BLASTX nr result
ID: Ophiopogon26_contig00041654
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon26_contig00041654
(545 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EXX79829.1| Tip41p [Rhizophagus irregularis DAOM 197198w] 242 1e-79
gb|PKY16507.1| TIP41-like protein [Rhizophagus irregularis] 242 4e-78
gb|PKY42093.1| TIP41-domain-containing protein [Rhizophagus irre... 242 1e-77
gb|EXX63022.1| Tip41p [Rhizophagus irregularis DAOM 197198w] >gi... 242 1e-77
gb|PKK72648.1| TIP41-domain-containing protein [Rhizophagus irre... 242 2e-77
gb|ORX92582.1| TIP41-domain-containing protein [Basidiobolus mer... 136 4e-36
gb|KFH69603.1| hypothetical protein MVEG_04409 [Mortierella vert... 129 1e-33
ref|XP_018297796.1| hypothetical protein PHYBLDRAFT_179237 [Phyc... 128 3e-33
ref|XP_016609180.1| hypothetical protein SPPG_04232, partial [Sp... 127 2e-32
ref|XP_021881223.1| TIP41-like family-domain-containing protein ... 126 3e-32
gb|EPB87275.1| hypothetical protein HMPREF1544_05896 [Mucor circ... 125 3e-32
gb|ORZ10766.1| TIP41-like family-domain-containing protein [Absi... 125 4e-32
gb|OON07213.1| hypothetical protein, variant [Batrachochytrium s... 124 5e-32
gb|OON07212.1| hypothetical protein BSLG_03270 [Batrachochytrium... 124 2e-31
gb|OAQ24169.1| TIP41-domain-containing protein [Mortierella elon... 123 3e-31
emb|CDH52482.1| type 2a phosphatase activator tip41 [Lichtheimia... 122 9e-31
emb|CDS05921.1| hypothetical protein LRAMOSA08449 [Lichtheimia r... 121 1e-30
emb|SAM05970.1| hypothetical protein [Absidia glauca] 123 2e-30
emb|CEP12211.1| hypothetical protein [Parasitella parasitica] 120 4e-30
gb|EIE84533.1| hypothetical protein RO3G_09243 [Rhizopus delemar... 120 5e-30
>gb|EXX79829.1| Tip41p [Rhizophagus irregularis DAOM 197198w]
Length = 133
Score = 242 bits (617), Expect = 1e-79
Identities = 116/119 (97%), Positives = 118/119 (99%)
Frame = -1
Query: 545 EIDLFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 366
EIDLFEDELADNGTAI+NVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ
Sbjct: 15 EIDLFEDELADNGTAIINVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 74
Query: 365 LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLPPPNKDELMVKKLCVVPKSEI 189
LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWIN+KLPPP KDELMVKKLCVVPKSEI
Sbjct: 75 LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWINSKLPPPKKDELMVKKLCVVPKSEI 133
>gb|PKY16507.1| TIP41-like protein [Rhizophagus irregularis]
Length = 239
Score = 242 bits (617), Expect = 4e-78
Identities = 116/119 (97%), Positives = 118/119 (99%)
Frame = -1
Query: 545 EIDLFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 366
EIDLFEDELADNGTAI+NVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ
Sbjct: 121 EIDLFEDELADNGTAIINVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 180
Query: 365 LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLPPPNKDELMVKKLCVVPKSEI 189
LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWIN+KLPPP KDELMVKKLCVVPKSEI
