BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon25_contig00022750
         (2941 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020273972.1| isoamylase 2, chloroplastic [Asparagus offic...  1173   0.0  
ref|XP_008799133.1| PREDICTED: isoamylase 2, chloroplastic [Phoe...  1113   0.0  
ref|XP_010938750.1| PREDICTED: isoamylase 2, chloroplastic [Elae...  1073   0.0  
ref|XP_020700621.1| isoamylase 2, chloroplastic [Dendrobium cate...  1037   0.0  
gb|PKA66852.1| Isoamylase 2, chloroplastic [Apostasia shenzhenica]   1032   0.0  
ref|XP_020087102.1| isoamylase 2, chloroplastic [Ananas comosus]...  1018   0.0  
ref|XP_010278620.1| PREDICTED: isoamylase 2, chloroplastic [Nelu...  1016   0.0  
gb|OAY83378.1| Isoamylase 2, chloroplastic [Ananas comosus]          1007   0.0  
ref|XP_018683242.1| PREDICTED: isoamylase 2, chloroplastic-like ...   988   0.0  
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   965   0.0  
gb|PIA39269.1| hypothetical protein AQUCO_02600010v1 [Aquilegia ...   964   0.0  
ref|XP_020592870.1| isoamylase 2, chloroplastic [Phalaenopsis eq...   961   0.0  
ref|XP_011624242.1| isoamylase 2, chloroplastic isoform X2 [Ambo...   954   0.0  
gb|ERN08315.1| hypothetical protein AMTR_s00156p00067780 [Ambore...   954   0.0  
ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber] ...   953   0.0  
gb|AOQ26242.1| ISA2 [Actinidia deliciosa]                             944   0.0  
gb|OMO77794.1| hypothetical protein CCACVL1_14832 [Corchorus cap...   939   0.0  
ref|XP_018858427.1| PREDICTED: isoamylase 2, chloroplastic [Jugl...   938   0.0  
gb|OMP04997.1| hypothetical protein COLO4_09141 [Corchorus olito...   935   0.0  
ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]...   935   0.0  

>ref|XP_020273972.1| isoamylase 2, chloroplastic [Asparagus officinalis]
 ref|XP_020273973.1| isoamylase 2, chloroplastic [Asparagus officinalis]
 gb|ONK62840.1| uncharacterized protein A4U43_C07F8670 [Asparagus officinalis]
          Length = 855

 Score = 1173 bits (3035), Expect = 0.0
 Identities = 590/871 (67%), Positives = 670/871 (76%), Gaps = 9/871 (1%)
 Frame = -2

Query: 2700 KSGIIKRVLVPWCFFRKNIVSAKQVHPLSNCRPGRKFPEDATIDAHKLRNVKISEASHNS 2521
            K+ I     +PW     ++   KQ  PL  C+ GR         AHK +N K+    HNS
Sbjct: 13   KTYIQASTFIPW----GSVPFCKQTVPLKICKSGR------LNYAHKPQNAKVFLKPHNS 62

Query: 2520 TEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEH 2341
             EQI PG  LNYNAE  QR  SYLFRTE+GGLVKVLVG NNMKY V I+VSSLP   N  
Sbjct: 63   IEQIHPGSDLNYNAETGQRATSYLFRTEIGGLVKVLVGFNNMKYAVRIDVSSLPTRTN-- 120

Query: 2340 DLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDS 2161
            DLILSWG+ RSDS+ L AP                     +TP  QN  G  ++TLEF S
Sbjct: 121  DLILSWGVHRSDSNQLDAP------------------GCTKTPLVQNLKGRFSVTLEFSS 162

Query: 2160 AQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSEDGLVNFAL 1981
             Q+P                +SGI+THR TNFC+PVG+S+G PMPLG S+ E GLVNFAL
Sbjct: 163  TQIPFYLSFLLHSSSDHPRCESGIRTHRMTNFCLPVGMSAGHPMPLGVSRCEGGLVNFAL 222

Query: 1980 FSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITW 1801
            FSR AESV L L+++  N+P L++ELDPY+NRTGDIWH+SME+VG+Y SYGYRCKGE   
Sbjct: 223  FSRNAESVVLRLFDNNVNEPCLELELDPYVNRTGDIWHLSMENVGSYTSYGYRCKGETGS 282

Query: 1800 DNGSRFHASHVLLDPYAKLIKNFIPDQGES----VYLGYLGDEPTFDWSGDVHPQLPMEK 1633
            D GSRFHA  VLLDPYAKLI N  P++GES      LG L +EP FDWSGDVHPQLPMEK
Sbjct: 283  DKGSRFHARQVLLDPYAKLIANVSPEKGESRSLVKCLGSLAEEPIFDWSGDVHPQLPMEK 342

Query: 1632 LVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFPYDEKRGPY 1453
            LVVYRLNVG+FTKD+SSELPENVAGTFAGVIEKL H KSLG+NA+LLEPIF +DEK GPY
Sbjct: 343  LVVYRLNVGNFTKDESSELPENVAGTFAGVIEKLPHLKSLGINAILLEPIFTFDEKEGPY 402

Query: 1452 FPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEGG-TACQTIS 1276
            FPYHFFSPMNSYG   D +ST+ SMK+MVK LHAHGIEVLLEVVFTHTGEGG ++ QT S
Sbjct: 403  FPYHFFSPMNSYGYKSDSISTINSMKDMVKTLHAHGIEVLLEVVFTHTGEGGDSSSQTKS 462

Query: 1275 FRGIDNSSYYITEGGMGSGM----NCNNPIVQQLILDSLHYWLTEFHVDGFCFINSSYLL 1108
            FRGID SSYYI EG M  G+    N N+P+VQQLIL+SLHYWLTEFH+DGFCFINSSYLL
Sbjct: 463  FRGIDYSSYYIVEGDMTQGIYNAVNSNDPVVQQLILESLHYWLTEFHIDGFCFINSSYLL 522

Query: 1107 RGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDIHFPHWRRWAEINARFCR 928
            RGSNG+ LS PPLVEAI+FDPLLS++KIIADCW PVD S+ DIHFPHW+RWAE+N RFCR
Sbjct: 523  RGSNGEYLSHPPLVEAISFDPLLSKSKIIADCWSPVDGSYLDIHFPHWKRWAEMNTRFCR 582

Query: 927  DVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVSFSSNELAS 748
            DVRNFLRGEGLLSDLATRLCGSGDLFSDSRGP FSFNYVTKNFGLPLVDLVSFS +ELAS
Sbjct: 583  DVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPAFSFNYVTKNFGLPLVDLVSFSRDELAS 642

Query: 747  ELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTGGLFAYDGR 568
            ELSWNCG+EG TN   VLE+RL+QIRNFLF+LFISLG+PV NMGDECGFSTGGL +Y  R
Sbjct: 643  ELSWNCGKEGPTNNNSVLEIRLRQIRNFLFLLFISLGVPVLNMGDECGFSTGGLSSYGDR 702

Query: 567  QPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQSDPKWEDP 388
             PIDWN  K+V+GKQITQF+ FLSSLR RR+D+FQ RDFLKV+NI W GSN S PKWEDP
Sbjct: 703  HPIDWNVTKSVFGKQITQFITFLSSLRNRRNDIFQRRDFLKVENIVWQGSNLSQPKWEDP 762

Query: 387  SCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCLVDTALPYP 208
            SCKFLA+T+RA+K+  + +S KGDLF+CFN  D PE  ILP L EGY W  LVDT+LP+P
Sbjct: 763  SCKFLAVTIRADKDDNMSTSYKGDLFICFNADDFPEIAILPPLLEGYEWFRLVDTSLPFP 822

Query: 207  GFFSMDSNFCDGLSTYELKPHSCTLFEAGMS 115
            GFFS +S F + L  YELKPHSC LFE   S
Sbjct: 823  GFFSGESVFTEALLGYELKPHSCVLFEVKKS 853


>ref|XP_008799133.1| PREDICTED: isoamylase 2, chloroplastic [Phoenix dactylifera]
          Length = 853

 Score = 1113 bits (2880), Expect = 0.0
 Identities = 551/847 (65%), Positives = 660/847 (77%), Gaps = 15/847 (1%)
 Frame = -2

Query: 2610 CRPGRKFPED--ATIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTE 2437
            CR   K P    ++  +H+L    +   S +S EQ+ P LG++ + ++LQ+ FSY+FRTE
Sbjct: 32   CRYSGKVPMSLHSSRSSHELI---VCAMSQSSVEQLAPDLGVSDSTQELQKPFSYMFRTE 88

Query: 2436 LGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDT 2257
            +GGLVKVLVG  +M+Y V +EVSSLP  + E  L+++WGMFRSDSS L A DSQ +    
Sbjct: 89   IGGLVKVLVGTKSMRYAVRVEVSSLPRAVGEDGLVMNWGMFRSDSSQLLAQDSQVA---- 144

Query: 2256 SQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHR 2077
                           F +NSLG + + LEFDSA++P                 S I+THR
Sbjct: 145  ---------------FVRNSLGGYMVELEFDSARIPFYLSFLLSSPPSV----SEIRTHR 185

Query: 2076 KTNFCVPVGISSGRPMPLGFSKSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDP 1897
            KT FCVPVG+  G PMPLG S S+DG VNF+LFSR AE V LCL++    +PSL+++LDP
Sbjct: 186  KTKFCVPVGLGPGWPMPLGVSISDDGAVNFSLFSRNAEGVVLCLFDGKTREPSLEMDLDP 245

Query: 1896 YINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQG 1717
            Y+NRTGDIWHVSMES+GN+VSYGYRCKG   WD   RFHA HVLLDPYAK++ +F+PD+G
Sbjct: 246  YVNRTGDIWHVSMESMGNFVSYGYRCKGSGLWDKEDRFHAHHVLLDPYAKVLGDFVPDRG 305

Query: 1716 ESVYL----GYLGDEPTFDWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFA 1549
            ESV L    G LG E +FDWSGDVHP+LPMEKLVVYRLNVG FTK+K+S LPENV+GTFA
Sbjct: 306  ESVSLAKCLGSLGFEHSFDWSGDVHPRLPMEKLVVYRLNVGQFTKNKTSGLPENVSGTFA 365

Query: 1548 GVIEKLQHFKSLGVNAVLLEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEM 1369
            G+IEK++HF++LGVNAVLLEPIF +DE +GPYFPYHFFSPM+SYG   DG+S + SMKEM
Sbjct: 366  GLIEKVEHFRTLGVNAVLLEPIFSFDEHKGPYFPYHFFSPMHSYGHASDGVSAINSMKEM 425

Query: 1368 VKALHAHGIEVLLEVVFTHTGE-GGTACQTISFRGIDNSSYYITEGGMGSG----MNCNN 1204
            VK LHAHGIEVLLEV FT TGE G  ACQTISFRGIDNSSYYI +G +GSG    + C+N
Sbjct: 426  VKMLHAHGIEVLLEVAFTQTGEEGDAACQTISFRGIDNSSYYIVDGDLGSGAYNVLKCSN 485

Query: 1203 PIVQQLILDSLHYWLTEFHVDGFCFINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKI 1024
            P+VQQLILDSL +W+ EFHVDGFCFINSS+L RG NGD LS PPLVEAIA DP+LS+TKI
Sbjct: 486  PVVQQLILDSLRHWVVEFHVDGFCFINSSFLARGLNGDYLSRPPLVEAIAVDPILSKTKI 545

Query: 1023 IADCWCPVDMSHSDIHFPHWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSD 844
            IADCW P+D S+ +I FPHW+RWAE+N RFC DVRNFLRGEGLL DLATRLCGSGDLFS 
Sbjct: 546  IADCWSPLDNSYMEIQFPHWKRWAEMNTRFCSDVRNFLRGEGLLRDLATRLCGSGDLFS- 604

Query: 843  SRGPTFSFNYVTKNFGLPLVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNF 664
            SRGP FSFNY+TKNFGL LVDLVSFS+ +LASELSWNCGEEG+TN   VL++RLKQIRNF
Sbjct: 605  SRGPAFSFNYITKNFGLTLVDLVSFSNGDLASELSWNCGEEGSTNNNTVLDIRLKQIRNF 664

Query: 663  LFILFISLGIPVFNMGDECGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRI 484
            LF+LF+SLG+PV NMGDECG+STGG  +Y GRQPI WNGL+TV+G+QITQF+AF+SSLR+
Sbjct: 665  LFVLFVSLGVPVLNMGDECGYSTGGSPSYIGRQPIGWNGLQTVFGRQITQFIAFMSSLRM 724

Query: 483  RRSDLFQMRDFLKVKNIDWHGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVC 304
            RR D+FQ R+FL+++NIDWHGS+QS P W  PSCKFLAMTLRAE + KL +SD GDLF+ 
Sbjct: 725  RRGDIFQRRNFLELENIDWHGSSQSRPNWGKPSCKFLAMTLRAEMDDKLSNSDDGDLFIS 784

Query: 303  FNGSDLPESVILPQLPEGYAWVCLVDTALPYPGFFSMDS----NFCDGLSTYELKPHSCT 136
            FN S  PES +LP+  EG  W+ LVDT+LP+PGFFS DS    +   GLS+YELKPHSC 
Sbjct: 785  FNASAHPESTVLPEQSEGVVWLRLVDTSLPFPGFFSSDSDPSVHQAAGLSSYELKPHSCV 844

Query: 135  LFEAGMS 115
            LFEA  S
Sbjct: 845  LFEAKRS 851


>ref|XP_010938750.1| PREDICTED: isoamylase 2, chloroplastic [Elaeis guineensis]
          Length = 851

