BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon25_contig00053245
         (449 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|EXX62227.1| Vac8p [Rhizophagus irregularis DAOM 197198w]           268   2e-85
gb|EXX62226.1| Vac8p [Rhizophagus irregularis DAOM 197198w] >gi|...   268   7e-85
gb|EXX62228.1| Vac8p [Rhizophagus irregularis DAOM 197198w]           261   2e-83
gb|KFH67638.1| vacuolar protein 8 [Mortierella verticillata NRRL...   244   9e-76
gb|OAQ31639.1| vacuolar protein 8 [Mortierella elongata AG-77]        243   3e-75
ref|XP_001730157.1| hypothetical protein MGL_2539 [Malassezia gl...   239   3e-75
ref|XP_021885967.1| putative VAC8-vacuolar membrane protein requ...   243   5e-75
ref|XP_017990314.1| vacuolar protein 8 [Malassezia pachydermatis...   242   1e-74
emb|SHO78279.1| Phosphorylated and palmitoylated vacuolar membra...   240   3e-74
ref|XP_013245360.1| putative VAC8-vacuolar membrane protein requ...   240   4e-74
ref|XP_018740809.1| uncharacterized protein MSY001_2274 [Malasse...   240   4e-74
emb|CEH18179.1| vacuolar protein 8 [Ceraceosorus bombacis]            240   7e-74
ref|XP_007878087.1| hypothetical protein PFL1_02384 [Anthracocys...   239   1e-73
emb|CCF54540.1| probable VAC8-vacuolar membrane protein, require...   238   2e-73
ref|XP_012190698.1| hypothetical protein PHSY_004695 [Pseudozyma...   238   2e-73
emb|SAM77178.1| probable VAC8-vacuolar membrane protein, require...   238   3e-73
ref|XP_014658906.1| vacuolar protein 8 [Moesziomyces antarcticus...   238   3e-73
emb|SJX61225.1| probable VAC8-vacuolar membrane protein, require...   238   3e-73
emb|CDU24424.1| probable VAC8-vacuolar membrane protein, require...   238   3e-73
ref|XP_016291243.1| cytosolic sorting protein GGA2/TOM1 [Kalmano...   238   3e-73

>gb|EXX62227.1| Vac8p [Rhizophagus irregularis DAOM 197198w]
          Length = 509

 Score =  268 bits (684), Expect = 2e-85
 Identities = 139/149 (93%), Positives = 146/149 (97%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA
Sbjct: 307 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 366

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNKRAIVDAGAVER+RELVL+VPLSVQSEMTACVAVLALSDELKP+LLKL IC+VLL
Sbjct: 367 SSERNKRAIVDAGAVERVRELVLNVPLSVQSEMTACVAVLALSDELKPRLLKLGICQVLL 426

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDYT 1
           PLT S SVEVQGNSAAALGNLSSK++DY+
Sbjct: 427 PLTNSSSVEVQGNSAAALGNLSSKVQDYS 455



 Score = 62.8 bits (151), Expect = 1e-08
 Identities = 41/132 (31%), Positives = 68/132 (51%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   E NK  I 
Sbjct: 110 AALGNLAVNTENKLLIVQMGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDE-NKSKIA 167

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  + +L  S  + VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 168 RSGALVPLTKLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLNSPDTDV 227

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 228 QYYCTTALSNIA 239


>gb|EXX62226.1| Vac8p [Rhizophagus irregularis DAOM 197198w]
 gb|PKC15226.1| putative VAC8-vacuolar membrane protein required for the
           cytoplasm-to-vacuole targeting [Rhizophagus irregularis]
 gb|PKC58926.1| putative VAC8-vacuolar membrane protein required for the
           cytoplasm-to-vacuole targeting [Rhizophagus irregularis]
 gb|PKK71313.1| putative VAC8-vacuolar membrane protein required for the
           cytoplasm-to-vacuole targeting [Rhizophagus irregularis]
 gb|PKY29928.1| putative VAC8-vacuolar membrane protein required for the
           cytoplasm-to-vacuole targeting [Rhizophagus irregularis]
 gb|PKY39186.1| putative VAC8-vacuolar membrane protein required for the
           cytoplasm-to-vacuole targeting [Rhizophagus irregularis]
 gb|POG64333.1| vacuolar protein 8 [Rhizophagus irregularis DAOM 181602=DAOM
           197198]
          Length = 553

 Score =  268 bits (684), Expect = 7e-85
 Identities = 139/149 (93%), Positives = 146/149 (97%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA
Sbjct: 307 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 366

