BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon27_contig00007100
         (2559 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020273972.1| isoamylase 2, chloroplastic [Asparagus offic...  1163   0.0  
ref|XP_008799133.1| PREDICTED: isoamylase 2, chloroplastic [Phoe...  1117   0.0  
ref|XP_010938750.1| PREDICTED: isoamylase 2, chloroplastic [Elae...  1080   0.0  
gb|PKA66852.1| Isoamylase 2, chloroplastic [Apostasia shenzhenica]   1026   0.0  
ref|XP_020700621.1| isoamylase 2, chloroplastic [Dendrobium cate...  1025   0.0  
ref|XP_020087102.1| isoamylase 2, chloroplastic [Ananas comosus]...  1018   0.0  
ref|XP_010278620.1| PREDICTED: isoamylase 2, chloroplastic [Nelu...  1014   0.0  
gb|OAY83378.1| Isoamylase 2, chloroplastic [Ananas comosus]          1007   0.0  
ref|XP_018683242.1| PREDICTED: isoamylase 2, chloroplastic-like ...   983   0.0  
gb|PIA39269.1| hypothetical protein AQUCO_02600010v1 [Aquilegia ...   968   0.0  
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   964   0.0  
ref|XP_020592870.1| isoamylase 2, chloroplastic [Phalaenopsis eq...   959   0.0  
ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber] ...   954   0.0  
ref|XP_011624242.1| isoamylase 2, chloroplastic isoform X2 [Ambo...   951   0.0  
gb|ERN08315.1| hypothetical protein AMTR_s00156p00067780 [Ambore...   951   0.0  
gb|AOQ26242.1| ISA2 [Actinidia deliciosa]                             942   0.0  
ref|XP_018858427.1| PREDICTED: isoamylase 2, chloroplastic [Jugl...   939   0.0  
gb|OMO77794.1| hypothetical protein CCACVL1_14832 [Corchorus cap...   938   0.0  
ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]...   936   0.0  
ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium]        935   0.0  

>ref|XP_020273972.1| isoamylase 2, chloroplastic [Asparagus officinalis]
 ref|XP_020273973.1| isoamylase 2, chloroplastic [Asparagus officinalis]
 gb|ONK62840.1| uncharacterized protein A4U43_C07F8670 [Asparagus officinalis]
          Length = 855

 Score = 1163 bits (3009), Expect = 0.0
 Identities = 578/825 (70%), Positives = 652/825 (79%), Gaps = 9/825 (1%)
 Frame = +3

Query: 3    KLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVV 182
            K +N K+    HNS EQI PG  LNYNAE  QR  SYLFRTE+GGLVKVLVG NNMKY V
Sbjct: 49   KPQNAKVFLKPHNSIEQIHPGSDLNYNAETGQRATSYLFRTEIGGLVKVLVGFNNMKYAV 108

Query: 183  NIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQ 362
             I+VSSLP   N  DLILSWG+ RSDS+ L AP                     +TP  Q
Sbjct: 109  RIDVSSLPTRTN--DLILSWGVHRSDSNQLDAP------------------GCTKTPLVQ 148

Query: 363  NSLGTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPL 542
            N  G  ++TLEF S Q+P                +SGI+THR TNFC+PVG+S+G PMPL
Sbjct: 149  NLKGRFSVTLEFSSTQIPFYLSFLLHSSSDHPRCESGIRTHRMTNFCLPVGMSAGHPMPL 208

Query: 543  GFSKSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGN 722
            G S+ E GLVNFALFSR AESV L L+++  N+P L++ELDPY+NRTGDIWH+SME+VG+
Sbjct: 209  GVSRCEGGLVNFALFSRNAESVVLRLFDNNVNEPCLELELDPYVNRTGDIWHLSMENVGS 268

Query: 723  YVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGES----VYLGYLGDEPTF 890
            Y SYGYRCKGE   D GSRFHA  VLLDPYAKLI N  P++GES      LG L +EP F
Sbjct: 269  YTSYGYRCKGETGSDKGSRFHARQVLLDPYAKLIANVSPEKGESRSLVKCLGSLAEEPIF 328

Query: 891  DWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVL 1070
            DWSGDVHPQLPMEKLVVYRLNVG+FTKD+SSELPENVAGTFAGVIEKL H KSLG+NA+L
Sbjct: 329  DWSGDVHPQLPMEKLVVYRLNVGNFTKDESSELPENVAGTFAGVIEKLPHLKSLGINAIL 388

Query: 1071 LEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFT 1250
            LEPIF +DEK GPYFPYHFFSPMNSYG   D +ST+ SMK+MVK LHAHGIEVLLEVVFT
Sbjct: 389  LEPIFTFDEKEGPYFPYHFFSPMNSYGYKSDSISTINSMKDMVKTLHAHGIEVLLEVVFT 448

Query: 1251 HTGEGG-TACQTISFRGIDNSSYYITEGGMGSGM----NCNNPIVQQLILDSLHYWLTEF 1415
            HTGEGG ++ QT SFRGID SSYYI EG M  G+    N N+P+VQQLIL+SLHYWLTEF
Sbjct: 449  HTGEGGDSSSQTKSFRGIDYSSYYIVEGDMTQGIYNAVNSNDPVVQQLILESLHYWLTEF 508

Query: 1416 HVDGFCFINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFP 1595
            H+DGFCFINSSYLL+GSNG+ LS PPLVEAI+FDPLLSK+KIIADCW PVD S+ DIHFP
Sbjct: 509  HIDGFCFINSSYLLRGSNGEYLSHPPLVEAISFDPLLSKSKIIADCWSPVDGSYLDIHFP 568

Query: 1596 HWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLP 1775
            HW+RWAE+N RFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGP FSFNYVTKNFGLP
Sbjct: 569  HWKRWAEMNTRFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPAFSFNYVTKNFGLP 628

Query: 1776 LVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDE 1955
            LVDLVSFS +ELASELSWNCG+EG TN   VLE+RL+QIRNFLF+LFISLG+PV NMGDE
Sbjct: 629  LVDLVSFSRDELASELSWNCGKEGPTNNNSVLEIRLRQIRNFLFLLFISLGVPVLNMGDE 688

Query: 1956 CGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNID 2135
            CGFSTGGL +Y  R PIDWN  K+V+GKQITQFI FLSSLR RR+D+FQ RDFLKV+NI 
Sbjct: 689  CGFSTGGLSSYGDRHPIDWNVTKSVFGKQITQFITFLSSLRNRRNDIFQRRDFLKVENIV 748

Query: 2136 WHGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEG 2315
            W GSN S PKWEDPSCKFLA+T+RA+K+  + +S KGDLF+CFN  D PE  ILP L EG
Sbjct: 749  WQGSNLSQPKWEDPSCKFLAVTIRADKDDNMSTSYKGDLFICFNADDFPEIAILPPLLEG 808

Query: 2316 YAWVCLVDTALPYPGFFSMDSNFCDGLSTYELKPHSCTLFEAGMS 2450
            Y W  LVDT+LP+PGFFS +S F + L  YELKPHSC LFE   S
Sbjct: 809  YEWFRLVDTSLPFPGFFSGESVFTEALLGYELKPHSCVLFEVKKS 853


>ref|XP_008799133.1| PREDICTED: isoamylase 2, chloroplastic [Phoenix dactylifera]
          Length = 853

 Score = 1117 bits (2889), Expect = 0.0
 Identities = 547/819 (66%), Positives = 650/819 (79%), Gaps = 13/819 (1%)
 Frame = +3

Query: 33   SHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLY 212
            S +S EQ+ P LG++ + ++LQ+ FSY+FRTE+GGLVKVLVG  +M+Y V +EVSSLP  
Sbjct: 57   SQSSVEQLAPDLGVSDSTQELQKPFSYMFRTEIGGLVKVLVGTKSMRYAVRVEVSSLPRA 116

Query: 213  LNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITL 392
            + E  L+++WGMFRSDSS L A DSQ +                   F +NSLG + + L
Sbjct: 117  VGEDGLVMNWGMFRSDSSQLLAQDSQVA-------------------FVRNSLGGYMVEL 157

Query: 393  EFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSEDGLV 572
            EFDSA++P                 S I+THRKT FCVPVG+  G PMPLG S S+DG V
Sbjct: 158  EFDSARIPFYLSFLLSSPPSV----SEIRTHRKTKFCVPVGLGPGWPMPLGVSISDDGAV 213

Query: 573  NFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKG 752
            NF+LFSR AE V LCL++    +PSL+++LDPY+NRTGDIWHVSMES+GN+VSYGYRCKG
Sbjct: 214  NFSLFSRNAEGVVLCLFDGKTREPSLEMDLDPYVNRTGDIWHVSMESMGNFVSYGYRCKG 273

Query: 753  EITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVYL----GYLGDEPTFDWSGDVHPQL 920
               WD   RFHA HVLLDPYAK++ +F+PD+GESV L    G LG E +FDWSGDVHP+L
Sbjct: 274  SGLWDKEDRFHAHHVLLDPYAKVLGDFVPDRGESVSLAKCLGSLGFEHSFDWSGDVHPRL 333

Query: 921  PMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFPYDEK 1100
            PMEKLVVYRLNVG FTK+K+S LPENV+GTFAG+IEK++HF++LGVNAVLLEPIF +DE 
Sbjct: 334  PMEKLVVYRLNVGQFTKNKTSGLPENVSGTFAGLIEKVEHFRTLGVNAVLLEPIFSFDEH 393

Query: 1101 RGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGE-GGTAC 1277
            +GPYFPYHFFSPM+SYG   DG+S + SMKEMVK LHAHGIEVLLEV FT TGE G  AC
Sbjct: 394  KGPYFPYHFFSPMHSYGHASDGVSAINSMKEMVKMLHAHGIEVLLEVAFTQTGEEGDAAC 453

Query: 1278 QTISFRGIDNSSYYITEGGMGSG----MNCNNPIVQQLILDSLHYWLTEFHVDGFCFINS 1445
            QTISFRGIDNSSYYI +G +GSG    + C+NP+VQQLILDSL +W+ EFHVDGFCFINS
Sbjct: 454  QTISFRGIDNSSYYIVDGDLGSGAYNVLKCSNPVVQQLILDSLRHWVVEFHVDGFCFINS 513

Query: 1446 SYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRWAEINA 1625
            S+L +G NGD LSRPPLVEAIA DP+LSKTKIIADCW P+D S+ +I FPHW+RWAE+N 
Sbjct: 514  SFLARGLNGDYLSRPPLVEAIAVDPILSKTKIIADCWSPLDNSYMEIQFPHWKRWAEMNT 573

Query: 1626 RFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVSFSSN 1805
            RFC DVRNFLRGEGLL DLATRLCGSGDLFS SRGP FSFNY+TKNFGL LVDLVSFS+ 
Sbjct: 574  RFCSDVRNFLRGEGLLRDLATRLCGSGDLFS-SRGPAFSFNYITKNFGLTLVDLVSFSNG 632

Query: 1806 ELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTGGLFA 1985
            +LASELSWNCGEEG+TN   VL++RLKQIRNFLF+LF+SLG+PV NMGDECG+STGG  +
Sbjct: 633  DLASELSWNCGEEGSTNNNTVLDIRLKQIRNFLFVLFVSLGVPVLNMGDECGYSTGGSPS 692

Query: 1986 YDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQSDPK 2165
            Y GRQPI WNGL+TV+G+QITQFIAF+SSLR+RR D+FQ R+FL+++NIDWHGS+QS P 
Sbjct: 693  YIGRQPIGWNGLQTVFGRQITQFIAFMSSLRMRRGDIFQRRNFLELENIDWHGSSQSRPN 752

Query: 2166 WEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCLVDTA 2345
            W  PSCKFLAMTLRAE + KL +SD GDLF+ FN S  PES +LP+  EG  W+ LVDT+
Sbjct: 753  WGKPSCKFLAMTLRAEMDDKLSNSDDGDLFISFNASAHPESTVLPEQSEGVVWLRLVDTS 812

Query: 2346 LPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEAGMS 2450
            LP+PGFFS DS    +   GLS+YELKPHSC LFEA  S
Sbjct: 813  LPFPGFFSSDSDPSVHQAAGLSSYELKPHSCVLFEAKRS 851


>ref|XP_010938750.1| PREDICTED: isoamylase 2, chloroplastic [Elaeis guineensis]
          Length = 851

 Score = 1080 bits (2793), Expect = 0.0
 Identities = 532/823 (64%), Positives = 632/823 (76%), Gaps = 13/823 (1%)
 Frame = +3

Query: 21   ISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSS 200
            +S++S    EQ+ P L ++ + ++L++ FSY+FR E GGL KVLVG  +MKY V +EVS 
Sbjct: 52   MSQSSVEHVEQLAPDLEVSDSTQELEKPFSYIFRAETGGLAKVLVGVKDMKYAVRVEVSF 111

