BLASTX nr result
ID: Ophiopogon26_contig00031281
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon26_contig00031281
(665 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_020273972.1| isoamylase 2, chloroplastic [Asparagus offic... 181 7e-49
ref|XP_010278620.1| PREDICTED: isoamylase 2, chloroplastic [Nelu... 160 2e-41
ref|XP_020700621.1| isoamylase 2, chloroplastic [Dendrobium cate... 157 2e-40
ref|XP_008799133.1| PREDICTED: isoamylase 2, chloroplastic [Phoe... 154 2e-39
ref|XP_010938750.1| PREDICTED: isoamylase 2, chloroplastic [Elae... 140 1e-34
gb|PIA39269.1| hypothetical protein AQUCO_02600010v1 [Aquilegia ... 140 2e-34
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti... 134 2e-32
gb|PKA66852.1| Isoamylase 2, chloroplastic [Apostasia shenzhenica] 133 5e-32
gb|AOQ26242.1| ISA2 [Actinidia deliciosa] 132 8e-32
ref|XP_009356744.1| PREDICTED: isoamylase 2, chloroplastic-like ... 132 8e-32
ref|XP_020087102.1| isoamylase 2, chloroplastic [Ananas comosus]... 131 2e-31
gb|OAY83378.1| Isoamylase 2, chloroplastic [Ananas comosus] 131 2e-31
ref|XP_008234909.1| PREDICTED: isoamylase 2, chloroplastic [Prun... 130 5e-31
ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium] 129 1e-30
ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber] ... 129 1e-30
ref|XP_020592870.1| isoamylase 2, chloroplastic [Phalaenopsis eq... 128 3e-30
gb|EXB39337.1| Isoamylase 2 [Morus notabilis] 127 5e-30
ref|XP_010090367.2| isoamylase 2, chloroplastic isoform X2 [Moru... 127 5e-30
ref|XP_024017355.1| isoamylase 2, chloroplastic isoform X1 [Moru... 127 5e-30
ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]... 127 5e-30
>ref|XP_020273972.1| isoamylase 2, chloroplastic [Asparagus officinalis]
ref|XP_020273973.1| isoamylase 2, chloroplastic [Asparagus officinalis]
gb|ONK62840.1| uncharacterized protein A4U43_C07F8670 [Asparagus officinalis]
Length = 855
Score = 181 bits (458), Expect = 7e-49
Identities = 104/210 (49%), Positives = 122/210 (58%)
Frame = +1
Query: 34 KQVHPLSNCRPGRKFLEDTTIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFS 213
KQ PL C+ GR AHK +N K+ HNS EQI PG LNYNAE QR S
Sbjct: 31 KQTVPLKICKSGRLNY------AHKPQNAKVFLKPHNSIEQIHPGSDLNYNAETGQRATS 84
Query: 214 YLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQ 393
YLFRTE+GGLVKVLVG NNMKY V I+VSSLP N+ LILSWG+ RSDS+ L AP
Sbjct: 85 YLFRTEIGGLVKVLVGFNNMKYAVRIDVSSLPTRTND--LILSWGVHRSDSNQLDAP--- 139
Query: 394 TSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXXATDS 573
+TP QN G ++TLEF S Q+P +S
Sbjct: 140 ---------------GCTKTPLVQNLKGRFSVTLEFSSTQIPFYLSFLLHSSSDHPRCES 184
Query: 574 GIKTHRKTNFCVPVGISSGRPMPLGFSKSE 663
GI+THR TNFC+PVG+S+G PMPLG S+ E
Sbjct: 185 GIRTHRMTNFCLPVGMSAGHPMPLGVSRCE 214
>ref|XP_010278620.1| PREDICTED: isoamylase 2, chloroplastic [Nelumbo nucifera]
ref|XP_010278628.1| PREDICTED: isoamylase 2, chloroplastic [Nelumbo nucifera]
Length = 890
Score = 160 bits (404), Expect = 2e-41
Identities = 90/195 (46%), Positives = 113/195 (57%)
Frame = +1
Query: 79 LEDTTIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLV 258
L D T +A N+KI +S +S +Q +P LG E++++ +YLFRTE+GG VK+ V