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNKRAIVDAGAVER+RELVL+VPLSVQSEMTACVAVLALSDELKP+LLKL IC+VLL
Sbjct: 367 SSERNKRAIVDAGAVERVRELVLNVPLSVQSEMTACVAVLALSDELKPRLLKLGICQVLL 426

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDYT 1
           PLT S SVEVQGNSAAALGNLSSK++DY+
Sbjct: 427 PLTNSSSVEVQGNSAAALGNLSSKVQDYS 455



 Score = 62.8 bits (151), Expect = 1e-08
 Identities = 41/132 (31%), Positives = 68/132 (51%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   E NK  I 
Sbjct: 110 AALGNLAVNTENKLLIVQMGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDE-NKSKIA 167

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  + +L  S  + VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 168 RSGALVPLTKLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLNSPDTDV 227

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 228 QYYCTTALSNIA 239


>gb|EXX62228.1| Vac8p [Rhizophagus irregularis DAOM 197198w]
          Length = 452

 Score =  261 bits (666), Expect = 2e-83
 Identities = 137/144 (95%), Positives = 141/144 (97%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA
Sbjct: 307 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 366

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNKRAIVDAGAVER+RELVL+VPLSVQSEMTACVAVLALSDELKP+LLKL IC+VLL
Sbjct: 367 SSERNKRAIVDAGAVERVRELVLNVPLSVQSEMTACVAVLALSDELKPRLLKLGICQVLL 426

Query: 87  PLTTSRSVEVQGNSAAALGNLSSK 16
           PLT S SVEVQGNSAAALGNLSSK
Sbjct: 427 PLTNSSSVEVQGNSAAALGNLSSK 450



 Score = 62.8 bits (151), Expect = 1e-08
 Identities = 41/132 (31%), Positives = 68/132 (51%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   E NK  I 
Sbjct: 110 AALGNLAVNTENKLLIVQMGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDE-NKSKIA 167

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  + +L  S  + VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 168 RSGALVPLTKLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLNSPDTDV 227

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 228 QYYCTTALSNIA 239


>gb|KFH67638.1| vacuolar protein 8 [Mortierella verticillata NRRL 6337]
          Length = 555

 Score =  244 bits (623), Expect = 9e-76
 Identities = 125/148 (84%), Positives = 140/148 (94%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           +FLPLILSSVACIRNISIHPLNESPIIDAGFL PLI LLAY+ENEEIQCHAISTLRNLAA
Sbjct: 305 TFLPLILSSVACIRNISIHPLNESPIIDAGFLEPLIDLLAYDENEEIQCHAISTLRNLAA 364

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK+AIV+AGAVER+ +LVL+VPLSVQSEMTACVAVLALSD+LKP+LL+L   +VL+
Sbjct: 365 SSERNKKAIVEAGAVERVCDLVLNVPLSVQSEMTACVAVLALSDDLKPRLLQLKALEVLI 424

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAA+GNLSSK++DY
Sbjct: 425 PLTDSSSVEVQGNSAAAIGNLSSKVQDY 452



 Score = 63.5 bits (153), Expect = 8e-09
 Identities = 41/132 (31%), Positives = 68/132 (51%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   E NK  I 
Sbjct: 108 AALGNLAVNTDNKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDE-NKTKIA 165

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S  + VQ   T  +  +  SDE + +L+      VL+ L +S   +V
Sbjct: 166 KSGALVNLTRLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVSLLSSPDTDV 225

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 226 QYYCTTALSNIA 237


>gb|OAQ31639.1| vacuolar protein 8 [Mortierella elongata AG-77]
          Length = 556

 Score =  243 bits (620), Expect = 3e-75
 Identities = 125/148 (84%), Positives = 139/148 (93%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           +FLPLILSSVACIRNISIHPLNESPIIDAGFL PLI+LLAY+ENEEIQCHAISTLRNLAA
Sbjct: 305 TFLPLILSSVACIRNISIHPLNESPIIDAGFLDPLIELLAYDENEEIQCHAISTLRNLAA 364

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK+AIV+AGAVER+  LVL+VPLSVQSEMTACVAVLALSD+LKP+LL+L    VL+
Sbjct: 365 SSERNKKAIVEAGAVERVCALVLNVPLSVQSEMTACVAVLALSDDLKPRLLQLGALDVLI 424

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAA+GNLSSK++DY
Sbjct: 425 PLTDSSSVEVQGNSAAAIGNLSSKVQDY 452



