BLASTX nr result
ID: Ophiopogon25_contig00044435
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon25_contig00044435
(2135 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|PKK61711.1| hypothetical protein RhiirC2_791410 [Rhizophagus ... 256 3e-78
dbj|GBC52755.1| hypothetical protein RIR_3406100 [Rhizophagus ir... 216 6e-63
dbj|GBC51674.1| hypothetical protein: PROVISIONAL [Rhizophagus i... 206 2e-59
gb|EXX55918.1| hypothetical protein RirG_220910 [Rhizophagus irr... 206 2e-59
gb|POG81146.1| hypothetical protein GLOIN_2v1763770 [Rhizophagus... 166 1e-44
gb|PKB92246.1| hypothetical protein RhiirA5_445421, partial [Rhi... 153 1e-40
gb|PKY40191.1| hypothetical protein RhiirA4_538656 [Rhizophagus ... 143 1e-36
gb|PKK61813.1| hypothetical protein RhiirC2_791263 [Rhizophagus ... 144 3e-36
gb|PKK68463.1| hypothetical protein RhiirC2_867249 [Rhizophagus ... 124 6e-30
gb|PKY59596.1| hypothetical protein RhiirA4_482466 [Rhizophagus ... 116 1e-26
gb|PKK58547.1| hypothetical protein RhiirC2_871526 [Rhizophagus ... 115 2e-26
gb|PKC05693.1| hypothetical protein RhiirA5_420589 [Rhizophagus ... 103 3e-22
gb|PKC00700.1| hypothetical protein RhiirA5_382268 [Rhizophagus ... 102 1e-21
gb|PKK57333.1| hypothetical protein RhiirC2_858257 [Rhizophagus ... 100 4e-21
gb|PKB98266.1| hypothetical protein RhiirA5_465269 [Rhizophagus ... 98 3e-20
gb|PKK58473.1| hypothetical protein RhiirC2_857774 [Rhizophagus ... 97 7e-20
gb|PKY54691.1| hypothetical protein RhiirA4_426951 [Rhizophagus ... 95 1e-18
gb|PKY40192.1| hypothetical protein RhiirA4_453508 [Rhizophagus ... 66 5e-18
gb|PKK59138.1| hypothetical protein RhiirC2_762944 [Rhizophagus ... 62 2e-17
gb|EXX62634.1| hypothetical protein RirG_159960 [Rhizophagus irr... 88 2e-17
>gb|PKK61711.1| hypothetical protein RhiirC2_791410 [Rhizophagus irregularis]
Length = 148
Score = 256 bits (654), Expect = 3e-78
Identities = 127/148 (85%), Positives = 131/148 (88%), Gaps = 1/148 (0%)
Frame = -2
Query: 769 MEQTLVIDSDISAGDKPFEVHEFLEALGLLDKEASPDFRYEIXXXXXXXXXXXXXD-NYW 593
MEQTLVIDSDISAGDKPFEVHEFLEALGLLDKEASPDFRYEI NYW
Sbjct: 1 MEQTLVIDSDISAGDKPFEVHEFLEALGLLDKEASPDFRYEIDDVDMSDDDSDDDQGNYW 60
Query: 592 DPRKRYTGWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSR 413
+PRK YTGWDYKRRFGE+ALKKLYQ REARHRNIVLMEFTRRLPDIS++EKNRLFDAFSR
Sbjct: 61 NPRKTYTGWDYKRRFGENALKKLYQAREARHRNIVLMEFTRRLPDISEQEKNRLFDAFSR 120
Query: 412 SNAFGVKEHNALLDALADPVKGWDLNNY 329
SNAFGVKEHNALLDALADPVKGWDLNNY
Sbjct: 121 SNAFGVKEHNALLDALADPVKGWDLNNY 148
>dbj|GBC52755.1| hypothetical protein RIR_3406100 [Rhizophagus irregularis DAOM
181602]
gb|POG68855.1| hypothetical protein GLOIN_2v1878027 [Rhizophagus irregularis DAOM
181602=DAOM 197198]
Length = 146
Score = 216 bits (549), Expect = 6e-63
Identities = 115/150 (76%), Positives = 118/150 (78%), Gaps = 3/150 (2%)
Frame = -2
Query: 769 MEQTLVIDSDISAGDKPFEVHEFLEALGLLDKEASP---DFRYEIXXXXXXXXXXXXXDN 599
