BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon24_contig00007848
         (3907 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020267901.1| E3 ubiquitin-protein ligase UPL4-like [Aspar...  1653   0.0  
gb|PNS98590.1| hypothetical protein POPTR_016G085200v3 [Populus ...  1132   0.0  
gb|PNS98589.1| hypothetical protein POPTR_016G085200v3 [Populus ...  1109   0.0  
ref|XP_022939346.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1026   0.0  
ref|XP_023005685.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1021   0.0  
ref|XP_023539461.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1020   0.0  
gb|PIA45462.1| hypothetical protein AQUCO_01700766v1, partial [A...  1015   0.0  
gb|PIA45461.1| hypothetical protein AQUCO_01700766v1 [Aquilegia ...  1015   0.0  
ref|XP_023527254.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1014   0.0  
ref|XP_023539460.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1012   0.0  
ref|XP_023539462.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1012   0.0  
ref|XP_023539459.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1012   0.0  
ref|XP_022983502.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1012   0.0  
ref|XP_022934795.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1006   0.0  
ref|XP_023527253.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1004   0.0  
ref|XP_023527252.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1004   0.0  
ref|XP_022983501.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1001   0.0  
ref|XP_022983499.1| E3 ubiquitin-protein ligase UPL4-like isofor...  1001   0.0  
ref|XP_022934794.1| E3 ubiquitin-protein ligase UPL4-like isofor...   995   0.0  
ref|XP_022934791.1| E3 ubiquitin-protein ligase UPL4-like isofor...   995   0.0  

>ref|XP_020267901.1| E3 ubiquitin-protein ligase UPL4-like [Asparagus officinalis]
 gb|ONK67834.1| uncharacterized protein A4U43_C05F4270 [Asparagus officinalis]
          Length = 1400

 Score = 1653 bits (4281), Expect = 0.0
 Identities = 871/1260 (69%), Positives = 994/1260 (78%), Gaps = 12/1260 (0%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF++  QR+A+S  +N C++VPEDC S VMDAVP LC LLDYQD+KLVES A  LM+IVE
Sbjct: 154  FFDIRVQRVAISIAMNACRKVPEDCLSTVMDAVPTLCKLLDYQDQKLVESAATSLMKIVE 213

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
            NF HSS+LLDELCKHGVVKQSVKLISP S +ALSE T IGMIELL RLAN SLL++  LY
Sbjct: 214  NFSHSSDLLDELCKHGVVKQSVKLISPCSTVALSERTNIGMIELLARLANSSLLAVSILY 273

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E NIN+IL ++LR P LS     SH ED SRDQ++ ALKLLNQLIP +A D++DIQLV G
Sbjct: 274  ELNINSILGNLLRRPHLSHSAHNSHSEDDSRDQIFAALKLLNQLIPPMACDEKDIQLVSG 333

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            K +II +EPKLSFQFA  ILPALIQAV SGA+  I Y  VSVI NY L  TPD+LR LIK
Sbjct: 334  KNEIIANEPKLSFQFAEDILPALIQAVNSGASTRICYGSVSVILNYFLAVTPDVLRVLIK 393

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+PKFLA LLS+K+HH+LIP+L +VE+IM++LPGELL  FVKEGVSHAI V+SKQ+  
Sbjct: 394  NANIPKFLASLLSQKNHHLLIPSLLIVEVIMRELPGELLKPFVKEGVSHAINVISKQENW 453

Query: 903  YQSQLMDQQSNNLGDTDERTAADLSRCLCYAFDQSSSP-SSESKMCNLPRHTLLPLAQHV 1079
             Q+        + GDTD++T A+  RCLCYAFD  SSP SSES+ C   R  L PLAQ +
Sbjct: 454  SQT--------DTGDTDDQTIANPPRCLCYAFDPCSSPSSSESRTCRQRRCDLSPLAQRL 505

Query: 1080 MSTFFXXXXXXXXXXXXXXQKLKACCIALDKNVDSALTNCAEREDYLSQMIVEAVTALNE 1259
            MSTFF              +KLK+CC+ +D NV SAL N +E+E+YL+QM+ E V  LNE
Sbjct: 506  MSTFFTETLISSMVFSENLRKLKSCCMEIDNNVHSALPNSSEKEEYLTQMLGELVKVLNE 565

