BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon24_contig00016096
         (823 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020271372.1| DNA topoisomerase 6 subunit B [Asparagus off...   439   e-148
gb|PKI60225.1| hypothetical protein CRG98_019413 [Punica granatum]    423   e-143
ref|XP_008782884.1| PREDICTED: DNA topoisomerase 6 subunit B [Ph...   425   e-142
ref|XP_010257405.1| PREDICTED: DNA topoisomerase 6 subunit B iso...   425   e-142
ref|XP_010257404.1| PREDICTED: DNA topoisomerase 6 subunit B iso...   425   e-142
ref|XP_010928837.1| PREDICTED: DNA topoisomerase 6 subunit B [El...   424   e-142
gb|OWM73475.1| hypothetical protein CDL15_Pgr026574 [Punica gran...   423   e-142
ref|XP_016900692.1| PREDICTED: DNA topoisomerase 6 subunit B iso...   417   e-141
gb|OVA06658.1| Histidine kinase-like ATPase [Macleaya cordata]        420   e-141
ref|XP_008449756.1| PREDICTED: DNA topoisomerase 6 subunit B iso...   417   e-140
gb|PIA53423.1| hypothetical protein AQUCO_00900182v1 [Aquilegia ...   417   e-139
gb|PIA53424.1| hypothetical protein AQUCO_00900182v1 [Aquilegia ...   417   e-139
ref|XP_022153812.1| DNA topoisomerase 6 subunit B isoform X2 [Mo...   417   e-139
ref|XP_022153811.1| DNA topoisomerase 6 subunit B isoform X1 [Mo...   417   e-139
ref|XP_011653575.1| PREDICTED: DNA topoisomerase 6 subunit B [Cu...   417   e-139
ref|XP_008449755.1| PREDICTED: DNA topoisomerase 6 subunit B iso...   417   e-139
ref|XP_020541451.1| DNA topoisomerase 6 subunit B [Jatropha curcas]   416   e-139
gb|OMO72293.1| hypothetical protein CCACVL1_17863 [Corchorus cap...   412   e-139
ref|XP_023532908.1| DNA topoisomerase 6 subunit B isoform X1 [Cu...   415   e-139
ref|XP_020103516.1| DNA topoisomerase 6 subunit B [Ananas comosu...   415   e-139

>ref|XP_020271372.1| DNA topoisomerase 6 subunit B [Asparagus officinalis]
 gb|ONK79032.1| uncharacterized protein A4U43_C01F2170 [Asparagus officinalis]
          Length = 672

 Score =  439 bits (1128), Expect = e-148
 Identities = 224/273 (82%), Positives = 228/273 (83%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           MD +GSS S E PKKGRSKA PKK KESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR
Sbjct: 1   MDFNGSSGSPEEPKKGRSKA-PKKTKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FNTMIGL+DRERVDE LYDDFESAKAREKR
Sbjct: 60  ELVENSLDSAESISELPVIEITIEEISKSKFNTMIGLIDRERVDEELYDDFESAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EP S KG KGRGEASFFRVTCKDNGRGMP+DDIPNMFG
Sbjct: 120 LAKEARNQEIQAKNAALGKKLKEPASSKGAKGRGEASFFRVTCKDNGRGMPYDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPHVHLHEKRENK+EWHGAEIQVIIEGNWTTYR
Sbjct: 240 IPHVHLHEKRENKSEWHGAEIQVIIEGNWTTYR 272


>gb|PKI60225.1| hypothetical protein CRG98_019413 [Punica granatum]
          Length = 573

