BLASTX nr result
ID: Ophiopogon24_contig00030943
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon24_contig00030943
(764 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_020273972.1| isoamylase 2, chloroplastic [Asparagus offic... 286 2e-87
ref|XP_008799133.1| PREDICTED: isoamylase 2, chloroplastic [Phoe... 277 4e-84
ref|XP_010278620.1| PREDICTED: isoamylase 2, chloroplastic [Nelu... 271 1e-81
ref|XP_020700621.1| isoamylase 2, chloroplastic [Dendrobium cate... 265 2e-79
gb|PIA39269.1| hypothetical protein AQUCO_02600010v1 [Aquilegia ... 258 8e-77
ref|XP_010938750.1| PREDICTED: isoamylase 2, chloroplastic [Elae... 254 3e-75
gb|PKA66852.1| Isoamylase 2, chloroplastic [Apostasia shenzhenica] 254 4e-75
ref|XP_020087102.1| isoamylase 2, chloroplastic [Ananas comosus]... 253 5e-75
gb|OAY83378.1| Isoamylase 2, chloroplastic [Ananas comosus] 253 9e-74
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti... 246 3e-72
ref|XP_020592870.1| isoamylase 2, chloroplastic [Phalaenopsis eq... 243 2e-71
gb|AOQ26242.1| ISA2 [Actinidia deliciosa] 239 1e-69
ref|XP_020524299.1| isoamylase 2, chloroplastic isoform X5 [Ambo... 236 1e-68
ref|XP_020524298.1| isoamylase 2, chloroplastic isoform X4 [Ambo... 236 2e-68
ref|XP_011624242.1| isoamylase 2, chloroplastic isoform X2 [Ambo... 236 2e-68
gb|ERN08315.1| hypothetical protein AMTR_s00156p00067780 [Ambore... 236 4e-68
ref|XP_020524296.1| isoamylase 2, chloroplastic isoform X1 [Ambo... 236 4e-68
ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber] ... 232 5e-67
ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium] 228 2e-65
ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]... 227 3e-65
>ref|XP_020273972.1| isoamylase 2, chloroplastic [Asparagus officinalis]
ref|XP_020273973.1| isoamylase 2, chloroplastic [Asparagus officinalis]
gb|ONK62840.1| uncharacterized protein A4U43_C07F8670 [Asparagus officinalis]
Length = 855
Score = 286 bits (732), Expect = 2e-87
Identities = 149/254 (58%), Positives = 174/254 (68%)
Frame = -1
Query: 764 GLGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSW 585
G LNYNAE QR SYLFRTE+GGLVKVLVG NNMKY V I+VSSLP N DLILSW
Sbjct: 69 GSDLNYNAETGQRATSYLFRTEIGGLVKVLVGFNNMKYAVRIDVSSLPTRTN--DLILSW 126
Query: 584 GMFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXX 405
G+ RSDS+ L AP +TP QN G ++TLEF S Q+P
Sbjct: 127 GVHRSDSNQLDAP------------------GCTKTPLVQNLKGRFSVTLEFSSTQIPFY 168
Query: 404 XXXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAE 225
+SGI+THR TNFC+PVG+S+G P+PLG S+ E GLVNFALFSR AE
Sbjct: 169 LSFLLHSSSDHPRCESGIRTHRMTNFCLPVGMSAGHPMPLGVSRCEGGLVNFALFSRNAE 228
Query: 224 SVNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRF 45
SV L L+++ N+P L++ELDPY+NRTGDIWH+SME+VG+Y SYGYRCKGE D GSRF
Sbjct: 229 SVVLRLFDNNVNEPCLELELDPYVNRTGDIWHLSMENVGSYTSYGYRCKGETGSDKGSRF 288
Query: 44 HASHVLLDPYAKLI 3
HA VLLDPYAKLI
Sbjct: 289 HARQVLLDPYAKLI 302
