BLASTX nr result
ID: Ophiopogon25_contig00001402
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon25_contig00001402
(720 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ONK67481.1| uncharacterized protein A4U43_C05F500 [Asparagus ... 168 2e-49
gb|PIA53212.1| hypothetical protein AQUCO_00900061v1 [Aquilegia ... 165 4e-48
ref|XP_020268277.1| cysteine proteinase inhibitor 12-like isofor... 166 6e-48
gb|PIA53214.1| hypothetical protein AQUCO_00900061v1 [Aquilegia ... 162 5e-47
ref|XP_020273952.1| cysteine proteinase inhibitor 12-like, parti... 164 7e-47
gb|AAL79831.1|AF469485_1 cystatin [Sandersonia aurantiaca] 159 2e-46
ref|XP_013749939.1| cysteine proteinase inhibitor 6 [Brassica na... 163 3e-46
gb|ONK63669.1| uncharacterized protein A4U43_C07F17670 [Asparagu... 164 3e-46
ref|NP_001288858.1| cysteine proteinase inhibitor 6-like [Brassi... 161 4e-46
ref|XP_020268275.1| cysteine proteinase inhibitor 12-like isofor... 162 4e-46
gb|PIA53210.1| hypothetical protein AQUCO_00900061v1 [Aquilegia ... 162 8e-46
emb|CDY42150.1| BnaC05g40690D [Brassica napus] 159 2e-45
gb|PIA53209.1| hypothetical protein AQUCO_00900061v1 [Aquilegia ... 159 3e-45
ref|XP_013731755.1| cysteine proteinase inhibitor 6-like [Brassi... 159 5e-45
emb|CDY08429.1| BnaA05g26650D [Brassica napus] 159 5e-45
ref|XP_013584016.1| PREDICTED: cysteine proteinase inhibitor 6-l... 159 6e-45
gb|PIA53211.1| hypothetical protein AQUCO_00900061v1 [Aquilegia ... 159 8e-45
ref|XP_010909027.1| PREDICTED: cysteine proteinase inhibitor 6 [... 159 8e-45
gb|AAC37479.1| cysteine proteinase inhibitor [Brassica rapa subs... 158 8e-45
ref|XP_013646830.1| cysteine proteinase inhibitor 6-like [Brassi... 159 9e-45
>gb|ONK67481.1| uncharacterized protein A4U43_C05F500 [Asparagus officinalis]
Length = 141
Score = 168 bits (425), Expect = 2e-49
Identities = 83/107 (77%), Positives = 93/107 (86%)
Frame = +1
Query: 130 GGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLEAI 309
GGIQD + EN E+EELGRFAVDEHNK+QNTLLEFAGVV AKEQ+VAGT++YLT+EAI
Sbjct: 36 GGIQDRRMG-ENDAEMEELGRFAVDEHNKQQNTLLEFAGVVNAKEQVVAGTMYYLTVEAI 94
Query: 310 EAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRGD 450
E GKKKLYEAKVWVKPWLNFKELQEF P+ DSS+ T ADLGAK+ D
Sbjct: 95 EGGKKKLYEAKVWVKPWLNFKELQEFTPLADSSSGATAADLGAKQDD 141
>gb|PIA53212.1| hypothetical protein AQUCO_00900061v1 [Aquilegia coerulea]
Length = 152
Score = 165 bits (417), Expect = 4e-48
Identities = 79/110 (71%), Positives = 93/110 (84%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
+ GG+ D E +NS+EIEEL +FAVDEHNKK+N LLEFA VVKAKEQ+VAGT+H+LTLE
Sbjct: 44 VLGGVTDSE-STQNSVEIEELAKFAVDEHNKKENALLEFARVVKAKEQVVAGTMHHLTLE 102