 Score = 1073 bits (2775), Expect = 0.0
 Identities = 528/823 (64%), Positives = 631/823 (76%), Gaps = 13/823 (1%)
 Frame = -2

Query: 2544 ISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSS 2365
            +S++S    EQ+ P L ++ + ++L++ FSY+FR E GGL KVLVG  +MKY V +EVS 
Sbjct: 52   MSQSSVEHVEQLAPDLEVSDSTQELEKPFSYIFRAETGGLAKVLVGVKDMKYAVRVEVSF 111

Query: 2364 LPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTH 2185
            LP  + E  L+++WGMFRSDS  L   DSQ +                   F QNSLG +
Sbjct: 112  LPRAVGEDGLVMNWGMFRSDSKQLLVQDSQVA-------------------FVQNSLGGY 152

Query: 2184 AITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSE 2005
             + LEFDS ++P                 S I+TH+KT FCVPVGI  G PMPLG S S+
Sbjct: 153  MVELEFDSVEIPFYLSFLLSSPSV-----SEIRTHKKTKFCVPVGIGPGCPMPLGVSISD 207

Query: 2004 DGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGY 1825
            DG +NF++FSR AE V L L++    +PSL+I+LDPY+NRTGDIWHVSMES+ NY  YGY
Sbjct: 208  DGAINFSVFSRNAEGVVLYLFDGRTREPSLEIDLDPYVNRTGDIWHVSMESIENYAGYGY 267

Query: 1824 RCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVYL----GYLGDEPTFDWSGDV 1657
            RCKG   WD G +FHA  +LLDPYAK++ +F+ DQGES+ L    G L  E  FDW+GDV
Sbjct: 268  RCKGLALWDKGDKFHAHDILLDPYAKVLGDFVLDQGESMSLAKCLGSLDIEHGFDWTGDV 327

Query: 1656 HPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFP 1477
            HP LPMEKLVVYRLNVG FTK K+S LPENVAGTFAG+IEK++H KSLGVNAVLLEPIF 
Sbjct: 328  HPCLPMEKLVVYRLNVGQFTKHKTSRLPENVAGTFAGLIEKVEHLKSLGVNAVLLEPIFS 387

Query: 1476 YDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEG- 1300
            +DE++GPYFPYHFFSPMNSYG   DG+S + SMK+MVK LHAHGIEVLLEV FT TGEG 
Sbjct: 388  FDEQKGPYFPYHFFSPMNSYGYASDGVSAIDSMKKMVKMLHAHGIEVLLEVAFTQTGEGE 447

Query: 1299 GTACQTISFRGIDNSSYYITEGGMGSG----MNCNNPIVQQLILDSLHYWLTEFHVDGFC 1132
              AC+TISFRGIDNSSYYI +  +G G    + C+NP+VQQLILDSL +W+ EFHVDGFC
Sbjct: 448  DAACRTISFRGIDNSSYYIVDRDLGYGAHNALKCSNPVVQQLILDSLRHWVIEFHVDGFC 507

Query: 1131 FINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDIHFPHWRRWA 952
            FINSS+L +G NGD L  PPLVEAIAFDP+LS+TKIIADCW P D S+ +I FPHW+RWA
Sbjct: 508  FINSSFLAQGLNGDYLYRPPLVEAIAFDPILSKTKIIADCWSPPDNSYMEIRFPHWKRWA 567

Query: 951  EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 772
            E+N RFC DVRNFLRGEGLLS+LATRLCGSGDLFS SRGP FSFNY+TKNFGL LVDLVS
Sbjct: 568  EMNTRFCSDVRNFLRGEGLLSNLATRLCGSGDLFS-SRGPAFSFNYITKNFGLSLVDLVS 626

Query: 771  FSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTG 592
            +S  +LASELSWNCGEEG TN   VL++RLKQIRNFLF+LF+SLG+PV NMGDECG+STG
Sbjct: 627  YSDGDLASELSWNCGEEGPTNNNTVLDLRLKQIRNFLFVLFVSLGVPVLNMGDECGYSTG 686

Query: 591  GLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQ 412
            G  +Y  R+PI WNGLKTV+G+QITQF+AFLSSLR+RR D+FQ RDFLKV+NIDWHGS+Q
Sbjct: 687  GTPSYIDRKPIGWNGLKTVFGRQITQFIAFLSSLRVRRGDIFQRRDFLKVENIDWHGSSQ 746

Query: 411  SDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCL 232
            S P W +PSCKFLA+TL+AE + KL +S+ GDLF+ FN SDL ES +LP+  EG  W+ L
Sbjct: 747  SQPNWREPSCKFLAVTLKAEMDDKLSNSNDGDLFISFNASDLLESAVLPEQSEGIVWLRL 806

Query: 231  VDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEAGMS 115
            VDTALP+PGFFS DS    +   GLS+Y+LKPHSC LFEA  S
Sbjct: 807  VDTALPFPGFFSSDSDPDVHQAAGLSSYDLKPHSCVLFEAKRS 849


>ref|XP_020700621.1| isoamylase 2, chloroplastic [Dendrobium catenatum]
 gb|PKU77045.1| Isoamylase 2, chloroplastic [Dendrobium catenatum]
          Length = 894

 Score = 1037 bits (2681), Expect = 0.0
 Identities = 526/856 (61%), Positives = 631/856 (73%), Gaps = 14/856 (1%)
 Frame = -2

Query: 2649 NIVSAKQVHPLSNCRPGRKFPEDATIDAHKLRNVKISEASHNST-EQIQPGLGLNYNAEK 2473
            NI   + V+    CR  RKF  DA+ D+ + R  ++      ST E+ QP L +  + EK
Sbjct: 37   NIPLTRPVYAERRCRSCRKFLCDASNDSLQNRTSEMFTGVARSTKEEAQPVLEMTSDFEK 96

Query: 2472 LQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHL 2293
              + +S LFRTE  GLVKV VGA  MKY V IEV SLP  L  H+LIL WGMFRSDS   
Sbjct: 97   QNKGYSLLFRTEFDGLVKVFVGAIKMKYTVCIEVQSLPQSLTSHNLILFWGMFRSDSLPF 156

Query: 2292 YAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXX 2113
            + PDS +S+L T++   +  +  ++TPF ++S   H + L+FD   VP            
Sbjct: 157  HGPDSHSSVLPTTKFKPEASQHLMKTPFVRDSC-RHILVLDFDQNDVPFYLSFFLQSDGT 215

Query: 2112 XLATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSEDGLVNFALFSRKAESVNLCLYESG 1933
                +  I+THRKTNFCVPVG+  G PM LG S S DGL NF+LFSR A+SV LC Y+S 
Sbjct: 216  --GQERTIRTHRKTNFCVPVGLGPGYPMALGVSSSNDGLANFSLFSRNAKSVVLCFYKSK 273

Query: 1932 ANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPY 1753
             ++PSL+I+LDPY+N TGDIWHVS+ S+ NY+ YGYRCKG+I+WD GS  +A HVLLDPY
Sbjct: 274  TSEPSLEIDLDPYVNHTGDIWHVSVHSITNYIGYGYRCKGDISWDGGSSSNAHHVLLDPY 333

Query: 1752 AKLIKNFIPDQGESVY----LGYLGDEPTFDWSGDVHPQLPMEKLVVYRLNVGSFTKDKS 1585
            AK I N   DQGES      LGYL  EP FDW+GD HPQ+PMEKL++YRLNV  FTK +S
Sbjct: 334  AKSIGNLSSDQGESACMVKCLGYLRKEPNFDWNGDTHPQIPMEKLIIYRLNVRQFTKSES 393

Query: 1584 SELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFPYDEKRGPYFPYHFFSPMNSYGCTR 1405
            SEL  NVAGTFAGV++K+QH  +LG+NAVLLEP+F +D+KRGPYFPYHFFSPM+ YG   
Sbjct: 394  SELAINVAGTFAGVVKKVQHLINLGINAVLLEPVFSFDKKRGPYFPYHFFSPMSIYGGVD 453

Query: 1404 DGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGE-GGTACQTISFRGIDNSSYYITEGGM 1228
            + +S+V SMKEMVK LH+HGIEVLLEVVFTHT E G +  Q IS  GIDN SYYI +G +
Sbjct: 454  ESISSVYSMKEMVKILHSHGIEVLLEVVFTHTCEVGDSDSQIISLLGIDNPSYYIVDGNV 513

Query: 1227 G----SGMNCNNPIVQQLILDSLHYWLTEFHVDGFCFINSSYLLRGSNGDRLSPPPLVEA 1060
                 S + CN+PIVQQ+ILDSLH+W+TEFHVDGFCFINSS LL+GSNGD L  PPLVE 
Sbjct: 514  HLENCSALKCNDPIVQQMILDSLHHWVTEFHVDGFCFINSSSLLQGSNGDHLPRPPLVED 573

Query: 1059 IAFDPLLSETKIIADCWCPVDMSHSDIHFPHWRRWAEINARFCRDVRNFLRGEGLLSDLA 880
            IAFDPLLSETKII+DCW P+DMS+    FPHW+RWAE+N+++  DVRNFLRG+GLLS LA
Sbjct: 574  IAFDPLLSETKIISDCWSPIDMSYKYAIFPHWQRWAEMNSKYFLDVRNFLRGQGLLSHLA 633

Query: 879  TRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVSFSSNELASELSWNCGEEGATNKKL 700
            TRLCGSGD+F+ SRGP +SFNY+TKN GL LVDLVSFS  +LASELSWNCG EG T K  
Sbjct: 634  TRLCGSGDIFTASRGPAYSFNYITKNAGLTLVDLVSFSGADLASELSWNCGVEGPTEKNS 693

Query: 699  VLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTGGLFAYDGRQPIDWNGLKTVYGKQI 520
            VLE+RL+Q+RNFLFILFISLG+PV NMGDECG+STGGL  YD R PIDW  L+T +  QI
Sbjct: 694  VLEIRLRQVRNFLFILFISLGVPVINMGDECGYSTGGLPFYDNRVPIDWKSLRTGFSIQI 753

Query: 519  TQFVAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQSDPKWEDPSCKFLAMTLRAEKNGK 340
            T+FVAFL+SLR RRSD+FQ + F K +NI W+GSN S PKWEDPSCKFLAM L+AEK+ K
Sbjct: 754  TKFVAFLASLRNRRSDVFQRKIFYKAENITWYGSNLSLPKWEDPSCKFLAMELKAEKDVK 813

Query: 339  LPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCLVDTALPYPGFFSMDSNFCD----G 172
              +S  GDLF+  N SDLPE V LP+   GY W  LVDT+LPYP FFS DSN       G
Sbjct: 814  SRNSYNGDLFITINASDLPELVTLPESSAGYVWFRLVDTSLPYPYFFSSDSNPNSQKNAG 873

Query: 171  LSTYELKPHSCTLFEA 124
            LS+YELKPH+C LFEA
Sbjct: 874  LSSYELKPHTCALFEA 889


>gb|PKA66852.1| Isoamylase 2, chloroplastic [Apostasia shenzhenica]
          Length = 881

 Score = 1032 bits (2668), Expect = 0.0
 Identities = 522/857 (60%), Positives = 624/857 (72%), Gaps = 13/857 (1%)
 Frame = -2

Query: 2655 RKNIVSAKQVHPLSNCRPGRKFPEDATIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAE 2476
            + NI+  +QV+     R G  FP+ +  +A +    ++  A+ +S + +QP   + +N E
Sbjct: 35   KSNILLMQQVNAHRRHRTGESFPQGSLKNAVRKPTSRVLAAAPSSQKDVQPVSDVTFNFE 94

Query: 2475 KLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSH 2296
              Q+ +SYLFRTE  G+VKVLV  N+MKY V IE+ SLP  + +HDLIL W  FRSDS +
Sbjct: 95   DQQKEYSYLFRTEFDGMVKVLVRINDMKYTVYIELQSLPKPIIDHDLILCWHTFRSDSEN 154

Query: 2295 LYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXX 2116
                 SQ S L             ++TP T+ S G H   LEF   Q+P           
Sbjct: 155  SSGLGSQISALSADHF--------MKTPLTRGSSGMHTAVLEFSFQQIPFYLSFLLQVDG 206

Query: 2115 XXLATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSEDGLVNFALFSRKAESVNLCLYES 1936
               A  S IKT RKT F VPVG+ SG  +PLG S  +DG  NF+LFSR AESV LCLY+ 
Sbjct: 207  ---AAKSEIKTERKTYFRVPVGLGSGHSLPLGISLFDDGSANFSLFSRNAESVVLCLYDG 263

Query: 1935 GANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDP 1756
              N+PS +IELDPY+NRTGDIWHV +  + NY  YGYRCKG+I WD GSRFHA HVLLDP
Sbjct: 264  KTNEPSFEIELDPYVNRTGDIWHVLLYDLSNYTRYGYRCKGKIFWDTGSRFHARHVLLDP 323

Query: 1755 YAKLIKNFIPDQGESVYL----GYLGDEPTFDWSGDVHPQLPMEKLVVYRLNVGSFTKDK 1588
            YAK I  F PD+G SV L    G L   P FDW+GD HPQ+ MEKLVVYRLNVG FTK+K
Sbjct: 324  YAKTIGEFCPDEGGSVSLSKCLGCLRKMPKFDWNGDTHPQISMEKLVVYRLNVGRFTKEK 383

Query: 1587 SSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFPYDEKRGPYFPYHFFSPMNSYGCT 1408
            SS LPENVAGTFAGV +K+QH  SLGVNAVLLEPIFP+D+ R PYFPYHFFSPMN YG  
Sbjct: 384  SSGLPENVAGTFAGVTKKIQHLTSLGVNAVLLEPIFPFDKNRNPYFPYHFFSPMNEYGDV 443