Sbjct: 181 LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWINSKLPPPKKDELMVKKLCVVPKSEI 239
>gb|PKY42093.1| TIP41-domain-containing protein [Rhizophagus irregularis]
Length = 278
Score = 242 bits (617), Expect = 1e-77
Identities = 116/119 (97%), Positives = 118/119 (99%)
Frame = -1
Query: 545 EIDLFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 366
EIDLFEDELADNGTAI+NVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ
Sbjct: 160 EIDLFEDELADNGTAIINVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 219
Query: 365 LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLPPPNKDELMVKKLCVVPKSEI 189
LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWIN+KLPPP KDELMVKKLCVVPKSEI
Sbjct: 220 LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWINSKLPPPKKDELMVKKLCVVPKSEI 278
>gb|EXX63022.1| Tip41p [Rhizophagus irregularis DAOM 197198w]
dbj|GBC47499.1| Type 2A phosphatase activator TIP41 [Rhizophagus irregularis DAOM
181602]
gb|PKC14616.1| TIP41-domain-containing protein [Rhizophagus irregularis]
gb|PKC74671.1| TIP41-domain-containing protein [Rhizophagus irregularis]
gb|POG74583.1| hypothetical protein GLOIN_2v1575114 [Rhizophagus irregularis DAOM
181602=DAOM 197198]
Length = 278
Score = 242 bits (617), Expect = 1e-77
Identities = 116/119 (97%), Positives = 118/119 (99%)
Frame = -1
Query: 545 EIDLFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 366
EIDLFEDELADNGTAI+NVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ
Sbjct: 160 EIDLFEDELADNGTAIINVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 219
Query: 365 LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLPPPNKDELMVKKLCVVPKSEI 189
LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWIN+KLPPP KDELMVKKLCVVPKSEI
Sbjct: 220 LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWINSKLPPPKKDELMVKKLCVVPKSEI 278
>gb|PKK72648.1| TIP41-domain-containing protein [Rhizophagus irregularis]
Length = 290
Score = 242 bits (617), Expect = 2e-77
Identities = 116/119 (97%), Positives = 118/119 (99%)
Frame = -1
Query: 545 EIDLFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 366
EIDLFEDELADNGTAI+NVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ
Sbjct: 172 EIDLFEDELADNGTAIINVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 231
Query: 365 LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLPPPNKDELMVKKLCVVPKSEI 189
LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWIN+KLPPP KDELMVKKLCVVPKSEI
Sbjct: 232 LEYSSREEHYNKIRTCIPKYKGDDISQLTDINWINSKLPPPKKDELMVKKLCVVPKSEI 290
>gb|ORX92582.1| TIP41-domain-containing protein [Basidiobolus meristosporus CBS
931.73]
Length = 286
Score = 136 bits (342), Expect = 4e-36
Identities = 66/99 (66%), Positives = 81/99 (81%)
Frame = -1
Query: 542 IDLFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQL 363
I L+EDELADNG+AIL++KIRVMP+GF+ILQRFFLRVD+VLFR+NDTR+YH F T YL
Sbjct: 171 IVLYEDELADNGSAILSIKIRVMPTGFFILQRFFLRVDDVLFRVNDTRLYHAFDTSYLIK 230
