BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon24_contig00028367
         (869 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020247136.1| LOW QUALITY PROTEIN: pullulanase 1, chloropl...   247   e-126
gb|ONK57269.1| uncharacterized protein A4U43_C10F18340 [Asparagu...   247   e-126
ref|XP_020084106.1| pullulanase 1, chloroplastic [Ananas comosus]     218   e-110
ref|XP_010918919.1| PREDICTED: pullulanase 1, chloroplastic isof...   219   e-109
ref|XP_010918920.1| PREDICTED: pullulanase 1, chloroplastic isof...   219   e-109
ref|XP_008807708.2| PREDICTED: pullulanase 1, chloroplastic, par...   213   e-108
ref|XP_010267258.1| PREDICTED: pullulanase 1, chloroplastic isof...   220   e-106
ref|XP_010267259.1| PREDICTED: pullulanase 1, chloroplastic isof...   220   e-106
ref|XP_010267260.1| PREDICTED: pullulanase 1, chloroplastic isof...   220   e-106
ref|XP_010267261.1| PREDICTED: pullulanase 1, chloroplastic isof...   220   e-106
gb|OVA17964.1| Glycoside hydrolase [Macleaya cordata]                 218   e-105
gb|OMO65703.1| hypothetical protein CCACVL1_21428, partial [Corc...   218   e-104
ref|XP_023879674.1| pullulanase 1, chloroplastic isoform X2 [Que...   207   e-103
ref|XP_021295295.1| pullulanase 1, chloroplastic isoform X2 [Her...   219   e-103
ref|XP_023879673.1| pullulanase 1, chloroplastic isoform X1 [Que...   207   e-103
ref|XP_023879675.1| pullulanase 1, chloroplastic isoform X3 [Que...   207   e-103
ref|XP_021295293.1| pullulanase 1, chloroplastic isoform X1 [Her...   219   e-102
ref|XP_021295296.1| pullulanase 1, chloroplastic isoform X3 [Her...   219   e-102
ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isof...   218   e-102
ref|XP_016685975.1| PREDICTED: pullulanase 1, chloroplastic-like...   211   e-102

>ref|XP_020247136.1| LOW QUALITY PROTEIN: pullulanase 1, chloroplastic [Asparagus
           officinalis]
          Length = 960

 Score =  247 bits (630), Expect(2) = e-126
 Identities = 124/156 (79%), Positives = 129/156 (82%), Gaps = 1/156 (0%)
 Frame = +3

Query: 3   AVEESLSQDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQ 182
           A E  LSQDCLS ARAYWVTRS+IAWN  DDE  VWLYASR+A L +T EGVEG + K  
Sbjct: 70  AAERFLSQDCLSTARAYWVTRSLIAWNVGDDEAVVWLYASRRANLVLTDEGVEGHDSKVL 129

Query: 183 LEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSF-GVGKCMNATGLQL 359
           LE  PCGLPENV+QKFPHIK YKAFHVP SVDVG LVKCQLAVVSF   GKC NATGLQL
Sbjct: 130 LEQEPCGLPENVIQKFPHIKHYKAFHVPLSVDVGSLVKCQLAVVSFCARGKCKNATGLQL 189

Query: 360 PGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGC 467
           PGVLDDMFAY GPLGAVFSEEAITL LWAPTAQV C
Sbjct: 190 PGVLDDMFAYKGPLGAVFSEEAITLNLWAPTAQVVC 225



 Score =  234 bits (598), Expect(2) = e-126
 Identities = 108/135 (80%), Positives = 121/135 (89%)
 Frame = +1

Query: 463 VVHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKV 642
           VV AHVYK P G D+LD +QL+E++GVWSA GPRSWEGCYYVYEVSVYHPSTS+IETCKV
Sbjct: 223 VVCAHVYKGPLGEDQLDPMQLEEVNGVWSAAGPRSWEGCYYVYEVSVYHPSTSRIETCKV 282

Query: 643 NDPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASD 822
           NDPYARG+SSDGMRTLLVNLDC + KP GWD+L   KP+  SF+DISIYELHVRDFSASD
Sbjct: 283 NDPYARGLSSDGMRTLLVNLDCGKFKPPGWDDLANGKPEFHSFADISIYELHVRDFSASD 342

Query: 823 ETVNPDFRGGFLAFT 867
           ETV+P+FRGGFLAFT
Sbjct: 343 ETVDPNFRGGFLAFT 357


>gb|ONK57269.1| uncharacterized protein A4U43_C10F18340 [Asparagus officinalis]
          Length = 947

 Score =  247 bits (630), Expect(2) = e-126
 Identities = 124/156 (79%), Positives = 129/156 (82%), Gaps = 1/156 (0%)
 Frame = +3

Query: 3   AVEESLSQDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQ 182
           A E  LSQDCLS ARAYWVTRS+IAWN  DDE  VWLYASR+A L +T EGVEG + K  
Sbjct: 70  AAERFLSQDCLSTARAYWVTRSLIAWNVGDDEAVVWLYASRRANLVLTDEGVEGHDSKVL 129

Query: 183 LEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSF-GVGKCMNATGLQL 359
           LE  PCGLPENV+QKFPHIK YKAFHVP SVDVG LVKCQLAVVSF   GKC NATGLQL
Sbjct: 130 LEQEPCGLPENVIQKFPHIKHYKAFHVPLSVDVGSLVKCQLAVVSFCARGKCKNATGLQL 189

Query: 360 PGVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGC 467
           PGVLDDMFAY GPLGAVFSEEAITL LWAPTAQV C
Sbjct: 190 PGVLDDMFAYKGPLGAVFSEEAITLNLWAPTAQVVC 225



