BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon24_contig00015212
         (1301 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_008783492.1| PREDICTED: S-adenosyl-L-methionine-dependent...   753   0.0  
ref|XP_008783491.1| PREDICTED: S-adenosyl-L-methionine-dependent...   750   0.0  
ref|XP_010942945.1| PREDICTED: S-adenosyl-L-methionine-dependent...   747   0.0  
dbj|BAF13343.1| Os03g0775300 [Oryza sativa Japonica Group] >gi|9...   733   0.0  
ref|NP_001347253.1| uncharacterized protein LOC111828519 [Oryza ...   724   0.0  
ref|XP_015631211.1| PREDICTED: S-adenosyl-L-methionine-dependent...   733   0.0  
ref|XP_020704408.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   731   0.0  
ref|XP_009399072.1| PREDICTED: S-adenosyl-L-methionine-dependent...   731   0.0  
ref|XP_020092095.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   728   0.0  
gb|OAY80972.1| S-adenosyl-L-methionine-dependent tRNA 4-demethyl...   728   0.0  
ref|NP_001347248.1| uncharacterized protein LOC100273754 [Zea ma...   726   0.0  
gb|KQK86877.1| hypothetical protein SETIT_034607mg [Setaria ital...   725   0.0  
ref|NP_001347259.1| uncharacterized LOC111828523 [Sorghum bicolor]    725   0.0  
ref|NP_001347566.1| uncharacterized protein LOC111946219 [Setari...   725   0.0  
ref|XP_020180383.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   721   0.0  
dbj|BAK06688.1| predicted protein [Hordeum vulgare subsp. vulgare]    719   0.0  
gb|KZV28868.1| S-adenosyl-L-methionine-dependent tRNA 4-demethyl...   718   0.0  
ref|XP_014752193.1| PREDICTED: S-adenosyl-L-methionine-dependent...   717   0.0  
ref|XP_017981378.1| PREDICTED: S-adenosyl-L-methionine-dependent...   717   0.0  
gb|EOY14757.1| Flavodoxin family protein / radical SAM domain-co...   717   0.0  

>ref|XP_008783492.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like isoform X1 [Phoenix dactylifera]
          Length = 648

 Score =  753 bits (1943), Expect = 0.0
 Identities = 357/409 (87%), Positives = 381/409 (93%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGSHSGVKIC 1122
            GKAPSRK   +L NG          NG+NG REMVTP+IR SL+KQGYKI+GSHSGVK+C
Sbjct: 244  GKAPSRKSTGALSNGGQ--------NGDNGLREMVTPIIRTSLEKQGYKIVGSHSGVKLC 295

Query: 1121 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD 942
            RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD
Sbjct: 296  RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD 355

Query: 941  PLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDEL 762
            PLVIVNAAIDQHTKMIKQMKGVPGV QE+L EGLSP+HCALSLVGEPIMYPEIN+LIDEL
Sbjct: 356  PLVIVNAAIDQHTKMIKQMKGVPGVKQEQLLEGLSPKHCALSLVGEPIMYPEINSLIDEL 415

Query: 761  HRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLK 582
            H RHISTFLVTNAQFPD+IK L+P+TQLYVSVDAATKDSLKAIDRPLF DFWERFVDSLK
Sbjct: 416  HSRHISTFLVTNAQFPDRIKMLRPVTQLYVSVDAATKDSLKAIDRPLFSDFWERFVDSLK 475

Query: 581  ALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMENV 402
            ALRDKEQRTVYRLTLVKGWNAEDIDAYA+L  IGKPDLVE+KGVTYCGS+ATSKLTMENV
Sbjct: 476  ALRDKEQRTVYRLTLVKGWNAEDIDAYANLLGIGKPDLVEMKGVTYCGSSATSKLTMENV 535

Query: 401  PWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHELV 222
            PWHSDVR FSEALAIKS+G+YEVACEHVHSCCV+LAKV K+K+ GQW+TWIDYERFHELV
Sbjct: 536  PWHSDVRAFSEALAIKSSGEYEVACEHVHSCCVVLAKVSKFKVKGQWYTWIDYERFHELV 595

Query: 221  ASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIRAS 75
            ASGKPFKSE YMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAA+IR S
Sbjct: 596  ASGKPFKSEHYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAASIRGS 644


>ref|XP_008783491.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like isoform X2 [Phoenix dactylifera]
          Length = 648

 Score =  750 bits (1937), Expect = 0.0
 Identities = 356/409 (87%), Positives = 380/409 (92%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGSHSGVKIC 1122
            GKAPSRK   +L NG          NG+NG REMVTP+I  SL+KQGYKI+GSHSGVK+C
Sbjct: 244  GKAPSRKSTGALSNGGQ--------NGDNGLREMVTPIITTSLEKQGYKIVGSHSGVKLC 295

Query: 1121 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD 942
            RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD
Sbjct: 296  RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD 355

Query: 941  PLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDEL 762
            PLVIVNAAIDQHTKMIKQMKGVPGV QE+L EGLSP+HCALSLVGEPIMYPEIN+LIDEL
Sbjct: 356  PLVIVNAAIDQHTKMIKQMKGVPGVKQEQLLEGLSPKHCALSLVGEPIMYPEINSLIDEL 415