>ref|XP_008799133.1| PREDICTED: isoamylase 2, chloroplastic [Phoenix dactylifera]
Length = 853
Score = 277 bits (709), Expect = 4e-84
Identities = 138/253 (54%), Positives = 175/253 (69%)
Frame = -1
Query: 761 LGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWG 582
LG++ + ++LQ+ FSY+FRTE+GGLVKVLVG +M+Y V +EVSSLP + E L+++WG
Sbjct: 68 LGVSDSTQELQKPFSYMFRTEIGGLVKVLVGTKSMRYAVRVEVSSLPRAVGEDGLVMNWG 127
Query: 581 MFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXX 402
MFRSDSS L A DSQ + F +NSLG + + LEFDSA++P
Sbjct: 128 MFRSDSSQLLAQDSQVA-------------------FVRNSLGGYMVELEFDSARIPFYL 168
Query: 401 XXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAES 222
S I+THRKT FCVPVG+ G P+PLG S S+DG VNF+LFSR AE
Sbjct: 169 SFLLSSPPSV----SEIRTHRKTKFCVPVGLGPGWPMPLGVSISDDGAVNFSLFSRNAEG 224
Query: 221 VNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFH 42
V LCL++ +PSL+++LDPY+NRTGDIWHVSMES+GN+VSYGYRCKG WD RFH
Sbjct: 225 VVLCLFDGKTREPSLEMDLDPYVNRTGDIWHVSMESMGNFVSYGYRCKGSGLWDKEDRFH 284
Query: 41 ASHVLLDPYAKLI 3
A HVLLDPYAK++
Sbjct: 285 AHHVLLDPYAKVL 297
>ref|XP_010278620.1| PREDICTED: isoamylase 2, chloroplastic [Nelumbo nucifera]
ref|XP_010278628.1| PREDICTED: isoamylase 2, chloroplastic [Nelumbo nucifera]
Length = 890
Score = 271 bits (694), Expect = 1e-81
Identities = 142/253 (56%), Positives = 170/253 (67%)
Frame = -1
Query: 761 LGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWG 582
LG E++++ +YLFRTE+GG VK+ VG N Y V IEVSSLP E L L+WG
Sbjct: 84 LGTFTKTEEVEKPLTYLFRTEIGGQVKITVGRKNTNYTVCIEVSSLPQCNTEDKLFLNWG 143
Query: 581 MFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXX 402
+FRSDSS L P SQ S +T ST + TP Q S G H + LEF+S Q P
Sbjct: 144 IFRSDSSCLTLPGSQVSAPETHSSTME-------TPLMQKSSGRHFLQLEFESNQAPFYL 196
Query: 401 XXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAES 222
+S I++HRKTNFCVPVGISSG P PLG S S+DG VNF+LFSR AES
Sbjct: 197 SFMLLFSSNTATNNSEIRSHRKTNFCVPVGISSGHPAPLGISFSDDGSVNFSLFSRNAES 256
Query: 221 VNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFH 42
V LCLY+ ++QPSL+I+LDPYINRTG+IWHVSMESV YVSYGYRCKG+I G RF
Sbjct: 257 VVLCLYDEKSDQPSLEIDLDPYINRTGNIWHVSMESVAPYVSYGYRCKGDIHLKKGDRFD 316
Query: 41 ASHVLLDPYAKLI 3
A +VLLDPYAK++
Sbjct: 317 ARNVLLDPYAKIL 329
>ref|XP_020700621.1| isoamylase 2, chloroplastic [Dendrobium catenatum]
gb|PKU77045.1| Isoamylase 2, chloroplastic [Dendrobium catenatum]
Length = 894
Score = 265 bits (678), Expect = 2e-79
Identities = 134/253 (52%), Positives = 170/253 (67%)
Frame = -1
Query: 761 LGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWG 582
L + + EK + +S LFRTE GLVKV VGA MKY V IEV SLP L H+LIL WG
Sbjct: 88 LEMTSDFEKQNKGYSLLFRTEFDGLVKVFVGAIKMKYTVCIEVQSLPQSLTSHNLILFWG 147
Query: 581 MFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXX 402
MFRSDS + PDS +S+L T++ + + ++TPF ++S H + L+FD VP
Sbjct: 148 MFRSDSLPFHGPDSHSSVLPTTKFKPEASQHLMKTPFVRDSC-RHILVLDFDQNDVPFYL 206