MEQT SD SAGDK EV+EFLEALGLLD E +P D DN
Sbjct: 1 MEQTY---SDTSAGDK-IEVYEFLEALGLLDNEVAPVNMDDDDNNESDIDEEDSDDDQDN 56
Query: 598 YWDPRKRYTGWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAF 419
YWDPRKRYTGWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAF
Sbjct: 57 YWDPRKRYTGWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAF 116
Query: 418 SRSNAFGVKEHNALLDALADPVKGWDLNNY 329
SRSN FG KEHNALLDALA+PVKGWDLNNY
Sbjct: 117 SRSNTFGAKEHNALLDALANPVKGWDLNNY 146
>dbj|GBC51674.1| hypothetical protein: PROVISIONAL [Rhizophagus irregularis DAOM
181602]
gb|POG68567.1| hypothetical protein GLOIN_2v1778213 [Rhizophagus irregularis DAOM
181602=DAOM 197198]
Length = 146
Score = 206 bits (525), Expect = 2e-59
Identities = 109/150 (72%), Positives = 116/150 (77%), Gaps = 3/150 (2%)
Frame = -2
Query: 769 MEQTLVIDSDISAGDKPFEVHEFLEALGLLDKEASP---DFRYEIXXXXXXXXXXXXXDN 599
MEQT SD SAGDK EV+EFLEALGLLD E +P D +N
Sbjct: 1 MEQTY---SDTSAGDK-IEVYEFLEALGLLDNEVAPVNMDDDDNSESDIDEEDSDDDQEN 56
Query: 598 YWDPRKRYTGWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAF 419
YWDPRK YTGW+YKRRFGEDALKKLYQTREARHRNIVLMEFTRRLP+IS+ EKNRLFDAF
Sbjct: 57 YWDPRKTYTGWNYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPNISEEEKNRLFDAF 116
Query: 418 SRSNAFGVKEHNALLDALADPVKGWDLNNY 329
SRSNAFG KEHNALLD LADPVKGWDL+NY
Sbjct: 117 SRSNAFGAKEHNALLDTLADPVKGWDLDNY 146
>gb|EXX55918.1| hypothetical protein RirG_220910 [Rhizophagus irregularis DAOM
197198w]
dbj|GBC47072.1| hypothetical protein: PROVISIONAL [Rhizophagus irregularis DAOM
181602]
gb|POG82954.1| hypothetical protein GLOIN_2v1866931 [Rhizophagus irregularis DAOM
181602=DAOM 197198]
Length = 146
Score = 206 bits (524), Expect = 2e-59
Identities = 108/150 (72%), Positives = 115/150 (76%), Gaps = 3/150 (2%)
Frame = -2
Query: 769 MEQTLVIDSDISAGDKPFEVHEFLEALGLLDKEASP---DFRYEIXXXXXXXXXXXXXDN 599
MEQT SD SAGDK E++EFLEALGLLD E SP D N
Sbjct: 1 MEQT---HSDTSAGDK-IEIYEFLEALGLLDNEVSPINMDDDDNNESDIDEEDSDDDQKN 56
Query: 598 YWDPRKRYTGWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAF 419
YWDPRK YTGWDYKRRFGEDALKKLYQTREA H+NIVLMEFTRRLP+IS++EKNRLFDAF
Sbjct: 57 YWDPRKTYTGWDYKRRFGEDALKKLYQTREAHHQNIVLMEFTRRLPNISEKEKNRLFDAF 116
Query: 418 SRSNAFGVKEHNALLDALADPVKGWDLNNY 329
SRSNAFG KEHNALLD LADPVKGWDL+NY
Sbjct: 117 SRSNAFGAKEHNALLDTLADPVKGWDLDNY 146
>gb|POG81146.1| hypothetical protein GLOIN_2v1763770 [Rhizophagus irregularis DAOM
181602=DAOM 197198]
Length = 120
Score = 166 bits (419), Expect = 1e-44
Identities = 97/147 (65%), Positives = 103/147 (70%)
Frame = -2
Query: 769 MEQTLVIDSDISAGDKPFEVHEFLEALGLLDKEASPDFRYEIXXXXXXXXXXXXXDNYWD 590
MEQT SD SAGDK EV+EFLEALGLLD E SP N D
Sbjct: 1 MEQTY---SDTSAGDK-IEVYEFLEALGLLDNEVSP-------------------VNMDD 37
Query: 589 PRKRYTGWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRS 410
+ D + ++ALKKLYQTREARHRNIVLMEFTRRLPDISD+EKNRLFDAFSRS