 Score =  234 bits (598), Expect(2) = e-126
 Identities = 108/135 (80%), Positives = 121/135 (89%)
 Frame = +1

Query: 463 VVHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKV 642
           VV AHVYK P G D+LD +QL+E++GVWSA GPRSWEGCYYVYEVSVYHPSTS+IETCKV
Sbjct: 223 VVCAHVYKGPLGEDQLDPMQLEEVNGVWSAAGPRSWEGCYYVYEVSVYHPSTSRIETCKV 282

Query: 643 NDPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASD 822
           NDPYARG+SSDGMRTLLVNLDC + KP GWD+L   KP+  SF+DISIYELHVRDFSASD
Sbjct: 283 NDPYARGLSSDGMRTLLVNLDCGKFKPPGWDDLANGKPEFHSFADISIYELHVRDFSASD 342

Query: 823 ETVNPDFRGGFLAFT 867
           ETV+P+FRGGFLAFT
Sbjct: 343 ETVDPNFRGGFLAFT 357


>ref|XP_020084106.1| pullulanase 1, chloroplastic [Ananas comosus]
          Length = 986

 Score =  218 bits (554), Expect(2) = e-110
 Identities = 107/156 (68%), Positives = 125/156 (80%), Gaps = 1/156 (0%)
 Frame = +3

Query: 9   EESLSQDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLE 188
           E  LSQD LSY+RAYWV RS+IAWN D+D+ S++LYAS+KA +F++ +GV G +VK QLE
Sbjct: 98  ESPLSQDLLSYSRAYWVARSLIAWNVDEDDNSIYLYASKKANMFLSDDGVGGYDVKIQLE 157

Query: 189 PGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSF-GVGKCMNATGLQLPG 365
           P  C LP NV +KFPHIKDY+AF VP+S DV +L+K QLAV SF   GKC NATGLQLPG
Sbjct: 158 PDHCALPANVTEKFPHIKDYRAFKVPNS-DVQNLLKSQLAVASFYANGKCKNATGLQLPG 216

Query: 366 VLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 473
           VLDDMFAY GPLGAVFS+EAI LYLWAPTAQ    C
Sbjct: 217 VLDDMFAYNGPLGAVFSKEAIALYLWAPTAQDVSAC 252



 Score =  211 bits (538), Expect(2) = e-110
 Identities = 98/134 (73%), Positives = 113/134 (84%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           V A  Y+DPSGG  L+ +QL+EL+GVWS VGPRSW G YYVYE+SVYHPSTS+IE C+ N
Sbjct: 249 VSACFYEDPSGGVPLECIQLKELNGVWSVVGPRSWAGRYYVYELSVYHPSTSRIEKCESN 308

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RT LV+LDCE LKP GWDEL ++KP L SFSDISIYELH+RDFSASD 
Sbjct: 309 DPYARGLSSDGKRTWLVDLDCEDLKPAGWDELADQKPSLHSFSDISIYELHIRDFSASDH 368

Query: 826 TVNPDFRGGFLAFT 867
           TV+PD RGG+ AFT
Sbjct: 369 TVDPDSRGGYRAFT 382


>ref|XP_010918919.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Elaeis
           guineensis]
          Length = 965

 Score =  219 bits (559), Expect(2) = e-109
 Identities = 100/130 (76%), Positives = 114/130 (87%)
 Frame = +1

Query: 478 VYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVNDPYA 657
           +YK+PSGG+ L+SVQL+E  GVWSAVGP+SWEGCYYVYEVSVYHPSTSQIE C V DPYA
Sbjct: 233 IYKEPSGGEPLESVQLEEFGGVWSAVGPKSWEGCYYVYEVSVYHPSTSQIEKCTVTDPYA 292

Query: 658 RGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDETVNP 837
           RG+SS+G RT L+NLD E LKP GWD+L +EKPDL SFSDISIYELH+RDFSASD TVN 
Sbjct: 293 RGLSSNGKRTWLINLDSENLKPEGWDKLADEKPDLLSFSDISIYELHIRDFSASDHTVNC 352

Query: 838 DFRGGFLAFT 867
           D +GG+LAFT
Sbjct: 353 DIQGGYLAFT 362



 Score =  205 bits (521), Expect(2) = e-109
 Identities = 100/156 (64%), Positives = 121/156 (77%), Gaps = 1/156 (0%)
 Frame = +3

Query: 9   EESLSQDCLSYARAYWVTRSIIAWNAD-DDETSVWLYASRKAQLFVTSEGVEGQEVKFQL 185
           E SLSQ+ LSYARAYWV+R +IAWN + DD+  V+LYASRKA + +T EG+EG +VK  L
Sbjct: 77  ESSLSQETLSYARAYWVSRILIAWNINYDDDAYVYLYASRKASISLTEEGIEGYDVKILL 136

Query: 186 EPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVGKCMNATGLQLPG 365
           EP   GLP NV +KFPHI +Y AF +  SVD  +L+KCQLA+  +  GKC NATGLQLPG
Sbjct: 137 EPEYYGLPANVTEKFPHIVNYSAFKISESVDFENLLKCQLAIAFYVDGKCKNATGLQLPG 196

Query: 366 VLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 473
           VLDD+FAY GPLGAVFS+EA+TL+LWAPTAQ    C
Sbjct: 197 VLDDLFAYNGPLGAVFSKEAVTLHLWAPTAQEVSVC 232


>ref|XP_010918920.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Elaeis
           guineensis]
          Length = 946

 Score =  219 bits (559), Expect(2) = e-109
 Identities = 100/130 (76%), Positives = 114/130 (87%)
 Frame = +1