Query: 761  HRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLK 582
            H RHISTFLVTNAQFPD+IK L+P+TQLYVSVDAATKDSLKAIDRPLF DFWERFVDSLK
Sbjct: 416  HSRHISTFLVTNAQFPDRIKMLRPVTQLYVSVDAATKDSLKAIDRPLFSDFWERFVDSLK 475

Query: 581  ALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMENV 402
            ALRDKEQRTVYRLTLVKGWNAEDIDAYA+L  IGKPDLVE+KGVTYCGS+ATSKLTMENV
Sbjct: 476  ALRDKEQRTVYRLTLVKGWNAEDIDAYANLLGIGKPDLVEMKGVTYCGSSATSKLTMENV 535

Query: 401  PWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHELV 222
            PWHSDVR FSEALAIKS+G+YEVACEHVHSCCV+LAKV K+K+ GQW+TWIDYERFHELV
Sbjct: 536  PWHSDVRAFSEALAIKSSGEYEVACEHVHSCCVVLAKVSKFKVKGQWYTWIDYERFHELV 595

Query: 221  ASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIRAS 75
            ASGKPFKSE YMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAA+IR S
Sbjct: 596  ASGKPFKSEHYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAASIRGS 644


>ref|XP_010942945.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Elaeis guineensis]
          Length = 647

 Score =  747 bits (1928), Expect = 0.0
 Identities = 356/407 (87%), Positives = 377/407 (92%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGSHSGVKIC 1122
            GKAPSRK   +L NG          NG+NG REMVTP+IR SL+KQGYKIIGSHSGVK+C
Sbjct: 248  GKAPSRKSTGALSNGGQ--------NGDNGLREMVTPIIRTSLEKQGYKIIGSHSGVKLC 299

Query: 1121 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD 942
            RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD
Sbjct: 300  RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD 359

Query: 941  PLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDEL 762
            PLVIVNAAIDQHTKMIKQMKGVPGV QE+L EGLSPRHCALSLVGEPIMYPEIN+LIDEL
Sbjct: 360  PLVIVNAAIDQHTKMIKQMKGVPGVKQEQLLEGLSPRHCALSLVGEPIMYPEINSLIDEL 419

Query: 761  HRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLK 582
            H RHISTFLVTNAQFPD+IK L+ +TQLYVSVDAATKDSLKAIDRPLF DFWERFVDSLK
Sbjct: 420  HSRHISTFLVTNAQFPDRIKMLRSVTQLYVSVDAATKDSLKAIDRPLFSDFWERFVDSLK 479

Query: 581  ALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMENV 402
            AL+DKEQRTVYRLTLVKGWNAEDIDAYA+L  IGKPDLVEIKGVTYCGS+ATSKLTMENV
Sbjct: 480  ALQDKEQRTVYRLTLVKGWNAEDIDAYANLLGIGKPDLVEIKGVTYCGSSATSKLTMENV 539

Query: 401  PWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHELV 222
            PWHSDV+ FSEALA+KS+G+YEVACEHVHSCCVLLAK  K+K+ GQW TWIDYERFHELV
Sbjct: 540  PWHSDVKAFSEALAVKSSGEYEVACEHVHSCCVLLAKASKFKVKGQWFTWIDYERFHELV 599

Query: 221  ASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 81
            ASGKPFKSEDYMA+TPSWAVYGAEEGGFDPDQSR KKERRHGAA+IR
Sbjct: 600  ASGKPFKSEDYMALTPSWAVYGAEEGGFDPDQSRCKKERRHGAASIR 646


>dbj|BAF13343.1| Os03g0775300 [Oryza sativa Japonica Group]
 dbj|BAS86627.1| Os03g0775300 [Oryza sativa Japonica Group]
          Length = 600

 Score =  733 bits (1892), Expect = 0.0
 Identities = 344/408 (84%), Positives = 374/408 (91%), Gaps = 1/408 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GKAP+ +         NG  E +  NG ENG R+MVTP+IR SL+KQGYKIIGSHSGVKI
Sbjct: 199  GKAPAARR-------RNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKI 251

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD
Sbjct: 252  CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMD 311

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            DPL IVNAAIDQHTKM+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYPEIN LIDE
Sbjct: 312  DPLDIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDE 371

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LHRRHISTFLVTNAQFPDKIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSL
Sbjct: 372  LHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSL 431

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            K+L DK+QRTVYRLTLVKGWNAE+ID YA L  +G+PD +EIKGVTYCGS+ATSKLTMEN
Sbjct: 432  KSLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMEN 491

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWHSDV+DFSEALA+KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFHEL
Sbjct: 492  VPWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHEL 551

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 81
            V SGKPF+S+DYMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAAA++
Sbjct: 552  VTSGKPFRSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAAALK 599


>ref|NP_001347253.1| uncharacterized protein LOC111828519 [Oryza brachyantha]
          Length = 406

 Score =  724 bits (1869), Expect = 0.0
 Identities = 339/406 (83%), Positives = 372/406 (91%), Gaps = 1/406 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GKA +R+        +NG  E +  NG ENG R+MVTP+IRASL+KQGYKIIGSHSGVKI
Sbjct: 6    GKAQARR--------QNGKVEGALSNGGENGPRDMVTPIIRASLEKQGYKIIGSHSGVKI 57

