BLASTX nr result
ID: Ophiopogon24_contig00009790
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon24_contig00009790
(525 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAW82332.1| mannose/sialic acid-binding lectin [Polygonatum r... 94 4e-21
gb|AAC49413.1| mannose-specific lectin precursor [Polygonatum mu... 88 1e-18
pdb|3A0C|A Chain A, Crystal Structure Of An Anti-Hiv Mannose-Bin... 84 8e-18
gb|AAM77364.1|AF521656_1 mannose/sialic acid-binding lectin, par... 84 2e-17
ref|XP_020274747.1| mannose-specific lectin-like [Asparagus offi... 78 7e-15
gb|AAC49386.1| mannose-binding lectin precursor, partial [Tulipa... 75 8e-14
pdb|2D04|A Chain A, Crystal Structure Of Neoculin, A Sweet Prote... 72 5e-13
ref|XP_020101866.1| mannose-specific lectin-like [Ananas comosus] 73 6e-13
gb|PKU68030.1| Mannose-specific lectin [Dendrobium catenatum] 73 8e-13
gb|AAA19911.1| lectin, partial [Clivia miniata] 72 1e-12
sp|Q6F495.1|CURC2_MOLLA RecName: Full=Curculin-2; AltName: Full=... 72 2e-12
dbj|BAE45253.1| neoculin acid subunit [Molineria latifolia] 72 2e-12
gb|AAM27447.1|AF503622_1 lectin [Zephyranthes candida] >gi|21320... 70 7e-12
ref|XP_020683767.1| uncharacterized protein LOC110100548 [Dendro... 73 8e-12
gb|AAC49387.1| mannose-binding lectin precursor, partial [Tulipa... 70 1e-11
gb|AAA19912.1| lectin, partial [Clivia miniata] 69 1e-11
gb|AAA19578.1| lectin [Cymbidium hybrid cultivar] 68 6e-11
gb|AAA33345.1| lectin, partial [Galanthus nivalis] 67 7e-11
ref|XP_020104167.1| mannose-specific lectin-like [Ananas comosus] 67 7e-11
sp|B2KNH9.1|LEC_DENFN RecName: Full=Mannose-specific lectin; Alt... 67 8e-11
>gb|AAW82332.1| mannose/sialic acid-binding lectin [Polygonatum roseum]
Length = 159
Score = 94.0 bits (232), Expect = 4e-21
Identities = 49/115 (42%), Positives = 72/115 (62%), Gaps = 3/115 (2%)
Frame = -2
Query: 524 TSPNSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSL 345
TSPNSL SL +D Y L++ G+C+ V++D+G+P+ G LG C LTL N+G+L
Sbjct: 33 TSPNSLPSGHSLNSDSYRLIMQGDCNFVLYDSGSPVWASNTGG--LGSDCRLTLHNNGNL 90
Query: 344 VMYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGPSAFYPN---MLGATAGPH 189
V+YD+ N V+ +T+ G + +YVLVL D N V+YGP+ + +G T PH
Sbjct: 91 VIYDRGNNVIWETKTNGKDDHYVLVLQQDRNVVIYGPAVWATGSGPAVGLTLIPH 145
>gb|AAC49413.1| mannose-specific lectin precursor [Polygonatum multiflorum]
Length = 160
Score = 87.8 bits (216), Expect = 1e-18
Identities = 48/115 (41%), Positives = 66/115 (57%), Gaps = 3/115 (2%)
Frame = -2
Query: 524 TSPNSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSL 345
TSPNSL SL T Y L++ +C+LVV+D+G P+ G L C LT+ N+G+L
Sbjct: 34 TSPNSLPSGHSLNTGSYRLIMQADCNLVVYDSGKPVWATNTGG--LARDCRLTMHNNGNL 91
Query: 344 VMYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGPSAFYPN---MLGATAGPH 189
V+YD+ N V+ QT +YVLVL D N V+YGP+ + +G T PH
Sbjct: 92 VIYDRSNSVIWQTNTNEKEDHYVLVLQQDRNVVIYGPAVWATGSGPAVGLTLVPH 146
>pdb|3A0C|A Chain A, Crystal Structure Of An Anti-Hiv Mannose-Binding Lectin
From Polygonatum Cyrtonema Hua
pdb|3A0C|B Chain B, Crystal Structure Of An Anti-Hiv Mannose-Binding Lectin
From Polygonatum Cyrtonema Hua
pdb|3A0C|C Chain C, Crystal Structure Of An Anti-Hiv Mannose-Binding Lectin