Sbjct: 38 DDNNESDIDEE----DNALKKLYQTREARHRNIVLMEFTRRLPDISDQEKNRLFDAFSRS 93
Query: 409 NAFGVKEHNALLDALADPVKGWDLNNY 329
NAFGVKEHN LLDALADPVKGWDLNNY
Sbjct: 94 NAFGVKEHNVLLDALADPVKGWDLNNY 120
>gb|PKB92246.1| hypothetical protein RhiirA5_445421, partial [Rhizophagus
irregularis]
Length = 79
Score = 153 bits (386), Expect = 1e-40
Identities = 72/79 (91%), Positives = 77/79 (97%)
Frame = -2
Query: 565 DYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRSNAFGVKEH 386
DYKRRFGEDALKKLYQTREARH+NIVLMEFTRRLP+IS++EKNRLFDAFSRSNAFG KEH
Sbjct: 1 DYKRRFGEDALKKLYQTREARHQNIVLMEFTRRLPNISEKEKNRLFDAFSRSNAFGAKEH 60
Query: 385 NALLDALADPVKGWDLNNY 329
NALLD LADPVKGWDL+NY
Sbjct: 61 NALLDTLADPVKGWDLDNY 79
>gb|PKY40191.1| hypothetical protein RhiirA4_538656 [Rhizophagus irregularis]
Length = 109
Score = 143 bits (360), Expect = 1e-36
Identities = 87/147 (59%), Positives = 92/147 (62%)
Frame = -2
Query: 769 MEQTLVIDSDISAGDKPFEVHEFLEALGLLDKEASPDFRYEIXXXXXXXXXXXXXDNYWD 590
MEQT SD SAGDK EV+EFLEALGLLD E +P N D
Sbjct: 1 MEQTY---SDTSAGDK-IEVYEFLEALGLLDNEVAP-------------------VNMDD 37
Query: 589 PRKRYTGWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRS 410
+D + + RHRNIVLMEFTRRLPDISDREKNRLFDAFSRS
Sbjct: 38 ---------------DDNNESDIDEEDTRHRNIVLMEFTRRLPDISDREKNRLFDAFSRS 82
Query: 409 NAFGVKEHNALLDALADPVKGWDLNNY 329
NAFGVKEHNALLDALADPVKGWDLNNY
Sbjct: 83 NAFGVKEHNALLDALADPVKGWDLNNY 109
>gb|PKK61813.1| hypothetical protein RhiirC2_791263 [Rhizophagus irregularis]
Length = 162
Score = 144 bits (362), Expect = 3e-36
Identities = 85/165 (51%), Positives = 101/165 (61%), Gaps = 24/165 (14%)
Frame = -2
Query: 769 MEQTLVIDSDISAGDKPFEVHEFLEALGLLDKEAS------------------PDF---- 656
ME+ L+IDS +S+ D +E+ L+ALGL+D E + PD
Sbjct: 1 MERNLLIDSGLSSVDGDYEL---LKALGLIDDEETEIVDGYDKIEADEEVLLGPDDDDDW 57
Query: 655 -RYEIXXXXXXXXXXXXXDN-YWDPRKRYTGWDYKRRFGEDALKKLYQTREARHRNIVLM 482
+E N YWDP K YTGWDY FGEDA KLYQ R A +RNIV+
Sbjct: 58 DEFETDDEDDDDDDDDDDRNNYWDPSKMYTGWDYLAHFGEDAWNKLYQARLAHYRNIVIR 117
Query: 481 EFTRRLPDISDREKNRLFDAFSRSNAFGVKEHNALLDALADPVKG 347
EFTRRLP+IS++EKN LFDAFSRS+AFGV EHNALLDALADPVKG
Sbjct: 118 EFTRRLPNISEQEKNGLFDAFSRSDAFGVIEHNALLDALADPVKG 162
>gb|PKK68463.1| hypothetical protein RhiirC2_867249 [Rhizophagus irregularis]
Length = 120
Score = 124 bits (312), Expect = 6e-30
Identities = 61/80 (76%), Positives = 68/80 (85%)
Frame = -2
Query: 577 YTGWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRSNAFG 398
YTGWDY FGEDA KLYQ R A +RNIV+ EFTRRLP+IS++EKN LFDAFSRS+AFG
Sbjct: 2 YTGWDYLAHFGEDAWNKLYQARLAHYRNIVIREFTRRLPNISEQEKNGLFDAFSRSDAFG 61
Query: 397 VKEHNALLDALADPVKGWDL 338
V EHNALLDALADPVKG +L
Sbjct: 62 VIEHNALLDALADPVKGRNL 81
>gb|PKY59596.1| hypothetical protein RhiirA4_482466 [Rhizophagus irregularis]
Length = 139
Score = 116 bits (290), Expect = 1e-26
Identities = 55/81 (67%), Positives = 66/81 (81%)
Frame = -2
