BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon24_contig00009288
         (791 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ONK71489.1| uncharacterized protein A4U43_C04F9180 [Asparagus...   110   2e-23
ref|XP_020262391.1| LOW QUALITY PROTEIN: DNA topoisomerase 2-lik...   110   2e-23
ref|XP_020261630.1| SKP1-like protein 1A [Asparagus officinalis]...   101   5e-23
ref|XP_008779357.2| PREDICTED: DNA topoisomerase 2 [Phoenix dact...   103   3e-21
gb|PKA59619.1| SKP1-like protein 1B [Apostasia shenzhenica]            96   8e-21
ref|XP_020703085.1| DNA topoisomerase 2-like [Dendrobium catenat...   101   1e-20
gb|AGF33287.1| SKP1 1A-like protein, partial [Quercus ilex]            94   2e-20
ref|XP_020693654.1| DNA topoisomerase 2 [Dendrobium catenatum] >...   100   3e-20
ref|XP_023893685.1| SKP1-like protein 1B isoform X1 [Quercus sub...    94   5e-20
gb|OVA09460.1| DNA topoisomerase II [Macleaya cordata]                100   5e-20
ref|XP_020276051.1| SKP1-like protein 1B [Asparagus officinalis]...    94   5e-20
gb|POO00815.1| S-phase kinase-associated protein [Trema orientalis]    93   1e-19
gb|PON53488.1| S-phase kinase-associated protein [Parasponia and...    93   1e-19
ref|XP_021668106.1| SKP1-like protein 1B [Hevea brasiliensis]          93   1e-19
gb|PKA63063.1| DNA topoisomerase 2 [Apostasia shenzhenica]             99   1e-19
gb|OVA19386.1| DNA topoisomerase II [Macleaya cordata]                 99   1e-19
ref|XP_021908138.1| SKP1-like protein 1B [Carica papaya]               92   1e-19
ref|XP_021600553.1| SKP1-like protein 1B [Manihot esculenta] >gi...    92   1e-19
ref|XP_010920068.1| PREDICTED: DNA topoisomerase 2 [Elaeis guine...    99   2e-19
ref|XP_020113224.1| DNA topoisomerase 2-like [Ananas comosus]          98   2e-19

>gb|ONK71489.1| uncharacterized protein A4U43_C04F9180 [Asparagus officinalis]
          Length = 1419

 Score =  110 bits (274), Expect = 2e-23
 Identities = 63/124 (50%), Positives = 82/124 (66%), Gaps = 18/124 (14%)
 Frame = -1

Query: 614  EVLLEKMEYELLKFDNKVRFILSIVEGKITFSQRSE-DWFL----KGFTPFPTKTKDIDA 450
            ++LL+ +E++LLK DNKVRFIL +V G+I  + R   D FL    KGFTPFP KTK IDA
Sbjct: 958  KILLDNLEFDLLKLDNKVRFILGVVRGEIIVNNRKRVDLFLELHQKGFTPFPKKTKGIDA 1017

Query: 449  AIEGATVN-------------GVVRKSDYEYLLSMPIRNLFPEGFRELCADKQKLENKLK 309
            AI GATV              G V+ SDYEYLLS+ I  L  E  + LCADK++LE++L+
Sbjct: 1018 AIAGATVETEENEEESPEMVKGAVKASDYEYLLSLAIGTLTLEKVQALCADKERLEHELE 1077

Query: 308  VLKS 297
            +L+S
Sbjct: 1078 MLRS 1081


>ref|XP_020262391.1| LOW QUALITY PROTEIN: DNA topoisomerase 2-like [Asparagus officinalis]
          Length = 1489

 Score =  110 bits (274), Expect = 2e-23
 Identities = 63/124 (50%), Positives = 82/124 (66%), Gaps = 18/124 (14%)
 Frame = -1

Query: 614  EVLLEKMEYELLKFDNKVRFILSIVEGKITFSQRSE-DWFL----KGFTPFPTKTKDIDA 450
            ++LL+ +E++LLK DNKVRFIL +V G+I  + R   D FL    KGFTPFP KTK IDA
Sbjct: 1028 KILLDNLEFDLLKLDNKVRFILGVVRGEIIVNNRKRVDLFLELHQKGFTPFPKKTKGIDA 1087