Query: 201  LPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTH 380
            LP  + E  L+++WGMFRSDS  L   DSQ +                   F QNSLG +
Sbjct: 112  LPRAVGEDGLVMNWGMFRSDSKQLLVQDSQVA-------------------FVQNSLGGY 152

Query: 381  AITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSE 560
             + LEFDS ++P                 S I+TH+KT FCVPVGI  G PMPLG S S+
Sbjct: 153  MVELEFDSVEIPFYLSFLLSSPSV-----SEIRTHKKTKFCVPVGIGPGCPMPLGVSISD 207

Query: 561  DGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGY 740
            DG +NF++FSR AE V L L++    +PSL+I+LDPY+NRTGDIWHVSMES+ NY  YGY
Sbjct: 208  DGAINFSVFSRNAEGVVLYLFDGRTREPSLEIDLDPYVNRTGDIWHVSMESIENYAGYGY 267

Query: 741  RCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVYL----GYLGDEPTFDWSGDV 908
            RCKG   WD G +FHA  +LLDPYAK++ +F+ DQGES+ L    G L  E  FDW+GDV
Sbjct: 268  RCKGLALWDKGDKFHAHDILLDPYAKVLGDFVLDQGESMSLAKCLGSLDIEHGFDWTGDV 327

Query: 909  HPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFP 1088
            HP LPMEKLVVYRLNVG FTK K+S LPENVAGTFAG+IEK++H KSLGVNAVLLEPIF 
Sbjct: 328  HPCLPMEKLVVYRLNVGQFTKHKTSRLPENVAGTFAGLIEKVEHLKSLGVNAVLLEPIFS 387

Query: 1089 YDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEG- 1265
            +DE++GPYFPYHFFSPMNSYG   DG+S + SMK+MVK LHAHGIEVLLEV FT TGEG 
Sbjct: 388  FDEQKGPYFPYHFFSPMNSYGYASDGVSAIDSMKKMVKMLHAHGIEVLLEVAFTQTGEGE 447

Query: 1266 GTACQTISFRGIDNSSYYITEGGMGSG----MNCNNPIVQQLILDSLHYWLTEFHVDGFC 1433
              AC+TISFRGIDNSSYYI +  +G G    + C+NP+VQQLILDSL +W+ EFHVDGFC
Sbjct: 448  DAACRTISFRGIDNSSYYIVDRDLGYGAHNALKCSNPVVQQLILDSLRHWVIEFHVDGFC 507

Query: 1434 FINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRWA 1613
            FINSS+L QG NGD L RPPLVEAIAFDP+LSKTKIIADCW P D S+ +I FPHW+RWA
Sbjct: 508  FINSSFLAQGLNGDYLYRPPLVEAIAFDPILSKTKIIADCWSPPDNSYMEIRFPHWKRWA 567

Query: 1614 EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 1793
            E+N RFC DVRNFLRGEGLLS+LATRLCGSGDLFS SRGP FSFNY+TKNFGL LVDLVS
Sbjct: 568  EMNTRFCSDVRNFLRGEGLLSNLATRLCGSGDLFS-SRGPAFSFNYITKNFGLSLVDLVS 626

Query: 1794 FSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTG 1973
            +S  +LASELSWNCGEEG TN   VL++RLKQIRNFLF+LF+SLG+PV NMGDECG+STG
Sbjct: 627  YSDGDLASELSWNCGEEGPTNNNTVLDLRLKQIRNFLFVLFVSLGVPVLNMGDECGYSTG 686

Query: 1974 GLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQ 2153
            G  +Y  R+PI WNGLKTV+G+QITQFIAFLSSLR+RR D+FQ RDFLKV+NIDWHGS+Q
Sbjct: 687  GTPSYIDRKPIGWNGLKTVFGRQITQFIAFLSSLRVRRGDIFQRRDFLKVENIDWHGSSQ 746

Query: 2154 SDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCL 2333
            S P W +PSCKFLA+TL+AE + KL +S+ GDLF+ FN SDL ES +LP+  EG  W+ L
Sbjct: 747  SQPNWREPSCKFLAVTLKAEMDDKLSNSNDGDLFISFNASDLLESAVLPEQSEGIVWLRL 806

Query: 2334 VDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEAGMS 2450
            VDTALP+PGFFS DS    +   GLS+Y+LKPHSC LFEA  S
Sbjct: 807  VDTALPFPGFFSSDSDPDVHQAAGLSSYDLKPHSCVLFEAKRS 849


>gb|PKA66852.1| Isoamylase 2, chloroplastic [Apostasia shenzhenica]
          Length = 881

 Score = 1026 bits (2653), Expect = 0.0
 Identities = 517/821 (62%), Positives = 608/821 (74%), Gaps = 13/821 (1%)
 Frame = +3

Query: 18   KISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVS 197
            ++  A+ +S + +QP   + +N E  Q+ +SYLFRTE  G+VKVLV  N+MKY V IE+ 
Sbjct: 71   RVLAAAPSSQKDVQPVSDVTFNFEDQQKEYSYLFRTEFDGMVKVLVRINDMKYTVYIELQ 130

Query: 198  SLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGT 377
            SLP  + +HDLIL W  FRSDS +     SQ S L             ++TP T+ S G 
Sbjct: 131  SLPKPIIDHDLILCWHTFRSDSENSSGLGSQISALSADHF--------MKTPLTRGSSGM 182

Query: 378  HAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKS 557
            H   LEF   Q+P              A  S IKT RKT F VPVG+ SG  +PLG S  
Sbjct: 183  HTAVLEFSFQQIPFYLSFLLQVDG---AAKSEIKTERKTYFRVPVGLGSGHSLPLGISLF 239

Query: 558  EDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYG 737
            +DG  NF+LFSR AESV LCLY+   N+PS +IELDPY+NRTGDIWHV +  + NY  YG
Sbjct: 240  DDGSANFSLFSRNAESVVLCLYDGKTNEPSFEIELDPYVNRTGDIWHVLLYDLSNYTRYG 299

Query: 738  YRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVYL----GYLGDEPTFDWSGD 905
            YRCKG+I WD GSRFHA HVLLDPYAK I  F PD+G SV L    G L   P FDW+GD
Sbjct: 300  YRCKGKIFWDTGSRFHARHVLLDPYAKTIGEFCPDEGGSVSLSKCLGCLRKMPKFDWNGD 359

Query: 906  VHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIF 1085
             HPQ+ MEKLVVYRLNVG FTK+KSS LPENVAGTFAGV +K+QH  SLGVNAVLLEPIF
Sbjct: 360  THPQISMEKLVVYRLNVGRFTKEKSSGLPENVAGTFAGVTKKIQHLTSLGVNAVLLEPIF 419

Query: 1086 PYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEG 1265
            P+D+ R PYFPYHFFSPMN YG   +  S V  MKEMVK LH +GIEVL+EVVFTHT EG
Sbjct: 420  PFDKNRNPYFPYHFFSPMNEYGDVDEFNSAVNLMKEMVKTLHTNGIEVLMEVVFTHTCEG 479

Query: 1266 G-TACQTISFRGIDNSSYYITE----GGMGSGMNCNNPIVQQLILDSLHYWLTEFHVDGF 1430
              T  + ISFRGIDNSSYYI E     G+ S +NCN+  VQQ+ILDSLHYWLTEFHVDGF
Sbjct: 480  NDTKSRIISFRGIDNSSYYIVEPNGASGINSMLNCNSAAVQQIILDSLHYWLTEFHVDGF 539

Query: 1431 CFINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRW 1610
            CFINSS LLQGSN D L R PL+EAIAFDPLL+KTKII+DCW P+D S  D+ FPHWRRW
Sbjct: 540  CFINSSSLLQGSNSDDLPRSPLIEAIAFDPLLTKTKIISDCWSPIDESCKDVVFPHWRRW 599

Query: 1611 AEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLV 1790
             E+N +F RDVRNFL+GEGLLS+ ATRLCGSGD+F  SRGP++SFNYVTKNFGL LVDLV
Sbjct: 600  GEMNPKFSRDVRNFLKGEGLLSEFATRLCGSGDIFLHSRGPSYSFNYVTKNFGLTLVDLV 659

Query: 1791 SFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFST 1970
            SFS  +LASELSWNCG+EG T+K +VLE+RL+QIRNFLFILFISLG+PV NMGDECG+S 
Sbjct: 660  SFSGADLASELSWNCGDEGPTDKSIVLEIRLRQIRNFLFILFISLGVPVLNMGDECGYSA 719

Query: 1971 GGLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSN 2150
            GG  +YD R P+DW+ L+T +G+QIT FI+FL+S R RRSD+FQ ++FL+++NI WHGSN
Sbjct: 720  GGSPSYDERMPLDWSNLRTEFGRQITSFISFLTSFRDRRSDVFQRKEFLELENIAWHGSN 779

Query: 2151 QSDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVC 2330
            QS  KWEDPSCKFLAM L+AE++     S  G+LF+ FN S   ES  LP LPEG AW  
Sbjct: 780  QSPLKWEDPSCKFLAMDLKAEEDAGSSDSGNGNLFISFNASADIESANLPGLPEGQAWFR 839

Query: 2331 LVDTALPYPGFFS--MDSNFC--DGLSTYELKPHSCTLFEA 2441
            LVDT+LPYPGFFS   D++ C  +GLS+YELKPHSC LFEA
Sbjct: 840  LVDTSLPYPGFFSSDFDADVCKFEGLSSYELKPHSCALFEA 880


>ref|XP_020700621.1| isoamylase 2, chloroplastic [Dendrobium catenatum]
 gb|PKU77045.1| Isoamylase 2, chloroplastic [Dendrobium catenatum]
          Length = 894

 Score = 1025 bits (2651), Expect = 0.0
 Identities = 511/816 (62%), Positives = 613/816 (75%), Gaps = 13/816 (1%)
 Frame = +3

Query: 33   SHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLY 212
            + ++ E+ QP L +  + EK  + +S LFRTE  GLVKV VGA  MKY V IEV SLP  
Sbjct: 77   ARSTKEEAQPVLEMTSDFEKQNKGYSLLFRTEFDGLVKVFVGAIKMKYTVCIEVQSLPQS 136

Query: 213  LNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITL 392
            L  H+LIL WGMFRSDS   + PDS +S+L T++   +  +  ++TPF ++S   H + L
Sbjct: 137  LTSHNLILFWGMFRSDSLPFHGPDSHSSVLPTTKFKPEASQHLMKTPFVRDSC-RHILVL 195

Query: 393  EFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSEDGLV 572
            +FD   VP                +  I+THRKTNFCVPVG+  G PM LG S S DGL 
Sbjct: 196  DFDQNDVPFYLSFFLQSDGT--GQERTIRTHRKTNFCVPVGLGPGYPMALGVSSSNDGLA 253

Query: 573  NFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKG 752
            NF+LFSR A+SV LC Y+S  ++PSL+I+LDPY+N TGDIWHVS+ S+ NY+ YGYRCKG
Sbjct: 254  NFSLFSRNAKSVVLCFYKSKTSEPSLEIDLDPYVNHTGDIWHVSVHSITNYIGYGYRCKG 313

Query: 753  EITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVY----LGYLGDEPTFDWSGDVHPQL 920
            +I+WD GS  +A HVLLDPYAK I N   DQGES      LGYL  EP FDW+GD HPQ+
Sbjct: 314  DISWDGGSSSNAHHVLLDPYAKSIGNLSSDQGESACMVKCLGYLRKEPNFDWNGDTHPQI 373

Query: 921  PMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFPYDEK 1100
            PMEKL++YRLNV  FTK +SSEL  NVAGTFAGV++K+QH  +LG+NAVLLEP+F +D+K
Sbjct: 374  PMEKLIIYRLNVRQFTKSESSELAINVAGTFAGVVKKVQHLINLGINAVLLEPVFSFDKK 433

Query: 1101 RGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGE-GGTAC 1277
            RGPYFPYHFFSPM+ YG   + +S+V SMKEMVK LH+HGIEVLLEVVFTHT E G +  
Sbjct: 434  RGPYFPYHFFSPMSIYGGVDESISSVYSMKEMVKILHSHGIEVLLEVVFTHTCEVGDSDS 493

Query: 1278 QTISFRGIDNSSYYITEGGMG----SGMNCNNPIVQQLILDSLHYWLTEFHVDGFCFINS 1445
            Q IS  GIDN SYYI +G +     S + CN+PIVQQ+ILDSLH+W+TEFHVDGFCFINS
Sbjct: 494  QIISLLGIDNPSYYIVDGNVHLENCSALKCNDPIVQQMILDSLHHWVTEFHVDGFCFINS 553