 Score =  423 bits (1087), Expect = e-143
 Identities = 212/273 (77%), Positives = 225/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           M+  GSSESS  PKKG+SK   +K KESVLKQKSPAEFFAENKNIAGFDNPGK LYTTVR
Sbjct: 1   METGGSSESSVEPKKGKSKT-RRKPKESVLKQKSPAEFFAENKNIAGFDNPGKCLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVEN+LDSA                    FN+MIGLVDRERVDEALYDD+ESAKAREKR
Sbjct: 60  ELVENALDSAESISELPVVEITIEEIERSEFNSMIGLVDRERVDEALYDDYESAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EPT VKGIKGRGEASF+RVTCKDNG+GMPHDDIPNMFG
Sbjct: 120 LAKEARNQEIQAKNALLGKKTKEPTPVKGIKGRGEASFYRVTCKDNGKGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSMK QNYISFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKAQNYISFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPH+HLHEK+ENK  WHGA+IQV+IEGNWTTYR
Sbjct: 240 IPHIHLHEKQENKERWHGAKIQVVIEGNWTTYR 272


>ref|XP_008782884.1| PREDICTED: DNA topoisomerase 6 subunit B [Phoenix dactylifera]
          Length = 676

 Score =  425 bits (1093), Expect = e-142
 Identities = 217/276 (78%), Positives = 228/276 (82%), Gaps = 3/276 (1%)
 Frame = +1

Query: 4   MDASGSSESSEAPKK---GRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYT 174
           M+A+GSSES E PKK   G+SK  P+KAKES+L QKSPAEFFAENKNIAGFDNPGKSLYT
Sbjct: 1   MEANGSSESLEVPKKAKRGKSKT-PQKAKESLLTQKSPAEFFAENKNIAGFDNPGKSLYT 59

Query: 175 TVRELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAR 354
           TVRELVENSLDSA                    FNTMIGL DRERVDE LYDDFESAKAR
Sbjct: 60  TVRELVENSLDSAESISELPDIEITIEEINKSKFNTMIGLADRERVDEELYDDFESAKAR 119

Query: 355 EKRLAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPN 534
           EKRLAKE R QE             EP+ V+G+KGRGEASFFRVTCKDNGRGMPHDDIPN
Sbjct: 120 EKRLAKEARFQEIQAKNAALGKKVKEPSVVRGLKGRGEASFFRVTCKDNGRGMPHDDIPN 179

Query: 535 MFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDI 714
           MFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIAS+M+GQNY SFCRLDIDI
Sbjct: 180 MFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASAMRGQNYTSFCRLDIDI 239

Query: 715 HRNIPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           HRNIPHVHLHEKRENK+ WHGAEIQVIIEGNWTTYR
Sbjct: 240 HRNIPHVHLHEKRENKDRWHGAEIQVIIEGNWTTYR 275


>ref|XP_010257405.1| PREDICTED: DNA topoisomerase 6 subunit B isoform X2 [Nelumbo
           nucifera]
          Length = 674

 Score =  425 bits (1092), Expect = e-142
 Identities = 212/273 (77%), Positives = 225/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           M+  GSSES E PKKG+S+A  KKAKESVLKQKSPAEFFAENKNIAGFDNPGK LYTTVR
Sbjct: 1   MEVGGSSESPEEPKKGKSRAT-KKAKESVLKQKSPAEFFAENKNIAGFDNPGKCLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FN+MIGL+DRERVDE LYDD+ESAKAREKR
Sbjct: 60  ELVENSLDSAESISELPVIEVTIEEIGKSRFNSMIGLIDRERVDEELYDDYESAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EP + KG KGRGEASFFRVTCKDNGRGMPHDDIPNMFG
Sbjct: 120 LAKEARIQEMQAKNAALRKKVKEPAAPKGTKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSM+GQNY SFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMRGQNYTSFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPH+HLHEKR+NK +WHGAEIQV+IEGNWTTYR
Sbjct: 240 IPHIHLHEKRDNKEKWHGAEIQVVIEGNWTTYR 272


>ref|XP_010257404.1| PREDICTED: DNA topoisomerase 6 subunit B isoform X1 [Nelumbo
           nucifera]
          Length = 675