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRGDC 453
AIEAGKKK+YEAKVWVKPW+NFKEL EF GDS+ VTP+DLG K+G C
Sbjct: 103 AIEAGKKKIYEAKVWVKPWMNFKELTEFKHTGDSTPDVTPSDLGVKQGGC 152
>ref|XP_020268277.1| cysteine proteinase inhibitor 12-like isoform X2 [Asparagus
officinalis]
Length = 213
Score = 166 bits (421), Expect = 6e-48
Identities = 82/107 (76%), Positives = 93/107 (86%)
Frame = +1
Query: 130 GGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLEAI 309
GGIQD + EN E+EELGRFAVDEHNK+QNTLLEFAGVV AKEQ+VAGT++YLT+EAI
Sbjct: 17 GGIQDRRMG-ENDAEMEELGRFAVDEHNKQQNTLLEFAGVVNAKEQVVAGTMYYLTVEAI 75
Query: 310 EAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRGD 450
E GKKKLYEAKVWVKPWLNFKELQEF P+ DSS+ T ADLGAK+ +
Sbjct: 76 EGGKKKLYEAKVWVKPWLNFKELQEFTPLADSSSGATAADLGAKQDE 122
>gb|PIA53214.1| hypothetical protein AQUCO_00900061v1 [Aquilegia coerulea]
Length = 153
Score = 162 bits (410), Expect = 5e-47
Identities = 78/109 (71%), Positives = 93/109 (85%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
+ GG+ D E +NS+EIEEL +FAVDEHNKK+N LLEFA VVKAKEQ+VAGT+H+LTLE
Sbjct: 44 VLGGVTDSE-STQNSVEIEELAKFAVDEHNKKENALLEFARVVKAKEQVVAGTMHHLTLE 102
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRGD 450
AIEAGKKK+YEAKVWVKPW+NFKEL EF GDS+ VTP+DLG K+G+
Sbjct: 103 AIEAGKKKIYEAKVWVKPWMNFKELTEFKHTGDSTPDVTPSDLGVKQGN 151
>ref|XP_020273952.1| cysteine proteinase inhibitor 12-like, partial [Asparagus
officinalis]
Length = 222
Score = 164 bits (415), Expect = 7e-47
Identities = 81/108 (75%), Positives = 91/108 (84%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
I GGI + ENS EIEEL RFAVDEHNKKQN LLEF+ V+KAKEQ VAG +HYLT+E
Sbjct: 24 ILGGIHQRSVG-ENSAEIEELARFAVDEHNKKQNALLEFSRVLKAKEQAVAGIMHYLTVE 82
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRG 447
AIEAGKKK+YEAK+WVKPWL+FKELQEF GDSS ++TPADLGAKRG
Sbjct: 83 AIEAGKKKIYEAKIWVKPWLDFKELQEFTHTGDSSPTITPADLGAKRG 130
>gb|AAL79831.1|AF469485_1 cystatin [Sandersonia aurantiaca]
Length = 110
Score = 159 bits (402), Expect = 2e-46
Identities = 80/107 (74%), Positives = 89/107 (83%)
Frame = +1
Query: 130 GGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLEAI 309
G +D+ ENS ++EEL RFAV EHNKK+N LLEF VVKAKEQ+VAGTLH+LT+EAI
Sbjct: 5 GAPRDVPAGGENSADVEELARFAVAEHNKKENALLEFGRVVKAKEQVVAGTLHHLTVEAI 64
Query: 310 EAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRGD 450
+AG KKLYEAKVWVKPWLNFKELQEF GD S SVTPADLGAKRGD
Sbjct: 65 DAGNKKLYEAKVWVKPWLNFKELQEFRHAGD-SPSVTPADLGAKRGD 110
>ref|XP_013749939.1| cysteine proteinase inhibitor 6 [Brassica napus]
Length = 234
Score = 163 bits (412), Expect = 3e-46
Identities = 76/109 (69%), Positives = 94/109 (86%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