Query: 1260 GESISTFEFIESGFIRFLAHYLTNGDYLHRALDSDMSFDYLTVLKRLQTFSFTLLSGHSG 1439
            GES+STFEFIESG  RFL HYLTNGD+  R + S+ +  Y TVLKR QTF+  LLSG S 
Sbjct: 566  GESVSTFEFIESGIPRFLVHYLTNGDHFPRGVYSNKTEAYHTVLKRFQTFTSMLLSGQSW 625

Query: 1440 QHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGE 1619
            Q FPL SLV+HLQNALS+LE FP++LS+  +   R  DI A+  T HPCLQV+FV EE E
Sbjct: 626  QLFPLTSLVRHLQNALSSLESFPIILSSGYRPTPRRADISAEKPTVHPCLQVNFVTEERE 685

Query: 1620 TDLCLYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNI---KQSSRKVDSKEQVG 1790
            T+LC Y+NVLTVD SSS+D IEEFLWP                   ++S++ VDS+EQ  
Sbjct: 686  TELCNYNNVLTVDFSSSWDTIEEFLWPKVKKKMSVTKSAQETRHPGEKSAQIVDSQEQGE 745

Query: 1791 SSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTL 1970
            +S  QNNE D+ PKL+FLLDGKQLD S+TLYQAILQDKIN+DP II++++FWNEVH V  
Sbjct: 746  NSMLQNNE-DVKPKLVFLLDGKQLDHSVTLYQAILQDKINQDPAIIINREFWNEVHGVKF 804

Query: 1971 RSAKKLQLIYP------QISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSN 2132
            R AK+LQL  P      Q+ AD  QS+LS D   L    N SFFSSMLL+ELP KLERSN
Sbjct: 805  RKAKELQLRLPLKKGSFQVFADTSQSSLSCDTAGL-CRQNLSFFSSMLLSELPCKLERSN 863

Query: 2133 PAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLT 2312
            PAYD+L++LKILEG+N  SFQL SNE+SNAFAEG L N DDLKVIA SLPQTEFI+SKL 
Sbjct: 864  PAYDILYILKILEGINGFSFQLSSNEKSNAFAEGRLNNADDLKVIAPSLPQTEFISSKLN 923

Query: 2313 SKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKK 2492
            SKLEQQMHDHL LST TMPLWC Q++V+ PFLFS EARWKYF LN  G+SRN   NSEK 
Sbjct: 924  SKLEQQMHDHLVLSTTTMPLWCTQIMVSFPFLFSLEARWKYFFLNTLGISRNHMQNSEKN 983

Query: 2493 EISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPT 2672
            EIS INDRRSLS SL RKKFKVHR+NIL SA KMM WYA KKVLLEI F+NEVGTGLGPT
Sbjct: 984  EISNINDRRSLSTSLRRKKFKVHRDNILGSAVKMMNWYASKKVLLEIVFNNEVGTGLGPT 1043

Query: 2673 LEFYTLVSHVFQKVGMGMWRGDHTSTAKRL--RTSMVVSDSGFIIVHSGLFPRPWSNGMD 2846
            LEFYTLVSH  QKVGMGMWRGDHTS+  +L  R SMVVSDS F+  HSGLFPRPWS  ++
Sbjct: 1044 LEFYTLVSHELQKVGMGMWRGDHTSSGTKLHSRKSMVVSDSSFVRAHSGLFPRPWSIRVE 1103

Query: 2847 FSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEQDLDIYDIQSFDPEL 3026
              NGVSFSKVLKNFFLLGQL ARAIQDGRILD  FSRAFYKL+LE+DLDIYDIQSFDPEL
Sbjct: 1104 SLNGVSFSKVLKNFFLLGQLVARAIQDGRILDFTFSRAFYKLVLEKDLDIYDIQSFDPEL 1163

