BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon26_contig00034776
         (1048 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ONK71408.1| uncharacterized protein A4U43_C04F8240 [Asparagus...   448   e-143
ref|XP_020260503.1| LOW QUALITY PROTEIN: putative E3 ubiquitin-p...   448   e-142
ref|XP_008788369.1| PREDICTED: putative E3 ubiquitin-protein lig...   409   e-127
ref|XP_008788367.1| PREDICTED: putative E3 ubiquitin-protein lig...   409   e-127
ref|XP_010920190.1| PREDICTED: putative E3 ubiquitin-protein lig...   404   e-126
gb|KDP36916.1| hypothetical protein JCGZ_08207 [Jatropha curcas]      368   e-123
gb|PKI41218.1| hypothetical protein CRG98_038391 [Punica granatum]    350   e-115
ref|XP_021612760.1| putative E3 ubiquitin-protein ligase LIN-1 [...   369   e-113
gb|PON93899.1| E3 ubiquitin ligase [Trema orientalis]                 369   e-113
ref|XP_020535434.1| putative E3 ubiquitin-protein ligase LIN-1 i...   368   e-113
ref|XP_020535433.1| putative E3 ubiquitin-protein ligase LIN-1 i...   368   e-113
gb|ERN13438.1| hypothetical protein AMTR_s00041p00198080 [Ambore...   348   e-113
ref|XP_017976783.1| PREDICTED: putative E3 ubiquitin-protein lig...   367   e-112
ref|XP_021692655.1| putative E3 ubiquitin-protein ligase LIN-1 i...   367   e-112
ref|XP_021692654.1| putative E3 ubiquitin-protein ligase LIN-1 i...   367   e-112
gb|EOY07584.1| Transducin/WD40 repeat-like superfamily protein [...   365   e-112
gb|OVA06309.1| WD40 repeat [Macleaya cordata]                         364   e-111
ref|XP_021289658.1| putative E3 ubiquitin-protein ligase LIN-1 [...   363   e-111
emb|CAN74785.1| hypothetical protein VITISV_011516 [Vitis vinifera]   362   e-110
gb|PKA66456.1| Putative E3 ubiquitin-protein ligase LIN-1 [Apost...   362   e-110

>gb|ONK71408.1| uncharacterized protein A4U43_C04F8240 [Asparagus officinalis]
          Length = 1360

 Score =  448 bits (1153), Expect = e-143
 Identities = 218/259 (84%), Positives = 234/259 (90%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAGRRGLR VQE+REHSKAVTCLY+PSSG KLYS S DKTIRVWEI PEEIL VQ+HD
Sbjct: 1102 VWDAGRRGLRSVQEIREHSKAVTCLYVPSSGNKLYSSSLDKTIRVWEITPEEILIVQIHD 1161

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            MKETVQCLT  ADLVC+ASQGTGVKVYNGSG PKHVN  K+VKCLA  E +LYCGCT+YS
Sbjct: 1162 MKETVQCLTATADLVCYASQGTGVKVYNGSGNPKHVNLGKNVKCLAVEEGNLYCGCTSYS 1221

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEV LSKCTS  LYSGTRKLLGKQTI AL IQDG+LYAGGSSVDGTAGKAFSL+TK A+
Sbjct: 1222 IQEVGLSKCTSTMLYSGTRKLLGKQTILALQIQDGVLYAGGSSVDGTAGKAFSLSTKTAI 1281

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSFTTGIDIHC+T SSDFVFTGT+CG+IEVWLRERLTRVGSIKVG+G + KVT LASDSD
Sbjct: 1282 GSFTTGIDIHCMTVSSDFVFTGTKCGIIEVWLRERLTRVGSIKVGNGSNTKVTTLASDSD 1341

Query: 327  GEMLFSGTADGKIQVWGLD 271
            GEMLFSG+ADGKIQVW LD
Sbjct: 1342 GEMLFSGSADGKIQVWALD 1360


>ref|XP_020260503.1| LOW QUALITY PROTEIN: putative E3 ubiquitin-protein ligase LIN-1
            [Asparagus officinalis]
          Length = 1520

 Score =  448 bits (1153), Expect = e-142
 Identities = 218/259 (84%), Positives = 234/259 (90%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAGRRGLR VQE+REHSKAVTCLY+PSSG KLYS S DKTIRVWEI PEEIL VQ+HD
Sbjct: 1262 VWDAGRRGLRSVQEIREHSKAVTCLYVPSSGNKLYSSSLDKTIRVWEITPEEILIVQIHD 1321