Query: 362 EYSSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLPP 246
EY+ REE Y IR IP +K DDIS L+D NW+ + +PP
Sbjct: 231 EYTFREEPYINIRKKIPVWKKDDISLLSDPNWVVSVMPP 269
>gb|KFH69603.1| hypothetical protein MVEG_04409 [Mortierella verticillata NRRL
6337]
Length = 271
Score = 129 bits (324), Expect = 1e-33
Identities = 64/112 (57%), Positives = 84/112 (75%)
Frame = -1
Query: 536 LFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQLEY 357
LFEDELADNGTA+L K+RVMPS F+IL RFFLRVD+VLFR+ DTR+YH FGT+ + E
Sbjct: 162 LFEDELADNGTAVLTTKVRVMPSCFFILLRFFLRVDDVLFRIYDTRIYHRFGTNTVVREC 221
Query: 356 SSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLPPPNKDELMVKKLCVVP 201
S+RE Y + R+ IP+ KGDD+S L D NW++++LP + E V+ L + P
Sbjct: 222 STRESSYRETRSRIPRLKGDDLSLLLDSNWVSSQLP---EKESQVEVLSLSP 270
>ref|XP_018297796.1| hypothetical protein PHYBLDRAFT_179237 [Phycomyces blakesleeanus
NRRL 1555(-)]
gb|OAD79756.1| hypothetical protein PHYBLDRAFT_179237 [Phycomyces blakesleeanus
NRRL 1555(-)]
Length = 273
Score = 128 bits (322), Expect = 3e-33
Identities = 64/109 (58%), Positives = 84/109 (77%), Gaps = 4/109 (3%)
Frame = -1
Query: 536 LFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQLEY 357
L+EDELADNGTA+L++++RVMP+ F ILQRFF+RVD+VLFR+NDTRVYHEFG+ YL EY
Sbjct: 164 LYEDELADNGTAMLSIRLRVMPTCFLILQRFFMRVDDVLFRINDTRVYHEFGSKYLVREY 223
Query: 356 SSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLPPP----NKDELMV 222
+S+E+HYN IR +P DIS L D NW++ LP +++ LMV
Sbjct: 224 TSKEDHYNNIRRKLP-IGNMDISLLNDPNWVSRMLPDEPAVFSRESLMV 271
>ref|XP_016609180.1| hypothetical protein SPPG_04232, partial [Spizellomyces punctatus
DAOM BR117]
gb|KND01141.1| hypothetical protein SPPG_04232, partial [Spizellomyces punctatus
DAOM BR117]
Length = 304
Score = 127 bits (318), Expect = 2e-32
Identities = 65/106 (61%), Positives = 83/106 (78%), Gaps = 8/106 (7%)
Frame = -1
Query: 545 EIDLFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 366
E+ L+EDELADNGTAIL++++RVMPS F IL RFFLRVD+VLFR+NDTR YH+FGT++L
Sbjct: 160 ELILYEDELADNGTAILSLRVRVMPSCFLILLRFFLRVDDVLFRINDTRFYHQFGTNHLL 219
Query: 365 LEYSSREEHYNKIRTCIPK-------YKG-DDISQLTDINWINTKL 252
EYSSREE Y+K+R +PK KG +D+S LTD NW+ + L
Sbjct: 220 REYSSREEPYSKVRAKLPKPPPWEVHKKGTEDLSPLTDANWVASVL 265
>ref|XP_021881223.1| TIP41-like family-domain-containing protein [Lobosporangium
transversale]
gb|ORZ15475.1| TIP41-like family-domain-containing protein [Lobosporangium
transversale]
Length = 278
Score = 126 bits (316), Expect = 3e-32
Identities = 59/96 (61%), Positives = 76/96 (79%)
Frame = -1
Query: 536 LFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQLEY 357
LFEDELADNGTA+L K+RVMPS F IL RFFLRVD+VLFR++DTR+YH FGT+ + E
Sbjct: 170 LFEDELADNGTAVLTTKVRVMPSCFLILLRFFLRVDDVLFRIHDTRIYHRFGTNAVVREC 229
Query: 356 SSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLP 249
S+RE Y +IR IP+ G+D+S L D NW++++LP
Sbjct: 230 STRESSYKEIRAKIPRSNGEDLSLLLDSNWVSSQLP 265
>gb|EPB87275.1| hypothetical protein HMPREF1544_05896 [Mucor circinelloides f.