Query: 3027 GRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGD 3206
            GRT+IEFQAL KRK FLE  SGE S D     FRN RIE+LCLDF+LPGYPDY F S  D
Sbjct: 1164 GRTLIEFQALVKRKAFLE--SGELSEDGSALSFRNARIEDLCLDFALPGYPDYAFGS-SD 1220

Query: 3207 SKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGE 3386
            SKMVN+SNL EY+SLVVDATV SGISRQLESFKSGF++VFPIKALQIFTA+ELE+++ GE
Sbjct: 1221 SKMVNMSNLGEYISLVVDATVGSGISRQLESFKSGFNEVFPIKALQIFTAQELEQIVSGE 1280

Query: 3387 QDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLA 3566
            QDTWD  E Q+HI FDHGYSSTS  A+ FLE+IQEF+ RQRRAFLLFVTGAPRLPPGGLA
Sbjct: 1281 QDTWDNNEFQDHIKFDHGYSSTSPTAIMFLEVIQEFEVRQRRAFLLFVTGAPRLPPGGLA 1340

Query: 3567 ALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
            AL+PKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERM+EKLLYAI EGQGAFHLS
Sbjct: 1341 ALRPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMKEKLLYAIMEGQGAFHLS 1400


>gb|PNS98590.1| hypothetical protein POPTR_016G085200v3 [Populus trichocarpa]
          Length = 1504

 Score = 1132 bits (2928), Expect = 0.0
 Identities = 622/1271 (48%), Positives = 832/1271 (65%), Gaps = 23/1271 (1%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR+ALST++N+CK++P + FS  M+AVP+LCNLL Y+DR+LVE+ A+CL++I E
Sbjct: 241  FFSTSVQRVALSTVVNICKKLPSENFSPFMEAVPILCNLLQYEDRQLVENVAICLIKIAE 300

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                SSE+LDELCKHG++ Q+  L+  +S   LS+  Y G+I LLV+L++ S+++ R+LY
Sbjct: 301  RVSQSSEMLDELCKHGLINQATHLVQLNSRTTLSQPVYNGLIGLLVKLSSGSIVAFRTLY 360

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E NI++IL+ +    DLS      H  D   +QV+  LKLLN+L+PTVAR+    QLVL 
Sbjct: 361  ELNISSILKDLFATYDLSHGISSPHVIDGQGNQVHEVLKLLNELLPTVARNQDAQQLVLD 420

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            KE  + + P L  +F   I+P+LIQ V SGAN  + Y C+ VI   V ++  DML  L+K
Sbjct: 421  KEAFLANHPDLLHKFGSDIIPSLIQVVNSGANLYVCYGCLYVINKLVYLSKSDMLLELLK 480

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            N N   FLAG+L+RKDHHVL+ AL + E I++KLP   +NSF+KEGV  AI  L   + C
Sbjct: 481  NTNFSSFLAGVLTRKDHHVLMLALQITETILQKLPDVFVNSFIKEGVFFAIDGLLVPEKC 540

Query: 903  YQSQLMDQQSNN----LGDTDERTAADLSRCLCYAFDQSSSPS-SESKMCNLPRHTLLPL 1067
              SQL+    N     L    + ++  + RCLCYAFD   S S SE+  C L + T+  L
Sbjct: 541  --SQLIFPACNGIHLPLNSNQKSSSKVVMRCLCYAFDTGQSLSASETGTCKLEKDTVENL 598

Query: 1068 AQHVMSTFFXXXXXXXXXXXXXX-QKLKACCIALDK--NVDSALTNCAEREDYLSQMIVE 1238
             +H+  ++F               QKL+A    L    N+   + +C + E+    ++ +
Sbjct: 599  GKHIRISYFALESCDSEKGLTDILQKLRALSAELSDLMNMSVKIGSCTQDEEKCYSILCQ 658

Query: 1239 AVTALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSDMSFD-YLTVLKRLQTFSF 1415
             +  L+  E +STFEFIESG ++ L +YL NG YL   ++   +FD +  V KR + F+ 
Sbjct: 659  IMEKLDGREPVSTFEFIESGIVKILVNYLFNGKYLREKVEPQSTFDDFYVVEKRFEVFAR 718