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            MKETVQCLT  ADLVC+ASQGTGVKVYNGSG PKHVN  K+VKCLA  E +LYCGCT+YS
Sbjct: 1322 MKETVQCLTATADLVCYASQGTGVKVYNGSGNPKHVNLGKNVKCLAVEEGNLYCGCTSYS 1381

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEV LSKCTS  LYSGTRKLLGKQTI AL IQDG+LYAGGSSVDGTAGKAFSL+TK A+
Sbjct: 1382 IQEVGLSKCTSTMLYSGTRKLLGKQTILALQIQDGVLYAGGSSVDGTAGKAFSLSTKTAI 1441

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSFTTGIDIHC+T SSDFVFTGT+CG+IEVWLRERLTRVGSIKVG+G + KVT LASDSD
Sbjct: 1442 GSFTTGIDIHCMTVSSDFVFTGTKCGIIEVWLRERLTRVGSIKVGNGSNTKVTTLASDSD 1501

Query: 327  GEMLFSGTADGKIQVWGLD 271
            GEMLFSG+ADGKIQVW LD
Sbjct: 1502 GEMLFSGSADGKIQVWALD 1520


>ref|XP_008788369.1| PREDICTED: putative E3 ubiquitin-protein ligase LIN isoform X2
            [Phoenix dactylifera]
          Length = 1520

 Score =  409 bits (1050), Expect = e-127
 Identities = 196/259 (75%), Positives = 223/259 (86%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VW+ GRR L+L+QEV  HSKAVTCLYIP SG KLYSGSHDKTIRVW IGP++I  VQVHD
Sbjct: 1262 VWETGRRVLKLIQEVHAHSKAVTCLYIPPSGDKLYSGSHDKTIRVWAIGPDKIRPVQVHD 1321

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
             K+TV CL   A+L+CF SQGTG KVY+ +G+PKHVNF+K+VKCLA  E+SLYCGC+ YS
Sbjct: 1322 TKDTVNCLAANANLLCFTSQGTGAKVYDWNGSPKHVNFNKNVKCLAIMEDSLYCGCSGYS 1381

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL KC SNT YSGTRKLLGKQTIHAL IQDGIL+AGGSSVDGTAGKAFSL+TK  V
Sbjct: 1382 IQEVDLRKCRSNTFYSGTRKLLGKQTIHALCIQDGILFAGGSSVDGTAGKAFSLSTKTPV 1441

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF+T +DIHCI  S+DFVFTGT+CGVIEVWLR RLTRV SIKVG G + KVT +ASD+D
Sbjct: 1442 GSFSTALDIHCIYVSNDFVFTGTKCGVIEVWLRARLTRVASIKVGGGANTKVTTVASDAD 1501

Query: 327  GEMLFSGTADGKIQVWGLD 271
            GE+LFSG+ADGKIQVW L+
Sbjct: 1502 GELLFSGSADGKIQVWALE 1520


>ref|XP_008788367.1| PREDICTED: putative E3 ubiquitin-protein ligase LIN isoform X1
            [Phoenix dactylifera]
          Length = 1552

 Score =  409 bits (1050), Expect = e-127
 Identities = 196/259 (75%), Positives = 223/259 (86%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VW+ GRR L+L+QEV  HSKAVTCLYIP SG KLYSGSHDKTIRVW IGP++I  VQVHD
Sbjct: 1294 VWETGRRVLKLIQEVHAHSKAVTCLYIPPSGDKLYSGSHDKTIRVWAIGPDKIRPVQVHD 1353

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
             K+TV CL   A+L+CF SQGTG KVY+ +G+PKHVNF+K+VKCLA  E+SLYCGC+ YS
Sbjct: 1354 TKDTVNCLAANANLLCFTSQGTGAKVYDWNGSPKHVNFNKNVKCLAIMEDSLYCGCSGYS 1413

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL KC SNT YSGTRKLLGKQTIHAL IQDGIL+AGGSSVDGTAGKAFSL+TK  V
Sbjct: 1414 IQEVDLRKCRSNTFYSGTRKLLGKQTIHALCIQDGILFAGGSSVDGTAGKAFSLSTKTPV 1473

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF+T +DIHCI  S+DFVFTGT+CGVIEVWLR RLTRV SIKVG G + KVT +ASD+D
Sbjct: 1474 GSFSTALDIHCIYVSNDFVFTGTKCGVIEVWLRARLTRVASIKVGGGANTKVTTVASDAD 1533

Query: 327  GEMLFSGTADGKIQVWGLD 271
            GE+LFSG+ADGKIQVW L+
Sbjct: 1534 GELLFSGSADGKIQVWALE 1552


>ref|XP_010920190.1| PREDICTED: putative E3 ubiquitin-protein ligase LIN-1 [Elaeis
            guineensis]
          Length = 1550

