BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon25_contig00011123
         (4744 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020267901.1| E3 ubiquitin-protein ligase UPL4-like [Aspar...  1805   0.0  
ref|XP_020690782.1| E3 ubiquitin-protein ligase UPL4 [Dendrobium...  1313   0.0  
ref|XP_018682016.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...  1313   0.0  
gb|PNS98590.1| hypothetical protein POPTR_016G085200v3 [Populus ...  1217   0.0  
gb|PNS98589.1| hypothetical protein POPTR_016G085200v3 [Populus ...  1195   0.0  
ref|XP_022939346.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1096   0.0  
ref|XP_023005685.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1091   0.0  
ref|XP_023539461.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1089   0.0  
gb|PIA45462.1| hypothetical protein AQUCO_01700766v1, partial [A...  1087   0.0  
ref|XP_023527254.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1084   0.0  
ref|XP_022983502.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1082   0.0  
ref|XP_004137960.2| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...  1077   0.0  
ref|XP_022934795.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1077   0.0  
ref|XP_022934794.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1066   0.0  
ref|XP_022934791.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1066   0.0  
ref|XP_023766631.1| LOW QUALITY PROTEIN: E3 ubiquitin-protein li...  1030   0.0  
gb|PIA45461.1| hypothetical protein AQUCO_01700766v1 [Aquilegia ...  1004   0.0  
ref|XP_020102558.1| E3 ubiquitin-protein ligase UPL4-like [Anana...   949   0.0  
gb|PLY83359.1| hypothetical protein LSAT_1X53840 [Lactuca sativa]     941   0.0  
gb|PNS98591.1| hypothetical protein POPTR_016G085200v3 [Populus ...   880   0.0  

>ref|XP_020267901.1| E3 ubiquitin-protein ligase UPL4-like [Asparagus officinalis]
 gb|ONK67834.1| uncharacterized protein A4U43_C05F4270 [Asparagus officinalis]
          Length = 1400

 Score = 1805 bits (4675), Expect = 0.0
 Identities = 957/1405 (68%), Positives = 1100/1405 (78%), Gaps = 9/1405 (0%)
 Frame = -1

Query: 4618 EVFDLLPSTVNGLRSVGGDPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLA 4439
            +VF  LPS +  L+S   DPS  IA LTA CDALAFTQY DDLA  PL E+VP+L+EL A
Sbjct: 12   DVFGFLPSMIEVLKS-SDDPSELIANLTALCDALAFTQYEDDLAQFPLKEVVPVLIEL-A 69

Query: 4438 GRGGSPDVMLMSARVLTYFLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEAL 4259
            G+G SPD M +  RV TY LDL+PRAA + L K GA+  LCGPLME+ YLDVAEQCL AL
Sbjct: 70   GKGVSPDAMSLVVRVFTYLLDLVPRAA-KVLAKKGAIAVLCGPLMEIEYLDVAEQCLVAL 128

Query: 4258 DKLSRTQSVPCLRAGVINLVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPM 4079
            +KLSR Q+VPCL+AGVI  VL F+DFF++  QR+A+S  +N C++VPEDC S VMDAVP 
Sbjct: 129  EKLSRRQTVPCLKAGVIQTVLRFIDFFDIRVQRVAISIAMNACRKVPEDCLSTVMDAVPT 188

Query: 4078 LCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSE 3899
            LC LLDYQD+KLVES A  LM+IVENF HSS+LLDELCKHGVVKQSVKLISP S +ALSE
Sbjct: 189  LCKLLDYQDQKLVESAATSLMKIVENFSHSSDLLDELCKHGVVKQSVKLISPCSTVALSE 248

Query: 3898 TTYIGMIELLVRLANRSLLSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVY 3719
             T IGMIELL RLAN SLL++  LYE NINSIL ++LR P  S     SH ED SRDQ++
Sbjct: 249  RTNIGMIELLARLANSSLLAVSILYELNINSILGNLLRRPHLSHSAHNSHSEDDSRDQIF 308

Query: 3718 VALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACI 3539
             ALKLLNQLIPP+A D++DIQLV GK +II +EPKLSFQFA  ILPAL QAV SGA+  I
Sbjct: 309  AALKLLNQLIPPMACDEKDIQLVSGKNEIIANEPKLSFQFAEDILPALIQAVNSGASTRI 368

Query: 3538 HYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLP 3359
             Y  VSVI NY L  TPD+LR LIKNAN+PKFLA LLS+K+HH+LIP+L +VE+IM++LP
Sbjct: 369  CYGSVSVILNYFLAVTPDVLRVLIKNANIPKFLASLLSQKNHHLLIPSLLIVEVIMRELP 428

Query: 3358 GELLNSFVKEGVSHAIRVLSKQDGCYQSQLMDQQSSNLGDTDERTAANLSRCLCYAFD-X 3182
            GELL  FVKEGVSHAI V+SKQ+   Q        ++ GDTD++T AN  RCLCYAFD  
Sbjct: 429  GELLKPFVKEGVSHAINVISKQENWSQ--------TDTGDTDDQTIANPPRCLCYAFDPC 480

Query: 3181 XXXXXXXSKMCNLPRHTLLPLAQRVMSTFFXXXXXXXXXXXXXLQKLKACYIALDKNVDS 3002
                   S+ C   R  L PLAQR+MSTFF             L+KLK+C + +D NV S
Sbjct: 481  SSPSSSESRTCRQRRCDLSPLAQRLMSTFFTETLISSMVFSENLRKLKSCCMEIDNNVHS 540

Query: 3001 ALTNCAEREDYLSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGD 2822
            AL N +E+E+YL+QM+ E V  LNEG+S+STFEFIESG  RFL HYLTNGD+  R +  +
Sbjct: 541  ALPNSSEKEEYLTQMLGELVKVLNEGESVSTFEFIESGIPRFLVHYLTNGDHFPRGVYSN 600

Query: 2821 MSFDYLTVLKRLQTFSFKLLSGHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSR 2642
             +  Y TVLKR QTF+  LLSG S Q FPL SLV+HLQNALS+LE FP++LS+  +   R
Sbjct: 601  KTEAYHTVLKRFQTFTSMLLSGQSWQLFPLTSLVRHLQNALSSLESFPIILSSGYRPTPR 660

Query: 2641 STDIPAKSSTKHPCLQVHFVTEEGETDLCLYDNVLTVDISSSYDAIEEFLWPXXXXXXXX 2462
              DI A+  T HPCLQV+FVTEE ET+LC Y+NVLTVD SSS+D IEEFLWP        
Sbjct: 661  RADISAEKPTVHPCLQVNFVTEERETELCNYNNVLTVDFSSSWDTIEEFLWPKVKKKMSV 720

Query: 2461 XXXXXXTQRHIKQSSRKVDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAIL 2282
                  T+   ++S++ VDS+E+  +S  QNNE D++PKL+FLLDGKQLD S+TLYQAIL
Sbjct: 721  TKSAQETRHPGEKSAQIVDSQEQGENSMLQNNE-DVKPKLVFLLDGKQLDHSVTLYQAIL 779

Query: 2281 QDKINEDPDIIVSKKFWNEVHRVTLWSAKKLQLIYP------QISADPRQSTLSWDKTEL 2120
            QDKIN+DP II++++FWNEVH V    AK+LQL  P      Q+ AD  QS+LS D   L
Sbjct: 780  QDKINQDPAIIINREFWNEVHGVKFRKAKELQLRLPLKKGSFQVFADTSQSSLSCDTAGL 839

Query: 2119 PWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGT 1940
                N SFFSSMLL+ELP KLERSNPAYD+L++LKILEG+N  SFQL SNE+SNAFAEG 
Sbjct: 840  -CRQNLSFFSSMLLSELPCKLERSNPAYDILYILKILEGINGFSFQLSSNEKSNAFAEGR 898

Query: 1939 LENFDDLKVIAHSIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSF 1760
            L N DDLKVIA S+PQTEFI+SKL SKLEQQMHD+L LST TMPLWC Q++V+ PFLFS 
Sbjct: 899  LNNADDLKVIAPSLPQTEFISSKLNSKLEQQMHDHLVLSTTTMPLWCTQIMVSFPFLFSL 958

Query: 1759 EARWKYFCLNIFGVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMM 1580
            EARWKYF LN  G+SRN  QNSEKNEIS INDRRSLS SL RKKFKVHR+NIL SA KMM
Sbjct: 959  EARWKYFFLNTLGISRNHMQNSEKNEISNINDRRSLSTSLRRKKFKVHRDNILGSAVKMM 1018

Query: 1579 QWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGT--S 1406
             WYA KKVLLEI F+NEVGTGLGPTLEFYTLVSH  QKVGMGMWRGDHTS+  +L +  S
Sbjct: 1019 NWYASKKVLLEIVFNNEVGTGLGPTLEFYTLVSHELQKVGMGMWRGDHTSSGTKLHSRKS 1078

Query: 1405 MVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPF 1226
            MVVSDS F+  HSGLFPRPWS  ++  NGVSFSKVLKNFFLLGQL ARAIQDGRILD  F
Sbjct: 1079 MVVSDSSFVRAHSGLFPRPWSIRVESLNGVSFSKVLKNFFLLGQLVARAIQDGRILDFTF 1138

Query: 1225 SRAFYKLMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHN 1046
            SRAFYKL+LEKDLDIYDIQSFDPELGRT+IEFQAL KRK FLE  SGE S D     F N
Sbjct: 1139 SRAFYKLVLEKDLDIYDIQSFDPELGRTLIEFQALVKRKAFLE--SGELSEDGSALSFRN 1196

Query: 1045 TRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSG 866
             RIE+LCLDF+LPGYPDY F S  DSKMVN+SNL EY+SLVVDATV SGISRQLESFKSG
Sbjct: 1197 ARIEDLCLDFALPGYPDYAFGS-SDSKMVNMSNLGEYISLVVDATVGSGISRQLESFKSG 1255

Query: 865  FSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQE 686
            F++VFPIKALQIFTA+ELE+++ GEQDTWD  E Q+HI FDHGYSSTS  A+ FLE+IQE
Sbjct: 1256 FNEVFPIKALQIFTAQELEQIVSGEQDTWDNNEFQDHIKFDHGYSSTSPTAIMFLEVIQE 1315

Query: 685  FDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPP 506
            F+ RQRRAFLLFVTGAPRLPPGGLAAL+PKLTIVCKHCDNDADMELPSVMTCANYLKLPP
Sbjct: 1316 FEVRQRRAFLLFVTGAPRLPPGGLAALRPKLTIVCKHCDNDADMELPSVMTCANYLKLPP 1375

Query: 505  YSSKERMREKLLYAIREGQGAFHLS 431
            YSSKERM+EKLLYAI EGQGAFHLS
Sbjct: 1376 YSSKERMKEKLLYAIMEGQGAFHLS 1400


>ref|XP_020690782.1| E3 ubiquitin-protein ligase UPL4 [Dendrobium catenatum]
 ref|XP_020690783.1| E3 ubiquitin-protein ligase UPL4 [Dendrobium catenatum]
 gb|PKU64336.1| E3 ubiquitin-protein ligase UPL4 [Dendrobium catenatum]
          Length = 1522

 Score = 1313 bits (3398), Expect = 0.0
 Identities = 712/1435 (49%), Positives = 959/1435 (66%), Gaps = 48/1435 (3%)
 Frame = -1

Query: 4591 VNGLRSVGGDPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVM 4412
            ++ L S    PSAQ+A LT  C+AL+F    D L   P+D  +PLLV L AG  G+PDVM
Sbjct: 101  ISVLDSEDAGPSAQLASLTELCEALSFCM-EDSLGYFPMDNALPLLVRL-AGSEGNPDVM 158

Query: 4411 LMSARVLTYFLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSV 4232
            L++ R +TY  D+MPRAAD A+++HGA+  LCG L+ + YLDVAEQ L+AL+K+SR Q V
Sbjct: 159  LLAIRAITYLCDVMPRAAD-AIVRHGALSVLCGRLLSIEYLDVAEQSLQALEKISRKQPV 217

Query: 4231 PCLRAGVINLVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQD 4052
            PCL+AG I  VL FMDFF  S QR+ALSTV NVCK++P DC S+V++AVP LCNLL Y+D
Sbjct: 218  PCLQAGTIMAVLGFMDFFSTSVQRVALSTVANVCKKLPLDCSSLVLEAVPTLCNLLQYED 277

Query: 4051 RKLVESTAMCLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIEL 3872
             KLVE+   CLMRI   F  S+E+LD LCKHGV+ +S+ LI+      LS++ ++G+I L
Sbjct: 278  CKLVETAVTCLMRITSCFSSSTEILDVLCKHGVISKSLHLITVDGRTTLSQSAFVGLIGL 337

Query: 3871 LVRLANRSLLSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQL 3692
            L  LAN SL+++R+L+E  ++S L+ IL     S  T  S+ + +  +QV+  LKLLN +
Sbjct: 338  LANLANASLVAVRTLFELGVSSSLKRILIASGISHGTSYSYPDGVYSNQVHEVLKLLNHM 397

Query: 3691 IPPVARDDQDIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIK 3512
            +P  ARDD++IQLV+ KE I+ ++P    QF+  ILP L + V SGAN C+ Y C+SV+ 
Sbjct: 398  MPSAARDDENIQLVMAKEMILEEKPMFLHQFSADILPVLIEVVKSGANLCVCYGCLSVMI 457

Query: 3511 NYVLIATPDMLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVK 3332
            N +  +T DML+ L+KN+N+  FLAGL++RKD HVL+ AL +VE++M+K      +SF+K
Sbjct: 458  NIIYFSTVDMLQDLVKNSNISSFLAGLMARKDRHVLVSALKIVELLMQKFSCVCSSSFIK 517

Query: 3331 EGVSHAIRVLSKQDGCYQSQ--LMDQQSSNLGDTDERTAANLSRCLCYAFDXXXXXXXXS 3158
            EGV +AI VL + +   +S    +DQQ + +   D      + RCLCY FD         
Sbjct: 518  EGVVYAIEVLLEHENRLESVGLRLDQQDNQIALRD------VPRCLCYVFDPPNTSTSER 571

Query: 3157 KMCNLPRHTLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTN--C 2987
            K C L +  +  LA+ + +T+F             + +KLK     L++ VD +  N  C
Sbjct: 572  KACRLGKDAVFSLAKHIKATYFTGESVNCEIGVSEVLKKLKIMCATLNETVDQSSFNDGC 631

Query: 2986 AEREDYLSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGDMSFDY 2807
            A+ E+ LSQ++ + +  +N G++IS+FEF+ESG IR L HYL+NG YL+++    MS  +
Sbjct: 632  AKTEE-LSQLLGQVMAEINLGEAISSFEFVESGIIRSLVHYLSNGRYLNKSYSDCMSTSH 690

Query: 2806 -LTVLKRLQTFSFKLLSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRST 2636
             L +LKRLQ F+   LS    + +   L  LV+ L +AL++ + FPV++S   KS+S  +
Sbjct: 691  WLAILKRLQIFTGISLSKDCQNWEDMLLTLLVRKLLDALTSFDNFPVIVSQFFKSRSSYS 750

Query: 2635 DIPAKSSTKHPCLQVHFVTEEGETDLCLYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXX 2456
            DIP++ ST HPCL+V FV E+GE +L  Y +V+++++SSS+ AIE FLWP          
Sbjct: 751  DIPSRHSTLHPCLRVRFVREDGELNLSDYSDVVSIEVSSSFVAIEGFLWPKVGASMSGQL 810

Query: 2455 XXXXT------------------------QRHIKQSSRKV--------------DSKERV 2390
                                         Q  ++ +S K+                    
Sbjct: 811  QEQKGKDVTTTSIAVSSVRCPEVLINQEQQASLEDTSMKLREMMTESSSDPHSSSEGHAK 870

Query: 2389 GSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVT 2210
            GS ++  +  D  PKLIF ++G+++DRS+TLYQAILQ +IN +PD++V  KFWNEV++V+
Sbjct: 871  GSESSFPSNKDATPKLIFSMEGREVDRSLTLYQAILQFQINAEPDLVVGPKFWNEVYKVS 930

Query: 2209 LWSAKKLQLIYPQISADPRQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDM 2030
               A +    + Q +     S +  +K   PWH   SF SS+ L ELP KL++SNP+Y++
Sbjct: 931  YRKASQ-NTTHRQEACCDSPSLIFQEKAGYPWHK-LSFISSIFLAELPCKLDKSNPSYEL 988

Query: 2029 LFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAHSIPQTEFINSKLTSKLEQ 1850
            LF++KILEGLNR S  L S ER  + AEG +EN DDLKV+  SIPQ EFI+ KLT KLEQ
Sbjct: 989  LFMMKILEGLNRFSSYLLSYERCTSLAEGRIENLDDLKVVVPSIPQAEFISCKLTDKLEQ 1048

Query: 1849 QMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSR--NQTQNSEKNEIS 1676
            Q+ D L L+T  MP WC QL++ACPFLFSFEARWKYF    FG     NQ Q +  +  S
Sbjct: 1049 QLQDPLTLATGCMPAWCGQLMMACPFLFSFEARWKYFRFTTFGCKGQLNQIQPANISGSS 1108

Query: 1675 TINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEF 1496
            ++ DRRS S    RKK KV R++IL SAAKMM  +A  + ++E+E+  EVGTGLGPT+EF
Sbjct: 1109 SVIDRRSASVWSYRKKIKVTRSDILGSAAKMMASHAQSRAVIEVEYEEEVGTGLGPTMEF 1168

Query: 1495 YTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGV 1316
            +TLVSH FQK GMGMWRGD +S      TS    +S F++   GLFP PW+     S+  
Sbjct: 1169 FTLVSHEFQKAGMGMWRGDKSSFNVSQ-TSQCSDESAFVVAPFGLFPCPWAAAACASDDR 1227

Query: 1315 SFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEKDLDIYDIQSFDPELGRTMI 1136
             FS V+K F LLGQL A+AI+DGRILD+PF  AFYK++L+++L IYDIQSFDPELGRT++
Sbjct: 1228 QFSDVIKKFTLLGQLVAKAIRDGRILDIPFCSAFYKVILDQELGIYDIQSFDPELGRTLL 1287

Query: 1135 EFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEELCLDFSLPGYPDYVFSSIGDSKMVN 956
            EFQAL+ RK FLES S E+     D  F +T IE+LCLDF+LPGY DYV SS  +S+M+N
Sbjct: 1288 EFQALSYRKRFLESASTENFEYASDLCFRSTAIEDLCLDFTLPGYSDYVLSSATNSEMLN 1347

Query: 955  ISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWD 776
            I NLEEYVSLVVDAT++ GISRQ+++F+SGF++VFP+K+LQIFT +ELER+LCGEQ+TW 
Sbjct: 1348 IHNLEEYVSLVVDATIKDGISRQVQAFRSGFNEVFPLKSLQIFTEDELERVLCGEQETWT 1407

Query: 775  IIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPK 596
              EL +H+ FDHGY+++S P +  LE IQEF+  +RRAFL FVTGAPRLPPGGLAAL PK
Sbjct: 1408 FSELLDHVKFDHGYTASSPPVINLLETIQEFECNERRAFLQFVTGAPRLPPGGLAALNPK 1467

Query: 595  LTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 431
            LTIV K C  + DM+LPSVMTCANYLKLPPYSSKE  RE+LLYAI EGQG+FHLS
Sbjct: 1468 LTIVRKLCSYEVDMDLPSVMTCANYLKLPPYSSKEITRERLLYAITEGQGSFHLS 1522


>ref|XP_018682016.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X2 [Musa
            acuminata subsp. malaccensis]
          Length = 1505