Query: 1446 SYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRWAEINA 1625
            S LLQGSNGD L RPPLVE IAFDPLLS+TKII+DCW P+D S+    FPHW+RWAE+N+
Sbjct: 554  SSLLQGSNGDHLPRPPLVEDIAFDPLLSETKIISDCWSPIDMSYKYAIFPHWQRWAEMNS 613

Query: 1626 RFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVSFSSN 1805
            ++  DVRNFLRG+GLLS LATRLCGSGD+F+ SRGP +SFNY+TKN GL LVDLVSFS  
Sbjct: 614  KYFLDVRNFLRGQGLLSHLATRLCGSGDIFTASRGPAYSFNYITKNAGLTLVDLVSFSGA 673

Query: 1806 ELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTGGLFA 1985
            +LASELSWNCG EG T K  VLE+RL+Q+RNFLFILFISLG+PV NMGDECG+STGGL  
Sbjct: 674  DLASELSWNCGVEGPTEKNSVLEIRLRQVRNFLFILFISLGVPVINMGDECGYSTGGLPF 733

Query: 1986 YDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQSDPK 2165
            YD R PIDW  L+T +  QIT+F+AFL+SLR RRSD+FQ + F K +NI W+GSN S PK
Sbjct: 734  YDNRVPIDWKSLRTGFSIQITKFVAFLASLRNRRSDVFQRKIFYKAENITWYGSNLSLPK 793

Query: 2166 WEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCLVDTA 2345
            WEDPSCKFLAM L+AEK+ K  +S  GDLF+  N SDLPE V LP+   GY W  LVDT+
Sbjct: 794  WEDPSCKFLAMELKAEKDVKSRNSYNGDLFITINASDLPELVTLPESSAGYVWFRLVDTS 853

Query: 2346 LPYPGFFSMDSNFCD----GLSTYELKPHSCTLFEA 2441
            LPYP FFS DSN       GLS+YELKPH+C LFEA
Sbjct: 854  LPYPYFFSSDSNPNSQKNAGLSSYELKPHTCALFEA 889


>ref|XP_020087102.1| isoamylase 2, chloroplastic [Ananas comosus]
 ref|XP_020087103.1| isoamylase 2, chloroplastic [Ananas comosus]
          Length = 863

 Score = 1018 bits (2632), Expect = 0.0
 Identities = 509/825 (61%), Positives = 619/825 (75%), Gaps = 14/825 (1%)
 Frame = +3

Query: 24   SEASHNSTEQIQPGLGLNYNA-EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSS 200
            S AS +   ++ P +G+   + E+LQ+ FSY FRT+  G+VKV+V +   KYVV  EV+ 
Sbjct: 55   SSASRSPIRRLHPSVGVKIGSKEELQKGFSYTFRTDSNGIVKVVVRSEYGKYVVRFEVTF 114

Query: 201  LPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTH 380
            LP +  E  L+L+W MF SDSS L  P SQ S           G  + + PF QNS G+ 
Sbjct: 115  LPQFGTEEGLVLNWTMFTSDSSLLLVPCSQASE----------GTVTAKVPFVQNSSGSF 164

Query: 381  AITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSE 560
               L FD+++VP              +  S I++HRKTNFCVPVG+ SG+PMPLG S S+
Sbjct: 165  LAELVFDASKVPFYLSFLLN------SAGSEIRSHRKTNFCVPVGLGSGQPMPLGVSVSD 218

Query: 561  DGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGY 740
            DGL NF+LFS+ AE V LCLY+   ++P+L+IELDPYIN TGDIWHVS+E+V  YVSYGY
Sbjct: 219  DGLTNFSLFSKNAEGVILCLYDGIKDEPALEIELDPYINCTGDIWHVSLENVEEYVSYGY 278

Query: 741  RCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVYLGY----LGDEPTFDWSGDV 908
            RCKG I W  G RFH  HVLLDPYAK+I NF PD G  V L      L  E  FDWSGD 
Sbjct: 279  RCKGPILWRKGDRFHMKHVLLDPYAKVIGNFFPDHGGKVSLSRCLASLAKESAFDWSGDT 338

Query: 909  HPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFP 1088
             P LPMEKLVVYRLNVG  TKDKSS L EN AGTF+G+IEK+++FK+LGVNAVLLEPIFP
Sbjct: 339  SPCLPMEKLVVYRLNVGLCTKDKSSGLSENAAGTFSGLIEKIEYFKTLGVNAVLLEPIFP 398

Query: 1089 YDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEGG 1268
            +DEK+GPYFP+HFFSPM SYG  RD  S   SMKEM+K++HA G+EVLLEVVF+HT EGG
Sbjct: 399  FDEKKGPYFPFHFFSPMMSYGHGRDSASGTNSMKEMIKSMHARGMEVLLEVVFSHTSEGG 458

Query: 1269 TAC-QTISFRGIDNSSYYITEGGMGSG----MNCNNPIVQQLILDSLHYWLTEFHVDGFC 1433
             A  Q ISFRGIDNSSYYI +G + SG    +NCNNPIVQ+LILDSLH+W+ E+HVDGFC
Sbjct: 459  DAASQMISFRGIDNSSYYIVDGDVRSGANNALNCNNPIVQRLILDSLHHWVVEYHVDGFC 518

Query: 1434 FINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRWA 1613
            F+NSS L++ SNG+ LSRPPL+EAIAFDP+LSKTKIIADCW P+D S  +I FPHW++WA
Sbjct: 519  FVNSSSLVRSSNGNNLSRPPLLEAIAFDPILSKTKIIADCWSPIDMSSMEIQFPHWKKWA 578

Query: 1614 EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 1793
            E+N+RF  DVRNFLRGE LLSDLATR+CGSGDLFS SRGP +SFNY+TKNFGLPLVDLVS
Sbjct: 579  EMNSRFSVDVRNFLRGEALLSDLATRICGSGDLFS-SRGPAYSFNYITKNFGLPLVDLVS 637

Query: 1794 FSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTG 1973
            FSS +LASELSWNCGEEG T    VL+ RLKQIRNFLFILF+SLG+PV NMGDECG+S G
Sbjct: 638  FSSIDLASELSWNCGEEGPTGNSSVLQTRLKQIRNFLFILFVSLGVPVLNMGDECGYSNG 697

Query: 1974 GLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQ 2153
            G  +Y+ R+P DWN L+T +G QITQF+A+LSSLR RR D+FQ +DFLKV+NI W+GSNQ
Sbjct: 698  GSPSYNDRRPFDWNSLRTGFGLQITQFVAYLSSLRNRRGDIFQRKDFLKVENICWNGSNQ 757

Query: 2154 SDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCL 2333
            ++P W D SCKFL++  +A+ +G+     +GDLF+ FN SD PE  +LP+  EG  W+ L
Sbjct: 758  TEPNWGDSSCKFLSVMFKADVDGRASKLHRGDLFIGFNASDHPEVALLPEQSEGTVWLRL 817

Query: 2334 VDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEAGMSDL 2456
            VDTALP+PGFF+  S    +   GLS YE+K HSC+LFEA  ++L
Sbjct: 818  VDTALPFPGFFASYSDPNVHQVAGLSAYEIKSHSCSLFEAKRTEL 862


>ref|XP_010278620.1| PREDICTED: isoamylase 2, chloroplastic [Nelumbo nucifera]
 ref|XP_010278628.1| PREDICTED: isoamylase 2, chloroplastic [Nelumbo nucifera]
          Length = 890

 Score = 1014 bits (2621), Expect = 0.0
 Identities = 509/827 (61%), Positives = 612/827 (74%), Gaps = 17/827 (2%)
 Frame = +3

Query: 12   NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 191
            N+KI  +S +S +Q +P LG     E++++  +YLFRTE+GG VK+ VG  N  Y V IE
Sbjct: 66   NLKIFASSPSSVDQTRPSLGTFTKTEEVEKPLTYLFRTEIGGQVKITVGRKNTNYTVCIE 125

Query: 192  VSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 371
            VSSLP    E  L L+WG+FRSDSS L  P SQ S  +T  ST +       TP  Q S 
Sbjct: 126  VSSLPQCNTEDKLFLNWGIFRSDSSCLTLPGSQVSAPETHSSTME-------TPLMQKSS 178

Query: 372  GTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 551
            G H + LEF+S Q P                +S I++HRKTNFCVPVGISSG P PLG S
Sbjct: 179  GRHFLQLEFESNQAPFYLSFMLLFSSNTATNNSEIRSHRKTNFCVPVGISSGHPAPLGIS 238

Query: 552  KSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVS 731
             S+DG VNF+LFSR AESV LCLY+  ++QPSL+I+LDPYINRTG+IWHVSMESV  YVS
Sbjct: 239  FSDDGSVNFSLFSRNAESVVLCLYDEKSDQPSLEIDLDPYINRTGNIWHVSMESVAPYVS 298

Query: 732  YGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGES----VYLGYLGDEPTFDWS 899
            YGYRCKG+I    G RF A +VLLDPYAK++ + +P+  E+      LG+L   PTFDWS
Sbjct: 299  YGYRCKGDIHLKKGDRFDARNVLLDPYAKILSSSLPNHSETHSPPKRLGHLCKIPTFDWS 358

Query: 900  GDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEP 1079
            GD+ P L +E+LVVYRLNV  FT+DKS +LP +V GTF+G+IEKL HFKSLGVNAVLLEP
Sbjct: 359  GDIRPCLEIEELVVYRLNVRRFTEDKSCQLPADVLGTFSGLIEKLHHFKSLGVNAVLLEP 418

Query: 1080 IFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTG 1259
            +F + E+ GPYFPYHFFS  + YG + D +ST+ SMKEM+K LHA+GIEVLLEVVFTH  
Sbjct: 419  VFSFGEQNGPYFPYHFFSASDLYGPSYDNVSTINSMKEMIKRLHANGIEVLLEVVFTHAA 478

Query: 1260 EGG-TACQTISFRGIDNSSYYITEG----GMGSGMNCNNPIVQQLILDSLHYWLTEFHVD 1424
            EGG ++ QTISFRGIDNSSYYI  G    G  + +NCNNPIVQ++ILD L YW+TEFH+D
Sbjct: 479  EGGDSSFQTISFRGIDNSSYYILNGDTQLGTRNALNCNNPIVQRMILDILQYWVTEFHID 538

Query: 1425 GFCFINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWR 1604
            GFCF+N+S LL+G NG+ LSRPPLVEAIAFDPLLSKTKIIADCW P +    DI FPHW+
Sbjct: 539  GFCFMNASSLLRGLNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPCEMVTRDIRFPHWK 598

Query: 1605 RWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVD 1784
            RWAE+N RFC D+R FLRGEGLLSDLATRLCGSGD+F D RGP FSFN++ +NFGLPLVD
Sbjct: 599  RWAEVNNRFCHDIRKFLRGEGLLSDLATRLCGSGDIFLDGRGPAFSFNFIARNFGLPLVD 658

Query: 1785 LVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGF 1964
            LVSFS NE++SELSWNCGEEGATN  +VLE RLKQIRNFLFIL+IS G+P+ NMGDECG 
Sbjct: 659  LVSFSGNEVSSELSWNCGEEGATNNTVVLERRLKQIRNFLFILYISFGVPILNMGDECGQ 718

Query: 1965 STGGLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHG 2144
            S+ G  +Y  R+P DW  L+T +  Q T+FIAFLSSLR RRSDL Q R+FLKV+NI+W+G
Sbjct: 719  SSSGSTSYGDRKPFDWTALRTGFAIQTTEFIAFLSSLRTRRSDLLQKRNFLKVENIEWYG 778

Query: 2145 SNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSD----KGDLFVCFNGSDLPESVILPQLPE 2312
            SNQS P+WEDPS KFLA+ L+++ +     SD    +GDLF+ FN     E VILP   E
Sbjct: 779  SNQSQPRWEDPSSKFLALRLKSDIDNSQSDSDSSQIRGDLFIAFNAGGHSEGVILPSPSE 838

Query: 2313 GYAWVCLVDTALPYPGFFSMDSN----FCDGLSTYELKPHSCTLFEA 2441
            G  W+ LVDTALP+PGFFS D +       GL  YE+K HSC LFEA
Sbjct: 839  GMVWLRLVDTALPFPGFFSNDGDPVLEQMQGLIAYEMKAHSCALFEA 885


>gb|OAY83378.1| Isoamylase 2, chloroplastic [Ananas comosus]
          Length = 1205

 Score = 1007 bits (2604), Expect = 0.0
 Identities = 507/837 (60%), Positives = 617/837 (73%), Gaps = 14/837 (1%)
 Frame = +3