Query: 478 VYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVNDPYA 657
           +YK+PSGG+ L+SVQL+E  GVWSAVGP+SWEGCYYVYEVSVYHPSTSQIE C V DPYA
Sbjct: 233 IYKEPSGGEPLESVQLEEFGGVWSAVGPKSWEGCYYVYEVSVYHPSTSQIEKCTVTDPYA 292

Query: 658 RGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDETVNP 837
           RG+SS+G RT L+NLD E LKP GWD+L +EKPDL SFSDISIYELH+RDFSASD TVN 
Sbjct: 293 RGLSSNGKRTWLINLDSENLKPEGWDKLADEKPDLLSFSDISIYELHIRDFSASDHTVNC 352

Query: 838 DFRGGFLAFT 867
           D +GG+LAFT
Sbjct: 353 DIQGGYLAFT 362



 Score =  205 bits (521), Expect(2) = e-109
 Identities = 100/156 (64%), Positives = 121/156 (77%), Gaps = 1/156 (0%)
 Frame = +3

Query: 9   EESLSQDCLSYARAYWVTRSIIAWNAD-DDETSVWLYASRKAQLFVTSEGVEGQEVKFQL 185
           E SLSQ+ LSYARAYWV+R +IAWN + DD+  V+LYASRKA + +T EG+EG +VK  L
Sbjct: 77  ESSLSQETLSYARAYWVSRILIAWNINYDDDAYVYLYASRKASISLTEEGIEGYDVKILL 136

Query: 186 EPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVGKCMNATGLQLPG 365
           EP   GLP NV +KFPHI +Y AF +  SVD  +L+KCQLA+  +  GKC NATGLQLPG
Sbjct: 137 EPEYYGLPANVTEKFPHIVNYSAFKISESVDFENLLKCQLAIAFYVDGKCKNATGLQLPG 196

Query: 366 VLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 473
           VLDD+FAY GPLGAVFS+EA+TL+LWAPTAQ    C
Sbjct: 197 VLDDLFAYNGPLGAVFSKEAVTLHLWAPTAQEVSVC 232


>ref|XP_008807708.2| PREDICTED: pullulanase 1, chloroplastic, partial [Phoenix
           dactylifera]
          Length = 548

 Score =  213 bits (542), Expect(2) = e-108
 Identities = 100/130 (76%), Positives = 112/130 (86%)
 Frame = +1

Query: 478 VYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVNDPYA 657
           VY+DP GG+ ++SVQL+E  GVWSAVGPRSWEGCYYVYEVSVYHPSTS+IE   VNDPYA
Sbjct: 165 VYEDPLGGEPVESVQLEEFGGVWSAVGPRSWEGCYYVYEVSVYHPSTSRIEKSTVNDPYA 224

Query: 658 RGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDETVNP 837
           RG+SS+G RT LVNLD E LKP GWD+L +EKP L SFSDISIYELH+RDFSASD TVN 
Sbjct: 225 RGLSSNGKRTWLVNLDSENLKPEGWDKLADEKPGLLSFSDISIYELHIRDFSASDYTVNC 284

Query: 838 DFRGGFLAFT 867
           D RGG+LAFT
Sbjct: 285 DIRGGYLAFT 294



 Score =  209 bits (531), Expect(2) = e-108
 Identities = 104/157 (66%), Positives = 122/157 (77%), Gaps = 2/157 (1%)
 Frame = +3

Query: 9   EESLSQDCLSYARAYWVTRSIIAWNA-DDDETSVWLYASRKAQLFVTSEGVEG-QEVKFQ 182
           E SL QD LSYARAYWV R +IAWN  DDD+  V+LYASRKA +F+T EG+EG  +VK  
Sbjct: 8   EVSLPQDLLSYARAYWVARFLIAWNINDDDDACVYLYASRKASIFLTDEGIEGGYDVKIL 67

Query: 183 LEPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGVGKCMNATGLQLP 362
           LEP   GLP NV +KFPHI +Y+AF +  SVD+G+L+KCQLAV  +  GKC NATGLQLP
Sbjct: 68  LEPEYNGLPANVTEKFPHIVNYRAFKISQSVDIGNLLKCQLAVAFYVDGKCENATGLQLP 127

Query: 363 GVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 473
           GVLDD+FAY GPLGAVFS+ A+TLYLWAPTAQ    C
Sbjct: 128 GVLDDLFAYNGPLGAVFSKAAVTLYLWAPTAQEVSVC 164


>ref|XP_010267258.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nelumbo
           nucifera]
          Length = 958

 Score =  220 bits (561), Expect(2) = e-106
 Identities = 102/134 (76%), Positives = 115/134 (85%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           V A +Y D SGG  L+SVQL+E+ GVWSA GPR+WEGCYYVYEVSVYHPST +IETC  N
Sbjct: 223 VSAIIYGDSSGGVPLESVQLEEVDGVWSAHGPRNWEGCYYVYEVSVYHPSTLRIETCMAN 282

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RTLLVNLDC+ LKP GWD L +EKP L SFSDISIYELH+RDFSASD+
Sbjct: 283 DPYARGLSSDGKRTLLVNLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQ 342

Query: 826 TVNPDFRGGFLAFT 867
           TV+PDF GG+LAFT
Sbjct: 343 TVHPDFHGGYLAFT 356



 Score =  196 bits (497), Expect(2) = e-106
 Identities = 97/146 (66%), Positives = 115/146 (78%), Gaps = 1/146 (0%)
 Frame = +3

Query: 24  QDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPGPCG 203
           Q  L Y+RAYWVTRS+IAWN D  + S +LYASR A L VT +G++G EVK +LE    G
Sbjct: 76  QCSLLYSRAYWVTRSLIAWNIDIADGSCYLYASRTAGLVVTEDGIQGHEVKIKLEVENSG 135