From Polygonatum Cyrtonema Hua
pdb|3A0C|D Chain D, Crystal Structure Of An Anti-Hiv Mannose-Binding Lectin
From Polygonatum Cyrtonema Hua
pdb|3A0D|A Chain A, Crystal Structure Of Polygonatum Cyrtonema Lectin (Pcl)
Complexed With Monomannoside
pdb|3A0E|A Chain A, Crystal Structure Of Polygonatum Cyrtonema Lectin (pcl)
Complexed With Dimannoside
Length = 110
Score = 84.0 bits (206), Expect = 8e-18
Identities = 45/98 (45%), Positives = 61/98 (62%), Gaps = 1/98 (1%)
Frame = -2
Query: 524 TSPNSLHGRQSLTTD-KYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGS 348
+SPNSL SL Y L++ G+C+ V++D+G P+ G LG C LTL N+G+
Sbjct: 5 SSPNSLFTGHSLEVGPSYRLIMQGDCNFVLYDSGKPVWASNTGG--LGSGCRLTLHNNGN 62
Query: 347 LVMYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGP 234
LV+YDQ N V+ QT+ G +YVLVL D N V+YGP
Sbjct: 63 LVIYDQSNRVIWQTKTNGKEDHYVLVLQQDRNVVIYGP 100
>gb|AAM77364.1|AF521656_1 mannose/sialic acid-binding lectin, partial [Polygonatum cyrtonema]
gb|AAM28644.1| mannose/sialic acid-binding lectin precursor [Polygonatum
cyrtonema]
Length = 160
Score = 84.3 bits (207), Expect = 2e-17
Identities = 49/116 (42%), Positives = 67/116 (57%), Gaps = 4/116 (3%)
Frame = -2
Query: 524 TSPNSLHGRQSLTTD-KYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGS 348
+SPNSL SL Y L++ G+C+ V++D+G P+ G LG C LTL N+G+
Sbjct: 33 SSPNSLFTGHSLEVGPSYRLIMPGDCNFVLYDSGKPVWASNTGG--LGSGCRLTLHNNGN 90
Query: 347 LVMYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGPSAFYPN---MLGATAGPH 189
LV+YDQ N V+ QT+ G +YVLVL D N V+YGP + +G T PH
Sbjct: 91 LVIYDQSNRVIWQTKTNGKEDHYVLVLQQDRNVVIYGPVVWATGSGPAVGLTLIPH 146
>ref|XP_020274747.1| mannose-specific lectin-like [Asparagus officinalis]
Length = 160
Score = 77.8 bits (190), Expect = 7e-15
Identities = 40/97 (41%), Positives = 57/97 (58%)
Frame = -2
Query: 521 SPNSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLV 342
SP +L+ +SL Y + +C+LV++D+GNPI G G C ++ DG+LV
Sbjct: 31 SPEALYSGRSLNYGPYMFTMQNDCNLVLYDSGNPIWASNTGGR--GYNCRCVMQRDGNLV 88
Query: 341 MYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGPS 231
+YDQ N V + G +GNYVLVL D N V+YGP+
Sbjct: 89 VYDQNNNAVWASGTSGGDGNYVLVLQKDRNVVIYGPA 125
>gb|AAC49386.1| mannose-binding lectin precursor, partial [Tulipa hybrid cultivar]
prf||2207209C mannose-binding lectin:ISOTYPE=MII1
Length = 177
Score = 75.5 bits (184), Expect = 8e-14
Identities = 38/99 (38%), Positives = 60/99 (60%)
Frame = -2
Query: 512 SLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLVMYD 333
SL+G QSLT + Y+ ++ +C+LV+++ PI + LG C++T++ DG+LV+Y
Sbjct: 52 SLYGGQSLTWESYTFIMQTDCNLVLYEGNGPIWA--SGSNDLGSGCYVTMQKDGNLVIYS 109
Query: 332 QYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGPSAFYPN 216
+ V ++ GNYVLVL D N V+YGPS + N
Sbjct: 110 KSGNSVWASQTHQAEGNYVLVLQKDRNVVIYGPSLWATN 148
>pdb|2D04|A Chain A, Crystal Structure Of Neoculin, A Sweet Protein With
Taste-Modifying Activity.
pdb|2D04|C Chain C, Crystal Structure Of Neoculin, A Sweet Protein With
Taste-Modifying Activity.
pdb|2D04|E Chain E, Crystal Structure Of Neoculin, A Sweet Protein With
Taste-Modifying Activity.
pdb|2D04|G Chain G, Crystal Structure Of Neoculin, A Sweet Protein With
Taste-Modifying Activity.