Query: 401 XXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAES 222
+ I+THRKTNFCVPVG+ G P+ LG S S DGL NF+LFSR A+S
Sbjct: 207 SFFLQSDGT--GQERTIRTHRKTNFCVPVGLGPGYPMALGVSSSNDGLANFSLFSRNAKS 264
Query: 221 VNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFH 42
V LC Y+S ++PSL+I+LDPY+N TGDIWHVS+ S+ NY+ YGYRCKG+I+WD GS +
Sbjct: 265 VVLCFYKSKTSEPSLEIDLDPYVNHTGDIWHVSVHSITNYIGYGYRCKGDISWDGGSSSN 324
Query: 41 ASHVLLDPYAKLI 3
A HVLLDPYAK I
Sbjct: 325 AHHVLLDPYAKSI 337
>gb|PIA39269.1| hypothetical protein AQUCO_02600010v1 [Aquilegia coerulea]
Length = 886
Score = 258 bits (660), Expect = 8e-77
Identities = 134/246 (54%), Positives = 165/246 (67%)
Frame = -1
Query: 740 EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSS 561
E L+ YLFRT+ GG VKV VG +MKY+V IEVS LP +++ +L+LSWG++RSDSS
Sbjct: 91 EDLENASMYLFRTDAGGQVKVYVGRKHMKYIVYIEVSFLPQCVSKKELLLSWGVYRSDSS 150
Query: 560 HLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXX 381
+ + LD STS + +TPF QNSLG + +EFDS Q P
Sbjct: 151 AM-------NPLDPQSSTSGAKSSISQTPFPQNSLGARTLQMEFDSIQAPFYFSFLLLCP 203
Query: 380 XXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLYE 201
+ IK+HRKTNFCVPVG+ SG P PLG S S DG VNFALFSR AE+V LCLY+
Sbjct: 204 SSAASDLKEIKSHRKTNFCVPVGLGSGSPAPLGISFSNDGSVNFALFSRNAENVVLCLYD 263
Query: 200 SGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLD 21
+ SL+I+LDPYINRTGDIWHVSMESV Y+SYGYRC+G I + G+ H SHVLLD
Sbjct: 264 EMEGKLSLEIDLDPYINRTGDIWHVSMESVAPYMSYGYRCRGTIPREKGNICHESHVLLD 323
Query: 20 PYAKLI 3
PYAK++
Sbjct: 324 PYAKVL 329
>ref|XP_010938750.1| PREDICTED: isoamylase 2, chloroplastic [Elaeis guineensis]
Length = 851
Score = 254 bits (648), Expect = 3e-75
Identities = 126/253 (49%), Positives = 163/253 (64%)
Frame = -1
Query: 761 LGLNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWG 582
L ++ + ++L++ FSY+FR E GGL KVLVG +MKY V +EVS LP + E L+++WG
Sbjct: 67 LEVSDSTQELEKPFSYIFRAETGGLAKVLVGVKDMKYAVRVEVSFLPRAVGEDGLVMNWG 126
Query: 581 MFRSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXX 402
MFRSDS L DSQ + F QNSLG + + LEFDS ++P
Sbjct: 127 MFRSDSKQLLVQDSQVA-------------------FVQNSLGGYMVELEFDSVEIPFYL 167
Query: 401 XXXXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAES 222
S I+TH+KT FCVPVGI G P+PLG S S+DG +NF++FSR AE
Sbjct: 168 SFLLSSPSV-----SEIRTHKKTKFCVPVGIGPGCPMPLGVSISDDGAINFSVFSRNAEG 222
Query: 221 VNLCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFH 42
V L L++ +PSL+I+LDPY+NRTGDIWHVSMES+ NY YGYRCKG WD G +FH
Sbjct: 223 VVLYLFDGRTREPSLEIDLDPYVNRTGDIWHVSMESIENYAGYGYRCKGLALWDKGDKFH 282
Query: 41 ASHVLLDPYAKLI 3
A +LLDPYAK++
Sbjct: 283 AHDILLDPYAKVL 295
>gb|PKA66852.1| Isoamylase 2, chloroplastic [Apostasia shenzhenica]
Length = 881
Score = 254 bits (648), Expect = 4e-75
Identities = 133/251 (52%), Positives = 158/251 (62%)
Frame = -1
Query: 755 LNYNAEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMF 576
+ +N E Q+ +SYLFRTE G+VKVLV N+MKY V IE+ SLP + +HDLIL W F