Query: 24   SEASHNSTEQIQPGLGLNYNA-EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSS 200
            S AS +   ++ P +G+   + E+LQ+ FSY FRT+  G+VKV+V +   KYVV  EV+ 
Sbjct: 55   SSASRSPIRRLHPSVGVKIGSKEELQKGFSYTFRTDSNGIVKVVVRSEYGKYVVRFEVTF 114

Query: 201  LPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTH 380
            LP +  E  L+L+W MF SDSS L  P SQ S           G  + + PF QNS G+ 
Sbjct: 115  LPQFGTEEGLVLNWTMFTSDSSLLLVPCSQASE----------GTVTAKVPFVQNSSGSF 164

Query: 381  AITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSE 560
               L FD+++VP              +  S I++HRKTNFCVPVG+ SG+PMPLG S S+
Sbjct: 165  LAELVFDASKVPFYLSFLLN------SAGSEIRSHRKTNFCVPVGLGSGQPMPLGVSVSD 218

Query: 561  DGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGY 740
            DGL NF+LFS+ AE V LCLY+   ++P+L+IELDPYIN TGDIWHVS+E+V  YVSYGY
Sbjct: 219  DGLTNFSLFSKNAEGVILCLYDGIKDEPALEIELDPYINCTGDIWHVSLENVEEYVSYGY 278

Query: 741  RCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVYLGY----LGDEPTFDWSGDV 908
            RCKG I W  G RFH  HVLLDPYAK+I NF PD G  V L      L  E  FDWSGD 
Sbjct: 279  RCKGPILWRKGDRFHMKHVLLDPYAKVIGNFFPDHGGKVSLSRCLASLAKESAFDWSGDT 338

Query: 909  HPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFP 1088
             P LPMEKLVVYRLNVG  TKDKSS L EN AGTF+G+IEK+++FK+LGVNAVLLEPIFP
Sbjct: 339  SPCLPMEKLVVYRLNVGLCTKDKSSGLSENAAGTFSGLIEKIEYFKTLGVNAVLLEPIFP 398

Query: 1089 YDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEGG 1268
            +DEK+GPYFP+HFFSPM SYG  RD  S   SMKEM+K++HA G+EVLLEVVF+HT EGG
Sbjct: 399  FDEKKGPYFPFHFFSPMMSYGHGRDSASGTNSMKEMIKSMHARGMEVLLEVVFSHTSEGG 458

Query: 1269 TAC-QTISFRGIDNSSYYITEGGMGSG----MNCNNPIVQQLILDSLHYWLTEFHVDGFC 1433
             A  Q ISFRGIDNSSYYI +G + SG    +NCNNPIVQ+LILDSLH+W+ E+HVDGFC
Sbjct: 459  DAASQMISFRGIDNSSYYIVDGDVRSGANNALNCNNPIVQRLILDSLHHWVVEYHVDGFC 518

Query: 1434 FINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRWA 1613
            F+NSS L++ SNG+ LSRPPL+EAIAFDP+LSKTKIIADCW P+D S  +I FPHW++WA
Sbjct: 519  FVNSSSLVRSSNGNNLSRPPLLEAIAFDPILSKTKIIADCWSPIDMSSMEIQFPHWKKWA 578

Query: 1614 EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 1793
            E+N+RF  DVRNFLRGE LLSDLATR+CGSGDLFS SRGP +SFNY+TKNFGLPLVDLVS
Sbjct: 579  EMNSRFSVDVRNFLRGEALLSDLATRICGSGDLFS-SRGPAYSFNYITKNFGLPLVDLVS 637

Query: 1794 FSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTG 1973
            FSS +LASELSWNCGEEG T    VL+ RLKQIRNFLFILF+SLG+PV NMGDECG+S G
Sbjct: 638  FSSIDLASELSWNCGEEGPTGNSSVLQTRLKQIRNFLFILFVSLGVPVLNMGDECGYSNG 697

Query: 1974 GLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQ 2153
            G  +Y+ R+P DWN L+T +G QITQF+A+LSSLR RR D+FQ +DFLKV+NI W+GSNQ
Sbjct: 698  GSPSYNDRRPFDWNSLRTGFGLQITQFVAYLSSLRNRRGDIFQRKDFLKVENICWNGSNQ 757

Query: 2154 SDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCL 2333
            ++P W D SCKFL++  +A+ +G+     +GDLF+ FN SD PE  +LP+  EG  W+ L
Sbjct: 758  TEPNWGDSSCKFLSVMFKADADGRASKLHRGDLFIGFNASDHPEVALLPEQSEGTVWLRL 817

Query: 2334 VDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEAGMSDL*FKVNLIEITSV 2492
            VDTALP+PGFF+  S    +   GLS YE+K H    F    S   F+ N +   S+
Sbjct: 818  VDTALPFPGFFASYSDPNVHQVAGLSAYEIKSHRINTFAHTPSKEWFQTNAVLCVSL 874


>ref|XP_018683242.1| PREDICTED: isoamylase 2, chloroplastic-like [Musa acuminata subsp.
            malaccensis]
          Length = 862

 Score =  983 bits (2542), Expect = 0.0
 Identities = 502/828 (60%), Positives = 608/828 (73%), Gaps = 18/828 (2%)
 Frame = +3

Query: 12   NVKISEASHNSTEQIQPGLGLNYNAEKLQ-RVFSYLFRTELGGLVKVLV-GANNMKYVVN 185
            N  +   S  S  Q +P    +  A+ L+ R F Y+FR+E G LVKV+V GA  + + V+
Sbjct: 54   NSVVRATSPISLRQSRPMAVASGKAQVLEGREFPYMFRSEKGDLVKVVVVGAVGVNFSVH 113

Query: 186  IEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQN 365
            IEV S+    ++ +L+L + M RSD       D +             G  ++  PF +N
Sbjct: 114  IEVLSV----SQDELVLIYEMSRSDPPGSLVADIEK------------GIGAVIMPFVRN 157

Query: 366  SLGTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLG 545
            S G + + L+FDSA+ P                 S IKTHRKT FCVPVG+  G P PLG
Sbjct: 158  SSGRYTLDLDFDSAKAPFYLSFMLHLCSDAGVVASEIKTHRKTRFCVPVGLGPGYPAPLG 217

Query: 546  FSKSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNY 725
             S S+DG+VNF+LFSRKAE+V LCLYE     PSL+IELDPY+NRTGDIWHVSMES+ +Y
Sbjct: 218  ASVSDDGMVNFSLFSRKAENVVLCLYEGKTEVPSLEIELDPYVNRTGDIWHVSMESIEDY 277

Query: 726  VSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVY----LGYLGDEPTFD 893
            VSYGYRCKG +    G  F   HVLLDPYAK+++N +  QG+++     LG L  EP FD
Sbjct: 278  VSYGYRCKGPVEKRGG--FDMQHVLLDPYAKMVRNLLSVQGDTMTPTKCLGSLEMEPIFD 335

Query: 894  WSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLL 1073
            WSGDVHPQLP EKLVVYRLNVG FT D SS LPE+VAGTF GV EK++HFK LGVNA+LL
Sbjct: 336  WSGDVHPQLPTEKLVVYRLNVGQFTIDNSSGLPEDVAGTFGGVSEKVEHFKELGVNAILL 395

Query: 1074 EPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTH 1253
            EPIFP+D  +GPYFPYHFFS M+ YG   +  S + SMKEM+K LH+ GIEVL+EVVFTH
Sbjct: 396  EPIFPFDVNKGPYFPYHFFSVMDEYGQEHNAASAINSMKEMIKTLHSEGIEVLMEVVFTH 455

Query: 1254 TGEGGTA-CQTISFRGIDNSSYYITEGGMGSGMN----CNNPIVQQLILDSLHYWLTEFH 1418
            + EGG A    ISFRGID+SSYYI +  +GSG N    CNNPIVQQLI+DSL +W+ EFH
Sbjct: 456  SAEGGDADSHVISFRGIDSSSYYIVDKNVGSGTNSLLKCNNPIVQQLIIDSLRHWVVEFH 515

Query: 1419 VDGFCFINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPH 1598
            VDGFCFINSS + +G NGD LSRPPLVEAIAFDP+LS+TKI+ADCW P+D S+ +I FPH
Sbjct: 516  VDGFCFINSSSMARGQNGDHLSRPPLVEAIAFDPVLSRTKIVADCWSPLDMSYMEIQFPH 575

Query: 1599 WRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPL 1778
            W+ WAE+N RFC DVRNFLRGEGLLS+LATRLCGSGDLFS SRGP FSFN+VTKN GLPL
Sbjct: 576  WKAWAEMNMRFCSDVRNFLRGEGLLSNLATRLCGSGDLFS-SRGPAFSFNFVTKNVGLPL 634

Query: 1779 VDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDEC 1958
            VDLVSFS+ ELASELSWNCG+EG TN   VLE RLKQIRNFLF+LF+SLG+PV NMGDEC
Sbjct: 635  VDLVSFSTAELASELSWNCGDEGPTNNNTVLETRLKQIRNFLFVLFVSLGVPVLNMGDEC 694

Query: 1959 GFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDW 2138
            G+STGG   YD R+PI+W+ L T + KQIT+FIA+L SLRIRR D+FQ + FLKV+NI W
Sbjct: 695  GYSTGGSPLYDDRKPINWDSLGTGFSKQITKFIAYLGSLRIRRGDIFQSKHFLKVENIVW 754

Query: 2139 HGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGY 2318
             GSNQS+PKW+DP+CKFLA+ L++EKN  + +S+ GDLF+CFN S+  E+V+LP+ PEG 
Sbjct: 755  FGSNQSEPKWDDPTCKFLALALKSEKNFDMSNSNCGDLFICFNASNNLETVVLPEQPEGN 814

Query: 2319 AWVCLVDTALPYPGFFSMDSNFCD-------GLSTYELKPHSCTLFEA 2441
             W+ LVDT+L +PG F   SN CD       G S+YELKPHSC LFEA
Sbjct: 815  VWLRLVDTSLAFPGIF---SNSCDPNVQKAEGSSSYELKPHSCALFEA 859


>gb|PIA39269.1| hypothetical protein AQUCO_02600010v1 [Aquilegia coerulea]
          Length = 886

 Score =  968 bits (2503), Expect = 0.0
 Identities = 488/826 (59%), Positives = 595/826 (72%), Gaps = 15/826 (1%)
 Frame = +3

Query: 18   KISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVS 197
            K+S  S  S+  I   L  + + E L+    YLFRT+ GG VKV VG  +MKY+V IEVS
Sbjct: 68   KVSALSQGSSYPIGTQLYKSADLEDLENASMYLFRTDAGGQVKVYVGRKHMKYIVYIEVS 127

Query: 198  SLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGT 377
             LP  +++ +L+LSWG++RSDSS +       + LD   STS    +  +TPF QNSLG 
Sbjct: 128  FLPQCVSKKELLLSWGVYRSDSSAM-------NPLDPQSSTSGAKSSISQTPFPQNSLGA 180

Query: 378  HAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKS 557
              + +EFDS Q P              +    IK+HRKTNFCVPVG+ SG P PLG S S
Sbjct: 181  RTLQMEFDSIQAPFYFSFLLLCPSSAASDLKEIKSHRKTNFCVPVGLGSGSPAPLGISFS 240

Query: 558  EDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYG 737
             DG VNFALFSR AE+V LCLY+    + SL+I+LDPYINRTGDIWHVSMESV  Y+SYG
Sbjct: 241  NDGSVNFALFSRNAENVVLCLYDEMEGKLSLEIDLDPYINRTGDIWHVSMESVAPYMSYG 300

Query: 738  YRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESV----YLGYLGDEPTFDWSGD 905
            YRC+G I  + G+  H SHVLLDPYAK++     DQ  SV    YLG L  EP+FDWSG+
Sbjct: 301  YRCRGTIPREKGNICHESHVLLDPYAKVLHRSFTDQSGSVPLLRYLGNLVKEPSFDWSGE 360

Query: 906  VHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIF 1085
            + P LP+EKLVVYRLNV  FT+DKSS L   VAGTF+G+IEK+ HFK LGVNA+LLEPIF
Sbjct: 361  MRPCLPLEKLVVYRLNVECFTEDKSSRLSSAVAGTFSGLIEKMHHFKRLGVNAILLEPIF 420

Query: 1086 PYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEG 1265
             +DE++GPYFPYHFFSPMNSYG TRDG+S   SMKEMVK +H + IE+LLEVVFTHT E 
Sbjct: 421  SFDEQKGPYFPYHFFSPMNSYGPTRDGVSAASSMKEMVKVMHENNIEILLEVVFTHTAE- 479