+ GG++D+ + +NS E+E L RFAVDEHNKK+N LLEFA VVKAKEQ+VAGT+H+LTLE
Sbjct: 35 LLGGVRDVPAN-QNSDEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTMHHLTLE 93
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRGD 450
+EAGKKKLYEAKVWVKPWLNFKELQEF P D + S+TP+DLG+K+G+
Sbjct: 94 IVEAGKKKLYEAKVWVKPWLNFKELQEFKPASDGAPSITPSDLGSKKGE 142
>gb|ONK63669.1| uncharacterized protein A4U43_C07F17670 [Asparagus officinalis]
Length = 271
Score = 164 bits (415), Expect = 3e-46
Identities = 81/108 (75%), Positives = 91/108 (84%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
I GGI + ENS EIEEL RFAVDEHNKKQN LLEF+ V+KAKEQ VAG +HYLT+E
Sbjct: 73 ILGGIHQRSVG-ENSAEIEELARFAVDEHNKKQNALLEFSRVLKAKEQAVAGIMHYLTVE 131
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRG 447
AIEAGKKK+YEAK+WVKPWL+FKELQEF GDSS ++TPADLGAKRG
Sbjct: 132 AIEAGKKKIYEAKIWVKPWLDFKELQEFTHTGDSSPTITPADLGAKRG 179
>ref|NP_001288858.1| cysteine proteinase inhibitor 6-like [Brassica rapa]
gb|ABK78689.1| cysteine proteinase inhibitor [Brassica rapa]
Length = 202
Score = 161 bits (408), Expect = 4e-46
Identities = 76/109 (69%), Positives = 92/109 (84%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
+ GG++D+ +NS E+E L RFAVDEHNKK+N LLEFA VVKAKEQ+VAGT+H+LTLE
Sbjct: 3 LLGGVRDVPA-YQNSDEVESLARFAVDEHNKKENVLLEFARVVKAKEQVVAGTMHHLTLE 61
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRGD 450
+EAGKKKLYEAKVWVKPWLNFKELQEF P D + S+TP+DLG K+G+
Sbjct: 62 IVEAGKKKLYEAKVWVKPWLNFKELQEFKPASDGAPSITPSDLGCKKGE 110
>ref|XP_020268275.1| cysteine proteinase inhibitor 12-like isoform X1 [Asparagus
officinalis]
Length = 239
Score = 162 bits (411), Expect = 4e-46
Identities = 78/97 (80%), Positives = 87/97 (89%)
Frame = +1
Query: 160 ENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLEAIEAGKKKLYEA 339
ENS EIE+L RFAVDEHNKKQNTLLEFAGVV AKEQ+VAGT++YLT+EAIE GKKKLYEA
Sbjct: 52 ENSAEIEDLARFAVDEHNKKQNTLLEFAGVVNAKEQVVAGTMYYLTVEAIEGGKKKLYEA 111
Query: 340 KVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRGD 450
KVWVKPWLNFKELQEF P+ DSS+ T ADLGAK+ +
Sbjct: 112 KVWVKPWLNFKELQEFTPLADSSSGATAADLGAKQDE 148
>gb|PIA53210.1| hypothetical protein AQUCO_00900061v1 [Aquilegia coerulea]
Length = 238
Score = 162 bits (409), Expect = 8e-46
Identities = 78/108 (72%), Positives = 92/108 (85%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
+ GG+ D E +NS+EIEEL +FAVDEHNKK+N LLEFA VVKAKEQ+VAGT+H+LTLE
Sbjct: 44 VLGGVTDSE-STQNSVEIEELAKFAVDEHNKKENALLEFARVVKAKEQVVAGTMHHLTLE 102
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRG 447
AIEAGKKK+YEAKVWVKPW+NFKEL EF GDS+ VTP+DLG K+G
Sbjct: 103 AIEAGKKKIYEAKVWVKPWMNFKELTEFKHTGDSTPDVTPSDLGVKQG 150