 Score =  404 bits (1039), Expect = e-126
 Identities = 194/259 (74%), Positives = 222/259 (85%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VW+ GRR L+L+QEVR HSKAVTCLYIPSSG KLYSGSHDKTIRVW I P+EI  VQ+HD
Sbjct: 1292 VWETGRRVLKLIQEVRAHSKAVTCLYIPSSGDKLYSGSHDKTIRVWAIRPDEIHPVQIHD 1351

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
             K+TV CL   A+L+CF SQGTG KVY+ +G+PKHVNF+K+VKCLA  E+SLYCGC+ YS
Sbjct: 1352 TKDTVNCLAANANLLCFTSQGTGAKVYDWNGSPKHVNFNKNVKCLAIMEDSLYCGCSGYS 1411

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL KC SNT YSGTRKLLGKQTIHAL IQD IL+AGGSSVDGTAGKAFSL+TK  V
Sbjct: 1412 IQEVDLRKCRSNTFYSGTRKLLGKQTIHALFIQDDILFAGGSSVDGTAGKAFSLSTKTPV 1471

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
             SF+T +DI CI  ++DFVFTGT+CG+IEVWLRERLTRV SIKVG G + KVT LASDSD
Sbjct: 1472 RSFSTALDIQCIIVNNDFVFTGTKCGIIEVWLRERLTRVASIKVGGGANTKVTTLASDSD 1531

Query: 327  GEMLFSGTADGKIQVWGLD 271
            GE+LFSG+ADG+IQVW L+
Sbjct: 1532 GELLFSGSADGRIQVWALE 1550


>gb|KDP36916.1| hypothetical protein JCGZ_08207 [Jatropha curcas]
          Length = 390

 Score =  368 bits (945), Expect = e-123
 Identities = 178/259 (68%), Positives = 208/259 (80%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAG+RGLRL+ EVREH+KAVTCLY+PSSG KLYSGS DKT+RVW I PEEI  VQVHD
Sbjct: 132  VWDAGKRGLRLIHEVREHTKAVTCLYVPSSGDKLYSGSLDKTVRVWAIKPEEIHCVQVHD 191

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  LT  A + CF SQGTGVKVYN SG PKHV F+K VKCLA   + LYCGC+ YS
Sbjct: 192  VKEVVYELTANAKVACFISQGTGVKVYNWSGVPKHVTFNKTVKCLAMTGDKLYCGCSGYS 251

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL K TS T YSGTRKLLGKQ IH+LHI DG+L AGGS++DGTAGK FS +TK  V
Sbjct: 252  IQEVDLCKLTSTTFYSGTRKLLGKQIIHSLHIHDGLLLAGGSAIDGTAGKVFSHSTKAVV 311

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF+TG+DI  I A++DF+FT T+CG IEVWL+ER+TRV SIKV  G  +K+T+L SD D
Sbjct: 312  GSFSTGMDILRIVANNDFIFTATKCGAIEVWLKERVTRVASIKVTGGSHSKMTSLTSDMD 371

Query: 327  GEMLFSGTADGKIQVWGLD 271
            G ML++G +DGKIQ W +D
Sbjct: 372  GGMLYAGYSDGKIQAWAMD 390


>gb|PKI41218.1| hypothetical protein CRG98_038391 [Punica granatum]
          Length = 391

 Score =  350 bits (897), Expect = e-115
 Identities = 166/260 (63%), Positives = 205/260 (78%), Gaps = 1/260 (0%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKL-YSGSHDKTIRVWEIGPEEILSVQVH 871
            +WD  +R LRL+QEVREH+KAVTCL +PS G +L YSGS DKTIRVW + PEEI  VQVH
Sbjct: 132  MWDTSKRSLRLIQEVREHAKAVTCLCLPSLGDRLFYSGSLDKTIRVWALKPEEIHCVQVH 191

Query: 870  DMKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAY 691
            D+KE V  LT  +++ CF SQGTG+KVYN SG+PKHVNF+K  KCLA   + LYCGC+ Y
Sbjct: 192  DVKEPVHGLTANSNVACFISQGTGIKVYNWSGSPKHVNFNKTAKCLAMFGDKLYCGCSGY 251

Query: 690  SIQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKA 511
            ++QEVDL K  S+  YSGTRKLLGKQTIHALH+ D  L+ GGSSVDGTAGK FS  +K  
Sbjct: 252  NVQEVDLGKLVSSPFYSGTRKLLGKQTIHALHVNDNFLFCGGSSVDGTAGKVFSFPSKAV 311

Query: 510  VGSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDS 331
            VG+F+TG +I  I AS+DF+FT T+CG+IEVWLRER++R+ SIK+  GG  K+T+LASD 
Sbjct: 312  VGAFSTGFEIQHIAASNDFIFTATKCGIIEVWLRERISRLASIKMACGGHHKITSLASDI 371