Query: 1416 TLLSGHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQV 1595
             L S    +  PL++L+Q LQ ALS+ E FPV+LS A+K +S    IP    T +PCL+V
Sbjct: 719  LLSSSDLSEESPLSALIQKLQGALSSSENFPVILSHASKYRSSFAIIPNGRRTSYPCLRV 778

Query: 1596 HFVAEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVD 1772
             FV  EGET LC Y ++ +TVD  SS + IE FL P                 + +  V 
Sbjct: 779  RFVRGEGETCLCNYSEDPVTVDPLSSVNTIEGFLSPKVRIKGTEQIESAAQALEPAENVS 838

Query: 1773 SKEQVGSST-----------TQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDP 1919
             ++ V S +           T  + GD  PKL+F L+G+QLDR++TLYQAILQ K+  D 
Sbjct: 839  VEDIVQSPSCADDSTKSHCPTSCSNGDAMPKLVFYLEGQQLDRTLTLYQAILQQKVKADH 898

Query: 1920 DIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLL 2099
            +I  + K W +VH +T R A   +    Q      Q++   D+  + +  + +FFSSM  
Sbjct: 899  EINSTAKLWTQVHTLTYRIAVDTRDDNTQDCPSMAQNSSILDQA-VAFMQHPAFFSSMFN 957

Query: 2100 TELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSL 2279
             ELP  L++S+P  D+LF+LK LEGLNR  F L S+ER +AFAEG ++N D+L+V A+ +
Sbjct: 958  CELPSDLDKSSPTNDILFLLKSLEGLNRFIFHLMSHERIHAFAEGLIDNLDNLRVAARPV 1017

Query: 2280 PQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGV 2459
             Q EF++SKLT KLEQQM D LA+S   MP+WC QL+ +C FLFSFE R KYF L+ FG 
Sbjct: 1018 AQNEFVSSKLTEKLEQQMRDSLAVSMGGMPVWCNQLMNSCSFLFSFETRCKYFQLSAFGC 1077

Query: 2460 SRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEF 2639
             + Q   S       + DR   + SL RKKF V R+ +L SAA+MM  YA  KV +E+ +
Sbjct: 1078 QQIQIQPSSHNNSGVLRDRLPSAGSLSRKKFIVLRDQVLESAAQMMDRYAHLKVPIEVVY 1137

Query: 2640 SNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTS--TAKRLRTSMVVSDSGFIIVHSG 2813
            + EVGTGLGPTLEFYTLVS  FQK G+GMWR DH S  T + L+       SG +    G
Sbjct: 1138 NEEVGTGLGPTLEFYTLVSKEFQKSGIGMWREDHISFPTIENLQAEY----SGIVKSPFG 1193

Query: 2814 LFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEQDLD 2993
            LFPRPWS  +D S+GV FS+V+K FFLLGQ+ A+A+QDGR+LDLPF++ FYKL+L+Q+L+
Sbjct: 1194 LFPRPWSPTVDASDGVQFSEVIKKFFLLGQIVAKALQDGRVLDLPFAKVFYKLILQQELN 1253

Query: 2994 IYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPG 3173
            +YDIQSFDPELGRT++EFQAL  RK  +     E+S    D  F NTRIE+LCLDF+LPG
Sbjct: 1254 LYDIQSFDPELGRTLLEFQALVNRKKNMGLVIVENSSSTQDACFWNTRIEDLCLDFTLPG 1313

Query: 3174 YPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFT 3353
            Y DY+ S   D K+VN+ NLE YVS +VDAT+ +GISRQ+E+FKSGF++VFPIK L IFT
Sbjct: 1314 YSDYILSFDEDHKIVNMDNLEVYVSHIVDATIHTGISRQVEAFKSGFNQVFPIKHLMIFT 1373

Query: 3354 AEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVT 3533
             EELERLLCGE+D W   EL +HI FDHGY+++S P +  LEII+EF+  QRR+FL FVT
Sbjct: 1374 EEELERLLCGERDFWAFNELLDHIKFDHGYTASSPPIVNLLEIIKEFEYEQRRSFLQFVT 1433