 Score = 1313 bits (3397), Expect = 0.0
 Identities = 733/1446 (50%), Positives = 956/1446 (66%), Gaps = 55/1446 (3%)
 Frame = -1

Query: 4603 LPSTVNGLRSVGGDPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGS 4424
            L    +GL   G   SAQ+A LT  C+ L+F    D +   PL+ +VP LV+L A    S
Sbjct: 85   LQRVFSGLLDDGSGGSAQLAALTELCEVLSFCM-EDAVGYFPLETVVPPLVKL-ASHESS 142

Query: 4423 PDVMLMSARVLTYFLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSR 4244
            PDVML++ R LTY  D MPR+A+ A+++HGA+  LCG L+ + YLDVAEQ L+AL+K+SR
Sbjct: 143  PDVMLLAIRALTYLCDAMPRSAE-AIVRHGALPVLCGRLLAIEYLDVAEQSLQALEKISR 201

Query: 4243 TQSVPCLRAGVINLVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLL 4064
             Q VPCL+AG I  VL+++DFF  + QR+A+STV NVCK++P DC +IVM++VP+LC+LL
Sbjct: 202  KQPVPCLQAGTIAAVLTYIDFFPTNPQRVAVSTVANVCKKLPPDCSTIVMESVPILCSLL 261

Query: 4063 DYQDRKLVESTAMCLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIG 3884
             Y+D KLVE+ A CL+RI + F  SSELLDELCKHG++++S+ LI+     +LS  TY G
Sbjct: 262  QYEDHKLVETVAACLVRITDCFAGSSELLDELCKHGIIQKSLNLIANDGHRSLSRATYSG 321

Query: 3883 MIELLVRLANRSLLSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKL 3704
            +I LL +LA  SL+++++L+E NI+  L  IL + D  R +     +D+  +QVY  LKL
Sbjct: 322  LIGLLRKLATSSLVAVQTLFELNISRTLMGILMSSDMLRDSAYVSVQDMQTNQVYEVLKL 381

Query: 3703 LNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACV 3524
             NQLIPPV RD  D Q+ L KEKI++D+P    +F+  ILP   Q V SGANA + YACV
Sbjct: 382  ANQLIPPVLRDVPDDQIELAKEKILVDQPNFLHEFSTDILPVSVQVVNSGANAYVCYACV 441

Query: 3523 SVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLN 3344
            S+I +    +TPD+L   IK+ N+  FLAGLLSRKD HV+   L  VE++M+KLP   L+
Sbjct: 442  SIINSIAYFSTPDILLDSIKSTNISSFLAGLLSRKDPHVIFLTLKTVEVLMQKLPAVFLS 501

Query: 3343 SFVKEGVSHAI-RVLSKQDGCYQSQLMDQQSSNLGDTDERTAANLSRCLCYAFDXXXXXX 3167
            SF+KEGV +AI   L  Q+ C  S     + SN    D     + SRC+C+AF+      
Sbjct: 502  SFIKEGVVYAIDAALLVQEKCSDSV---SEHSN----DHMVVRDTSRCMCHAFNSSRVSA 554

Query: 3166 XXSKMCNLPRHTLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTN 2990
              SK C L + ++  LA+ + +T+F               QKLK     L+ NVDS  T+
Sbjct: 555  SESKTCRLQKDSIQSLARHIKTTYFTHEAVDSEMGFTETLQKLKILCTVLNDNVDSCSTS 614

Query: 2989 --CAEREDYLSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDG-DM 2819
              C + E+ L+Q++++ +   +EG+S+STFEFIESG  RFLA YL+NG YL       D+
Sbjct: 615  DGCLQNEENLTQILLQVMREFSEGESMSTFEFIESGIARFLACYLSNGKYLSGTTSAIDL 674

Query: 2818 SFDYLTVLKRLQTFSFKLLS--GHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKS 2645
            S   LTVLKR Q FS   LS  G S  +  LA L++  QNALS+L+ FPV+LS   K ++
Sbjct: 675  SSHILTVLKRFQIFSSICLSNPGQSCDNMLLAVLLKKFQNALSSLDNFPVILSHGFKLRN 734

Query: 2644 RSTDIPAKSSTKHPCLQVHFVTEEGETDLCLYDNVLTVDISSSYDAIEEFLWPXXXXXXX 2465
              TDIP +  TK+PCL+V FV +  +T+L   DNV+ VDISSS+DA+E +LWP       
Sbjct: 735  TYTDIPVRGITKNPCLRVRFVRQNEDTNLSDLDNVVNVDISSSFDALEGYLWPKVNKGKN 794

Query: 2464 XXXXXXXTQRH------IKQSSRK-------------------------------VDS-- 2402
                    ++       IK  S K                               VDS  
Sbjct: 795  GHRTESADRKADDTTSGIKHVSEKNPIETHTNISQESCISNSAEVSRQEEQYLPAVDSSP 854

Query: 2401 ------KERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSK 2240
                  KE    S+   + G  +PKL F L GKQLD SMT+YQA+L+++   + D++V  
Sbjct: 855  KQTMSAKEVTEGSSASPSIGSAKPKLTFSLRGKQLDSSMTIYQAVLEEQKGAEFDMVVGS 914

Query: 2239 KFWNEVHRVTLWSAKKLQLIYPQISADPRQSTLSWDKTELPWHNNFSFFSSMLLTELPFK 2060
            KFW+EV+++T  SA++ +    ++     QS++ W+K     H    +    LL ELP K
Sbjct: 915  KFWSEVYKLTYKSAEEPKANDSEMLNCVPQSSVFWNK-----HGFSDWKYPFLLAELPCK 969

Query: 2059 LERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAHSIPQTEFI 1880
            +++ N  YD+LF+LKI EG+N   FQL S+ER N+FAEG +ENFDDLKVI  SIPQ EF+
Sbjct: 970  IDKLNALYDVLFMLKIFEGMNHYLFQLLSDERLNSFAEGRIENFDDLKVIVSSIPQVEFV 1029

Query: 1879 NSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQT- 1703
            NSKL  KLEQQM D L L+T  MP WC QL+ ACPFLFSFEAR KYF L  FG  R+Q  
Sbjct: 1030 NSKLNDKLEQQMQDPLVLTTGCMPSWCGQLMAACPFLFSFEARRKYFYLTTFGSLRSQQN 1089

Query: 1702 --QNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNE 1529
              QN + +  +++NDR S S SL RKKF V+RNNIL SA KMM+ +A  K  LE+E++ E
Sbjct: 1090 NIQNLDGSGTNSLNDRHSYSGSL-RKKFIVNRNNILESAVKMMKLHAQSKGTLEVEYAEE 1148

Query: 1528 VGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFIIVHSGLFPRP 1349
            VGTGLGPT+EF+TL SH FQKVG+GMWRGD +      G S +   S F++   GLFPRP
Sbjct: 1149 VGTGLGPTMEFFTLASHEFQKVGLGMWRGDLSYA----GRSTIDGYSEFVLAPFGLFPRP 1204

Query: 1348 WSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEKDLDIYDIQ 1169
            WS   D S    F +V+K F LLG+L A+AI+DGRILD+PFSRAFYK++LE++L I DIQ
Sbjct: 1205 WSTSTDVSGVAEFPEVIKMFLLLGKLVAKAIKDGRILDIPFSRAFYKIILEQELSICDIQ 1264

Query: 1168 SFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEELCLDFSLPGYPDYV 989
            S DPELGRTM+EFQAL  RK FLES SG+ S    +  + NT +++LCLDF+LPG+PDY 
Sbjct: 1265 SIDPELGRTMLEFQALVNRKRFLESISGDSS----NLCYRNTSVKDLCLDFTLPGFPDYA 1320

Query: 988  FSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELE 809
              S   +KMVNI NLEEYV++VVDAT+ SGISRQ+++FKSGF++VF +KALQIFT +ELE
Sbjct: 1321 LLS-ESTKMVNIVNLEEYVTMVVDATIGSGISRQIDAFKSGFNEVFSLKALQIFTKDELE 1379

Query: 808  RLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQRRAFLLFVTGAPRL 629
            RLLCGEQD WD  EL +HINFDHGY+ +S   + FLEIIQE +  QRRAFL FVTG+PRL
Sbjct: 1380 RLLCGEQDCWDFTELVDHINFDHGYTGSSPTVVSFLEIIQELERDQRRAFLQFVTGSPRL 1439

Query: 628  PPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQ 449
            PPGGLAALKPKLT+V KH   DADM+LPSVMTCANYLKLPPYSSKE+MR KLLYAI EGQ
Sbjct: 1440 PPGGLAALKPKLTVVRKHSSCDADMDLPSVMTCANYLKLPPYSSKEKMRHKLLYAITEGQ 1499

Query: 448  GAFHLS 431
            G+FHLS
Sbjct: 1500 GSFHLS 1505


>gb|PNS98590.1| hypothetical protein POPTR_016G085200v3 [Populus trichocarpa]
          Length = 1504

 Score = 1217 bits (3150), Expect = 0.0
 Identities = 676/1399 (48%), Positives = 909/1399 (64%), Gaps = 21/1399 (1%)
 Frame = -1

Query: 4564 DPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVMLMSARVLTY 4385
            +PS Q+A LT  C+ L+F    D L+    D + P+LV L +    +PD+ML++ R LTY
Sbjct: 117  EPSGQLAALTELCEVLSFCT-EDSLSSTMADLLSPVLVRL-SRHDSNPDIMLLAIRALTY 174

Query: 4384 FLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVPCLRAGVIN 4205
              D+ PRA+   L++H A+ A+C  LM + YLDVAEQCL+AL+K++R Q +PCL+AG I 
Sbjct: 175  LCDVFPRASV-FLVRHDAIPAICQRLMAIEYLDVAEQCLQALEKITRDQPLPCLQAGAIM 233

Query: 4204 LVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAM 4025
             VLSF+DFF  S QR+ALSTV+N+CK++P + FS  M+AVP+LCNLL Y+DR+LVE+ A+
Sbjct: 234  AVLSFIDFFSTSVQRVALSTVVNICKKLPSENFSPFMEAVPILCNLLQYEDRQLVENVAI 293

Query: 4024 CLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSL 3845
            CL++I E    SSE+LDELCKHG++ Q+  L+  +S   LS+  Y G+I LLV+L++ S+
Sbjct: 294  CLIKIAERVSQSSEMLDELCKHGLINQATHLVQLNSRTTLSQPVYNGLIGLLVKLSSGSI 353

Query: 3844 LSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQ 3665
            ++ R+LYE NI+SIL+ +    D S      H  D   +QV+  LKLLN+L+P VAR+  
Sbjct: 354  VAFRTLYELNISSILKDLFATYDLSHGISSPHVIDGQGNQVHEVLKLLNELLPTVARNQD 413

Query: 3664 DIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKNYVLIATPD 3485
              QLVL KE  + + P L  +F   I+P+L Q V SGAN  + Y C+ VI   V ++  D
Sbjct: 414  AQQLVLDKEAFLANHPDLLHKFGSDIIPSLIQVVNSGANLYVCYGCLYVINKLVYLSKSD 473

Query: 3484 MLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRV 3305
            ML  L+KN N   FLAG+L+RKDHHVL+ AL + E I++KLP   +NSF+KEGV  AI  
Sbjct: 474  MLLELLKNTNFSSFLAGVLTRKDHHVLMLALQITETILQKLPDVFVNSFIKEGVFFAIDG 533

Query: 3304 LSKQDGCYQSQLMDQQSSN----LGDTDERTAANLSRCLCYAFDXXXXXXXXSK-MCNLP 3140
            L   + C  SQL+    +     L    + ++  + RCLCYAFD            C L 
Sbjct: 534  LLVPEKC--SQLIFPACNGIHLPLNSNQKSSSKVVMRCLCYAFDTGQSLSASETGTCKLE 591

Query: 3139 RHTLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDK--NVDSALTNCAEREDY 2969
            + T+  L + +  ++F             + QKL+A    L    N+   + +C + E+ 
Sbjct: 592  KDTVENLGKHIRISYFALESCDSEKGLTDILQKLRALSAELSDLMNMSVKIGSCTQDEEK 651

Query: 2968 LSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGDMSFD-YLTVLK 2792
               ++ + +  L+  + +STFEFIESG ++ L +YL NG YL   ++   +FD +  V K
Sbjct: 652  CYSILCQIMEKLDGREPVSTFEFIESGIVKILVNYLFNGKYLREKVEPQSTFDDFYVVEK 711

Query: 2791 RLQTFSFKLLSGHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSST 2612
            R + F+  L S    +  PL++L+Q LQ ALS+ E FPV+LS A+K +S    IP    T
Sbjct: 712  RFEVFARLLSSSDLSEESPLSALIQKLQGALSSSENFPVILSHASKYRSSFAIIPNGRRT 771

Query: 2611 KHPCLQVHFVTEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXTQ- 2438
             +PCL+V FV  EGET LC Y ++ +TVD  SS + IE FL P                 
Sbjct: 772  SYPCLRVRFVRGEGETCLCNYSEDPVTVDPLSSVNTIEGFLSPKVRIKGTEQIESAAQAL 831

Query: 2437 --------RHIKQSSRKVDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAIL 2282
                      I QS    D   +    T+ +N GD  PKL+F L+G+QLDR++TLYQAIL
Sbjct: 832  EPAENVSVEDIVQSPSCADDSTKSHCPTSCSN-GDAMPKLVFYLEGQQLDRTLTLYQAIL 890

Query: 2281 QDKINEDPDIIVSKKFWNEVHRVTLWSAKKLQLIYPQISADPRQSTLSWDKTELPWHNNF 2102
            Q K+  D +I  + K W +VH +T   A   +    Q      Q++   D+  + +  + 
Sbjct: 891  QQKVKADHEINSTAKLWTQVHTLTYRIAVDTRDDNTQDCPSMAQNSSILDQA-VAFMQHP 949

Query: 2101 SFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDD 1922
            +FFSSM   ELP  L++S+P  D+LF+LK LEGLNR  F L S+ER +AFAEG ++N D+
Sbjct: 950  AFFSSMFNCELPSDLDKSSPTNDILFLLKSLEGLNRFIFHLMSHERIHAFAEGLIDNLDN 1009

Query: 1921 LKVIAHSIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKY 1742
            L+V A  + Q EF++SKLT KLEQQM D+LA+S   MP+WC QL+ +C FLFSFE R KY
Sbjct: 1010 LRVAARPVAQNEFVSSKLTEKLEQQMRDSLAVSMGGMPVWCNQLMNSCSFLFSFETRCKY 1069

Query: 1741 FCLNIFGVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGK 1562
            F L+ FG  + Q Q S  N    + DR   + SL RKKF V R+ +L SAA+MM  YA  
Sbjct: 1070 FQLSAFGCQQIQIQPSSHNNSGVLRDRLPSAGSLSRKKFIVLRDQVLESAAQMMDRYAHL 1129

Query: 1561 KVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTS--TAKRLGTSMVVSDS 1388
            KV +E+ ++ EVGTGLGPTLEFYTLVS  FQK G+GMWR DH S  T + L        S
Sbjct: 1130 KVPIEVVYNEEVGTGLGPTLEFYTLVSKEFQKSGIGMWREDHISFPTIENLQAEY----S 1185

Query: 1387 GFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYK 1208
            G +    GLFPRPWS  +D S+GV FS+V+K FFLLGQ+ A+A+QDGR+LDLPF++ FYK
Sbjct: 1186 GIVKSPFGLFPRPWSPTVDASDGVQFSEVIKKFFLLGQIVAKALQDGRVLDLPFAKVFYK 1245

Query: 1207 LMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEEL 1028
            L+L+++L++YDIQSFDPELGRT++EFQAL  RK  +     E+S    D  F NTRIE+L
Sbjct: 1246 LILQQELNLYDIQSFDPELGRTLLEFQALVNRKKNMGLVIVENSSSTQDACFWNTRIEDL 1305

Query: 1027 CLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFP 848
            CLDF+LPGY DY+ S   D K+VN+ NLE YVS +VDAT+ +GISRQ+E+FKSGF++VFP
Sbjct: 1306 CLDFTLPGYSDYILSFDEDHKIVNMDNLEVYVSHIVDATIHTGISRQVEAFKSGFNQVFP 1365

Query: 847  IKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQR 668
            IK L IFT EELERLLCGE+D W   EL +HI FDHGY+++S P +  LEII+EF+  QR
Sbjct: 1366 IKHLMIFTEEELERLLCGERDFWAFNELLDHIKFDHGYTASSPPIVNLLEIIKEFEYEQR 1425

Query: 667  RAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKER 488
            R+FL FVTGAPRLP GGLA+L PKLTIV KHC N  D++LPSVMTCANYLKLPPYSSK++
Sbjct: 1426 RSFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNCEDVDLPSVMTCANYLKLPPYSSKDK 1485

Query: 487  MREKLLYAIREGQGAFHLS 431
            M+EKLLYAI EGQG+FHLS
Sbjct: 1486 MKEKLLYAITEGQGSFHLS 1504


>gb|PNS98589.1| hypothetical protein POPTR_016G085200v3 [Populus trichocarpa]
          Length = 1512

 Score = 1195 bits (3091), Expect = 0.0
 Identities = 674/1412 (47%), Positives = 904/1412 (64%), Gaps = 34/1412 (2%)
 Frame = -1

Query: 4564 DPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVMLMSARVLTY 4385
            +PS Q+A LT  C+ L+F    D L+    D + P+LV L +    +PD+ML++ R LTY
Sbjct: 117  EPSGQLAALTELCEVLSFCT-EDSLSSTMADLLSPVLVRL-SRHDSNPDIMLLAIRALTY 174

Query: 4384 FLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVPCLRAGVIN 4205
              D+ PRA+   L++H A+ A+C  LM + YLDVAEQCL+AL+K++R Q +PCL+AG I 
Sbjct: 175  LCDVFPRASV-FLVRHDAIPAICQRLMAIEYLDVAEQCLQALEKITRDQPLPCLQAGAIM 233

Query: 4204 LVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAM 4025
             VLSF+DFF  S QR+ALSTV+N+CK++P + FS  M+AVP+LCNLL Y+DR+LVE+ A+
Sbjct: 234  AVLSFIDFFSTSVQRVALSTVVNICKKLPSENFSPFMEAVPILCNLLQYEDRQLVENVAI 293

Query: 4024 CLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSL 3845
            CL++I E    SSE+LDELCKHG++ Q+  L+  +S   LS+  Y G+I LLV+L++ S+
Sbjct: 294  CLIKIAERVSQSSEMLDELCKHGLINQATHLVQLNSRTTLSQPVYNGLIGLLVKLSSGSI 353

Query: 3844 LSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQ 3665
            ++ R+LYE NI+SIL+ +    D S      H  D   +QV+  LKLLN+L+P VAR+  
Sbjct: 354  VAFRTLYELNISSILKDLFATYDLSHGISSPHVIDGQGNQVHEVLKLLNELLPTVARNQD 413

Query: 3664 DIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKNYVLIATPD 3485
              QLVL KE  + + P L  +F   I+P+L Q V SGAN  + Y C+ VI   V ++  D
Sbjct: 414  AQQLVLDKEAFLANHPDLLHKFGSDIIPSLIQVVNSGANLYVCYGCLYVINKLVYLSKSD 473

Query: 3484 MLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRV 3305
            ML  L+KN N   FLAG+L+RKDHHVL+ AL + E I++KLP   +NSF+KEGV  AI  
Sbjct: 474  MLLELLKNTNFSSFLAGVLTRKDHHVLMLALQITETILQKLPDVFVNSFIKEGVFFAIDG 533