Query: 330  DGEMLFSGTADGKIQVWGLD 271
            DG MLF+G++DG+IQ W LD
Sbjct: 372  DGGMLFAGSSDGRIQAWALD 391


>ref|XP_021612760.1| putative E3 ubiquitin-protein ligase LIN-1 [Manihot esculenta]
 ref|XP_021612761.1| putative E3 ubiquitin-protein ligase LIN-1 [Manihot esculenta]
 ref|XP_021612762.1| putative E3 ubiquitin-protein ligase LIN-1 [Manihot esculenta]
 ref|XP_021612763.1| putative E3 ubiquitin-protein ligase LIN-1 [Manihot esculenta]
 ref|XP_021612764.1| putative E3 ubiquitin-protein ligase LIN-1 [Manihot esculenta]
 ref|XP_021612765.1| putative E3 ubiquitin-protein ligase LIN-1 [Manihot esculenta]
 gb|OAY50573.1| hypothetical protein MANES_05G146700 [Manihot esculenta]
 gb|OAY50574.1| hypothetical protein MANES_05G146700 [Manihot esculenta]
          Length = 1496

 Score =  369 bits (947), Expect = e-113
 Identities = 179/259 (69%), Positives = 207/259 (79%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAG+R LRL+QEVREH+KAVTCLYIPSSG KLYSGS DKTIRVW I PEEI  VQVHD
Sbjct: 1238 VWDAGKRVLRLIQEVREHTKAVTCLYIPSSGDKLYSGSLDKTIRVWAIKPEEIHCVQVHD 1297

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  LT  + + CF SQGTGVKVYN SG PKHV F+K VKCLA   + LYCGC+ YS
Sbjct: 1298 VKEAVYELTANSKVACFMSQGTGVKVYNWSGVPKHVTFNKTVKCLAMTGDKLYCGCSGYS 1357

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL K TS T YSGTRKLLGKQ IH+LHI DG+L+AGGS++DGTAGK FS TTK  V
Sbjct: 1358 IQEVDLCKLTSTTFYSGTRKLLGKQIIHSLHIHDGLLFAGGSAIDGTAGKVFSHTTKAMV 1417

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF TG DI  I A++DF+FT T+CG IEVWL+ER+TRV SIK+   G  K+T+L SD D
Sbjct: 1418 GSFWTGFDIMHIAANNDFIFTATKCGTIEVWLKERITRVASIKMSGSGHPKITSLTSDMD 1477

Query: 327  GEMLFSGTADGKIQVWGLD 271
            G ML++G++DGKIQ W +D
Sbjct: 1478 GGMLYAGSSDGKIQAWAMD 1496


>gb|PON93899.1| E3 ubiquitin ligase [Trema orientalis]
          Length = 1490

 Score =  369 bits (946), Expect = e-113
 Identities = 180/260 (69%), Positives = 211/260 (81%), Gaps = 1/260 (0%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAG+R LRL+QEVREH+KAVTCLY  SSG KLYSGS DKTIRVW + PEEIL VQVHD
Sbjct: 1231 VWDAGKRILRLIQEVREHTKAVTCLYASSSGDKLYSGSLDKTIRVWAVKPEEILCVQVHD 1290

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLA-TGEESLYCGCTAY 691
            +KE V  LT    + CF SQGTGVK+Y+ SGTPKH+NF+K+VKCLA TG   LYCGC+ Y
Sbjct: 1291 VKEAVHDLTANTKVACFISQGTGVKIYDWSGTPKHINFNKYVKCLAMTGNNKLYCGCSGY 1350

Query: 690  SIQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKA 511
            SIQEVDLS  TSNT YSGTRKLLGKQTI++L I DG+L+AGGSSVDGTAGK F L+TK  
Sbjct: 1351 SIQEVDLSTYTSNTFYSGTRKLLGKQTIYSLQIHDGVLFAGGSSVDGTAGKIFLLSTKAI 1410

Query: 510  VGSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDS 331
            VGSF TG DI  I  ++DF+FTG+RCG I+VWL+ER TRV SIK+  GG +K T+L SD+
Sbjct: 1411 VGSFPTGFDIQRIAINNDFIFTGSRCGNIDVWLKERFTRVASIKMSCGGHSKFTSLTSDT 1470

Query: 330  DGEMLFSGTADGKIQVWGLD 271
            DG ML++ ++DGKIQVW LD
Sbjct: 1471 DGGMLYAASSDGKIQVWDLD 1490


>ref|XP_020535434.1| putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Jatropha
            curcas]
          Length = 1489