 Score = 63.2 bits (152), Expect = 1e-08
 Identities = 41/132 (31%), Positives = 68/132 (51%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   E NK  I 
Sbjct: 108 AALGNLAVNTENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDE-NKTKIA 165

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S  + VQ   T  +  +  SDE + +L+      VL+ L +S   +V
Sbjct: 166 KSGALVPLTRLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVSLLSSPDTDV 225

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 226 QYYCTTALSNIA 237



 Score = 55.8 bits (133), Expect = 4e-06
 Identities = 40/116 (34%), Positives = 59/116 (50%)
 Frame = -3

Query: 369 IDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIVDAGAVERIRELVLSVP 190
           +D   L P++ LL    + E+Q  A + L NLA ++E NK  IV  G +E +   +LS  
Sbjct: 83  VDRDTLEPVLFLLQ-SHDVEVQRAASAALGNLAVNTE-NKLLIVKLGGLEPLIRQMLSPN 140

Query: 189 LSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEVQGNSAAALGNLS 22
           + VQ     C+  LA  DE K K+ K      L  L  S+ + VQ N+  AL N++
Sbjct: 141 VEVQCNAVGCITNLATHDENKTKIAKSGALVPLTRLARSKDMRVQRNATGALLNMT 196


>ref|XP_001730157.1| hypothetical protein MGL_2539 [Malassezia globosa CBS 7966]
 gb|EDP42943.1| hypothetical protein MGL_2539 [Malassezia globosa CBS 7966]
          Length = 435

 Score =  239 bits (611), Expect = 3e-75
 Identities = 120/149 (80%), Positives = 138/149 (92%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP+NESPIIDAGFL PLI+LL++EENEE+QCHAISTLRNLAA
Sbjct: 170 SFLPLILSAAACVRNVSIHPMNESPIIDAGFLHPLIELLSHEENEELQCHAISTLRNLAA 229

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AI+DAGAVERI++LVL VPLSVQSEMTAC AVLALS++LKP+LL + IC+VLL
Sbjct: 230 SSERNKAAIIDAGAVERIKDLVLHVPLSVQSEMTACTAVLALSEDLKPQLLDMGICEVLL 289

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDYT 1
           PLT S S+EVQGNSAAALGNLSSK  DY+
Sbjct: 290 PLTDSPSIEVQGNSAAALGNLSSKADDYS 318


>ref|XP_021885967.1| putative VAC8-vacuolar membrane protein required for the
           cytoplasm-to-vacuole targeting [Lobosporangium
           transversale]
 gb|ORZ28282.1| putative VAC8-vacuolar membrane protein required for the
           cytoplasm-to-vacuole targeting [Lobosporangium
           transversale]
          Length = 566

 Score =  243 bits (619), Expect = 5e-75
 Identities = 125/148 (84%), Positives = 138/148 (93%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           ++LPLILSSVACIRNISIHPLNESPIIDAGFL PLI+LLAY+ENEEIQCHAISTLRNLAA
Sbjct: 305 TYLPLILSSVACIRNISIHPLNESPIIDAGFLEPLIELLAYDENEEIQCHAISTLRNLAA 364

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK+AIV+AGAVER+  LVL+VPLSVQSEMTACVAVLALSDELKP+LL+L    VL+
Sbjct: 365 SSERNKKAIVEAGAVERVCTLVLNVPLSVQSEMTACVAVLALSDELKPRLLQLGALNVLI 424

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAA+GNLSSK+ DY
Sbjct: 425 PLTESPSVEVQGNSAAAIGNLSSKVEDY 452



 Score = 66.2 bits (160), Expect = 9e-10
 Identities = 43/132 (32%), Positives = 68/132 (51%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++P N+  I+  G L PLI+ +    N E+QC+A+  + NLA   E NK  I 
Sbjct: 108 AALGNLAVNPENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDE-NKTKIA 165

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S    VQ   T  +  +  SDE + +L+      VL+ L TS   +V
Sbjct: 166 KSGALVPLTRLAKSKDKRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVNLLTSSDTDV 225

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 226 QYYCTTALSNIA 237


>ref|XP_017990314.1| vacuolar protein 8 [Malassezia pachydermatis]
 gb|KOS12682.1| vacuolar protein 8 [Malassezia pachydermatis]
          Length = 568

 Score =  242 bits (617), Expect = 1e-74
 Identities = 122/149 (81%), Positives = 138/149 (92%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP+NESPII+AGFL PLI+LL++EENEE+QCHAISTLRNLAA
Sbjct: 306 SFLPLILSAAACVRNVSIHPMNESPIIEAGFLHPLIELLSHEENEELQCHAISTLRNLAA 365