Query: 3304 LSKQDGCYQSQLMDQQSSN----LGDTDERTAANLSRCLCYAFDXXXXXXXXSK-MCNLP 3140
            L   + C  SQL+    +     L    + ++  + RCLCYAFD            C L 
Sbjct: 534  LLVPEKC--SQLIFPACNGIHLPLNSNQKSSSKVVMRCLCYAFDTGQSLSASETGTCKLE 591

Query: 3139 RHTLLPLAQRV-MSTFFXXXXXXXXXXXXXLQKLKACYIALD--KNVDSALTNCAEREDY 2969
            + T+  L + + +S F              LQKL+A    L    N+   + +C + E+ 
Sbjct: 592  KDTVENLGKHIRISYFALESCDSEKGLTDILQKLRALSAELSDLMNMSVKIGSCTQDEEK 651

Query: 2968 LSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGDMSF-DYLTVLK 2792
               ++ + +  L+  + +STFEFIESG ++ L +YL NG YL   ++   +F D+  V K
Sbjct: 652  CYSILCQIMEKLDGREPVSTFEFIESGIVKILVNYLFNGKYLREKVEPQSTFDDFYVVEK 711

Query: 2791 RLQTFSFKLLSGHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSST 2612
            R + F+  L S    +  PL++L+Q LQ ALS+ E FPV+LS A+K +S    IP    T
Sbjct: 712  RFEVFARLLSSSDLSEESPLSALIQKLQGALSSSENFPVILSHASKYRSSFAIIPNGRRT 771

Query: 2611 KHPCLQVHFVTEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXTQ- 2438
             +PCL+V FV  EGET LC Y ++ +TVD  SS + IE FL P                 
Sbjct: 772  SYPCLRVRFVRGEGETCLCNYSEDPVTVDPLSSVNTIEGFLSPKVRIKGTEQIESAAQAL 831

Query: 2437 --------RHIKQSSRKVDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAIL 2282
                      I QS    D   +    T+ +N GD  PKL+F L+G+QLDR++TLYQAIL
Sbjct: 832  EPAENVSVEDIVQSPSCADDSTKSHCPTSCSN-GDAMPKLVFYLEGQQLDRTLTLYQAIL 890

Query: 2281 QDKINEDPDIIVSKKFWNEVHRVTLWSAKKLQLIYPQISADPRQSTLSWDKTELPWHNNF 2102
            Q K+  D +I  + K W +VH +T   A   +    Q      Q++   D+  + +  + 
Sbjct: 891  QQKVKADHEINSTAKLWTQVHTLTYRIAVDTRDDNTQDCPSMAQNSSILDQA-VAFMQHP 949

Query: 2101 SFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDD 1922
            +FFSSM   ELP  L++S+P  D+LF+LK LEGLNR  F L S+ER +AFAEG ++N D+
Sbjct: 950  AFFSSMFNCELPSDLDKSSPTNDILFLLKSLEGLNRFIFHLMSHERIHAFAEGLIDNLDN 1009

Query: 1921 LKVIAHSIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKY 1742
            L+V A  + Q EF++SKLT KLEQQM D+LA+S   MP+WC QL+ +C FLFSFE R KY
Sbjct: 1010 LRVAARPVAQNEFVSSKLTEKLEQQMRDSLAVSMGGMPVWCNQLMNSCSFLFSFETRCKY 1069

Query: 1741 FCLNIFGVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGK 1562
            F L+ FG  + Q Q S  N    + DR   + SL RKKF V R+ +L SAA+MM  YA  
Sbjct: 1070 FQLSAFGCQQIQIQPSSHNNSGVLRDRLPSAGSLSRKKFIVLRDQVLESAAQMMDRYAHL 1129

Query: 1561 KVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTS--TAKRLGTSMVVSDS 1388
            KV +E+ ++ EVGTGLGPTLEFYTLVS  FQK G+GMWR DH S  T + L        S
Sbjct: 1130 KVPIEVVYNEEVGTGLGPTLEFYTLVSKEFQKSGIGMWREDHISFPTIENLQAEY----S 1185

Query: 1387 GFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYK 1208
            G +    GLFPRPWS  +D S+GV FS+V+K FFLLGQ+ A+A+QDGR+LDLPF++ FYK
Sbjct: 1186 GIVKSPFGLFPRPWSPTVDASDGVQFSEVIKKFFLLGQIVAKALQDGRVLDLPFAKVFYK 1245

Query: 1207 LMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEEL 1028
            L+L+++L++YDIQSFDPELGRT++EFQAL  RK  +     E+S    D  F NTRIE+L
Sbjct: 1246 LILQQELNLYDIQSFDPELGRTLLEFQALVNRKKNMGLVIVENSSSTQDACFWNTRIEDL 1305

Query: 1027 CLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFP 848
            CLDF+LPGY DY+ S   D K+VN+ NLE YVS +VDAT+ +GISRQ+E+FKSGF++VFP
Sbjct: 1306 CLDFTLPGYSDYILSFDEDHKIVNMDNLEVYVSHIVDATIHTGISRQVEAFKSGFNQVFP 1365

Query: 847  IKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQR 668
            IK L IFT EELERLLCGE+D W   EL +HI FDHGY+++S P +       EF+  QR
Sbjct: 1366 IKHLMIFTEEELERLLCGERDFWAFNELLDHIKFDHGYTASSPPIVNV-----EFEYEQR 1420

Query: 667  RAFLLFVTGAPRLPPGGLAALKPKLTIVCK-------------HCDNDADMELPSVMTCA 527
            R+FL FVTGAPRLP GGLA+L PKLTIV K             HC N  D++LPSVMTCA
Sbjct: 1421 RSFLQFVTGAPRLPTGGLASLNPKLTIVRKSLFNHLSSWCSLQHCSNCEDVDLPSVMTCA 1480

Query: 526  NYLKLPPYSSKERMREKLLYAIREGQGAFHLS 431
            NYLKLPPYSSK++M+EKLLYAI EGQG+FHLS
Sbjct: 1481 NYLKLPPYSSKDKMKEKLLYAITEGQGSFHLS 1512


>ref|XP_022939346.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita moschata]
          Length = 1485

 Score = 1096 bits (2834), Expect = 0.0
 Identities = 625/1395 (44%), Positives = 878/1395 (62%), Gaps = 19/1395 (1%)
 Frame = -1

Query: 4558 SAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVMLMSARVLTYFL 4379
            SAQ   L   C+ L+F    + L+ +  D +  +LV L+      P+++L++ R +TY  
Sbjct: 120  SAQRTLLVELCEVLSFCM-ENSLSSMTSDSLSIVLVNLVK-LDSDPEIVLLALRAITYLC 177

Query: 4378 DLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVPCLRAGVINLV 4199
            D+ PR++   L++H  V ALC  L  + YLDVAEQCL AL+K+SR Q V CL AG +  V
Sbjct: 178  DVYPRSSS-FLVRHDGVRALCQMLGVIKYLDVAEQCLIALEKISREQPVACLEAGAVMAV 236

Query: 4198 LSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCL 4019
            L+++DFF  S QR AL TV+NVC+++P +C   ++DAVP+LCNLL Y+D +L E+ A+C+
Sbjct: 237  LTYIDFFSTSIQRTALRTVVNVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCM 296

Query: 4018 MRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLS 3839
            ++IVE    S ELLD LC+HG+++Q + LI+ +S   LS+TTY  ++ +LV+L++ S ++
Sbjct: 297  IKIVERVSQSCELLDGLCRHGLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVA 356

Query: 3838 IRSLYESNINSILESILR--NPDHSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQ 3665
             ++LYE NI++ L+ IL   N  H   +  S   D  R+QV   LKLLN+L+P     D 
Sbjct: 357  FKTLYELNISNTLKDILSAYNLSHGMSSSCS-VVDGQRNQVCEVLKLLNELLPT---GDA 412

Query: 3664 DIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKNYVLIATPD 3485
            + + +  K   ++  PK   +F + +LP L Q V+SGAN  +   C+++I  +V +   D
Sbjct: 413  NAEELSEKVSFLVSNPKKLQKFGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESD 472

Query: 3484 MLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRV 3305
            ML  L++N N+  FLAG+ +RKDHHVL+ AL + EII++KL      SFVKEGV  AI  
Sbjct: 473  MLVELLQNTNISSFLAGVFTRKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDA 532

Query: 3304 LSKQDGCYQS--QLMDQQSSNLGDTDERTAANLSRCLCYAF-DXXXXXXXXSKMCNLPRH 3134
            L   +   Q    +      ++G   + +  N +RCLCYAF          +  C L + 
Sbjct: 533  LITPEKYKQLIFPVFTGVHPSIGSCQKSSREN-ARCLCYAFLSGCFSSTEETGNCKLDKD 591

Query: 3133 TLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTNC--AEREDYLS 2963
            ++  LA  + + +F             + Q L+    +LD  ++ +L     A+ E+   
Sbjct: 592  SVYSLAYHIRTHYFSEELCDTDKGVTDILQNLQTLSGSLDDLLNLSLIKDTPAQDEENFY 651

Query: 2962 QMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGDMSFDYLTVL-KRL 2786
             ++ E ++ L  G+ ISTFEFIESG ++   +YLTNG Y+ +  +  +     +++ +R 
Sbjct: 652  ALLAEIMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYMRKKGEPHIISRQFSIMERRF 711

Query: 2785 QTFSFKLLSGHS--GQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSST 2612
            + F+  LLS      +  P+  L++ LQ +LS+LE FPVV S   K ++    +P     
Sbjct: 712  EVFARLLLSSAEPPSESLPVLVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCI 771

Query: 2611 KHPCLQVHFVTEEGETDLCLYD-NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXTQR 2435
             HPC++V FV  +GETDLC Y  ++ TVD  SS +AIE FLW               + R
Sbjct: 772  PHPCVKVRFVRGDGETDLCNYTGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLR 831

Query: 2434 H-----IKQSSRKVDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAILQDKI 2270
                  +   S +V++ E++  S + + +G ++PKL+  L+GKQL+ ++TLYQAILQ  +
Sbjct: 832  EHQIKLLSNGSTEVNTDEKLQYSVSCSKKG-MKPKLLLYLEGKQLEPTLTLYQAILQQHM 890

Query: 2269 NEDPDIIVSKKFWNEVHRVTLWSAKKLQ--LIYPQISADPRQSTLSWDKTELPWHNNFSF 2096
             E+ + I   K WN+V+ +T  SA + +       +SA  + +TL            FS 
Sbjct: 891  KEN-ETISGTKLWNKVYTLTYKSAGESEDNSSNELLSASDKAATLK-----------FSS 938

Query: 2095 FSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLK 1916
            F   L   LP  L + +PAYD+LF+L+ +EG+NR++F + S+ER  AFAEG +   D++K
Sbjct: 939  FFHGLDCILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDNIK 998

Query: 1915 VIAHSIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKYFC 1736
            +   S+PQ EF+NSKLT KLEQQM D  A S   MPLWC +L+ +CPFLFSF+AR KYF 
Sbjct: 999  LSFPSVPQNEFVNSKLTEKLEQQMRDFSAFSIGGMPLWCKELMDSCPFLFSFDARCKYFR 1058

Query: 1735 LNIFGVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKV 1556
            +  F +S  Q      +++ T ND RS S  LPRKK  VHRN IL SA KMM  YA +KV
Sbjct: 1059 IVAFAMSHRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQKV 1118

Query: 1555 LLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFII 1376
            LLE+E+  EVGTGLGPTLEFYTLV   FQK  +GMWRGDH +     G   V S  G   
Sbjct: 1119 LLEVEYDEEVGTGLGPTLEFYTLVGREFQKFDLGMWRGDHGALISIEGRETVESPFG--- 1175

Query: 1375 VHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLE 1196
                LFPRPW + +D ++ + FS+V+K F LLG++ ARAIQDGR++D+ FS+AFYKL+L 
Sbjct: 1176 ----LFPRPWPSTLD-THELYFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYKLILG 1230

Query: 1195 KDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEELCLDF 1016
            +++ IYDIQSFDPELG  ++EFQAL  R   LES   E+S  + +  +HNT IE+LCLDF
Sbjct: 1231 QEVSIYDIQSFDPELGTALLEFQALVDRGKLLESVCEENSSAKLEFCYHNTNIEDLCLDF 1290

Query: 1015 SLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKAL 836
            ++PGYPDY+  S  D  MVN  NLE+YVSLVVDAT+ SGISRQ+E+FKSGF++VFPI+ L
Sbjct: 1291 TVPGYPDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGISRQIEAFKSGFNQVFPIEHL 1350

Query: 835  QIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQRRAFL 656
            Q+FTAEELERL+CGE D+W + EL  ++ FDHGY+++S   +  LEIIQEFD   +RAFL
Sbjct: 1351 QVFTAEELERLICGEHDSWALNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQRAFL 1410

Query: 655  LFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREK 476
             FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMTCANYLKLPPYSSKE M+EK
Sbjct: 1411 QFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEIMKEK 1470

Query: 475  LLYAIREGQGAFHLS 431
            LLYAI EGQG+FHLS
Sbjct: 1471 LLYAITEGQGSFHLS 1485


>ref|XP_023005685.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita maxima]
          Length = 1486

 Score = 1091 bits (2821), Expect = 0.0
 Identities = 624/1395 (44%), Positives = 871/1395 (62%), Gaps = 19/1395 (1%)
 Frame = -1

Query: 4558 SAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVMLMSARVLTYFL 4379
            SAQ   L   C+ L+F    + L+ +  D I  +LV L+      P+++L++ R +TY  
Sbjct: 121  SAQRTLLVELCEVLSFCM-ENSLSSMTSDSISIVLVNLVK-LDSDPEIVLLALRAITYLC 178

Query: 4378 DLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVPCLRAGVINLV 4199
            D+ PR++   L++H  V ALC  L  + YLDVAEQCL AL+K+S+ Q V CL AG +  V
Sbjct: 179  DVYPRSSS-FLVRHDGVRALCQMLGVIKYLDVAEQCLIALEKISQEQPVACLEAGAVMAV 237

Query: 4198 LSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCL 4019
            L+++DFF  S QR AL TV+NVC+++P +C   ++DAVP+LCNLL Y+D +L E+ A+C+
Sbjct: 238  LTYIDFFSTSIQRTALRTVVNVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCM 297

Query: 4018 MRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLS 3839
            ++IVE    S ELLD LC HG+++Q + LI+ +S   LS+TTY  ++ +LV+L++ S ++
Sbjct: 298  IKIVERVSQSCELLDGLCHHGLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVA 357

Query: 3838 IRSLYESNINSILESILR--NPDHSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQ 3665
             ++LYE NI++ L+ IL   N  H   +  S   D  R+QV   LKLLN+L+P     D 
Sbjct: 358  FKTLYELNISNTLKDILSAYNLSHGMSSSCS-VVDGQRNQVCEVLKLLNELLPT---GDA 413

Query: 3664 DIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKNYVLIATPD 3485
            + + +  K   ++  PK   +F + +LP L Q V+SGAN      C+++I  +V +    
Sbjct: 414  NAEELSEKVSFLVSNPKKLQKFGLDVLPLLVQVVSSGANLYACCGCLTIIYKFVCLGESG 473

Query: 3484 MLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRV 3305
            ML  L++N N+  FLAG+ +RKDHHVL+ AL + EII++KL      SFVKEGV  AI  
Sbjct: 474  MLVELLQNTNISSFLAGVFTRKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDA 533

Query: 3304 LSKQDGCYQS--QLMDQQSSNLGDTDERTAANLSRCLCYAF-DXXXXXXXXSKMCNLPRH 3134
            L   +   Q    +      + G   + +  N +RCLCYAF          +  C L + 
Sbjct: 534  LITPEKYKQLIFPVFTGVHPSFGSCQKSSREN-ARCLCYAFLSGCFSSTEETGNCKLDKD 592

Query: 3133 TLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTNC--AEREDYLS 2963
            ++  LA  + + +F             + Q L+    +LD  ++ +L     A+ E+   
Sbjct: 593  SVYSLAYHIRTHYFSEELCDTDKGVTDILQNLQTLSGSLDDLLNLSLIKDTPAQDEENFY 652

Query: 2962 QMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGDM-SFDYLTVLKRL 2786
             ++ E ++ L  G+ ISTFEFIESG ++   +YLTNG Y+ +  +  + S  +  + +R 
Sbjct: 653  ALLAEIMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYMRKKGEPQIISRQFAIMERRF 712

Query: 2785 QTFSFKLLSGHS--GQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSST 2612
            + F+  LLS      +  P+  L++ LQ +LS+LE FPVV S   K ++    +P     
Sbjct: 713  EVFARLLLSSSEPPSESLPVLVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCI 772

Query: 2611 KHPCLQVHFVTEEGETDLCLYD-NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXTQR 2435
             HPC++V FV  +GETDLC Y  ++ TVD  SS +AIE FLWP              + R
Sbjct: 773  PHPCVKVRFVRGDGETDLCNYTGDIHTVDPFSSLNAIEGFLWPKVGTKKAEQSFEADSLR 832

Query: 2434 H-----IKQSSRKVDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAILQDKI 2270
                  +   S +V++ E++  S + + +G ++PKL+  L+GKQL+ ++TLYQAILQ  +
Sbjct: 833  EHQIKLLSNGSTEVNTDEKLQYSASCSKKG-MKPKLLLYLEGKQLEPTLTLYQAILQQHM 891

Query: 2269 NEDPDIIVSKKFWNEVHRVTLWSAKKLQ--LIYPQISADPRQSTLSWDKTELPWHNNFSF 2096
             E+ +     K WN+V+ +T  SA + +        SA  + +TL            FS 
Sbjct: 892  KEN-ETTSGTKLWNQVYTLTYKSAGEREDNSSNKLFSASDKAATLQ-----------FSS 939

Query: 2095 FSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLK 1916
            F   L   LP  L + +PAYD+LF+L+ +EG+NR++F + S+ER  AFAEG +   D++K
Sbjct: 940  FFHSLDCILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDNIK 999

Query: 1915 VIAHSIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKYFC 1736
            +   S+PQ EF+NSKLT KLEQQM D  A S   MPLWC +L+ +CPFLFSF+AR KYF 
Sbjct: 1000 LSVTSVPQNEFVNSKLTEKLEQQMRDFSAFSVGGMPLWCKELMDSCPFLFSFDARCKYFQ 1059

Query: 1735 LNIFGVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKV 1556
            +  F +S  Q      +++ T ND RS S  LPRKK  VHRN IL SA KMM  YA +KV
Sbjct: 1060 IVAFAMSHRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQKV 1119

Query: 1555 LLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFII 1376
            LLE+E+  EVGTGLGPTLEFYTLV   FQK  +GMWRGDH +     G   V S  G   
Sbjct: 1120 LLEVEYDEEVGTGLGPTLEFYTLVGREFQKYDLGMWRGDHGALISLEGRETVESPFG--- 1176

Query: 1375 VHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLE 1196
                LFPRPW + +D +  + FS+V+K F LLG++ ARAIQDGR++D+ FS+AFYKL+L 
Sbjct: 1177 ----LFPRPWPSTLD-TRELYFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYKLILG 1231

Query: 1195 KDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEELCLDF 1016
            +++ IYDIQSFDPELG  ++EFQAL  R   LES   E+S  + +  +HNT IE+LCLDF
Sbjct: 1232 QEVSIYDIQSFDPELGTALLEFQALVDRGKLLESVCEENSSAKLEFCYHNTNIEDLCLDF 1291