Query: 449  AIEGATVN-------------GVVRKSDYEYLLSMPIRNLFPEGFRELCADKQKLENKLK 309
            AI GATV              G V+ SDYEYLLS+ I  L  E  + LCADK++LE++L+
Sbjct: 1088 AIAGATVETEENEEESPEMVKGAVKASDYEYLLSLAIGTLTLEKVQALCADKERLEHELE 1147

Query: 308  VLKS 297
            +L+S
Sbjct: 1148 MLRS 1151


>ref|XP_020261630.1| SKP1-like protein 1A [Asparagus officinalis]
 gb|ONK72621.1| uncharacterized protein A4U43_C04F21310 [Asparagus officinalis]
          Length = 161

 Score =  101 bits (252), Expect = 5e-23
 Identities = 56/103 (54%), Positives = 69/103 (66%)
 Frame = -1

Query: 788 IPLPNVNSKILSKVIEYSKKHVDSTSSKSDEKTADEELKAWDAEFVKVDQATLFDLILEV 609
           IPLPNVNSKILSKVIEY KKHVD+ S+K+D+K+ DEELKAWDA+FVKVDQ TLFDLIL  
Sbjct: 47  IPLPNVNSKILSKVIEYCKKHVDAASAKTDDKSGDEELKAWDADFVKVDQPTLFDLILAA 106

Query: 608 LLEKMEYELLKFDNKVRFILSIVEGKITFSQRSEDWFLKGFTP 480
               ++  L   D   + +  +++GK     R        FTP
Sbjct: 107 NYLNIKSLL---DLTCQTVADMIKGKTPEEIRKTFNIKNDFTP 146


>ref|XP_008779357.2| PREDICTED: DNA topoisomerase 2 [Phoenix dactylifera]
          Length = 1378

 Score =  103 bits (257), Expect = 3e-21
 Identities = 62/118 (52%), Positives = 76/118 (64%), Gaps = 13/118 (11%)
 Frame = -1

Query: 614  EVLLEKMEYELLKFDNKVRFILSIVEGKITFSQRSE-DWFL----KGFTPFPTKTKDIDA 450
            +VLL+ +E +LLK DNKVRFIL +V G+I  S R   D FL    KGFTPFP K+K IDA
Sbjct: 927  KVLLDNLEMDLLKLDNKVRFILGVVRGEIIVSNRKRADLFLELQQKGFTPFPKKSKGIDA 986

Query: 449  AIEGATVNG--------VVRKSDYEYLLSMPIRNLFPEGFRELCADKQKLENKLKVLK 300
            AI GAT             R +DYEYLLSM I  L  E  +ELCAD++KLE +++ LK
Sbjct: 987  AIAGATEEDDNEESPEVAARATDYEYLLSMAIGTLTWEKVQELCADRKKLEGEVEELK 1044


>gb|PKA59619.1| SKP1-like protein 1B [Apostasia shenzhenica]
          Length = 162

 Score = 95.9 bits (237), Expect = 8e-21
 Identities = 49/61 (80%), Positives = 53/61 (86%), Gaps = 3/61 (4%)
 Frame = -1

Query: 788 IPLPNVNSKILSKVIEYSKKHVDSTSSKS---DEKTADEELKAWDAEFVKVDQATLFDLI 618
           IPLPNVNSKIL+KVIEY KKHV+STS  S   D+K+ DEELK WDAEFVKVDQATLFDLI
Sbjct: 45  IPLPNVNSKILAKVIEYCKKHVESTSKSSAAGDDKSVDEELKNWDAEFVKVDQATLFDLI 104

Query: 617 L 615
           L
Sbjct: 105 L 105


>ref|XP_020703085.1| DNA topoisomerase 2-like [Dendrobium catenatum]
 gb|PKU64218.1| DNA topoisomerase 2 [Dendrobium catenatum]
          Length = 1268

 Score =  101 bits (252), Expect = 1e-20
 Identities = 60/129 (46%), Positives = 79/129 (61%), Gaps = 17/129 (13%)
 Frame = -1