 Score =  368 bits (945), Expect = e-113
 Identities = 178/259 (68%), Positives = 208/259 (80%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAG+RGLRL+ EVREH+KAVTCLY+PSSG KLYSGS DKT+RVW I PEEI  VQVHD
Sbjct: 1231 VWDAGKRGLRLIHEVREHTKAVTCLYVPSSGDKLYSGSLDKTVRVWAIKPEEIHCVQVHD 1290

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  LT  A + CF SQGTGVKVYN SG PKHV F+K VKCLA   + LYCGC+ YS
Sbjct: 1291 VKEVVYELTANAKVACFISQGTGVKVYNWSGVPKHVTFNKTVKCLAMTGDKLYCGCSGYS 1350

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL K TS T YSGTRKLLGKQ IH+LHI DG+L AGGS++DGTAGK FS +TK  V
Sbjct: 1351 IQEVDLCKLTSTTFYSGTRKLLGKQIIHSLHIHDGLLLAGGSAIDGTAGKVFSHSTKAVV 1410

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF+TG+DI  I A++DF+FT T+CG IEVWL+ER+TRV SIKV  G  +K+T+L SD D
Sbjct: 1411 GSFSTGMDILRIVANNDFIFTATKCGAIEVWLKERVTRVASIKVTGGSHSKMTSLTSDMD 1470

Query: 327  GEMLFSGTADGKIQVWGLD 271
            G ML++G +DGKIQ W +D
Sbjct: 1471 GGMLYAGYSDGKIQAWAMD 1489


>ref|XP_020535433.1| putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Jatropha
            curcas]
          Length = 1497

 Score =  368 bits (945), Expect = e-113
 Identities = 178/259 (68%), Positives = 208/259 (80%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAG+RGLRL+ EVREH+KAVTCLY+PSSG KLYSGS DKT+RVW I PEEI  VQVHD
Sbjct: 1239 VWDAGKRGLRLIHEVREHTKAVTCLYVPSSGDKLYSGSLDKTVRVWAIKPEEIHCVQVHD 1298

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  LT  A + CF SQGTGVKVYN SG PKHV F+K VKCLA   + LYCGC+ YS
Sbjct: 1299 VKEVVYELTANAKVACFISQGTGVKVYNWSGVPKHVTFNKTVKCLAMTGDKLYCGCSGYS 1358

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL K TS T YSGTRKLLGKQ IH+LHI DG+L AGGS++DGTAGK FS +TK  V
Sbjct: 1359 IQEVDLCKLTSTTFYSGTRKLLGKQIIHSLHIHDGLLLAGGSAIDGTAGKVFSHSTKAVV 1418

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF+TG+DI  I A++DF+FT T+CG IEVWL+ER+TRV SIKV  G  +K+T+L SD D
Sbjct: 1419 GSFSTGMDILRIVANNDFIFTATKCGAIEVWLKERVTRVASIKVTGGSHSKMTSLTSDMD 1478

Query: 327  GEMLFSGTADGKIQVWGLD 271
            G ML++G +DGKIQ W +D
Sbjct: 1479 GGMLYAGYSDGKIQAWAMD 1497


>gb|ERN13438.1| hypothetical protein AMTR_s00041p00198080 [Amborella trichopoda]
          Length = 569

 Score =  348 bits (894), Expect = e-113
 Identities = 168/255 (65%), Positives = 203/255 (79%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWD GRR LRL+QE REH+K VT L +  SG+KLYSGS DKTIRVW IGPEEI  +QV++
Sbjct: 292  VWDTGRRTLRLIQEAREHTKPVTSLSLALSGEKLYSGSLDKTIRVWGIGPEEIHCIQVYE 351

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  LT+  ++ CF SQGTG KVYN SG PK++N +KHVK +A  ++ LYCGCT YS
Sbjct: 352  VKEAVCSLTVNGEMACFISQGTGTKVYNWSGFPKNINSNKHVKTIAMTDDKLYCGCTGYS 411

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL + TS T +SGTRKLLGKQTIH LHI DG+L+AGGSSVDG AGK FSL+TK AV
Sbjct: 412  IQEVDLCRGTSGTFFSGTRKLLGKQTIHVLHINDGLLFAGGSSVDGVAGKVFSLSTKSAV 471

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GS  TG DI+ I  + + +FTGT+CGVIE+WLRER  RVGS+KVG  G+AKVT LA+D D
Sbjct: 472  GSLMTGFDIYSIAVNDELIFTGTKCGVIEIWLRERFLRVGSLKVGVNGNAKVTCLAADPD 531