Query: 204 LPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSF-GVGKCMNATGLQLPGVLDDM 380
           LP NVV+KFPHI+  + F VP SVDV  LVKCQLAV SF   G+C NATGLQLPGV+DD+
Sbjct: 136 LPSNVVEKFPHIRHNRVFRVPTSVDVKSLVKCQLAVASFHSDGQCSNATGLQLPGVIDDL 195

Query: 381 FAYGGPLGAVFSEEAITLYLWAPTAQ 458
           FAYGGPLGAVF++EA++LYLWAPTAQ
Sbjct: 196 FAYGGPLGAVFTKEAVSLYLWAPTAQ 221


>ref|XP_010267259.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Nelumbo
           nucifera]
          Length = 941

 Score =  220 bits (561), Expect(2) = e-106
 Identities = 102/134 (76%), Positives = 115/134 (85%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           V A +Y D SGG  L+SVQL+E+ GVWSA GPR+WEGCYYVYEVSVYHPST +IETC  N
Sbjct: 223 VSAIIYGDSSGGVPLESVQLEEVDGVWSAHGPRNWEGCYYVYEVSVYHPSTLRIETCMAN 282

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RTLLVNLDC+ LKP GWD L +EKP L SFSDISIYELH+RDFSASD+
Sbjct: 283 DPYARGLSSDGKRTLLVNLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQ 342

Query: 826 TVNPDFRGGFLAFT 867
           TV+PDF GG+LAFT
Sbjct: 343 TVHPDFHGGYLAFT 356



 Score =  196 bits (497), Expect(2) = e-106
 Identities = 97/146 (66%), Positives = 115/146 (78%), Gaps = 1/146 (0%)
 Frame = +3

Query: 24  QDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPGPCG 203
           Q  L Y+RAYWVTRS+IAWN D  + S +LYASR A L VT +G++G EVK +LE    G
Sbjct: 76  QCSLLYSRAYWVTRSLIAWNIDIADGSCYLYASRTAGLVVTEDGIQGHEVKIKLEVENSG 135

Query: 204 LPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSF-GVGKCMNATGLQLPGVLDDM 380
           LP NVV+KFPHI+  + F VP SVDV  LVKCQLAV SF   G+C NATGLQLPGV+DD+
Sbjct: 136 LPSNVVEKFPHIRHNRVFRVPTSVDVKSLVKCQLAVASFHSDGQCSNATGLQLPGVIDDL 195

Query: 381 FAYGGPLGAVFSEEAITLYLWAPTAQ 458
           FAYGGPLGAVF++EA++LYLWAPTAQ
Sbjct: 196 FAYGGPLGAVFTKEAVSLYLWAPTAQ 221


>ref|XP_010267260.1| PREDICTED: pullulanase 1, chloroplastic isoform X3 [Nelumbo
           nucifera]
          Length = 932

 Score =  220 bits (561), Expect(2) = e-106
 Identities = 102/134 (76%), Positives = 115/134 (85%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           V A +Y D SGG  L+SVQL+E+ GVWSA GPR+WEGCYYVYEVSVYHPST +IETC  N
Sbjct: 223 VSAIIYGDSSGGVPLESVQLEEVDGVWSAHGPRNWEGCYYVYEVSVYHPSTLRIETCMAN 282

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RTLLVNLDC+ LKP GWD L +EKP L SFSDISIYELH+RDFSASD+
Sbjct: 283 DPYARGLSSDGKRTLLVNLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQ 342

Query: 826 TVNPDFRGGFLAFT 867
           TV+PDF GG+LAFT
Sbjct: 343 TVHPDFHGGYLAFT 356



 Score =  196 bits (497), Expect(2) = e-106
 Identities = 97/146 (66%), Positives = 115/146 (78%), Gaps = 1/146 (0%)
 Frame = +3

Query: 24  QDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPGPCG 203
           Q  L Y+RAYWVTRS+IAWN D  + S +LYASR A L VT +G++G EVK +LE    G
Sbjct: 76  QCSLLYSRAYWVTRSLIAWNIDIADGSCYLYASRTAGLVVTEDGIQGHEVKIKLEVENSG 135

Query: 204 LPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSF-GVGKCMNATGLQLPGVLDDM 380
           LP NVV+KFPHI+  + F VP SVDV  LVKCQLAV SF   G+C NATGLQLPGV+DD+
Sbjct: 136 LPSNVVEKFPHIRHNRVFRVPTSVDVKSLVKCQLAVASFHSDGQCSNATGLQLPGVIDDL 195

Query: 381 FAYGGPLGAVFSEEAITLYLWAPTAQ 458
           FAYGGPLGAVF++EA++LYLWAPTAQ
Sbjct: 196 FAYGGPLGAVFTKEAVSLYLWAPTAQ 221


>ref|XP_010267261.1| PREDICTED: pullulanase 1, chloroplastic isoform X4 [Nelumbo
           nucifera]
          Length = 854

 Score =  220 bits (561), Expect(2) = e-106
 Identities = 102/134 (76%), Positives = 115/134 (85%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           V A +Y D SGG  L+SVQL+E+ GVWSA GPR+WEGCYYVYEVSVYHPST +IETC  N
Sbjct: 223 VSAIIYGDSSGGVPLESVQLEEVDGVWSAHGPRNWEGCYYVYEVSVYHPSTLRIETCMAN 282

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RTLLVNLDC+ LKP GWD L +EKP L SFSDISIYELH+RDFSASD+
Sbjct: 283 DPYARGLSSDGKRTLLVNLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQ 342