 Score =  425 bits (1092), Expect = e-142
 Identities = 212/273 (77%), Positives = 225/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           M+  GSSES E PKKG+S+A  KKAKESVLKQKSPAEFFAENKNIAGFDNPGK LYTTVR
Sbjct: 1   MEVGGSSESPEEPKKGKSRAT-KKAKESVLKQKSPAEFFAENKNIAGFDNPGKCLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FN+MIGL+DRERVDE LYDD+ESAKAREKR
Sbjct: 60  ELVENSLDSAESISELPVIEVTIEEIGKSRFNSMIGLIDRERVDEELYDDYESAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EP + KG KGRGEASFFRVTCKDNGRGMPHDDIPNMFG
Sbjct: 120 LAKEARIQEMQAKNAALRKKVKEPAAPKGTKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSM+GQNY SFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMRGQNYTSFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPH+HLHEKR+NK +WHGAEIQV+IEGNWTTYR
Sbjct: 240 IPHIHLHEKRDNKEKWHGAEIQVVIEGNWTTYR 272


>ref|XP_010928837.1| PREDICTED: DNA topoisomerase 6 subunit B [Elaeis guineensis]
          Length = 677

 Score =  424 bits (1090), Expect = e-142
 Identities = 217/276 (78%), Positives = 225/276 (81%), Gaps = 3/276 (1%)
 Frame = +1

Query: 4   MDASGSSESSEAPKK---GRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYT 174
           MDA+GSSES E PKK   G+SK   KKAKESVL QKSPAEFFAENKNIAGFDNPGKSLYT
Sbjct: 1   MDANGSSESPEEPKKAKRGKSKTPQKKAKESVLTQKSPAEFFAENKNIAGFDNPGKSLYT 60

Query: 175 TVRELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAR 354
           TVRELVENSLDSA                    FNTMIGL D ERVDE LYDDFES KAR
Sbjct: 61  TVRELVENSLDSAESISELPNIEITIEEINKSKFNTMIGLGDHERVDEELYDDFESTKAR 120

Query: 355 EKRLAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPN 534
           EKRLAKE R QE             EP+ +KG KGRGEASFFRVTCKDNGRGMPHDDIPN
Sbjct: 121 EKRLAKEARFQETQAKNAALGKKVKEPSVMKGPKGRGEASFFRVTCKDNGRGMPHDDIPN 180

Query: 535 MFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDI 714
           MFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIAS+M+GQNYISFCRLDIDI
Sbjct: 181 MFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASAMRGQNYISFCRLDIDI 240

Query: 715 HRNIPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           HRNIPHVHLHEKREN ++WHGAEIQVIIEGNWTTYR
Sbjct: 241 HRNIPHVHLHEKRENMDQWHGAEIQVIIEGNWTTYR 276


>gb|OWM73475.1| hypothetical protein CDL15_Pgr026574 [Punica granatum]
          Length = 673

 Score =  423 bits (1087), Expect = e-142
 Identities = 212/273 (77%), Positives = 225/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           M+  GSSESS  PKKG+SK   +K KESVLKQKSPAEFFAENKNIAGFDNPGK LYTTVR
Sbjct: 1   METGGSSESSVEPKKGKSKT-RRKPKESVLKQKSPAEFFAENKNIAGFDNPGKCLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVEN+LDSA                    FN+MIGLVDRERVDEALYDD+ESAKAREKR
Sbjct: 60  ELVENALDSAESISELPVVEITIEEIERSEFNSMIGLVDRERVDEALYDDYESAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EPT VKGIKGRGEASF+RVTCKDNG+GMPHDDIPNMFG
Sbjct: 120 LAKEARNQEIQAKNALLGKKTKEPTPVKGIKGRGEASFYRVTCKDNGKGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSMK QNYISFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKAQNYISFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPH+HLHEK+ENK  WHGA+IQV+IEGNWTTYR
Sbjct: 240 IPHIHLHEKQENKERWHGAKIQVVIEGNWTTYR 272


>ref|XP_016900692.1| PREDICTED: DNA topoisomerase 6 subunit B isoform X3 [Cucumis melo]
          Length = 547