Query: 1407 RDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEGG-TACQTISFRGIDNSSYYITE-- 1237
             +  S V  MKEMVK LH +GIEVL+EVVFTHT EG  T  + ISFRGIDNSSYYI E  
Sbjct: 444  DEFNSAVNLMKEMVKTLHTNGIEVLMEVVFTHTCEGNDTKSRIISFRGIDNSSYYIVEPN 503

Query: 1236 --GGMGSGMNCNNPIVQQLILDSLHYWLTEFHVDGFCFINSSYLLRGSNGDRLSPPPLVE 1063
               G+ S +NCN+  VQQ+ILDSLHYWLTEFHVDGFCFINSS LL+GSN D L   PL+E
Sbjct: 504  GASGINSMLNCNSAAVQQIILDSLHYWLTEFHVDGFCFINSSSLLQGSNSDDLPRSPLIE 563

Query: 1062 AIAFDPLLSETKIIADCWCPVDMSHSDIHFPHWRRWAEINARFCRDVRNFLRGEGLLSDL 883
            AIAFDPLL++TKII+DCW P+D S  D+ FPHWRRW E+N +F RDVRNFL+GEGLLS+ 
Sbjct: 564  AIAFDPLLTKTKIISDCWSPIDESCKDVVFPHWRRWGEMNPKFSRDVRNFLKGEGLLSEF 623

Query: 882  ATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVSFSSNELASELSWNCGEEGATNKK 703
            ATRLCGSGD+F  SRGP++SFNYVTKNFGL LVDLVSFS  +LASELSWNCG+EG T+K 
Sbjct: 624  ATRLCGSGDIFLHSRGPSYSFNYVTKNFGLTLVDLVSFSGADLASELSWNCGDEGPTDKS 683

Query: 702  LVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTGGLFAYDGRQPIDWNGLKTVYGKQ 523
            +VLE+RL+QIRNFLFILFISLG+PV NMGDECG+S GG  +YD R P+DW+ L+T +G+Q
Sbjct: 684  IVLEIRLRQIRNFLFILFISLGVPVLNMGDECGYSAGGSPSYDERMPLDWSNLRTEFGRQ 743

Query: 522  ITQFVAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQSDPKWEDPSCKFLAMTLRAEKNG 343
            IT F++FL+S R RRSD+FQ ++FL+++NI WHGSNQS  KWEDPSCKFLAM L+AE++ 
Sbjct: 744  ITSFISFLTSFRDRRSDVFQRKEFLELENIAWHGSNQSPLKWEDPSCKFLAMDLKAEEDA 803

Query: 342  KLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCLVDTALPYPGFFS--MDSNFC--D 175
                S  G+LF+ FN S   ES  LP LPEG AW  LVDT+LPYPGFFS   D++ C  +
Sbjct: 804  GSSDSGNGNLFISFNASADIESANLPGLPEGQAWFRLVDTSLPYPGFFSSDFDADVCKFE 863

Query: 174  GLSTYELKPHSCTLFEA 124
            GLS+YELKPHSC LFEA
Sbjct: 864  GLSSYELKPHSCALFEA 880


>ref|XP_020087102.1| isoamylase 2, chloroplastic [Ananas comosus]
 ref|XP_020087103.1| isoamylase 2, chloroplastic [Ananas comosus]
          Length = 863

 Score = 1018 bits (2632), Expect = 0.0
 Identities = 510/825 (61%), Positives = 619/825 (75%), Gaps = 14/825 (1%)
 Frame = -2

Query: 2541 SEASHNSTEQIQPGLGLNYNA-EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSS 2365
            S AS +   ++ P +G+   + E+LQ+ FSY FRT+  G+VKV+V +   KYVV  EV+ 
Sbjct: 55   SSASRSPIRRLHPSVGVKIGSKEELQKGFSYTFRTDSNGIVKVVVRSEYGKYVVRFEVTF 114

Query: 2364 LPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTH 2185
            LP +  E  L+L+W MF SDSS L  P SQ S           G  + + PF QNS G+ 
Sbjct: 115  LPQFGTEEGLVLNWTMFTSDSSLLLVPCSQASE----------GTVTAKVPFVQNSSGSF 164

Query: 2184 AITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSE 2005
               L FD+++VP              +  S I++HRKTNFCVPVG+ SG+PMPLG S S+
Sbjct: 165  LAELVFDASKVPFYLSFLLN------SAGSEIRSHRKTNFCVPVGLGSGQPMPLGVSVSD 218

Query: 2004 DGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGY 1825
            DGL NF+LFS+ AE V LCLY+   ++P+L+IELDPYIN TGDIWHVS+E+V  YVSYGY
Sbjct: 219  DGLTNFSLFSKNAEGVILCLYDGIKDEPALEIELDPYINCTGDIWHVSLENVEEYVSYGY 278

Query: 1824 RCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVYLGY----LGDEPTFDWSGDV 1657
            RCKG I W  G RFH  HVLLDPYAK+I NF PD G  V L      L  E  FDWSGD 
Sbjct: 279  RCKGPILWRKGDRFHMKHVLLDPYAKVIGNFFPDHGGKVSLSRCLASLAKESAFDWSGDT 338

Query: 1656 HPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFP 1477
             P LPMEKLVVYRLNVG  TKDKSS L EN AGTF+G+IEK+++FK+LGVNAVLLEPIFP
Sbjct: 339  SPCLPMEKLVVYRLNVGLCTKDKSSGLSENAAGTFSGLIEKIEYFKTLGVNAVLLEPIFP 398

Query: 1476 YDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEGG 1297
            +DEK+GPYFP+HFFSPM SYG  RD  S   SMKEM+K++HA G+EVLLEVVF+HT EGG
Sbjct: 399  FDEKKGPYFPFHFFSPMMSYGHGRDSASGTNSMKEMIKSMHARGMEVLLEVVFSHTSEGG 458

Query: 1296 TAC-QTISFRGIDNSSYYITEGGMGSG----MNCNNPIVQQLILDSLHYWLTEFHVDGFC 1132
             A  Q ISFRGIDNSSYYI +G + SG    +NCNNPIVQ+LILDSLH+W+ E+HVDGFC
Sbjct: 459  DAASQMISFRGIDNSSYYIVDGDVRSGANNALNCNNPIVQRLILDSLHHWVVEYHVDGFC 518

Query: 1131 FINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDIHFPHWRRWA 952
            F+NSS L+R SNG+ LS PPL+EAIAFDP+LS+TKIIADCW P+DMS  +I FPHW++WA
Sbjct: 519  FVNSSSLVRSSNGNNLSRPPLLEAIAFDPILSKTKIIADCWSPIDMSSMEIQFPHWKKWA 578

Query: 951  EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 772
            E+N+RF  DVRNFLRGE LLSDLATR+CGSGDLFS SRGP +SFNY+TKNFGLPLVDLVS
Sbjct: 579  EMNSRFSVDVRNFLRGEALLSDLATRICGSGDLFS-SRGPAYSFNYITKNFGLPLVDLVS 637

Query: 771  FSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTG 592
            FSS +LASELSWNCGEEG T    VL+ RLKQIRNFLFILF+SLG+PV NMGDECG+S G
Sbjct: 638  FSSIDLASELSWNCGEEGPTGNSSVLQTRLKQIRNFLFILFVSLGVPVLNMGDECGYSNG 697

Query: 591  GLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQ 412
            G  +Y+ R+P DWN L+T +G QITQFVA+LSSLR RR D+FQ +DFLKV+NI W+GSNQ
Sbjct: 698  GSPSYNDRRPFDWNSLRTGFGLQITQFVAYLSSLRNRRGDIFQRKDFLKVENICWNGSNQ 757

Query: 411  SDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCL 232
            ++P W D SCKFL++  +A+ +G+     +GDLF+ FN SD PE  +LP+  EG  W+ L
Sbjct: 758  TEPNWGDSSCKFLSVMFKADVDGRASKLHRGDLFIGFNASDHPEVALLPEQSEGTVWLRL 817

Query: 231  VDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEAGMSDL 109
            VDTALP+PGFF+  S    +   GLS YE+K HSC+LFEA  ++L
Sbjct: 818  VDTALPFPGFFASYSDPNVHQVAGLSAYEIKSHSCSLFEAKRTEL 862


>ref|XP_010278620.1| PREDICTED: isoamylase 2, chloroplastic [Nelumbo nucifera]
 ref|XP_010278628.1| PREDICTED: isoamylase 2, chloroplastic [Nelumbo nucifera]
          Length = 890

 Score = 1016 bits (2627), Expect = 0.0
 Identities = 511/837 (61%), Positives = 616/837 (73%), Gaps = 17/837 (2%)
 Frame = -2

Query: 2583 DATIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGA 2404
            D T +A    N+KI  +S +S +Q +P LG     E++++  +YLFRTE+GG VK+ VG 
Sbjct: 56   DITKNAQWYHNLKIFASSPSSVDQTRPSLGTFTKTEEVEKPLTYLFRTEIGGQVKITVGR 115

Query: 2403 NNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRAS 2224
             N  Y V IEVSSLP    E  L L+WG+FRSDSS L  P SQ S  +T  ST +     
Sbjct: 116  KNTNYTVCIEVSSLPQCNTEDKLFLNWGIFRSDSSCLTLPGSQVSAPETHSSTME----- 170

Query: 2223 IRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGIS 2044
              TP  Q S G H + LEF+S Q P                +S I++HRKTNFCVPVGIS
Sbjct: 171  --TPLMQKSSGRHFLQLEFESNQAPFYLSFMLLFSSNTATNNSEIRSHRKTNFCVPVGIS 228

Query: 2043 SGRPMPLGFSKSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHV 1864
            SG P PLG S S+DG VNF+LFSR AESV LCLY+  ++QPSL+I+LDPYINRTG+IWHV
Sbjct: 229  SGHPAPLGISFSDDGSVNFSLFSRNAESVVLCLYDEKSDQPSLEIDLDPYINRTGNIWHV 288

Query: 1863 SMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGES----VYLGY 1696
            SMESV  YVSYGYRCKG+I    G RF A +VLLDPYAK++ + +P+  E+      LG+
Sbjct: 289  SMESVAPYVSYGYRCKGDIHLKKGDRFDARNVLLDPYAKILSSSLPNHSETHSPPKRLGH 348

Query: 1695 LGDEPTFDWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKS 1516
            L   PTFDWSGD+ P L +E+LVVYRLNV  FT+DKS +LP +V GTF+G+IEKL HFKS
Sbjct: 349  LCKIPTFDWSGDIRPCLEIEELVVYRLNVRRFTEDKSCQLPADVLGTFSGLIEKLHHFKS 408

Query: 1515 LGVNAVLLEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEV 1336
            LGVNAVLLEP+F + E+ GPYFPYHFFS  + YG + D +ST+ SMKEM+K LHA+GIEV
Sbjct: 409  LGVNAVLLEPVFSFGEQNGPYFPYHFFSASDLYGPSYDNVSTINSMKEMIKRLHANGIEV 468

Query: 1335 LLEVVFTHTGEGG-TACQTISFRGIDNSSYYITEG----GMGSGMNCNNPIVQQLILDSL 1171
            LLEVVFTH  EGG ++ QTISFRGIDNSSYYI  G    G  + +NCNNPIVQ++ILD L
Sbjct: 469  LLEVVFTHAAEGGDSSFQTISFRGIDNSSYYILNGDTQLGTRNALNCNNPIVQRMILDIL 528

Query: 1170 HYWLTEFHVDGFCFINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMS 991
             YW+TEFH+DGFCF+N+S LLRG NG+ LS PPLVEAIAFDPLLS+TKIIADCW P +M 
Sbjct: 529  QYWVTEFHIDGFCFMNASSLLRGLNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPCEMV 588

Query: 990  HSDIHFPHWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYV 811
              DI FPHW+RWAE+N RFC D+R FLRGEGLLSDLATRLCGSGD+F D RGP FSFN++
Sbjct: 589  TRDIRFPHWKRWAEVNNRFCHDIRKFLRGEGLLSDLATRLCGSGDIFLDGRGPAFSFNFI 648

Query: 810  TKNFGLPLVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIP 631
             +NFGLPLVDLVSFS NE++SELSWNCGEEGATN  +VLE RLKQIRNFLFIL+IS G+P
Sbjct: 649  ARNFGLPLVDLVSFSGNEVSSELSWNCGEEGATNNTVVLERRLKQIRNFLFILYISFGVP 708

Query: 630  VFNMGDECGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDF 451
            + NMGDECG S+ G  +Y  R+P DW  L+T +  Q T+F+AFLSSLR RRSDL Q R+F
Sbjct: 709  ILNMGDECGQSSSGSTSYGDRKPFDWTALRTGFAIQTTEFIAFLSSLRTRRSDLLQKRNF 768

Query: 450  LKVKNIDWHGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSD----KGDLFVCFNGSDLP 283
            LKV+NI+W+GSNQS P+WEDPS KFLA+ L+++ +     SD    +GDLF+ FN     
Sbjct: 769  LKVENIEWYGSNQSQPRWEDPSSKFLALRLKSDIDNSQSDSDSSQIRGDLFIAFNAGGHS 828

Query: 282  ESVILPQLPEGYAWVCLVDTALPYPGFFSMDSN----FCDGLSTYELKPHSCTLFEA 124
            E VILP   EG  W+ LVDTALP+PGFFS D +       GL  YE+K HSC LFEA
Sbjct: 829  EGVILPSPSEGMVWLRLVDTALPFPGFFSNDGDPVLEQMQGLIAYEMKAHSCALFEA 885