Length = 113
Score = 71.6 bits (174), Expect = 5e-13
Identities = 40/96 (41%), Positives = 54/96 (56%)
Frame = -2
Query: 521 SPNSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLV 342
S +L+ SLT+ Y+L + NC+LV + G I G G C LTL +DG+L+
Sbjct: 6 SGQTLYAGHSLTSGSYTLTIQNNCNLVKYQHGRQIWASDTDGQ--GSQCRLTLRSDGNLI 63
Query: 341 MYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGP 234
+YD N VV ++ G NG Y LVL DG V+YGP
Sbjct: 64 IYDDNNMVVWGSDCWGNNGTYALVLQQDGLFVIYGP 99
>ref|XP_020101866.1| mannose-specific lectin-like [Ananas comosus]
Length = 164
Score = 72.8 bits (177), Expect = 6e-13
Identities = 37/96 (38%), Positives = 59/96 (61%), Gaps = 1/96 (1%)
Frame = -2
Query: 515 NSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTH-LGPICHLTLENDGSLVM 339
++L+G QSLT Y L + +C+LV++D GN + + GT+ G C+ +++DG+LV+
Sbjct: 31 DALYGGQSLTWGNYRLTMQTDCNLVLYDGGNAVW---SSGTYNQGSGCYAKMQSDGNLVV 87
Query: 338 YDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGPS 231
Y YN + T + GNY+ VL D N V+YGP+
Sbjct: 88 YGGYNNALWATNTEREQGNYICVLQRDRNVVIYGPA 123
>gb|PKU68030.1| Mannose-specific lectin [Dendrobium catenatum]
Length = 173
Score = 72.8 bits (177), Expect = 8e-13
Identities = 34/86 (39%), Positives = 56/86 (65%)
Frame = -2
Query: 497 QSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLVMYDQYNEV 318
Q+LT ++ V+ +C+LV+++ GNP+ G LG C++TL++DG+LV+YDQ N+
Sbjct: 40 QALTQGGFAFVIQSDCNLVLYEFGNPLWSSGTNGQGLG--CYVTLQSDGNLVIYDQSNKA 97
Query: 317 VCQTEAKGPNGNYVLVLLPDGNPVVY 240
+ + G GNY+L+L D N V+Y
Sbjct: 98 IWASNTNGETGNYLLILQKDRNVVIY 123
>gb|AAA19911.1| lectin, partial [Clivia miniata]
Length = 169
Score = 72.0 bits (175), Expect = 1e-12
Identities = 36/99 (36%), Positives = 57/99 (57%)
Frame = -2
Query: 521 SPNSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLV 342
S +L +SLT +Y ++ +C+LV++D PI G G CHL+++ DG+LV
Sbjct: 36 SGETLSPGESLTYGRYDFIIQEDCNLVLYDVDKPIWATNTGGVSSG--CHLSMQTDGNLV 93
Query: 341 MYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGPSAF 225
+Y N + + +G NGNYV VL D N V+YG +++
Sbjct: 94 VYSPKNNPIWASNTEGENGNYVCVLQKDRNVVIYGTASW 132
>sp|Q6F495.1|CURC2_MOLLA RecName: Full=Curculin-2; AltName: Full=Neoculin acidic subunit;
Short=NAS; Flags: Precursor
dbj|BAD29946.1| neoculin acidic subunit [Molineria latifolia]
dbj|BAD38841.1| curculin [Molineria latifolia]
Length = 158
Score = 71.6 bits (174), Expect = 2e-12
Identities = 40/96 (41%), Positives = 54/96 (56%)
Frame = -2
Query: 521 SPNSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLV 342
S +L+ SLT+ Y+L + NC+LV + G I G G C LTL +DG+L+
Sbjct: 28 SGQTLYAGHSLTSGSYTLTIQNNCNLVKYQHGRQIWASDTDGQ--GSQCRLTLRSDGNLI 85
Query: 341 MYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGP 234
+YD N VV ++ G NG Y LVL DG V+YGP