Query: 1015 SLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKAL 836
            ++PGYPDY+  S  D  MVN  NLE+YVSLVVDAT+ SGISRQ+E+FKSGF++VFPI+ L
Sbjct: 1292 TVPGYPDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGISRQIEAFKSGFNQVFPIEHL 1351

Query: 835  QIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQRRAFL 656
            Q+FT EELERL+CGE D+W + EL  ++ FDHGY+++S   +  LEIIQEFD   +RAFL
Sbjct: 1352 QVFTEEELERLICGEHDSWALNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQRAFL 1411

Query: 655  LFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREK 476
             FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMTCANYLKLPPYSSKE M+EK
Sbjct: 1412 QFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEIMKEK 1471

Query: 475  LLYAIREGQGAFHLS 431
            LLYAI EGQG+FHLS
Sbjct: 1472 LLYAITEGQGSFHLS 1486


>ref|XP_023539461.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita pepo
            subsp. pepo]
          Length = 1485

 Score = 1089 bits (2817), Expect = 0.0
 Identities = 622/1395 (44%), Positives = 873/1395 (62%), Gaps = 19/1395 (1%)
 Frame = -1

Query: 4558 SAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVMLMSARVLTYFL 4379
            SAQ   L   C+ L+F    + L+ +  D +  +LV L+      P+++L++ R +TY  
Sbjct: 120  SAQRTLLVELCEVLSFCM-ENSLSSMTSDSLSIVLVNLVK-LDSDPEIVLLALRAITYLC 177

Query: 4378 DLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVPCLRAGVINLV 4199
            D+ PR++   L++H  V ALC  L  + YLDVAEQCL AL+K+SR Q V CL AG +  V
Sbjct: 178  DVYPRSSS-FLVRHDGVRALCQMLGVIKYLDVAEQCLIALEKISREQPVACLEAGAVMAV 236

Query: 4198 LSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCL 4019
            L+++DFF  S QR AL TV+NVC+++P +C   ++DAVP+LCNLL Y+D +L E+ A+C+
Sbjct: 237  LTYIDFFSTSIQRTALRTVVNVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCM 296

Query: 4018 MRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLS 3839
            ++IVE    S ELLD LC HG+++Q + LI+ +S   LS+TTY  ++ +LV+L++ S ++
Sbjct: 297  IKIVERVSQSCELLDGLCHHGLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVA 356

Query: 3838 IRSLYESNINSILESILR--NPDHSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQ 3665
             ++LYE NI++ L+ IL   N  H   +  S   D  R+QV   LKLLN+L+P     D 
Sbjct: 357  FKTLYELNISNTLKDILSAYNLSHGMSSSCS-VVDGQRNQVCEVLKLLNELLPT---GDA 412

Query: 3664 DIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKNYVLIATPD 3485
            + + +  K   ++  PK   +F + +LP L Q V+SGAN  +   C+++I  +V +    
Sbjct: 413  NAEELSEKVYFLVSNPKKLQKFGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESG 472

Query: 3484 MLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRV 3305
            ML  L++N N+  FLAG+ +RKDHHVL+ AL + EII++KL      SFVKEGV  AI  
Sbjct: 473  MLVELLQNTNISSFLAGVFTRKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDA 532

Query: 3304 LSKQDGCYQS--QLMDQQSSNLGDTDERTAANLSRCLCYAF-DXXXXXXXXSKMCNLPRH 3134
            L   +   Q    +      ++G   + +  N +RCLCYAF          +  C L + 
Sbjct: 533  LITPEKYKQLIFPVFTGVHPSIGSCQKSSREN-ARCLCYAFLSGCFSSTEETGNCKLDKD 591

Query: 3133 TLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTN--CAEREDYLS 2963
            ++  LA  + + +F             + Q L+    +LD  ++ +L     A+ E+   
Sbjct: 592  SVYSLAYHIRTHYFSEELCDTDKGVTDILQNLQTLAGSLDDLLNLSLIKETPAQDEENCY 651

Query: 2962 QMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGDMSFDYLTVL-KRL 2786
             ++ E ++ L  G+ ISTFEFIESG ++   +YLTNG Y+ +  +  +     +++ +R 
Sbjct: 652  ALLAEIMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYMRKKGEPQIISRQFSIMERRF 711

Query: 2785 QTFSFKLLSGHS--GQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSST 2612
            + F+  LLS      +  P+  L++ LQ +LS+LE FPVV S   K ++    +P     
Sbjct: 712  EVFARLLLSSAEPPSESLPVLVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCI 771

Query: 2611 KHPCLQVHFVTEEGETDLCLYD-NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXTQR 2435
             HPC++V FV  +GETDLC Y  ++ TVD  SS +AIE FLW               + R
Sbjct: 772  PHPCVKVRFVRGDGETDLCNYTGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLR 831

Query: 2434 H-----IKQSSRKVDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAILQDKI 2270
                  +   S +VD+ E++  S + + +G ++PKL+  L+GKQL+ ++TLYQAILQ  +
Sbjct: 832  EHQIKLLSNGSTEVDTDEKLQYSVSCSKKG-MKPKLLLYLEGKQLEPTLTLYQAILQQHM 890

Query: 2269 NEDPDIIVSKKFWNEVHRVTLWSAKKLQ--LIYPQISADPRQSTLSWDKTELPWHNNFSF 2096
             E  + +   K WN+V+ +T  SA + +        SA  + +TL            FS 
Sbjct: 891  KES-ETVSGTKLWNQVYTLTYKSAGESEDNSSNELFSASDKAATLK-----------FSS 938

Query: 2095 FSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLK 1916
            F   L   LP  L + +PAYD+LF+L+ +EG+NR++F + S+ER  AFAEG +   D++K
Sbjct: 939  FFHGLDCILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDNIK 998

Query: 1915 VIAHSIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKYFC 1736
            +   S+PQ EF+NSKLT KLEQQM D  A S   MPLWC +L+ +CPFLFSF+AR KYF 
Sbjct: 999  LSVPSVPQNEFVNSKLTEKLEQQMRDFSAFSIGGMPLWCKELMDSCPFLFSFDARCKYFR 1058

Query: 1735 LNIFGVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKV 1556
            +  F +S  Q      +++ T ND RS S  LPRKK  VHRN IL SA KMM  YA +KV
Sbjct: 1059 IVAFSMSHRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQKV 1118

Query: 1555 LLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFII 1376
            LLE+E+  EVGTGLGPTLEFYTLV   FQK  +GMWRGDH +     G   V S  G   
Sbjct: 1119 LLEVEYDEEVGTGLGPTLEFYTLVGREFQKYDLGMWRGDHGALISIEGRETVESPFG--- 1175

Query: 1375 VHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLE 1196
                LFPRPW + +D ++ + FS+V+K F LLG++ ARAIQDGR++D+ FS+AFYKL+L 
Sbjct: 1176 ----LFPRPWPSTLD-THELYFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYKLILG 1230

Query: 1195 KDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEELCLDF 1016
            +++ IYDIQSFDPELG  ++EFQAL  R   LE    E+S  + +  +HNT IE+LCLDF
Sbjct: 1231 QEVSIYDIQSFDPELGTALLEFQALVDRGKLLEFVCEENSSAKLEFCYHNTNIEDLCLDF 1290

Query: 1015 SLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKAL 836
            ++PGYPDY+  S  D  MVN  NLE+YVSLVVDAT+ SGISRQ+++FKSGF++VFPI+ L
Sbjct: 1291 TVPGYPDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGISRQIDAFKSGFNQVFPIEHL 1350

Query: 835  QIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQRRAFL 656
            Q+FTAEELERL+CGE D+W + EL  ++ FDHGY+++S   +  LEIIQEFD   +RAFL
Sbjct: 1351 QVFTAEELERLICGEHDSWALNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQRAFL 1410

Query: 655  LFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREK 476
             FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMTCANYLKLPPYSSKE M+EK
Sbjct: 1411 QFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEIMKEK 1470

Query: 475  LLYAIREGQGAFHLS 431
            LLYAI EGQG+FHLS
Sbjct: 1471 LLYAITEGQGSFHLS 1485


>gb|PIA45462.1| hypothetical protein AQUCO_01700766v1, partial [Aquilegia coerulea]
          Length = 1426

 Score = 1087 bits (2810), Expect = 0.0
 Identities = 624/1391 (44%), Positives = 875/1391 (62%), Gaps = 15/1391 (1%)
 Frame = -1

Query: 4558 SAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVEL-LAGRGGSPDVMLMSARVLTYF 4382
            S+Q+  LT  CD L+F+      + L L   VP+LV + +     +PD+ML + R ++Y 
Sbjct: 90   SSQLVALTELCDTLSFSPEHSIRSKL-LQPFVPILVAMSIQETTMNPDIMLFAMRAISYA 148

Query: 4381 LDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVPCLRAGVINL 4202
             D  P +    L++HGAV +L  PL+ + YLDVAEQCL+ L+K+SR   +PCL AG I  
Sbjct: 149  CDASPSSVP-LLVQHGAVVSLVAPLLAIEYLDVAEQCLQGLEKISRRHPLPCLEAGTIMT 207

Query: 4201 VLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMC 4022
            +L+++DFF  S QR+ ++ + N+CK++P +    VM+AVP+LCNLL Y+D+KLVE+ A C
Sbjct: 208  ILNYIDFFASSVQRVGITIIANICKKLPSEFSPQVMEAVPLLCNLLYYEDQKLVENVATC 267

Query: 4021 LMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLL 3842
            L+ IV+   HS ++LDE+CK G+ +Q   LI+ +    L++  Y  +I LL RLA+ S+ 
Sbjct: 268  LIWIVKKASHSPDMLDEVCKQGLTQQVAHLIASNVGNILNQPIYTDLIGLLSRLASGSIT 327

Query: 3841 SIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQD 3662
            +  SL E NI++ +  ILR+ D S   PPS   ++        +KLL  L+PP++ +   
Sbjct: 328  ASNSLLEHNISNTMSLILRSYDLSHSKPPSLIHEV--------IKLLYVLLPPLSTNCNG 379

Query: 3661 IQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKNYVLIATPDM 3482
               V   ++II  +P L  QF   +LP L Q V SGAN  I Y C+SV+   +  +T DM
Sbjct: 380  KTEVSNMDRIIAHQPGLLQQFGADVLPVLVQIVNSGANTHICYGCLSVVNKIIHFSTSDM 439

Query: 3481 LRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVL 3302
            L  L+KNAN+  FLAG+++RK+ H+L+ AL +V+ +M++LP    NSF+KEGV + I  L
Sbjct: 440  LVDLLKNANISSFLAGIIARKELHLLMLALTIVKNVMQELPDAFFNSFIKEGVVYTIDAL 499

Query: 3301 SKQDGCYQSQLMDQQSSNL-GDTDERTAANLS-RCLCYAFDXXXXXXXXSKMCNLPRHTL 3128
               + C Q  L   + + L   +++R  AN S  CLCY +D         ++C L   ++
Sbjct: 500  ITPEECPQFILQTSEGAQLLSRSNQRMPANDSVGCLCYVYDIDQSSEN--EICKLEETSV 557

Query: 3127 LPLAQRVMSTFFXXXXXXXXXXXXXLQKL--KACYIALDKNVDSALTNCAEREDYLSQMI 2954
              LA+ + +T+F             L K     C +  DK   S   +  E+E  LS  +
Sbjct: 558  HTLAKYIRTTYFTTKSGCSELKSISLLKKLRNLCAVLSDKENMSVSNDNCEQEKNLSYTL 617

Query: 2953 VEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGDMSFDYLTVL-KRLQTF 2777
             + +  L+  + +STFEFIESG +R L  YL+NG Y +R +D +   ++L VL KR + F
Sbjct: 618  GQILEELSGEEPVSTFEFIESGIVRSLVDYLSNGCYRNRVVDPNELLNHLLVLQKRYEVF 677

Query: 2776 SFKLLS--GHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHP 2603
            +   LS   H  +  PL  L+  LQ+ALS LE FPVVL   +K K     IP + +T HP
Sbjct: 678  AKLCLSPPSHPLKDKPLGILLGKLQSALSLLENFPVVLHDVSKPKETYVTIPFERTTFHP 737

Query: 2602 CLQVHFVTEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXTQRHIK 2426
            CL+V F+ EEG+T LC Y  +V++++  +S +AIE +LWP                +H  
Sbjct: 738  CLKVRFIKEEGDTSLCDYVSDVVSIEAFTSINAIERYLWPRV--------------KHEA 783

Query: 2425 QSSRK---VDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAILQDKINEDPD 2255
            +S+ +   V +++   S+ TQ        KL+F LD  QLD+++TLYQAILQ K+  + D
Sbjct: 784  ESATEECAVATEDLKSSNDTQ--------KLLFYLDKGQLDQTLTLYQAILQLKMETEND 835

Query: 2254 IIVSKKFWNEVHRVTLWS---AKKLQLIYPQISADPRQSTLSWDKTELPWHNNFSFFSSM 2084
            + V   FW +V+ +        KK+ +  P +  +   S   W   ++ +  N   FSSM
Sbjct: 836  LSVGPSFWKQVYEIKYTKDTGQKKINI--PDVHHNSGSSR-EWSHPQI-YLQNAMCFSSM 891

Query: 2083 LLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAH 1904
            L +E P   ++S+  Y++L +LKILE LNR +  L  +ER ++FAEG  ++ D+L V   
Sbjct: 892  LFSEPPLSFKKSSTIYEILTLLKILEVLNRSASHLVYHERISSFAEGCDDSLDNLIVPVC 951

Query: 1903 SIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIF 1724
             +PQTEF+N KLT KLEQQM D   +ST ++P WC+QL+ A PFLF FE R KYF + + 
Sbjct: 952  GVPQTEFVNLKLTGKLEQQMQDASVVSTGSIPSWCVQLMEAFPFLFGFETRCKYFQMTLV 1011

Query: 1723 GVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEI 1544
              S  Q  N        I+DR+S ++  PRKKFKV R+ IL SAA+ M  + G+K +LE+
Sbjct: 1012 NSSEVQPDN--------IDDRQSQASGSPRKKFKVCRSRILESAAQAMSSHVGRKAILEV 1063

Query: 1543 EFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFIIVHSG 1364
            E+  EVGTG GPT+EF+TLVS  FQKVGMGMWRGD   T+    ++  V  SGF++   G
Sbjct: 1064 EYPEEVGTGQGPTMEFFTLVSQEFQKVGMGMWRGDPKLTSM---SNSEVDSSGFVVAPFG 1120

Query: 1363 LFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEKDLD 1184
            LFPRPWS      N    S+V+K F LLGQL A+A+QDGR+LDLP S + YKL+LE+DL+
Sbjct: 1121 LFPRPWS-ASSAPNETQLSEVVKKFVLLGQLVAKALQDGRVLDLPLSCSLYKLILEQDLN 1179

Query: 1183 IYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEELCLDFSLPG 1004
            +YDI SFD  +GR ++EFQAL  RK  L+S S    + R D  F NTRIE+LCLDF+LPG
Sbjct: 1180 LYDIFSFDHGVGRALVEFQALVDRKKVLDSVS----VKRSDSYFQNTRIEDLCLDFTLPG 1235

Query: 1003 YPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFT 824
            YPDY  +S+   +MVN+SNL+EYVS  +DATV +GI RQ+E+FK GF++V P+K+L+IFT
Sbjct: 1236 YPDYKLTSVDGHEMVNMSNLQEYVSSTLDATVNNGICRQVEAFKLGFNQVLPLKSLKIFT 1295

Query: 823  AEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQRRAFLLFVT 644
            AEEL++LLCGEQ+ W+  EL +HI FDHGY+++S   +K LEI+QEF+  QRRAFL FVT
Sbjct: 1296 AEELDQLLCGEQNAWNYNELLDHIKFDHGYTASSPTIIKLLEIMQEFEYNQRRAFLQFVT 1355

Query: 643  GAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYA 464
            GAPRLPPGGLAAL PKLTIVCK C   AD +LPSVMTCANYLKLPPYSSKE M+E+LLYA
Sbjct: 1356 GAPRLPPGGLAALNPKLTIVCKPCKGGADGDLPSVMTCANYLKLPPYSSKEIMKERLLYA 1415

Query: 463  IREGQGAFHLS 431
            I EGQG+F LS
Sbjct: 1416 ITEGQGSFLLS 1426


>ref|XP_023527254.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita pepo
            subsp. pepo]
          Length = 1489

 Score = 1084 bits (2803), Expect = 0.0
 Identities = 622/1403 (44%), Positives = 878/1403 (62%), Gaps = 19/1403 (1%)
 Frame = -1

Query: 4582 LRSVG--GDPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVML 4409
            L S+G   D SAQI  LT  C+ L+F   +  L+    D +  +LV L+     S +++L
Sbjct: 110  LSSLGEESDTSAQILLLTDLCEVLSFCMESS-LSSTTSDSLSIILVNLVKLDSNS-EIVL 167

Query: 4408 MSARVLTYFLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVP 4229
            ++ R LTY  D  PRA+   +++HG V ALC  L  +VYLDVAEQCL+AL+++S+   V 
Sbjct: 168  LALRALTYLCDAYPRASS-FIVRHGGVPALCQRLGVIVYLDVAEQCLQALERISQEHPVA 226

Query: 4228 CLRAGVINLVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDR 4049
            CL  G +  VL+F+DFF  S QR AL TV+N+CK++P +C   +++AVP+LCNL  + D 
Sbjct: 227  CLEGGAVMAVLTFIDFFSSSIQRTALRTVVNICKKLPSECPQTLIEAVPILCNLFQHDDG 286

Query: 4048 KLVESTAMCLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELL 3869
            +LVE+ A C+++I E    S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +L
Sbjct: 287  ELVENVARCMIKIAECAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGIL 346

Query: 3868 VRLANRSLLSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLI 3689
            V+L++ S+++ ++LYE +I++ L+ IL   + S         D  R+QV   LKLLN+L+
Sbjct: 347  VKLSSGSIVAFKTLYELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELL 406

Query: 3688 PPVARDDQDIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKN 3509
            P    +D   + +  K   +   PK   +F + ILP L Q V+SGA   +   C+++I  
Sbjct: 407  PT---EDAKAEQLSEKVSFLASNPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYK 463

Query: 3508 YVLIATPDMLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKE 3329
            ++ +   DML  L++NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKE
Sbjct: 464  FICLGESDMLVELLQNANISSFLVGVFTRKDHHVLMLALKITEIILQKLCSIFLKSFVKE 523

Query: 3328 GVSHAIRVLSKQDGCYQS--QLMDQQSSNLGDTDERTAANLSRCLCYAFDXXXXXXXXSK 3155
            GV  AI  L   +   Q    +      + G   + T  N  RCLCYAF           
Sbjct: 524  GVYFAIGALITPEKYKQLIFPVFAGVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAET 582

Query: 3154 -MCNLPRHTLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTNC-- 2987
              C L   ++  LA  + + +F             + Q L     ALD  ++ +L     
Sbjct: 583  GSCKLDDDSVYSLANHIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTP 642

Query: 2986 AEREDYLSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGD-MSFD 2810
            A+ E+    ++ E ++ L  G+ ISTFEFIESG ++   +YLTNG YL +  +   +S  
Sbjct: 643  AQDEEKFYALLAEIMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQ 702

Query: 2809 YLTVLKRLQTFSFKLLSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRST 2636
            +  + +R + F+  L S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++   
Sbjct: 703  FSIMERRFEAFARLLFSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFA 762

Query: 2635 DIPAKSSTKHPCLQVHFVTEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXXX 2459
             +P      HPC++V FV  +GETDLC +  ++LTVD  SS +AIE FLWP         
Sbjct: 763  TVPNGRCIPHPCVKVRFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVSRKKAEQ 822