Sbjct: 89 VTFNFEDQQKEYSYLFRTEFDGMVKVLVRINDMKYTVYIELQSLPKPIIDHDLILCWHTF 148
Query: 575 RSDSSHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXX 396
RSDS + SQ S L ++TP T+ S G H LEF Q+P
Sbjct: 149 RSDSENSSGLGSQISALSADHF--------MKTPLTRGSSGMHTAVLEFSFQQIPFYLSF 200
Query: 395 XXXXXXXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVN 216
A S IKT RKT F VPVG+ SG +PLG S +DG NF+LFSR AESV
Sbjct: 201 LLQVDG---AAKSEIKTERKTYFRVPVGLGSGHSLPLGISLFDDGSANFSLFSRNAESVV 257
Query: 215 LCLYESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHAS 36
LCLY+ N+PS +IELDPY+NRTGDIWHV + + NY YGYRCKG+I WD GSRFHA
Sbjct: 258 LCLYDGKTNEPSFEIELDPYVNRTGDIWHVLLYDLSNYTRYGYRCKGKIFWDTGSRFHAR 317
Query: 35 HVLLDPYAKLI 3
HVLLDPYAK I
Sbjct: 318 HVLLDPYAKTI 328
>ref|XP_020087102.1| isoamylase 2, chloroplastic [Ananas comosus]
ref|XP_020087103.1| isoamylase 2, chloroplastic [Ananas comosus]
Length = 863
Score = 253 bits (647), Expect = 5e-75
Identities = 131/246 (53%), Positives = 164/246 (66%)
Frame = -1
Query: 740 EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSS 561
E+LQ+ FSY FRT+ G+VKV+V + KYVV EV+ LP + E L+L+W MF SDSS
Sbjct: 77 EELQKGFSYTFRTDSNGIVKVVVRSEYGKYVVRFEVTFLPQFGTEEGLVLNWTMFTSDSS 136
Query: 560 HLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXX 381
L P SQ S G + + PF QNS G+ L FD+++VP
Sbjct: 137 LLLVPCSQASE----------GTVTAKVPFVQNSSGSFLAELVFDASKVPFYLSFLLN-- 184
Query: 380 XXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLYE 201
+ S I++HRKTNFCVPVG+ SG+P+PLG S S+DGL NF+LFS+ AE V LCLY+
Sbjct: 185 ----SAGSEIRSHRKTNFCVPVGLGSGQPMPLGVSVSDDGLTNFSLFSKNAEGVILCLYD 240
Query: 200 SGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLD 21
++P+L+IELDPYIN TGDIWHVS+E+V YVSYGYRCKG I W G RFH HVLLD
Sbjct: 241 GIKDEPALEIELDPYINCTGDIWHVSLENVEEYVSYGYRCKGPILWRKGDRFHMKHVLLD 300
Query: 20 PYAKLI 3
PYAK+I
Sbjct: 301 PYAKVI 306
>gb|OAY83378.1| Isoamylase 2, chloroplastic [Ananas comosus]
Length = 1205
Score = 253 bits (647), Expect = 9e-74
Identities = 131/246 (53%), Positives = 164/246 (66%)
Frame = -1
Query: 740 EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSS 561
E+LQ+ FSY FRT+ G+VKV+V + KYVV EV+ LP + E L+L+W MF SDSS
Sbjct: 77 EELQKGFSYTFRTDSNGIVKVVVRSEYGKYVVRFEVTFLPQFGTEEGLVLNWTMFTSDSS 136
Query: 560 HLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXX 381
L P SQ S G + + PF QNS G+ L FD+++VP
Sbjct: 137 LLLVPCSQASE----------GTVTAKVPFVQNSSGSFLAELVFDASKVPFYLSFLLN-- 184
Query: 380 XXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLYE 201
+ S I++HRKTNFCVPVG+ SG+P+PLG S S+DGL NF+LFS+ AE V LCLY+
Sbjct: 185 ----SAGSEIRSHRKTNFCVPVGLGSGQPMPLGVSVSDDGLTNFSLFSKNAEGVILCLYD 240
Query: 200 SGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLD 21
++P+L+IELDPYIN TGDIWHVS+E+V YVSYGYRCKG I W G RFH HVLLD
Sbjct: 241 GIKDEPALEIELDPYINCTGDIWHVSLENVEEYVSYGYRCKGPILWRKGDRFHMKHVLLD 300
Query: 20 PYAKLI 3
PYAK+I
Sbjct: 301 PYAKVI 306