Query: 635  TLFDLILEVLLEKMEYELLKFDNKVRFILSIVEGKITFSQRSE-DWFL----KGFTPFPT 471
            T ++   + LL+ +E +LLK DNKVRFIL +V+G+I  + R   + FL    KGFTPFP 
Sbjct: 823  TYYEKRKKALLDNLELDLLKLDNKVRFILGVVKGQIIVNNRKRAELFLELQEKGFTPFPK 882

Query: 470  KTKDIDAAIEGAT------------VNGVVRKSDYEYLLSMPIRNLFPEGFRELCADKQK 327
            KTK +D AI GAT              G V+ SDY+YLLSM I  L  E  +ELCA+K+K
Sbjct: 883  KTKGVDVAIAGATPETEESEETTEVAKGGVKPSDYDYLLSMAIGTLTWEKVQELCAEKEK 942

Query: 326  LENKLKVLK 300
             EN +K L+
Sbjct: 943  FENDVKELR 951


>gb|AGF33287.1| SKP1 1A-like protein, partial [Quercus ilex]
          Length = 124

 Score = 93.6 bits (231), Expect = 2e-20
 Identities = 54/103 (52%), Positives = 68/103 (66%)
 Frame = -1

Query: 788 IPLPNVNSKILSKVIEYSKKHVDSTSSKSDEKTADEELKAWDAEFVKVDQATLFDLILEV 609
           IPLPNV SKIL+KVIEY KKHV+STS+  DE+T+DE+LKAWD EFV+VDQATLFDLIL  
Sbjct: 27  IPLPNVTSKILAKVIEYCKKHVESTSA--DERTSDEDLKAWDTEFVRVDQATLFDLILAA 84

Query: 608 LLEKMEYELLKFDNKVRFILSIVEGKITFSQRSEDWFLKGFTP 480
               ++  L   D   + +  +++GK     R        FTP
Sbjct: 85  NYLNIKSLL---DLTCQTVADMIKGKTPEEIRKTFNIKNDFTP 124


>ref|XP_020693654.1| DNA topoisomerase 2 [Dendrobium catenatum]
 gb|PKU72193.1| DNA topoisomerase 2 [Dendrobium catenatum]
          Length = 1466

 Score =  100 bits (250), Expect = 3e-20
 Identities = 59/129 (45%), Positives = 80/129 (62%), Gaps = 17/129 (13%)
 Frame = -1

Query: 635  TLFDLILEVLLEKMEYELLKFDNKVRFILSIVEGKITFSQRSE-DWFL----KGFTPFPT 471
            T ++   + LL+ +E +LLK DNKVRFIL +V+G+I  + R   + FL    KGFTPFP 
Sbjct: 1021 TYYEKRKKALLDNLELDLLKLDNKVRFILGVVKGQIIVNNRKRAELFLELQEKGFTPFPK 1080

Query: 470  KTKDIDAAIEGAT------------VNGVVRKSDYEYLLSMPIRNLFPEGFRELCADKQK 327
            KTK +D AI GAT            V G V+ SDY+YLLS+ I  L  E  +ELC++K+K
Sbjct: 1081 KTKGVDVAIAGATPETEESEETTEVVKGGVKPSDYDYLLSLAIGTLTWEKVQELCSEKEK 1140

Query: 326  LENKLKVLK 300
             EN +K L+
Sbjct: 1141 FENDVKELR 1149


>ref|XP_023893685.1| SKP1-like protein 1B isoform X1 [Quercus suber]
 ref|XP_023893686.1| SKP1-like protein 1B isoform X2 [Quercus suber]
          Length = 155

 Score = 93.6 bits (231), Expect = 5e-20
 Identities = 54/103 (52%), Positives = 68/103 (66%)
 Frame = -1

Query: 788 IPLPNVNSKILSKVIEYSKKHVDSTSSKSDEKTADEELKAWDAEFVKVDQATLFDLILEV 609
           IPLPNV SKIL+KVIEY KKHV+STS+  DE+T+DE+LKAWD EFV+VDQATLFDLIL  
Sbjct: 43  IPLPNVTSKILAKVIEYCKKHVESTSA--DERTSDEDLKAWDTEFVRVDQATLFDLILAA 100