Query: 327  GEMLFSGTADGKIQV 283
            G+MLF+ ++DGKIQV
Sbjct: 532  GDMLFATSSDGKIQV 546


>ref|XP_017976783.1| PREDICTED: putative E3 ubiquitin-protein ligase LIN-1 [Theobroma
            cacao]
          Length = 1480

 Score =  367 bits (943), Expect = e-112
 Identities = 172/259 (66%), Positives = 209/259 (80%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWD+G+RGLRLVQE REH KAVTCLY+PSSG +LYSGS DKTIR+W + PEEI  +QVHD
Sbjct: 1222 VWDSGKRGLRLVQEAREHMKAVTCLYVPSSGDRLYSGSLDKTIRIWALKPEEIHCIQVHD 1281

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  L+  +   CF SQG GVKVYN +GTPKH+ F+KHVKCLA   + +YCGC+ YS
Sbjct: 1282 VKEAVHDLSANSKFACFISQGNGVKVYNWTGTPKHITFNKHVKCLAITGDKIYCGCSGYS 1341

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQE+DL   T +T YSGTRKLLGKQTI+ALHI DG+LYAGGS+VDG AGK FS ++K  +
Sbjct: 1342 IQELDLCSSTWSTFYSGTRKLLGKQTINALHIDDGLLYAGGSAVDGIAGKVFSRSSKAVM 1401

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF TG DI  I  ++DF+FT T+CG+IEVWL+ER+TRV SIK+GS G AK+T+LASD D
Sbjct: 1402 GSFPTGFDIQQIAVNNDFIFTATKCGIIEVWLKERVTRVASIKMGSKGHAKITSLASDKD 1461

Query: 327  GEMLFSGTADGKIQVWGLD 271
            G MLF+G++DGKIQ W LD
Sbjct: 1462 GGMLFAGSSDGKIQAWSLD 1480


>ref|XP_021692655.1| putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Hevea
            brasiliensis]
          Length = 1497

 Score =  367 bits (942), Expect = e-112
 Identities = 176/259 (67%), Positives = 207/259 (79%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAG+R LRL+QEVREH+KAVTCLY+PSSG KLYSGS DKTIRVW I PEEI  VQ+HD
Sbjct: 1239 VWDAGKRVLRLIQEVREHTKAVTCLYVPSSGDKLYSGSLDKTIRVWAIKPEEIHCVQIHD 1298

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  LT  A + CF SQGTGVKVYN SG PKHV F+K VKCLA   + LYCGC+ YS
Sbjct: 1299 VKEAVCVLTANAKVACFISQGTGVKVYNWSGVPKHVTFNKTVKCLAMTGDKLYCGCSGYS 1358

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL K TS T YSGTRKLLGKQ IH+LHI DG+L+AGGS++DGTAGK FS  TK  V
Sbjct: 1359 IQEVDLCKLTSTTFYSGTRKLLGKQIIHSLHIHDGLLFAGGSAIDGTAGKVFSHCTKAVV 1418

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF TG DI  I  ++DF+FT T+CGVIEVWL+ER+TRV SIK+  GG +K+T+L +D D
Sbjct: 1419 GSFLTGFDILHIVVNNDFIFTATKCGVIEVWLKERITRVASIKMSGGGHSKITSLTADMD 1478

Query: 327  GEMLFSGTADGKIQVWGLD 271
            G ML++ ++DGKIQ W +D
Sbjct: 1479 GGMLYASSSDGKIQAWAVD 1497


>ref|XP_021692654.1| putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Hevea
            brasiliensis]
          Length = 1525

 Score =  367 bits (942), Expect = e-112
 Identities = 176/259 (67%), Positives = 207/259 (79%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAG+R LRL+QEVREH+KAVTCLY+PSSG KLYSGS DKTIRVW I PEEI  VQ+HD
Sbjct: 1267 VWDAGKRVLRLIQEVREHTKAVTCLYVPSSGDKLYSGSLDKTIRVWAIKPEEIHCVQIHD 1326

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  LT  A + CF SQGTGVKVYN SG PKHV F+K VKCLA   + LYCGC+ YS
Sbjct: 1327 VKEAVCVLTANAKVACFISQGTGVKVYNWSGVPKHVTFNKTVKCLAMTGDKLYCGCSGYS 1386

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL K TS T YSGTRKLLGKQ IH+LHI DG+L+AGGS++DGTAGK FS  TK  V
Sbjct: 1387 IQEVDLCKLTSTTFYSGTRKLLGKQIIHSLHIHDGLLFAGGSAIDGTAGKVFSHCTKAVV 1446

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF TG DI  I  ++DF+FT T+CGVIEVWL+ER+TRV SIK+  GG +K+T+L +D D
Sbjct: 1447 GSFLTGFDILHIVVNNDFIFTATKCGVIEVWLKERITRVASIKMSGGGHSKITSLTADMD 1506