Query: 826 TVNPDFRGGFLAFT 867
           TV+PDF GG+LAFT
Sbjct: 343 TVHPDFHGGYLAFT 356



 Score =  196 bits (497), Expect(2) = e-106
 Identities = 97/146 (66%), Positives = 115/146 (78%), Gaps = 1/146 (0%)
 Frame = +3

Query: 24  QDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPGPCG 203
           Q  L Y+RAYWVTRS+IAWN D  + S +LYASR A L VT +G++G EVK +LE    G
Sbjct: 76  QCSLLYSRAYWVTRSLIAWNIDIADGSCYLYASRTAGLVVTEDGIQGHEVKIKLEVENSG 135

Query: 204 LPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSF-GVGKCMNATGLQLPGVLDDM 380
           LP NVV+KFPHI+  + F VP SVDV  LVKCQLAV SF   G+C NATGLQLPGV+DD+
Sbjct: 136 LPSNVVEKFPHIRHNRVFRVPTSVDVKSLVKCQLAVASFHSDGQCSNATGLQLPGVIDDL 195

Query: 381 FAYGGPLGAVFSEEAITLYLWAPTAQ 458
           FAYGGPLGAVF++EA++LYLWAPTAQ
Sbjct: 196 FAYGGPLGAVFTKEAVSLYLWAPTAQ 221


>gb|OVA17964.1| Glycoside hydrolase [Macleaya cordata]
          Length = 983

 Score =  218 bits (555), Expect(2) = e-105
 Identities = 95/135 (70%), Positives = 118/135 (87%)
 Frame = +1

Query: 463 VVHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKV 642
           VV A +Y++PSGG  ++ ++L+E++GVWSA G RSWEGCYYVYEVSVYHPSTS +ETC  
Sbjct: 246 VVRACIYREPSGGSPIEIIRLEEVNGVWSAHGSRSWEGCYYVYEVSVYHPSTSLVETCIA 305

Query: 643 NDPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASD 822
           NDPYARG+SSDG RTLLVNLDC+ LKP GW++L ++KP+  SFSD+SIYELH+RDFSA+D
Sbjct: 306 NDPYARGLSSDGKRTLLVNLDCDSLKPEGWEKLVDQKPEFSSFSDVSIYELHIRDFSAND 365

Query: 823 ETVNPDFRGGFLAFT 867
            TVNPDF+GG+LAFT
Sbjct: 366 HTVNPDFQGGYLAFT 380



 Score =  192 bits (489), Expect(2) = e-105
 Identities = 91/151 (60%), Positives = 118/151 (78%), Gaps = 1/151 (0%)
 Frame = +3

Query: 24  QDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPGPCG 203
           Q  LSY+RAYWV  S+IAWN D  + S +LYAS+ A L +T++G+EG +VK +LEP   G
Sbjct: 100 QGSLSYSRAYWVNGSLIAWNVDIGDGSCYLYASKTANLVLTNDGIEGYDVKIKLEPDHSG 159

Query: 204 LPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGV-GKCMNATGLQLPGVLDDM 380
           LP NV++KFPHI++Y  F VP  +D  +L+KCQLAVV+F   G+C +ATGLQLPGVLDD+
Sbjct: 160 LPVNVIEKFPHIQNYNVFKVPFGLDAKNLLKCQLAVVAFKADGQCSDATGLQLPGVLDDL 219

Query: 381 FAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 473
           F+Y GPLGAVFS+EA++LY+WAPTAQV   C
Sbjct: 220 FSYVGPLGAVFSKEAVSLYIWAPTAQVVRAC 250


>gb|OMO65703.1| hypothetical protein CCACVL1_21428, partial [Corchorus capsularis]
          Length = 702

 Score =  218 bits (554), Expect(2) = e-104
 Identities = 98/134 (73%), Positives = 112/134 (83%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           VHAH+YKDP GG  L+ + L+E +GVWS  GP+SWEGCYYVYEVSVYHPST QIE C  N
Sbjct: 258 VHAHIYKDPVGGGPLEIIPLEETNGVWSTKGPKSWEGCYYVYEVSVYHPSTLQIEKCYAN 317

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RTL VNLD   LKP GWDEL ++KPD+ SFSDISIYELH+RDFSA+D 
Sbjct: 318 DPYARGLSSDGRRTLFVNLDTNGLKPEGWDELADKKPDIDSFSDISIYELHIRDFSANDN 377

Query: 826 TVNPDFRGGFLAFT 867
           TV P+FRGG+LAFT
Sbjct: 378 TVYPEFRGGYLAFT 391



 Score =  190 bits (482), Expect(2) = e-104
 Identities = 88/146 (60%), Positives = 119/146 (81%), Gaps = 1/146 (0%)
 Frame = +3

Query: 24  QDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPGPCG 203
           QD L Y+RA+WV++SIIAWN D  + S +LYAS+ A L VT +G++G +VK +LE    G
Sbjct: 111 QDNLLYSRAFWVSKSIIAWNVDVVDGSCYLYASKVAALSVTDDGIQGHDVKIKLEEDCSG 170

Query: 204 LPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGV-GKCMNATGLQLPGVLDDM 380
           LP+NV++KFPHI+DY+AF VP ++DV +L+KCQLAV +F   GKC NATGLQLPG+LD++
Sbjct: 171 LPQNVIEKFPHIRDYRAFKVPPALDVKNLLKCQLAVAAFNSHGKCSNATGLQLPGILDEL 230

Query: 381 FAYGGPLGAVFSEEAITLYLWAPTAQ 458
           F+Y GPLGA++S E+++LYLWAPTAQ
Sbjct: 231 FSYDGPLGALYSGESVSLYLWAPTAQ 256


>ref|XP_023879674.1| pullulanase 1, chloroplastic isoform X2 [Quercus suber]
 gb|POE76574.1| pullulanase 1, chloroplastic [Quercus suber]
          Length = 961