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD
Sbjct: 58   CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMD 117

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            DPL IVN AIDQHTKM+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYPEIN L+ E
Sbjct: 118  DPLDIVNTAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLVGE 177

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LHRRHISTFLVTNAQFPDKIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSL
Sbjct: 178  LHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSL 237

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            K+L +K+QRTVYRLTLVKGWN E+IDAYA L ++G+PD +EIKGVTYCGS+ TSKLTMEN
Sbjct: 238  KSLHEKDQRTVYRLTLVKGWNVEEIDAYAKLLNLGQPDFIEIKGVTYCGSSTTSKLTMEN 297

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWHSDV++FSEALA+KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFH+L
Sbjct: 298  VPWHSDVKEFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHDL 357

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAA 87
            V SGKPFKS+DYMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAAA
Sbjct: 358  VTSGKPFKSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAAA 403


>ref|XP_015631211.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Oryza sativa Japonica Group]
 sp|Q8H8N3.1|TYW1_ORYSJ RecName: Full=S-adenosyl-L-methionine-dependent tRNA
            4-demethylwyosine synthase; AltName: Full=tRNA
            wybutosine-synthesizing protein 1 homolog
 gb|AAN65027.1| putative oxidoreductase [Oryza sativa Japonica Group]
 gb|ABF99122.1| radical S-adenosyl methionine and flavodoxin domains 1, isoform 1,
            putative, expressed [Oryza sativa Japonica Group]
 gb|EAY92018.1| hypothetical protein OsI_13711 [Oryza sativa Indica Group]
          Length = 653

 Score =  733 bits (1892), Expect = 0.0
 Identities = 344/408 (84%), Positives = 374/408 (91%), Gaps = 1/408 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GKAP+ +         NG  E +  NG ENG R+MVTP+IR SL+KQGYKIIGSHSGVKI
Sbjct: 252  GKAPAARR-------RNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKI 304

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD
Sbjct: 305  CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMD 364

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            DPL IVNAAIDQHTKM+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYPEIN LIDE
Sbjct: 365  DPLDIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDE 424

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LHRRHISTFLVTNAQFPDKIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSL
Sbjct: 425  LHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSL 484

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            K+L DK+QRTVYRLTLVKGWNAE+ID YA L  +G+PD +EIKGVTYCGS+ATSKLTMEN
Sbjct: 485  KSLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMEN 544

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWHSDV+DFSEALA+KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFHEL
Sbjct: 545  VPWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHEL 604

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 81
            V SGKPF+S+DYMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAAA++
Sbjct: 605  VTSGKPFRSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAAALK 652


>ref|XP_020704408.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Dendrobium catenatum]
          Length = 664

 Score =  731 bits (1888), Expect = 0.0
 Identities = 346/407 (85%), Positives = 375/407 (92%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGSHSGVKIC 1122
            GKAPSRK+ A LVNG NGV            +EMVTP+IR +L+KQGYKIIGSHSGVKIC
Sbjct: 252  GKAPSRKMKAELVNG-NGV-----------IKEMVTPIIRNNLEKQGYKIIGSHSGVKIC 299

Query: 1121 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD 942
            RWTK+QLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK WRWKMDD
Sbjct: 300  RWTKAQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKEWRWKMDD 359

Query: 941  PLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDEL 762
            PLVIV+AAIDQHTKMIKQMKGVPGV +++  EGLSPRHCALSLVGEPIMYPEIN LI+EL
Sbjct: 360  PLVIVDAAIDQHTKMIKQMKGVPGVKEQQFLEGLSPRHCALSLVGEPIMYPEINRLIEEL 419

Query: 761  HRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLK 582
            HRRHISTFLVTNAQFPD+IK LKPITQLYVSVDAATK+SLKA+DRPLFGDFWERFVDSLK
Sbjct: 420  HRRHISTFLVTNAQFPDRIKMLKPITQLYVSVDAATKESLKAVDRPLFGDFWERFVDSLK 479

Query: 581  ALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMENV 402
            AL DK+QRTVYRLTLVKGWNAE++DAYA+L  +G+PD +EIKGVTYCGSTATSKLTMENV
Sbjct: 480  ALHDKQQRTVYRLTLVKGWNAEEVDAYANLLGLGEPDFIEIKGVTYCGSTATSKLTMENV 539

Query: 401  PWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHELV 222
            PWHSDV++FSE LAIKSNG YEVACEHVHSCCVLLAKVDK+KINGQWHTWIDYERFH+LV
Sbjct: 540  PWHSDVKEFSETLAIKSNGLYEVACEHVHSCCVLLAKVDKFKINGQWHTWIDYERFHDLV 599

Query: 221  ASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 81
            ASGKPF +EDYMAVTPSWAVYGAEEGGFDP Q R+KKERRHG AA++
Sbjct: 600  ASGKPFGTEDYMAVTPSWAVYGAEEGGFDPKQFRYKKERRHGPAALK 646