 Score =  417 bits (1071), Expect = e-141
 Identities = 206/273 (75%), Positives = 224/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           M+A G SES + PKKG+SK  P+K KES+LKQKSPAEFFAENKNIAGFDNPGK LYTTVR
Sbjct: 1   MEAGGGSESPDEPKKGKSKT-PRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FN+MIGLVDRERVDE LYDD+E+AKAREKR
Sbjct: 60  ELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EP + KG+KGRGEASF+RVTCKDNG+GMPHDDIPNMFG
Sbjct: 120 LAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSMKGQNY SFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPH+H+ EKR+NK+ WHGAEIQV+IEGNWTTYR
Sbjct: 240 IPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYR 272


>gb|OVA06658.1| Histidine kinase-like ATPase [Macleaya cordata]
          Length = 674

 Score =  420 bits (1080), Expect = e-141
 Identities = 211/269 (78%), Positives = 222/269 (82%)
 Frame = +1

Query: 16  GSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVE 195
           GSSES    KKG+SK  PKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVE
Sbjct: 6   GSSESPIETKKGKSKT-PKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVE 64

Query: 196 NSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKRLAKE 375
           NSLDSA                    FN+MIGLVDRERVDEALYDDFE+ KAREKRLAKE
Sbjct: 65  NSLDSAESISELPIIEVTIEEIGKSKFNSMIGLVDRERVDEALYDDFETVKAREKRLAKE 124

Query: 376 VRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFGRVLS 555
            R QE             EP  +KGIKGRGEASFFRVTCKDNG+GM HDDIPNMFGRVLS
Sbjct: 125 ARDQENQAKNAALGKKVKEPAPLKGIKGRGEASFFRVTCKDNGKGMAHDDIPNMFGRVLS 184

Query: 556 GTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRNIPHV 735
           GTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSM+GQNY+SFCRLDIDIHRNIPH+
Sbjct: 185 GTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMRGQNYVSFCRLDIDIHRNIPHI 244

Query: 736 HLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           HLHEKR+NK  WHGAEIQV+IEGNWTTYR
Sbjct: 245 HLHEKRDNKERWHGAEIQVVIEGNWTTYR 273


>ref|XP_008449756.1| PREDICTED: DNA topoisomerase 6 subunit B isoform X2 [Cucumis melo]
          Length = 612

 Score =  417 bits (1071), Expect = e-140
 Identities = 206/273 (75%), Positives = 224/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           M+A G SES + PKKG+SK  P+K KES+LKQKSPAEFFAENKNIAGFDNPGK LYTTVR
Sbjct: 1   MEAGGGSESPDEPKKGKSKT-PRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FN+MIGLVDRERVDE LYDD+E+AKAREKR
Sbjct: 60  ELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EP + KG+KGRGEASF+RVTCKDNG+GMPHDDIPNMFG
Sbjct: 120 LAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSMKGQNY SFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPH+H+ EKR+NK+ WHGAEIQV+IEGNWTTYR
Sbjct: 240 IPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYR 272


>gb|PIA53423.1| hypothetical protein AQUCO_00900182v1 [Aquilegia coerulea]
          Length = 675

 Score =  417 bits (1073), Expect = e-139
 Identities = 209/273 (76%), Positives = 224/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           MD  GSSES   PKK  +K  PKKAKESVLKQKSPAEFF+ENKNIAGFDNPGKSLYTTVR
Sbjct: 1   MDTGGSSESPIEPKKRNAKT-PKKAKESVLKQKSPAEFFSENKNIAGFDNPGKSLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FN+MIGLVD+ERVD+ALYDDFESAKAREKR
Sbjct: 60  ELVENSLDSAESISELPVIEITIEEIGKVKFNSMIGLVDQERVDQALYDDFESAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE + QE             EP++ KGIKGRGEASFFRVTCKDNG+GMPHDDIPNMFG
Sbjct: 120 LAKEAKNQEIQAKNASLGKKTKEPSAPKGIKGRGEASFFRVTCKDNGKGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGK+GLGAKMALIWSKMSTGLPIEI+SSMK Q+Y SFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKYGLGAKMALIWSKMSTGLPIEISSSMKNQSYTSFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPHVH+HEKR+NK  WHGAEIQV+IEGNWTTYR
Sbjct: 240 IPHVHIHEKRDNKERWHGAEIQVVIEGNWTTYR 272