Query: 3534 GAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYA 3713
            GAPRLP GGLA+L PKLTIV KHC N  D++LPSVMTCANYLKLPPYSSK++M+EKLLYA
Sbjct: 1434 GAPRLPTGGLASLNPKLTIVRKHCSNCEDVDLPSVMTCANYLKLPPYSSKDKMKEKLLYA 1493

Query: 3714 IREGQGAFHLS 3746
            I EGQG+FHLS
Sbjct: 1494 ITEGQGSFHLS 1504


>gb|PNS98589.1| hypothetical protein POPTR_016G085200v3 [Populus trichocarpa]
          Length = 1512

 Score = 1109 bits (2869), Expect = 0.0
 Identities = 619/1284 (48%), Positives = 827/1284 (64%), Gaps = 36/1284 (2%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR+ALST++N+CK++P + FS  M+AVP+LCNLL Y+DR+LVE+ A+CL++I E
Sbjct: 241  FFSTSVQRVALSTVVNICKKLPSENFSPFMEAVPILCNLLQYEDRQLVENVAICLIKIAE 300

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                SSE+LDELCKHG++ Q+  L+  +S   LS+  Y G+I LLV+L++ S+++ R+LY
Sbjct: 301  RVSQSSEMLDELCKHGLINQATHLVQLNSRTTLSQPVYNGLIGLLVKLSSGSIVAFRTLY 360

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E NI++IL+ +    DLS      H  D   +QV+  LKLLN+L+PTVAR+    QLVL 
Sbjct: 361  ELNISSILKDLFATYDLSHGISSPHVIDGQGNQVHEVLKLLNELLPTVARNQDAQQLVLD 420

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            KE  + + P L  +F   I+P+LIQ V SGAN  + Y C+ VI   V ++  DML  L+K
Sbjct: 421  KEAFLANHPDLLHKFGSDIIPSLIQVVNSGANLYVCYGCLYVINKLVYLSKSDMLLELLK 480

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            N N   FLAG+L+RKDHHVL+ AL + E I++KLP   +NSF+KEGV  AI  L   + C
Sbjct: 481  NTNFSSFLAGVLTRKDHHVLMLALQITETILQKLPDVFVNSFIKEGVFFAIDGLLVPEKC 540

Query: 903  YQSQLMDQQSNN----LGDTDERTAADLSRCLCYAFDQSSSPS-SESKMCNLPRHTLLPL 1067
              SQL+    N     L    + ++  + RCLCYAFD   S S SE+  C L + T+  L
Sbjct: 541  --SQLIFPACNGIHLPLNSNQKSSSKVVMRCLCYAFDTGQSLSASETGTCKLEKDTVENL 598

Query: 1068 AQHV-MSTFFXXXXXXXXXXXXXXQKLKACCIALD--KNVDSALTNCAEREDYLSQMIVE 1238
             +H+ +S F               QKL+A    L    N+   + +C + E+    ++ +
Sbjct: 599  GKHIRISYFALESCDSEKGLTDILQKLRALSAELSDLMNMSVKIGSCTQDEEKCYSILCQ 658

Query: 1239 AVTALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSDMSF-DYLTVLKRLQTFSF 1415
             +  L+  E +STFEFIESG ++ L +YL NG YL   ++   +F D+  V KR + F+ 
Sbjct: 659  IMEKLDGREPVSTFEFIESGIVKILVNYLFNGKYLREKVEPQSTFDDFYVVEKRFEVFAR 718

Query: 1416 TLLSGHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQV 1595
             L S    +  PL++L+Q LQ ALS+ E FPV+LS A+K +S    IP    T +PCL+V
Sbjct: 719  LLSSSDLSEESPLSALIQKLQGALSSSENFPVILSHASKYRSSFAIIPNGRRTSYPCLRV 778

Query: 1596 HFVAEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVD 1772
             FV  EGET LC Y ++ +TVD  SS + IE FL P                 + +  V 
Sbjct: 779  RFVRGEGETCLCNYSEDPVTVDPLSSVNTIEGFLSPKVRIKGTEQIESAAQALEPAENVS 838