Query: 2458 XXXXXTQRH-----IKQSSRKVDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLY 2294
                 + R      +   S +V + E+   S + + +G  +PKL+  L+GKQL+ ++TLY
Sbjct: 823  SSEADSLREHQVKLLSNVSVEVSTDEKSQGSASSSKKGT-KPKLLLYLEGKQLEPTLTLY 881

Query: 2293 QAILQDKINEDPDIIVSKKFWNEVHRVTLWSAKKLQ--LIYPQISADPRQSTLSWDKTEL 2120
            Q ILQ  I E+ + I   K W++V+ +    A +++        SA  + +TL +     
Sbjct: 882  QTILQQHIKEN-EAISGTKVWSQVYTIMYKRAGEVEDNSCNQFFSASDKGATLHFS---- 936

Query: 2119 PWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGT 1940
                  SFF  +L  +LP  L R +P YD+LF+L+I+EG+NR++F + S+ER  AF+EG 
Sbjct: 937  ------SFFCGILDCDLPSDLARESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEGR 990

Query: 1939 LENFDDLKVIAHSIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSF 1760
            +   D++K+   S+ Q EF+NSKLT KLEQQM D  A+S   MPLWC +L+ +CPFLFSF
Sbjct: 991  ISTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFSF 1050

Query: 1759 EARWKYFCLNIFGVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMM 1580
            EAR KYF + +FG+ +     S  +++ T N  RS S  LPRKK  V R+ IL SAAKMM
Sbjct: 1051 EARRKYFRIVVFGMPQQPYVRSY-SDLGTSNGVRSSSGGLPRKKALVLRDKILLSAAKMM 1109

Query: 1579 QWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMV 1400
              YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  FQK G+GMWRGDH +     G S+ 
Sbjct: 1110 DQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP--GKSLN 1167

Query: 1399 VSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSR 1220
            +     I    GLFPRPW + +D    + FS+V+K F L+GQ+ A+AIQDGR++D+ FS+
Sbjct: 1168 IEGRETIESPFGLFPRPWLSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRVMDIYFSK 1226

Query: 1219 AFYKLMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTR 1040
            AFYKL+L +++ IYDIQSFDPELG  ++EFQAL  R   LES   E+S  R +  +H+T 
Sbjct: 1227 AFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSRLEFCYHDTN 1286

Query: 1039 IEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFS 860
            IE+LCLDF+LPGYPD   +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E+FKSGF+
Sbjct: 1287 IEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFN 1346

Query: 859  KVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFD 680
            +VFPI+ LQ+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S   +  LEIIQ+FD
Sbjct: 1347 QVFPIEHLQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFD 1406

Query: 679  ARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYS 500
              Q+RAFL FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMTCANYLKLPPYS
Sbjct: 1407 NEQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYS 1466

Query: 499  SKERMREKLLYAIREGQGAFHLS 431
            SKE M+EKLLYAI EGQG+FHLS
Sbjct: 1467 SKEIMKEKLLYAITEGQGSFHLS 1489


>ref|XP_022983502.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita maxima]
          Length = 1489

 Score = 1082 bits (2799), Expect = 0.0
 Identities = 620/1403 (44%), Positives = 879/1403 (62%), Gaps = 19/1403 (1%)
 Frame = -1

Query: 4582 LRSVG--GDPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVML 4409
            L S+G   D SAQI  LT  C+ L+F   +  L+    D +  +LV L+     S +++L
Sbjct: 110  LSSLGEESDTSAQILLLTDLCEVLSFCMESS-LSSTTSDSLSIILVNLVKLDSNS-EIVL 167

Query: 4408 MSARVLTYFLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVP 4229
            ++ R LTY  D  PRA+   +++HG V ALC  L  +VYLDVAEQCL+AL+++S+   V 
Sbjct: 168  LALRALTYLCDAYPRASS-FIVRHGGVPALCQRLGVIVYLDVAEQCLQALERISQEHPVA 226

Query: 4228 CLRAGVINLVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDR 4049
            CL  G +  VL+F+DFF  S QR AL TV+N+CK++P +C   +++AVP+LCNL  + D 
Sbjct: 227  CLEGGAVMAVLTFIDFFSSSIQRTALRTVVNICKKLPSECPLTLIEAVPILCNLFQHDDG 286

Query: 4048 KLVESTAMCLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELL 3869
            +LVE+ A C+++I E    S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +L
Sbjct: 287  ELVENVARCMIKIAECAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGIL 346

Query: 3868 VRLANRSLLSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLI 3689
            V+L++ S+++ ++LYE +I++ L+ IL   + S         D  R+QV   LKLLN+L+
Sbjct: 347  VKLSSGSIVAFKTLYELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELL 406

Query: 3688 PPVARDDQDIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKN 3509
            P    +D   + +  K   +   PK   +F + ILP L Q V+SGA   +   C+++I  
Sbjct: 407  PT---EDAKAEQLSEKVSFLAINPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYK 463

Query: 3508 YVLIATPDMLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKE 3329
            ++ +   DML  L++NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKE
Sbjct: 464  FICLGESDMLVELLQNANISSFLVGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKE 523

Query: 3328 GVSHAIRVLSKQDGCYQS--QLMDQQSSNLGDTDERTAANLSRCLCYAFDXXXXXXXXSK 3155
            GV  AI  L   +   Q    +      + G   + T  N  RCLCYAF           
Sbjct: 524  GVYFAIDALITPEKYKQLIFPVFAGVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAET 582

Query: 3154 -MCNLPRHTLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTNC-- 2987
              C L + ++  LA  + + +F             + Q L     ALD  ++ +L     
Sbjct: 583  GSCKLDKDSVYSLANHIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTP 642

Query: 2986 AEREDYLSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGD-MSFD 2810
            A+ E+    ++ E ++ L  G+ ISTFEFIESG ++   +YLTNG YL +  +   +S  
Sbjct: 643  AQDEEKFYTLLAEIMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQ 702

Query: 2809 YLTVLKRLQTFSFKLLSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRST 2636
            +  + +R + F+  L S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++   
Sbjct: 703  FSIMERRFEAFARLLFSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFA 762

Query: 2635 DIPAKSSTKHPCLQVHFVTEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXXX 2459
             +P      HPC++V FV  +GETDLC +  ++LTVD  SS +AIE FLWP         
Sbjct: 763  TVPNGRCIPHPCVKVRFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVTRKKAEQ 822

Query: 2458 XXXXXTQRH-----IKQSSRKVDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLY 2294
                 + R      +   S +V + E+   S + + +G  +PKL+  L+GKQL+ ++TLY
Sbjct: 823  SSEADSLREHQVKPLSNVSVEVSTDEKSQGSASSSKKGT-KPKLLLYLEGKQLEPTLTLY 881

Query: 2293 QAILQDKINEDPDIIVSKKFWNEVHRVTLWSAKKLQ--LIYPQISADPRQSTLSWDKTEL 2120
            Q ILQ  I E+ + I   K W++V+ +   SA +++        SA  + +TL +     
Sbjct: 882  QTILQQHIKEN-EAISGTKVWSQVYTIMYKSAGEVEDNSCNQFFSASDKGATLHFS---- 936

Query: 2119 PWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGT 1940
                  SFF  +L  +LP  L + +P YD+LF+L+I+EG+NR++F + S+ER  AF+EG 
Sbjct: 937  ------SFFCGILDCDLPSDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEGR 990

Query: 1939 LENFDDLKVIAHSIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSF 1760
            +   +++K+   S+ Q EF+NSKLT KLEQQM D  A+S   MPLWC +L+ +CPFLFSF
Sbjct: 991  ISTLNNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFSF 1050

Query: 1759 EARWKYFCLNIFGVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMM 1580
            EAR KYF + +FG+ +     S  +++ T N  RS S   PRKK  V RN IL SAAKMM
Sbjct: 1051 EARRKYFRIVVFGMPQQPYVRSY-SDLGTSNGVRSSSGGFPRKKALVLRNQILLSAAKMM 1109

Query: 1579 QWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMV 1400
              YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  FQK G+GMWRGDH +     G S+ 
Sbjct: 1110 DQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP--GKSLN 1167

Query: 1399 VSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSR 1220
            +     I    GLFPRPW + +D    + FS+V+K F L+GQ+ A+AIQDGR++D+ FS+
Sbjct: 1168 IEGRETIESPFGLFPRPWLSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRVMDIYFSK 1226

Query: 1219 AFYKLMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTR 1040
            AFYKL+L +++ IYDIQSFDPELG  ++EFQAL  R   LES   E+S  + +  +H+T 
Sbjct: 1227 AFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSKLEFCYHDTN 1286

Query: 1039 IEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFS 860
            IE+LCLDF+LPGYPD   +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E+FKSGF+
Sbjct: 1287 IEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFN 1346

Query: 859  KVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFD 680
            +VFPI+ LQ+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S   +  LEIIQ+FD
Sbjct: 1347 QVFPIEHLQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFD 1406

Query: 679  ARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYS 500
              Q+RAFL FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMTCANYLKLPPYS
Sbjct: 1407 NEQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYS 1466

Query: 499  SKERMREKLLYAIREGQGAFHLS 431
            SKE M+EKLLYAI EGQG+FHLS
Sbjct: 1467 SKEIMKEKLLYAITEGQGSFHLS 1489


>ref|XP_004137960.2| PREDICTED: E3 ubiquitin-protein ligase UPL4 [Cucumis sativus]
 gb|KGN58932.1| hypothetical protein Csa_3G736800 [Cucumis sativus]
          Length = 1506

 Score = 1077 bits (2786), Expect = 0.0
 Identities = 620/1418 (43%), Positives = 883/1418 (62%), Gaps = 34/1418 (2%)
 Frame = -1

Query: 4582 LRSVG--GDPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVML 4409
            L S+G   + S Q   L   C+ L+F    + ++ +  D +  +LV L+     S D++L
Sbjct: 110  LTSLGEESESSVQTELLRELCEVLSFCT-ENSISSMTSDSLSIILVNLVKLDSDS-DIVL 167

Query: 4408 MSARVLTYFLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVP 4229
            ++ R LTY  D  PRA+   +++HG V A C  L  + Y DVAEQC +AL+K+S+   V 
Sbjct: 168  LALRALTYLCDAYPRASS-FIVRHGGVPAFCKRLGAIEYSDVAEQCFQALEKISQEHPVA 226

Query: 4228 CLRAGVINLVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDR 4049
            CL  G +  VL+F+DFF    QR AL  V+NVCK++P +C   +++AVP+LCNLL Y D 
Sbjct: 227  CLEGGAVMAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPQNLIEAVPILCNLLQYDDE 286

Query: 4048 KLVESTAMCLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELL 3869
            +LVE+ A C+++I E    SSELLD LC+HG+++ +++LI+ +S   LS+T Y  ++ +L
Sbjct: 287  ELVENVARCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVL 346

Query: 3868 VRLANRSLLSIRSLYESNINSILESILRNPDHSRRTPPSHCE-DISRDQVYVALKLLNQL 3692
            ++LA+ S+++  +LYE NI++ L+ IL   + S     S    D  R+QV   LKLLN+L
Sbjct: 347  IKLASGSIVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNEL 406

Query: 3691 IPPVARDDQDIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIK 3512
            +P    +D   + +  K   ++  PK   +F + ILP L Q V+SGAN  +   C+++I 
Sbjct: 407  LPT---EDAKTEQLSEKVSFLVSNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIY 463

Query: 3511 NYVLIATPDMLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVK 3332
             +V +   DML  L++N+N+  FLAG+ +RKDHHVL+  L + EII++KL    L SFVK
Sbjct: 464  KFVCLGESDMLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASTFLKSFVK 523

Query: 3331 EGVSHAIRVLSKQDGCYQS--QLMDQQSSNLGDTDERTAANLSRCLCYAFDXXXXXXXXS 3158
            EGV  +I  L   D   Q    +     S+ G   ++++    RCLCYAF          
Sbjct: 524  EGVYFSIDALISPDKYKQLIFPVFTGVHSSFGSC-QKSSREHGRCLCYAFSSSCFPSVSE 582

Query: 3157 K-MCNLPRHTLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTNC- 2987
               C L + ++  LA  + S +F             + Q L+    ALD  ++ +L    
Sbjct: 583  TGSCKLDKDSVYSLANHIRSIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDT 642

Query: 2986 -AEREDYLSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGD-MSF 2813
             A+ E+ L  ++ E ++ L  G+ ISTFEFIESG ++   +Y+TNG YL +  +   +S 
Sbjct: 643  PAQDEEKLYALLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKGESQPISR 702

Query: 2812 DYLTVLKRLQTFSFKLLSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAAN-KSKSR 2642
             +  + +R + F+  LLS   H   + P+ +L++ LQ +LS+LE F V++S+   K ++ 
Sbjct: 703  HFSIIERRFEAFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFHVIISSQGFKHRNY 762

Query: 2641 STDIPAKSSTKHPCLQVHFVTEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXX 2465
               +P      HPC++V FV  +GETDLC +  ++L VD  SS  AIE FLWP       
Sbjct: 763  FVTVPNARCVPHPCVKVRFVRGDGETDLCDINGDILNVDPFSSLTAIEGFLWPKVSSQKT 822

Query: 2464 XXXXXXXTQRH-IKQSSR------------------KVDSKERVGSSTTQNNEGDIEPKL 2342
                    + H IK  S+                  +V + E+   S + + +G   P+L
Sbjct: 823  EQSPEDTLREHQIKLLSKLVGSDIMSTDLPEVQVPAEVSADEKSQCSASCSKKGTA-PRL 881

Query: 2341 IFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLWSAKKLQLIYPQISA 2162
            +  L+GKQL+ ++++YQAILQ  I E+ + I   K W++V+ +   SA +++       +
Sbjct: 882  LLYLEGKQLEPTLSIYQAILQQHIKEN-ETISGIKIWSQVYTIMYRSAGEVE------DS 934

Query: 2161 DPRQSTLSWDKT-ELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSF 1985
               Q   + DK  +L +    SFF  +L   LP  L + +PAYD+LF+L+ +EG+NR++F
Sbjct: 935  TCNQLFCASDKALKLQFS---SFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAF 991

Query: 1984 QLFSNERSNAFAEGTLENFDDLKVIAHSIPQTEFINSKLTSKLEQQMHDNLALSTATMPL 1805
             + S+ER  AFA+G ++  D++K+   S+ Q EF+NSKLT KLEQQM D  A+S   MPL
Sbjct: 992  HIMSHERIRAFADGKIDTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSVGGMPL 1051

Query: 1804 WCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTQNSEKNEISTINDRRSLSASLPRKKF 1625
            WC +L+ +CPFLFSFEAR KYF + +FG+ + Q      ++  T ND RS S  LPRKK 
Sbjct: 1052 WCKELMDSCPFLFSFEARRKYFRIVVFGMPQYQLHARSHSDFGTSNDGRSSSGGLPRKKV 1111

Query: 1624 KVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWR 1445
             VHR+ IL SA+KMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  FQK G+GMWR
Sbjct: 1112 LVHRSQILDSASKMMNQYANQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKNGLGMWR 1171

Query: 1444 GDHTSTAKRLGTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTA 1265
            GDH +     G  + + D        GLFPRPW + +D ++ +   +V+K F LLGQ+ A
Sbjct: 1172 GDHDAFIS--GKRLNIEDRETTESPFGLFPRPWPSTLD-TDKLHLPEVMKKFVLLGQIVA 1228

Query: 1264 RAIQDGRILDLPFSRAFYKLMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSG 1085
            +AIQD R+LD+ FS+AFYKL+L ++L IYDIQSFDPELG  ++EFQAL  R   L S   
Sbjct: 1229 KAIQDCRVLDIYFSKAFYKLILGQELSIYDIQSFDPELGTVLLEFQALVNRNKLLGSVYE 1288

Query: 1084 EHSLDRFDPIFHNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVR 905
            E+S  + +  +HNT IE+LCLDF+LPGYPDY+ +S  D+ MVN  NLE YVSLV DAT+ 
Sbjct: 1289 ENSSSKLEFSYHNTNIEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLENYVSLVADATLC 1348

Query: 904  SGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSST 725
            SGISRQ+E+FKSGF++VFPI+ LQ+FTAEELERL+CGEQD W + +L +++ FDHGY+S+
Sbjct: 1349 SGISRQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSS 1408

Query: 724  SHPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELP 545
            S   +  LEIIQ+FD +Q+RAFL FVTGAPRLP GG A+L PKLTIV KH  N  D +LP
Sbjct: 1409 SPSIVHLLEIIQDFDNKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLP 1468

Query: 544  SVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 431
            SVMTCANYLKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1469 SVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1506


>ref|XP_022934795.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita moschata]
          Length = 1489

 Score = 1077 bits (2784), Expect = 0.0
 Identities = 617/1404 (43%), Positives = 879/1404 (62%), Gaps = 20/1404 (1%)
 Frame = -1

Query: 4582 LRSVG--GDPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVML 4409
            L S+G   D SAQI  LT  C+ L+F   +  L+    D +  +LV L+     S +++L
Sbjct: 110  LSSLGEESDTSAQILLLTDLCEVLSFCMESS-LSSTTSDSLSIILVNLVKLDSNS-EIVL 167

Query: 4408 MSARVLTYFLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVP 4229
            ++ R LTY  D  PRA+   +++HG V ALC  L  +VYLDVAEQCL+AL+++S+   V 
Sbjct: 168  LALRALTYLCDAYPRASS-FIVRHGGVPALCQRLGVIVYLDVAEQCLQALERISQEHPVA 226

Query: 4228 CLRAGVINLVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDR 4049
            CL +G +  VL+F+DFF  S QR AL TV+N+CK++P +C   +++AVP+LCNL  + D 
Sbjct: 227  CLESGAVMAVLTFIDFFSSSIQRTALRTVVNICKKLPSECPQTLIEAVPILCNLFQHDDG 286

Query: 4048 KLVESTAMCLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELL 3869
            +LVE+ A C+++I E    S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +L
Sbjct: 287  ELVENVARCMIKIAECAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGIL 346

Query: 3868 VRLANRSLLSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLI 3689
            V+L++ S+++ ++LYE +I++ L+ IL   + S         D  R+QV   LKLLN+L+
Sbjct: 347  VKLSSGSIVAFKTLYELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELL 406

Query: 3688 PPVARDDQDIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKN 3509
            P    +D   + +  K   +   PK   +F + ILP L Q V+SGA   +   C+++I  
Sbjct: 407  PT---EDAKAEQLSEKVSFLASNPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYK 463

Query: 3508 YVLIATPDMLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKE 3329
            ++ +   DML  L++NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKE
Sbjct: 464  FICLGESDMLVELLQNANISSFLVGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKE 523

Query: 3328 GVSHAIRVLSKQDGCYQSQLMDQQSS---NLGDTDERTAANLSRCLCYAFDXXXXXXXXS 3158
            GV  AI  L   +  Y+  +    +S   + G   + T  N  RCLCYAF          
Sbjct: 524  GVYFAIDALITPEK-YKQLIFPVFASVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAE 581

Query: 3157 K-MCNLPRHTLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTNCA 2984
               C L   ++  LA  + + +F             + Q L     ALD  ++ +L    
Sbjct: 582  TGSCKLDDDSVYSLANHIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDT 641

Query: 2983 --EREDYLSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGD-MSF 2813
              + E+    ++ E ++ L  G+ ISTFEFIESG ++   +YLTNG YL +  +   +S 
Sbjct: 642  PGQDEEKFYALLAEIMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISR 701