Query: 1266 GTACQTISFRGIDNSSYYI----TEGGMGSGMNCNNPIVQQLILDSLHYWLTEFHVDGFC 1433
              A QTI  +GID+ SYY+     E    S +NCN PIVQ+LI+DSL YW+TEFHVDGFC
Sbjct: 480  -CAAQTIGLQGIDDKSYYVFDEDVESNDRSALNCNYPIVQRLIVDSLRYWVTEFHVDGFC 538

Query: 1434 FINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRWA 1613
            FIN+S LL+G NG+ LSRPPL+EAIAFDPLLSKTK+IAD   P  +   DI FPHW+RWA
Sbjct: 539  FINASSLLRGPNGEYLSRPPLIEAIAFDPLLSKTKVIADSLGPYSSDSKDIRFPHWKRWA 598

Query: 1614 EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 1793
            E+N +FC DVRNFLRGEGLLS+LATRLCGS D+F D RGP F+FN++ +NFGLPLVDLVS
Sbjct: 599  EMNVKFCSDVRNFLRGEGLLSNLATRLCGSADIFLDGRGPAFAFNFIARNFGLPLVDLVS 658

Query: 1794 FSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTG 1973
            FSS +L+SELSWNCGEEG TNK +VLE RLKQIRN LFIL++S+G+PV NMGDECG S+G
Sbjct: 659  FSSGKLSSELSWNCGEEGPTNKAVVLERRLKQIRNSLFILYLSMGVPVLNMGDECGQSSG 718

Query: 1974 GLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQ 2153
            G  +Y  R+  DW+ L T +G Q+T FI+FLSS+R+RRSD  Q ++F++V++++WHGS+ 
Sbjct: 719  GSPSYSDRKSFDWDALSTTFGVQVTDFISFLSSVRMRRSDFLQRKNFMRVEDVEWHGSDL 778

Query: 2154 SDPKWEDPSCKFLAMTLRAEKNGKLPSSDK---GDLFVCFNGSDLPESVILPQLPEGYAW 2324
            + P WEDPS K LAMTL+A      P+S K    D F+  N +D  E+VILPQ  +G AW
Sbjct: 779  AQPSWEDPSNKLLAMTLKANLKSSKPNSKKSRGSDFFIALNANDSSETVILPQPSQGMAW 838

Query: 2325 VCLVDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEAGMS 2450
              LVDTALP+PGFF  D         G   YE+K HSC LFEA  S
Sbjct: 839  FRLVDTALPFPGFFRTDGEPVIEQTPGFLAYEMKSHSCALFEAHSS 884


>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  964 bits (2492), Expect = 0.0
 Identities = 491/829 (59%), Positives = 601/829 (72%), Gaps = 15/829 (1%)
 Frame = +3

Query: 12   NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 191
            N K+S  SH + E+ +        +E+L+   +YLF TE+GG VKV+VG  N KY+V+IE
Sbjct: 68   NWKVSATSHIAIEETENRYSTT-ESEELESRLNYLFLTEIGGQVKVIVGKKNKKYIVSIE 126

Query: 192  VSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 371
            VSSL LY +++ LILSWG+FRS+SS     D Q  + +   +T+++       PF + S 
Sbjct: 127  VSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEI-------PFMERSS 179

Query: 372  GTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 551
            G+ A+ L+F++   P               + S I++HRKTNFC+PVG   G P PLG S
Sbjct: 180  GSFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLS 239

Query: 552  KSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVS 731
             S DG  NFA FSR A  V LCLY+  +++P+L+I+LDPY+NRTGDIWH SMESVG++VS
Sbjct: 240  FSSDGSPNFAFFSRNAGGVVLCLYDGTSDKPALEIDLDPYVNRTGDIWHASMESVGSFVS 299

Query: 732  YGYRCKGEITWDNGSRFHASHVLLDPYAKLIKN-FIPDQG--ESVYLGYLGDEPTFDWSG 902
            YGYRCK     D+G   H  HV LDPYAKLI+N F  D G      LG L  EP F+W+ 
Sbjct: 300  YGYRCKEANLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGLKPQPRLGELQKEPAFNWND 359

Query: 903  DVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPI 1082
            DVHP +PMEKLVVYRLNV  FTKD+SS++  ++AGTF+G++EKL HFK LGVNAVLLEPI
Sbjct: 360  DVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPI 419

Query: 1083 FPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGE 1262
            F +DE++GPYFP+HFFSPMN YG +   +ST+ S+KEMVK LHA+GIEVLLEVVFTHT E
Sbjct: 420  FSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTHTAE 479

Query: 1263 GGTACQTISFRGIDNSSYYITEG----GMGSGMNCNNPIVQQLILDSLHYWLTEFHVDGF 1430
             G      + +GID+S YY   G    G+ + +NCN  IVQQ+I+DSL YW+TEFHVDGF
Sbjct: 480  SG------ALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHVDGF 533

Query: 1431 CFINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRW 1610
            CFIN+S LL+G +G+ LSRPPLVE IAFDPLLSKTKIIADCW P +    +I FPHW+RW
Sbjct: 534  CFINASSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHWKRW 593

Query: 1611 AEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLV 1790
            AE+N RFC DVRNFLRGEGL SD ATRLCGSGD+F D RGP FSFN+ TKNFGLPLVDLV
Sbjct: 594  AEVNTRFCNDVRNFLRGEGL-SDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLVDLV 652

Query: 1791 SFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFST 1970
            SFSS+ELASELSWNCG+EG TNK  VLE RLKQIRNFLFIL++SLG+P+ NMGDECG S+
Sbjct: 653  SFSSSELASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECGQSS 712

Query: 1971 GGLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSN 2150
            GG  AY  R+P +WN +KT +G Q  QFI+FLSSLR RRSDL Q R FLK ++IDWHGS+
Sbjct: 713  GGSPAYGDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWHGSD 772

Query: 2151 QSDPKWEDPSCKFLAMTLRAEK-NGKLP---SSDKGDLFVCFNGSDLPESVILPQLPEGY 2318
            QS P+W+DPS KFLAMTL+AE   G+LP   SS KGDLF+ FN +D    VILP  P G 
Sbjct: 773  QSPPRWDDPSSKFLAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPPTGM 832

Query: 2319 AWVCLVDTALPYPGFFSMDSNFC----DGLSTYELKPHSCTLFEAGMSD 2453
             W  LVDTALP+PGFF+ D         GL TY+++ HSC LFEA   D
Sbjct: 833  VWHRLVDTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEANTLD 881


>ref|XP_020592870.1| isoamylase 2, chloroplastic [Phalaenopsis equestris]
          Length = 861

 Score =  959 bits (2479), Expect = 0.0
 Identities = 483/819 (58%), Positives = 603/819 (73%), Gaps = 21/819 (2%)
 Frame = +3

Query: 48   EQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHD 227
            E+ QP L      EK  + F +LFRTE GGLVKV VGA  MKY V IEV SLP  +  H+
Sbjct: 78   EEAQPML------EKQHKGFKFLFRTEFGGLVKVFVGAMEMKYTVCIEVHSLPQSMINHN 131

Query: 228  LILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSA 407
            LIL WGMFRSDSS     DS+ S+            +S++TPF   S       L+FD +
Sbjct: 132  LILLWGMFRSDSSVFQGSDSRISV------------SSMKTPFVGESC-----RLDFDHS 174

Query: 408  QVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSEDGLVNFALF 587
             VP                +S I+THRKTNFCVPVG+  G PMPLG S S DG  NF+LF
Sbjct: 175  NVPFYLSFLLLQPDDT-GFESAIRTHRKTNFCVPVGLGPGHPMPLGVSLSNDGWTNFSLF 233

Query: 588  SRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWD 767
            SR A++V LCLYES  N+P+L++ELDPY+NRTGD+WHVS++++ NY+ YGYRCKG+I   
Sbjct: 234  SRNAKTVVLCLYESETNEPTLELELDPYVNRTGDVWHVSIDNITNYILYGYRCKGDI--- 290

Query: 768  NGSRFHASHVLLDPYAKLIKNFIP-DQGESVY----LGYLGDEPTFDWSGDVHPQLPMEK 932
                 HA HVLLDPYAK+I+N    D G+S      LGYL  +P FDWSG+  P +PMEK
Sbjct: 291  -----HAHHVLLDPYAKIIRNLSSSDHGDSESMVKSLGYLRKDPNFDWSGNTQPHIPMEK 345

Query: 933  LVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFPYDEKRGPY 1112
            LVVYRLNV +FTK +SS +  N+AGTFAGV +K+QH K+LG+NAVLLEP+FP+++K GPY
Sbjct: 346  LVVYRLNVENFTKSESSGVDINIAGTFAGVAKKVQHLKNLGINAVLLEPVFPFNKKEGPY 405

Query: 1113 FP-------YHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGE-GG 1268
            FP       YHFFSPM+  G        + S+KEMVK LH+HGIEVLLEVVFTHT E G 
Sbjct: 406  FPXXXXYFPYHFFSPMSMCG-------NMYSLKEMVKILHSHGIEVLLEVVFTHTCEVGD 458

Query: 1269 TACQTISFRGIDNSSYYITEGGM----GSGMNCNNPIVQQLILDSLHYWLTEFHVDGFCF 1436
            +  + IS  GIDNSSYY+ +G +     S + CNN IV Q+ILDSLH+W+TE+H+DGFCF
Sbjct: 459  SESERISLLGIDNSSYYVVDGNVISENCSRLKCNNAIVAQMILDSLHHWVTEYHIDGFCF 518

Query: 1437 INSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRWAE 1616
            +NSS LL+GSNG+ L RPPL+E IAFDPLLS+TKII+DCW P+D S+ D  FPHW+RWAE
Sbjct: 519  VNSSSLLEGSNGNHLPRPPLIEDIAFDPLLSETKIISDCWSPIDISYKDAIFPHWQRWAE 578

Query: 1617 INARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVSF 1796
            +N+++  D+RNFLRG+GL+S LATRLCGSGD+F+DSRGP  SFNY+T+N GL LVDLVS+
Sbjct: 579  MNSKYFHDIRNFLRGQGLISQLATRLCGSGDMFTDSRGPGHSFNYITRNVGLTLVDLVSY 638

Query: 1797 SSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTGG 1976
            + ++LASELSWNCGEEG T++  VLE+RL+QIRNFLF+LF+SLG+PV NMGDECG S+ G
Sbjct: 639  NGSDLASELSWNCGEEGLTDENSVLEIRLRQIRNFLFLLFVSLGVPVINMGDECGHSSSG 698

Query: 1977 LFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQS 2156
              +Y+ + PIDW+ L T + +QIT+F+AFL+SLR RRSD+FQ ++F K +NI WHGSNQS
Sbjct: 699  SPSYN-KGPIDWSSLGTKFSEQITKFVAFLASLRDRRSDIFQRKEFYKAENIAWHGSNQS 757

Query: 2157 DPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCLV 2336
             PKWEDPSCKFLAM L+AEK+ +LP+S  GDLF+  NG DL E+V LP+ PEG+ W  LV
Sbjct: 758  PPKWEDPSCKFLAMELKAEKDVRLPNSHNGDLFIVVNGGDLLETVTLPESPEGFIWFRLV 817

Query: 2337 DTALPYPGFFSMDSN----FCDGLSTYELKPHSCTLFEA 2441
            DT+ PYPGFFS DSN      +GLS+YELKPH+C LFEA
Sbjct: 818  DTSRPYPGFFSSDSNPNSRKIEGLSSYELKPHACALFEA 856


>ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber]
 ref|XP_023886632.1| isoamylase 2, chloroplastic [Quercus suber]
 gb|POE68275.1| isoamylase 2, chloroplastic [Quercus suber]
          Length = 887

 Score =  954 bits (2466), Expect = 0.0
 Identities = 486/830 (58%), Positives = 591/830 (71%), Gaps = 17/830 (2%)
 Frame = +3

Query: 12   NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 191
            N ++   S    E+I+  +     A+  ++  +YLF TE+GG VKV V   ++KY VNIE
Sbjct: 68   NSRVFATSRVLVEEIEQKVSTITEADNREKALTYLFWTEVGGQVKVFVRKTSVKYAVNIE 127

Query: 192  VSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 371
            VSSL L  +E  LI SWG +R+DSS     D Q+S+ D+   TS+       TPF Q S 
Sbjct: 128  VSSLQLQSSEDRLISSWGRYRADSSSFIPLDVQSSVPDSRTPTSE-------TPFIQTSA 180

Query: 372  GTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 551
            G  A+ LEF++ Q+P              +  S I++HRKT FCVPVG   G P PLG S
Sbjct: 181  GRFALELEFEAKQIPFYLSFILKLPSDSGSRGSEIRSHRKTKFCVPVGFRKGYPAPLGLS 240