>gb|OAY83378.1| Isoamylase 2, chloroplastic [Ananas comosus]
          Length = 1205

 Score = 1007 bits (2604), Expect = 0.0
 Identities = 508/837 (60%), Positives = 617/837 (73%), Gaps = 14/837 (1%)
 Frame = -2

Query: 2541 SEASHNSTEQIQPGLGLNYNA-EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSS 2365
            S AS +   ++ P +G+   + E+LQ+ FSY FRT+  G+VKV+V +   KYVV  EV+ 
Sbjct: 55   SSASRSPIRRLHPSVGVKIGSKEELQKGFSYTFRTDSNGIVKVVVRSEYGKYVVRFEVTF 114

Query: 2364 LPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTH 2185
            LP +  E  L+L+W MF SDSS L  P SQ S           G  + + PF QNS G+ 
Sbjct: 115  LPQFGTEEGLVLNWTMFTSDSSLLLVPCSQASE----------GTVTAKVPFVQNSSGSF 164

Query: 2184 AITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSE 2005
               L FD+++VP              +  S I++HRKTNFCVPVG+ SG+PMPLG S S+
Sbjct: 165  LAELVFDASKVPFYLSFLLN------SAGSEIRSHRKTNFCVPVGLGSGQPMPLGVSVSD 218

Query: 2004 DGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGY 1825
            DGL NF+LFS+ AE V LCLY+   ++P+L+IELDPYIN TGDIWHVS+E+V  YVSYGY
Sbjct: 219  DGLTNFSLFSKNAEGVILCLYDGIKDEPALEIELDPYINCTGDIWHVSLENVEEYVSYGY 278

Query: 1824 RCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVYLGY----LGDEPTFDWSGDV 1657
            RCKG I W  G RFH  HVLLDPYAK+I NF PD G  V L      L  E  FDWSGD 
Sbjct: 279  RCKGPILWRKGDRFHMKHVLLDPYAKVIGNFFPDHGGKVSLSRCLASLAKESAFDWSGDT 338

Query: 1656 HPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFP 1477
             P LPMEKLVVYRLNVG  TKDKSS L EN AGTF+G+IEK+++FK+LGVNAVLLEPIFP
Sbjct: 339  SPCLPMEKLVVYRLNVGLCTKDKSSGLSENAAGTFSGLIEKIEYFKTLGVNAVLLEPIFP 398

Query: 1476 YDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEGG 1297
            +DEK+GPYFP+HFFSPM SYG  RD  S   SMKEM+K++HA G+EVLLEVVF+HT EGG
Sbjct: 399  FDEKKGPYFPFHFFSPMMSYGHGRDSASGTNSMKEMIKSMHARGMEVLLEVVFSHTSEGG 458

Query: 1296 TAC-QTISFRGIDNSSYYITEGGMGSG----MNCNNPIVQQLILDSLHYWLTEFHVDGFC 1132
             A  Q ISFRGIDNSSYYI +G + SG    +NCNNPIVQ+LILDSLH+W+ E+HVDGFC
Sbjct: 459  DAASQMISFRGIDNSSYYIVDGDVRSGANNALNCNNPIVQRLILDSLHHWVVEYHVDGFC 518

Query: 1131 FINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDIHFPHWRRWA 952
            F+NSS L+R SNG+ LS PPL+EAIAFDP+LS+TKIIADCW P+DMS  +I FPHW++WA
Sbjct: 519  FVNSSSLVRSSNGNNLSRPPLLEAIAFDPILSKTKIIADCWSPIDMSSMEIQFPHWKKWA 578

Query: 951  EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 772
            E+N+RF  DVRNFLRGE LLSDLATR+CGSGDLFS SRGP +SFNY+TKNFGLPLVDLVS
Sbjct: 579  EMNSRFSVDVRNFLRGEALLSDLATRICGSGDLFS-SRGPAYSFNYITKNFGLPLVDLVS 637

Query: 771  FSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTG 592
            FSS +LASELSWNCGEEG T    VL+ RLKQIRNFLFILF+SLG+PV NMGDECG+S G
Sbjct: 638  FSSIDLASELSWNCGEEGPTGNSSVLQTRLKQIRNFLFILFVSLGVPVLNMGDECGYSNG 697

Query: 591  GLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQ 412
            G  +Y+ R+P DWN L+T +G QITQFVA+LSSLR RR D+FQ +DFLKV+NI W+GSNQ
Sbjct: 698  GSPSYNDRRPFDWNSLRTGFGLQITQFVAYLSSLRNRRGDIFQRKDFLKVENICWNGSNQ 757

Query: 411  SDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCL 232
            ++P W D SCKFL++  +A+ +G+     +GDLF+ FN SD PE  +LP+  EG  W+ L
Sbjct: 758  TEPNWGDSSCKFLSVMFKADADGRASKLHRGDLFIGFNASDHPEVALLPEQSEGTVWLRL 817

Query: 231  VDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEAGMSDL*FKVNLIEITSV 73
            VDTALP+PGFF+  S    +   GLS YE+K H    F    S   F+ N +   S+
Sbjct: 818  VDTALPFPGFFASYSDPNVHQVAGLSAYEIKSHRINTFAHTPSKEWFQTNAVLCVSL 874


>ref|XP_018683242.1| PREDICTED: isoamylase 2, chloroplastic-like [Musa acuminata subsp.
            malaccensis]
          Length = 862

 Score =  988 bits (2555), Expect = 0.0
 Identities = 508/848 (59%), Positives = 615/848 (72%), Gaps = 19/848 (2%)
 Frame = -2

Query: 2610 CRPGRKF-PEDATIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQ-RVFSYLFRTE 2437
            C   RKF P+ A+       N  +   S  S  Q +P    +  A+ L+ R F Y+FR+E
Sbjct: 34   CSCNRKFSPKGASFGPPTCLNSVVRATSPISLRQSRPMAVASGKAQVLEGREFPYMFRSE 93

Query: 2436 LGGLVKVLV-GANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLD 2260
             G LVKV+V GA  + + V+IEV S+    ++ +L+L + M RSD       D +     
Sbjct: 94   KGDLVKVVVVGAVGVNFSVHIEVLSV----SQDELVLIYEMSRSDPPGSLVADIEK---- 145

Query: 2259 TSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTH 2080
                    G  ++  PF +NS G + + L+FDSA+ P                 S IKTH
Sbjct: 146  --------GIGAVIMPFVRNSSGRYTLDLDFDSAKAPFYLSFMLHLCSDAGVVASEIKTH 197

Query: 2079 RKTNFCVPVGISSGRPMPLGFSKSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELD 1900
            RKT FCVPVG+  G P PLG S S+DG+VNF+LFSRKAE+V LCLYE     PSL+IELD
Sbjct: 198  RKTRFCVPVGLGPGYPAPLGASVSDDGMVNFSLFSRKAENVVLCLYEGKTEVPSLEIELD 257

Query: 1899 PYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQ 1720
            PY+NRTGDIWHVSMES+ +YVSYGYRCKG +    G  F   HVLLDPYAK+++N +  Q
Sbjct: 258  PYVNRTGDIWHVSMESIEDYVSYGYRCKGPVEKRGG--FDMQHVLLDPYAKMVRNLLSVQ 315

Query: 1719 GESVY----LGYLGDEPTFDWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTF 1552
            G+++     LG L  EP FDWSGDVHPQLP EKLVVYRLNVG FT D SS LPE+VAGTF
Sbjct: 316  GDTMTPTKCLGSLEMEPIFDWSGDVHPQLPTEKLVVYRLNVGQFTIDNSSGLPEDVAGTF 375

Query: 1551 AGVIEKLQHFKSLGVNAVLLEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKE 1372
             GV EK++HFK LGVNA+LLEPIFP+D  +GPYFPYHFFS M+ YG   +  S + SMKE
Sbjct: 376  GGVSEKVEHFKELGVNAILLEPIFPFDVNKGPYFPYHFFSVMDEYGQEHNAASAINSMKE 435

Query: 1371 MVKALHAHGIEVLLEVVFTHTGEGGTA-CQTISFRGIDNSSYYITEGGMGSGMN----CN 1207
            M+K LH+ GIEVL+EVVFTH+ EGG A    ISFRGID+SSYYI +  +GSG N    CN
Sbjct: 436  MIKTLHSEGIEVLMEVVFTHSAEGGDADSHVISFRGIDSSSYYIVDKNVGSGTNSLLKCN 495

Query: 1206 NPIVQQLILDSLHYWLTEFHVDGFCFINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETK 1027
            NPIVQQLI+DSL +W+ EFHVDGFCFINSS + RG NGD LS PPLVEAIAFDP+LS TK
Sbjct: 496  NPIVQQLIIDSLRHWVVEFHVDGFCFINSSSMARGQNGDHLSRPPLVEAIAFDPVLSRTK 555

Query: 1026 IIADCWCPVDMSHSDIHFPHWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFS 847
            I+ADCW P+DMS+ +I FPHW+ WAE+N RFC DVRNFLRGEGLLS+LATRLCGSGDLFS
Sbjct: 556  IVADCWSPLDMSYMEIQFPHWKAWAEMNMRFCSDVRNFLRGEGLLSNLATRLCGSGDLFS 615

Query: 846  DSRGPTFSFNYVTKNFGLPLVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRN 667
             SRGP FSFN+VTKN GLPLVDLVSFS+ ELASELSWNCG+EG TN   VLE RLKQIRN
Sbjct: 616  -SRGPAFSFNFVTKNVGLPLVDLVSFSTAELASELSWNCGDEGPTNNNTVLETRLKQIRN 674

Query: 666  FLFILFISLGIPVFNMGDECGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLR 487
            FLF+LF+SLG+PV NMGDECG+STGG   YD R+PI+W+ L T + KQIT+F+A+L SLR
Sbjct: 675  FLFVLFVSLGVPVLNMGDECGYSTGGSPLYDDRKPINWDSLGTGFSKQITKFIAYLGSLR 734

Query: 486  IRRSDLFQMRDFLKVKNIDWHGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFV 307
            IRR D+FQ + FLKV+NI W GSNQS+PKW+DP+CKFLA+ L++EKN  + +S+ GDLF+
Sbjct: 735  IRRGDIFQSKHFLKVENIVWFGSNQSEPKWDDPTCKFLALALKSEKNFDMSNSNCGDLFI 794

Query: 306  CFNGSDLPESVILPQLPEGYAWVCLVDTALPYPGFFSMDSNFCD-------GLSTYELKP 148
            CFN S+  E+V+LP+ PEG  W+ LVDT+L +PG F   SN CD       G S+YELKP
Sbjct: 795  CFNASNNLETVVLPEQPEGNVWLRLVDTSLAFPGIF---SNSCDPNVQKAEGSSSYELKP 851

Query: 147  HSCTLFEA 124
            HSC LFEA
Sbjct: 852  HSCALFEA 859


>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  965 bits (2495), Expect = 0.0
 Identities = 492/840 (58%), Positives = 607/840 (72%), Gaps = 15/840 (1%)
 Frame = -2

Query: 2586 EDATIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVG 2407
            ++AT +  +  N K+S  SH + E+ +        +E+L+   +YLF TE+GG VKV+VG
Sbjct: 57   QNATRNCFRNINWKVSATSHIAIEETENRYSTT-ESEELESRLNYLFLTEIGGQVKVIVG 115

Query: 2406 ANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRA 2227
              N KY+V+IEVSSL LY +++ LILSWG+FRS+SS     D Q  + +   +T+++   
Sbjct: 116  KKNKKYIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEI--- 172

Query: 2226 SIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGI 2047
                PF + S G+ A+ L+F++   P               + S I++HRKTNFC+PVG 
Sbjct: 173  ----PFMERSSGSFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGF 228

Query: 2046 SSGRPMPLGFSKSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWH 1867
              G P PLG S S DG  NFA FSR A  V LCLY+  +++P+L+I+LDPY+NRTGDIWH
Sbjct: 229  GRGYPAPLGLSFSSDGSPNFAFFSRNAGGVVLCLYDGTSDKPALEIDLDPYVNRTGDIWH 288

Query: 1866 VSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKN-FIPDQG--ESVYLGY 1696
             SMESVG++VSYGYRCK     D+G   H  HV LDPYAKLI+N F  D G      LG 
Sbjct: 289  ASMESVGSFVSYGYRCKEANLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGLKPQPRLGE 348

Query: 1695 LGDEPTFDWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKS 1516
            L  EP F+W+ DVHP +PMEKLVVYRLNV  FTKD+SS++  ++AGTF+G++EKL HFK 
Sbjct: 349  LQKEPAFNWNDDVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKD 408

Query: 1515 LGVNAVLLEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEV 1336
            LGVNAVLLEPIF +DE++GPYFP+HFFSPMN YG +   +ST+ S+KEMVK LHA+GIEV
Sbjct: 409  LGVNAVLLEPIFSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEV 468

Query: 1335 LLEVVFTHTGEGGTACQTISFRGIDNSSYYITEG----GMGSGMNCNNPIVQQLILDSLH 1168
            LLEVVFTHT E G      + +GID+S YY   G    G+ + +NCN  IVQQ+I+DSL 
Sbjct: 469  LLEVVFTHTAESG------ALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLR 522

Query: 1167 YWLTEFHVDGFCFINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSH 988
            YW+TEFHVDGFCFIN+S LLRG +G+ LS PPLVE IAFDPLLS+TKIIADCW P +M  
Sbjct: 523  YWVTEFHVDGFCFINASSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLP 582