Sbjct: 54 LRDITKNAQWYHNLKIFASSPSSVDQTRPSLGTFTKTEEVEKPLTYLFRTEIGGQVKITV 113
Query: 259 GANNMKYVVNIEVSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGR 438
G N Y V IEVSSLP E L L+WG+FRSDSS L P SQ S +T ST
Sbjct: 114 GRKNTNYTVCIEVSSLPQCNTEDKLFLNWGIFRSDSSCLTLPGSQVSAPETHSST----- 168
Query: 439 ASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVG 618
+ TP Q S G H + LEF+S Q P +S I++HRKTNFCVPVG
Sbjct: 169 --METPLMQKSSGRHFLQLEFESNQAPFYLSFMLLFSSNTATNNSEIRSHRKTNFCVPVG 226
Query: 619 ISSGRPMPLGFSKSE 663
ISSG P PLG S S+
Sbjct: 227 ISSGHPAPLGISFSD 241
>ref|XP_020700621.1| isoamylase 2, chloroplastic [Dendrobium catenatum]
gb|PKU77045.1| Isoamylase 2, chloroplastic [Dendrobium catenatum]
Length = 894
Score = 157 bits (396), Expect = 2e-40
Identities = 94/215 (43%), Positives = 122/215 (56%), Gaps = 1/215 (0%)
Frame = +1
Query: 19 NIVSAKQVHPLSNCRPGRKFLEDTTIDAHKLRNVKISEASHNST-EQIQPGLGLNYNAEK 195
NI + V+ CR RKFL D + D+ + R ++ ST E+ QP L + + EK
Sbjct: 37 NIPLTRPVYAERRCRSCRKFLCDASNDSLQNRTSEMFTGVARSTKEEAQPVLEMTSDFEK 96
Query: 196 LQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHNLILSWGMFRSDSSHL 375
+ +S LFRTE GLVKV VGA MKY V IEV SLP L HNLIL WGMFRSDS
Sbjct: 97 QNKGYSLLFRTEFDGLVKVFVGAIKMKYTVCIEVQSLPQSLTSHNLILFWGMFRSDSLPF 156
Query: 376 YAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXX 555
+ PDS +S+L T++ + + ++TPF ++S H + L+FD VP
Sbjct: 157 HGPDSHSSVLPTTKFKPEASQHLMKTPFVRDSC-RHILVLDFDQNDVP--FYLSFFLQSD 213
Query: 556 XXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKS 660
+ I+THRKTNFCVPVG+ G PM LG S S
Sbjct: 214 GTGQERTIRTHRKTNFCVPVGLGPGYPMALGVSSS 248
>ref|XP_008799133.1| PREDICTED: isoamylase 2, chloroplastic [Phoenix dactylifera]
Length = 853
Score = 154 bits (388), Expect = 2e-39
Identities = 83/176 (47%), Positives = 110/176 (62%)
Frame = +1
Query: 136 SHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLY 315
S +S EQ+ P LG++ + ++LQ+ FSY+FRTE+GGLVKVLVG +M+Y V +EVSSLP
Sbjct: 57 SQSSVEQLAPDLGVSDSTQELQKPFSYMFRTEIGGLVKVLVGTKSMRYAVRVEVSSLPRA 116
Query: 316 LNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITL 495
+ E L+++WGMFRSDSS L A DSQ + F +NSLG + + L
Sbjct: 117 VGEDGLVMNWGMFRSDSSQLLAQDSQVA-------------------FVRNSLGGYMVEL 157
Query: 496 EFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSE 663
EFDSA++P + S I+THRKT FCVPVG+ G PMPLG S S+
Sbjct: 158 EFDSARIP----FYLSFLLSSPPSVSEIRTHRKTKFCVPVGLGPGWPMPLGVSISD 209
>ref|XP_010938750.1| PREDICTED: isoamylase 2, chloroplastic [Elaeis guineensis]
Length = 851
Score = 140 bits (353), Expect = 1e-34
Identities = 77/180 (42%), Positives = 104/180 (57%)
Frame = +1
Query: 124 ISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSS 303
+S++S EQ+ P L ++ + ++L++ FSY+FR E GGL KVLVG +MKY V +EVS
Sbjct: 52 MSQSSVEHVEQLAPDLEVSDSTQELEKPFSYIFRAETGGLAKVLVGVKDMKYAVRVEVSF 111
Query: 304 LPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTH 483
LP + E L+++WGMFRSDS L DSQ + F QNSLG +
Sbjct: 112 LPRAVGEDGLVMNWGMFRSDSKQLLVQDSQVA-------------------FVQNSLGGY 152
Query: 484 AITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSE 663
+ LEFDS ++P + S I+TH+KT FCVPVGI G PMPLG S S+
Sbjct: 153 MVELEFDSVEIP-----FYLSFLLSSPSVSEIRTHKKTKFCVPVGIGPGCPMPLGVSISD 207