Query: 552  KSEDGLVNFALFSRKAESVNLCLYESG-ANQPSLDIELDPYINRTGDIWHVSMESVGNYV 728
             S DG +NFA+FSR  E V LC Y+   A +P+L+++LDPYINR+GDIWH S ES   +V
Sbjct: 241  FSPDGSMNFAIFSRNVEGVILCFYDDAKAEEPALELDLDPYINRSGDIWHASFESAWTFV 300

Query: 729  SYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQ-GESVYLGYLGDEPTFDWSGD 905
            SYGY+CKG +   N ++F A  ++LDPYAK+I N IP   G   YLG L  EP FDW  D
Sbjct: 301  SYGYQCKGSLLQRNNNKFDAGQIILDPYAKIIVNSIPSSDGSQKYLGRLCKEPGFDWGDD 360

Query: 906  VHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIF 1085
             HP LPMEKLVVYRLNV  FT+ KSS+L  +VAGTF G+ EKLQHFK LG+NAVLLEPIF
Sbjct: 361  THPYLPMEKLVVYRLNVRHFTEHKSSQLATDVAGTFIGLTEKLQHFKDLGMNAVLLEPIF 420

Query: 1086 PYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEG 1265
             +DEK GPYFP HFFSP N YG +   +S + SMKEMVK LHA+GIEVLLEVVFTHT   
Sbjct: 421  SFDEKNGPYFPCHFFSPTNLYGPSGGSVSAINSMKEMVKKLHANGIEVLLEVVFTHTAVA 480

Query: 1266 GTACQTISFRGIDNSSYYITEGGMG----SGMNCNNPIVQQLILDSLHYWLTEFHVDGFC 1433
            G      S +GID+ SYY    G      + +NCN PIVQQLILDSL YW+TEFH+DGFC
Sbjct: 481  G------SLQGIDDISYYHVNEGEDLEATNYLNCNYPIVQQLILDSLQYWVTEFHIDGFC 534

Query: 1434 FINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRWA 1613
            FIN+S LL+G +G+ LSRPPLVEAI+FDPLLSKTKIIADCW P D    +  FPHW+RWA
Sbjct: 535  FINASSLLRGFHGEYLSRPPLVEAISFDPLLSKTKIIADCWDPHDMLPKETRFPHWKRWA 594

Query: 1614 EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 1793
            EI+ +FC DVRNF RGEGLLSDLATRLCGSGD+FSD RGP FSFN++ +NFGL LVDLVS
Sbjct: 595  EIDTKFCNDVRNFWRGEGLLSDLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVS 654

Query: 1794 FSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTG 1973
            FS++ LAS+LSWNCGEEG T+   VLE RLKQIRNFLF+L+ISLG+P+ NMGDECG S G
Sbjct: 655  FSNDALASQLSWNCGEEGPTDNTTVLERRLKQIRNFLFVLYISLGVPILNMGDECGQSCG 714

Query: 1974 GLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQ 2153
            G  AY  R+P+DWN LKT +  Q +QFI+FLSSLR+RRSDL Q R+FLK +NI+WHGS+Q
Sbjct: 715  GSPAYSDRKPLDWNTLKTGFSIQTSQFISFLSSLRMRRSDLLQKRNFLKEENIEWHGSDQ 774

Query: 2154 SDPKWEDPSCKFLAMTLRAEKNGKLPSSD------KGDLFVCFNGSDLPESVILPQLPEG 2315
            S PKWEDPSC+FLAMTLRA++     +S+      +GDLF+ FN +DL ESVILPQ PEG
Sbjct: 775  SPPKWEDPSCRFLAMTLRADEVESSLNSESSSHVRRGDLFIAFNAADLSESVILPQPPEG 834

Query: 2316 YAWVCLVDTALPYPGFFS-----MDSNFCDGLSTYELKPHSCTLFEAGMS 2450
             +W CLVDTALP+PGFFS     +     + ++ YE+K HSC LFEA  S
Sbjct: 835  MSWRCLVDTALPFPGFFSTSGETLPEQMAESVA-YEMKSHSCALFEASSS 883


>ref|XP_011624242.1| isoamylase 2, chloroplastic isoform X2 [Amborella trichopoda]
          Length = 891

 Score =  951 bits (2457), Expect = 0.0
 Identities = 470/826 (56%), Positives = 597/826 (72%), Gaps = 13/826 (1%)
 Frame = +3

Query: 3    KLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVV 182
            K+ + K+   S NS   I+  L        ++   +++FRTE+GG VKV+V   +M YV+
Sbjct: 66   KINDGKLFAISPNS---IEIALDKRIQTNVIEDRTTFMFRTEIGGQVKVVVSQKSMNYVI 122

Query: 183  NIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQ 362
            ++E+ SL     ++ L L WG+FRSDSS     DS+ S   T    S+ G   ++T F +
Sbjct: 123  SVELISLTDVCYQN-LELHWGIFRSDSSCWVLLDSENSPSGTDLVKSESGEDCMKTLFKK 181

Query: 363  NSLGTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPL 542
               G H++ L FDS Q P                +S I+THRKTNF VPVGI  G P+PL
Sbjct: 182  TCSGEHSLELMFDSCQAPFYISFIVYSPFDSAWGNSQIRTHRKTNFVVPVGIGRGNPLPL 241

Query: 543  GFSKSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGN 722
            G S ++DG  NF+LFSR AE+V LCLY+    +P+L+IELDPYINR+GD+WHVS+ SV  
Sbjct: 242  GVSLNDDGSTNFSLFSRNAENVVLCLYDENTAKPALEIELDPYINRSGDMWHVSLSSVRQ 301

Query: 723  YVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESV----YLGYLGDEPTF 890
            Y+SYG+RCKG I WD G+R+H   +LLDPYAK++ NF  ++G SV     LG L  E  F
Sbjct: 302  YLSYGFRCKGAILWDKGNRYHMRRILLDPYAKILGNFNYNEGGSVPLVKCLGRLYTETAF 361

Query: 891  DWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVL 1070
            DW GD  P +PMEKL+VYRLNVG FT+D SS LP+++AGTF GVI+KL H K+LGVNA+L
Sbjct: 362  DWDGDTSPCIPMEKLMVYRLNVGRFTEDMSSLLPKDIAGTFLGVIQKLHHLKNLGVNALL 421

Query: 1071 LEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFT 1250
            LEPIFP+DE +GPY+PY+FF+PMN YG  RDG+S   SMKEMVKALHA+G+EVLLE+VFT
Sbjct: 422  LEPIFPFDELKGPYYPYNFFAPMNKYGPLRDGISACTSMKEMVKALHANGLEVLLEMVFT 481

Query: 1251 HTGEGGTA-CQTISFRGIDNSSYYITEGGM----GSGMNCNNPIVQQLILDSLHYWLTEF 1415
            HT EGG + CQTISFRGIDNSSYYI +  +    G+ +NCN+P+VQ +ILD L +W+ E+
Sbjct: 482  HTAEGGDSLCQTISFRGIDNSSYYIVDRNVESEGGNVLNCNHPMVQTMILDCLRHWVHEY 541

Query: 1416 HVDGFCFINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFP 1595
            HVDGFCFINSS L +GS+G+ L+  PL+EAIAFDP+LS  KIIADCW P+D    +IHFP
Sbjct: 542  HVDGFCFINSSSLAKGSDGELLTLSPLIEAIAFDPILSHAKIIADCWSPLDMQCKEIHFP 601

Query: 1596 HWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLP 1775
            HW++WAE+NARFC DVRNFLRGEGLLS+LATRLCGSGD+FSD RGP+FSFNY+ +NFGLP
Sbjct: 602  HWKKWAEMNARFCYDVRNFLRGEGLLSNLATRLCGSGDIFSDGRGPSFSFNYIARNFGLP 661

Query: 1776 LVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDE 1955
            LVDLVSFS +EL++ELSWNCGEEG T+  +VLE RLKQIRNFLFIL+ISLG+PV NMGDE
Sbjct: 662  LVDLVSFSGSELSAELSWNCGEEGPTSTPVVLESRLKQIRNFLFILYISLGVPVLNMGDE 721

Query: 1956 CGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNID 2135
             G STGG   Y  R+  DW  L+T +G Q TQ++ FLSSLR +RSDL Q + F+K++++D
Sbjct: 722  YGQSTGGSTLYSNRKSFDWGSLRTDFGVQTTQYVTFLSSLRTKRSDLLQRKHFMKIEHLD 781

Query: 2136 WHGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEG 2315
            WHG +QS P+WE PS KFLA+T+    +     S+ GDL+   N     E  +LPQ+   
Sbjct: 782  WHGEDQSQPQWEAPSSKFLAVTVNTGDDETETRSNGGDLYFAINAHGSSECAVLPQVSNN 841

Query: 2316 YAWVCLVDTALPYPGFFSMDSNFCD----GLSTYELKPHSCTLFEA 2441
             AW CLVDT+LPYPGFFSM+    D     ++ Y +KPHSCTLFEA
Sbjct: 842  MAWFCLVDTSLPYPGFFSMEGIPIDQPATSIAIYSMKPHSCTLFEA 887


>gb|ERN08315.1| hypothetical protein AMTR_s00156p00067780 [Amborella trichopoda]
          Length = 926

 Score =  951 bits (2457), Expect = 0.0
 Identities = 470/826 (56%), Positives = 597/826 (72%), Gaps = 13/826 (1%)
 Frame = +3

Query: 3    KLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVV 182
            K+ + K+   S NS   I+  L        ++   +++FRTE+GG VKV+V   +M YV+
Sbjct: 101  KINDGKLFAISPNS---IEIALDKRIQTNVIEDRTTFMFRTEIGGQVKVVVSQKSMNYVI 157

Query: 183  NIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQ 362
            ++E+ SL     ++ L L WG+FRSDSS     DS+ S   T    S+ G   ++T F +
Sbjct: 158  SVELISLTDVCYQN-LELHWGIFRSDSSCWVLLDSENSPSGTDLVKSESGEDCMKTLFKK 216

Query: 363  NSLGTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPL 542
               G H++ L FDS Q P                +S I+THRKTNF VPVGI  G P+PL
Sbjct: 217  TCSGEHSLELMFDSCQAPFYISFIVYSPFDSAWGNSQIRTHRKTNFVVPVGIGRGNPLPL 276

Query: 543  GFSKSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGN 722
            G S ++DG  NF+LFSR AE+V LCLY+    +P+L+IELDPYINR+GD+WHVS+ SV  
Sbjct: 277  GVSLNDDGSTNFSLFSRNAENVVLCLYDENTAKPALEIELDPYINRSGDMWHVSLSSVRQ 336

Query: 723  YVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESV----YLGYLGDEPTF 890
            Y+SYG+RCKG I WD G+R+H   +LLDPYAK++ NF  ++G SV     LG L  E  F
Sbjct: 337  YLSYGFRCKGAILWDKGNRYHMRRILLDPYAKILGNFNYNEGGSVPLVKCLGRLYTETAF 396

Query: 891  DWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVL 1070
            DW GD  P +PMEKL+VYRLNVG FT+D SS LP+++AGTF GVI+KL H K+LGVNA+L
Sbjct: 397  DWDGDTSPCIPMEKLMVYRLNVGRFTEDMSSLLPKDIAGTFLGVIQKLHHLKNLGVNALL 456

Query: 1071 LEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFT 1250
            LEPIFP+DE +GPY+PY+FF+PMN YG  RDG+S   SMKEMVKALHA+G+EVLLE+VFT
Sbjct: 457  LEPIFPFDELKGPYYPYNFFAPMNKYGPLRDGISACTSMKEMVKALHANGLEVLLEMVFT 516

Query: 1251 HTGEGGTA-CQTISFRGIDNSSYYITEGGM----GSGMNCNNPIVQQLILDSLHYWLTEF 1415
            HT EGG + CQTISFRGIDNSSYYI +  +    G+ +NCN+P+VQ +ILD L +W+ E+
Sbjct: 517  HTAEGGDSLCQTISFRGIDNSSYYIVDRNVESEGGNVLNCNHPMVQTMILDCLRHWVHEY 576

Query: 1416 HVDGFCFINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFP 1595
            HVDGFCFINSS L +GS+G+ L+  PL+EAIAFDP+LS  KIIADCW P+D    +IHFP
Sbjct: 577  HVDGFCFINSSSLAKGSDGELLTLSPLIEAIAFDPILSHAKIIADCWSPLDMQCKEIHFP 636

Query: 1596 HWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLP 1775
            HW++WAE+NARFC DVRNFLRGEGLLS+LATRLCGSGD+FSD RGP+FSFNY+ +NFGLP
Sbjct: 637  HWKKWAEMNARFCYDVRNFLRGEGLLSNLATRLCGSGDIFSDGRGPSFSFNYIARNFGLP 696