Query: 987  SDIHFPHWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVT 808
             +I FPHW+RWAE+N RFC DVRNFLRGEGL SD ATRLCGSGD+F D RGP FSFN+ T
Sbjct: 583  KEIRFPHWKRWAEVNTRFCNDVRNFLRGEGL-SDFATRLCGSGDIFMDGRGPAFSFNFTT 641

Query: 807  KNFGLPLVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPV 628
            KNFGLPLVDLVSFSS+ELASELSWNCG+EG TNK  VLE RLKQIRNFLFIL++SLG+P+
Sbjct: 642  KNFGLPLVDLVSFSSSELASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPI 701

Query: 627  FNMGDECGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFL 448
             NMGDECG S+GG  AY  R+P +WN +KT +G Q  QF++FLSSLR RRSDL Q R FL
Sbjct: 702  LNMGDECGQSSGGSPAYGDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFL 761

Query: 447  KVKNIDWHGSNQSDPKWEDPSCKFLAMTLRAEK-NGKLP---SSDKGDLFVCFNGSDLPE 280
            K ++IDWHGS+QS P+W+DPS KFLAMTL+AE   G+LP   SS KGDLF+ FN +D   
Sbjct: 762  KEESIDWHGSDQSPPRWDDPSSKFLAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSV 821

Query: 279  SVILPQLPEGYAWVCLVDTALPYPGFFSMDSNFC----DGLSTYELKPHSCTLFEAGMSD 112
             VILP  P G  W  LVDTALP+PGFF+ D         GL TY+++ HSC LFEA   D
Sbjct: 822  KVILPPPPTGMVWHRLVDTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEANTLD 881


>gb|PIA39269.1| hypothetical protein AQUCO_02600010v1 [Aquilegia coerulea]
          Length = 886

 Score =  964 bits (2493), Expect = 0.0
 Identities = 486/826 (58%), Positives = 593/826 (71%), Gaps = 15/826 (1%)
 Frame = -2

Query: 2547 KISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVS 2368
            K+S  S  S+  I   L  + + E L+    YLFRT+ GG VKV VG  +MKY+V IEVS
Sbjct: 68   KVSALSQGSSYPIGTQLYKSADLEDLENASMYLFRTDAGGQVKVYVGRKHMKYIVYIEVS 127

Query: 2367 SLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGT 2188
             LP  +++ +L+LSWG++RSDSS +       + LD   STS    +  +TPF QNSLG 
Sbjct: 128  FLPQCVSKKELLLSWGVYRSDSSAM-------NPLDPQSSTSGAKSSISQTPFPQNSLGA 180

Query: 2187 HAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKS 2008
              + +EFDS Q P              +    IK+HRKTNFCVPVG+ SG P PLG S S
Sbjct: 181  RTLQMEFDSIQAPFYFSFLLLCPSSAASDLKEIKSHRKTNFCVPVGLGSGSPAPLGISFS 240

Query: 2007 EDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYG 1828
             DG VNFALFSR AE+V LCLY+    + SL+I+LDPYINRTGDIWHVSMESV  Y+SYG
Sbjct: 241  NDGSVNFALFSRNAENVVLCLYDEMEGKLSLEIDLDPYINRTGDIWHVSMESVAPYMSYG 300

Query: 1827 YRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESV----YLGYLGDEPTFDWSGD 1660
            YRC+G I  + G+  H SHVLLDPYAK++     DQ  SV    YLG L  EP+FDWSG+
Sbjct: 301  YRCRGTIPREKGNICHESHVLLDPYAKVLHRSFTDQSGSVPLLRYLGNLVKEPSFDWSGE 360

Query: 1659 VHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIF 1480
            + P LP+EKLVVYRLNV  FT+DKSS L   VAGTF+G+IEK+ HFK LGVNA+LLEPIF
Sbjct: 361  MRPCLPLEKLVVYRLNVECFTEDKSSRLSSAVAGTFSGLIEKMHHFKRLGVNAILLEPIF 420

Query: 1479 PYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEG 1300
             +DE++GPYFPYHFFSPMNSYG TRDG+S   SMKEMVK +H + IE+LLEVVFTHT E 
Sbjct: 421  SFDEQKGPYFPYHFFSPMNSYGPTRDGVSAASSMKEMVKVMHENNIEILLEVVFTHTAE- 479

Query: 1299 GTACQTISFRGIDNSSYYI----TEGGMGSGMNCNNPIVQQLILDSLHYWLTEFHVDGFC 1132
              A QTI  +GID+ SYY+     E    S +NCN PIVQ+LI+DSL YW+TEFHVDGFC
Sbjct: 480  -CAAQTIGLQGIDDKSYYVFDEDVESNDRSALNCNYPIVQRLIVDSLRYWVTEFHVDGFC 538

Query: 1131 FINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDIHFPHWRRWA 952
            FIN+S LLRG NG+ LS PPL+EAIAFDPLLS+TK+IAD   P      DI FPHW+RWA
Sbjct: 539  FINASSLLRGPNGEYLSRPPLIEAIAFDPLLSKTKVIADSLGPYSSDSKDIRFPHWKRWA 598

Query: 951  EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 772
            E+N +FC DVRNFLRGEGLLS+LATRLCGS D+F D RGP F+FN++ +NFGLPLVDLVS
Sbjct: 599  EMNVKFCSDVRNFLRGEGLLSNLATRLCGSADIFLDGRGPAFAFNFIARNFGLPLVDLVS 658

Query: 771  FSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTG 592
            FSS +L+SELSWNCGEEG TNK +VLE RLKQIRN LFIL++S+G+PV NMGDECG S+G
Sbjct: 659  FSSGKLSSELSWNCGEEGPTNKAVVLERRLKQIRNSLFILYLSMGVPVLNMGDECGQSSG 718

Query: 591  GLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQ 412
            G  +Y  R+  DW+ L T +G Q+T F++FLSS+R+RRSD  Q ++F++V++++WHGS+ 
Sbjct: 719  GSPSYSDRKSFDWDALSTTFGVQVTDFISFLSSVRMRRSDFLQRKNFMRVEDVEWHGSDL 778

Query: 411  SDPKWEDPSCKFLAMTLRAEKNGKLPSSDK---GDLFVCFNGSDLPESVILPQLPEGYAW 241
            + P WEDPS K LAMTL+A      P+S K    D F+  N +D  E+VILPQ  +G AW
Sbjct: 779  AQPSWEDPSNKLLAMTLKANLKSSKPNSKKSRGSDFFIALNANDSSETVILPQPSQGMAW 838

Query: 240  VCLVDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEAGMS 115
              LVDTALP+PGFF  D         G   YE+K HSC LFEA  S
Sbjct: 839  FRLVDTALPFPGFFRTDGEPVIEQTPGFLAYEMKSHSCALFEAHSS 884


>ref|XP_020592870.1| isoamylase 2, chloroplastic [Phalaenopsis equestris]
          Length = 861

 Score =  961 bits (2485), Expect = 0.0
 Identities = 495/866 (57%), Positives = 618/866 (71%), Gaps = 24/866 (2%)
 Frame = -2

Query: 2649 NIVSAKQVHPLSNC---RPGRKFPEDATIDAHKLRNVKISEASHNSTEQIQPGLGLNYNA 2479
            NI   + V+    C   R  R FP  A  D+ + R        +   E+ QP L      
Sbjct: 37   NISFIRSVYAQRRCINHRTHRTFPCGALNDSIRCR------IKNRLQEEAQPML------ 84

Query: 2478 EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSS 2299
            EK  + F +LFRTE GGLVKV VGA  MKY V IEV SLP  +  H+LIL WGMFRSDSS
Sbjct: 85   EKQHKGFKFLFRTEFGGLVKVFVGAMEMKYTVCIEVHSLPQSMINHNLILLWGMFRSDSS 144

Query: 2298 HLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXX 2119
                 DS+ S+            +S++TPF   S       L+FD + VP          
Sbjct: 145  VFQGSDSRISV------------SSMKTPFVGESC-----RLDFDHSNVPFYLSFLLLQP 187

Query: 2118 XXXLATDSGIKTHRKTNFCVPVGISSGRPMPLGFSKSEDGLVNFALFSRKAESVNLCLYE 1939
                  +S I+THRKTNFCVPVG+  G PMPLG S S DG  NF+LFSR A++V LCLYE
Sbjct: 188  DDT-GFESAIRTHRKTNFCVPVGLGPGHPMPLGVSLSNDGWTNFSLFSRNAKTVVLCLYE 246

Query: 1938 SGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLD 1759
            S  N+P+L++ELDPY+NRTGD+WHVS++++ NY+ YGYRCKG+I        HA HVLLD
Sbjct: 247  SETNEPTLELELDPYVNRTGDVWHVSIDNITNYILYGYRCKGDI--------HAHHVLLD 298

Query: 1758 PYAKLIKNFIP-DQGESVY----LGYLGDEPTFDWSGDVHPQLPMEKLVVYRLNVGSFTK 1594
            PYAK+I+N    D G+S      LGYL  +P FDWSG+  P +PMEKLVVYRLNV +FTK
Sbjct: 299  PYAKIIRNLSSSDHGDSESMVKSLGYLRKDPNFDWSGNTQPHIPMEKLVVYRLNVENFTK 358

Query: 1593 DKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIFPYDEKRGPYFP-------YHFF 1435
             +SS +  N+AGTFAGV +K+QH K+LG+NAVLLEP+FP+++K GPYFP       YHFF
Sbjct: 359  SESSGVDINIAGTFAGVAKKVQHLKNLGINAVLLEPVFPFNKKEGPYFPXXXXYFPYHFF 418

Query: 1434 SPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGE-GGTACQTISFRGIDN 1258
            SPM+  G        + S+KEMVK LH+HGIEVLLEVVFTHT E G +  + IS  GIDN
Sbjct: 419  SPMSMCG-------NMYSLKEMVKILHSHGIEVLLEVVFTHTCEVGDSESERISLLGIDN 471

Query: 1257 SSYYITEGGM----GSGMNCNNPIVQQLILDSLHYWLTEFHVDGFCFINSSYLLRGSNGD 1090
            SSYY+ +G +     S + CNN IV Q+ILDSLH+W+TE+H+DGFCF+NSS LL GSNG+
Sbjct: 472  SSYYVVDGNVISENCSRLKCNNAIVAQMILDSLHHWVTEYHIDGFCFVNSSSLLEGSNGN 531

Query: 1089 RLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDIHFPHWRRWAEINARFCRDVRNFL 910
             L  PPL+E IAFDPLLSETKII+DCW P+D+S+ D  FPHW+RWAE+N+++  D+RNFL
Sbjct: 532  HLPRPPLIEDIAFDPLLSETKIISDCWSPIDISYKDAIFPHWQRWAEMNSKYFHDIRNFL 591

Query: 909  RGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVSFSSNELASELSWNC 730
            RG+GL+S LATRLCGSGD+F+DSRGP  SFNY+T+N GL LVDLVS++ ++LASELSWNC
Sbjct: 592  RGQGLISQLATRLCGSGDMFTDSRGPGHSFNYITRNVGLTLVDLVSYNGSDLASELSWNC 651

Query: 729  GEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTGGLFAYDGRQPIDWN 550
            GEEG T++  VLE+RL+QIRNFLF+LF+SLG+PV NMGDECG S+ G  +Y+ + PIDW+
Sbjct: 652  GEEGLTDENSVLEIRLRQIRNFLFLLFVSLGVPVINMGDECGHSSSGSPSYN-KGPIDWS 710

Query: 549  GLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQSDPKWEDPSCKFLA 370
             L T + +QIT+FVAFL+SLR RRSD+FQ ++F K +NI WHGSNQS PKWEDPSCKFLA
Sbjct: 711  SLGTKFSEQITKFVAFLASLRDRRSDIFQRKEFYKAENIAWHGSNQSPPKWEDPSCKFLA 770

Query: 369  MTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEGYAWVCLVDTALPYPGFFSMD 190
            M L+AEK+ +LP+S  GDLF+  NG DL E+V LP+ PEG+ W  LVDT+ PYPGFFS D
Sbjct: 771  MELKAEKDVRLPNSHNGDLFIVVNGGDLLETVTLPESPEGFIWFRLVDTSRPYPGFFSSD 830

Query: 189  SN----FCDGLSTYELKPHSCTLFEA 124
            SN      +GLS+YELKPH+C LFEA
Sbjct: 831  SNPNSRKIEGLSSYELKPHACALFEA 856


>ref|XP_011624242.1| isoamylase 2, chloroplastic isoform X2 [Amborella trichopoda]
          Length = 891

 Score =  954 bits (2465), Expect = 0.0
 Identities = 472/826 (57%), Positives = 598/826 (72%), Gaps = 13/826 (1%)
 Frame = -2

Query: 2562 KLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVV 2383
            K+ + K+   S NS   I+  L        ++   +++FRTE+GG VKV+V   +M YV+
Sbjct: 66   KINDGKLFAISPNS---IEIALDKRIQTNVIEDRTTFMFRTEIGGQVKVVVSQKSMNYVI 122

Query: 2382 NIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQ 2203
            ++E+ SL     ++ L L WG+FRSDSS     DS+ S   T    S+ G   ++T F +
Sbjct: 123  SVELISLTDVCYQN-LELHWGIFRSDSSCWVLLDSENSPSGTDLVKSESGEDCMKTLFKK 181

Query: 2202 NSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPMPL 2023
               G H++ L FDS Q P                +S I+THRKTNF VPVGI  G P+PL
Sbjct: 182  TCSGEHSLELMFDSCQAPFYISFIVYSPFDSAWGNSQIRTHRKTNFVVPVGIGRGNPLPL 241