Sbjct: 86 IYDDNNMVVWGSDCWGNNGTYALVLQQDGLFVIYGP 121
>dbj|BAE45253.1| neoculin acid subunit [Molineria latifolia]
Length = 168
Score = 71.6 bits (174), Expect = 2e-12
Identities = 40/96 (41%), Positives = 54/96 (56%)
Frame = -2
Query: 521 SPNSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLV 342
S +L+ SLT+ Y+L + NC+LV + G I G G C LTL +DG+L+
Sbjct: 38 SGQTLYAGHSLTSGSYTLTIQNNCNLVKYQHGRQIWASDTDGQ--GSQCRLTLRSDGNLI 95
Query: 341 MYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGP 234
+YD N VV ++ G NG Y LVL DG V+YGP
Sbjct: 96 IYDDNNMVVWGSDCWGNNGTYALVLQQDGLFVIYGP 131
>gb|AAM27447.1|AF503622_1 lectin [Zephyranthes candida]
gb|AAM44412.1| agglutinin [Zephyranthes candida]
Length = 169
Score = 70.1 bits (170), Expect = 7e-12
Identities = 37/98 (37%), Positives = 56/98 (57%), Gaps = 1/98 (1%)
Frame = -2
Query: 521 SPNSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLV 342
S +L QSL Y+ ++ +C+LV++D PI G G CHL+++ DG+LV
Sbjct: 31 SGETLSAGQSLNNGPYTFIMQEDCNLVLYDVDKPIWASNTGGLARG--CHLSMQRDGNLV 88
Query: 341 MYDQYNEVVCQTEAKGPN-GNYVLVLLPDGNPVVYGPS 231
+Y Q N + + +G N NYVL+L D N V+YGP+
Sbjct: 89 VYSQRNRPIWASNTRGHNAANYVLILQKDRNVVIYGPA 126
>ref|XP_020683767.1| uncharacterized protein LOC110100548 [Dendrobium catenatum]
Length = 399
Score = 72.8 bits (177), Expect = 8e-12
Identities = 34/86 (39%), Positives = 56/86 (65%)
Frame = -2
Query: 497 QSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLVMYDQYNEV 318
Q+LT ++ V+ +C+LV+++ GNP+ G LG C++TL++DG+LV+YDQ N+
Sbjct: 40 QALTQGGFAFVIQSDCNLVLYEFGNPLWSSGTNGQGLG--CYVTLQSDGNLVIYDQSNKA 97
Query: 317 VCQTEAKGPNGNYVLVLLPDGNPVVY 240
+ + G GNY+L+L D N V+Y
Sbjct: 98 IWASNTNGETGNYLLILQKDRNVVIY 123
Score = 62.0 bits (149), Expect = 5e-08
Identities = 30/90 (33%), Positives = 54/90 (60%)
Frame = -2
Query: 509 LHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLVMYDQ 330
L+ Q+LT YS ++ +C+LV+++ G+ I G G C++T++ DG+LV+Y++
Sbjct: 262 LNTGQALTQGGYSFIIQSDCNLVLYEYGSAIWSSGTNGKGKG--CYVTMQTDGNLVIYNR 319
Query: 329 YNEVVCQTEAKGPNGNYVLVLLPDGNPVVY 240
N+ + + GNY+L+L D N V+Y
Sbjct: 320 KNKAIWASNTNRETGNYILILQKDRNVVIY 349
>gb|AAC49387.1| mannose-binding lectin precursor, partial [Tulipa hybrid cultivar]
prf||2207209D mannose-binding lectin:ISOTYPE=MII2
Length = 183
Score = 70.1 bits (170), Expect = 1e-11
Identities = 37/99 (37%), Positives = 59/99 (59%)
Frame = -2
Query: 512 SLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLVMYD 333
SL+G QSLT + Y+ ++ +C+LV+++ I + G LG C++T++ DG+LV+Y
Sbjct: 58 SLYGGQSLTWESYTFIMQTDCNLVLYEGNGSIWASGSNG--LGSGCYVTMQKDGNLVIYS 115
Query: 332 QYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGPSAFYPN 216
+ V ++ GNYVLVL D N V+YG S + N
Sbjct: 116 KIGNSVWASQTHQAEGNYVLVLQKDRNVVIYGSSLWATN 154
>gb|AAA19912.1| lectin, partial [Clivia miniata]