>gb|PIA53424.1| hypothetical protein AQUCO_00900182v1 [Aquilegia coerulea]
          Length = 677

 Score =  417 bits (1073), Expect = e-139
 Identities = 209/273 (76%), Positives = 224/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           MD  GSSES   PKK  +K  PKKAKESVLKQKSPAEFF+ENKNIAGFDNPGKSLYTTVR
Sbjct: 1   MDTGGSSESPIEPKKRNAKT-PKKAKESVLKQKSPAEFFSENKNIAGFDNPGKSLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FN+MIGLVD+ERVD+ALYDDFESAKAREKR
Sbjct: 60  ELVENSLDSAESISELPVIEITIEEIGKVKFNSMIGLVDQERVDQALYDDFESAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE + QE             EP++ KGIKGRGEASFFRVTCKDNG+GMPHDDIPNMFG
Sbjct: 120 LAKEAKNQEIQAKNASLGKKTKEPSAPKGIKGRGEASFFRVTCKDNGKGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGK+GLGAKMALIWSKMSTGLPIEI+SSMK Q+Y SFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKYGLGAKMALIWSKMSTGLPIEISSSMKNQSYTSFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPHVH+HEKR+NK  WHGAEIQV+IEGNWTTYR
Sbjct: 240 IPHVHIHEKRDNKERWHGAEIQVVIEGNWTTYR 272


>ref|XP_022153812.1| DNA topoisomerase 6 subunit B isoform X2 [Momordica charantia]
          Length = 673

 Score =  417 bits (1072), Expect = e-139
 Identities = 207/273 (75%), Positives = 224/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           M+A G SES + PKKG+SK  PKK KES+LKQKSPAEFFAENKNIAGFDNPGK LYTTVR
Sbjct: 1   MEAGGGSESPDEPKKGKSKT-PKKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FN+MIGLVDRER+D ALYDD+E+AKAREKR
Sbjct: 60  ELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERIDAALYDDYETAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EP + KG+KGRGEASF+RVTCKDNGRGMPHDDIPNMFG
Sbjct: 120 LAKEARAQEIQAKNAALGKKVREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSMKGQNYISFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPH+H+ EK +NK+ WHGAEIQV+IEGNWTTYR
Sbjct: 240 IPHIHIQEKCDNKDLWHGAEIQVVIEGNWTTYR 272


>ref|XP_022153811.1| DNA topoisomerase 6 subunit B isoform X1 [Momordica charantia]
          Length = 683

 Score =  417 bits (1072), Expect = e-139
 Identities = 207/273 (75%), Positives = 224/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           M+A G SES + PKKG+SK  PKK KES+LKQKSPAEFFAENKNIAGFDNPGK LYTTVR
Sbjct: 1   MEAGGGSESPDEPKKGKSKT-PKKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FN+MIGLVDRER+D ALYDD+E+AKAREKR
Sbjct: 60  ELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERIDAALYDDYETAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EP + KG+KGRGEASF+RVTCKDNGRGMPHDDIPNMFG
Sbjct: 120 LAKEARAQEIQAKNAALGKKVREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSMKGQNYISFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPH+H+ EK +NK+ WHGAEIQV+IEGNWTTYR
Sbjct: 240 IPHIHIQEKCDNKDLWHGAEIQVVIEGNWTTYR 272


>ref|XP_011653575.1| PREDICTED: DNA topoisomerase 6 subunit B [Cucumis sativus]
          Length = 673