>gb|PIA39269.1| hypothetical protein AQUCO_02600010v1 [Aquilegia coerulea]
Length = 886
Score = 140 bits (352), Expect = 2e-34
Identities = 80/180 (44%), Positives = 103/180 (57%)
Frame = +1
Query: 121 KISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVS 300
K+S S S+ I L + + E L+ YLFRT+ GG VKV VG +MKY+V IEVS
Sbjct: 68 KVSALSQGSSYPIGTQLYKSADLEDLENASMYLFRTDAGGQVKVYVGRKHMKYIVYIEVS 127
Query: 301 SLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGT 480
LP +++ L+LSWG++RSDSS + + LD STS + +TPF QNSLG
Sbjct: 128 FLPQCVSKKELLLSWGVYRSDSSAM-------NPLDPQSSTSGAKSSISQTPFPQNSLGA 180
Query: 481 HAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKS 660
+ +EFDS Q P + IK+HRKTNFCVPVG+ SG P PLG S S
Sbjct: 181 RTLQMEFDSIQAPFYFSFLLLCPSSAASDLKEIKSHRKTNFCVPVGLGSGSPAPLGISFS 240
>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
Length = 882
Score = 134 bits (336), Expect = 2e-32
Identities = 74/193 (38%), Positives = 111/193 (57%)
Frame = +1
Query: 82 EDTTIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVG 261
++ T + + N K+S SH + E+ + +E+L+ +YLF TE+GG VKV+VG
Sbjct: 57 QNATRNCFRNINWKVSATSHIAIEETENRYSTT-ESEELESRLNYLFLTEIGGQVKVIVG 115
Query: 262 ANNMKYVVNIEVSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRA 441
N KY+V+IEVSSL LY +++ LILSWG+FRS+SS D Q + + +T+++
Sbjct: 116 KKNKKYIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEI--- 172
Query: 442 SIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGI 621
PF + S G+ A+ L+F++ P + S I++HRKTNFC+PVG
Sbjct: 173 ----PFMERSSGSFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGF 228
Query: 622 SSGRPMPLGFSKS 660
G P PLG S S
Sbjct: 229 GRGYPAPLGLSFS 241
>gb|PKA66852.1| Isoamylase 2, chloroplastic [Apostasia shenzhenica]
Length = 881
Score = 133 bits (334), Expect = 5e-32
Identities = 82/214 (38%), Positives = 113/214 (52%)
Frame = +1
Query: 13 RKNIVSAKQVHPLSNCRPGRKFLEDTTIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAE 192
+ NI+ +QV+ R G F + + +A + ++ A+ +S + +QP + +N E
Sbjct: 35 KSNILLMQQVNAHRRHRTGESFPQGSLKNAVRKPTSRVLAAAPSSQKDVQPVSDVTFNFE 94
Query: 193 KLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHNLILSWGMFRSDSSH 372
Q+ +SYLFRTE G+VKVLV N+MKY V IE+ SLP + +H+LIL W FRSDS +
Sbjct: 95 DQQKEYSYLFRTEFDGMVKVLVRINDMKYTVYIELQSLPKPIIDHDLILCWHTFRSDSEN 154
Query: 373 LYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXX 552
SQ S L ++TP T+ S G H LEF Q+P
Sbjct: 155 SSGLGSQISALSADH--------FMKTPLTRGSSGMHTAVLEFSFQQIP---FYLSFLLQ 203
Query: 553 XXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 654
A S IKT RKT F VPVG+ SG +PLG S
Sbjct: 204 VDGAAKSEIKTERKTYFRVPVGLGSGHSLPLGIS 237
>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
Length = 866
Score = 132 bits (332), Expect = 8e-32
Identities = 79/190 (41%), Positives = 105/190 (55%)
Frame = +1
Query: 91 TIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANN 270
T D+ + RN+K+ S S Q K ++ +YLFRTE+GG VKVLV N
Sbjct: 59 TKDSFQHRNLKVLATSRISVGQ------------KEEKSSAYLFRTEIGGHVKVLVRQKN 106
Query: 271 MKYVVNIEVSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIR 450
+KY V +EVSSL L E L+++WG+FRSDS SQ LD ST D +++
Sbjct: 107 LKYSVYVEVSSLKLKSGEDKLVMNWGIFRSDS-------SQFIPLDLQSSTPDDRSSTVE 159