>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
Length = 882
Score = 246 bits (628), Expect = 3e-72
Identities = 123/247 (49%), Positives = 163/247 (65%)
Frame = -1
Query: 743 AEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDS 564
+E+L+ +YLF TE+GG VKV+VG N KY+V+IEVSSL LY +++ LILSWG+FRS+S
Sbjct: 91 SEELESRLNYLFLTEIGGQVKVIVGKKNKKYIVSIEVSSLQLYNSDNKLILSWGVFRSNS 150
Query: 563 SHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXX 384
S D Q + + +T+++ PF + S G+ A+ L+F++ P
Sbjct: 151 SCFMPVDFQNLVPEVGSNTTEI-------PFMERSSGSFALKLDFEANHAPFYLSFLLKS 203
Query: 383 XXXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLY 204
+ S I++HRKTNFC+PVG G P PLG S S DG NFA FSR A V LCLY
Sbjct: 204 TLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLSFSSDGSPNFAFFSRNAGGVVLCLY 263
Query: 203 ESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLL 24
+ +++P+L+I+LDPY+NRTGDIWH SMESVG++VSYGYRCK D+G H HV L
Sbjct: 264 DGTSDKPALEIDLDPYVNRTGDIWHASMESVGSFVSYGYRCKEANLQDSGETLHTEHVHL 323
Query: 23 DPYAKLI 3
DPYAKLI
Sbjct: 324 DPYAKLI 330
>ref|XP_020592870.1| isoamylase 2, chloroplastic [Phalaenopsis equestris]
Length = 861
Score = 243 bits (621), Expect = 2e-71
Identities = 129/246 (52%), Positives = 160/246 (65%)
Frame = -1
Query: 740 EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSS 561
EK + F +LFRTE GGLVKV VGA MKY V IEV SLP + H+LIL WGMFRSDSS
Sbjct: 85 EKQHKGFKFLFRTEFGGLVKVFVGAMEMKYTVCIEVHSLPQSMINHNLILLWGMFRSDSS 144
Query: 560 HLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXX 381
DS+ S+ +S++TPF S L+FD + VP
Sbjct: 145 VFQGSDSRISV------------SSMKTPFVGESC-----RLDFDHSNVPFYLSFLLLQP 187
Query: 380 XXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLYE 201
+S I+THRKTNFCVPVG+ G P+PLG S S DG NF+LFSR A++V LCLYE
Sbjct: 188 DDT-GFESAIRTHRKTNFCVPVGLGPGHPMPLGVSLSNDGWTNFSLFSRNAKTVVLCLYE 246
Query: 200 SGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLD 21
S N+P+L++ELDPY+NRTGD+WHVS++++ NY+ YGYRCKG+I HA HVLLD
Sbjct: 247 SETNEPTLELELDPYVNRTGDVWHVSIDNITNYILYGYRCKGDI--------HAHHVLLD 298
Query: 20 PYAKLI 3
PYAK+I
Sbjct: 299 PYAKII 304
>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
Length = 866
Score = 239 bits (609), Expect = 1e-69
Identities = 126/247 (51%), Positives = 164/247 (66%), Gaps = 1/247 (0%)
Frame = -1
Query: 740 EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSS 561
+K ++ +YLFRTE+GG VKVLV N+KY V +EVSSL L E L+++WG+FRSDSS
Sbjct: 80 QKEEKSSAYLFRTEIGGHVKVLVRQKNLKYSVYVEVSSLKLKSGEDKLVMNWGIFRSDSS 139
Query: 560 HLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXX 381
D Q+S D ST + TPF QNSLG I L F+ + P
Sbjct: 140 QFIPLDLQSSTPDDRSST-------VETPFVQNSLGKLVIELNFEGSLTPFYLSFLLKFP 192
Query: 380 XXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLY- 204
+ S +++HRKT+FCVPVG++SG P PLG S S DG +NFALFS+ A+ V LCLY
Sbjct: 193 SDGDSKSSDLRSHRKTSFCVPVGLASGYPAPLGLSFSADGSINFALFSQSADGVVLCLYD 252
Query: 203 ESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLL 24