>ref|XP_009399072.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Musa acuminata subsp. malaccensis]
          Length = 647

 Score =  731 bits (1886), Expect = 0.0
 Identities = 344/407 (84%), Positives = 377/407 (92%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGSHSGVKIC 1122
            GKAPSR+   +L NG            ENG ++MVTPVIR +L+KQGYKIIGSHSGVK+C
Sbjct: 251  GKAPSRRPSVALTNG-----------WENGVKDMVTPVIRVNLEKQGYKIIGSHSGVKLC 299

Query: 1121 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD 942
            RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD+
Sbjct: 300  RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDN 359

Query: 941  PLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDEL 762
            PL IV+ AID+HTKMIKQMKGVPGV  ERLSEGLSPRHCALSLVGEPIMYPEINTL+DEL
Sbjct: 360  PLEIVDVAIDEHTKMIKQMKGVPGVKPERLSEGLSPRHCALSLVGEPIMYPEINTLVDEL 419

Query: 761  HRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLK 582
            HRRHISTFLVTNAQFPD+IK LKPITQLYVSVDAATKDSLKAIDRPLFGDFWERF+DSLK
Sbjct: 420  HRRHISTFLVTNAQFPDRIKMLKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFLDSLK 479

Query: 581  ALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMENV 402
            AL+DKEQRTVYRLTLVKGWNAED++AYA+L  IGKPDLVEIKGVTYCG++ATSKLTM+NV
Sbjct: 480  ALQDKEQRTVYRLTLVKGWNAEDVEAYANLLGIGKPDLVEIKGVTYCGTSATSKLTMDNV 539

Query: 401  PWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHELV 222
            PWHSDV+ FSEALA+KS+G+YEVACEH HSCCVLLAKVDK+KI G+W+TWIDYERFHELV
Sbjct: 540  PWHSDVKAFSEALAMKSSGEYEVACEHAHSCCVLLAKVDKFKIKGEWYTWIDYERFHELV 599

Query: 221  ASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 81
            ASGK FK+EDYMA TPSWAVYGAEEGGFDPDQSR++KERRHGAA+++
Sbjct: 600  ASGKSFKTEDYMARTPSWAVYGAEEGGFDPDQSRYRKERRHGAASVQ 646


>ref|XP_020092095.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Ananas comosus]
          Length = 655

 Score =  728 bits (1879), Expect = 0.0
 Identities = 346/404 (85%), Positives = 377/404 (93%), Gaps = 1/404 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNGE-NGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GKAPSRK   +LVNG++     + VNG+ +G REMVTP+IR SL+KQGYKIIGSHSGVK+
Sbjct: 253  GKAPSRKSSVTLVNGKSS---GTLVNGKGDGLREMVTPIIRTSLEKQGYKIIGSHSGVKL 309

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKH+FYGIESHRCMEATPSLACANKCVFCWRHH NPVGKSWRWKMD
Sbjct: 310  CRWTKSQLRGRGGCYKHAFYGIESHRCMEATPSLACANKCVFCWRHHANPVGKSWRWKMD 369

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            DPL IV+AAIDQHTKMIKQMKGVPGV QERL EGLSPRHCALSLVGEPIMYPEINTLIDE
Sbjct: 370  DPLEIVDAAIDQHTKMIKQMKGVPGVKQERLDEGLSPRHCALSLVGEPIMYPEINTLIDE 429

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LH+R ISTFLVTNAQFPD+IK LKPITQLYVSVDA+TK+SLKAIDRPLFGDFWERF+DSL
Sbjct: 430  LHQRRISTFLVTNAQFPDRIKMLKPITQLYVSVDASTKESLKAIDRPLFGDFWERFLDSL 489

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            KAL DKEQRTVYRLTLVKGWNAE+IDAYA+L  +GKPDLVEIKGVTYCGS+ATSKLTMEN
Sbjct: 490  KALHDKEQRTVYRLTLVKGWNAEEIDAYANLLTMGKPDLVEIKGVTYCGSSATSKLTMEN 549

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWHSDV+ FSEALA+KSNG YEVACEHVHSCCVLLAKVDK+K++G+W+TWIDYERF EL
Sbjct: 550  VPWHSDVKAFSEALAVKSNGIYEVACEHVHSCCVLLAKVDKFKVDGKWYTWIDYERFQEL 609

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGA 93
            VASG PF++EDY A+TPSWAVYGAEEGGFDP QSR+KKERRHGA
Sbjct: 610  VASGTPFRTEDYTALTPSWAVYGAEEGGFDPGQSRYKKERRHGA 653


>gb|OAY80972.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Ananas comosus]
          Length = 663

 Score =  728 bits (1879), Expect = 0.0
 Identities = 346/404 (85%), Positives = 377/404 (93%), Gaps = 1/404 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNGE-NGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GKAPSRK   +LVNG++     + VNG+ +G REMVTP+IR SL+KQGYKIIGSHSGVK+
Sbjct: 261  GKAPSRKSSVTLVNGKSS---GTLVNGKGDGLREMVTPIIRTSLEKQGYKIIGSHSGVKL 317