Query: 608 LLEKMEYELLKFDNKVRFILSIVEGKITFSQRSEDWFLKGFTP 480
               ++  L   D   + +  +++GK     R        FTP
Sbjct: 101 NYLNIKSLL---DLTCQTVADMIKGKTPEEIRKTFNIKNDFTP 140


>gb|OVA09460.1| DNA topoisomerase II [Macleaya cordata]
          Length = 631

 Score = 99.8 bits (247), Expect = 5e-20
 Identities = 63/130 (48%), Positives = 80/130 (61%), Gaps = 18/130 (13%)
 Frame = -1

Query: 632 LFDLILEVLLEKMEYELLKFDNKVRFILSIVEGKITFSQRSE-DWFL----KGFTPFPTK 468
           L++   +VLL+ +E ELLK DNKVRFIL +V G+I  S R   D FL    KGFTPFP K
Sbjct: 149 LYEKRKKVLLDNLELELLKLDNKVRFILGVVRGEIIVSNRKRVDLFLELQQKGFTPFPKK 208

Query: 467 TKDIDAAIEGAT------------VNG-VVRKSDYEYLLSMPIRNLFPEGFRELCADKQK 327
           TK+++A + GAT            VN   VR  DYEYLLSM I  L  E  +ELC+DK +
Sbjct: 209 TKNVEALVVGATEDIEENEESQEVVNSRGVRAIDYEYLLSMAIGTLTLEKVQELCSDKDR 268

Query: 326 LENKLKVLKS 297
           L N++  L+S
Sbjct: 269 LTNEVDELRS 278


>ref|XP_020276051.1| SKP1-like protein 1B [Asparagus officinalis]
 gb|ONK64492.1| uncharacterized protein A4U43_C07F26640 [Asparagus officinalis]
          Length = 156

 Score = 93.6 bits (231), Expect = 5e-20
 Identities = 48/58 (82%), Positives = 52/58 (89%)
 Frame = -1

Query: 788 IPLPNVNSKILSKVIEYSKKHVDSTSSKSDEKTADEELKAWDAEFVKVDQATLFDLIL 615
           IPLPNVNSKIL+KVIEY KKHV+  SS SDEKTA +E+KAWD EFVKVDQATLFDLIL
Sbjct: 43  IPLPNVNSKILAKVIEYCKKHVEKGSS-SDEKTAADEIKAWDEEFVKVDQATLFDLIL 99


>gb|POO00815.1| S-phase kinase-associated protein [Trema orientalis]
          Length = 156

 Score = 92.8 bits (229), Expect = 1e-19
 Identities = 54/103 (52%), Positives = 67/103 (65%)
 Frame = -1

Query: 788 IPLPNVNSKILSKVIEYSKKHVDSTSSKSDEKTADEELKAWDAEFVKVDQATLFDLILEV 609
           IPLPNV SKIL+KVIEY KKHVDST+   DE+ AD++LK+WDAEFVKVDQATLFDLIL  
Sbjct: 44  IPLPNVTSKILAKVIEYCKKHVDSTNP--DERVADDDLKSWDAEFVKVDQATLFDLILAA 101

Query: 608 LLEKMEYELLKFDNKVRFILSIVEGKITFSQRSEDWFLKGFTP 480
               ++  L   D   + +  +++GK     R        FTP
Sbjct: 102 NYLNIKNLL---DLTCQTVADMIKGKTPEEIRKTFNIKNDFTP 141


>gb|PON53488.1| S-phase kinase-associated protein [Parasponia andersonii]
          Length = 156

 Score = 92.8 bits (229), Expect = 1e-19
 Identities = 54/103 (52%), Positives = 67/103 (65%)
 Frame = -1

Query: 788 IPLPNVNSKILSKVIEYSKKHVDSTSSKSDEKTADEELKAWDAEFVKVDQATLFDLILEV 609
           IPLPNV SKIL+KVIEY KKHVDST+   DE+ AD++LK+WDAEFVKVDQATLFDLIL  
Sbjct: 44  IPLPNVTSKILAKVIEYCKKHVDSTNP--DERVADDDLKSWDAEFVKVDQATLFDLILAA 101