++ A +P+L+I+LDPY+NR+GDIWHVSM+S +VSYGYRCKG I G + H+ HVLL
Sbjct: 253 DTKAEKPTLEIDLDPYVNRSGDIWHVSMDSALPFVSYGYRCKGGI-HGKGVKDHSEHVLL 311
Query: 23 DPYAKLI 3
DPYAK+I
Sbjct: 312 DPYAKVI 318
>ref|XP_020524299.1| isoamylase 2, chloroplastic isoform X5 [Amborella trichopoda]
Length = 831
Score = 236 bits (601), Expect = 1e-68
Identities = 117/239 (48%), Positives = 158/239 (66%)
Frame = -1
Query: 719 SYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDS 540
+++FRTE+GG VKV+V +M YV+++E+ SL ++ L L WG+FRSDSS DS
Sbjct: 98 TFMFRTEIGGQVKVVVSQKSMNYVISVELISLTDVCYQN-LELHWGIFRSDSSCWVLLDS 156
Query: 539 QTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATD 360
+ S T S+ G ++T F + G H++ L FDS Q P +
Sbjct: 157 ENSPSGTDLVKSESGEDCMKTLFKKTCSGEHSLELMFDSCQAPFYISFIVYSPFDSAWGN 216
Query: 359 SGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLYESGANQPS 180
S I+THRKTNF VPVGI G P+PLG S ++DG NF+LFSR AE+V LCLY+ +P+
Sbjct: 217 SQIRTHRKTNFVVPVGIGRGNPLPLGVSLNDDGSTNFSLFSRNAENVVLCLYDENTAKPA 276
Query: 179 LDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLI 3
L+IELDPYINR+GD+WHVS+ SV Y+SYG+RCKG I WD G+R+H +LLDPYAK++
Sbjct: 277 LEIELDPYINRSGDMWHVSLSSVRQYLSYGFRCKGAILWDKGNRYHMRRILLDPYAKIL 335
>ref|XP_020524298.1| isoamylase 2, chloroplastic isoform X4 [Amborella trichopoda]
Length = 874
Score = 236 bits (601), Expect = 2e-68
Identities = 117/239 (48%), Positives = 158/239 (66%)
Frame = -1
Query: 719 SYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDS 540
+++FRTE+GG VKV+V +M YV+++E+ SL ++ L L WG+FRSDSS DS
Sbjct: 98 TFMFRTEIGGQVKVVVSQKSMNYVISVELISLTDVCYQN-LELHWGIFRSDSSCWVLLDS 156
Query: 539 QTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATD 360
+ S T S+ G ++T F + G H++ L FDS Q P +
Sbjct: 157 ENSPSGTDLVKSESGEDCMKTLFKKTCSGEHSLELMFDSCQAPFYISFIVYSPFDSAWGN 216
Query: 359 SGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLYESGANQPS 180
S I+THRKTNF VPVGI G P+PLG S ++DG NF+LFSR AE+V LCLY+ +P+
Sbjct: 217 SQIRTHRKTNFVVPVGIGRGNPLPLGVSLNDDGSTNFSLFSRNAENVVLCLYDENTAKPA 276
Query: 179 LDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLI 3
L+IELDPYINR+GD+WHVS+ SV Y+SYG+RCKG I WD G+R+H +LLDPYAK++
Sbjct: 277 LEIELDPYINRSGDMWHVSLSSVRQYLSYGFRCKGAILWDKGNRYHMRRILLDPYAKIL 335
>ref|XP_011624242.1| isoamylase 2, chloroplastic isoform X2 [Amborella trichopoda]
Length = 891
Score = 236 bits (601), Expect = 2e-68
Identities = 117/239 (48%), Positives = 158/239 (66%)
Frame = -1
Query: 719 SYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDS 540
+++FRTE+GG VKV+V +M YV+++E+ SL ++ L L WG+FRSDSS DS
Sbjct: 98 TFMFRTEIGGQVKVVVSQKSMNYVISVELISLTDVCYQN-LELHWGIFRSDSSCWVLLDS 156
Query: 539 QTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATD 360
+ S T S+ G ++T F + G H++ L FDS Q P +
Sbjct: 157 ENSPSGTDLVKSESGEDCMKTLFKKTCSGEHSLELMFDSCQAPFYISFIVYSPFDSAWGN 216
Query: 359 SGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLYESGANQPS 180