 Score =  417 bits (1071), Expect = e-139
 Identities = 206/273 (75%), Positives = 224/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           M+A G SES + PKKG+SK  P+K KES+LKQKSPAEFFAENKNIAGFDNPGK LYTTVR
Sbjct: 1   MEAGGGSESPDEPKKGKSKT-PRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FN+MIGLVDRERVDE LYDD+E+AKAREKR
Sbjct: 60  ELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EP + KG+KGRGEASF+RVTCKDNG+GMPHDDIPNMFG
Sbjct: 120 LAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSMKGQNY SFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPH+H+ EKR+NK+ WHGAEIQV+IEGNWTTYR
Sbjct: 240 IPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYR 272


>ref|XP_008449755.1| PREDICTED: DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo]
          Length = 673

 Score =  417 bits (1071), Expect = e-139
 Identities = 206/273 (75%), Positives = 224/273 (82%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           M+A G SES + PKKG+SK  P+K KES+LKQKSPAEFFAENKNIAGFDNPGK LYTTVR
Sbjct: 1   MEAGGGSESPDEPKKGKSKT-PRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FN+MIGLVDRERVDE LYDD+E+AKAREKR
Sbjct: 60  ELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EP + KG+KGRGEASF+RVTCKDNG+GMPHDDIPNMFG
Sbjct: 120 LAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSMKGQNY SFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPH+H+ EKR+NK+ WHGAEIQV+IEGNWTTYR
Sbjct: 240 IPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYR 272


>ref|XP_020541451.1| DNA topoisomerase 6 subunit B [Jatropha curcas]
          Length = 674

 Score =  416 bits (1070), Expect = e-139
 Identities = 207/269 (76%), Positives = 222/269 (82%)
 Frame = +1

Query: 16  GSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVE 195
           GSSES   PKKG+SK  P+K KES+LKQKSPAEFFAENKNIAGFDNPGK LYTTVRELVE
Sbjct: 6   GSSESPTEPKKGKSKT-PRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVE 64

Query: 196 NSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKRLAKE 375
           NSLDSA                    FN+MIGLVDRERVDE LYDD+E+AKAREKRLAKE
Sbjct: 65  NSLDSAESISELPVVEITLEEIGKSKFNSMIGLVDRERVDEELYDDYETAKAREKRLAKE 124

Query: 376 VRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFGRVLS 555
            R QE             E ++ KG+KGRGEASF+RVTCKDNGRGMPHDDIPNMFGRVLS
Sbjct: 125 ARAQEIQAKNAALGKKVREQSAAKGVKGRGEASFYRVTCKDNGRGMPHDDIPNMFGRVLS 184

Query: 556 GTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRNIPHV 735
           GTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSMKGQNY+SFCRLDIDIHRNIPHV
Sbjct: 185 GTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYVSFCRLDIDIHRNIPHV 244

Query: 736 HLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           HLHEKR+N + WHGAEIQV+IEGNWTTYR
Sbjct: 245 HLHEKRKNDDGWHGAEIQVVIEGNWTTYR 273


>gb|OMO72293.1| hypothetical protein CCACVL1_17863 [Corchorus capsularis]
          Length = 563

 Score =  412 bits (1058), Expect = e-139
 Identities = 207/267 (77%), Positives = 218/267 (81%)
 Frame = +1

Query: 22  SESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENS 201
           SES    KKGRSK  PKKAKES+LKQKSPAEFFAENKNIAGFDNPGK LYTTVRELVEN+
Sbjct: 3   SESPIETKKGRSKT-PKKAKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENA 61

Query: 202 LDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKRLAKEVR 381
           LDSA                    FN+MIGLVDRERVD  LYDDFE+AKAREKRLAKE R
Sbjct: 62  LDSAESISELPVVEITIEEIVKSKFNSMIGLVDRERVDAELYDDFETAKAREKRLAKEAR 121

Query: 382 TQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFGRVLSGT 561
            QE             E  + KG+KGRGEASFFRVTCKDNGRGMPHDDIPNMFGRVLSGT
Sbjct: 122 AQELQAKNAALGKKVKESAAPKGVKGRGEASFFRVTCKDNGRGMPHDDIPNMFGRVLSGT 181