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AI+DAGAVERI+ELVL VPLSVQSEMTAC AVLALS++LKP+LL + IC+VLL
Sbjct: 366 SSERNKAAIIDAGAVERIKELVLHVPLSVQSEMTACTAVLALSEDLKPQLLDMGICEVLL 425

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDYT 1
           PLT S SVEVQGNSAAALGNLSSK  DYT
Sbjct: 426 PLTDSPSVEVQGNSAAALGNLSSKAEDYT 454



 Score = 62.0 bits (149), Expect = 3e-08
 Identities = 41/135 (30%), Positives = 68/135 (50%)
 Frame = -3

Query: 426 SSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKR 247
           +S A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   E NK 
Sbjct: 106 ASSAALGNLAVNQENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDE-NKT 163

Query: 246 AIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRS 67
            I  +GA+  +  L  S  + VQ      +  +  SDE + +L+      VL+ L  S  
Sbjct: 164 KIAKSGALVPLTRLARSKDIRVQRNAAGALLNMTHSDENRQQLVNAGAISVLVTLLGSTD 223

Query: 66  VEVQGNSAAALGNLS 22
            +VQ     AL N++
Sbjct: 224 TDVQYYCTTALSNIA 238


>emb|SHO78279.1| Phosphorylated and palmitoylated vacuolar membrane protein
           [Malassezia sympodialis ATCC 42132]
          Length = 562

 Score =  240 bits (613), Expect = 3e-74
 Identities = 121/148 (81%), Positives = 137/148 (92%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP+NESPIIDAGFL PLI+LL++EENEE+QCHAISTLRNLAA
Sbjct: 304 SFLPLILSAAACVRNVSIHPMNESPIIDAGFLHPLIELLSHEENEELQCHAISTLRNLAA 363

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AI+DAGAVERI+ELVL VPLSVQSEMTAC AVLALS++LKP+LL + IC+VLL
Sbjct: 364 SSERNKAAIIDAGAVERIKELVLHVPLSVQSEMTACAAVLALSEDLKPQLLDMGICEVLL 423

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S S+EVQGNSAAALGNLSSK  DY
Sbjct: 424 PLTDSPSIEVQGNSAAALGNLSSKADDY 451



 Score = 62.0 bits (149), Expect = 3e-08
 Identities = 40/135 (29%), Positives = 69/135 (51%)
 Frame = -3

Query: 426 SSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKR 247
           +S A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   + NK 
Sbjct: 104 ASSAALGNLAVNSENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDD-NKT 161

Query: 246 AIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRS 67
            I  +GA+  +  L  S  + VQ      +  +  SDE + +L+      VL+ L +S  
Sbjct: 162 KIAKSGALVPLTRLARSKDIRVQRNAAGALLNMTHSDENRQQLVNAGAISVLVTLLSSTD 221

Query: 66  VEVQGNSAAALGNLS 22
            +VQ     AL N++
Sbjct: 222 TDVQYYCTTALSNIA 236



 Score = 55.1 bits (131), Expect = 7e-06
 Identities = 39/111 (35%), Positives = 58/111 (52%)
 Frame = -3

Query: 354 LSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIVDAGAVERIRELVLSVPLSVQS 175
           L P++ LL    + E+Q  + + L NLA +SE NK  IV  G +E +   +LS  + VQ 
Sbjct: 87  LEPVLFLLQ-SHDVEVQRASSAALGNLAVNSE-NKLLIVKLGGLEPLIRQMLSPNVEVQC 144

Query: 174 EMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEVQGNSAAALGNLS 22
               C+  LA  D+ K K+ K      L  L  S+ + VQ N+A AL N++
Sbjct: 145 NAVGCITNLATHDDNKTKIAKSGALVPLTRLARSKDIRVQRNAAGALLNMT 195


>ref|XP_013245360.1| putative VAC8-vacuolar membrane protein required for the
           cytoplasm-to-vacuole targeting [Tilletiaria anomala UBC
           951]
 gb|KDN52521.1| putative VAC8-vacuolar membrane protein required for the
           cytoplasm-to-vacuole targeting [Tilletiaria anomala UBC
           951]
          Length = 568