Query: 2022 GFSKSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGN 1843
            G S ++DG  NF+LFSR AE+V LCLY+    +P+L+IELDPYINR+GD+WHVS+ SV  
Sbjct: 242  GVSLNDDGSTNFSLFSRNAENVVLCLYDENTAKPALEIELDPYINRSGDMWHVSLSSVRQ 301

Query: 1842 YVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESV----YLGYLGDEPTF 1675
            Y+SYG+RCKG I WD G+R+H   +LLDPYAK++ NF  ++G SV     LG L  E  F
Sbjct: 302  YLSYGFRCKGAILWDKGNRYHMRRILLDPYAKILGNFNYNEGGSVPLVKCLGRLYTETAF 361

Query: 1674 DWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVL 1495
            DW GD  P +PMEKL+VYRLNVG FT+D SS LP+++AGTF GVI+KL H K+LGVNA+L
Sbjct: 362  DWDGDTSPCIPMEKLMVYRLNVGRFTEDMSSLLPKDIAGTFLGVIQKLHHLKNLGVNALL 421

Query: 1494 LEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFT 1315
            LEPIFP+DE +GPY+PY+FF+PMN YG  RDG+S   SMKEMVKALHA+G+EVLLE+VFT
Sbjct: 422  LEPIFPFDELKGPYYPYNFFAPMNKYGPLRDGISACTSMKEMVKALHANGLEVLLEMVFT 481

Query: 1314 HTGEGGTA-CQTISFRGIDNSSYYITEGGM----GSGMNCNNPIVQQLILDSLHYWLTEF 1150
            HT EGG + CQTISFRGIDNSSYYI +  +    G+ +NCN+P+VQ +ILD L +W+ E+
Sbjct: 482  HTAEGGDSLCQTISFRGIDNSSYYIVDRNVESEGGNVLNCNHPMVQTMILDCLRHWVHEY 541

Query: 1149 HVDGFCFINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDIHFP 970
            HVDGFCFINSS L +GS+G+ L+  PL+EAIAFDP+LS  KIIADCW P+DM   +IHFP
Sbjct: 542  HVDGFCFINSSSLAKGSDGELLTLSPLIEAIAFDPILSHAKIIADCWSPLDMQCKEIHFP 601

Query: 969  HWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLP 790
            HW++WAE+NARFC DVRNFLRGEGLLS+LATRLCGSGD+FSD RGP+FSFNY+ +NFGLP
Sbjct: 602  HWKKWAEMNARFCYDVRNFLRGEGLLSNLATRLCGSGDIFSDGRGPSFSFNYIARNFGLP 661

Query: 789  LVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDE 610
            LVDLVSFS +EL++ELSWNCGEEG T+  +VLE RLKQIRNFLFIL+ISLG+PV NMGDE
Sbjct: 662  LVDLVSFSGSELSAELSWNCGEEGPTSTPVVLESRLKQIRNFLFILYISLGVPVLNMGDE 721

Query: 609  CGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVKNID 430
             G STGG   Y  R+  DW  L+T +G Q TQ+V FLSSLR +RSDL Q + F+K++++D
Sbjct: 722  YGQSTGGSTLYSNRKSFDWGSLRTDFGVQTTQYVTFLSSLRTKRSDLLQRKHFMKIEHLD 781

Query: 429  WHGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEG 250
            WHG +QS P+WE PS KFLA+T+    +     S+ GDL+   N     E  +LPQ+   
Sbjct: 782  WHGEDQSQPQWEAPSSKFLAVTVNTGDDETETRSNGGDLYFAINAHGSSECAVLPQVSNN 841

Query: 249  YAWVCLVDTALPYPGFFSMDSNFCD----GLSTYELKPHSCTLFEA 124
             AW CLVDT+LPYPGFFSM+    D     ++ Y +KPHSCTLFEA
Sbjct: 842  MAWFCLVDTSLPYPGFFSMEGIPIDQPATSIAIYSMKPHSCTLFEA 887


>gb|ERN08315.1| hypothetical protein AMTR_s00156p00067780 [Amborella trichopoda]
          Length = 926

 Score =  954 bits (2465), Expect = 0.0
 Identities = 472/826 (57%), Positives = 598/826 (72%), Gaps = 13/826 (1%)
 Frame = -2

Query: 2562 KLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVV 2383
            K+ + K+   S NS   I+  L        ++   +++FRTE+GG VKV+V   +M YV+
Sbjct: 101  KINDGKLFAISPNS---IEIALDKRIQTNVIEDRTTFMFRTEIGGQVKVVVSQKSMNYVI 157

Query: 2382 NIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQ 2203
            ++E+ SL     ++ L L WG+FRSDSS     DS+ S   T    S+ G   ++T F +
Sbjct: 158  SVELISLTDVCYQN-LELHWGIFRSDSSCWVLLDSENSPSGTDLVKSESGEDCMKTLFKK 216

Query: 2202 NSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPMPL 2023
               G H++ L FDS Q P                +S I+THRKTNF VPVGI  G P+PL
Sbjct: 217  TCSGEHSLELMFDSCQAPFYISFIVYSPFDSAWGNSQIRTHRKTNFVVPVGIGRGNPLPL 276

Query: 2022 GFSKSEDGLVNFALFSRKAESVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGN 1843
            G S ++DG  NF+LFSR AE+V LCLY+    +P+L+IELDPYINR+GD+WHVS+ SV  
Sbjct: 277  GVSLNDDGSTNFSLFSRNAENVVLCLYDENTAKPALEIELDPYINRSGDMWHVSLSSVRQ 336

Query: 1842 YVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESV----YLGYLGDEPTF 1675
            Y+SYG+RCKG I WD G+R+H   +LLDPYAK++ NF  ++G SV     LG L  E  F
Sbjct: 337  YLSYGFRCKGAILWDKGNRYHMRRILLDPYAKILGNFNYNEGGSVPLVKCLGRLYTETAF 396

Query: 1674 DWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVL 1495
            DW GD  P +PMEKL+VYRLNVG FT+D SS LP+++AGTF GVI+KL H K+LGVNA+L
Sbjct: 397  DWDGDTSPCIPMEKLMVYRLNVGRFTEDMSSLLPKDIAGTFLGVIQKLHHLKNLGVNALL 456

Query: 1494 LEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFT 1315
            LEPIFP+DE +GPY+PY+FF+PMN YG  RDG+S   SMKEMVKALHA+G+EVLLE+VFT
Sbjct: 457  LEPIFPFDELKGPYYPYNFFAPMNKYGPLRDGISACTSMKEMVKALHANGLEVLLEMVFT 516

Query: 1314 HTGEGGTA-CQTISFRGIDNSSYYITEGGM----GSGMNCNNPIVQQLILDSLHYWLTEF 1150
            HT EGG + CQTISFRGIDNSSYYI +  +    G+ +NCN+P+VQ +ILD L +W+ E+
Sbjct: 517  HTAEGGDSLCQTISFRGIDNSSYYIVDRNVESEGGNVLNCNHPMVQTMILDCLRHWVHEY 576

Query: 1149 HVDGFCFINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDIHFP 970
            HVDGFCFINSS L +GS+G+ L+  PL+EAIAFDP+LS  KIIADCW P+DM   +IHFP
Sbjct: 577  HVDGFCFINSSSLAKGSDGELLTLSPLIEAIAFDPILSHAKIIADCWSPLDMQCKEIHFP 636

Query: 969  HWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLP 790
            HW++WAE+NARFC DVRNFLRGEGLLS+LATRLCGSGD+FSD RGP+FSFNY+ +NFGLP
Sbjct: 637  HWKKWAEMNARFCYDVRNFLRGEGLLSNLATRLCGSGDIFSDGRGPSFSFNYIARNFGLP 696

Query: 789  LVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDE 610
            LVDLVSFS +EL++ELSWNCGEEG T+  +VLE RLKQIRNFLFIL+ISLG+PV NMGDE
Sbjct: 697  LVDLVSFSGSELSAELSWNCGEEGPTSTPVVLESRLKQIRNFLFILYISLGVPVLNMGDE 756

Query: 609  CGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVKNID 430
             G STGG   Y  R+  DW  L+T +G Q TQ+V FLSSLR +RSDL Q + F+K++++D
Sbjct: 757  YGQSTGGSTLYSNRKSFDWGSLRTDFGVQTTQYVTFLSSLRTKRSDLLQRKHFMKIEHLD 816

Query: 429  WHGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSDKGDLFVCFNGSDLPESVILPQLPEG 250
            WHG +QS P+WE PS KFLA+T+    +     S+ GDL+   N     E  +LPQ+   
Sbjct: 817  WHGEDQSQPQWEAPSSKFLAVTVNTGDDETETRSNGGDLYFAINAHGSSECAVLPQVSNN 876

Query: 249  YAWVCLVDTALPYPGFFSMDSNFCD----GLSTYELKPHSCTLFEA 124
             AW CLVDT+LPYPGFFSM+    D     ++ Y +KPHSCTLFEA
Sbjct: 877  MAWFCLVDTSLPYPGFFSMEGIPIDQPATSIAIYSMKPHSCTLFEA 922


>ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber]
 ref|XP_023886632.1| isoamylase 2, chloroplastic [Quercus suber]
 gb|POE68275.1| isoamylase 2, chloroplastic [Quercus suber]
          Length = 887

 Score =  953 bits (2464), Expect = 0.0
 Identities = 485/830 (58%), Positives = 591/830 (71%), Gaps = 17/830 (2%)
 Frame = -2

Query: 2553 NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 2374
            N ++   S    E+I+  +     A+  ++  +YLF TE+GG VKV V   ++KY VNIE
Sbjct: 68   NSRVFATSRVLVEEIEQKVSTITEADNREKALTYLFWTEVGGQVKVFVRKTSVKYAVNIE 127

Query: 2373 VSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 2194
            VSSL L  +E  LI SWG +R+DSS     D Q+S+ D+   TS+       TPF Q S 
Sbjct: 128  VSSLQLQSSEDRLISSWGRYRADSSSFIPLDVQSSVPDSRTPTSE-------TPFIQTSA 180

Query: 2193 GTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 2014
            G  A+ LEF++ Q+P              +  S I++HRKT FCVPVG   G P PLG S
Sbjct: 181  GRFALELEFEAKQIPFYLSFILKLPSDSGSRGSEIRSHRKTKFCVPVGFRKGYPAPLGLS 240

Query: 2013 KSEDGLVNFALFSRKAESVNLCLYESG-ANQPSLDIELDPYINRTGDIWHVSMESVGNYV 1837
             S DG +NFA+FSR  E V LC Y+   A +P+L+++LDPYINR+GDIWH S ES   +V
Sbjct: 241  FSPDGSMNFAIFSRNVEGVILCFYDDAKAEEPALELDLDPYINRSGDIWHASFESAWTFV 300

Query: 1836 SYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQ-GESVYLGYLGDEPTFDWSGD 1660
            SYGY+CKG +   N ++F A  ++LDPYAK+I N IP   G   YLG L  EP FDW  D
Sbjct: 301  SYGYQCKGSLLQRNNNKFDAGQIILDPYAKIIVNSIPSSDGSQKYLGRLCKEPGFDWGDD 360

Query: 1659 VHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIF 1480
             HP LPMEKLVVYRLNV  FT+ KSS+L  +VAGTF G+ EKLQHFK LG+NAVLLEPIF
Sbjct: 361  THPYLPMEKLVVYRLNVRHFTEHKSSQLATDVAGTFIGLTEKLQHFKDLGMNAVLLEPIF 420

Query: 1479 PYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEG 1300
             +DEK GPYFP HFFSP N YG +   +S + SMKEMVK LHA+GIEVLLEVVFTHT   
Sbjct: 421  SFDEKNGPYFPCHFFSPTNLYGPSGGSVSAINSMKEMVKKLHANGIEVLLEVVFTHTAVA 480

Query: 1299 GTACQTISFRGIDNSSYYITEGGMG----SGMNCNNPIVQQLILDSLHYWLTEFHVDGFC 1132
            G      S +GID+ SYY    G      + +NCN PIVQQLILDSL YW+TEFH+DGFC
Sbjct: 481  G------SLQGIDDISYYHVNEGEDLEATNYLNCNYPIVQQLILDSLQYWVTEFHIDGFC 534

Query: 1131 FINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDIHFPHWRRWA 952
            FIN+S LLRG +G+ LS PPLVEAI+FDPLLS+TKIIADCW P DM   +  FPHW+RWA
Sbjct: 535  FINASSLLRGFHGEYLSRPPLVEAISFDPLLSKTKIIADCWDPHDMLPKETRFPHWKRWA 594

Query: 951  EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 772
            EI+ +FC DVRNF RGEGLLSDLATRLCGSGD+FSD RGP FSFN++ +NFGL LVDLVS
Sbjct: 595  EIDTKFCNDVRNFWRGEGLLSDLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVS 654

Query: 771  FSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFSTG 592
            FS++ LAS+LSWNCGEEG T+   VLE RLKQIRNFLF+L+ISLG+P+ NMGDECG S G
Sbjct: 655  FSNDALASQLSWNCGEEGPTDNTTVLERRLKQIRNFLFVLYISLGVPILNMGDECGQSCG 714

Query: 591  GLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSNQ 412
            G  AY  R+P+DWN LKT +  Q +QF++FLSSLR+RRSDL Q R+FLK +NI+WHGS+Q
Sbjct: 715  GSPAYSDRKPLDWNTLKTGFSIQTSQFISFLSSLRMRRSDLLQKRNFLKEENIEWHGSDQ 774