S I+THRKTNF VPVGI G P+PLG S ++DG NF+LFSR AE+V LCLY+ +P+
Sbjct: 217 SQIRTHRKTNFVVPVGIGRGNPLPLGVSLNDDGSTNFSLFSRNAENVVLCLYDENTAKPA 276
Query: 179 LDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLI 3
L+IELDPYINR+GD+WHVS+ SV Y+SYG+RCKG I WD G+R+H +LLDPYAK++
Sbjct: 277 LEIELDPYINRSGDMWHVSLSSVRQYLSYGFRCKGAILWDKGNRYHMRRILLDPYAKIL 335
>gb|ERN08315.1| hypothetical protein AMTR_s00156p00067780 [Amborella trichopoda]
Length = 926
Score = 236 bits (601), Expect = 4e-68
Identities = 117/239 (48%), Positives = 158/239 (66%)
Frame = -1
Query: 719 SYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDS 540
+++FRTE+GG VKV+V +M YV+++E+ SL ++ L L WG+FRSDSS DS
Sbjct: 133 TFMFRTEIGGQVKVVVSQKSMNYVISVELISLTDVCYQN-LELHWGIFRSDSSCWVLLDS 191
Query: 539 QTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATD 360
+ S T S+ G ++T F + G H++ L FDS Q P +
Sbjct: 192 ENSPSGTDLVKSESGEDCMKTLFKKTCSGEHSLELMFDSCQAPFYISFIVYSPFDSAWGN 251
Query: 359 SGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLYESGANQPS 180
S I+THRKTNF VPVGI G P+PLG S ++DG NF+LFSR AE+V LCLY+ +P+
Sbjct: 252 SQIRTHRKTNFVVPVGIGRGNPLPLGVSLNDDGSTNFSLFSRNAENVVLCLYDENTAKPA 311
Query: 179 LDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLI 3
L+IELDPYINR+GD+WHVS+ SV Y+SYG+RCKG I WD G+R+H +LLDPYAK++
Sbjct: 312 LEIELDPYINRSGDMWHVSLSSVRQYLSYGFRCKGAILWDKGNRYHMRRILLDPYAKIL 370
>ref|XP_020524296.1| isoamylase 2, chloroplastic isoform X1 [Amborella trichopoda]
Length = 941
Score = 236 bits (601), Expect = 4e-68
Identities = 117/239 (48%), Positives = 158/239 (66%)
Frame = -1
Query: 719 SYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSSHLYAPDS 540
+++FRTE+GG VKV+V +M YV+++E+ SL ++ L L WG+FRSDSS DS
Sbjct: 98 TFMFRTEIGGQVKVVVSQKSMNYVISVELISLTDVCYQN-LELHWGIFRSDSSCWVLLDS 156
Query: 539 QTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXXXXXLATD 360
+ S T S+ G ++T F + G H++ L FDS Q P +
Sbjct: 157 ENSPSGTDLVKSESGEDCMKTLFKKTCSGEHSLELMFDSCQAPFYISFIVYSPFDSAWGN 216
Query: 359 SGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLYESGANQPS 180
S I+THRKTNF VPVGI G P+PLG S ++DG NF+LFSR AE+V LCLY+ +P+
Sbjct: 217 SQIRTHRKTNFVVPVGIGRGNPLPLGVSLNDDGSTNFSLFSRNAENVVLCLYDENTAKPA 276
Query: 179 LDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLLDPYAKLI 3
L+IELDPYINR+GD+WHVS+ SV Y+SYG+RCKG I WD G+R+H +LLDPYAK++
Sbjct: 277 LEIELDPYINRSGDMWHVSLSSVRQYLSYGFRCKGAILWDKGNRYHMRRILLDPYAKIL 335
>ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber]
ref|XP_023886632.1| isoamylase 2, chloroplastic [Quercus suber]
gb|POE68275.1| isoamylase 2, chloroplastic [Quercus suber]
Length = 887
Score = 232 bits (592), Expect = 5e-67
Identities = 119/248 (47%), Positives = 157/248 (63%), Gaps = 1/248 (0%)
Frame = -1
Query: 743 AEKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDS 564
A+ ++ +YLF TE+GG VKV V ++KY VNIEVSSL L +E LI SWG +R+DS
Sbjct: 92 ADNREKALTYLFWTEVGGQVKVFVRKTSVKYAVNIEVSSLQLQSSEDRLISSWGRYRADS 151