 Score =  240 bits (613), Expect = 4e-74
 Identities = 122/148 (82%), Positives = 138/148 (93%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHPLNESPII+AGFL PLI LL++E+NEEIQCHAISTLRNLAA
Sbjct: 310 SFLPLILSAAACVRNVSIHPLNESPIIEAGFLHPLIDLLSHEDNEEIQCHAISTLRNLAA 369

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AIV+AGAVERI+ELVLSVPLSVQSEMTAC AVLALS++LKP+LL++ IC+VL+
Sbjct: 370 SSERNKSAIVEAGAVERIKELVLSVPLSVQSEMTACAAVLALSEDLKPQLLEMGICEVLI 429

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAALGNLSSK  DY
Sbjct: 430 PLTASPSVEVQGNSAAALGNLSSKSEDY 457



 Score = 62.0 bits (149), Expect = 3e-08
 Identities = 40/132 (30%), Positives = 68/132 (51%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   + NK  I 
Sbjct: 113 AALGNLAVNAENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDD-NKTKIA 170

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S  + VQ   T  +  +  SDE + +L+      VL+ L +S   +V
Sbjct: 171 KSGALVPLTRLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLSSSDTDV 230

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 231 QYYCTTALSNIA 242


>ref|XP_018740809.1| uncharacterized protein MSY001_2274 [Malassezia sympodialis ATCC
           42132]
 emb|CCU99568.1| unnamed protein product [Malassezia sympodialis ATCC 42132]
          Length = 570

 Score =  240 bits (613), Expect = 4e-74
 Identities = 121/148 (81%), Positives = 137/148 (92%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP+NESPIIDAGFL PLI+LL++EENEE+QCHAISTLRNLAA
Sbjct: 312 SFLPLILSAAACVRNVSIHPMNESPIIDAGFLHPLIELLSHEENEELQCHAISTLRNLAA 371

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AI+DAGAVERI+ELVL VPLSVQSEMTAC AVLALS++LKP+LL + IC+VLL
Sbjct: 372 SSERNKAAIIDAGAVERIKELVLHVPLSVQSEMTACAAVLALSEDLKPQLLDMGICEVLL 431

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S S+EVQGNSAAALGNLSSK  DY
Sbjct: 432 PLTDSPSIEVQGNSAAALGNLSSKADDY 459



 Score = 62.0 bits (149), Expect = 3e-08
 Identities = 40/135 (29%), Positives = 69/135 (51%)
 Frame = -3

Query: 426 SSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKR 247
           +S A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   + NK 
Sbjct: 112 ASSAALGNLAVNSENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDD-NKT 169

Query: 246 AIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRS 67
            I  +GA+  +  L  S  + VQ      +  +  SDE + +L+      VL+ L +S  
Sbjct: 170 KIAKSGALVPLTRLARSKDIRVQRNAAGALLNMTHSDENRQQLVNAGAISVLVTLLSSTD 229

Query: 66  VEVQGNSAAALGNLS 22
            +VQ     AL N++
Sbjct: 230 TDVQYYCTTALSNIA 244



 Score = 55.1 bits (131), Expect = 7e-06
 Identities = 39/111 (35%), Positives = 58/111 (52%)
 Frame = -3

Query: 354 LSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIVDAGAVERIRELVLSVPLSVQS 175
           L P++ LL    + E+Q  + + L NLA +SE NK  IV  G +E +   +LS  + VQ 
Sbjct: 95  LEPVLFLLQ-SHDVEVQRASSAALGNLAVNSE-NKLLIVKLGGLEPLIRQMLSPNVEVQC 152

Query: 174 EMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEVQGNSAAALGNLS 22
               C+  LA  D+ K K+ K      L  L  S+ + VQ N+A AL N++
Sbjct: 153 NAVGCITNLATHDDNKTKIAKSGALVPLTRLARSKDIRVQRNAAGALLNMT 203


>emb|CEH18179.1| vacuolar protein 8 [Ceraceosorus bombacis]
          Length = 579

 Score =  240 bits (612), Expect = 7e-74
 Identities = 122/148 (82%), Positives = 138/148 (93%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHPLNESPIIDAGFL PLI LL++E+NEEIQCHAISTLRNLAA
Sbjct: 305 SFLPLILSAAACVRNVSIHPLNESPIIDAGFLHPLIDLLSHEDNEEIQCHAISTLRNLAA 364

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AIV+AGAVERI+ELVL+VPLSVQSEMTAC AVLALS++LKP+LL++ IC+VL+
Sbjct: 365 SSERNKTAIVEAGAVERIKELVLNVPLSVQSEMTACAAVLALSEDLKPQLLEMGICEVLI 424