Query: 608 LLEKMEYELLKFDNKVRFILSIVEGKITFSQRSEDWFLKGFTP 480
               ++  L   D   + +  +++GK     R        FTP
Sbjct: 102 NYLNIKNLL---DLTCQTVADMIKGKTPEEIRKTFNIKNDFTP 141


>ref|XP_021668106.1| SKP1-like protein 1B [Hevea brasiliensis]
          Length = 157

 Score = 92.8 bits (229), Expect = 1e-19
 Identities = 52/103 (50%), Positives = 66/103 (64%)
 Frame = -1

Query: 788 IPLPNVNSKILSKVIEYSKKHVDSTSSKSDEKTADEELKAWDAEFVKVDQATLFDLILEV 609
           IPLPNV SKILSKVIEY KKHV+++ S     + D+ELKAWDAEFVKVDQATLFDLIL  
Sbjct: 43  IPLPNVTSKILSKVIEYCKKHVETSKSDDRPSSVDDELKAWDAEFVKVDQATLFDLILAA 102

Query: 608 LLEKMEYELLKFDNKVRFILSIVEGKITFSQRSEDWFLKGFTP 480
               ++  L   D+  + +  +++GK     R+       FTP
Sbjct: 103 NYLNIKSLL---DHTCQTVADMIKGKTPEEIRTTFNIRNDFTP 142


>gb|PKA63063.1| DNA topoisomerase 2 [Apostasia shenzhenica]
          Length = 1404

 Score = 99.0 bits (245), Expect = 1e-19
 Identities = 66/178 (37%), Positives = 98/178 (55%), Gaps = 20/178 (11%)
 Frame = -1

Query: 770  NSKILSKVIEYSKKHVDSTSSKSDEKTADEELKAWDAEFVKVD---QATLFDLILEVLLE 600
            N KI    I+  +++ D TS   +   ++E L     E +K     + T ++   + LL+
Sbjct: 941  NDKIKEPFIKDYREYNDDTSVHFEVTLSEENLNLAKQEVLKEFFYLRITFYEKRKKALLD 1000

Query: 599  KMEYELLKFDNKVRFILSIVEGKITFSQRSEDWFL-----KGFTPFPTKTKDIDAAIEGA 435
              E +LLK +NKVRFIL   +G+I  + R E+        KGFTPFP KTK I+AAI GA
Sbjct: 1001 SSENDLLKLENKVRFILESNKGEIILTNRDEEDLARELQEKGFTPFPKKTKGIEAAIAGA 1060

Query: 434  T------------VNGVVRKSDYEYLLSMPIRNLFPEGFRELCADKQKLENKLKVLKS 297
                         V G V+ SDYEYLLSM I  L  +   +LC++K+KLE++++ L++
Sbjct: 1061 IAEVEEAEEAIDLVEGGVKASDYEYLLSMAIYTLTTKKAEQLCSEKRKLEDQVQKLRN 1118


>gb|OVA19386.1| DNA topoisomerase II [Macleaya cordata]
          Length = 1515

 Score = 99.0 bits (245), Expect = 1e-19
 Identities = 61/122 (50%), Positives = 75/122 (61%), Gaps = 18/122 (14%)
 Frame = -1

Query: 611  VLLEKMEYELLKFDNKVRFILSIVEGKITFSQRSE-DWFL----KGFTPFPTKTKDIDAA 447
            VLLE +E ELLK DNKVRFIL +V+G+I  S R   D FL    KGFTPFP KTK ++A 
Sbjct: 1054 VLLENLELELLKLDNKVRFILGVVKGEIIVSNRKRADLFLELQQKGFTPFPKKTKLVEAV 1113

Query: 446  IEGATVNGV-------------VRKSDYEYLLSMPIRNLFPEGFRELCADKQKLENKLKV 306
            + GAT +               VR SDYEYLLSM I  L  E  +ELC DKQ+L+ ++  
Sbjct: 1114 VAGATEDTEENEDNSEALSSRGVRASDYEYLLSMAIGTLTLEKVQELCNDKQRLDGEVDE 1173

Query: 305  LK 300
            L+
Sbjct: 1174 LR 1175


>ref|XP_021908138.1| SKP1-like protein 1B [Carica papaya]
          Length = 155

 Score = 92.4 bits (228), Expect = 1e-19
 Identities = 46/58 (79%), Positives = 52/58 (89%)
 Frame = -1