Query: 327  GEMLFSGTADGKIQVWGLD 271
            G ML++ ++DGKIQ W +D
Sbjct: 1507 GGMLYASSSDGKIQAWAVD 1525


>gb|EOY07584.1| Transducin/WD40 repeat-like superfamily protein [Theobroma cacao]
          Length = 1500

 Score =  365 bits (937), Expect = e-112
 Identities = 171/259 (66%), Positives = 208/259 (80%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWD+G+RGLRLVQE REH KAVTCLY+PSSG +LYSGS DKTIR+W + PEEI  +QVHD
Sbjct: 1242 VWDSGKRGLRLVQEAREHMKAVTCLYVPSSGDRLYSGSLDKTIRIWALKPEEIHCIQVHD 1301

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  L+  +   CF SQG GVKVYN +GTPKH+ F+KHVKCLA   + +YCGC+ YS
Sbjct: 1302 VKEAVHDLSANSKFACFISQGNGVKVYNWTGTPKHITFNKHVKCLAITGDKIYCGCSGYS 1361

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQE+DL   T +T YSGTRKLLGKQTI+ALHI DG+LYAGGS+VDG AGK FS ++K  +
Sbjct: 1362 IQELDLCSSTWSTFYSGTRKLLGKQTINALHIDDGLLYAGGSAVDGIAGKVFSRSSKAVM 1421

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF T  DI  I  ++DF+FT T+CG+IEVWL+ER+TRV SIK+GS G AK+T+LASD D
Sbjct: 1422 GSFPTAFDIQQIAVNNDFIFTATKCGIIEVWLKERVTRVASIKMGSKGHAKITSLASDKD 1481

Query: 327  GEMLFSGTADGKIQVWGLD 271
            G MLF+G++DGKIQ W LD
Sbjct: 1482 GGMLFAGSSDGKIQAWSLD 1500


>gb|OVA06309.1| WD40 repeat [Macleaya cordata]
          Length = 1480

 Score =  364 bits (935), Expect = e-111
 Identities = 175/260 (67%), Positives = 211/260 (81%), Gaps = 1/260 (0%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAG+R LRL++EVREH+KAVTCL+IPSSG KLYSGS DKTIRVW I  EEI  ++VHD
Sbjct: 1221 VWDAGKRALRLIREVREHTKAVTCLFIPSSGDKLYSGSLDKTIRVWTIKSEEIHCIEVHD 1280

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  L       CFASQGTG+KVYN +G PKH+NF+K+VKCLA  +  LYCGCT YS
Sbjct: 1281 VKEAVHELAANTSFACFASQGTGIKVYNWNGVPKHINFNKNVKCLAMTDNKLYCGCTNYS 1340

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDLSKCTS T +SGT+KLLGKQ I+AL+I DG+L+AGGSSVDG AGK FSL+TK+ +
Sbjct: 1341 IQEVDLSKCTSTTFFSGTKKLLGKQAIYALYIHDGLLFAGGSSVDGIAGKVFSLSTKEII 1400

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGG-SAKVTALASDS 331
            GS  TG DIH I  +++FVFTGT+CG+I++ LRERL RV SIK+G G  ++KVT L SDS
Sbjct: 1401 GSLPTGFDIHSIAVNNEFVFTGTKCGIIDICLRERLIRVASIKLGGGVLNSKVTCLISDS 1460

Query: 330  DGEMLFSGTADGKIQVWGLD 271
            DGEMLF+G++ GKIQ W LD
Sbjct: 1461 DGEMLFAGSSHGKIQAWALD 1480


>ref|XP_021289658.1| putative E3 ubiquitin-protein ligase LIN-1 [Herrania umbratica]
          Length = 1500

 Score =  363 bits (933), Expect = e-111
 Identities = 170/259 (65%), Positives = 207/259 (79%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAG+RGLRLVQE REH KAVTCLY+PSSG +LYSGS DKTIR+W + PEEI  +QVHD
Sbjct: 1242 VWDAGKRGLRLVQEAREHMKAVTCLYVPSSGDRLYSGSLDKTIRIWALKPEEIHCIQVHD 1301

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  L+  +   CF SQG GVKVYN SGTPKH+ F+KHVKCLA     +YCGC+ YS
Sbjct: 1302 VKEAVHDLSANSKFACFISQGNGVKVYNWSGTPKHITFNKHVKCLAITGNKIYCGCSGYS 1361

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQE+DL   T +T YSGTRKLLGKQTI++LH+ DG+LYAGGS+VDG AGK FS ++K  +
Sbjct: 1362 IQELDLGSSTWSTFYSGTRKLLGKQTINSLHVDDGLLYAGGSAVDGIAGKVFSQSSKAVM 1421