 Score =  207 bits (526), Expect(2) = e-103
 Identities = 94/134 (70%), Positives = 112/134 (83%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           V A +Y+DP GG+ L+ VQL+E++GVWS  GP+ WEGCYYVYEVSVYHPST +I+ C VN
Sbjct: 227 VCACIYRDPVGGNALEIVQLEEVNGVWSTKGPKRWEGCYYVYEVSVYHPSTLRIDKCHVN 286

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RT LVNLD + LKP  WD L  EKPD+ SFSDISIYELH+RDFSA+D 
Sbjct: 287 DPYARGLSSDGRRTFLVNLDSDTLKPEEWDNLANEKPDILSFSDISIYELHIRDFSANDY 346

Query: 826 TVNPDFRGGFLAFT 867
           TV+P+FRGG+LAFT
Sbjct: 347 TVHPEFRGGYLAFT 360



 Score =  199 bits (506), Expect(2) = e-103
 Identities = 90/152 (59%), Positives = 121/152 (79%), Gaps = 1/152 (0%)
 Frame = +3

Query: 21  SQDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPGPC 200
           SQD L ++RAYWV++S IAWN D  + S +L+AS+ A L +  +GVEG++++ +LE   C
Sbjct: 79  SQDSLLFSRAYWVSKSTIAWNVDVGDGSCFLFASKTAALSIVDDGVEGKDMQIKLEEDSC 138

Query: 201 GLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGV-GKCMNATGLQLPGVLDD 377
           GLPENV++KFPHI+DY+AF VP ++D   L+KCQLAV +F   GKC+N TGLQLPGVLDD
Sbjct: 139 GLPENVIRKFPHIQDYRAFKVPSTLDAKTLLKCQLAVATFNSDGKCINVTGLQLPGVLDD 198

Query: 378 MFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 473
           +F+Y GPLGA++SEE+++LYLWAPTAQ  C C
Sbjct: 199 LFSYNGPLGALYSEESVSLYLWAPTAQAVCAC 230


>ref|XP_021295295.1| pullulanase 1, chloroplastic isoform X2 [Herrania umbratica]
          Length = 963

 Score =  219 bits (557), Expect(2) = e-103
 Identities = 98/134 (73%), Positives = 112/134 (83%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           VHAH+YKDP GG  L+ +QL E +GVWS  GP+ WEGCYYVYEVSVYHPST QIE C  N
Sbjct: 221 VHAHIYKDPVGGSPLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYAN 280

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RTL VNLD   LKP GWDEL ++KPD+ SFSDISIYELH+RDFSA+D+
Sbjct: 281 DPYARGLSSDGRRTLFVNLDSNSLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDK 340

Query: 826 TVNPDFRGGFLAFT 867
           TVNPDF GG++AFT
Sbjct: 341 TVNPDFCGGYMAFT 354



 Score =  187 bits (474), Expect(2) = e-103
 Identities = 87/151 (57%), Positives = 117/151 (77%), Gaps = 1/151 (0%)
 Frame = +3

Query: 9   EESLSQDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLE 188
           + S SQD L Y+RAYWV+++IIAWN D  + S +LYAS+ A L VT +G++G + + +LE
Sbjct: 69  DSSSSQDSLLYSRAYWVSKTIIAWNVDVGDGSCYLYASKVAALSVTDDGIQGLDAEIKLE 128

Query: 189 PGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGV-GKCMNATGLQLPG 365
               GLP NV++KFPHI++Y+AF VP ++D   L+KCQLAV +F   GKC NATGLQLPG
Sbjct: 129 EDSTGLPPNVIEKFPHIQNYRAFKVPPALDTKSLLKCQLAVAAFNSHGKCSNATGLQLPG 188

Query: 366 VLDDMFAYGGPLGAVFSEEAITLYLWAPTAQ 458
           +LD++F+Y GPLGA + EEA++LYLWAPTAQ
Sbjct: 189 ILDELFSYDGPLGAFYLEEAVSLYLWAPTAQ 219


>ref|XP_023879673.1| pullulanase 1, chloroplastic isoform X1 [Quercus suber]
 gb|POE76573.1| pullulanase 1, chloroplastic [Quercus suber]
          Length = 963

 Score =  207 bits (526), Expect(2) = e-103
 Identities = 94/134 (70%), Positives = 112/134 (83%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           V A +Y+DP GG+ L+ VQL+E++GVWS  GP+ WEGCYYVYEVSVYHPST +I+ C VN
Sbjct: 229 VCACIYRDPVGGNALEIVQLEEVNGVWSTKGPKRWEGCYYVYEVSVYHPSTLRIDKCHVN 288

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RT LVNLD + LKP  WD L  EKPD+ SFSDISIYELH+RDFSA+D 
Sbjct: 289 DPYARGLSSDGRRTFLVNLDSDTLKPEEWDNLANEKPDILSFSDISIYELHIRDFSANDY 348

Query: 826 TVNPDFRGGFLAFT 867
           TV+P+FRGG+LAFT
Sbjct: 349 TVHPEFRGGYLAFT 362



 Score =  197 bits (502), Expect(2) = e-103
 Identities = 89/151 (58%), Positives = 120/151 (79%), Gaps = 1/151 (0%)
 Frame = +3

Query: 24  QDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPGPCG 203
           QD L ++RAYWV++S IAWN D  + S +L+AS+ A L +  +GVEG++++ +LE   CG
Sbjct: 82  QDSLLFSRAYWVSKSTIAWNVDVGDGSCFLFASKTAALSIVDDGVEGKDMQIKLEEDSCG 141