Query: 571 GWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRSNAFGVK 392
G DY +G DA KL Q REARHRNI+L EFT RLP+IS++ KN LFD F++S+AFGV+
Sbjct: 59 GHDYIACYGRDAWDKLIQAREARHRNIILREFTTRLPNISEQAKNGLFDIFTKSSAFGVR 118
Query: 391 EHNALLDALADPVKGWDLNNY 329
EHNALLDALADPVKGW+L +Y
Sbjct: 119 EHNALLDALADPVKGWNLQDY 139
>gb|PKK58547.1| hypothetical protein RhiirC2_871526 [Rhizophagus irregularis]
Length = 137
Score = 115 bits (288), Expect = 2e-26
Identities = 55/81 (67%), Positives = 65/81 (80%)
Frame = -2
Query: 571 GWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRSNAFGVK 392
G DY +G DA KL Q REARHRNI+L EFT RLP+IS++ KN LFD F+ S+AFGV+
Sbjct: 57 GHDYIACYGRDAWDKLIQAREARHRNIILREFTTRLPNISEQAKNGLFDIFTESSAFGVR 116
Query: 391 EHNALLDALADPVKGWDLNNY 329
EHNALLDALADPVKGW+L +Y
Sbjct: 117 EHNALLDALADPVKGWNLQDY 137
>gb|PKC05693.1| hypothetical protein RhiirA5_420589 [Rhizophagus irregularis]
Length = 126
Score = 103 bits (256), Expect = 3e-22
Identities = 52/74 (70%), Positives = 59/74 (79%)
Frame = -2
Query: 583 KRYTGWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRSNA 404
K Y GWDYK+ FGEDA +KLY+ REAR R I+L FT RL +ISD+ KN LF AFSRSN+
Sbjct: 53 KLYDGWDYKKEFGEDAWEKLYRDREARQRFILLRLFTMRLSNISDQAKNGLFIAFSRSNS 112
Query: 403 FGVKEHNALLDALA 362
FGV EHNALLDALA
Sbjct: 113 FGVLEHNALLDALA 126
>gb|PKC00700.1| hypothetical protein RhiirA5_382268 [Rhizophagus irregularis]
gb|PKC61805.1| hypothetical protein RhiirA1_466006 [Rhizophagus irregularis]
gb|PKY30257.1| hypothetical protein RhiirB3_392421 [Rhizophagus irregularis]
Length = 144
Score = 102 bits (253), Expect = 1e-21
Identities = 51/81 (62%), Positives = 61/81 (75%)
Frame = -2
Query: 571 GWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRSNAFGVK 392
G DY +G DA KL Q +EARHRNI+L FT+RLP+IS++ KN LFD F+ S+AFGV+
Sbjct: 59 GHDYIACYGRDAWDKLIQAQEARHRNILLRLFTQRLPNISEQAKNGLFDIFTESSAFGVR 118
Query: 391 EHNALLDALADPVKGWDLNNY 329
EHNALLDALADPV G L Y
Sbjct: 119 EHNALLDALADPVNGLSLFEY 139
>gb|PKK57333.1| hypothetical protein RhiirC2_858257 [Rhizophagus irregularis]
gb|PKK68682.1| hypothetical protein RhiirC2_851269 [Rhizophagus irregularis]
Length = 146
Score = 100 bits (249), Expect = 4e-21
Identities = 51/81 (62%), Positives = 60/81 (74%)
Frame = -2
Query: 571 GWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRSNAFGVK 392
G DY +G DA KL Q REARHRNI+L FT+RLP+++++ KN LFDAFSRS AFGV
Sbjct: 61 GHDYIACYGRDAWDKLIQAREARHRNILLRLFTQRLPNLTEKVKNGLFDAFSRSEAFGVL 120
Query: 391 EHNALLDALADPVKGWDLNNY 329
EHNALL+ALA PV G L Y
Sbjct: 121 EHNALLEALAHPVNGLSLFEY 141
>gb|PKB98266.1| hypothetical protein RhiirA5_465269 [Rhizophagus irregularis]
gb|PKC54529.1| hypothetical protein RhiirA1_506429 [Rhizophagus irregularis]
gb|PKY34403.1| hypothetical protein RhiirB3_454074 [Rhizophagus irregularis]
Length = 141
Score = 98.2 bits (243), Expect = 3e-20
Identities = 50/81 (61%), Positives = 59/81 (72%)
Frame = -2