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAALGNLSSK  DY
Sbjct: 425 PLTASPSVEVQGNSAAALGNLSSKSDDY 452



 Score = 62.0 bits (149), Expect = 3e-08
 Identities = 41/132 (31%), Positives = 67/132 (50%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   E NK  I 
Sbjct: 108 AALGNLAVNSENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDE-NKTKIA 165

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S  + VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 166 KSGALVPLTRLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLGSSDTDV 225

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 226 QYYCTTALSNIA 237



 Score = 55.1 bits (131), Expect = 7e-06
 Identities = 38/107 (35%), Positives = 53/107 (49%)
 Frame = -3

Query: 342 IQLLAYEENEEIQCHAISTLRNLAASSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTA 163
           I  L    + E+Q  A + L NLA +SE NK  IV  G +E +   +LS  + VQ     
Sbjct: 91  IMFLLQSHDVEVQRAASAALGNLAVNSE-NKLLIVKLGGLEPLIRQMLSPNVEVQCNAVG 149

Query: 162 CVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEVQGNSAAALGNLS 22
           C+  LA  DE K K+ K      L  L  S+ + VQ N+  AL N++
Sbjct: 150 CITNLATHDENKTKIAKSGALVPLTRLARSKDMRVQRNATGALLNMT 196


>ref|XP_007878087.1| hypothetical protein PFL1_02384 [Anthracocystis flocculosa PF-1]
 gb|EPQ30268.1| hypothetical protein PFL1_02384 [Anthracocystis flocculosa PF-1]
          Length = 568

 Score =  239 bits (610), Expect = 1e-73
 Identities = 120/148 (81%), Positives = 138/148 (93%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP+NESPIIDAGFL PLI LL++E+NEEIQCHAISTLRNLAA
Sbjct: 307 SFLPLILSAAACVRNVSIHPMNESPIIDAGFLHPLIDLLSHEDNEEIQCHAISTLRNLAA 366

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AIV+AGAVERI+ELVL+VPLSVQSEMTAC AVLALS++LKP+LL++ IC+VL+
Sbjct: 367 SSERNKTAIVEAGAVERIKELVLNVPLSVQSEMTACAAVLALSEDLKPQLLEMGICEVLI 426

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S S+EVQGNSAAALGNLSSK  DY
Sbjct: 427 PLTASSSIEVQGNSAAALGNLSSKSDDY 454



 Score = 59.3 bits (142), Expect = 2e-07
 Identities = 40/132 (30%), Positives = 66/132 (50%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   + NK  I 
Sbjct: 110 AALGNLAVNAENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDD-NKTKIA 167

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S    VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 168 KSGALVPLTRLARSKDQRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLGSSDTDV 227

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 228 QYYCTTALSNIA 239


>emb|CCF54540.1| probable VAC8-vacuolar membrane protein, required for the
           cytoplasm-to-vacuole targeting [Ustilago hordei]
          Length = 561

 Score =  238 bits (608), Expect = 2e-73
 Identities = 121/148 (81%), Positives = 137/148 (92%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP NESPIIDAGFL PLI LL++E+NEEIQCHAISTLRNLAA
Sbjct: 306 SFLPLILSAAACVRNVSIHPANESPIIDAGFLHPLIDLLSHEDNEEIQCHAISTLRNLAA 365

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AIV+AGAVERI+ELVL+VPLSVQSEMTAC AVLALS++LKP+LL++ IC+VL+
Sbjct: 366 SSERNKTAIVEAGAVERIKELVLNVPLSVQSEMTACAAVLALSEDLKPQLLEMGICEVLI 425

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAALGNLSSK  DY
Sbjct: 426 PLTASSSVEVQGNSAAALGNLSSKSDDY 453



 Score = 60.8 bits (146), Expect = 7e-08
 Identities = 40/132 (30%), Positives = 67/132 (50%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   + NK  I 
Sbjct: 109 AALGNLAVNAENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDD-NKTKIA 166

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S  + VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 167 KSGALVPLTRLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLGSSDTDV 226

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 227 QYYCTTALSNIA 238


>ref|XP_012190698.1| hypothetical protein PHSY_004695 [Pseudozyma hubeiensis SY62]
 dbj|GAC97111.1| hypothetical protein PHSY_004695 [Pseudozyma hubeiensis SY62]
          Length = 559