circinelloides 1006PhL]
Length = 269
Score = 125 bits (315), Expect = 3e-32
Identities = 62/110 (56%), Positives = 84/110 (76%), Gaps = 4/110 (3%)
Frame = -1
Query: 536 LFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQLEY 357
L+EDELADNGTA+L +++RVMPS F +LQRFFLRVD+VLFR+NDTR+Y+EFG +Y EY
Sbjct: 161 LYEDELADNGTAMLTIRLRVMPSCFLVLQRFFLRVDDVLFRINDTRLYYEFGKEYFVREY 220
Query: 356 SSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLPPP----NKDELMVK 219
+S+E HYN+IR I +G DIS L D NW+++ +P +++L VK
Sbjct: 221 TSKEVHYNEIRQAIKLGRG-DISLLNDQNWVSSVMPEEPIICKREKLFVK 269
>gb|ORZ10766.1| TIP41-like family-domain-containing protein [Absidia repens]
Length = 266
Score = 125 bits (314), Expect = 4e-32
Identities = 60/91 (65%), Positives = 74/91 (81%)
Frame = -1
Query: 536 LFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQLEY 357
L+EDELADNGTA+L+V++RVMPS F +LQRFFLRVD+VLFR+NDTR+YHEFG+ YL EY
Sbjct: 159 LYEDELADNGTAMLSVRLRVMPSCFLVLQRFFLRVDDVLFRINDTRIYHEFGSPYLVREY 218
Query: 356 SSREEHYNKIRTCIPKYKGDDISQLTDINWI 264
+S+E+HY IR K +G DI L D NWI
Sbjct: 219 TSKEDHYMNIR---KKMEGKDIGLLNDANWI 246
>gb|OON07213.1| hypothetical protein, variant [Batrachochytrium salamandrivorans]
Length = 236
Score = 124 bits (311), Expect = 5e-32
Identities = 65/123 (52%), Positives = 83/123 (67%), Gaps = 6/123 (4%)
Frame = -1
Query: 545 EIDLFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 366
E+ LFEDELADNG AILN+++RVM S F +LQRFF+RVD V+FR+NDTR+YHEFGTD L
Sbjct: 113 ELVLFEDELADNGIAILNIRVRVMKSCFLVLQRFFMRVDGVMFRINDTRLYHEFGTDRLI 172
Query: 365 LEYSSREEHYNKIRTCIP------KYKGDDISQLTDINWINTKLPPPNKDELMVKKLCVV 204
EYSSRE Y+ ++ +P K D+SQLTD+ W+ + L K + VK V
Sbjct: 173 REYSSREAPYDMVKQHLPTPKDMSKTSMQDLSQLTDVGWVASILEKNTKPVVFVKDCLKV 232
Query: 203 PKS 195
S
Sbjct: 233 QSS 235
>gb|OON07212.1| hypothetical protein BSLG_03270 [Batrachochytrium salamandrivorans]
Length = 290
Score = 124 bits (311), Expect = 2e-31
Identities = 65/123 (52%), Positives = 83/123 (67%), Gaps = 6/123 (4%)
Frame = -1
Query: 545 EIDLFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQ 366
E+ LFEDELADNG AILN+++RVM S F +LQRFF+RVD V+FR+NDTR+YHEFGTD L
Sbjct: 167 ELVLFEDELADNGIAILNIRVRVMKSCFLVLQRFFMRVDGVMFRINDTRLYHEFGTDRLI 226
Query: 365 LEYSSREEHYNKIRTCIP------KYKGDDISQLTDINWINTKLPPPNKDELMVKKLCVV 204
EYSSRE Y+ ++ +P K D+SQLTD+ W+ + L K + VK V
Sbjct: 227 REYSSREAPYDMVKQHLPTPKDMSKTSMQDLSQLTDVGWVASILEKNTKPVVFVKDCLKV 286
Query: 203 PKS 195
S
Sbjct: 287 QSS 289
>gb|OAQ24169.1| TIP41-domain-containing protein [Mortierella elongata AG-77]
Length = 269
Score = 123 bits (308), Expect = 3e-31
Identities = 60/96 (62%), Positives = 73/96 (76%)
Frame = -1
Query: 536 LFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQLEY 357
LFEDELADNGTA+L+ K+RVMPS +IL RFFLRVDEVLFR+ DTR+YH FGT + E
Sbjct: 162 LFEDELADNGTAVLSTKVRVMPSCLFILLRFFLRVDEVLFRIYDTRIYHRFGTKTVIRES 221
Query: 356 SSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLP 249