Query: 2812 DYLTVLKRLQTFSFKLLSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRS 2639
             +  + +R + F+  L S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++  
Sbjct: 702  QFSIMERRFEAFARLLFSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYF 761

Query: 2638 TDIPAKSSTKHPCLQVHFVTEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXX 2462
              +P      HPC++V FV  +GETDLC +  ++LTVD  SS +AIE FLWP        
Sbjct: 762  ATVPNGRCIPHPCVKVRFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVSRKKAE 821

Query: 2461 XXXXXXTQRH-----IKQSSRKVDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTL 2297
                  + R      +  +S +V + E+   S + + +G  +PKL+  L+GKQL+ ++TL
Sbjct: 822  QSSEADSLREHQVKLLSNASVEVSTDEKSLGSASSSKKGT-KPKLLLYLEGKQLEPTLTL 880

Query: 2296 YQAILQDKINEDPDIIVSKKFWNEVHRVTLWSAKKLQ--LIYPQISADPRQSTLSWDKTE 2123
            YQ ILQ  I E+ + I   K W++V+ +    A +++        S   + +TL +    
Sbjct: 881  YQTILQRHIKEN-EAISGTKVWSQVYTIMYKRAGEVEDNSCNQFFSGSDKGATLHFS--- 936

Query: 2122 LPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEG 1943
                   SFF  +L  +LP  L + +P YD+LF+L+I+EG+NR++F + S+ER  AF+EG
Sbjct: 937  -------SFFCGILDCDLPSDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEG 989

Query: 1942 TLENFDDLKVIAHSIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFS 1763
             +   D++K+   S+ Q EF+NSKLT KLEQQM D  A+S   MPLWC +L+ +CPFLFS
Sbjct: 990  RISTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFS 1049

Query: 1762 FEARWKYFCLNIFGVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKM 1583
            FEAR KYF + +FG+ +     S  +++ T N  RS S  LPRKK  V R+ IL SAAKM
Sbjct: 1050 FEARRKYFRIVVFGMPQQPYVRSY-SDLGTSNGVRSSSGGLPRKKALVLRDKILLSAAKM 1108

Query: 1582 MQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSM 1403
            M  YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  FQK G+GMWRGDH +     G S+
Sbjct: 1109 MDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP--GKSL 1166

Query: 1402 VVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFS 1223
             +     I    GLFPRPW + +D    + FS+V+K F L+GQ+ A+AIQDGR++D+ FS
Sbjct: 1167 NIEGRETIESPFGLFPRPWLSTVDIGE-LQFSEVIKRFTLMGQIVAKAIQDGRVMDIYFS 1225

Query: 1222 RAFYKLMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNT 1043
            +AFYKL+L +++ IYDIQSFDPELG  ++EFQAL  R   LES   E+S  + +  +H+T
Sbjct: 1226 KAFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSKLEFCYHDT 1285

Query: 1042 RIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGF 863
             IE+LCLDF+LPGYPD   +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E+FKSGF
Sbjct: 1286 NIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGF 1345

Query: 862  SKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEF 683
            ++VFPI+ LQ+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S   +  LEIIQ+F
Sbjct: 1346 NQVFPIEHLQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDF 1405

Query: 682  DARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPY 503
            D  Q+RAFL FVTGAPRLP GG  +L PKLTIV KH  N  D +LPSVMTCANYLKLPPY
Sbjct: 1406 DNEQQRAFLQFVTGAPRLPSGGFTSLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPY 1465

Query: 502  SSKERMREKLLYAIREGQGAFHLS 431
            SSKE  +EKLLYAI EGQG+FHLS
Sbjct: 1466 SSKEITKEKLLYAITEGQGSFHLS 1489


>ref|XP_022934794.1| E3 ubiquitin-protein ligase UPL4-like isoform X2 [Cucurbita moschata]
          Length = 1504

 Score = 1066 bits (2758), Expect = 0.0
 Identities = 621/1428 (43%), Positives = 878/1428 (61%), Gaps = 44/1428 (3%)
 Frame = -1

Query: 4582 LRSVG--GDPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVML 4409
            L S+G   D SAQI  LT  C+ L+F   +  L+    D +  +LV L+     S +++L
Sbjct: 100  LSSLGEESDTSAQILLLTDLCEVLSFCMESS-LSSTTSDSLSIILVNLVKLDSNS-EIVL 157

Query: 4408 MSARVLTYFLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVP 4229
            ++ R LTY  D  PRA+   +++HG V ALC  L  +VYLDVAEQCL+AL+++S+   V 
Sbjct: 158  LALRALTYLCDAYPRASS-FIVRHGGVPALCQRLGVIVYLDVAEQCLQALERISQEHPVA 216

Query: 4228 CLRAGVINLVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDR 4049
            CL +G +  VL+F+DFF  S QR AL TV+N+CK++P +C   +++AVP+LCNL  + D 
Sbjct: 217  CLESGAVMAVLTFIDFFSSSIQRTALRTVVNICKKLPSECPQTLIEAVPILCNLFQHDDG 276

Query: 4048 KLVESTAMCLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELL 3869
            +LVE+ A C+++I E    S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +L
Sbjct: 277  ELVENVARCMIKIAECAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGIL 336

Query: 3868 VRLANRSLLSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLI 3689
            V+L++ S+++ ++LYE +I++ L+ IL   + S         D  R+QV   LKLLN+L+
Sbjct: 337  VKLSSGSIVAFKTLYELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELL 396

Query: 3688 PPVARDDQDIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKN 3509
            P    +D   + +  K   +   PK   +F + ILP L Q V+SGA   +   C+++I  
Sbjct: 397  P---TEDAKAEQLSEKVSFLASNPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYK 453

Query: 3508 YVLIATPDMLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKE 3329
            ++ +   DML  L++NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKE
Sbjct: 454  FICLGESDMLVELLQNANISSFLVGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKE 513

Query: 3328 GVSHAIRVLSKQDGCYQSQLMDQQSS---NLGDTDERTAANLSRCLCYAFDXXXXXXXXS 3158
            GV  AI  L   +  Y+  +    +S   + G   + T  N  RCLCYAF          
Sbjct: 514  GVYFAIDALITPEK-YKQLIFPVFASVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAE 571

Query: 3157 K-MCNLPRHTLLPLAQRVMSTFF-XXXXXXXXXXXXXLQKLKACYIALDK--NVDSALTN 2990
               C L   ++  LA  + + +F              LQ L     ALD   N+      
Sbjct: 572  TGSCKLDDDSVYSLANHIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDT 631

Query: 2989 CAEREDYLSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGD-MSF 2813
              + E+    ++ E ++ L  G+ ISTFEFIESG ++   +YLTNG YL +  +   +S 
Sbjct: 632  PGQDEEKFYALLAEIMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISR 691

Query: 2812 DYLTVLKRLQTFSFKLLSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRS 2639
             +  + +R + F+  L S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++  
Sbjct: 692  QFSIMERRFEAFARLLFSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYF 751

Query: 2638 TDIPAKSSTKHPCLQVHFVTEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXX 2462
              +P      HPC++V FV  +GETDLC +  ++LTVD  SS +AIE FLWP        
Sbjct: 752  ATVPNGRCIPHPCVKVRFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVSRKKAE 811

Query: 2461 XXXXXXTQR--HIKQSSRKVD----SKERVGSSTTQNNEGDIE----------------- 2351
                  + R   +K  S        + E VGS++  ++  +IE                 
Sbjct: 812  QSSEADSLREHQVKLLSNVCSYSGVNPELVGSNSKSSDLPEIEASVEVSTDEKSLGSASS 871

Query: 2350 ------PKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLWSAKKL 2189
                  PKL+  L+GKQL+ ++TLYQ ILQ  I E+ + I   K W++V+ +    A ++
Sbjct: 872  SKKGTKPKLLLYLEGKQLEPTLTLYQTILQRHIKEN-EAISGTKVWSQVYTIMYKRAGEV 930

Query: 2188 Q--LIYPQISADPRQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLK 2015
            +        S   + +TL +           SFF  +L  +LP  L + +P YD+LF+L+
Sbjct: 931  EDNSCNQFFSGSDKGATLHFS----------SFFCGILDCDLPSDLAKESPVYDVLFLLR 980

Query: 2014 ILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAHSIPQTEFINSKLTSKLEQQMHDN 1835
            I+EG+NR++F + S+ER  AF+EG +   D++K+   S+ Q EF+NSKLT KLEQQM D 
Sbjct: 981  IIEGMNRMAFHIMSHERIRAFSEGRISTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDF 1040

Query: 1834 LALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTQNSEKNEISTINDRRS 1655
             A+S   MPLWC +L+ +CPFLFSFEAR KYF + +FG+ + Q      +++ T N  RS
Sbjct: 1041 SAVSIGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNGVRS 1099

Query: 1654 LSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHA 1475
             S  LPRKK  V R+ IL SAAKMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  
Sbjct: 1100 SSGGLPRKKALVLRDKILLSAAKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQE 1159

Query: 1474 FQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLK 1295
            FQK G+GMWRGDH +     G S+ +     I    GLFPRPW + +D    + FS+V+K
Sbjct: 1160 FQKYGLGMWRGDHDAFIP--GKSLNIEGRETIESPFGLFPRPWLSTVDIGE-LQFSEVIK 1216

Query: 1294 NFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEKDLDIYDIQSFDPELGRTMIEFQALAK 1115
             F L+GQ+ A+AIQDGR++D+ FS+AFYKL+L +++ IYDIQSFDPELG  ++EFQAL  
Sbjct: 1217 RFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVN 1276

Query: 1114 RKGFLESKSGEHSLDRFDPIFHNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEY 935
            R   LES   E+S  + +  +H+T IE+LCLDF+LPGYPD   +S  D+ MVN  NLE+Y
Sbjct: 1277 RSKLLESVCEENSSSKLEFCYHDTNIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDY 1336

Query: 934  VSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNH 755
            VSLV DAT+ SGIS Q+E+FKSGF++VFPI+ LQ+FTAEELERL+CGEQD+W + +L ++
Sbjct: 1337 VSLVADATLYSGISTQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEQDSWALSDLLDN 1396

Query: 754  INFDHGYSSTSHPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKH 575
            I FDHGY+++S   +  LEIIQ+FD  Q+RAFL FVTGAPRLP GG  +L PKLTIV KH
Sbjct: 1397 IKFDHGYTASSPSIINLLEIIQDFDNEQQRAFLQFVTGAPRLPSGGFTSLNPKLTIVRKH 1456

Query: 574  CDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 431
              N  D +LPSVMTCANYLKLPPYSSKE  +EKLLYAI EGQG+FHLS
Sbjct: 1457 SSNMVDSDLPSVMTCANYLKLPPYSSKEITKEKLLYAITEGQGSFHLS 1504


>ref|XP_022934791.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita moschata]
 ref|XP_022934792.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita moschata]
          Length = 1514

 Score = 1066 bits (2758), Expect = 0.0
 Identities = 621/1428 (43%), Positives = 878/1428 (61%), Gaps = 44/1428 (3%)
 Frame = -1

Query: 4582 LRSVG--GDPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVML 4409
            L S+G   D SAQI  LT  C+ L+F   +  L+    D +  +LV L+     S +++L
Sbjct: 110  LSSLGEESDTSAQILLLTDLCEVLSFCMESS-LSSTTSDSLSIILVNLVKLDSNS-EIVL 167

Query: 4408 MSARVLTYFLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVP 4229
            ++ R LTY  D  PRA+   +++HG V ALC  L  +VYLDVAEQCL+AL+++S+   V 
Sbjct: 168  LALRALTYLCDAYPRASS-FIVRHGGVPALCQRLGVIVYLDVAEQCLQALERISQEHPVA 226

Query: 4228 CLRAGVINLVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDR 4049
            CL +G +  VL+F+DFF  S QR AL TV+N+CK++P +C   +++AVP+LCNL  + D 
Sbjct: 227  CLESGAVMAVLTFIDFFSSSIQRTALRTVVNICKKLPSECPQTLIEAVPILCNLFQHDDG 286

Query: 4048 KLVESTAMCLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELL 3869
            +LVE+ A C+++I E    S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +L
Sbjct: 287  ELVENVARCMIKIAECAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGIL 346

Query: 3868 VRLANRSLLSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLI 3689
            V+L++ S+++ ++LYE +I++ L+ IL   + S         D  R+QV   LKLLN+L+
Sbjct: 347  VKLSSGSIVAFKTLYELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELL 406

Query: 3688 PPVARDDQDIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKN 3509
            P    +D   + +  K   +   PK   +F + ILP L Q V+SGA   +   C+++I  
Sbjct: 407  P---TEDAKAEQLSEKVSFLASNPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYK 463

Query: 3508 YVLIATPDMLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKE 3329
            ++ +   DML  L++NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKE
Sbjct: 464  FICLGESDMLVELLQNANISSFLVGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKE 523

Query: 3328 GVSHAIRVLSKQDGCYQSQLMDQQSS---NLGDTDERTAANLSRCLCYAFDXXXXXXXXS 3158
            GV  AI  L   +  Y+  +    +S   + G   + T  N  RCLCYAF          
Sbjct: 524  GVYFAIDALITPEK-YKQLIFPVFASVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAE 581

Query: 3157 K-MCNLPRHTLLPLAQRVMSTFF-XXXXXXXXXXXXXLQKLKACYIALDK--NVDSALTN 2990
               C L   ++  LA  + + +F              LQ L     ALD   N+      
Sbjct: 582  TGSCKLDDDSVYSLANHIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDT 641

Query: 2989 CAEREDYLSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGD-MSF 2813
              + E+    ++ E ++ L  G+ ISTFEFIESG ++   +YLTNG YL +  +   +S 
Sbjct: 642  PGQDEEKFYALLAEIMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISR 701

Query: 2812 DYLTVLKRLQTFSFKLLSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRS 2639
             +  + +R + F+  L S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++  
Sbjct: 702  QFSIMERRFEAFARLLFSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYF 761

Query: 2638 TDIPAKSSTKHPCLQVHFVTEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXX 2462
              +P      HPC++V FV  +GETDLC +  ++LTVD  SS +AIE FLWP        
Sbjct: 762  ATVPNGRCIPHPCVKVRFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVSRKKAE 821

Query: 2461 XXXXXXTQR--HIKQSSRKVD----SKERVGSSTTQNNEGDIE----------------- 2351
                  + R   +K  S        + E VGS++  ++  +IE                 
Sbjct: 822  QSSEADSLREHQVKLLSNVCSYSGVNPELVGSNSKSSDLPEIEASVEVSTDEKSLGSASS 881

Query: 2350 ------PKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLWSAKKL 2189
                  PKL+  L+GKQL+ ++TLYQ ILQ  I E+ + I   K W++V+ +    A ++
Sbjct: 882  SKKGTKPKLLLYLEGKQLEPTLTLYQTILQRHIKEN-EAISGTKVWSQVYTIMYKRAGEV 940

Query: 2188 Q--LIYPQISADPRQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLK 2015
            +        S   + +TL +           SFF  +L  +LP  L + +P YD+LF+L+
Sbjct: 941  EDNSCNQFFSGSDKGATLHFS----------SFFCGILDCDLPSDLAKESPVYDVLFLLR 990

Query: 2014 ILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAHSIPQTEFINSKLTSKLEQQMHDN 1835
            I+EG+NR++F + S+ER  AF+EG +   D++K+   S+ Q EF+NSKLT KLEQQM D 
Sbjct: 991  IIEGMNRMAFHIMSHERIRAFSEGRISTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDF 1050

Query: 1834 LALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTQNSEKNEISTINDRRS 1655
             A+S   MPLWC +L+ +CPFLFSFEAR KYF + +FG+ + Q      +++ T N  RS
Sbjct: 1051 SAVSIGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNGVRS 1109

Query: 1654 LSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHA 1475
             S  LPRKK  V R+ IL SAAKMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  
Sbjct: 1110 SSGGLPRKKALVLRDKILLSAAKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQE 1169

Query: 1474 FQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLK 1295
            FQK G+GMWRGDH +     G S+ +     I    GLFPRPW + +D    + FS+V+K
Sbjct: 1170 FQKYGLGMWRGDHDAFIP--GKSLNIEGRETIESPFGLFPRPWLSTVDIGE-LQFSEVIK 1226

Query: 1294 NFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEKDLDIYDIQSFDPELGRTMIEFQALAK 1115
             F L+GQ+ A+AIQDGR++D+ FS+AFYKL+L +++ IYDIQSFDPELG  ++EFQAL  
Sbjct: 1227 RFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVN 1286

Query: 1114 RKGFLESKSGEHSLDRFDPIFHNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEY 935
            R   LES   E+S  + +  +H+T IE+LCLDF+LPGYPD   +S  D+ MVN  NLE+Y
Sbjct: 1287 RSKLLESVCEENSSSKLEFCYHDTNIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDY 1346

Query: 934  VSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNH 755
            VSLV DAT+ SGIS Q+E+FKSGF++VFPI+ LQ+FTAEELERL+CGEQD+W + +L ++
Sbjct: 1347 VSLVADATLYSGISTQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEQDSWALSDLLDN 1406

Query: 754  INFDHGYSSTSHPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKH 575
            I FDHGY+++S   +  LEIIQ+FD  Q+RAFL FVTGAPRLP GG  +L PKLTIV KH
Sbjct: 1407 IKFDHGYTASSPSIINLLEIIQDFDNEQQRAFLQFVTGAPRLPSGGFTSLNPKLTIVRKH 1466

Query: 574  CDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 431
              N  D +LPSVMTCANYLKLPPYSSKE  +EKLLYAI EGQG+FHLS
Sbjct: 1467 SSNMVDSDLPSVMTCANYLKLPPYSSKEITKEKLLYAITEGQGSFHLS 1514


>ref|XP_023766631.1| LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase UPL4-like [Lactuca
            sativa]
          Length = 1392

 Score = 1030 bits (2664), Expect = 0.0
 Identities = 606/1392 (43%), Positives = 845/1392 (60%), Gaps = 14/1392 (1%)
 Frame = -1

Query: 4564 DPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVMLMSARVLTY 4385
            + SAQ   L   C+ L+F   +  L+ L  D + P+L++L A    +P++ML+S R LTY
Sbjct: 120  EESAQQEALRELCEILSFCTDSS-LSSLIADSLSPILIKL-ASHESNPEIMLLSIRALTY 177

Query: 4384 FLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVPCLRAGVIN 4205
              D+ P+++   +I+H  V ALC  L+   Y DVAEQCL+AL+K+SR Q + CL++G I 
Sbjct: 178  LCDIHPQSS-ALIIRHDGVTALCKRLLAFEYEDVAEQCLQALEKISREQPLACLQSGAIM 236

Query: 4204 LVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAM 4025
             VL+F+DFF  S QR+ALSTV+N+C ++P +  S  MDAVP+LC LL Y+D +LVE  A 
Sbjct: 237  AVLTFIDFFSTSLQRVALSTVVNICVKLPSEGSSPFMDAVPVLCKLLQYEDNQLVEHVAT 296

Query: 4024 CLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSL 3845
            CL++I E    SSE+LDEL KHG++ Q   LI  +S   LS + + G+I LLV+LA+ S+
Sbjct: 297  CLIKIAERVQESSEMLDELSKHGLIHQVAHLIDLNSHTTLSHSVHTGLIGLLVKLASGSM 356

Query: 3844 LSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQ 3665
            +++++L++ NI+SIL+ IL   D S   P     D   +Q +  LKLL QL+P V+R+ Q
Sbjct: 357  VAVKTLFDLNISSILKEILSIYDLSHGVPSPRTIDGHYNQXHEVLKLLIQLLPVVSRN-Q 415