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKH+FYGIESHRCMEATPSLACANKCVFCWRHH NPVGKSWRWKMD
Sbjct: 318  CRWTKSQLRGRGGCYKHAFYGIESHRCMEATPSLACANKCVFCWRHHANPVGKSWRWKMD 377

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            DPL IV+AAIDQHTKMIKQMKGVPGV QERL EGLSPRHCALSLVGEPIMYPEINTLIDE
Sbjct: 378  DPLEIVDAAIDQHTKMIKQMKGVPGVKQERLDEGLSPRHCALSLVGEPIMYPEINTLIDE 437

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LH+R ISTFLVTNAQFPD+IK LKPITQLYVSVDA+TK+SLKAIDRPLFGDFWERF+DSL
Sbjct: 438  LHQRRISTFLVTNAQFPDRIKMLKPITQLYVSVDASTKESLKAIDRPLFGDFWERFLDSL 497

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            KAL DKEQRTVYRLTLVKGWNAE+IDAYA+L  +GKPDLVEIKGVTYCGS+ATSKLTMEN
Sbjct: 498  KALHDKEQRTVYRLTLVKGWNAEEIDAYANLLTMGKPDLVEIKGVTYCGSSATSKLTMEN 557

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWHSDV+ FSEALA+KSNG YEVACEHVHSCCVLLAKVDK+K++G+W+TWIDYERF EL
Sbjct: 558  VPWHSDVKAFSEALAVKSNGIYEVACEHVHSCCVLLAKVDKFKVDGKWYTWIDYERFQEL 617

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGA 93
            VASG PF++EDY A+TPSWAVYGAEEGGFDP QSR+KKERRHGA
Sbjct: 618  VASGTPFRTEDYTALTPSWAVYGAEEGGFDPGQSRYKKERRHGA 661


>ref|NP_001347248.1| uncharacterized protein LOC100273754 [Zea mays]
 gb|ONM09874.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Zea mays]
          Length = 642

 Score =  726 bits (1874), Expect = 0.0
 Identities = 340/409 (83%), Positives = 371/409 (90%), Gaps = 1/409 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GKAP+R+        +NG  E    NG  NG ++MVTP+IR SL+KQGYKIIGSHSGVKI
Sbjct: 242  GKAPARR--------KNGNLEGVLANGVHNGVKDMVTPIIRTSLEKQGYKIIGSHSGVKI 293

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD
Sbjct: 294  CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMD 353

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            DPL IVNAAIDQHTKM+KQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DE
Sbjct: 354  DPLDIVNAAIDQHTKMVKQMKGVPGVKPEKLDEGLSPRHCALSLVGEPIMYPEINALVDE 413

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSL
Sbjct: 414  LHRRHISTFLVTNAQFPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSL 473

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            K+L +K+QRTVYRLTLVKGWNAE+IDAYA L D+G+PD +EIKGVTYCGS+ATSKLTMEN
Sbjct: 474  KSLHEKDQRTVYRLTLVKGWNAEEIDAYAKLLDLGQPDFIEIKGVTYCGSSATSKLTMEN 533

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWHSDV++FSE LA KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFHEL
Sbjct: 534  VPWHSDVKEFSEVLASKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHEL 593

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIRA 78
            V SGKPF S DYMA+TPSWAVYGAEEGGFDPDQSRFKKERRHG AA+R+
Sbjct: 594  VTSGKPFNSRDYMAMTPSWAVYGAEEGGFDPDQSRFKKERRHGTAALRS 642


>gb|KQK86877.1| hypothetical protein SETIT_034607mg [Setaria italica]
          Length = 649

 Score =  725 bits (1872), Expect = 0.0
 Identities = 339/408 (83%), Positives = 372/408 (91%), Gaps = 1/408 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GKAP+R         +NG  E S  NG +NG +EMVTP+IR SL+KQGYKI+GSHSGVKI
Sbjct: 250  GKAPAR---------QNGKVEGSLANGGQNGVKEMVTPIIRTSLEKQGYKILGSHSGVKI 300

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD
Sbjct: 301  CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMD 360

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            +PL I NAAIDQHTKMIKQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DE
Sbjct: 361  NPLDIANAAIDQHTKMIKQMKGVPGVKPEKLEEGLSPRHCALSLVGEPIMYPEINALVDE 420

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSL
Sbjct: 421  LHRRHISTFLVTNAQFPEKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSL 480

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            K+L +K+QRTVYRLTLVKGWNAE+IDAYA L ++G+PD +EIKGVTYCGS+ATSKLTMEN
Sbjct: 481  KSLHEKDQRTVYRLTLVKGWNAEEIDAYAKLLNLGQPDFIEIKGVTYCGSSATSKLTMEN 540

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWHSDV++FSE LA KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFHEL
Sbjct: 541  VPWHSDVKEFSEVLASKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHEL 600

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 81
            V SGKPFKS+DYMA+TPSWAVYGAEEGGFDPDQSRFKKERRHG AA++
Sbjct: 601  VTSGKPFKSQDYMALTPSWAVYGAEEGGFDPDQSRFKKERRHGTAALK 648


>ref|NP_001347259.1| uncharacterized LOC111828523 [Sorghum bicolor]
          Length = 652