Query: 788 IPLPNVNSKILSKVIEYSKKHVDSTSSKSDEKTADEELKAWDAEFVKVDQATLFDLIL 615
           IPLPNV SKILSKV+EY KKHV+S   K+D++TADEELK WDA+FVKVDQATLFDLIL
Sbjct: 43  IPLPNVTSKILSKVVEYCKKHVESP--KADDRTADEELKNWDADFVKVDQATLFDLIL 98


>ref|XP_021600553.1| SKP1-like protein 1B [Manihot esculenta]
 gb|OAY22590.1| hypothetical protein MANES_18G010700 [Manihot esculenta]
          Length = 157

 Score = 92.4 bits (228), Expect = 1e-19
 Identities = 52/103 (50%), Positives = 65/103 (63%)
 Frame = -1

Query: 788 IPLPNVNSKILSKVIEYSKKHVDSTSSKSDEKTADEELKAWDAEFVKVDQATLFDLILEV 609
           IPLPNV SKILSKVIEY KKHV++  S+    + D+ELKAWDAEFVKVDQATLFDLIL  
Sbjct: 43  IPLPNVTSKILSKVIEYCKKHVETPKSEDRPSSVDDELKAWDAEFVKVDQATLFDLILAA 102

Query: 608 LLEKMEYELLKFDNKVRFILSIVEGKITFSQRSEDWFLKGFTP 480
               ++  L   D+  + +  +++GK     R        FTP
Sbjct: 103 NYLNIKSLL---DHTCQTVADMIKGKTPEEIRKTFNIKNDFTP 142


>ref|XP_010920068.1| PREDICTED: DNA topoisomerase 2 [Elaeis guineensis]
          Length = 1486

 Score = 98.6 bits (244), Expect = 2e-19
 Identities = 59/123 (47%), Positives = 77/123 (62%), Gaps = 18/123 (14%)
 Frame = -1

Query: 614  EVLLEKMEYELLKFDNKVRFILSIVEGKITFSQRSE-DWFL----KGFTPFPTKTKDIDA 450
            + LL+ +E +LLK DNKVRFIL +V G+I  + R   D FL    KGF PFP K+K IDA
Sbjct: 1029 KALLDNLELDLLKLDNKVRFILGVVRGEIIVNNRKRADLFLELQQKGFAPFPKKSKGIDA 1088

Query: 449  AIEGATVN-------------GVVRKSDYEYLLSMPIRNLFPEGFRELCADKQKLENKLK 309
            AI GATV              G  R +DYEYLLSM I  L  E  +ELCA+++KLE++++
Sbjct: 1089 AIAGATVEEDGNEESPEVVNGGGARAADYEYLLSMAIGTLTWEKVQELCAERKKLEDEVE 1148

Query: 308  VLK 300
             L+
Sbjct: 1149 ELR 1151


>ref|XP_020113224.1| DNA topoisomerase 2-like [Ananas comosus]
          Length = 536

 Score = 97.8 bits (242), Expect = 2e-19
 Identities = 57/122 (46%), Positives = 74/122 (60%), Gaps = 17/122 (13%)
 Frame = -1

Query: 614 EVLLEKMEYELLKFDNKVRFILSIVEGKITFSQRSE-----DWFLKGFTPFPTKTKDIDA 450
           +VLL+ +E +LLK DNKVRFIL +V G+I  + R       +   KGFTPFP K+K ID 
Sbjct: 322 KVLLDNLEMDLLKLDNKVRFILGVVRGEIIVNNRKRAELFIELHQKGFTPFPKKSKGIDV 381

Query: 449 AIEGATVN------------GVVRKSDYEYLLSMPIRNLFPEGFRELCADKQKLENKLKV 306
           AI GAT              G VR SDYEYLLSM I  L  E  +ELC ++ KLE++++ 
Sbjct: 382 AIVGATTEEEENEESPEMGKGGVRASDYEYLLSMAIGTLTLEKVQELCGERDKLESEVEE 441

Query: 305 LK 300
           L+
Sbjct: 442 LR 443