Query: 3664 DIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKNYVLIATPD 3485
            ++ L   KE  ++  P L  +F   +LP L Q V SG +  I Y C+SV+   +  +  D
Sbjct: 416  EVPLAAEKEAFLVTHPDLVEKFGNDLLPVLIQVVDSGVDLYICYGCLSVVDKLLYYSNSD 475

Query: 3484 MLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRV 3305
            ML  L+K+ N+ +FLAG+ +RKD HVL+ ALN+ + I++K     +  FVKEGV  AI  
Sbjct: 476  MLLDLLKSTNISRFLAGVFTRKDIHVLMLALNISDTILQKNTDVFMGPFVKEGVLFAIDA 535

Query: 3304 LSKQDGCYQSQLMDQQSSNLGDTDERTAA--NLSRCLCYAFDXXXXXXXXS-KMCNLPRH 3134
            L   + C Q          L +T  +  A  ++ RCLC++FD          + C L   
Sbjct: 536  LIDPEKCSQFMFSMFNDIQLSNTSSKKYAGKDVIRCLCFSFDVNKTSSSSESRKCKLELE 595

Query: 3133 TLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTNCAEREDYLSQM 2957
             +  LA+ + + +F             + +KLK+        + S L++   +E Y   +
Sbjct: 596  CVRTLAKHIRTKYFDTNSSTSETGMTPILEKLKS--------LSSELSSMMTQEGY-DHV 646

Query: 2956 IVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGDMSFDYLTVLKRLQTF 2777
            + + ++ LN  D ISTFEF+ESG    L  YL+NGD                   R++ F
Sbjct: 647  LHQIMSILNGRDVISTFEFVESGITDSLVSYLSNGD-----------------CSRVEAF 689

Query: 2776 SFKLLS--GHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHP 2603
                LS       +F L+  +  LQ+ALS++E FPVVL+ + K ++    +P + ST +P
Sbjct: 690  GRLFLSCTNQPLDYFSLSGFISKLQSALSSVEDFPVVLNNSFKHRNSYATVPRRHSTTYP 749

Query: 2602 CLQVHFVTEEGETDLCLYD-NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXTQRHIK 2426
            CL+V FV EEGE+ L  Y  +V TVD  S ++AIE FLWP                    
Sbjct: 750  CLRVLFVKEEGESTLTEYTGDVQTVDPFSDFEAIETFLWPKVCLN--------------N 795

Query: 2425 QSSRKVDSKERVGSSTTQ----NNEGDIEPKLIFLLDGKQLDRSMTLYQAILQDKINEDP 2258
              S    S+  VG S T       +     KL F L+GK++DR +T+YQA+L   I+E  
Sbjct: 796  AQSPSDKSQHDVGQSQTSPQKPTEDASSSQKLSFYLEGKEIDRGLTIYQAVLSQHISEFD 855

Query: 2257 DIIVSKKFWNEVHRVTLWSAKKLQLIYPQISADPRQSTLSWDKTELPWHNNFSFFSSMLL 2078
               V+   WN++H++T                   + +L+ +K+  P             
Sbjct: 856  T--VNGLLWNQLHKITF------------------KKSLTLEKSSSP------------- 882

Query: 2077 TELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAHSI 1898
            +E+   +E+S P +++LF+L+ILE +N   F L + ER  AF+EG + N DD+K     I
Sbjct: 883  SEIGSSVEKSTPCHNLLFLLRILESVNECRFHLMTRERIEAFSEGKIGNLDDVKATNEGI 942

Query: 1897 PQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGV 1718
               EF+N++LT KLEQQM D LA+ T  MP WC QL+ +CPFLFSFEARWKYF L   G 
Sbjct: 943  LVNEFVNTRLTEKLEQQMRDPLAICTGGMPSWCTQLMTSCPFLFSFEARWKYFKLVALG- 1001

Query: 1717 SRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEF 1538
             ++Q QN + +            + +PRKKF V+RN IL SA+KMM  +A +KV+LE+++
Sbjct: 1002 -KHQGQNGQND------------SPMPRKKFLVYRNQILESASKMMDLHANQKVVLEVKY 1048

Query: 1537 SNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFIIVHS--- 1367
              EVGTGLGPTLEF+TLVS+ FQK GMGMWRGD+               SG  IV+S   
Sbjct: 1049 DEEVGTGLGPTLEFFTLVSNEFQKPGMGMWRGDN---------------SGIGIVNSVPF 1093

Query: 1366 GLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEKDL 1187
            GLFP P  N        S + V K F LLGQ+ A+A+ DGR+LD+PFS+AFYKL+L K+L
Sbjct: 1094 GLFPSPLRN--------SITDVNKKFVLLGQVVAKALHDGRVLDIPFSKAFYKLILGKEL 1145

Query: 1186 DIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEELCLDFSLP 1007
             +YDIQSFD  LG+T+IEFQAL +RK  LE K G++S   F+  F  ++I++L LDF+LP
Sbjct: 1146 TVYDIQSFDAGLGKTLIEFQALVERKKNLE-KFGKNS--EFE--FRGSKIQDLHLDFTLP 1200

Query: 1006 GYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIF 827
            GYPDY+ +S  D +MVN+ NLE Y+ LV DAT+ SGI +Q+E+FKSGF++VFPIK L+IF
Sbjct: 1201 GYPDYILASGPDKEMVNMMNLENYIELVADATINSGIMKQMEAFKSGFNQVFPIKNLKIF 1260

Query: 826  TAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQRRAFLLFV 647
            T EELERLLCGE +TW+  +L + I FDHGY+++S P + FLE+IQEFD  Q++AF+ FV
Sbjct: 1261 TDEELERLLCGESETWNSNQLSDLIKFDHGYTASSPPIIHFLEVIQEFDYEQQKAFVKFV 1320

Query: 646  TGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLY 467
            TGAPRLP GGLA+L PKLTIV K CD   D +LPSVMTCANYLKLPPYSSKE M+EKLLY
Sbjct: 1321 TGAPRLPIGGLASLNPKLTIVRKLCDKVVDADLPSVMTCANYLKLPPYSSKEMMKEKLLY 1380

Query: 466  AIREGQGAFHLS 431
            AI EGQG+FHLS
Sbjct: 1381 AITEGQGSFHLS 1392


>gb|PIA45461.1| hypothetical protein AQUCO_01700766v1 [Aquilegia coerulea]
          Length = 1221

 Score = 1004 bits (2597), Expect = 0.0
 Identities = 575/1271 (45%), Positives = 803/1271 (63%), Gaps = 14/1271 (1%)
 Frame = -1

Query: 4201 VLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMC 4022
            +L+++DFF  S QR+ ++ + N+CK++P +    VM+AVP+LCNLL Y+D+KLVE+ A C
Sbjct: 3    ILNYIDFFASSVQRVGITIIANICKKLPSEFSPQVMEAVPLLCNLLYYEDQKLVENVATC 62

Query: 4021 LMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLL 3842
            L+ IV+   HS ++LDE+CK G+ +Q   LI+ +    L++  Y  +I LL RLA+ S+ 
Sbjct: 63   LIWIVKKASHSPDMLDEVCKQGLTQQVAHLIASNVGNILNQPIYTDLIGLLSRLASGSIT 122

Query: 3841 SIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQD 3662
            +  SL E NI++ +  ILR+ D S   PPS   ++        +KLL  L+PP++ +   
Sbjct: 123  ASNSLLEHNISNTMSLILRSYDLSHSKPPSLIHEV--------IKLLYVLLPPLSTNCNG 174

Query: 3661 IQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKNYVLIATPDM 3482
               V   ++II  +P L  QF   +LP L Q V SGAN  I Y C+SV+   +  +T DM
Sbjct: 175  KTEVSNMDRIIAHQPGLLQQFGADVLPVLVQIVNSGANTHICYGCLSVVNKIIHFSTSDM 234

Query: 3481 LRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVL 3302
            L  L+KNAN+  FLAG+++RK+ H+L+ AL +V+ +M++LP    NSF+KEGV + I  L
Sbjct: 235  LVDLLKNANISSFLAGIIARKELHLLMLALTIVKNVMQELPDAFFNSFIKEGVVYTIDAL 294

Query: 3301 SKQDGCYQSQLMDQQSSNL-GDTDERTAANLS-RCLCYAFDXXXXXXXXSKMCNLPRHTL 3128
               + C Q  L   + + L   +++R  AN S  CLCY +D         ++C L   ++
Sbjct: 295  ITPEECPQFILQTSEGAQLLSRSNQRMPANDSVGCLCYVYDIDQSSEN--EICKLEETSV 352

Query: 3127 LPLAQRVMSTFFXXXXXXXXXXXXXLQKL--KACYIALDKNVDSALTNCAEREDYLSQMI 2954
              LA+ + +T+F             L K     C +  DK   S   +  E+E  LS  +
Sbjct: 353  HTLAKYIRTTYFTTKSGCSELKSISLLKKLRNLCAVLSDKENMSVSNDNCEQEKNLSYTL 412

Query: 2953 VEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGDMSFDYLTVL-KRLQTF 2777
             + +  L+  + +STFEFIESG +R L  YL+NG Y +R +D +   ++L VL KR + F
Sbjct: 413  GQILEELSGEEPVSTFEFIESGIVRSLVDYLSNGCYRNRVVDPNELLNHLLVLQKRYEVF 472

Query: 2776 SFKLLS--GHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHP 2603
            +   LS   H  +  PL  L+  LQ+ALS LE FPVVL   +K K     IP + +T HP
Sbjct: 473  AKLCLSPPSHPLKDKPLGILLGKLQSALSLLENFPVVLHDVSKPKETYVTIPFERTTFHP 532

Query: 2602 CLQVHFVTEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXTQRHIK 2426
            CL+V F+ EEG+T LC Y  +V++++  +S +AIE +LWP                +H  
Sbjct: 533  CLKVRFIKEEGDTSLCDYVSDVVSIEAFTSINAIERYLWPRV--------------KHEA 578

Query: 2425 QSSRK---VDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAILQDKINEDPD 2255
            +S+ +   V +++   S+ TQ        KL+F LD  QLD+++TLYQAILQ K+  + D
Sbjct: 579  ESATEECAVATEDLKSSNDTQ--------KLLFYLDKGQLDQTLTLYQAILQLKMETEND 630

Query: 2254 IIVSKKFWNEVHRVTLWS---AKKLQLIYPQISADPRQSTLSWDKTELPWHNNFSFFSSM 2084
            + V   FW +V+ +        KK+ +  P +  +   S   W   ++ +  N   FSSM
Sbjct: 631  LSVGPSFWKQVYEIKYTKDTGQKKINI--PDVHHNSGSSR-EWSHPQI-YLQNAMCFSSM 686

Query: 2083 LLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAH 1904
            L +E P   ++S+  Y++L +LKILE LNR +  L  +ER ++FAEG  ++ D+L V   
Sbjct: 687  LFSEPPLSFKKSSTIYEILTLLKILEVLNRSASHLVYHERISSFAEGCDDSLDNLIVPVC 746

Query: 1903 SIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIF 1724
             +PQTEF+N KLT KLEQQM D   +ST ++P WC+QL+ A PFLF FE R KYF + + 
Sbjct: 747  GVPQTEFVNLKLTGKLEQQMQDASVVSTGSIPSWCVQLMEAFPFLFGFETRCKYFQMTLV 806

Query: 1723 GVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEI 1544
              S  Q  N        I+DR+S ++  PRKKFKV R+ IL SAA+ M  + G+K +LE+
Sbjct: 807  NSSEVQPDN--------IDDRQSQASGSPRKKFKVCRSRILESAAQAMSSHVGRKAILEV 858

Query: 1543 EFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFIIVHSG 1364
            E+  EVGTG GPT+EF+TLVS  FQKVGMGMWRGD   T+    ++  V  SGF++   G
Sbjct: 859  EYPEEVGTGQGPTMEFFTLVSQEFQKVGMGMWRGDPKLTSM---SNSEVDSSGFVVAPFG 915

Query: 1363 LFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEKDLD 1184
            LFPRPWS      N    S+V+K F LLGQL A+A+QDGR+LDLP S + YKL+LE+DL+
Sbjct: 916  LFPRPWS-ASSAPNETQLSEVVKKFVLLGQLVAKALQDGRVLDLPLSCSLYKLILEQDLN 974

Query: 1183 IYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEELCLDFSLPG 1004
            +YDI SFD  +GR ++EFQAL  RK  L+S S    + R D  F NTRIE+LCLDF+LPG
Sbjct: 975  LYDIFSFDHGVGRALVEFQALVDRKKVLDSVS----VKRSDSYFQNTRIEDLCLDFTLPG 1030

Query: 1003 YPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFT 824
            YPDY  +S+   +MVN+SNL+EYVS  +DATV +GI RQ+E+FK GF++V P+K+L+IFT
Sbjct: 1031 YPDYKLTSVDGHEMVNMSNLQEYVSSTLDATVNNGICRQVEAFKLGFNQVLPLKSLKIFT 1090

Query: 823  AEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQRRAFLLFVT 644
            AEEL++LLCGEQ+ W+  EL +HI FDHGY+++S   +K LEI+QEF+  QRRAFL FVT
Sbjct: 1091 AEELDQLLCGEQNAWNYNELLDHIKFDHGYTASSPTIIKLLEIMQEFEYNQRRAFLQFVT 1150

Query: 643  GAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYA 464
            GAPRLPPGGLAAL PKLTIVCK C   AD +LPSVMTCANYLKLPPYSSKE M+E+LLYA
Sbjct: 1151 GAPRLPPGGLAALNPKLTIVCKPCKGGADGDLPSVMTCANYLKLPPYSSKEIMKERLLYA 1210

Query: 463  IREGQGAFHLS 431
            I EGQG+F LS
Sbjct: 1211 ITEGQGSFLLS 1221


>ref|XP_020102558.1| E3 ubiquitin-protein ligase UPL4-like [Ananas comosus]
          Length = 1351

 Score =  949 bits (2453), Expect = 0.0
 Identities = 572/1388 (41%), Positives = 821/1388 (59%), Gaps = 16/1388 (1%)
 Frame = -1

Query: 4546 AYLTAFCDALAFT--QYADDLACLPLDEIVPLLVELLAGRGGS------PDVMLMSARVL 4391
            A L+A CDA A    ++ D LA   +D++   LV+L +GRGG       P V +++AR +
Sbjct: 31   AALSALCDAFAIVPDEFFDSLA---IDDLARHLVDLASGRGGGGEGAVDPSVAVLAARAI 87

Query: 4390 TYFLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVPCLRAGV 4211
            T   D  P +A  A ++ G V ALC  +  + Y+D+AEQCL AL+K+S  +   CL AG+
Sbjct: 88   TLVCDASPDSAG-AFVRRGVVPALCAHVASIKYMDLAEQCLRALEKISLERPEKCLSAGI 146

Query: 4210 INLVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVEST 4031
            +  VL   DFF LS QR+ +S + NV   +  +  S+V++AVP+LCNLL  +++++V+  
Sbjct: 147  VPAVLKHFDFFSLSTQRVVVSIMSNVFSVINLEHSSLVIEAVPVLCNLLQSEEKQIVDGA 206

Query: 4030 AMCLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANR 3851
              CL+ I + F  S +L DE+C  GVV  SV+L++ ++  +L     + +I+LL  LA+ 
Sbjct: 207  LDCLLVIGQVFHTSPKLCDEICMLGVVAHSVQLMNSNNSRSLDRDNLLYLIQLLGFLASG 266

Query: 3850 SLLSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARD 3671
            S++++R L+E NI S+L   L + + S         ++  D +Y  L L+NQLIP V   
Sbjct: 267  SIIAVRILFELNIGSVLSGALTSRNLS-------FHNLELDLIYDVLNLVNQLIPMVETC 319

Query: 3670 DQD-IQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKNYVLIA 3494
            +Q+  QL+  K+K I++      QF   I+    Q V S A   + Y  + ++ N   ++
Sbjct: 320  NQEKTQLLQQKKKTILENHIHFHQFVTNIVFLSIQFVGSNAKLAVSYGFLCMLCNIFSLS 379

Query: 3493 TPDMLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHA 3314
            TP++L  L K  N+   LA L + KDH +L   L + EI+++K P   L SF KEG+ + 
Sbjct: 380  TPELLVELSKTTNISSLLASLSAHKDHQILFHTLKIAEILLEKAPDFHLISFCKEGLVNF 439

Query: 3313 IRVLSKQDGCYQSQLMDQQSSNLGDTDERTAANLSRCLCYAFDXXXXXXXXSKMCNLPRH 3134
            I      D     +   QQS    D D+   AN   C CY+F          + C + + 
Sbjct: 440  I------DAIILRERNPQQSKLRKDKDKSNVAN--SCYCYSFGSDSSPSSDKEACRVGKE 491

Query: 3133 TLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTNCAEREDYLSQM 2957
             +L LA+ + +TFF             + Q LK  +   + N ++  T    ++    + 
Sbjct: 492  AVLALAESIRNTFFSTTSGSKNGGFVRILQNLKDFFSISNNNYNA--TKSLTKDGQTREK 549

Query: 2956 IVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRAL-DGDMSFDYL-TVLKRLQ 2783
            I +A   L++  ++S  EF++SG IR LA YL+NG +L   + D D    +L  V  RL 
Sbjct: 550  ICDA---LHQVKAMSNLEFVDSGIIRSLACYLSNGRFLKGEIKDTDKLLKHLHEVEMRLD 606

Query: 2782 TFSF--KLLSGHSGQHFPLASLVQHLQNALSALEC--FPVVLSAANKSKSRSTDIPAKSS 2615
             F+    L+     +   L+ LV+ LQ+AL A  C  F ++LS   +S+ +   IP ++S
Sbjct: 607  AFACIALLIPDQRSEENILSILVRKLQDALHASCCDNFSLILSDNYQSELKHVTIPLRNS 666

Query: 2614 TKHPCLQVHFVTEEGETDLCLYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXTQR 2435
             +   ++V F     E +L  +D+VL +D++S  +AIE  LWP                +
Sbjct: 667  -EFMKIKVQFRRAVREKNLANFDDVLAIDLNSPLEAIEALLWPHISSNFL---------K 716

Query: 2434 HIKQSSRKVDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAILQDKINEDPD 2255
             +K+S+R   +       T + +E      L F    K+L+RS+TL+++IL++K + + D
Sbjct: 717  GLKKSTRSKTNATAPRELTKETSE------LRFYYKHKRLNRSLTLFESILREKTDAEGD 770

Query: 2254 IIVSKKFWNEVHRVTLWSAKKLQLIYPQISADPRQSTLSWDKTELPWHNNFSFFSSMLLT 2075
            + V+  F++E+H VT  SA+K        S+   Q       + LPW  + SFFSSM++ 
Sbjct: 771  LFVNSSFFDEIHVVTYLSARKA-------SSTSSQKLHYTSGSILPWQKH-SFFSSMVIG 822

Query: 2074 ELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAHSIP 1895
             +P +LE+ +P+YD+LF+LKILEGLNR SFQ+ + ER + FAEG +++FDD+K     +P
Sbjct: 823  RIPCELEQLSPSYDILFILKILEGLNRFSFQISTQERIDEFAEGKIKSFDDIKTSNFLMP 882

Query: 1894 QTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVS 1715
            Q+EF++  L +KLEQQM D+L     T P WC+ L+ A PFLFSF+ RWKYFC+ +    
Sbjct: 883  QSEFVSIMLANKLEQQMKDSLFEKGVT-PSWCIHLMAASPFLFSFDTRWKYFCITLCNAL 941