Query: 1776 LVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDE 1955
            LVDLVSFS +EL++ELSWNCGEEG T+  +VLE RLKQIRNFLFIL+ISLG+PV NMGDE
Sbjct: 697  LVDLVSFSGSELSAELSWNCGEEGPTSTPVVLESRLKQIRNFLFILYISLGVPVLNMGDE 756

Query: 1956 CGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNID 2135
             G STGG   Y  R+  DW  L+T +G Q TQ++ FLSSLR +RSDL Q + F+K++++D
Sbjct: 757  YGQSTGGSTLYSNRKSFDWGSLRTDFGVQTTQYVTFLSSLRTKRSDLLQRKHFMKIEHLD 816

Query: 2136 WHGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEG 2315
            WHG +QS P+WE PS KFLA+T+    +     S+ GDL+   N     E  +LPQ+   
Sbjct: 817  WHGEDQSQPQWEAPSSKFLAVTVNTGDDETETRSNGGDLYFAINAHGSSECAVLPQVSNN 876

Query: 2316 YAWVCLVDTALPYPGFFSMDSNFCD----GLSTYELKPHSCTLFEA 2441
             AW CLVDT+LPYPGFFSM+    D     ++ Y +KPHSCTLFEA
Sbjct: 877  MAWFCLVDTSLPYPGFFSMEGIPIDQPATSIAIYSMKPHSCTLFEA 922


>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
          Length = 866

 Score =  942 bits (2435), Expect = 0.0
 Identities = 487/799 (60%), Positives = 582/799 (72%), Gaps = 14/799 (1%)
 Frame = +3

Query: 87   EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSS 266
            +K ++  +YLFRTE+GG VKVLV   N+KY V +EVSSL L   E  L+++WG+FRSDSS
Sbjct: 80   QKEEKSSAYLFRTEIGGHVKVLVRQKNLKYSVYVEVSSLKLKSGEDKLVMNWGIFRSDSS 139

Query: 267  HLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXX 446
                 D Q+S  D   ST       + TPF QNSLG   I L F+ +  P          
Sbjct: 140  QFIPLDLQSSTPDDRSST-------VETPFVQNSLGKLVIELNFEGSLTPFYLSFLLKFP 192

Query: 447  XXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSEDGLVNFALFSRKAESVNLCLYE 626
                +  S +++HRKT+FCVPVG++SG P PLG S S DG +NFALFS+ A+ V LCLY+
Sbjct: 193  SDGDSKSSDLRSHRKTSFCVPVGLASGYPAPLGLSFSADGSINFALFSQSADGVVLCLYD 252

Query: 627  -SGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLL 803
             + A +P+L+I+LDPY+NR+GDIWHVSM+S   +VSYGYRCKG I    G + H+ HVLL
Sbjct: 253  DTKAEKPTLEIDLDPYVNRSGDIWHVSMDSALPFVSYGYRCKGGIH-GKGVKDHSEHVLL 311

Query: 804  DPYAKLIKNFIPDQGESVY--LGYLGDEPTFDWSGDVHPQLPMEKLVVYRLNVGSFTKDK 977
            DPYAK+I N       SV   LG L  EPTFDWSGD+ P +PMEKLVVYR+NV  FTK K
Sbjct: 312  DPYAKVIANA---HSGSVLKTLGKLIKEPTFDWSGDIRPCIPMEKLVVYRVNVAHFTKHK 368

Query: 978  SSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFPYDEKRGPYFPYHFFSPMNSYGCT 1157
            SS LP +VAG+F G+ EKLQH K LGVNA+LLEP+FP+DEK+GPYFP+HFFSPM  YG +
Sbjct: 369  SSGLPNDVAGSFTGITEKLQHCKDLGVNAILLEPVFPFDEKKGPYFPFHFFSPMKLYGPS 428

Query: 1158 RDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEGGTACQTISFRGIDNSSYYIT---E 1328
             D  +TV SMKEMVK LHA+GIEVLLE+VFTHT E      + S R ID SSYYIT   +
Sbjct: 429  DDPSATVNSMKEMVKILHANGIEVLLEIVFTHTAE------SCSLREIDKSSYYITGDED 482

Query: 1329 GGMGSGMNCNNPIVQQLILDSLHYWLTEFHVDGFCFINSSYLLQGSNGDRLSRPPLVEAI 1508
             G  + +NCN PIVQQ+ILDSL YW  EFHVDGFCFIN+S LL+G +G+ LSRPPLVEAI
Sbjct: 483  LGAKNALNCNYPIVQQMILDSLRYWAIEFHVDGFCFINASSLLKGFHGEFLSRPPLVEAI 542

Query: 1509 AFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRWAEINARFCRDVRNFLRGEGLLSDLAT 1688
            AFDPLLSKTK+IAD W P D +  +I FPHW+RWAE+N  FC DVRNFLRGEGLLS+LAT
Sbjct: 543  AFDPLLSKTKLIADSWDPHDMASDEILFPHWKRWAEVNTNFCGDVRNFLRGEGLLSNLAT 602

Query: 1689 RLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVSFSSNELASELSWNCGEEGATNKKLV 1868
            RLCGSGD+F + RGP FSFNY+T+N GLPLVDLVSFSS+ LASELSWNCGEEG TN   V
Sbjct: 603  RLCGSGDMFLNGRGPGFSFNYITRNSGLPLVDLVSFSSSNLASELSWNCGEEGPTNNTAV 662

Query: 1869 LEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTGGLFAYDGRQPIDWNGLKTVYGKQIT 2048
            LE RLKQIRN+LFILF+S G+PV NMGDECG S+GG  AY  R+P DWN L+T +G Q T
Sbjct: 663  LERRLKQIRNYLFILFVSFGVPVLNMGDECGQSSGGSPAYGDRKPYDWNALRTGFGIQTT 722

Query: 2049 QFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQSDPKWEDPSCKFLAMTLRAEKNGKL 2228
            QFI+FLSSLR+RRSDL Q R FLK +NIDW+GS+QS P W+D S +FLAMTL+ E+    
Sbjct: 723  QFISFLSSLRVRRSDLLQKRSFLKEENIDWYGSDQSLPTWDDRSSRFLAMTLKVEREENE 782

Query: 2229 PSSD----KGDLFVCFNGSDLPESVILPQLPEGYAWVCLVDTALPYPGFFSMDS----NF 2384
             SS+     GDLFV FN +D  E VILP  P    W+ LVDTA+P+PGFFS D       
Sbjct: 783  SSSEFSSLNGDLFVAFNSADQSERVILPPPPPEMVWLRLVDTAVPFPGFFSRDGEPVLEQ 842

Query: 2385 CDGLSTYELKPHSCTLFEA 2441
              GL TYE+K HSC LFEA
Sbjct: 843  MAGLVTYEMKSHSCVLFEA 861


>ref|XP_018858427.1| PREDICTED: isoamylase 2, chloroplastic [Juglans regia]
          Length = 889

 Score =  939 bits (2427), Expect = 0.0
 Identities = 482/827 (58%), Positives = 589/827 (71%), Gaps = 16/827 (1%)
 Frame = +3

Query: 12   NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 191
            N K+   S    ++ +  +      E + +  +YLF TE+GG VKV V   N+KY V +E
Sbjct: 64   NSKVYATSRVFIKETEQRVTTITEVEDMLKSLTYLFWTEIGGQVKVSVRKKNVKYAVYVE 123

Query: 192  VSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIR-TPFTQNS 368
            VSSL L+ ++  L+LSWG++R DSS     D+Q+S  D        GRA+ R TPF QN+
Sbjct: 124  VSSLQLHGSDDRLLLSWGIYRDDSSCFMHLDAQSSTPD--------GRATTRETPFIQNT 175

Query: 369  LGTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGF 548
             G   + L+F+  ++P               + S I++HRKTNFCVPVG  SG P PLG 
Sbjct: 176  KGRFVLELDFEEKKIPFYLSFLLKSSLGSDPSGSEIRSHRKTNFCVPVGFGSGYPAPLGL 235

Query: 549  SKSEDGLVNFALFSRKAESVNLCLYESGA-NQPSLDIELDPYINRTGDIWHVSMESVGNY 725
            + S DG +NF++FSR AESV LCLY+    ++P+L+++LDPY NR+GD+WH S+ES    
Sbjct: 236  TFSPDGSMNFSIFSRNAESVVLCLYDDMTIDEPTLELDLDPYTNRSGDVWHASLESAWTS 295

Query: 726  VSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVYLGYLGDEPTFDWSGD 905
            VSYGYRCKG +T  N     A  +LLDPYAK+I N IP    S YLG L  EP FDW  D
Sbjct: 296  VSYGYRCKGALTQRNKVNADAGEILLDPYAKIIGNSIPSNRGSGYLGRLCKEPAFDWGDD 355

Query: 906  VHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIF 1085
            VHP LPMEKL VYRLNV  FT+ KSS+LP++VAGTF+G+ EKLQHFK L +NAVLLEPIF
Sbjct: 356  VHPNLPMEKLAVYRLNVRHFTEHKSSQLPKDVAGTFSGLTEKLQHFKDLSMNAVLLEPIF 415

Query: 1086 PYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEG 1265
             + E+ GPYFP HFFSP N YG + D MST+ SMKEMVK LHA+GIEVLLEV FTHT  G
Sbjct: 416  SFHEQNGPYFPCHFFSPTNQYGPSGDSMSTINSMKEMVKKLHANGIEVLLEVAFTHTAVG 475

Query: 1266 GTACQTISFRGIDNSSYYITEGGMGS----GMNCNNPIVQQLILDSLHYWLTEFHVDGFC 1433
            G      + +GID+ SYY + G   S     +NCN PIVQQLILDSL YW+TEFHVDGFC
Sbjct: 476  G------ALQGIDDLSYYYSNGVGDSEATNSLNCNYPIVQQLILDSLRYWVTEFHVDGFC 529

Query: 1434 FINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRWA 1613
            FIN+S L++G +G+ LSRPPLVEAIAFDP LSK KIIADCW P D    +  FPHW++WA
Sbjct: 530  FINASSLMRGFHGEHLSRPPLVEAIAFDPFLSKIKIIADCWDPHDMLPKETRFPHWKKWA 589

Query: 1614 EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 1793
            EIN +FC DVRNF RGEGLLS LATRLCGSGD FSD RGP+FSFN+  ++FGL LVDLVS
Sbjct: 590  EINTKFCNDVRNFWRGEGLLSSLATRLCGSGDTFSDGRGPSFSFNFTARSFGLTLVDLVS 649

Query: 1794 FSSNE-LASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFST 1970
            FS+ + LAS LSWNCGEEG T+   VLE+RLKQIRNFLF+L+ISLG+P+ NMGDECG S+
Sbjct: 650  FSNTDALASHLSWNCGEEGPTDNTTVLEMRLKQIRNFLFVLYISLGVPILNMGDECGQSS 709

Query: 1971 GGLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSN 2150
            GG  AY  RQP DWN L+T +G Q TQFI+FL+SLR RRSDL Q R FLK +NIDWHG++
Sbjct: 710  GGSLAYVDRQPFDWNALRTGFGIQTTQFISFLNSLRTRRSDLLQKRSFLKEENIDWHGND 769

Query: 2151 QSDPKWEDPSCKFLAMTLRAEKNGKLP-----SSDKGDLFVCFNGSDLPESVILPQLPEG 2315
            QS P WEDPSCKFLA+TL+A+K  K P     S  +GDLF+ FN +D  ESVILP+ PEG
Sbjct: 770  QSPPSWEDPSCKFLAVTLKADK-VKCPLNSESSHLRGDLFIAFNAADHSESVILPEPPEG 828

Query: 2316 YAWVCLVDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEAG 2444
             +W  LVDTALP+PGFFS++        +GL+ YE+K HS  LFEAG
Sbjct: 829  MSWRRLVDTALPFPGFFSINGEPIPEQMEGLAAYEMKSHSSALFEAG 875


>gb|OMO77794.1| hypothetical protein CCACVL1_14832 [Corchorus capsularis]
          Length = 878

 Score =  938 bits (2425), Expect = 0.0
 Identities = 474/825 (57%), Positives = 592/825 (71%), Gaps = 17/825 (2%)
 Frame = +3

Query: 30   ASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPL 209
            AS  S EQ +         ++L+++ +Y+FRTE+GG VK+ V   ++KYVV+IEVSSL L
Sbjct: 74   ASRVSVEQTEQTFTSTSQVDELKKLSTYMFRTEIGGQVKIFVRKKSVKYVVDIEVSSLQL 133

Query: 210  YLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAIT 389
              + + L+LS G++RSD                     DV   +I TPF   S    A+ 
Sbjct: 134  SGDNNKLVLSGGVYRSDH--------------------DVKTNNIETPFIARSSSELALE 173

