGH +S +G+ D K+++ +L
Sbjct: 481 EEHDRHLRIVLQTLREKQLYVKFSKCKFWLNEISFLGHVVSAEGIRVDPKKIETIL 536
>ref|XP_016675550.1| PREDICTED: RNA-directed DNA polymerase homolog, partial [Gossypium
hirsutum]
Length = 187
Score = 92.8 bits (229), Expect = 7e-18
Identities = 46/117 (39%), Positives = 75/117 (64%), Gaps = 6/117 (5%)
Frame = -2
Query: 1083 YERIYAYKTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLY 919
++ + YKT F RYGHYEF+++ AP F+ LMN++F+ Y DRF +VFI+ IL+Y
Sbjct: 49 FKEVDIYKTAFRTRYGHYEFLVMPFGLTNAPATFMDLMNRVFQPYLDRFVVVFIDEILVY 108
Query: 918 SKIEE-YEKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK 751
S+ EE +++H R+VL L E+ L+A + +FLGH +S +G+ D ++++
Sbjct: 109 SETEEKHDEHLRVVLQVLRERQLYAKFSKCEFWLKEVTFLGHVVSAEGIKVDPRKIE 165
>ref|XP_016675304.1| PREDICTED: RNA-directed DNA polymerase homolog [Gossypium hirsutum]
Length = 223
Score = 92.8 bits (229), Expect = 2e-17
Identities = 46/117 (39%), Positives = 75/117 (64%), Gaps = 6/117 (5%)
Frame = -2
Query: 1083 YERIYAYKTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLY 919
++ + YKT F RYGHYEF+++ AP F+ LMN++F+ Y DRF +VFI+ IL+Y
Sbjct: 49 FKEVDIYKTAFRTRYGHYEFLVMPFGLTNAPATFMDLMNRVFQPYLDRFVVVFIDEILVY 108
Query: 918 SKIEE-YEKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK 751
S+ EE +++H R+VL L E+ L+A + +FLGH +S +G+ D ++++
Sbjct: 109 SETEEKHDEHLRVVLQVLRERQLYAKFSKCEFWLKEVTFLGHVVSAEGIKVDPRKIE 165
>ref|XP_016556474.1| PREDICTED: RNA-directed DNA polymerase homolog [Capsicum annuum]
Length = 238
Score = 92.8 bits (229), Expect = 2e-17
Identities = 49/109 (44%), Positives = 72/109 (66%), Gaps = 5/109 (4%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKIE-EYE 898
KT F RYGH+EF+M+ AP AF+ LMN++F+ Y D + +VFI++IL+YS+ E E
Sbjct: 58 KTAFRTRYGHFEFLMIFGLTNAPAAFMDLMNRVFKPYLDHYVIVFIDDILVYSRREAEQG 117
Query: 897 KHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK 751
+H R+VL TL E+ L+A + SFLGH +S+DG+ D K++K
Sbjct: 118 QHLRVVLQTLREQKLYAKFSKREFWLGTVSFLGHVVSRDGIHVDPKKIK 166
>ref|XP_016752415.1| PREDICTED: RNA-directed DNA polymerase homolog [Gossypium hirsutum]
Length = 150
Score = 90.1 bits (222), Expect = 2e-17
Identities = 48/103 (46%), Positives = 67/103 (65%), Gaps = 6/103 (5%)
Frame = -2
Query: 1065 YKTTFLARYGHYEFV-----MLCAPVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKIE-E 904
YKT F RYGHYEF ++ AP AF+ LMN++F+ Y D+F VFI++IL+YSK E E
Sbjct: 48 YKTAFKTRYGHYEFPVMPFNLMNAPTAFMDLMNRVFQPYLDQFIWVFIDDILIYSKTESE 107
Query: 903 YEKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGM 775
+++H RIVL L EK L+A + +FLGH +S +G+
Sbjct: 108 HDEHLRIVLHILREKQLYAKFSKCEFLLQEVTFLGHVVSVEGI 150
>gb|EOY20476.1| Uncharacterized protein TCM_046344 [Theobroma cacao]
Length = 740
Score = 98.2 bits (243), Expect = 2e-17