Query: 451 TPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSG 630
TPF QNSLG I L F+ + P + S +++HRKT+FCVPVG++SG
Sbjct: 160 TPFVQNSLGKLVIELNFEGSLTPFYLSFLLKFPSDGDSKSSDLRSHRKTSFCVPVGLASG 219
Query: 631 RPMPLGFSKS 660
P PLG S S
Sbjct: 220 YPAPLGLSFS 229
>ref|XP_009356744.1| PREDICTED: isoamylase 2, chloroplastic-like [Pyrus x
bretschneideri]
Length = 870
Score = 132 bits (332), Expect = 8e-32
Identities = 74/183 (40%), Positives = 105/183 (57%)
Frame = +1
Query: 112 RNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNI 291
R++K+ S S E ++ + + E+ +V +Y FRTE G LVKV V N KY+VNI
Sbjct: 68 RDLKVYATSRVSVEPMEQKVYTSTETEEAGKVSTYRFRTETGDLVKVFVRMKNAKYIVNI 127
Query: 292 EVSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNS 471
EVSSL L N+ L+LSWG++RSD+S + P + S ++T ++ TPFT+ S
Sbjct: 128 EVSSLHLSSNDRLLVLSWGIYRSDASS-FMPSNFRSSTPADRTT------TLETPFTETS 180
Query: 472 LGTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGF 651
G + LEF++ Q+P ++D I++HRKTNFC PVG S G P PLG
Sbjct: 181 SGRFTLELEFEAKQIPFYLSFILKSPADADSSDLEIRSHRKTNFCFPVGFSRGNPAPLGL 240
Query: 652 SKS 660
S S
Sbjct: 241 SFS 243
>ref|XP_020087102.1| isoamylase 2, chloroplastic [Ananas comosus]
ref|XP_020087103.1| isoamylase 2, chloroplastic [Ananas comosus]
Length = 863
Score = 131 bits (329), Expect = 2e-31
Identities = 76/180 (42%), Positives = 104/180 (57%), Gaps = 1/180 (0%)
Frame = +1
Query: 127 SEASHNSTEQIQPGLGLNYNA-EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSS 303
S AS + ++ P +G+ + E+LQ+ FSY FRT+ G+VKV+V + KYVV EV+
Sbjct: 55 SSASRSPIRRLHPSVGVKIGSKEELQKGFSYTFRTDSNGIVKVVVRSEYGKYVVRFEVTF 114
Query: 304 LPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTH 483
LP + E L+L+W MF SDSS L P SQ S G + + PF QNS G+
Sbjct: 115 LPQFGTEEGLVLNWTMFTSDSSLLLVPCSQAS----------EGTVTAKVPFVQNSSGSF 164
Query: 484 AITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSE 663
L FD+++VP + S I++HRKTNFCVPVG+ SG+PMPLG S S+
Sbjct: 165 LAELVFDASKVP------FYLSFLLNSAGSEIRSHRKTNFCVPVGLGSGQPMPLGVSVSD 218
>gb|OAY83378.1| Isoamylase 2, chloroplastic [Ananas comosus]
Length = 1205
Score = 131 bits (329), Expect = 2e-31
Identities = 76/180 (42%), Positives = 104/180 (57%), Gaps = 1/180 (0%)
Frame = +1
Query: 127 SEASHNSTEQIQPGLGLNYNA-EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSS 303
S AS + ++ P +G+ + E+LQ+ FSY FRT+ G+VKV+V + KYVV EV+
Sbjct: 55 SSASRSPIRRLHPSVGVKIGSKEELQKGFSYTFRTDSNGIVKVVVRSEYGKYVVRFEVTF 114
Query: 304 LPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTH 483
LP + E L+L+W MF SDSS L P SQ S G + + PF QNS G+
Sbjct: 115 LPQFGTEEGLVLNWTMFTSDSSLLLVPCSQAS----------EGTVTAKVPFVQNSSGSF 164
Query: 484 AITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSE 663
L FD+++VP + S I++HRKTNFCVPVG+ SG+PMPLG S S+
Sbjct: 165 LAELVFDASKVP------FYLSFLLNSAGSEIRSHRKTNFCVPVGLGSGQPMPLGVSVSD 218
>ref|XP_008234909.1| PREDICTED: isoamylase 2, chloroplastic [Prunus mume]
Length = 883
Score = 130 bits (326), Expect = 5e-31
Identities = 72/182 (39%), Positives = 100/182 (54%)
Frame = +1
Query: 115 NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 294
N+K+ S S E ++ E++ +V +YLFRTE+G +V V V N KY+VNIE
Sbjct: 68 NLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFVRKRNAKYIVNIE 127
Query: 295 VSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 474
V SL L ++ L+L WGM+R+DSS + P LD ST + ++ TPF Q S
Sbjct: 128 VPSLYLSSSDRRLVLHWGMYRADSS-CFMP------LDFKSSTPNDTTTTLETPFIQTSS 180
Query: 475 GTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 654
G + LEF++ Q+P +D I++HRKTNFCVPVG G P PLG +
Sbjct: 181 GRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLT 240
Query: 655 KS 660
S
Sbjct: 241 FS 242
>ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium]
Length = 883
Score = 129 bits (324), Expect = 1e-30
Identities = 72/182 (39%), Positives = 99/182 (54%)
Frame = +1
Query: 115 NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 294
N+K+ S S E ++ E++ +V +YLFRTE+G +V V V N KY VNIE
Sbjct: 68 NLKVYAKSRVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFVRKRNAKYTVNIE 127
Query: 295 VSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 474
V SL L ++ L+L WG++R+DSS + P LD ST + ++ TPFTQ S
Sbjct: 128 VPSLYLSSSDRRLVLRWGIYRADSS-CFVP------LDFKSSTPNDTTTTLETPFTQTSS 180
Query: 475 GTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 654
G LEF++ Q+P +D I++HRKTNFCVPVG G P PLG +
Sbjct: 181 GRFTFELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLT 240
Query: 655 KS 660
S
Sbjct: 241 FS 242
>ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber]
ref|XP_023886632.1| isoamylase 2, chloroplastic [Quercus suber]
gb|POE68275.1| isoamylase 2, chloroplastic [Quercus suber]
Length = 887
Score = 129 bits (324), Expect = 1e-30
Identities = 74/182 (40%), Positives = 100/182 (54%)
Frame = +1
Query: 115 NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 294
N ++ S E+I+ + A+ ++ +YLF TE+GG VKV V ++KY VNIE
Sbjct: 68 NSRVFATSRVLVEEIEQKVSTITEADNREKALTYLFWTEVGGQVKVFVRKTSVKYAVNIE 127
Query: 295 VSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 474
VSSL L +E LI SWG +R+DSS D Q+S+ D+ TS+ TPF Q S
Sbjct: 128 VSSLQLQSSEDRLISSWGRYRADSSSFIPLDVQSSVPDSRTPTSE-------TPFIQTSA 180
Query: 475 GTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 654
G A+ LEF++ Q+P + S I++HRKT FCVPVG G P PLG S
Sbjct: 181 GRFALELEFEAKQIPFYLSFILKLPSDSGSRGSEIRSHRKTKFCVPVGFRKGYPAPLGLS 240
Query: 655 KS 660
S
Sbjct: 241 FS 242
>ref|XP_020592870.1| isoamylase 2, chloroplastic [Phalaenopsis equestris]
Length = 861
Score = 128 bits (321), Expect = 3e-30
Identities = 80/170 (47%), Positives = 94/170 (55%)
Frame = +1
Query: 151 EQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHN 330
E+ QP L EK + F +LFRTE GGLVKV VGA MKY V IEV SLP + HN
Sbjct: 78 EEAQPML------EKQHKGFKFLFRTEFGGLVKVFVGAMEMKYTVCIEVHSLPQSMINHN 131
Query: 331 LILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSA 510
LIL WGMFRSDSS DS+ S+ +S++TPF S L+FD +
Sbjct: 132 LILLWGMFRSDSSVFQGSDSRISV------------SSMKTPFVGES-----CRLDFDHS 174
Query: 511 QVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKS 660
VP +S I+THRKTNFCVPVG+ G PMPLG S S
Sbjct: 175 NVP-FYLSFLLLQPDDTGFESAIRTHRKTNFCVPVGLGPGHPMPLGVSLS 223
>gb|EXB39337.1| Isoamylase 2 [Morus notabilis]
Length = 825
Score = 127 bits (319), Expect = 5e-30
Identities = 70/182 (38%), Positives = 100/182 (54%)
Frame = +1
Query: 115 NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 294