Query: 571 GWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRSNAFGVK 392
G DY +G DA KL Q REARHRNI+L FT+RLP+++++ KN LFDAFSRS AF V
Sbjct: 56 GHDYIACYGRDAWDKLIQAREARHRNILLRLFTQRLPNLTEKVKNGLFDAFSRSEAFSVL 115
Query: 391 EHNALLDALADPVKGWDLNNY 329
EHNALL+ALA PV G L Y
Sbjct: 116 EHNALLEALAHPVNGLSLFEY 136
>gb|PKK58473.1| hypothetical protein RhiirC2_857774 [Rhizophagus irregularis]
Length = 146
Score = 97.1 bits (240), Expect = 7e-20
Identities = 49/81 (60%), Positives = 59/81 (72%)
Frame = -2
Query: 571 GWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRSNAFGVK 392
G DY +G DA KL Q REARHRNI+L FT+RLP+++++ KN LFDAFSRS AF V
Sbjct: 61 GHDYIACYGRDAWDKLIQAREARHRNILLRLFTQRLPNLTEKVKNGLFDAFSRSEAFSVL 120
Query: 391 EHNALLDALADPVKGWDLNNY 329
EHNALL+ALA P+ G L Y
Sbjct: 121 EHNALLEALAHPMNGLSLFEY 141
>gb|PKY54691.1| hypothetical protein RhiirA4_426951 [Rhizophagus irregularis]
Length = 186
Score = 94.7 bits (234), Expect = 1e-18
Identities = 48/81 (59%), Positives = 59/81 (72%)
Frame = -2
Query: 571 GWDYKRRFGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRSNAFGVK 392
G D+ +G DA KL+Q R ARHRNI+L ++RLP++S++EKN LF AFS S AFGV
Sbjct: 103 GHDFMACYGRDAWDKLFQDRLARHRNILLRLLSQRLPNLSEQEKNGLFHAFSHSEAFGVL 162
Query: 391 EHNALLDALADPVKGWDLNNY 329
EHNALLDALA PV+G L Y
Sbjct: 163 EHNALLDALAHPVEGLSLFKY 183
>gb|PKY40192.1| hypothetical protein RhiirA4_453508 [Rhizophagus irregularis]
Length = 381
Score = 66.2 bits (160), Expect(2) = 5e-18
Identities = 31/31 (100%), Positives = 31/31 (100%)
Frame = +1
Query: 184 NNERIAEPKPEIVIPNRIQKIKEFDLLYPQI 276
NNERIAEPKPEIVIPNRIQKIKEFDLLYPQI
Sbjct: 351 NNERIAEPKPEIVIPNRIQKIKEFDLLYPQI 381
Score = 55.8 bits (133), Expect(2) = 5e-18
Identities = 28/30 (93%), Positives = 29/30 (96%)
Frame = +3
Query: 78 MSSELELLKQRITELEAENTELRKENTEIP 167
MSSELELLKQRIT+LEAENTELRK NTEIP
Sbjct: 312 MSSELELLKQRITKLEAENTELRKVNTEIP 341
>gb|PKK59138.1| hypothetical protein RhiirC2_762944 [Rhizophagus irregularis]
Length = 70
Score = 62.4 bits (150), Expect(2) = 2e-17
Identities = 30/31 (96%), Positives = 30/31 (96%)
Frame = +1
Query: 184 NNERIAEPKPEIVIPNRIQKIKEFDLLYPQI 276
NNERIAE KPEIVIPNRIQKIKEFDLLYPQI
Sbjct: 40 NNERIAELKPEIVIPNRIQKIKEFDLLYPQI 70
Score = 58.2 bits (139), Expect(2) = 2e-17
Identities = 29/30 (96%), Positives = 29/30 (96%)
Frame = +3
Query: 78 MSSELELLKQRITELEAENTELRKENTEIP 167
MSSELELLKQRITELEAEN ELRKENTEIP
Sbjct: 1 MSSELELLKQRITELEAENVELRKENTEIP 30
>gb|EXX62634.1| hypothetical protein RirG_159960 [Rhizophagus irregularis DAOM
197198w]
Length = 80
Score = 87.8 bits (216), Expect = 2e-17
Identities = 44/74 (59%), Positives = 54/74 (72%)
Frame = -2
Query: 550 FGEDALKKLYQTREARHRNIVLMEFTRRLPDISDREKNRLFDAFSRSNAFGVKEHNALLD 371
+G DA KL+Q R RHRNI+L ++RLP++S++EKN F AFS S AFGV EHNALLD
Sbjct: 4 YGRDAWDKLFQDRLDRHRNILLRLLSQRLPNLSEQEKNGFFHAFSHSEAFGVLEHNALLD 63
Query: 370 ALADPVKGWDLNNY 329
ALA PV+G L Y
Sbjct: 64 ALAHPVEGLSLFKY 77