 Score =  238 bits (607), Expect = 2e-73
 Identities = 121/148 (81%), Positives = 137/148 (92%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP NESPIIDAGFL PLI LL++E+NEEIQCHAISTLRNLAA
Sbjct: 303 SFLPLILSAAACVRNVSIHPANESPIIDAGFLHPLIDLLSHEDNEEIQCHAISTLRNLAA 362

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AIV+AGAVERI+ELVL+VPLSVQSEMTAC AVLALS++LKP+LL++ IC+VL+
Sbjct: 363 SSERNKTAIVEAGAVERIKELVLNVPLSVQSEMTACAAVLALSEDLKPQLLEMGICEVLI 422

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAALGNLSSK  DY
Sbjct: 423 PLTASPSVEVQGNSAAALGNLSSKSDDY 450



 Score = 60.8 bits (146), Expect = 7e-08
 Identities = 40/132 (30%), Positives = 67/132 (50%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   + NK  I 
Sbjct: 106 AALGNLAVNAENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDD-NKTKIA 163

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S  + VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 164 KSGALVPLTRLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLGSSDTDV 223

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 224 QYYCTTALSNIA 235


>emb|SAM77178.1| probable VAC8-vacuolar membrane protein, required for the
           cytoplasm-to-vacuole targeting [Ustilago bromivora]
          Length = 561

 Score =  238 bits (607), Expect = 3e-73
 Identities = 121/148 (81%), Positives = 137/148 (92%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP NESPIIDAGFL PLI LL++E+NEEIQCHAISTLRNLAA
Sbjct: 306 SFLPLILSAAACVRNVSIHPANESPIIDAGFLHPLIDLLSHEDNEEIQCHAISTLRNLAA 365

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AIV+AGAVERI+ELVL+VPLSVQSEMTAC AVLALS++LKP+LL++ IC+VL+
Sbjct: 366 SSERNKTAIVEAGAVERIKELVLNVPLSVQSEMTACAAVLALSEDLKPQLLEMGICEVLI 425

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAALGNLSSK  DY
Sbjct: 426 PLTASPSVEVQGNSAAALGNLSSKSDDY 453



 Score = 60.8 bits (146), Expect = 7e-08
 Identities = 40/132 (30%), Positives = 67/132 (50%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   + NK  I 
Sbjct: 109 AALGNLAVNAENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDD-NKTKIA 166

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S  + VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 167 KSGALVPLTRLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLGSSDTDV 226

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 227 QYYCTTALSNIA 238


>ref|XP_014658906.1| vacuolar protein 8 [Moesziomyces antarcticus]
 dbj|GAK63051.1| vacuolar protein 8 [Moesziomyces antarcticus]
          Length = 561

 Score =  238 bits (607), Expect = 3e-73
 Identities = 121/148 (81%), Positives = 137/148 (92%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP NESPIIDAGFL PLI LL++E+NEEIQCHAISTLRNLAA
Sbjct: 306 SFLPLILSAAACVRNVSIHPANESPIIDAGFLHPLIDLLSHEDNEEIQCHAISTLRNLAA 365

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AIV+AGAVERI+ELVL+VPLSVQSEMTAC AVLALS++LKP+LL++ IC+VL+
Sbjct: 366 SSERNKTAIVEAGAVERIKELVLNVPLSVQSEMTACAAVLALSEDLKPQLLEMGICEVLI 425

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAALGNLSSK  DY
Sbjct: 426 PLTASPSVEVQGNSAAALGNLSSKSDDY 453



 Score = 60.8 bits (146), Expect = 7e-08
 Identities = 40/132 (30%), Positives = 67/132 (50%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   + NK  I 
Sbjct: 109 AALGNLAVNAENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDD-NKTKIA 166

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S  + VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 167 KSGALVPLTRLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLGSSDTDV 226

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 227 QYYCTTALSNIA 238


>emb|SJX61225.1| probable VAC8-vacuolar membrane protein, required for the
           cytoplasm-to-vacuole targeting [Sporisorium reilianum f.
           sp. reilianum]
          Length = 563

 Score =  238 bits (607), Expect = 3e-73
 Identities = 121/148 (81%), Positives = 137/148 (92%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP NESPIIDAGFL PLI LL++E+NEEIQCHAISTLRNLAA
Sbjct: 306 SFLPLILSAAACVRNVSIHPANESPIIDAGFLHPLIDLLSHEDNEEIQCHAISTLRNLAA 365

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AIV+AGAVERI+ELVL+VPLSVQSEMTAC AVLALS++LKP+LL++ IC+VL+
Sbjct: 366 SSERNKTAIVEAGAVERIKELVLNVPLSVQSEMTACAAVLALSEDLKPQLLEMGICEVLI 425