S RE Y R IP+ KGDD+S L D NW++++LP
Sbjct: 222 SFRESSYLDTRARIPRSKGDDLSLLLDSNWVSSQLP 257
>emb|CDH52482.1| type 2a phosphatase activator tip41 [Lichtheimia corymbifera
JMRC:FSU:9682]
Length = 267
Score = 122 bits (305), Expect = 9e-31
Identities = 56/96 (58%), Positives = 75/96 (78%)
Frame = -1
Query: 536 LFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQLEY 357
L+EDELADNGT IL+V++R+MPS F +LQR FLRVD+VLFR+NDTR+YH FGT Y+ EY
Sbjct: 159 LYEDELADNGTTILSVRLRIMPSCFLVLQRLFLRVDDVLFRINDTRIYHTFGTPYVIREY 218
Query: 356 SSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLP 249
+S+E+HY I +P KG D+ + D NW+++ LP
Sbjct: 219 TSKEDHYKTIINKLPMTKG-DVPLVNDPNWVSSSLP 253
>emb|CDS05921.1| hypothetical protein LRAMOSA08449 [Lichtheimia ramosa]
Length = 267
Score = 121 bits (304), Expect = 1e-30
Identities = 56/96 (58%), Positives = 76/96 (79%)
Frame = -1
Query: 536 LFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQLEY 357
L+EDELADNGT +L+V++RVMPS F +LQR FLRVD+VLFR+NDTR+YH FGT Y+ EY
Sbjct: 159 LYEDELADNGTTMLSVRLRVMPSCFLVLQRLFLRVDDVLFRINDTRIYHTFGTPYVIREY 218
Query: 356 SSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLP 249
+S+E+HY I + +P KG D+ + D NW+++ LP
Sbjct: 219 TSKEDHYRTIISKLPMAKG-DVPLVNDPNWVSSSLP 253
>emb|SAM05970.1| hypothetical protein [Absidia glauca]
Length = 357
Score = 123 bits (308), Expect = 2e-30
Identities = 60/96 (62%), Positives = 74/96 (77%)
Frame = -1
Query: 536 LFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQLEY 357
L+EDELADNGTA+L+V++RVMPS F ILQRFFLRVD+VLFR+NDTR+YHEFG+ YL EY
Sbjct: 250 LYEDELADNGTAVLSVRMRVMPSCFLILQRFFLRVDDVLFRINDTRLYHEFGSPYLVREY 309
Query: 356 SSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLP 249
S+E+HY IR K D+ L D NWI + +P
Sbjct: 310 ISKEDHYKNIR---KKLGDSDVGLLNDANWIASIIP 342
>emb|CEP12211.1| hypothetical protein [Parasitella parasitica]
Length = 267
Score = 120 bits (301), Expect = 4e-30
Identities = 58/96 (60%), Positives = 74/96 (77%)
Frame = -1
Query: 536 LFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQLEY 357
L+EDELADNGTA+L +++RVMP+ F ILQRFFLRVD+VLFR+NDTR+YHEFG Y EY
Sbjct: 161 LYEDELADNGTAMLTIRLRVMPTCFLILQRFFLRVDDVLFRINDTRLYHEFGNKYFVREY 220
Query: 356 SSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLP 249
+S+E HYN IR K DIS L D +W+++ +P
Sbjct: 221 TSKEAHYNLIR---QKLGRGDISLLNDQDWVSSAMP 253
>gb|EIE84533.1| hypothetical protein RO3G_09243 [Rhizopus delemar RA 99-880]
Length = 286
Score = 120 bits (301), Expect = 5e-30
Identities = 58/100 (58%), Positives = 74/100 (74%)
Frame = -1
Query: 536 LFEDELADNGTAILNVKIRVMPSGFYILQRFFLRVDEVLFRMNDTRVYHEFGTDYLQLEY 357
L+EDELADNGTA+L V++RVMP+ F +LQRFF+RVD VLFR+NDTR+YHEFG Y EY
Sbjct: 181 LYEDELADNGTAMLTVRLRVMPTCFLVLQRFFMRVDNVLFRINDTRLYHEFGNPYFVREY 240
Query: 356 SSREEHYNKIRTCIPKYKGDDISQLTDINWINTKLPPPNK 237
+S+E HYN+I + K DIS L D NW+ + +P K
Sbjct: 241 TSKEAHYNEI---LEKLGKGDISLLNDQNWVTSVMPDEAK 277