Query: 204 LPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGV-GKCMNATGLQLPGVLDDM 380
           LPENV++KFPHI+DY+AF VP ++D   L+KCQLAV +F   GKC+N TGLQLPGVLDD+
Sbjct: 142 LPENVIRKFPHIQDYRAFKVPSTLDAKTLLKCQLAVATFNSDGKCINVTGLQLPGVLDDL 201

Query: 381 FAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 473
           F+Y GPLGA++SEE+++LYLWAPTAQ  C C
Sbjct: 202 FSYNGPLGALYSEESVSLYLWAPTAQAVCAC 232


>ref|XP_023879675.1| pullulanase 1, chloroplastic isoform X3 [Quercus suber]
          Length = 825

 Score =  207 bits (526), Expect(2) = e-103
 Identities = 94/134 (70%), Positives = 112/134 (83%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           V A +Y+DP GG+ L+ VQL+E++GVWS  GP+ WEGCYYVYEVSVYHPST +I+ C VN
Sbjct: 229 VCACIYRDPVGGNALEIVQLEEVNGVWSTKGPKRWEGCYYVYEVSVYHPSTLRIDKCHVN 288

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RT LVNLD + LKP  WD L  EKPD+ SFSDISIYELH+RDFSA+D 
Sbjct: 289 DPYARGLSSDGRRTFLVNLDSDTLKPEEWDNLANEKPDILSFSDISIYELHIRDFSANDY 348

Query: 826 TVNPDFRGGFLAFT 867
           TV+P+FRGG+LAFT
Sbjct: 349 TVHPEFRGGYLAFT 362



 Score =  197 bits (502), Expect(2) = e-103
 Identities = 89/151 (58%), Positives = 120/151 (79%), Gaps = 1/151 (0%)
 Frame = +3

Query: 24  QDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPGPCG 203
           QD L ++RAYWV++S IAWN D  + S +L+AS+ A L +  +GVEG++++ +LE   CG
Sbjct: 82  QDSLLFSRAYWVSKSTIAWNVDVGDGSCFLFASKTAALSIVDDGVEGKDMQIKLEEDSCG 141

Query: 204 LPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGV-GKCMNATGLQLPGVLDDM 380
           LPENV++KFPHI+DY+AF VP ++D   L+KCQLAV +F   GKC+N TGLQLPGVLDD+
Sbjct: 142 LPENVIRKFPHIQDYRAFKVPSTLDAKTLLKCQLAVATFNSDGKCINVTGLQLPGVLDDL 201

Query: 381 FAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 473
           F+Y GPLGA++SEE+++LYLWAPTAQ  C C
Sbjct: 202 FSYNGPLGALYSEESVSLYLWAPTAQAVCAC 232


>ref|XP_021295293.1| pullulanase 1, chloroplastic isoform X1 [Herrania umbratica]
          Length = 965

 Score =  219 bits (557), Expect(2) = e-102
 Identities = 98/134 (73%), Positives = 112/134 (83%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           VHAH+YKDP GG  L+ +QL E +GVWS  GP+ WEGCYYVYEVSVYHPST QIE C  N
Sbjct: 223 VHAHIYKDPVGGSPLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYAN 282

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RTL VNLD   LKP GWDEL ++KPD+ SFSDISIYELH+RDFSA+D+
Sbjct: 283 DPYARGLSSDGRRTLFVNLDSNSLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDK 342

Query: 826 TVNPDFRGGFLAFT 867
           TVNPDF GG++AFT
Sbjct: 343 TVNPDFCGGYMAFT 356



 Score =  184 bits (466), Expect(2) = e-102
 Identities = 85/146 (58%), Positives = 114/146 (78%), Gaps = 1/146 (0%)
 Frame = +3

Query: 24  QDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPGPCG 203
           QD L Y+RAYWV+++IIAWN D  + S +LYAS+ A L VT +G++G + + +LE    G
Sbjct: 76  QDSLLYSRAYWVSKTIIAWNVDVGDGSCYLYASKVAALSVTDDGIQGLDAEIKLEEDSTG 135

Query: 204 LPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGV-GKCMNATGLQLPGVLDDM 380
           LP NV++KFPHI++Y+AF VP ++D   L+KCQLAV +F   GKC NATGLQLPG+LD++
Sbjct: 136 LPPNVIEKFPHIQNYRAFKVPPALDTKSLLKCQLAVAAFNSHGKCSNATGLQLPGILDEL 195

Query: 381 FAYGGPLGAVFSEEAITLYLWAPTAQ 458
           F+Y GPLGA + EEA++LYLWAPTAQ
Sbjct: 196 FSYDGPLGAFYLEEAVSLYLWAPTAQ 221


>ref|XP_021295296.1| pullulanase 1, chloroplastic isoform X3 [Herrania umbratica]
          Length = 877

 Score =  219 bits (557), Expect(2) = e-102
 Identities = 98/134 (73%), Positives = 112/134 (83%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           VHAH+YKDP GG  L+ +QL E +GVWS  GP+ WEGCYYVYEVSVYHPST QIE C  N
Sbjct: 223 VHAHIYKDPVGGSPLEIIQLVETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYAN 282

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+SSDG RTL VNLD   LKP GWDEL ++KPD+ SFSDISIYELH+RDFSA+D+
Sbjct: 283 DPYARGLSSDGRRTLFVNLDSNSLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDK 342

Query: 826 TVNPDFRGGFLAFT 867
           TVNPDF GG++AFT
Sbjct: 343 TVNPDFCGGYMAFT 356



 Score =  184 bits (466), Expect(2) = e-102
 Identities = 85/146 (58%), Positives = 114/146 (78%), Gaps = 1/146 (0%)
 Frame = +3