Query: 1773 SKEQVGSST-----------TQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDP 1919
             ++ V S +           T  + GD  PKL+F L+G+QLDR++TLYQAILQ K+  D 
Sbjct: 839  VEDIVQSPSCADDSTKSHCPTSCSNGDAMPKLVFYLEGQQLDRTLTLYQAILQQKVKADH 898

Query: 1920 DIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLL 2099
            +I  + K W +VH +T R A   +    Q      Q++   D+  + +  + +FFSSM  
Sbjct: 899  EINSTAKLWTQVHTLTYRIAVDTRDDNTQDCPSMAQNSSILDQA-VAFMQHPAFFSSMFN 957

Query: 2100 TELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSL 2279
             ELP  L++S+P  D+LF+LK LEGLNR  F L S+ER +AFAEG ++N D+L+V A+ +
Sbjct: 958  CELPSDLDKSSPTNDILFLLKSLEGLNRFIFHLMSHERIHAFAEGLIDNLDNLRVAARPV 1017

Query: 2280 PQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGV 2459
             Q EF++SKLT KLEQQM D LA+S   MP+WC QL+ +C FLFSFE R KYF L+ FG 
Sbjct: 1018 AQNEFVSSKLTEKLEQQMRDSLAVSMGGMPVWCNQLMNSCSFLFSFETRCKYFQLSAFGC 1077

Query: 2460 SRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEF 2639
             + Q   S       + DR   + SL RKKF V R+ +L SAA+MM  YA  KV +E+ +
Sbjct: 1078 QQIQIQPSSHNNSGVLRDRLPSAGSLSRKKFIVLRDQVLESAAQMMDRYAHLKVPIEVVY 1137

Query: 2640 SNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTS--TAKRLRTSMVVSDSGFIIVHSG 2813
            + EVGTGLGPTLEFYTLVS  FQK G+GMWR DH S  T + L+       SG +    G
Sbjct: 1138 NEEVGTGLGPTLEFYTLVSKEFQKSGIGMWREDHISFPTIENLQAEY----SGIVKSPFG 1193

Query: 2814 LFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEQDLD 2993
            LFPRPWS  +D S+GV FS+V+K FFLLGQ+ A+A+QDGR+LDLPF++ FYKL+L+Q+L+
Sbjct: 1194 LFPRPWSPTVDASDGVQFSEVIKKFFLLGQIVAKALQDGRVLDLPFAKVFYKLILQQELN 1253

Query: 2994 IYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPG 3173
            +YDIQSFDPELGRT++EFQAL  RK  +     E+S    D  F NTRIE+LCLDF+LPG
Sbjct: 1254 LYDIQSFDPELGRTLLEFQALVNRKKNMGLVIVENSSSTQDACFWNTRIEDLCLDFTLPG 1313

Query: 3174 YPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFT 3353
            Y DY+ S   D K+VN+ NLE YVS +VDAT+ +GISRQ+E+FKSGF++VFPIK L IFT
Sbjct: 1314 YSDYILSFDEDHKIVNMDNLEVYVSHIVDATIHTGISRQVEAFKSGFNQVFPIKHLMIFT 1373

Query: 3354 AEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVT 3533
             EELERLLCGE+D W   EL +HI FDHGY+++S P +       EF+  QRR+FL FVT
Sbjct: 1374 EEELERLLCGERDFWAFNELLDHIKFDHGYTASSPPIVNV-----EFEYEQRRSFLQFVT 1428

Query: 3534 GAPRLPPGGLAALKPKLTIVCK-------------HCDNDADMELPSVMTCANYLKLPPY 3674
            GAPRLP GGLA+L PKLTIV K             HC N  D++LPSVMTCANYLKLPPY
Sbjct: 1429 GAPRLPTGGLASLNPKLTIVRKSLFNHLSSWCSLQHCSNCEDVDLPSVMTCANYLKLPPY 1488

Query: 3675 SSKERMREKLLYAIREGQGAFHLS 3746
            SSK++M+EKLLYAI EGQG+FHLS
Sbjct: 1489 SSKDKMKEKLLYAITEGQGSFHLS 1512


>ref|XP_022939346.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita moschata]
          Length = 1485