+V+++ S S E +Q L N E+ ++V +YLFRTE+GGLV V V + V +E
Sbjct: 20 SVEVNATSRVSIEPVQKRLSRNTKVEEAEKVSTYLFRTEVGGLVNVFVRKKKVNCTVYVE 79
Query: 295 VSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 474
VSSL L ++ L LSWG++RSDSS +TS + TPFT+NS
Sbjct: 80 VSSLELPRSDGTLGLSWGIYRSDSSSFLPSHFETS-------------TPVETPFTKNSF 126
Query: 475 GTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 654
G + + EF++ ++P ++ I++HRKTNFCVP+G SSG P PLG S
Sbjct: 127 GRYTVEFEFEAKEIPCYLSFLLKSPRDNDSSGLDIRSHRKTNFCVPLGFSSGHPTPLGLS 186
Query: 655 KS 660
S
Sbjct: 187 FS 188
>ref|XP_010090367.2| isoamylase 2, chloroplastic isoform X2 [Morus notabilis]
Length = 836
Score = 127 bits (319), Expect = 5e-30
Identities = 70/182 (38%), Positives = 100/182 (54%)
Frame = +1
Query: 115 NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 294
+V+++ S S E +Q L N E+ ++V +YLFRTE+GGLV V V + V +E
Sbjct: 31 SVEVNATSRVSIEPVQKRLSRNTKVEEAEKVSTYLFRTEVGGLVNVFVRKKKVNCTVYVE 90
Query: 295 VSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 474
VSSL L ++ L LSWG++RSDSS +TS + TPFT+NS
Sbjct: 91 VSSLELPRSDGTLGLSWGIYRSDSSSFLPSHFETS-------------TPVETPFTKNSF 137
Query: 475 GTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 654
G + + EF++ ++P ++ I++HRKTNFCVP+G SSG P PLG S
Sbjct: 138 GRYTVEFEFEAKEIPCYLSFLLKSPRDNDSSGLDIRSHRKTNFCVPLGFSSGHPTPLGLS 197
Query: 655 KS 660
S
Sbjct: 198 FS 199
>ref|XP_024017355.1| isoamylase 2, chloroplastic isoform X1 [Morus notabilis]
Length = 868
Score = 127 bits (319), Expect = 5e-30
Identities = 70/182 (38%), Positives = 100/182 (54%)
Frame = +1
Query: 115 NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 294
+V+++ S S E +Q L N E+ ++V +YLFRTE+GGLV V V + V +E
Sbjct: 63 SVEVNATSRVSIEPVQKRLSRNTKVEEAEKVSTYLFRTEVGGLVNVFVRKKKVNCTVYVE 122
Query: 295 VSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 474
VSSL L ++ L LSWG++RSDSS +TS + TPFT+NS
Sbjct: 123 VSSLELPRSDGTLGLSWGIYRSDSSSFLPSHFETS-------------TPVETPFTKNSF 169
Query: 475 GTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 654
G + + EF++ ++P ++ I++HRKTNFCVP+G SSG P PLG S
Sbjct: 170 GRYTVEFEFEAKEIPCYLSFLLKSPRDNDSSGLDIRSHRKTNFCVPLGFSSGHPTPLGLS 229
Query: 655 KS 660
S
Sbjct: 230 FS 231
>ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]
gb|ONH93660.1| hypothetical protein PRUPE_8G244800 [Prunus persica]
Length = 883
Score = 127 bits (319), Expect = 5e-30
Identities = 72/197 (36%), Positives = 104/197 (52%)
Frame = +1
Query: 70 RKFLEDTTIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVK 249
R+ +++ + + N+K+ S S E ++ E++ +V +YLFRTE+G +V
Sbjct: 53 REVVQNINENLPRDHNLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVN 112
Query: 250 VLVGANNMKYVVNIEVSSLPLYLNEHNLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSD 429
V V N KY VNIEV S L N+ L+L WGM+R+DSS + P LD ST +
Sbjct: 113 VFVRKRNAKYTVNIEVPSSHLSSNDRRLVLRWGMYRADSS-CFVP------LDFKSSTPN 165
Query: 430 VGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXXATDSGIKTHRKTNFCV 609
++ TP Q S G + LEF++ Q+P +D I++HRKTNFCV
Sbjct: 166 DTTTTLETPLIQTSSGRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCV 225
Query: 610 PVGISSGRPMPLGFSKS 660
PVG G P PLG + S
Sbjct: 226 PVGFGRGYPGPLGLTFS 242