Identities = 52/110 (47%), Positives = 73/110 (66%), Gaps = 6/110 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKI-EEY 901
KT F RYGHYEF++ AP AF+ LMN++F+ Y D+F +VFIN+IL+YSK EE+
Sbjct: 454 KTAFRTRYGHYEFLVTSFGLTNAPAAFMDLMNRVFKPYLDKFVVVFINDILIYSKSREEH 513
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK 751
E+H +IVL TL E L+A + +FLGH +SKDG+ D K+++
Sbjct: 514 EQHLKIVLQTLREYRLYAKFSKCEFWLESVAFLGHVVSKDGVQVDPKKVE 563
>gb|OMO91869.1| reverse transcriptase [Corchorus capsularis]
Length = 1401
Score = 98.6 bits (244), Expect = 3e-17
Identities = 54/110 (49%), Positives = 73/110 (66%), Gaps = 6/110 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKI-EEY 901
KTTF RY HYEF+++ AP AF+ LMN+IF+ Y D+F +VFI++IL+YSK EE+
Sbjct: 642 KTTFRTRYAHYEFLVMPFGLTNAPAAFMDLMNRIFKQYLDQFVVVFIDDILIYSKSDEEH 701
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK 751
E H RIVL TL +K L A + +FLGH ISKDG+ D K+++
Sbjct: 702 ENHLRIVLQTLRDKQLFAKFSKCEFWMRSVAFLGHVISKDGISVDPKKIE 751
>gb|PPY92755.1| hypothetical protein C5P31_25760, partial [Escherichia coli]
Length = 355
Score = 94.4 bits (233), Expect = 5e-17
Identities = 52/110 (47%), Positives = 73/110 (66%), Gaps = 6/110 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKI-EEY 901
KT F RYGHYEF+++ AP AF+ LMN+IF + DRF +VFI++IL+YSK EE+
Sbjct: 8 KTAFRTRYGHYEFLVMPFGLTNAPAAFMDLMNRIFHPHLDRFVVVFIDDILVYSKTSEEH 67
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK 751
++H RIVL TL EK L+A + SFLGH +S +G+ D K+++
Sbjct: 68 DEHLRIVLQTLREKKLYAKLSKCDFWMSEISFLGHIVSTEGIRVDPKKIE 117
>ref|XP_016743303.1| PREDICTED: RNA-directed DNA polymerase homolog, partial [Gossypium
hirsutum]
Length = 224
Score = 91.3 bits (225), Expect = 5e-17
Identities = 50/113 (44%), Positives = 71/113 (62%), Gaps = 6/113 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKIE-EY 901
KT F RYGHYEF+++ AP F+ LMN+IFR Y DRF +VFI++IL+YS+ E E+
Sbjct: 55 KTAFRTRYGHYEFLVMPFGLTNAPAVFMDLMNRIFRPYLDRFVVVFIDDILVYSRDEIEH 114
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK*LL 742
KH R+VL L +K L+A + + SFLGH +S G+ D ++ +L
Sbjct: 115 AKHLRLVLQVLRDKKLYAKFSKCEFWLRKVSFLGHVVSASGIRVDPNKISAIL 167
>ref|XP_021654563.1| uncharacterized protein LOC110645648 [Hevea brasiliensis]
Length = 508
Score = 95.9 bits (237), Expect = 6e-17
Identities = 50/110 (45%), Positives = 75/110 (68%), Gaps = 3/110 (2%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLCA--PVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKI-EEYEKH 892
KT+F RYGHYEF+++ A P F+ LMN++F+ Y D F +VFI++IL+YSK EE+++H
Sbjct: 398 KTSFQTRYGHYEFLIMLANAPATFMDLMNRVFQPYLDSFVVVFIDDILVYSKSKEEHDQH 457
Query: 891 SRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK*LL 742
RI+L TL EK LHA + L + +FLGH +S +G+ ++K +L
Sbjct: 458 LRIILQTLREKKLHAKFSKCELWLKQVAFLGHIVSTEGIRVHPSKIKAIL 507
>ref|XP_009791116.1| PREDICTED: uncharacterized protein LOC104238456 [Nicotiana