>emb|CDY42150.1| BnaC05g40690D [Brassica napus]
Length = 203
Score = 159 bits (403), Expect = 2e-45
Identities = 76/110 (69%), Positives = 96/110 (87%), Gaps = 1/110 (0%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
+ GG++D+ + +NS E+E L RFAVDEHNKK+N+LLEFA VVKAKEQ+VAGT+H+LTLE
Sbjct: 3 LLGGVRDVPAN-QNSGEVESLARFAVDEHNKKENSLLEFARVVKAKEQVVAGTMHHLTLE 61
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSS-ASVTPADLGAKRGD 450
+EAGKKKLYEAKVWVKPWLNFKELQEF P D++ +S+TP+DLG K+G+
Sbjct: 62 IVEAGKKKLYEAKVWVKPWLNFKELQEFKPASDAAPSSITPSDLGCKKGE 111
>gb|PIA53209.1| hypothetical protein AQUCO_00900061v1 [Aquilegia coerulea]
gb|PIA53213.1| hypothetical protein AQUCO_00900061v1 [Aquilegia coerulea]
Length = 206
Score = 159 bits (403), Expect = 3e-45
Identities = 77/107 (71%), Positives = 91/107 (85%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
+ GG+ D E +NS+EIEEL +FAVDEHNKK+N LLEFA VVKAKEQ+VAGT+H+LTLE
Sbjct: 44 VLGGVTDSE-STQNSVEIEELAKFAVDEHNKKENALLEFARVVKAKEQVVAGTMHHLTLE 102
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKR 444
AIEAGKKK+YEAKVWVKPW+NFKEL EF GDS+ VTP+DLG K+
Sbjct: 103 AIEAGKKKIYEAKVWVKPWMNFKELTEFKHTGDSTPDVTPSDLGVKQ 149
>ref|XP_013731755.1| cysteine proteinase inhibitor 6-like [Brassica napus]
Length = 229
Score = 159 bits (403), Expect = 5e-45
Identities = 76/110 (69%), Positives = 96/110 (87%), Gaps = 1/110 (0%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
+ GG++D+ + +NS E+E L RFAVDEHNKK+N+LLEFA VVKAKEQ+VAGT+H+LTLE
Sbjct: 29 LLGGVRDVPAN-QNSGEVESLARFAVDEHNKKENSLLEFARVVKAKEQVVAGTMHHLTLE 87
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSS-ASVTPADLGAKRGD 450
+EAGKKKLYEAKVWVKPWLNFKELQEF P D++ +S+TP+DLG K+G+
Sbjct: 88 IVEAGKKKLYEAKVWVKPWLNFKELQEFKPASDAAPSSITPSDLGCKKGE 137
>emb|CDY08429.1| BnaA05g26650D [Brassica napus]
Length = 229
Score = 159 bits (403), Expect = 5e-45
Identities = 76/110 (69%), Positives = 96/110 (87%), Gaps = 1/110 (0%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
+ GG++D+ + +NS E+E L RFAVDEHNKK+N+LLEFA VVKAKEQ+VAGT+H+LTLE
Sbjct: 29 LLGGVRDVPAN-QNSGEVESLARFAVDEHNKKENSLLEFARVVKAKEQVVAGTMHHLTLE 87
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSS-ASVTPADLGAKRGD 450
+EAGKKKLYEAKVWVKPWLNFKELQEF P D++ +S+TP+DLG K+G+
Sbjct: 88 IVEAGKKKLYEAKVWVKPWLNFKELQEFKPASDAAPSSITPSDLGCKKGE 137
>ref|XP_013584016.1| PREDICTED: cysteine proteinase inhibitor 6-like [Brassica oleracea
var. oleracea]
Length = 232
Score = 159 bits (403), Expect = 6e-45
Identities = 76/110 (69%), Positives = 96/110 (87%), Gaps = 1/110 (0%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
+ GG++D+ + +NS E+E L RFAVDEHNKK+N+LLEFA VVKAKEQ+VAGT+H+LTLE