Query: 562 KYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRNIPHVHL 741
           KYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSMK QNY+SFCRLDIDIHRNIPHVHL
Sbjct: 182 KYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQNYVSFCRLDIDIHRNIPHVHL 241

Query: 742 HEKRENKNEWHGAEIQVIIEGNWTTYR 822
           HEKR+NK+ WHGAEIQV+IEGNWTTYR
Sbjct: 242 HEKRDNKDRWHGAEIQVVIEGNWTTYR 268


>ref|XP_023532908.1| DNA topoisomerase 6 subunit B isoform X1 [Cucurbita pepo subsp.
           pepo]
          Length = 673

 Score =  415 bits (1067), Expect = e-139
 Identities = 206/273 (75%), Positives = 223/273 (81%)
 Frame = +1

Query: 4   MDASGSSESSEAPKKGRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 183
           M+  G SES + PKKG+SK  P+K KES+LKQKSPAEFFAENKNIAGFDNPGKSLYTTVR
Sbjct: 1   MEIGGGSESPDEPKKGKSKT-PRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVR 59

Query: 184 ELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKAREKR 363
           ELVENSLDSA                    FN+MIGLVDRERVD  LYDD+E+AKAREKR
Sbjct: 60  ELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKR 119

Query: 364 LAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIPNMFG 543
           LAKE R QE             EP + KG+KGRGEASF+RVTCKDNGRGMPHDDIPNMFG
Sbjct: 120 LAKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFG 179

Query: 544 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDIDIHRN 723
           RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI+SSMKGQNY SFCRLDIDIHRN
Sbjct: 180 RVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRN 239

Query: 724 IPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IPH+H+ EKR+NK+ WHGAEIQV+IEGNWTTYR
Sbjct: 240 IPHIHVQEKRDNKDLWHGAEIQVVIEGNWTTYR 272


>ref|XP_020103516.1| DNA topoisomerase 6 subunit B [Ananas comosus]
 gb|OAY68721.1| DNA topoisomerase 6 subunit B [Ananas comosus]
          Length = 678

 Score =  415 bits (1067), Expect = e-139
 Identities = 211/277 (76%), Positives = 222/277 (80%), Gaps = 4/277 (1%)
 Frame = +1

Query: 4   MDASG-SSESSEAPKK---GRSKAVPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLY 171
           MD+ G      E PKK   G++K  PKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLY
Sbjct: 1   MDSEGIGGAPEEEPKKSNRGKAKQNPKKAKESVLKQKSPAEFFAENKNIAGFDNPGKSLY 60

Query: 172 TTVRELVENSLDSAXXXXXXXXXXXXXXXXXXXXFNTMIGLVDRERVDEALYDDFESAKA 351
           TTVRELVENSLDSA                    FNTMIGLVDRERVDE LYDDFESAKA
Sbjct: 61  TTVRELVENSLDSAESISELPVIEIAIEEINKSKFNTMIGLVDRERVDEELYDDFESAKA 120

Query: 352 REKRLAKEVRTQEXXXXXXXXXXXXXEPTSVKGIKGRGEASFFRVTCKDNGRGMPHDDIP 531
           REKRLAKE R QE             EP++ KG K RGEA+FFRVTCKDNGRGMPHDDIP
Sbjct: 121 REKRLAKEARVQEIQAKNIALGKKFKEPSTAKGAKSRGEAAFFRVTCKDNGRGMPHDDIP 180

Query: 532 NMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEIASSMKGQNYISFCRLDID 711
           NMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEI SSMKGQNY+S+CRLDID
Sbjct: 181 NMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEITSSMKGQNYVSYCRLDID 240

Query: 712 IHRNIPHVHLHEKRENKNEWHGAEIQVIIEGNWTTYR 822
           IH+NIPHVH HEKRENK+ WHGAEIQVIIEGNWTT+R
Sbjct: 241 IHKNIPHVHAHEKRENKDRWHGAEIQVIIEGNWTTHR 277