 Score = 1026 bits (2652), Expect = 0.0
 Identities = 580/1272 (45%), Positives = 812/1272 (63%), Gaps = 24/1272 (1%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++NVC+++P +C   ++DAVP+LCNLL Y+D +L E+ A+C+++IVE
Sbjct: 242  FFSTSIQRTALRTVVNVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVE 301

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S ELLD LC+HG+++Q + LI+ +S   LS+TTY  ++ +LV+L++ S ++ ++LY
Sbjct: 302  RVSQSCELLDGLCRHGLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLY 361

Query: 363  ESNINNILESILRNPDLSRRTPPSHCE--DISRDQVYVALKLLNQLIPTVARDDQDIQLV 536
            E NI+N L+ IL   +LS     S C   D  R+QV   LKLLN+L+PT    D + + +
Sbjct: 362  ELNISNTLKDILSAYNLSHGMSSS-CSVVDGQRNQVCEVLKLLNELLPT---GDANAEEL 417

Query: 537  LGKEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRAL 716
              K   ++  PK   +F + +LP L+Q V+SGAN  +   C+++I  +V +   DML  L
Sbjct: 418  SEKVSFLVSNPKKLQKFGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDMLVEL 477

Query: 717  IKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQD 896
            ++N N+  FLAG+ +RKDHHVL+ AL + EII++KL      SFVKEGV  AI  L   +
Sbjct: 478  LQNTNISSFLAGVFTRKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPE 537

Query: 897  GCYQS--QLMDQQSNNLGDTDERTAADLSRCLCYAFDQSS-SPSSESKMCNLPRHTLLPL 1067
               Q    +      ++G   ++++ + +RCLCYAF     S + E+  C L + ++  L
Sbjct: 538  KYKQLIFPVFTGVHPSIGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSL 596

Query: 1068 AQHVMSTFFXXXXXXXXXXXXXX-QKLKACCIALDKNVDSALTNC--AEREDYLSQMIVE 1238
            A H+ + +F               Q L+    +LD  ++ +L     A+ E+    ++ E
Sbjct: 597  AYHIRTHYFSEELCDTDKGVTDILQNLQTLSGSLDDLLNLSLIKDTPAQDEENFYALLAE 656

Query: 1239 AVTALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSDMSFDYLTVL-KRLQTFSF 1415
             ++ L  GE ISTFEFIESG ++   +YLTNG Y+ +  +  +     +++ +R + F+ 
Sbjct: 657  IMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYMRKKGEPHIISRQFSIMERRFEVFAR 716

Query: 1416 TLLSGHS--GQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCL 1589
             LLS      +  P+  L++ LQ +LS+LE FPVV S   K ++    +P      HPC+
Sbjct: 717  LLLSSAEPPSESLPVLVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCV 776

Query: 1590 QVHFVAEEGETDLCLYD-NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQ---- 1754
            +V FV  +GETDLC Y  ++ TVD  SS +AIE FLW               ++++    
Sbjct: 777  KVRFVRGDGETDLCNYTGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLREHQIK 836

Query: 1755 ----SSRKVDSKEQVGSSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPD 1922
                 S +V++ E++  S + + +G + PKL+  L+GKQL+ ++TLYQAILQ  + E+ +
Sbjct: 837  LLSNGSTEVNTDEKLQYSVSCSKKG-MKPKLLLYLEGKQLEPTLTLYQAILQQHMKEN-E 894

Query: 1923 IIVSKKFWNEVHRVTLRSAKKLQ--LIYPQISADPCQSTLSWDKTELPWHNNFSFFSSML 2096
             I   K WN+V+ +T +SA + +       +SA    +TL            FS F   L
Sbjct: 895  TISGTKLWNKVYTLTYKSAGESEDNSSNELLSASDKAATLK-----------FSSFFHGL 943

Query: 2097 LTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQS 2276
               LP  L + +PAYD+LF+L+ +EG+NR++F + S+ER  AFAEG +   D++K+   S
Sbjct: 944  DCILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDNIKLSFPS 1003