Sbjct: 32 LLGGVRDVPAN-QNSGEVESLARFAVDEHNKKENSLLEFARVVKAKEQVVAGTMHHLTLE 90
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSS-ASVTPADLGAKRGD 450
+EAGKKKLYEAKVWVKPWLNFKELQEF P D++ +S+TP+DLG K+G+
Sbjct: 91 IVEAGKKKLYEAKVWVKPWLNFKELQEFKPASDAAPSSITPSDLGCKKGE 140
>gb|PIA53211.1| hypothetical protein AQUCO_00900061v1 [Aquilegia coerulea]
Length = 243
Score = 159 bits (403), Expect = 8e-45
Identities = 77/107 (71%), Positives = 91/107 (85%)
Frame = +1
Query: 124 ICGGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLE 303
+ GG+ D E +NS+EIEEL +FAVDEHNKK+N LLEFA VVKAKEQ+VAGT+H+LTLE
Sbjct: 44 VLGGVTDSE-STQNSVEIEELAKFAVDEHNKKENALLEFARVVKAKEQVVAGTMHHLTLE 102
Query: 304 AIEAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKR 444
AIEAGKKK+YEAKVWVKPW+NFKEL EF GDS+ VTP+DLG K+
Sbjct: 103 AIEAGKKKIYEAKVWVKPWMNFKELTEFKHTGDSTPDVTPSDLGVKQ 149
>ref|XP_010909027.1| PREDICTED: cysteine proteinase inhibitor 6 [Elaeis guineensis]
Length = 243
Score = 159 bits (403), Expect = 8e-45
Identities = 82/106 (77%), Positives = 93/106 (87%)
Frame = +1
Query: 130 GGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLEAI 309
G ++D +I+ ENS+EIEELGRFAV EHNKK+N LLEFA VVKAKEQ+VAGTLH+LTLE +
Sbjct: 44 GDVRDSQIN-ENSVEIEELGRFAVQEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEVM 102
Query: 310 EAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRG 447
EAGKKKL+EAKVWVKPWLNFKELQEF +GD S SVT ADLGAK G
Sbjct: 103 EAGKKKLFEAKVWVKPWLNFKELQEFTHLGD-SPSVTAADLGAKHG 147
>gb|AAC37479.1| cysteine proteinase inhibitor [Brassica rapa subsp. oleifera]
Length = 199
Score = 158 bits (399), Expect = 8e-45
Identities = 77/107 (71%), Positives = 91/107 (85%)
Frame = +1
Query: 130 GGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLEAI 309
GG++D+ ENS+E+E L RFAVDEHNKK+N LLEFA VVKAKEQ+VAGT+H+LTLE I
Sbjct: 5 GGVRDVP-SNENSVEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTMHHLTLEII 63
Query: 310 EAGKKKLYEAKVWVKPWLNFKELQEFAPVGDSSASVTPADLGAKRGD 450
EAGKKKLYEAKVWVKPWLNFKELQEF P S ++TP+DLG K+G+
Sbjct: 64 EAGKKKLYEAKVWVKPWLNFKELQEFKP----STTITPSDLGCKKGE 106
>ref|XP_013646830.1| cysteine proteinase inhibitor 6-like [Brassica napus]
Length = 235
Score = 159 bits (402), Expect = 9e-45
Identities = 78/110 (70%), Positives = 93/110 (84%), Gaps = 3/110 (2%)
Frame = +1
Query: 130 GGIQDIEIDRENSLEIEELGRFAVDEHNKKQNTLLEFAGVVKAKEQLVAGTLHYLTLEAI 309
GG++D+ + +NS E+E L RFAVDEHNKK+N LLEFA VVKAKEQ+VAGT+H+LTLE I
Sbjct: 35 GGVRDVPAN-QNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTMHHLTLEII 93
Query: 310 EAGKKKLYEAKVWVKPWLNFKELQEFAPVGDS---SASVTPADLGAKRGD 450
EAGKKKLYEAKVWVKPWLNFKELQEF P D SA++TP+DLG K+G+
Sbjct: 94 EAGKKKLYEAKVWVKPWLNFKELQEFKPASDDGSPSATITPSDLGCKKGE 143