Query: 390  LEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSEDGL 569
            LEF++ + P              ++   I++HRKTNFCVP+G   G P+PLG S S DG 
Sbjct: 174  LEFEAKEAPFYFSFLLKASSDANSSGLEIRSHRKTNFCVPIGFDQGYPVPLGLSFSNDGS 233

Query: 570  VNFALFSRKAESVNLCLYE-SGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRC 746
            +NFA++SR AES+ LCLY+ +G+ +P+L+++LDPY+NRTGDIWH S+E    +VSYGYRC
Sbjct: 234  MNFAVYSRNAESLVLCLYDDTGSEKPALELDLDPYVNRTGDIWHASLEGAWTFVSYGYRC 293

Query: 747  KGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGES----VYLGYLGDEPTFDWSGDVHP 914
            KG+     G  FHA  VLLDPYAK+I + IP+  ES     +LG L  EP FDWSGDV P
Sbjct: 294  KGD-----GDAFHAERVLLDPYAKIIGSSIPNHYESGLLLKHLGRLCKEPAFDWSGDVCP 348

Query: 915  QLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFPYD 1094
             LP+EKLVVYRLNV  FT+DKSS+LP NVAGTF+GV EK+QH K LG+NAVLLEPIF +D
Sbjct: 349  NLPLEKLVVYRLNVMHFTEDKSSKLPANVAGTFSGVTEKVQHLKYLGINAVLLEPIFTFD 408

Query: 1095 EKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEGGTA 1274
            E++GPYFP HFFSP + YG +   +S + S+K MVK LHA+GIEVLLEVVFTHT EGG  
Sbjct: 409  EQKGPYFPCHFFSPTSLYGPSNGSISAINSIKGMVKNLHANGIEVLLEVVFTHTAEGG-- 466

Query: 1275 CQTISFRGIDNSSYY----ITEGGMGSGMNCNNPIVQQLILDSLHYWLTEFHVDGFCFIN 1442
                + +G+D+ SYY    + +    + +NCN P+VQQ+ILDSL +W+TEFH+DGFCFIN
Sbjct: 467  ----ALQGLDDESYYYRNRVEDLEEKNALNCNYPVVQQMILDSLRHWVTEFHIDGFCFIN 522

Query: 1443 SSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRWAEIN 1622
            +S LL+G +G+ LSRPPLVEAIAFDPLLSKTK+IADCW P +    +IHFPHW+RWAE+N
Sbjct: 523  ASCLLRGFHGEHLSRPPLVEAIAFDPLLSKTKVIADCWDPHEMMPKEIHFPHWKRWAEMN 582

Query: 1623 ARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVSFSS 1802
             +FC D+RNFLRGE  LS LATRLCGSGD+FSD RGP FSFN++ +NFGLPLVDLVSFS+
Sbjct: 583  TKFCSDIRNFLRGEDALSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFSN 642

Query: 1803 NELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTGGLF 1982
             ELASELSWNCGEEG T+   VLE RLKQIRN++FILF+SLG+PV NMGDECG S+GG  
Sbjct: 643  AELASELSWNCGEEGPTSNTAVLERRLKQIRNYIFILFVSLGVPVLNMGDECGQSSGGSL 702

Query: 1983 AYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQSDP 2162
            +Y  R+ +DWN + T +G Q TQFI+FLSSLR+RRSDL Q R+FLK +NI+WHGS+QS P
Sbjct: 703  SYGSRKLLDWNAMTTGFGIQTTQFISFLSSLRVRRSDLLQRRNFLKEENIEWHGSSQSPP 762

Query: 2163 KWEDPSCKFLAMTLRAEKNGKLPSSD----KGDLFVCFNGSDLPESVILPQLPEGYAWVC 2330
            +WEDPSCKFLAMTL+A+K   L SS+    KGDLF+  N  D  E++ILP  PEG AW  
Sbjct: 763  EWEDPSCKFLAMTLKADKAESLLSSEASELKGDLFIAINADDKTENIILPPCPEGMAWRR 822

Query: 2331 LVDTALPYPGFFSMDSNF----CDGLSTYELKPHSCTLFEAGMSD 2453
            LVDTALPYPGFF  D         GL  YE+K  SCTLFEA   D
Sbjct: 823  LVDTALPYPGFFLADGKAVLEQMAGLVAYEMKSRSCTLFEACTED 867


>ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]
 gb|ONH93660.1| hypothetical protein PRUPE_8G244800 [Prunus persica]
          Length = 883

 Score =  936 bits (2418), Expect = 0.0
 Identities = 480/826 (58%), Positives = 587/826 (71%), Gaps = 16/826 (1%)
 Frame = +3

Query: 12   NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 191
            N+K+   S  S E ++         E++ +V +YLFRTE+G +V V V   N KY VNIE
Sbjct: 68   NLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFVRKRNAKYTVNIE 127

Query: 192  VSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 371
            V S  L  N+  L+L WGM+R+DSS  + P      LD   ST +    ++ TP  Q S 
Sbjct: 128  VPSSHLSSNDRRLVLRWGMYRADSS-CFVP------LDFKSSTPNDTTTTLETPLIQTSS 180

Query: 372  GTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 551
            G   + LEF++ Q+P               +D  I++HRKTNFCVPVG   G P PLG +
Sbjct: 181  GRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLT 240

Query: 552  KSEDGLVNFALFSRKAESVNLCLYESG-ANQPSLDIELDPYINRTGDIWHVSMESVGNYV 728
             S DG +NFA+FSR AESV LCLY++  A +P+L+++LDPY+NR+GDIWHVS  S  ++V
Sbjct: 241  FSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWSFV 300

Query: 729  SYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGES--VYLGYLGDEPTFDWSG 902
            SYGY+ KG +   N + F   HVLLDPYAK+I   IP+   +   YLG L +EP FDW+G
Sbjct: 301  SYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGLKYLGRLCEEPAFDWAG 360

Query: 903  DVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPI 1082
            DV P L MEKLVVYRLNV  FT+ KSS+LP N+ G+F+G+ EKL+HFK LGVNAVLLEPI
Sbjct: 361  DVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGVNAVLLEPI 420

Query: 1083 FPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGE 1262
            FP+DE++GPYFP+HFFSPM+ +G +R  +S V SMKEMV+  HA+GIEVLLEVVFTHT E
Sbjct: 421  FPFDEQKGPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLLEVVFTHTAE 480

Query: 1263 GGTACQTISFRGIDNSSYY----ITEGGMGSGMNCNNPIVQQLILDSLHYWLTEFHVDGF 1430
            G       + +GID SSYY    + +    + +NCN PIVQQL+LDSL YW+TEFHVDGF
Sbjct: 481  GE------ALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHVDGF 534

Query: 1431 CFINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRW 1610
             FIN+S LL+G NG+ LSRPPLVEAIAFDPLLSKTKIIADCW P   +  + HFPHWRRW
Sbjct: 535  FFINASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETHFPHWRRW 594

Query: 1611 AEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLV 1790
            AE+N +FC DVRNFLRGEGLLSDLATRLCG+GD+FSD RGP F+FN++++N GLPLVDLV
Sbjct: 595  AEVNTKFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLV 654

Query: 1791 SFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFST 1970
            SFS  ELASELSWNCGEEG TNK  VLE RLKQIRNFLFILF+SLG+PV NMGDECG ST
Sbjct: 655  SFSGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQST 714

Query: 1971 GGLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSN 2150
            GG  AY  R+  DWN L+T +  Q TQFIAFLSS R RRSDL Q R+FLK +NI W+ S+
Sbjct: 715  GGSPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIGWYESD 774

Query: 2151 QSDPKWEDPSCKFLAMTLRAEKN-----GKLPSSDKGDLFVCFNGSDLPESVILPQLPEG 2315
            Q+ P+WEDPS KFLAM L+A+++     G   S   GDLFV F+ +D  E+V+LP   EG
Sbjct: 775  QTPPRWEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHSETVVLPPPLEG 834

Query: 2316 YAWVCLVDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEA 2441
              W  LVDTALP+PGFFS D         GL  YE+K HSC LFEA
Sbjct: 835  MGWRRLVDTALPFPGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEA 880


>ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium]
          Length = 883

 Score =  935 bits (2417), Expect = 0.0
 Identities = 478/826 (57%), Positives = 588/826 (71%), Gaps = 16/826 (1%)
 Frame = +3

Query: 12   NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 191
            N+K+   S  S E ++         E++ +V +YLFRTE+G +V V V   N KY VNIE
Sbjct: 68   NLKVYAKSRVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFVRKRNAKYTVNIE 127

Query: 192  VSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 371
            V SL L  ++  L+L WG++R+DSS  + P      LD   ST +    ++ TPFTQ S 
Sbjct: 128  VPSLYLSSSDRRLVLRWGIYRADSS-CFVP------LDFKSSTPNDTTTTLETPFTQTSS 180

Query: 372  GTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 551
            G     LEF++ Q+P               +D  I++HRKTNFCVPVG   G P PLG +
Sbjct: 181  GRFTFELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLT 240

Query: 552  KSEDGLVNFALFSRKAESVNLCLYE-SGANQPSLDIELDPYINRTGDIWHVSMESVGNYV 728
             S DG +NFA+FSR AESV LCLY+ + A +P+L+++LDPY+NR+GDIWHVS  S   +V
Sbjct: 241  FSNDGSMNFAIFSRNAESVVLCLYDDTTAEKPALELDLDPYVNRSGDIWHVSFGSARAFV 300

Query: 729  SYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGES--VYLGYLGDEPTFDWSG 902
            SYGY+ KG +   N + F   HVLLDPYAK+I   IP+   +   YLG L +EP FDW+G
Sbjct: 301  SYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGLKYLGRLCEEPAFDWAG 360

Query: 903  DVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPI 1082
            DV P L MEKLVVYRLNV  FT+ KSS+LP ++ G+F+G+ EKL+HFK LGVNAVLLEPI
Sbjct: 361  DVRPDLSMEKLVVYRLNVMRFTEHKSSQLPTDIGGSFSGLTEKLEHFKDLGVNAVLLEPI 420

Query: 1083 FPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGE 1262
            FP+DE++GPYFP HFFSPM+ +G +   +S V SMKEMV+  HA+GIEVLLEVVFTHT E
Sbjct: 421  FPFDEQKGPYFPRHFFSPMDCFGPSGGPVSAVNSMKEMVRKFHANGIEVLLEVVFTHTAE 480

Query: 1263 GGTACQTISFRGIDNSSYY----ITEGGMGSGMNCNNPIVQQLILDSLHYWLTEFHVDGF 1430
            G       + +GID SSYY    + +    + +NCN P+VQQL+LDSL YW+TEFHVDGF
Sbjct: 481  GE------ALQGIDISSYYHVNEVEDLEARNALNCNYPVVQQLVLDSLRYWVTEFHVDGF 534

Query: 1431 CFINSSYLLQGSNGDRLSRPPLVEAIAFDPLLSKTKIIADCWCPVDTSHSDIHFPHWRRW 1610
            CFIN+S LL+G NG+ LSRPPLVEAIAFDPLLSKTKIIAD W P   +  +  FPHW+RW
Sbjct: 535  CFINASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADRWDPHGMAPKETRFPHWKRW 594

Query: 1611 AEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLV 1790
            AE+N +FC DVRNFLRGEGLLSDLATRLCG+GD+FSD RGP F+FN++++N GLPLVDLV
Sbjct: 595  AEVNTKFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLV 654

Query: 1791 SFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFST 1970
            SFS  ELASELSWNCGEEG TNK  VLE RLKQIRNFLFILF+SLG+PV NMGDECG ST
Sbjct: 655  SFSGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQST 714

Query: 1971 GGLFAYDGRQPIDWNGLKTVYGKQITQFIAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSN 2150
            GG  AY  R+  DWN L+T +  Q TQFIAFLSS R RRSDL Q R+FLK +NI W+ S+
Sbjct: 715  GGSPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRNRRSDLLQKRNFLKEENIYWYESD 774

Query: 2151 QSDPKWEDPSCKFLAMTLRAEKN-----GKLPSSDKGDLFVCFNGSDLPESVILPQLPEG 2315
            Q+ P+WEDPSCKFLAM L+A+++     G   S  +GDLFV F+ +D  E+V+LP   EG
Sbjct: 775  QTPPRWEDPSCKFLAMRLKADEDEVNQPGDESSRSRGDLFVAFSAADHSETVVLPPPLEG 834

Query: 2316 YAWVCLVDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEA 2441
              W CLVDTALP+PGFFS D         GL  YE+K HSC LFEA
Sbjct: 835  MGWRCLVDTALPFPGFFSTDGEPVVEQMVGLFAYEMKSHSCALFEA 880