Query: 411  SDPKWEDPSCKFLAMTLRAEKNGKLPSSD------KGDLFVCFNGSDLPESVILPQLPEG 250
            S PKWEDPSC+FLAMTLRA++     +S+      +GDLF+ FN +DL ESVILPQ PEG
Sbjct: 775  SPPKWEDPSCRFLAMTLRADEVESSLNSESSSHVRRGDLFIAFNAADLSESVILPQPPEG 834

Query: 249  YAWVCLVDTALPYPGFFS-----MDSNFCDGLSTYELKPHSCTLFEAGMS 115
             +W CLVDTALP+PGFFS     +     + ++ YE+K HSC LFEA  S
Sbjct: 835  MSWRCLVDTALPFPGFFSTSGETLPEQMAESVA-YEMKSHSCALFEASSS 883


>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
          Length = 866

 Score =  944 bits (2440), Expect = 0.0
 Identities = 494/833 (59%), Positives = 594/833 (71%), Gaps = 14/833 (1%)
 Frame = -2

Query: 2580 ATIDAHKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGAN 2401
            AT D+ + RN+K+   S  S  Q            K ++  +YLFRTE+GG VKVLV   
Sbjct: 58   ATKDSFQHRNLKVLATSRISVGQ------------KEEKSSAYLFRTEIGGHVKVLVRQK 105

Query: 2400 NMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASI 2221
            N+KY V +EVSSL L   E  L+++WG+FRSDSS     D Q+S  D   ST       +
Sbjct: 106  NLKYSVYVEVSSLKLKSGEDKLVMNWGIFRSDSSQFIPLDLQSSTPDDRSST-------V 158

Query: 2220 RTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISS 2041
             TPF QNSLG   I L F+ +  P              +  S +++HRKT+FCVPVG++S
Sbjct: 159  ETPFVQNSLGKLVIELNFEGSLTPFYLSFLLKFPSDGDSKSSDLRSHRKTSFCVPVGLAS 218

Query: 2040 GRPMPLGFSKSEDGLVNFALFSRKAESVNLCLYE-SGANQPSLDIELDPYINRTGDIWHV 1864
            G P PLG S S DG +NFALFS+ A+ V LCLY+ + A +P+L+I+LDPY+NR+GDIWHV
Sbjct: 219  GYPAPLGLSFSADGSINFALFSQSADGVVLCLYDDTKAEKPTLEIDLDPYVNRSGDIWHV 278

Query: 1863 SMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVY--LGYLG 1690
            SM+S   +VSYGYRCKG I    G + H+ HVLLDPYAK+I N       SV   LG L 
Sbjct: 279  SMDSALPFVSYGYRCKGGIH-GKGVKDHSEHVLLDPYAKVIANA---HSGSVLKTLGKLI 334

Query: 1689 DEPTFDWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLG 1510
             EPTFDWSGD+ P +PMEKLVVYR+NV  FTK KSS LP +VAG+F G+ EKLQH K LG
Sbjct: 335  KEPTFDWSGDIRPCIPMEKLVVYRVNVAHFTKHKSSGLPNDVAGSFTGITEKLQHCKDLG 394

Query: 1509 VNAVLLEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLL 1330
            VNA+LLEP+FP+DEK+GPYFP+HFFSPM  YG + D  +TV SMKEMVK LHA+GIEVLL
Sbjct: 395  VNAILLEPVFPFDEKKGPYFPFHFFSPMKLYGPSDDPSATVNSMKEMVKILHANGIEVLL 454

Query: 1329 EVVFTHTGEGGTACQTISFRGIDNSSYYIT---EGGMGSGMNCNNPIVQQLILDSLHYWL 1159
            E+VFTHT E      + S R ID SSYYIT   + G  + +NCN PIVQQ+ILDSL YW 
Sbjct: 455  EIVFTHTAE------SCSLREIDKSSYYITGDEDLGAKNALNCNYPIVQQMILDSLRYWA 508

Query: 1158 TEFHVDGFCFINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDI 979
             EFHVDGFCFIN+S LL+G +G+ LS PPLVEAIAFDPLLS+TK+IAD W P DM+  +I
Sbjct: 509  IEFHVDGFCFINASSLLKGFHGEFLSRPPLVEAIAFDPLLSKTKLIADSWDPHDMASDEI 568

Query: 978  HFPHWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNF 799
             FPHW+RWAE+N  FC DVRNFLRGEGLLS+LATRLCGSGD+F + RGP FSFNY+T+N 
Sbjct: 569  LFPHWKRWAEVNTNFCGDVRNFLRGEGLLSNLATRLCGSGDMFLNGRGPGFSFNYITRNS 628

Query: 798  GLPLVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNM 619
            GLPLVDLVSFSS+ LASELSWNCGEEG TN   VLE RLKQIRN+LFILF+S G+PV NM
Sbjct: 629  GLPLVDLVSFSSSNLASELSWNCGEEGPTNNTAVLERRLKQIRNYLFILFVSFGVPVLNM 688

Query: 618  GDECGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVK 439
            GDECG S+GG  AY  R+P DWN L+T +G Q TQF++FLSSLR+RRSDL Q R FLK +
Sbjct: 689  GDECGQSSGGSPAYGDRKPYDWNALRTGFGIQTTQFISFLSSLRVRRSDLLQKRSFLKEE 748

Query: 438  NIDWHGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSD----KGDLFVCFNGSDLPESVI 271
            NIDW+GS+QS P W+D S +FLAMTL+ E+     SS+     GDLFV FN +D  E VI
Sbjct: 749  NIDWYGSDQSLPTWDDRSSRFLAMTLKVEREENESSSEFSSLNGDLFVAFNSADQSERVI 808

Query: 270  LPQLPEGYAWVCLVDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEA 124
            LP  P    W+ LVDTA+P+PGFFS D         GL TYE+K HSC LFEA
Sbjct: 809  LPPPPPEMVWLRLVDTAVPFPGFFSRDGEPVLEQMAGLVTYEMKSHSCVLFEA 861


>gb|OMO77794.1| hypothetical protein CCACVL1_14832 [Corchorus capsularis]
          Length = 878

 Score =  939 bits (2427), Expect = 0.0
 Identities = 480/852 (56%), Positives = 603/852 (70%), Gaps = 22/852 (2%)
 Frame = -2

Query: 2601 GRK-FPEDATIDA----HKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTE 2437
            GRK FP +    A    H   +++   AS  S EQ +         ++L+++ +Y+FRTE
Sbjct: 47   GRKLFPGEVVQSALQPPHFSLDLRCFAASRVSVEQTEQTFTSTSQVDELKKLSTYMFRTE 106

Query: 2436 LGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDT 2257
            +GG VK+ V   ++KYVV+IEVSSL L  + + L+LS G++RSD                
Sbjct: 107  IGGQVKIFVRKKSVKYVVDIEVSSLQLSGDNNKLVLSGGVYRSDH--------------- 151

Query: 2256 SQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHR 2077
                 DV   +I TPF   S    A+ LEF++ + P              ++   I++HR
Sbjct: 152  -----DVKTNNIETPFIARSSSELALELEFEAKEAPFYFSFLLKASSDANSSGLEIRSHR 206

Query: 2076 KTNFCVPVGISSGRPMPLGFSKSEDGLVNFALFSRKAESVNLCLYE-SGANQPSLDIELD 1900
            KTNFCVP+G   G P+PLG S S DG +NFA++SR AES+ LCLY+ +G+ +P+L+++LD
Sbjct: 207  KTNFCVPIGFDQGYPVPLGLSFSNDGSMNFAVYSRNAESLVLCLYDDTGSEKPALELDLD 266

Query: 1899 PYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQ 1720
            PY+NRTGDIWH S+E    +VSYGYRCKG+     G  FHA  VLLDPYAK+I + IP+ 
Sbjct: 267  PYVNRTGDIWHASLEGAWTFVSYGYRCKGD-----GDAFHAERVLLDPYAKIIGSSIPNH 321

Query: 1719 GES----VYLGYLGDEPTFDWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTF 1552
             ES     +LG L  EP FDWSGDV P LP+EKLVVYRLNV  FT+DKSS+LP NVAGTF
Sbjct: 322  YESGLLLKHLGRLCKEPAFDWSGDVCPNLPLEKLVVYRLNVMHFTEDKSSKLPANVAGTF 381

Query: 1551 AGVIEKLQHFKSLGVNAVLLEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKE 1372
            +GV EK+QH K LG+NAVLLEPIF +DE++GPYFP HFFSP + YG +   +S + S+K 
Sbjct: 382  SGVTEKVQHLKYLGINAVLLEPIFTFDEQKGPYFPCHFFSPTSLYGPSNGSISAINSIKG 441

Query: 1371 MVKALHAHGIEVLLEVVFTHTGEGGTACQTISFRGIDNSSYY----ITEGGMGSGMNCNN 1204
            MVK LHA+GIEVLLEVVFTHT EGG      + +G+D+ SYY    + +    + +NCN 
Sbjct: 442  MVKNLHANGIEVLLEVVFTHTAEGG------ALQGLDDESYYYRNRVEDLEEKNALNCNY 495

Query: 1203 PIVQQLILDSLHYWLTEFHVDGFCFINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKI 1024
            P+VQQ+ILDSL +W+TEFH+DGFCFIN+S LLRG +G+ LS PPLVEAIAFDPLLS+TK+
Sbjct: 496  PVVQQMILDSLRHWVTEFHIDGFCFINASCLLRGFHGEHLSRPPLVEAIAFDPLLSKTKV 555

Query: 1023 IADCWCPVDMSHSDIHFPHWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSD 844
            IADCW P +M   +IHFPHW+RWAE+N +FC D+RNFLRGE  LS LATRLCGSGD+FSD
Sbjct: 556  IADCWDPHEMMPKEIHFPHWKRWAEMNTKFCSDIRNFLRGEDALSSLATRLCGSGDIFSD 615

Query: 843  SRGPTFSFNYVTKNFGLPLVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNF 664
             RGP FSFN++ +NFGLPLVDLVSFS+ ELASELSWNCGEEG T+   VLE RLKQIRN+
Sbjct: 616  GRGPAFSFNFIARNFGLPLVDLVSFSNAELASELSWNCGEEGPTSNTAVLERRLKQIRNY 675

Query: 663  LFILFISLGIPVFNMGDECGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRI 484
            +FILF+SLG+PV NMGDECG S+GG  +Y  R+ +DWN + T +G Q TQF++FLSSLR+
Sbjct: 676  IFILFVSLGVPVLNMGDECGQSSGGSLSYGSRKLLDWNAMTTGFGIQTTQFISFLSSLRV 735

Query: 483  RRSDLFQMRDFLKVKNIDWHGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSD----KGD 316
            RRSDL Q R+FLK +NI+WHGS+QS P+WEDPSCKFLAMTL+A+K   L SS+    KGD
Sbjct: 736  RRSDLLQRRNFLKEENIEWHGSSQSPPEWEDPSCKFLAMTLKADKAESLLSSEASELKGD 795

Query: 315  LFVCFNGSDLPESVILPQLPEGYAWVCLVDTALPYPGFFSMDSNF----CDGLSTYELKP 148
            LF+  N  D  E++ILP  PEG AW  LVDTALPYPGFF  D         GL  YE+K 
Sbjct: 796  LFIAINADDKTENIILPPCPEGMAWRRLVDTALPYPGFFLADGKAVLEQMAGLVAYEMKS 855

Query: 147  HSCTLFEAGMSD 112
             SCTLFEA   D
Sbjct: 856  RSCTLFEACTED 867


>ref|XP_018858427.1| PREDICTED: isoamylase 2, chloroplastic [Juglans regia]
          Length = 889

 Score =  938 bits (2425), Expect = 0.0
 Identities = 481/827 (58%), Positives = 589/827 (71%), Gaps = 16/827 (1%)
 Frame = -2

Query: 2553 NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 2374
            N K+   S    ++ +  +      E + +  +YLF TE+GG VKV V   N+KY V +E
Sbjct: 64   NSKVYATSRVFIKETEQRVTTITEVEDMLKSLTYLFWTEIGGQVKVSVRKKNVKYAVYVE 123

Query: 2373 VSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIR-TPFTQNS 2197
            VSSL L+ ++  L+LSWG++R DSS     D+Q+S  D        GRA+ R TPF QN+
Sbjct: 124  VSSLQLHGSDDRLLLSWGIYRDDSSCFMHLDAQSSTPD--------GRATTRETPFIQNT 175

Query: 2196 LGTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPMPLGF 2017
             G   + L+F+  ++P               + S I++HRKTNFCVPVG  SG P PLG 
Sbjct: 176  KGRFVLELDFEEKKIPFYLSFLLKSSLGSDPSGSEIRSHRKTNFCVPVGFGSGYPAPLGL 235

Query: 2016 SKSEDGLVNFALFSRKAESVNLCLYESGA-NQPSLDIELDPYINRTGDIWHVSMESVGNY 1840
            + S DG +NF++FSR AESV LCLY+    ++P+L+++LDPY NR+GD+WH S+ES    
Sbjct: 236  TFSPDGSMNFSIFSRNAESVVLCLYDDMTIDEPTLELDLDPYTNRSGDVWHASLESAWTS 295

Query: 1839 VSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGESVYLGYLGDEPTFDWSGD 1660
            VSYGYRCKG +T  N     A  +LLDPYAK+I N IP    S YLG L  EP FDW  D
Sbjct: 296  VSYGYRCKGALTQRNKVNADAGEILLDPYAKIIGNSIPSNRGSGYLGRLCKEPAFDWGDD 355