sylvestris]
Length = 728
Score = 96.7 bits (239), Expect = 7e-17
Identities = 49/110 (44%), Positives = 74/110 (67%), Gaps = 6/110 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKIE-EY 901
KT F RYGH+EF+++ AP AF+ LMN++F+ + D F +VFIN+IL+YS+ E E+
Sbjct: 483 KTAFRTRYGHFEFLVMSFGLTNAPAAFMDLMNRVFKPFLDHFVIVFINDILVYSRSEAEH 542
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK 751
E+H R+VL TL E+ L+A + SFLGH +S+DG+ D K+++
Sbjct: 543 EQHLRVVLQTLRERTLYAKFSKCEFWLDTVSFLGHVVSRDGIQVDPKKIE 592
>gb|KYP73844.1| Retrovirus-related Pol polyprotein from transposon 17.6, partial
[Cajanus cajan]
Length = 114
Score = 87.0 bits (214), Expect = 1e-16
Identities = 48/114 (42%), Positives = 70/114 (61%), Gaps = 6/114 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKI-EEY 901
KT F RYGHYE+ ++ AP F+ MN+IFR Y D F +VFI++IL+YS+ EE+
Sbjct: 1 KTAFRTRYGHYEYQVMPFGVTNAPAVFMDYMNRIFRPYLDHFVVVFIDDILIYSRTHEEH 60
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK*LLI 739
E+H + VL L++K L+A FLGH ISKDG+ D +++ +L+
Sbjct: 61 EEHLQTVLQILKDKQLYAKLSKCEFWLEEVKFLGHVISKDGVSVDPSKVEAVLL 114
>gb|PNX58963.1| retrotransposon-related protein, partial [Trifolium pratense]
Length = 287
Score = 92.0 bits (227), Expect = 1e-16
Identities = 48/110 (43%), Positives = 73/110 (66%), Gaps = 6/110 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKI-EEY 901
KT F RYGHYEF+ + AP AF+ LMN+IF+ + D+F +VFI++IL+YSK EE+
Sbjct: 139 KTAFRTRYGHYEFLFMSFRLTNAPTAFMDLMNRIFKPFLDQFVIVFIDDILIYSKSKEEH 198
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK 751
E+H R+VL TL+E L+A + +FLG+ +SK+G+ D +++
Sbjct: 199 ERHLRLVLQTLKENKLYAKFSKCEFWLDNVTFLGYVVSKNGISVDPSKVE 248
>gb|ADN34141.1| ty3-gypsy retrotransposon protein [Cucumis melo subsp. melo]
Length = 1359
Score = 96.7 bits (239), Expect = 1e-16
Identities = 50/105 (47%), Positives = 72/105 (68%), Gaps = 6/105 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKIE-EY 901
KT F +RYGHYEF+++ AP F+ LMN++FR + D F +VFIN+IL+YSKIE E+
Sbjct: 560 KTAFHSRYGHYEFIVMSFALTNAPSVFMDLMNRVFREFLDTFVIVFINDILIYSKIEAEH 619
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPAD 766
E+H R+VL TL++ L+A ++ + SFLGH +SK G+ D
Sbjct: 620 EEHLRMVLQTLQDNKLYAKFLKCEFWLKQVSFLGHVVSKAGVSVD 664
>gb|ADU56212.1| gag-pol polyprotein [Solanum lycopersicum]
Length = 624
Score = 95.5 bits (236), Expect = 1e-16
Identities = 53/110 (48%), Positives = 71/110 (64%), Gaps = 6/110 (5%)
Frame = -2
Query: 1062 KTTFLARYGHYEFVMLC-----APVAFIGLMNKIFR*Y*DRFDMVFINNILLYSKI-EEY 901
KT F RYGHYEFV++ AP AF+ LMN IF+ Y D F +VFI++IL+YSK EE+
Sbjct: 148 KTAFRTRYGHYEFVVMSFGLTNAPAAFMSLMNGIFKPYLDLFVIVFIDDILIYSKSKEEH 207
Query: 900 EKHSRIVLWTLEEKPLHASWVNMRLCWTRASFLGHPISKDGMPADQKEMK 751
E+H R+VL L EK L+A + SFLGH +SKDG+ D +++
Sbjct: 208 EEHLRMVLEMLREKKLYAKFSKCEFWLDTVSFLGHVVSKDGVMVDPSKIE 257