Query: 563 SHLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXX 384
S D Q+S+ D+ TS+ TPF Q S G A+ LEF++ Q+P
Sbjct: 152 SSFIPLDVQSSVPDSRTPTSE-------TPFIQTSAGRFALELEFEAKQIPFYLSFILKL 204
Query: 383 XXXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLY 204
+ S I++HRKT FCVPVG G P PLG S S DG +NFA+FSR E V LC Y
Sbjct: 205 PSDSGSRGSEIRSHRKTKFCVPVGFRKGYPAPLGLSFSPDGSMNFAIFSRNVEGVILCFY 264
Query: 203 ESG-ANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVL 27
+ A +P+L+++LDPYINR+GDIWH S ES +VSYGY+CKG + N ++F A ++
Sbjct: 265 DDAKAEEPALELDLDPYINRSGDIWHASFESAWTFVSYGYQCKGSLLQRNNNKFDAGQII 324
Query: 26 LDPYAKLI 3
LDPYAK+I
Sbjct: 325 LDPYAKII 332
>ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium]
Length = 883
Score = 228 bits (581), Expect = 2e-65
Identities = 119/247 (48%), Positives = 158/247 (63%), Gaps = 1/247 (0%)
Frame = -1
Query: 740 EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSS 561
E++ +V +YLFRTE+G +V V V N KY VNIEV SL L ++ L+L WG++R+DSS
Sbjct: 93 EEIDKVSTYLFRTEIGDVVNVFVRKRNAKYTVNIEVPSLYLSSSDRRLVLRWGIYRADSS 152
Query: 560 HLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXX 381
+ P LD ST + ++ TPFTQ S G LEF++ Q+P
Sbjct: 153 -CFVP------LDFKSSTPNDTTTTLETPFTQTSSGRFTFELEFEAKQIPFYFSFILTSP 205
Query: 380 XXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLY- 204
+D I++HRKTNFCVPVG G P PLG + S DG +NFA+FSR AESV LCLY
Sbjct: 206 ADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLTFSNDGSMNFAIFSRNAESVVLCLYD 265
Query: 203 ESGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLL 24
++ A +P+L+++LDPY+NR+GDIWHVS S +VSYGY+ KG + N + F HVLL
Sbjct: 266 DTTAEKPALELDLDPYVNRSGDIWHVSFGSARAFVSYGYKFKGNLLLTNKNNFDEGHVLL 325
Query: 23 DPYAKLI 3
DPYAK+I
Sbjct: 326 DPYAKVI 332
>ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]
gb|ONH93660.1| hypothetical protein PRUPE_8G244800 [Prunus persica]
Length = 883
Score = 227 bits (579), Expect = 3e-65
Identities = 118/247 (47%), Positives = 157/247 (63%), Gaps = 1/247 (0%)
Frame = -1
Query: 740 EKLQRVFSYLFRTELGGLVKVLVGANNMKYVVNIEVSSLPLYLNEHDLILSWGMFRSDSS 561
E++ +V +YLFRTE+G +V V V N KY VNIEV S L N+ L+L WGM+R+DSS
Sbjct: 93 EEIDKVSTYLFRTEIGDVVNVFVRKRNAKYTVNIEVPSSHLSSNDRRLVLRWGMYRADSS 152
Query: 560 HLYAPDSQTSMLDTSQSTSDVGRASIRTPFTQNSLGTHAITLEFDSAQVPXXXXXXXXXX 381
+ P LD ST + ++ TP Q S G + LEF++ Q+P
Sbjct: 153 -CFVP------LDFKSSTPNDTTTTLETPLIQTSSGRFTLELEFEAKQIPFYFSFILTSP 205
Query: 380 XXXLATDSGIKTHRKTNFCVPVGISSGRPIPLGFSKSEDGLVNFALFSRKAESVNLCLYE 201
+D I++HRKTNFCVPVG G P PLG + S DG +NFA+FSR AESV LCLY+
Sbjct: 206 ADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLTFSNDGSMNFAIFSRNAESVALCLYD 265
Query: 200 -SGANQPSLDIELDPYINRTGDIWHVSMESVGNYVSYGYRCKGEITWDNGSRFHASHVLL 24
+ A +P+L+++LDPY+NR+GDIWHVS S ++VSYGY+ KG + N + F HVLL
Sbjct: 266 NTTAEKPALELDLDPYVNRSGDIWHVSFGSAWSFVSYGYKFKGNLLLTNKNNFDEGHVLL 325
Query: 23 DPYAKLI 3
DPYAK+I
Sbjct: 326 DPYAKVI 332