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAALGNLSSK  DY
Sbjct: 426 PLTASPSVEVQGNSAAALGNLSSKSDDY 453



 Score = 60.8 bits (146), Expect = 7e-08
 Identities = 40/132 (30%), Positives = 67/132 (50%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   + NK  I 
Sbjct: 109 AALGNLAVNAENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDD-NKTKIA 166

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S  + VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 167 KSGALVPLTRLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLGSSDTDV 226

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 227 QYYCTTALSNIA 238


>emb|CDU24424.1| probable VAC8-vacuolar membrane protein, required for the
           cytoplasm-to-vacuole targeting [Sporisorium scitamineum]
          Length = 563

 Score =  238 bits (607), Expect = 3e-73
 Identities = 121/148 (81%), Positives = 137/148 (92%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP NESPIIDAGFL PLI LL++E+NEEIQCHAISTLRNLAA
Sbjct: 306 SFLPLILSAAACVRNVSIHPANESPIIDAGFLHPLIDLLSHEDNEEIQCHAISTLRNLAA 365

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AIV+AGAVERI+ELVL+VPLSVQSEMTAC AVLALS++LKP+LL++ IC+VL+
Sbjct: 366 SSERNKTAIVEAGAVERIKELVLNVPLSVQSEMTACAAVLALSEDLKPQLLEMGICEVLI 425

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAALGNLSSK  DY
Sbjct: 426 PLTASPSVEVQGNSAAALGNLSSKSDDY 453



 Score = 60.8 bits (146), Expect = 7e-08
 Identities = 40/132 (30%), Positives = 67/132 (50%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   + NK  I 
Sbjct: 109 AALGNLAVNAENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDD-NKTKIA 166

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S  + VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 167 KSGALVPLTRLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLGSSDTDV 226

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 227 QYYCTTALSNIA 238


>ref|XP_016291243.1| cytosolic sorting protein GGA2/TOM1 [Kalmanozyma brasiliensis
           GHG001]
 gb|EST06254.1| cytosolic sorting protein GGA2/TOM1 [Kalmanozyma brasiliensis
           GHG001]
          Length = 563

 Score =  238 bits (607), Expect = 3e-73
 Identities = 121/148 (81%), Positives = 137/148 (92%)
 Frame = -3

Query: 447 SFLPLILSSVACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAA 268
           SFLPLILS+ AC+RN+SIHP NESPIIDAGFL PLI LL++E+NEEIQCHAISTLRNLAA
Sbjct: 306 SFLPLILSAAACVRNVSIHPANESPIIDAGFLHPLIDLLSHEDNEEIQCHAISTLRNLAA 365

Query: 267 SSERNKRAIVDAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLL 88
           SSERNK AIV+AGAVERI+ELVL+VPLSVQSEMTAC AVLALS++LKP+LL++ IC+VL+
Sbjct: 366 SSERNKTAIVEAGAVERIKELVLNVPLSVQSEMTACAAVLALSEDLKPQLLEMGICEVLI 425

Query: 87  PLTTSRSVEVQGNSAAALGNLSSKIRDY 4
           PLT S SVEVQGNSAAALGNLSSK  DY
Sbjct: 426 PLTASPSVEVQGNSAAALGNLSSKSDDY 453



 Score = 60.8 bits (146), Expect = 7e-08
 Identities = 40/132 (30%), Positives = 67/132 (50%)
 Frame = -3

Query: 417 ACIRNISIHPLNESPIIDAGFLSPLIQLLAYEENEEIQCHAISTLRNLAASSERNKRAIV 238
           A + N++++  N+  I+  G L PLI+ +    N E+QC+A+  + NLA   + NK  I 
Sbjct: 109 AALGNLAVNAENKLLIVKLGGLEPLIRQML-SPNVEVQCNAVGCITNLATHDD-NKTKIA 166

Query: 237 DAGAVERIRELVLSVPLSVQSEMTACVAVLALSDELKPKLLKLDICKVLLPLTTSRSVEV 58
            +GA+  +  L  S  + VQ   T  +  +  SDE + +L+      VL+ L  S   +V
Sbjct: 167 KSGALVPLTRLARSKDMRVQRNATGALLNMTHSDENRQQLVNAGAIPVLVGLLGSSDTDV 226

Query: 57  QGNSAAALGNLS 22
           Q     AL N++
Sbjct: 227 QYYCTTALSNIA 238