Query: 1714 RNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFS 1535
            +N+   SE N           S     KK+KV R  IL SA+ +M  +A    L+EIE+ 
Sbjct: 942  QNRLFLSEPN-----------SKEPASKKYKVDREQILQSASLVMASHARSMDLIEIEYH 990

Query: 1534 NEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFIIVHSGLFP 1355
             E GTG GP +EFYT  S   QKVG+G+WRGD +          VV   G++    GLFP
Sbjct: 991  GEAGTGRGPAVEFYTTSSRELQKVGLGLWRGDCSEKG-------VVHADGYVFAPFGLFP 1043

Query: 1354 RPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEKDLDIYD 1175
             PWS     S G+ FS VLK+FFLLGQL ARAI DGRILDLP S+ FYK ML++++D+YD
Sbjct: 1044 HPWSVAGSSSGGIDFSDVLKHFFLLGQLVARAILDGRILDLPLSKPFYKAMLDQEIDMYD 1103

Query: 1174 IQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEELCLDFSLPGYPD 995
            I+SFDPELG+ MIEFQAL KRK +LE+ S E+S   FD  + N  IE+LCL F+LPGY D
Sbjct: 1104 IESFDPELGKIMIEFQALVKRKKYLETNSVENSPHVFDLSYRNLAIEDLCLQFTLPGYCD 1163

Query: 994  YVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEE 815
            Y     G SKMVN+ NLEEYVSLVVDAT  SG+SRQLE+FKSG +KVFPIK L+IFT EE
Sbjct: 1164 YDLKPGGSSKMVNLDNLEEYVSLVVDATTGSGVSRQLEAFKSGINKVFPIKTLKIFTEEE 1223

Query: 814  LERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQRRAFLLFVTGAP 635
            LER+LCGEQD W    L  HI FDHGY++ S  A+  LEI++EF   ++RAFL FVTGAP
Sbjct: 1224 LERILCGEQDAWASCNLVEHIQFDHGYTAVSPAAINLLEIMREFSRDEQRAFLQFVTGAP 1283

Query: 634  RLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIRE 455
            R+P GG ++L PKLT+V K CD DA++ELPSV  C ++LKLPPY++KE+MREKLLYA+ E
Sbjct: 1284 RVPLGGFSSLNPKLTVVQKQCDGDANLELPSVNICHHFLKLPPYTTKEKMREKLLYAMTE 1343

Query: 454  GQGAFHLS 431
            G GA  LS
Sbjct: 1344 GVGALLLS 1351


>gb|PLY83359.1| hypothetical protein LSAT_1X53840 [Lactuca sativa]
          Length = 1342

 Score =  941 bits (2432), Expect = 0.0
 Identities = 573/1392 (41%), Positives = 800/1392 (57%), Gaps = 14/1392 (1%)
 Frame = -1

Query: 4564 DPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVMLMSARVLTY 4385
            + SAQ   L   C+ L+F   +  L+ L  D + P+L++L A    +P++ML+S R LTY
Sbjct: 120  EESAQQEALRELCEILSFCTDSS-LSSLIADSLSPILIKL-ASHESNPEIMLLSIRALTY 177

Query: 4384 FLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVPCLRAGVIN 4205
              D+ P+++   +I+H  V ALC  L+   Y DVAEQCL+AL+K+SR Q + CL++G I 
Sbjct: 178  LCDIHPQSS-ALIIRHDGVTALCKRLLAFEYEDVAEQCLQALEKISREQPLACLQSGAIM 236

Query: 4204 LVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAM 4025
             VL+F+DFF  S QR+ALSTV+N+C ++P +  S  MDAVP+LC LL Y+D +LVE  A 
Sbjct: 237  AVLTFIDFFSTSLQRVALSTVVNICVKLPSEGSSPFMDAVPVLCKLLQYEDNQLVEHVAT 296

Query: 4024 CLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSL 3845
            C                                                  L+++A R  
Sbjct: 297  C--------------------------------------------------LIKIAERVQ 306

Query: 3844 LSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQ 3665
             S   L E + + ++  +    D +  T  SH        + V L   + L+P V+R+ Q
Sbjct: 307  ESSEMLDELSKHGLIHQVAHLIDLNSHTTLSHSVHTGLIGLLVKLASGSMLLPVVSRN-Q 365

Query: 3664 DIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKNYVLIATPD 3485
            ++ L   KE  ++  P L  +F   +LP L Q V SG +  I Y C+SV+   +  +  D
Sbjct: 366  EVPLAAEKEAFLVTHPDLVEKFGNDLLPVLIQVVDSGVDLYICYGCLSVVDKLLYYSNSD 425

Query: 3484 MLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRV 3305
            ML  L+K+ N+ +FLAG+ +RKD HVL+ ALN+ + I++K     +  FVKEGV  AI  
Sbjct: 426  MLLDLLKSTNISRFLAGVFTRKDIHVLMLALNISDTILQKNTDVFMGPFVKEGVLFAIDA 485

Query: 3304 LSKQDGCYQSQLMDQQSSNLGDTDERTAA--NLSRCLCYAFDXXXXXXXXS-KMCNLPRH 3134
            L   + C Q          L +T  +  A  ++ RCLC++FD          + C L   
Sbjct: 486  LIDPEKCSQFMFSMFNDIQLSNTSSKKYAGKDVIRCLCFSFDVNKTSSSSESRKCKLELE 545

Query: 3133 TLLPLAQRVMSTFFXXXXXXXXXXXXXL-QKLKACYIALDKNVDSALTNCAEREDYLSQM 2957
             +  LA+ + + +F             + +KLK+        + S L++   +E Y   +
Sbjct: 546  CVRTLAKHIRTKYFDTNSSTSETGMTPILEKLKS--------LSSELSSMMTQEGY-DHV 596

Query: 2956 IVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGDMSFDYLTVLKRLQTF 2777
            + + ++ LN  D ISTFEF+ESG    L  YL+NGD                   R++ F
Sbjct: 597  LHQIMSILNGRDVISTFEFVESGITDSLVSYLSNGD-----------------CSRVEAF 639

Query: 2776 SFKLLS--GHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHP 2603
                LS       +F L+  +  LQ+ALS++E FPVVL+ + K ++    +P + ST +P
Sbjct: 640  GRLFLSCTNQPLDYFSLSGFISKLQSALSSVEDFPVVLNNSFKHRNSYATVPRRHSTTYP 699

Query: 2602 CLQVHFVTEEGETDLCLYD-NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXTQRHIK 2426
            CL+V FV EEGE+ L  Y  +V TVD  S ++AIE FLWP                    
Sbjct: 700  CLRVLFVKEEGESTLTEYTGDVQTVDPFSDFEAIETFLWPKVCLN--------------N 745

Query: 2425 QSSRKVDSKERVGSSTTQ----NNEGDIEPKLIFLLDGKQLDRSMTLYQAILQDKINEDP 2258
              S    S+  VG S T       +     KL F L+GK++DR +T+YQA+L   I+E  
Sbjct: 746  AQSPSDKSQHDVGQSQTSPQKPTEDASSSQKLSFYLEGKEIDRGLTIYQAVLSQHISEFD 805

Query: 2257 DIIVSKKFWNEVHRVTLWSAKKLQLIYPQISADPRQSTLSWDKTELPWHNNFSFFSSMLL 2078
               V+   WN++H++T                   + +L+ +K+  P             
Sbjct: 806  T--VNGLLWNQLHKITF------------------KKSLTLEKSSSP------------- 832

Query: 2077 TELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAHSI 1898
            +E+   +E+S P +++LF+L+ILE +N   F L + ER  AF+EG + N DD+K     I
Sbjct: 833  SEIGSSVEKSTPCHNLLFLLRILESVNECRFHLMTRERIEAFSEGKIGNLDDVKATNEGI 892

Query: 1897 PQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGV 1718
               EF+N++LT KLEQQM D LA+ T  MP WC QL+ +CPFLFSFEARWKYF L   G 
Sbjct: 893  LVNEFVNTRLTEKLEQQMRDPLAICTGGMPSWCTQLMTSCPFLFSFEARWKYFKLVALG- 951

Query: 1717 SRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEF 1538
             ++Q QN + +            + +PRKKF V+RN IL SA+KMM  +A +KV+LE+++
Sbjct: 952  -KHQGQNGQND------------SPMPRKKFLVYRNQILESASKMMDLHANQKVVLEVKY 998

Query: 1537 SNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLGTSMVVSDSGFIIVHS--- 1367
              EVGTGLGPTLEF+TLVS+ FQK GMGMWRGD+               SG  IV+S   
Sbjct: 999  DEEVGTGLGPTLEFFTLVSNEFQKPGMGMWRGDN---------------SGIGIVNSVPF 1043

Query: 1366 GLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEKDL 1187
            GLFP P  N        S + V K F LLGQ+ A+A+ DGR+LD+PFS+AFYKL+L K+L
Sbjct: 1044 GLFPSPLRN--------SITDVNKKFVLLGQVVAKALHDGRVLDIPFSKAFYKLILGKEL 1095

Query: 1186 DIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFHNTRIEELCLDFSLP 1007
             +YDIQSFD  LG+T+IEFQAL +RK  LE K G++S   F+  F  ++I++L LDF+LP
Sbjct: 1096 TVYDIQSFDAGLGKTLIEFQALVERKKNLE-KFGKNS--EFE--FRGSKIQDLHLDFTLP 1150

Query: 1006 GYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIF 827
            GYPDY+ +S  D +MVN+ NLE Y+ LV DAT+ SGI +Q+E+FKSGF++VFPIK L+IF
Sbjct: 1151 GYPDYILASGPDKEMVNMMNLENYIELVADATINSGIMKQMEAFKSGFNQVFPIKNLKIF 1210

Query: 826  TAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQRRAFLLFV 647
            T EELERLLCGE +TW+  +L + I FDHGY+++S P + FLE+IQEFD  Q++AF+ FV
Sbjct: 1211 TDEELERLLCGESETWNSNQLSDLIKFDHGYTASSPPIIHFLEVIQEFDYEQQKAFVKFV 1270

Query: 646  TGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLY 467
            TGAPRLP GGLA+L PKLTIV K CD   D +LPSVMTCANYLKLPPYSSKE M+EKLLY
Sbjct: 1271 TGAPRLPIGGLASLNPKLTIVRKLCDKVVDADLPSVMTCANYLKLPPYSSKEMMKEKLLY 1330

Query: 466  AIREGQGAFHLS 431
            AI EGQG+FHLS
Sbjct: 1331 AITEGQGSFHLS 1342


>gb|PNS98591.1| hypothetical protein POPTR_016G085200v3 [Populus trichocarpa]
          Length = 1427

 Score =  880 bits (2273), Expect = 0.0
 Identities = 517/1179 (43%), Positives = 718/1179 (60%), Gaps = 25/1179 (2%)
 Frame = -1

Query: 4564 DPSAQIAYLTAFCDALAFTQYADDLACLPLDEIVPLLVELLAGRGGSPDVMLMSARVLTY 4385
            +PS Q+A LT  C+ L+F    D L+    D + P+LV L +    +PD+ML++ R LTY
Sbjct: 117  EPSGQLAALTELCEVLSFCT-EDSLSSTMADLLSPVLVRL-SRHDSNPDIMLLAIRALTY 174

Query: 4384 FLDLMPRAADRALIKHGAVEALCGPLMEVVYLDVAEQCLEALDKLSRTQSVPCLRAGVIN 4205
              D+ PRA+   L++H A+ A+C  LM + YLDVAEQCL+AL+K++R Q +PCL+AG I 
Sbjct: 175  LCDVFPRASV-FLVRHDAIPAICQRLMAIEYLDVAEQCLQALEKITRDQPLPCLQAGAIM 233

Query: 4204 LVLSFMDFFELSAQRIALSTVINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAM 4025
             VLSF+DFF  S QR+ALSTV+N+CK++P + FS  M+AVP+LCNLL Y+DR+LVE+ A+
Sbjct: 234  AVLSFIDFFSTSVQRVALSTVVNICKKLPSENFSPFMEAVPILCNLLQYEDRQLVENVAI 293

Query: 4024 CLMRIVENFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSL 3845
            CL++I E    SSE+LDELCKHG++ Q+  L+  +S   LS+  Y G+I LLV+L++ S+
Sbjct: 294  CLIKIAERVSQSSEMLDELCKHGLINQATHLVQLNSRTTLSQPVYNGLIGLLVKLSSGSI 353

Query: 3844 LSIRSLYESNINSILESILRNPDHSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQ 3665
            ++ R+LYE NI+SIL+ +    D S      H  D   +QV+  LKLLN+L+P VAR+  
Sbjct: 354  VAFRTLYELNISSILKDLFATYDLSHGISSPHVIDGQGNQVHEVLKLLNELLPTVARNQD 413

Query: 3664 DIQLVLGKEKIIIDEPKLSFQFAVVILPALFQAVTSGANACIHYACVSVIKNYVLIATPD 3485
              QLVL KE  + + P L  +F   I+P+L Q V SGAN  + Y C+ VI   V ++  D
Sbjct: 414  AQQLVLDKEAFLANHPDLLHKFGSDIIPSLIQVVNSGANLYVCYGCLYVINKLVYLSKSD 473

Query: 3484 MLRALIKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRV 3305
            ML  L+KN N   FLAG+L+RKDHHVL+ AL + E I++KLP   +NSF+KEGV  AI  
Sbjct: 474  MLLELLKNTNFSSFLAGVLTRKDHHVLMLALQITETILQKLPDVFVNSFIKEGVFFAIDG 533

Query: 3304 LSKQDGCYQSQLMDQQSSN----LGDTDERTAANLSRCLCYAFDXXXXXXXXSK-MCNLP 3140
            L   + C  SQL+    +     L    + ++  + RCLCYAFD            C L 
Sbjct: 534  LLVPEKC--SQLIFPACNGIHLPLNSNQKSSSKVVMRCLCYAFDTGQSLSASETGTCKLE 591

Query: 3139 RHTLLPLAQRV-MSTFFXXXXXXXXXXXXXLQKLKACYIALD--KNVDSALTNCAEREDY 2969
            + T+  L + + +S F              LQKL+A    L    N+   + +C + E+ 
Sbjct: 592  KDTVENLGKHIRISYFALESCDSEKGLTDILQKLRALSAELSDLMNMSVKIGSCTQDEEK 651

Query: 2968 LSQMIVEAVTALNEGDSISTFEFIESGFIRFLAHYLTNGDYLHRALDGDMSF-DYLTVLK 2792
               ++ + +  L+  + +STFEFIESG ++ L +YL NG YL   ++   +F D+  V K
Sbjct: 652  CYSILCQIMEKLDGREPVSTFEFIESGIVKILVNYLFNGKYLREKVEPQSTFDDFYVVEK 711

Query: 2791 RLQTFSFKLLSGHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSST 2612
            R + F+  L S    +  PL++L+Q LQ ALS+ E FPV+LS A+K +S    IP    T
Sbjct: 712  RFEVFARLLSSSDLSEESPLSALIQKLQGALSSSENFPVILSHASKYRSSFAIIPNGRRT 771

Query: 2611 KHPCLQVHFVTEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXTQ- 2438
             +PCL+V FV  EGET LC Y ++ +TVD  SS + IE FL P                 
Sbjct: 772  SYPCLRVRFVRGEGETCLCNYSEDPVTVDPLSSVNTIEGFLSPKVRIKGTEQIESAAQAL 831

Query: 2437 --------RHIKQSSRKVDSKERVGSSTTQNNEGDIEPKLIFLLDGKQLDRSMTLYQAIL 2282
                      I QS    D   +    T+ +N GD  PKL+F L+G+QLDR++TLYQAIL
Sbjct: 832  EPAENVSVEDIVQSPSCADDSTKSHCPTSCSN-GDAMPKLVFYLEGQQLDRTLTLYQAIL 890

Query: 2281 QDKINEDPDIIVSKKFWNEVHRVTLWSAKKLQLIYPQISADPRQSTLSWDKTELPWHNNF 2102
            Q K+  D +I  + K W +VH +T   A   +    Q      Q++   D+  + +  + 
Sbjct: 891  QQKVKADHEINSTAKLWTQVHTLTYRIAVDTRDDNTQDCPSMAQNSSILDQA-VAFMQHP 949

Query: 2101 SFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDD 1922
            +FFSSM   ELP  L++S+P  D+LF+LK LEGLNR  F L S+ER +AFAEG ++N D+
Sbjct: 950  AFFSSMFNCELPSDLDKSSPTNDILFLLKSLEGLNRFIFHLMSHERIHAFAEGLIDNLDN 1009

Query: 1921 LKVIAHSIPQTEFINSKLTSKLEQQMHDNLALSTATMPLWCMQLVVACPFLFSFEARWKY 1742
            L+V A  + Q EF++SKLT KLEQQM D+LA+S   MP+WC QL+ +C FLFSFE R KY
Sbjct: 1010 LRVAARPVAQNEFVSSKLTEKLEQQMRDSLAVSMGGMPVWCNQLMNSCSFLFSFETRCKY 1069

Query: 1741 FCLNIFGVSRNQTQNSEKNEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGK 1562
            F L+ FG  + Q Q S  N    + DR   + SL RKKF V R+ +L SAA+MM  YA  
Sbjct: 1070 FQLSAFGCQQIQIQPSSHNNSGVLRDRLPSAGSLSRKKFIVLRDQVLESAAQMMDRYAHL 1129

Query: 1561 KVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTS--TAKRLGTSMVVSDS 1388
            KV +E+ ++ EVGTGLGPTLEFYTLVS  FQK G+GMWR DH S  T + L        S
Sbjct: 1130 KVPIEVVYNEEVGTGLGPTLEFYTLVSKEFQKSGIGMWREDHISFPTIENLQAEY----S 1185

Query: 1387 GFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYK 1208
            G +    GLFPRPWS  +D S+GV FS+V+K FFLLGQ+ A+A+QDGR+LDLPF++ FYK
Sbjct: 1186 GIVKSPFGLFPRPWSPTVDASDGVQFSEVIKKFFLLGQIVAKALQDGRVLDLPFAKVFYK 1245

Query: 1207 LMLEK----DLDIYDIQSFDPELGRTMIEFQALAKRKGF 1103
            L+L++    +L++Y     D  +  T I  Q  A + GF
Sbjct: 1246 LILQQVNMDNLEVYVSHIVDATI-HTGISRQVEAFKSGF 1283



 Score =  261 bits (667), Expect = 7e-67
 Identities = 126/179 (70%), Positives = 151/179 (84%)
 Frame = -1

Query: 967  KMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQ 788
            + VN+ NLE YVS +VDAT+ +GISRQ+E+FKSGF++VFPIK L IFT EELERLLCGE+
Sbjct: 1249 QQVNMDNLEVYVSHIVDATIHTGISRQVEAFKSGFNQVFPIKHLMIFTEEELERLLCGER 1308

Query: 787  DTWDIIELQNHINFDHGYSSTSHPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAA 608
            D W   EL +HI FDHGY+++S P +  LEII+EF+  QRR+FL FVTGAPRLP GGLA+
Sbjct: 1309 DFWAFNELLDHIKFDHGYTASSPPIVNLLEIIKEFEYEQRRSFLQFVTGAPRLPTGGLAS 1368

Query: 607  LKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 431
            L PKLTIV KHC N  D++LPSVMTCANYLKLPPYSSK++M+EKLLYAI EGQG+FHLS
Sbjct: 1369 LNPKLTIVRKHCSNCEDVDLPSVMTCANYLKLPPYSSKDKMKEKLLYAITEGQGSFHLS 1427