 Score =  725 bits (1872), Expect = 0.0
 Identities = 340/406 (83%), Positives = 370/406 (91%), Gaps = 1/406 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GKAP+R+        +NG  E    NG +NG ++MVTP+IR SL+KQGYKIIGSHSGVKI
Sbjct: 250  GKAPARR--------KNGNLEGVVANGVQNGVKDMVTPIIRTSLEKQGYKIIGSHSGVKI 301

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD
Sbjct: 302  CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMD 361

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            DPL IVNAAIDQHTKM+KQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DE
Sbjct: 362  DPLDIVNAAIDQHTKMVKQMKGVPGVKPEKLEEGLSPRHCALSLVGEPIMYPEINALVDE 421

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSL
Sbjct: 422  LHRRHISTFLVTNAQFPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSL 481

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            K+L +K+QRTVYRLTLVKGWNAE+IDAYA L D+G+PD +EIKGVTYCGS+ATSKLTMEN
Sbjct: 482  KSLHEKDQRTVYRLTLVKGWNAEEIDAYAKLLDLGQPDFIEIKGVTYCGSSATSKLTMEN 541

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWHSDV++FSE LA KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFHEL
Sbjct: 542  VPWHSDVKEFSEVLASKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHEL 601

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAA 87
            V SGKPFKS DYMA+TPSWAVYGAEEGGFDPDQSRFKKERRHG AA
Sbjct: 602  VTSGKPFKSRDYMAMTPSWAVYGAEEGGFDPDQSRFKKERRHGTAA 647


>ref|NP_001347566.1| uncharacterized protein LOC111946219 [Setaria italica]
          Length = 692

 Score =  725 bits (1872), Expect = 0.0
 Identities = 339/408 (83%), Positives = 372/408 (91%), Gaps = 1/408 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GKAP+R         +NG  E S  NG +NG +EMVTP+IR SL+KQGYKI+GSHSGVKI
Sbjct: 293  GKAPAR---------QNGKVEGSLANGGQNGVKEMVTPIIRTSLEKQGYKILGSHSGVKI 343

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD
Sbjct: 344  CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMD 403

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            +PL I NAAIDQHTKMIKQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DE
Sbjct: 404  NPLDIANAAIDQHTKMIKQMKGVPGVKPEKLEEGLSPRHCALSLVGEPIMYPEINALVDE 463

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSL
Sbjct: 464  LHRRHISTFLVTNAQFPEKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSL 523

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            K+L +K+QRTVYRLTLVKGWNAE+IDAYA L ++G+PD +EIKGVTYCGS+ATSKLTMEN
Sbjct: 524  KSLHEKDQRTVYRLTLVKGWNAEEIDAYAKLLNLGQPDFIEIKGVTYCGSSATSKLTMEN 583

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWHSDV++FSE LA KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFHEL
Sbjct: 584  VPWHSDVKEFSEVLASKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHEL 643

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 81
            V SGKPFKS+DYMA+TPSWAVYGAEEGGFDPDQSRFKKERRHG AA++
Sbjct: 644  VTSGKPFKSQDYMALTPSWAVYGAEEGGFDPDQSRFKKERRHGTAALK 691


>ref|XP_020180383.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Aegilops tauschii subsp. tauschii]
          Length = 659

 Score =  721 bits (1860), Expect = 0.0
 Identities = 336/408 (82%), Positives = 374/408 (91%), Gaps = 1/408 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GKA ++K        +NG  E +  NG +NGAR MVTP+IRASL+KQGYKI+GSHSGVKI
Sbjct: 259  GKATAKK--------QNGKVESALPNGGQNGARAMVTPIIRASLEKQGYKIVGSHSGVKI 310

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD
Sbjct: 311  CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMD 370

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            DPL IVN AID+H KM+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYPEINTL+D+
Sbjct: 371  DPLDIVNTAIDEHRKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINTLVDD 430

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSL
Sbjct: 431  LHRRHISTFLVTNAQFPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSL 490

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            K+L DK+QRTVYRLTLVKGWN E+IDAYA+L  +G+PD +EIKGVTYCGS+ATSKLTMEN
Sbjct: 491  KSLHDKDQRTVYRLTLVKGWNVEEIDAYANLLKLGQPDFIEIKGVTYCGSSATSKLTMEN 550

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWH+DV++FSE LA KS G YE+ACEHVHSCCVLLAKVDK+KING+WHTWIDY+RF+EL
Sbjct: 551  VPWHADVKEFSEVLASKSGGVYELACEHVHSCCVLLAKVDKFKINGKWHTWIDYDRFNEL 610

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 81
            V SGKPFKS DYMAVTPSWAVYGA+EGGFDPDQ+RFKKERRHGAAA++
Sbjct: 611  VTSGKPFKSSDYMAVTPSWAVYGADEGGFDPDQARFKKERRHGAAALK 658


>dbj|BAK06688.1| predicted protein [Hordeum vulgare subsp. vulgare]
          Length = 664

 Score =  719 bits (1857), Expect = 0.0
 Identities = 335/408 (82%), Positives = 374/408 (91%), Gaps = 1/408 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GKA ++K        +NG  E +  NG +NGAR MVTP+IRASL+KQGYKI+GSHSGVKI
Sbjct: 264  GKATAKK--------QNGKVEGALPNGGQNGARAMVTPIIRASLEKQGYKIVGSHSGVKI 315