Length = 167
Score = 69.3 bits (168), Expect = 1e-11
Identities = 36/95 (37%), Positives = 54/95 (56%)
Frame = -2
Query: 521 SPNSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLV 342
S +L + L+ +Y ++ +C+LV++D PI G G CHL+L+ DG+LV
Sbjct: 34 SGETLSAGEYLSYGRYVFIMQEDCNLVLYDVDKPIWATNTGGISSG--CHLSLQTDGNLV 91
Query: 341 MYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYG 237
+Y Q N + ++ G NGNYV VL D N V+YG
Sbjct: 92 VYRQRNNPIWESNTGGENGNYVCVLQKDRNVVIYG 126
>gb|AAA19578.1| lectin [Cymbidium hybrid cultivar]
Length = 176
Score = 67.8 bits (164), Expect = 6e-11
Identities = 37/95 (38%), Positives = 57/95 (60%), Gaps = 1/95 (1%)
Frame = -2
Query: 521 SPNSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTH-LGPICHLTLENDGSL 345
S L+ QSLT+ L + +C+LV++D G + I + GT+ G C++TL+ DG+L
Sbjct: 35 SGERLNPGQSLTSGNVDLAMQYDCNLVLYDNG---KSIWSSGTYGSGSGCYVTLQTDGNL 91
Query: 344 VMYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVY 240
V+YD N + + G NGNY+L+L D N V+Y
Sbjct: 92 VIYDNTNNPLWASNTSGENGNYILILQKDRNLVIY 126
>gb|AAA33345.1| lectin, partial [Galanthus nivalis]
Length = 161
Score = 67.4 bits (163), Expect = 7e-11
Identities = 33/97 (34%), Positives = 54/97 (55%)
Frame = -2
Query: 521 SPNSLHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLV 342
S +L + L Y ++ +C+LV++D P+ E G L C+L ++ DG+LV
Sbjct: 28 SGETLSAGEFLNQGNYVFIMQEDCNLVLYDVDKPLWETNTGG--LSRRCYLNMQTDGNLV 85
Query: 341 MYDQYNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGPS 231
+Y+ N+ + + G NGNYV +L DGN +YGP+
Sbjct: 86 VYNPSNKPIWASNTGGQNGNYVCILQKDGNIAIYGPA 122
>ref|XP_020104167.1| mannose-specific lectin-like [Ananas comosus]
Length = 166
Score = 67.4 bits (163), Expect = 7e-11
Identities = 35/93 (37%), Positives = 53/93 (56%)
Frame = -2
Query: 509 LHGRQSLTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLVMYDQ 330
L G +SLT Y + +C+LV++D G+P+ + H G C L +++DG+LV+YD
Sbjct: 34 LSGGESLTYGAYRFTMQTDCNLVLYDGGSPVWA--SQTYHKGTGCALKMQSDGNLVIYDS 91
Query: 329 YNEVVCQTEAKGPNGNYVLVLLPDGNPVVYGPS 231
+ + G GNYV +L D N VVYGP+
Sbjct: 92 SGSPLWASNTGGEQGNYVCILQRDRNVVVYGPA 124
>sp|B2KNH9.1|LEC_DENFN RecName: Full=Mannose-specific lectin; AltName: Full=Agglutinin;
Flags: Precursor
gb|ABU62812.1| agglutinin [Dendrobium findlayanum]
Length = 167
Score = 67.4 bits (163), Expect = 8e-11
Identities = 37/105 (35%), Positives = 60/105 (57%), Gaps = 7/105 (6%)
Frame = -2
Query: 491 LTTDKYSLVLSGNCSLVVHDAGNPIREIINCGTHLGPICHLTLENDGSLVMYDQY----- 327
L D+Y L++ +C+LV+++ P E + G C +TL++DG+ V+YD++
Sbjct: 40 LKQDRYMLIMQEDCNLVLYNLNKP--EWATKTANQGSRCFVTLQSDGNFVIYDEHEQEGR 97
Query: 326 NEVVCQTEAKGPNGNYVLVLLPDGNPVVYGPSAFY--PNMLGATA 198
NE + ++ G NGNYV++L DGN V+Y F N G+TA
Sbjct: 98 NEAIWASKTDGENGNYVIILQKDGNLVLYSKPIFATGTNRFGSTA 142