Query: 1659 VHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPIF 1480
            VHP LPMEKL VYRLNV  FT+ KSS+LP++VAGTF+G+ EKLQHFK L +NAVLLEPIF
Sbjct: 356  VHPNLPMEKLAVYRLNVRHFTEHKSSQLPKDVAGTFSGLTEKLQHFKDLSMNAVLLEPIF 415

Query: 1479 PYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGEG 1300
             + E+ GPYFP HFFSP N YG + D MST+ SMKEMVK LHA+GIEVLLEV FTHT  G
Sbjct: 416  SFHEQNGPYFPCHFFSPTNQYGPSGDSMSTINSMKEMVKKLHANGIEVLLEVAFTHTAVG 475

Query: 1299 GTACQTISFRGIDNSSYYITEGGMGS----GMNCNNPIVQQLILDSLHYWLTEFHVDGFC 1132
            G      + +GID+ SYY + G   S     +NCN PIVQQLILDSL YW+TEFHVDGFC
Sbjct: 476  G------ALQGIDDLSYYYSNGVGDSEATNSLNCNYPIVQQLILDSLRYWVTEFHVDGFC 529

Query: 1131 FINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDIHFPHWRRWA 952
            FIN+S L+RG +G+ LS PPLVEAIAFDP LS+ KIIADCW P DM   +  FPHW++WA
Sbjct: 530  FINASSLMRGFHGEHLSRPPLVEAIAFDPFLSKIKIIADCWDPHDMLPKETRFPHWKKWA 589

Query: 951  EINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLVS 772
            EIN +FC DVRNF RGEGLLS LATRLCGSGD FSD RGP+FSFN+  ++FGL LVDLVS
Sbjct: 590  EINTKFCNDVRNFWRGEGLLSSLATRLCGSGDTFSDGRGPSFSFNFTARSFGLTLVDLVS 649

Query: 771  FSSNE-LASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFST 595
            FS+ + LAS LSWNCGEEG T+   VLE+RLKQIRNFLF+L+ISLG+P+ NMGDECG S+
Sbjct: 650  FSNTDALASHLSWNCGEEGPTDNTTVLEMRLKQIRNFLFVLYISLGVPILNMGDECGQSS 709

Query: 594  GGLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSN 415
            GG  AY  RQP DWN L+T +G Q TQF++FL+SLR RRSDL Q R FLK +NIDWHG++
Sbjct: 710  GGSLAYVDRQPFDWNALRTGFGIQTTQFISFLNSLRTRRSDLLQKRSFLKEENIDWHGND 769

Query: 414  QSDPKWEDPSCKFLAMTLRAEKNGKLP-----SSDKGDLFVCFNGSDLPESVILPQLPEG 250
            QS P WEDPSCKFLA+TL+A+K  K P     S  +GDLF+ FN +D  ESVILP+ PEG
Sbjct: 770  QSPPSWEDPSCKFLAVTLKADK-VKCPLNSESSHLRGDLFIAFNAADHSESVILPEPPEG 828

Query: 249  YAWVCLVDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEAG 121
             +W  LVDTALP+PGFFS++        +GL+ YE+K HS  LFEAG
Sbjct: 829  MSWRRLVDTALPFPGFFSINGEPIPEQMEGLAAYEMKSHSSALFEAG 875


>gb|OMP04997.1| hypothetical protein COLO4_09141 [Corchorus olitorius]
          Length = 868

 Score =  935 bits (2416), Expect = 0.0
 Identities = 478/852 (56%), Positives = 601/852 (70%), Gaps = 22/852 (2%)
 Frame = -2

Query: 2601 GRK-FPEDATIDA----HKLRNVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTE 2437
            GRK FP +    A    H   +++   AS  S EQ +         ++L+++ +Y+FRTE
Sbjct: 47   GRKLFPGEVVQSALQPPHLSLDLRCFAASRVSVEQTEQTFTSTSQVDELKKLSTYMFRTE 106

Query: 2436 LGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDT 2257
            +GG VK+ V   ++KYVV+IEVSSL L  N + L+LS G++RSD                
Sbjct: 107  IGGQVKIFVRKKSVKYVVDIEVSSLQLSGNNNKLVLSGGVYRSDH--------------- 151

Query: 2256 SQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHR 2077
                 D+   +I  PF   S    A+ LEF++ + P              +T   I++HR
Sbjct: 152  -----DIKTNNIEAPFIARSSSELALELEFEAKEAPFYLSFLLKASSDANSTGLEIRSHR 206

Query: 2076 KTNFCVPVGISSGRPMPLGFSKSEDGLVNFALFSRKAESVNLCLYE-SGANQPSLDIELD 1900
            KT+FCVP+G   G P+PLG S S DG +NFA++SR AES+ LCLY+ +G+ +P+L+++LD
Sbjct: 207  KTSFCVPIGFDQGYPVPLGLSFSNDGSMNFAVYSRNAESLVLCLYDDTGSEKPALELDLD 266

Query: 1899 PYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQ 1720
            PY+NRTGDIWH S+E    +VSYGYRCKG+     G  FHA  VLLDPYAK+I + IP+ 
Sbjct: 267  PYVNRTGDIWHASLEGAWTFVSYGYRCKGD-----GDAFHAERVLLDPYAKIIGSSIPNH 321

Query: 1719 GES----VYLGYLGDEPTFDWSGDVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTF 1552
             ES     +LG L  EP FDWSGDV P LP+EKLVVYRLNV  FT+DKSS+LP +VAGTF
Sbjct: 322  YESGLLLKHLGRLCKEPAFDWSGDVCPNLPLEKLVVYRLNVMRFTEDKSSKLPADVAGTF 381

Query: 1551 AGVIEKLQHFKSLGVNAVLLEPIFPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKE 1372
            +GV EK+QH K LG+NAVLLEPIF +DE++GPYFP HFFSP + YG +   +S + S+K 
Sbjct: 382  SGVTEKVQHLKYLGINAVLLEPIFTFDEQKGPYFPCHFFSPTSLYGPSNGSVSAINSIKG 441

Query: 1371 MVKALHAHGIEVLLEVVFTHTGEGGTACQTISFRGIDNSSYY----ITEGGMGSGMNCNN 1204
            MVK LHA+GIEVLLEVVFTHT EGG      + +G+D+ SYY    + +    + +NCN 
Sbjct: 442  MVKNLHANGIEVLLEVVFTHTAEGG------ALQGLDDESYYYRNRVEDLEEKNALNCNY 495

Query: 1203 PIVQQLILDSLHYWLTEFHVDGFCFINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKI 1024
            P+VQQ+ILDSL +W+TEFH+DGFCFIN+S LLRG +G+ LS PPLVEAIAFDPLLS+TK+
Sbjct: 496  PVVQQMILDSLRHWVTEFHIDGFCFINASCLLRGFHGEHLSRPPLVEAIAFDPLLSKTKV 555

Query: 1023 IADCWCPVDMSHSDIHFPHWRRWAEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSD 844
            IADCW P +M   +IHFPHW+RWAE+N +FC D+RNFLRGE  LS LATRLCGSGD+FSD
Sbjct: 556  IADCWDPHEMMPKEIHFPHWKRWAEMNTKFCSDIRNFLRGEDALSSLATRLCGSGDIFSD 615

Query: 843  SRGPTFSFNYVTKNFGLPLVDLVSFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNF 664
             RGP FSFN++ +NFGLPLVDLVSFS+ ELASELSWNCGEEG T+   VLE RLKQIRN+
Sbjct: 616  GRGPAFSFNFIARNFGLPLVDLVSFSNAELASELSWNCGEEGPTSNTAVLERRLKQIRNY 675

Query: 663  LFILFISLGIPVFNMGDECGFSTGGLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRI 484
            +FILF+SLG+PV NMGDECG S+GG  +Y  R+ +DWN + T +G Q TQF++FLSSLR 
Sbjct: 676  IFILFVSLGVPVLNMGDECGQSSGGSLSYGSRKLLDWNAMTTGFGIQTTQFISFLSSLRE 735

Query: 483  RRSDLFQMRDFLKVKNIDWHGSNQSDPKWEDPSCKFLAMTLRAEKNGKLPSSD----KGD 316
            RRSDL Q R+FLK +NI+WHGS+QS P+WEDPSCKFLAMTL+A+K   L SS+    KGD
Sbjct: 736  RRSDLLQRRNFLKEENIEWHGSSQSQPEWEDPSCKFLAMTLKADKAESLLSSEASELKGD 795

Query: 315  LFVCFNGSDLPESVILPQLPEGYAWVCLVDTALPYPGFFSMDSNF----CDGLSTYELKP 148
            LF+  N  D  E++ILP  PEG AW  LVDTALPYPGFF  D         GL  YE+K 
Sbjct: 796  LFIAINADDKTENIILPPCPEGMAWRRLVDTALPYPGFFLADGKAVLEQMAGLVAYEMKS 855

Query: 147  HSCTLFEAGMSD 112
             SCTLFEA   D
Sbjct: 856  RSCTLFEACTED 867


>ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]
 gb|ONH93660.1| hypothetical protein PRUPE_8G244800 [Prunus persica]
          Length = 883

 Score =  935 bits (2416), Expect = 0.0
 Identities = 479/826 (57%), Positives = 587/826 (71%), Gaps = 16/826 (1%)
 Frame = -2

Query: 2553 NVKISEASHNSTEQIQPGLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIE 2374
            N+K+   S  S E ++         E++ +V +YLFRTE+G +V V V   N KY VNIE
Sbjct: 68   NLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFVRKRNAKYTVNIE 127

Query: 2373 VSSLPLYLNEHDLILSWGMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSL 2194
            V S  L  N+  L+L WGM+R+DSS  + P      LD   ST +    ++ TP  Q S 
Sbjct: 128  VPSSHLSSNDRRLVLRWGMYRADSS-CFVP------LDFKSSTPNDTTTTLETPLIQTSS 180

Query: 2193 GTHAITLEFDSAQVPXXXXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPMPLGFS 2014
            G   + LEF++ Q+P               +D  I++HRKTNFCVPVG   G P PLG +
Sbjct: 181  GRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLT 240

Query: 2013 KSEDGLVNFALFSRKAESVNLCLYESG-ANQPSLDIELDPYINRTGDIWHVSMESVGNYV 1837
             S DG +NFA+FSR AESV LCLY++  A +P+L+++LDPY+NR+GDIWHVS  S  ++V
Sbjct: 241  FSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWSFV 300

Query: 1836 SYGYRCKGEITWDNGSRFHASHVLLDPYAKLIKNFIPDQGES--VYLGYLGDEPTFDWSG 1663
            SYGY+ KG +   N + F   HVLLDPYAK+I   IP+   +   YLG L +EP FDW+G
Sbjct: 301  SYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGLKYLGRLCEEPAFDWAG 360

Query: 1662 DVHPQLPMEKLVVYRLNVGSFTKDKSSELPENVAGTFAGVIEKLQHFKSLGVNAVLLEPI 1483
            DV P L MEKLVVYRLNV  FT+ KSS+LP N+ G+F+G+ EKL+HFK LGVNAVLLEPI
Sbjct: 361  DVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGVNAVLLEPI 420

Query: 1482 FPYDEKRGPYFPYHFFSPMNSYGCTRDGMSTVCSMKEMVKALHAHGIEVLLEVVFTHTGE 1303
            FP+DE++GPYFP+HFFSPM+ +G +R  +S V SMKEMV+  HA+GIEVLLEVVFTHT E
Sbjct: 421  FPFDEQKGPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLLEVVFTHTAE 480

Query: 1302 GGTACQTISFRGIDNSSYY----ITEGGMGSGMNCNNPIVQQLILDSLHYWLTEFHVDGF 1135
            G       + +GID SSYY    + +    + +NCN PIVQQL+LDSL YW+TEFHVDGF
Sbjct: 481  GE------ALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHVDGF 534

Query: 1134 CFINSSYLLRGSNGDRLSPPPLVEAIAFDPLLSETKIIADCWCPVDMSHSDIHFPHWRRW 955
             FIN+S LLRG NG+ LS PPLVEAIAFDPLLS+TKIIADCW P  M+  + HFPHWRRW
Sbjct: 535  FFINASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETHFPHWRRW 594

Query: 954  AEINARFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPTFSFNYVTKNFGLPLVDLV 775
            AE+N +FC DVRNFLRGEGLLSDLATRLCG+GD+FSD RGP F+FN++++N GLPLVDLV
Sbjct: 595  AEVNTKFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLV 654

Query: 774  SFSSNELASELSWNCGEEGATNKKLVLEVRLKQIRNFLFILFISLGIPVFNMGDECGFST 595
            SFS  ELASELSWNCGEEG TNK  VLE RLKQIRNFLFILF+SLG+PV NMGDECG ST
Sbjct: 655  SFSGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQST 714

Query: 594  GGLFAYDGRQPIDWNGLKTVYGKQITQFVAFLSSLRIRRSDLFQMRDFLKVKNIDWHGSN 415
            GG  AY  R+  DWN L+T +  Q TQF+AFLSS R RRSDL Q R+FLK +NI W+ S+
Sbjct: 715  GGSPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIGWYESD 774

Query: 414  QSDPKWEDPSCKFLAMTLRAEKN-----GKLPSSDKGDLFVCFNGSDLPESVILPQLPEG 250
            Q+ P+WEDPS KFLAM L+A+++     G   S   GDLFV F+ +D  E+V+LP   EG
Sbjct: 775  QTPPRWEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHSETVVLPPPLEG 834

Query: 249  YAWVCLVDTALPYPGFFSMDS----NFCDGLSTYELKPHSCTLFEA 124
              W  LVDTALP+PGFFS D         GL  YE+K HSC LFEA
Sbjct: 835  MGWRRLVDTALPFPGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEA 880