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD
Sbjct: 316  CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMD 375

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            DPL IVN AID+H KM+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYPEINTL+D+
Sbjct: 376  DPLDIVNTAIDEHRKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINTLVDD 435

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LHRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSL
Sbjct: 436  LHRRHISTFLVTNAQFPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSL 495

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            K+L DK+QRTVYRLTLVKGWN E+IDAYA+L  +G+PD +E+KGVTYCGS+ATSKLTMEN
Sbjct: 496  KSLHDKDQRTVYRLTLVKGWNVEEIDAYANLLKLGQPDFIEVKGVTYCGSSATSKLTMEN 555

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWH+DV++FSE LA KS G YE+ACEHVHSCCVLLAKVDK+KING+WHTWIDY+RF+EL
Sbjct: 556  VPWHADVKEFSEVLATKSGGVYELACEHVHSCCVLLAKVDKFKINGKWHTWIDYDRFNEL 615

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 81
            V SGKPFKS DYMAVTPSWAVYGA+EGGFDPDQ+RFKKERRHGAAA++
Sbjct: 616  VMSGKPFKSSDYMAVTPSWAVYGADEGGFDPDQARFKKERRHGAAALK 663


>gb|KZV28868.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Dorcoceras hygrometricum]
          Length = 658

 Score =  718 bits (1854), Expect = 0.0
 Identities = 343/410 (83%), Positives = 371/410 (90%), Gaps = 3/410 (0%)
 Frame = -1

Query: 1301 GKAPSRK-LVASLVNGENGVKEKSFVNGE--NGAREMVTPVIRASLQKQGYKIIGSHSGV 1131
            GK PSRK ++ +  NG+        VNGE  NG +EMVTPVIRASLQKQGYKIIGSHSGV
Sbjct: 254  GKGPSRKSIMGTTANGK--------VNGEVVNGEKEMVTPVIRASLQKQGYKIIGSHSGV 305

Query: 1130 KICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWK 951
            K+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWK
Sbjct: 306  KLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWK 365

Query: 950  MDDPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLI 771
            MDDPLVIV+ AID HTKMI+QMKGVPGV  ERLSEGLSPRHCALSLVGEPIMYPEIN LI
Sbjct: 366  MDDPLVIVDTAIDLHTKMIRQMKGVPGVKAERLSEGLSPRHCALSLVGEPIMYPEINALI 425

Query: 770  DELHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVD 591
            DELHRR ISTFLVTNAQFP++IK LKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFV+
Sbjct: 426  DELHRRRISTFLVTNAQFPERIKTLKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVE 485

Query: 590  SLKALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTM 411
            SLKALR+K+QRTVYRLTLVKGWN EDIDAY+SLFD+G PD +EIKGVTYCGS+ATSKLTM
Sbjct: 486  SLKALREKQQRTVYRLTLVKGWNTEDIDAYSSLFDLGNPDFIEIKGVTYCGSSATSKLTM 545

Query: 410  ENVPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFH 231
            ENVPWHSDV++FSEALA KSNG YEVACEH HSCCVLL KV K+K+ G+W TWIDY++FH
Sbjct: 546  ENVPWHSDVKNFSEALAQKSNGAYEVACEHEHSCCVLLGKVSKFKVEGEWFTWIDYDKFH 605

Query: 230  ELVASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 81
            +LVASGKPF SEDYM+ TP WAVYGAEEGGFDPDQSRFKKERRH  +A +
Sbjct: 606  DLVASGKPFTSEDYMSPTPRWAVYGAEEGGFDPDQSRFKKERRHHKSAAK 655


>ref|XP_014752193.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Brachypodium distachyon]
          Length = 653

 Score =  717 bits (1852), Expect = 0.0
 Identities = 334/407 (82%), Positives = 370/407 (90%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGSHSGVKIC 1122
            GKA +R+    +    NG         ENGAREMVTP+IR SL+KQGYKI+GSHSGVKIC
Sbjct: 254  GKATARRQNGKVAGLPNGA--------ENGAREMVTPIIRTSLEKQGYKIVGSHSGVKIC 305

Query: 1121 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD 942
            RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMDD
Sbjct: 306  RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDD 365

Query: 941  PLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDEL 762
            PL IVN AID+H KM+KQMKGVPGV  E L+EGLSPRHCALSLVGEPIMYPEINTL+D+L
Sbjct: 366  PLDIVNTAIDEHRKMVKQMKGVPGVKPELLAEGLSPRHCALSLVGEPIMYPEINTLVDDL 425

Query: 761  HRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLK 582
            HRRHISTFLVTNAQFP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSLK
Sbjct: 426  HRRHISTFLVTNAQFPEKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLK 485

Query: 581  ALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMENV 402
            +L DK+QRTVYRLTLVKGWNAE+IDAYA+L ++G+PD +EIKGVTYCGS+ATSKLTMENV
Sbjct: 486  SLHDKDQRTVYRLTLVKGWNAEEIDAYANLLNLGQPDFIEIKGVTYCGSSATSKLTMENV 545