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF  G DI  I  ++DF+FT T+CG+IEVWL+ER+TRV SIK+GS G AK+T+LASD D
Sbjct: 1422 GSFPMGFDIQQIAVNNDFIFTATKCGIIEVWLKERVTRVASIKMGSKGHAKITSLASDLD 1481

Query: 327  GEMLFSGTADGKIQVWGLD 271
            G ML++G++DGKIQ W LD
Sbjct: 1482 GGMLYAGSSDGKIQAWSLD 1500


>emb|CAN74785.1| hypothetical protein VITISV_011516 [Vitis vinifera]
          Length = 1494

 Score =  362 bits (928), Expect = e-110
 Identities = 174/259 (67%), Positives = 204/259 (78%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWDAG+R LRL+QEVREH+KAVTCLY  SS  KLYSGS DKTIRVW + PEEI  VQVHD
Sbjct: 1236 VWDAGKRDLRLIQEVREHTKAVTCLYASSSSDKLYSGSLDKTIRVWTVKPEEIHCVQVHD 1295

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            +KE V  LT  A+  CF+SQGTGV VY+ SG PKH+NF+K+VKCL   E+ LYCGCT YS
Sbjct: 1296 VKEAVYQLTANANFACFSSQGTGVNVYSWSGVPKHINFNKYVKCLDMAEDRLYCGCTGYS 1355

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL K TSNT YSG RKLLGKQTI++L I DG LYAGGSSVDGTAGK FSL+TK   
Sbjct: 1356 IQEVDLCKSTSNTFYSGARKLLGKQTIYSLRIHDGFLYAGGSSVDGTAGKVFSLSTKALT 1415

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF TG+DI  +  +SDF+FT  + G+IEVW +E +T+V SIK+G  G AK+ +LASD+D
Sbjct: 1416 GSFLTGLDIQRLAVNSDFIFTAGKSGIIEVWFKETVTKVASIKIGGHGHAKIASLASDTD 1475

Query: 327  GEMLFSGTADGKIQVWGLD 271
            GEMLF+G  DGKI+ W LD
Sbjct: 1476 GEMLFAGFLDGKIRAWALD 1494


>gb|PKA66456.1| Putative E3 ubiquitin-protein ligase LIN-1 [Apostasia shenzhenica]
          Length = 1523

 Score =  362 bits (928), Expect = e-110
 Identities = 182/259 (70%), Positives = 211/259 (81%)
 Frame = -2

Query: 1047 VWDAGRRGLRLVQEVREHSKAVTCLYIPSSGKKLYSGSHDKTIRVWEIGPEEILSVQVHD 868
            VWD G+RG +LVQEV+EH KAVT LYI  S  +LYS S DKTIR W   PE I S+QV+D
Sbjct: 1266 VWDIGKRGWQLVQEVQEHLKAVTGLYISKSSDRLYSCSLDKTIRAWTTEPE-IHSLQVYD 1324

Query: 867  MKETVQCLTLCADLVCFASQGTGVKVYNGSGTPKHVNFSKHVKCLATGEESLYCGCTAYS 688
            MKE V CLT  AD+VCF+SQGTG KV   SG PK VNF+K+VKCLA  + +LYCGCT YS
Sbjct: 1325 MKEPVYCLTANADIVCFSSQGTGAKVSTWSGVPKQVNFNKNVKCLAMTDANLYCGCTGYS 1384

Query: 687  IQEVDLSKCTSNTLYSGTRKLLGKQTIHALHIQDGILYAGGSSVDGTAGKAFSLTTKKAV 508
            IQEVDL K TSNTLYSGTRKLLGKQ IHAL IQD IL+AG SSVDG AGKAFSL+TK A 
Sbjct: 1385 IQEVDLQKYTSNTLYSGTRKLLGKQNIHALCIQDDILFAGWSSVDGIAGKAFSLSTKIAT 1444

Query: 507  GSFTTGIDIHCITASSDFVFTGTRCGVIEVWLRERLTRVGSIKVGSGGSAKVTALASDSD 328
            GSF T +DI+CIT S DFVFTGT+ G+IE+W+++RL RVGSIKVGS G+AKV++LASDS+
Sbjct: 1445 GSFVTALDIYCITVSDDFVFTGTKSGIIEIWIKDRLARVGSIKVGSAGNAKVSSLASDSE 1504

Query: 327  GEMLFSGTADGKIQVWGLD 271
            GEMLFS ++DGKIQVW L+
Sbjct: 1505 GEMLFSASSDGKIQVWALE 1523