Query: 24  QDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPGPCG 203
           QD L Y+RAYWV+++IIAWN D  + S +LYAS+ A L VT +G++G + + +LE    G
Sbjct: 76  QDSLLYSRAYWVSKTIIAWNVDVGDGSCYLYASKVAALSVTDDGIQGLDAEIKLEEDSTG 135

Query: 204 LPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGV-GKCMNATGLQLPGVLDDM 380
           LP NV++KFPHI++Y+AF VP ++D   L+KCQLAV +F   GKC NATGLQLPG+LD++
Sbjct: 136 LPPNVIEKFPHIQNYRAFKVPPALDTKSLLKCQLAVAAFNSHGKCSNATGLQLPGILDEL 195

Query: 381 FAYGGPLGAVFSEEAITLYLWAPTAQ 458
           F+Y GPLGA + EEA++LYLWAPTAQ
Sbjct: 196 FSYDGPLGAFYLEEAVSLYLWAPTAQ 221


>ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Vitis vinifera]
          Length = 954

 Score =  218 bits (554), Expect(2) = e-102
 Identities = 101/135 (74%), Positives = 113/135 (83%)
 Frame = +1

Query: 463 VVHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKV 642
           VVHA +Y DPSGGD L+ V L+E +GVWSA GP+SWEGCYY YEVSVYHPST QIE C  
Sbjct: 217 VVHACIYGDPSGGDPLEIVCLEEFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTA 276

Query: 643 NDPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASD 822
           NDPYARG+S+D  RTLLVNLD + LKP GWD L +EKP L SFSDISIYELH+RDFSASD
Sbjct: 277 NDPYARGLSADARRTLLVNLDSDDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASD 336

Query: 823 ETVNPDFRGGFLAFT 867
            TV+PDFRGG+LAFT
Sbjct: 337 HTVHPDFRGGYLAFT 351



 Score =  184 bits (467), Expect(2) = e-102
 Identities = 89/157 (56%), Positives = 114/157 (72%), Gaps = 1/157 (0%)
 Frame = +3

Query: 6   VEESLSQDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQL 185
           V  S +QD L Y+RAYWV+ SIIAWN D  + S +LY+S+ A L +   G+ G ++  QL
Sbjct: 65  VSTSTAQDSLLYSRAYWVSESIIAWNVDVGDGSCYLYSSKIAALSIGDSGITGHDMTIQL 124

Query: 186 EPGPCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSFGV-GKCMNATGLQLP 362
           E    GLP NV++KFP ++ YKAF VP +VD   L+KCQLAV +F   G+C N TGLQLP
Sbjct: 125 EEDNGGLPINVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNVTGLQLP 184

Query: 363 GVLDDMFAYGGPLGAVFSEEAITLYLWAPTAQVGCTC 473
           GVLD++F+Y GPLGA+FS+EA+TLYLWAPTAQV   C
Sbjct: 185 GVLDELFSYEGPLGAIFSKEAVTLYLWAPTAQVVHAC 221


>ref|XP_016685975.1| PREDICTED: pullulanase 1, chloroplastic-like isoform X1 [Gossypium
           hirsutum]
          Length = 956

 Score =  211 bits (537), Expect(2) = e-102
 Identities = 93/134 (69%), Positives = 111/134 (82%)
 Frame = +1

Query: 466 VHAHVYKDPSGGDELDSVQLQELSGVWSAVGPRSWEGCYYVYEVSVYHPSTSQIETCKVN 645
           V+AH+YKDP GG  L+ + L+E +GVWS  GP+SWEGCYY+YEVSVYHPST  IE C  N
Sbjct: 220 VYAHIYKDPVGGSPLEIIPLEETNGVWSTKGPKSWEGCYYMYEVSVYHPSTLHIEKCYAN 279

Query: 646 DPYARGISSDGMRTLLVNLDCEQLKPTGWDELTEEKPDLRSFSDISIYELHVRDFSASDE 825
           DPYARG+S+DG RTL VNL  + LKP GWDEL + KPD+ SFSDISIYELH+RDFSA+D+
Sbjct: 280 DPYARGLSADGKRTLFVNLHADDLKPEGWDELADMKPDILSFSDISIYELHIRDFSANDD 339

Query: 826 TVNPDFRGGFLAFT 867
           TVN DFRGG++AFT
Sbjct: 340 TVNADFRGGYMAFT 353



 Score =  189 bits (479), Expect(2) = e-102
 Identities = 91/149 (61%), Positives = 115/149 (77%), Gaps = 1/149 (0%)
 Frame = +3

Query: 15  SLSQDCLSYARAYWVTRSIIAWNADDDETSVWLYASRKAQLFVTSEGVEGQEVKFQLEPG 194
           SLSQD L Y+RAYWVT++IIAWN D  E S +LYAS+ A L VT  G++G +++ +LE  
Sbjct: 70  SLSQDSLLYSRAYWVTKTIIAWNVDVVEGSCYLYASKVAALSVTDNGIQGHDLEIKLEED 129

Query: 195 PCGLPENVVQKFPHIKDYKAFHVPHSVDVGHLVKCQLAVVSF-GVGKCMNATGLQLPGVL 371
             GLP NV++KFPHI+DY+AF +P  +D  +LVKCQLAV  F   GKC NATGLQLPGVL
Sbjct: 130 RSGLPPNVIEKFPHIRDYRAFKLPPVLDAKNLVKCQLAVAEFNSQGKCRNATGLQLPGVL 189

Query: 372 DDMFAYGGPLGAVFSEEAITLYLWAPTAQ 458
           D++F+Y GPLGA +S EA++L LWAPTAQ
Sbjct: 190 DELFSYDGPLGAFYSAEAVSLCLWAPTAQ 218