Query: 401  PWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHELV 222
            PWH+DV++FSE LA KS G YE+ACEHVHSCCVLLAKVDK+KING+WHTWIDY+RFHELV
Sbjct: 546  PWHADVKEFSEVLASKSGGVYELACEHVHSCCVLLAKVDKFKINGKWHTWIDYDRFHELV 605

Query: 221  ASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 81
             SGKPFKS DYMA+TPSWAVYGAEEGGFDPDQSR+KKERRHG AA++
Sbjct: 606  TSGKPFKSCDYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGTAALK 652


>ref|XP_017981378.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Theobroma cacao]
          Length = 637

 Score =  717 bits (1850), Expect = 0.0
 Identities = 339/402 (84%), Positives = 365/402 (90%), Gaps = 1/402 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNGE-NGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GK PSRK   ++             NG+ +G REMVTPVIRA+L+KQGYKIIGSHSGVKI
Sbjct: 242  GKGPSRKKSVNVAE----------TNGKMDGKREMVTPVIRANLEKQGYKIIGSHSGVKI 291

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD
Sbjct: 292  CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWKMD 351

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            DPL IVN AID HTKMIKQMKGVPGVTQERL+EGL+PRHCALSLVGEPIMYPEIN L+DE
Sbjct: 352  DPLEIVNTAIDLHTKMIKQMKGVPGVTQERLTEGLTPRHCALSLVGEPIMYPEINMLVDE 411

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LHRR ISTFLVTNAQFPDKIK LKPITQLYVSVDAATKDSLKAIDRPLFGDFWERF+DSL
Sbjct: 412  LHRRRISTFLVTNAQFPDKIKMLKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSL 471

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            KALRDK+QRTVYRLTLVKGWN ED DAY+ LF IGKPD VEIKGVTYCGS+ATSKLTMEN
Sbjct: 472  KALRDKQQRTVYRLTLVKGWNTEDADAYSKLFSIGKPDFVEIKGVTYCGSSATSKLTMEN 531

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWHSDV+ FSEALA+KS G+YEVACEH HSCCVLLAK DK+K+NGQW+TWIDYE+FH+L
Sbjct: 532  VPWHSDVKAFSEALALKSEGEYEVACEHAHSCCVLLAKTDKFKVNGQWYTWIDYEKFHDL 591

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRH 99
            VASG+PF S DYMA+TPSWAVYGAEEGGFDPDQSR++KER H
Sbjct: 592  VASGEPFDSADYMALTPSWAVYGAEEGGFDPDQSRYRKERHH 633


>gb|EOY14757.1| Flavodoxin family protein / radical SAM domain-containing protein
            isoform 1 [Theobroma cacao]
          Length = 637

 Score =  717 bits (1850), Expect = 0.0
 Identities = 339/402 (84%), Positives = 365/402 (90%), Gaps = 1/402 (0%)
 Frame = -1

Query: 1301 GKAPSRKLVASLVNGENGVKEKSFVNGE-NGAREMVTPVIRASLQKQGYKIIGSHSGVKI 1125
            GK PSRK   ++             NG+ +G REMVTPVIRA+L+KQGYKIIGSHSGVKI
Sbjct: 242  GKGPSRKKSVNVAE----------TNGKMDGKREMVTPVIRANLEKQGYKIIGSHSGVKI 291

Query: 1124 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD 945
            CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD
Sbjct: 292  CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWKMD 351

Query: 944  DPLVIVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDE 765
            DPL IVN AID HTKMIKQMKGVPGVTQERL+EGL+PRHCALSLVGEPIMYPEIN L+DE
Sbjct: 352  DPLEIVNTAIDLHTKMIKQMKGVPGVTQERLTEGLTPRHCALSLVGEPIMYPEINMLVDE 411

Query: 764  LHRRHISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSL 585
            LHRR ISTFLVTNAQFPDKIK LKPITQLYVSVDAATKDSLKAIDRPLFGDFWERF+DSL
Sbjct: 412  LHRRRISTFLVTNAQFPDKIKMLKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSL 471

Query: 584  KALRDKEQRTVYRLTLVKGWNAEDIDAYASLFDIGKPDLVEIKGVTYCGSTATSKLTMEN 405
            KALRDK+QRTVYRLTLVKGWN ED DAY+ LF IGKPD VEIKGVTYCGS+ATSKLTMEN
Sbjct: 472  KALRDKQQRTVYRLTLVKGWNTEDADAYSKLFSIGKPDFVEIKGVTYCGSSATSKLTMEN 531

Query: 404  VPWHSDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHEL 225
            VPWHSDV+ FSEALA+KS G+YEVACEH HSCCVLLAK DK+K+NGQW+TWIDYE+FH+L
Sbjct: 532  VPWHSDVKAFSEALALKSEGEYEVACEHAHSCCVLLAKTDKFKVNGQWYTWIDYEKFHDL 591

Query: 224  VASGKPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRH 99
            VASG+PF S DYMA+TPSWAVYGAEEGGFDPDQSR++KER H
Sbjct: 592  VASGEPFDSADYMALTPSWAVYGAEEGGFDPDQSRYRKERHH 633