Query: 2277 LPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFG 2456
            +PQ EF+NSKLT KLEQQM D  A S   MPLWC +L+ +CPFLFSF+AR KYF +  F 
Sbjct: 1004 VPQNEFVNSKLTEKLEQQMRDFSAFSIGGMPLWCKELMDSCPFLFSFDARCKYFRIVAFA 1063

Query: 2457 VSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIE 2636
            +S  Q +     ++ T ND RS S  LPRKK  VHRN IL SA KMM  YA +KVLLE+E
Sbjct: 1064 MSHRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQKVLLEVE 1123

Query: 2637 FSNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHS-- 2810
            +  EVGTGLGPTLEFYTLV   FQK  +GMWRGDH +         ++S  G   V S  
Sbjct: 1124 YDEEVGTGLGPTLEFYTLVGREFQKFDLGMWRGDHGA---------LISIEGRETVESPF 1174

Query: 2811 GLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEQDL 2990
            GLFPRPW + +D ++ + FS+V+K F LLG++ ARAIQDGR++D+ FS+AFYKL+L Q++
Sbjct: 1175 GLFPRPWPSTLD-THELYFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYKLILGQEV 1233

Query: 2991 DIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLP 3170
             IYDIQSFDPELG  ++EFQAL  R   LES   E+S  + +  + NT IE+LCLDF++P
Sbjct: 1234 SIYDIQSFDPELGTALLEFQALVDRGKLLESVCEENSSAKLEFCYHNTNIEDLCLDFTVP 1293

Query: 3171 GYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIF 3350
            GYPDY+  S  D  MVN  NLE+YVSLVVDAT+ SGISRQ+E+FKSGF++VFPI+ LQ+F
Sbjct: 1294 GYPDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGISRQIEAFKSGFNQVFPIEHLQVF 1353

Query: 3351 TAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFV 3530
            TAEELERL+CGE D+W + EL  ++ FDHGY+++S   +  LEIIQEFD   +RAFL FV
Sbjct: 1354 TAEELERLICGEHDSWALNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQRAFLQFV 1413

Query: 3531 TGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLY 3710
            TGAPRLP GG A+L PKLTIV KH  N  D +LPSVMTCANYLKLPPYSSKE M+EKLLY
Sbjct: 1414 TGAPRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEIMKEKLLY 1473

Query: 3711 AIREGQGAFHLS 3746
            AI EGQG+FHLS
Sbjct: 1474 AITEGQGSFHLS 1485


>ref|XP_023005685.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita maxima]
          Length = 1486

 Score = 1021 bits (2639), Expect = 0.0
 Identities = 579/1272 (45%), Positives = 805/1272 (63%), Gaps = 24/1272 (1%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++NVC+++P +C   ++DAVP+LCNLL Y+D +L E+ A+C+++IVE
Sbjct: 243  FFSTSIQRTALRTVVNVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVE 302

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S ELLD LC HG+++Q + LI+ +S   LS+TTY  ++ +LV+L++ S ++ ++LY
Sbjct: 303  RVSQSCELLDGLCHHGLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLY 362

Query: 363  ESNINNILESILRNPDLSRRTPPSHCE--DISRDQVYVALKLLNQLIPTVARDDQDIQLV 536
            E NI+N L+ IL   +LS     S C   D  R+QV   LKLLN+L+PT    D + + +
Sbjct: 363  ELNISNTLKDILSAYNLSHGMSSS-CSVVDGQRNQVCEVLKLLNELLPT---GDANAEEL 418

Query: 537  LGKEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRAL 716
              K   ++  PK   +F + +LP L+Q V+SGAN      C+++I  +V +    ML  L
Sbjct: 419  SEKVSFLVSNPKKLQKFGLDVLPLLVQVVSSGANLYACCGCLTIIYKFVCLGESGMLVEL 478

Query: 717  IKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQD 896
            ++N N+  FLAG+ +RKDHHVL+ AL + EII++KL      SFVKEGV  AI  L   +
Sbjct: 479  LQNTNISSFLAGVFTRKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPE 538

Query: 897  GCYQS--QLMDQQSNNLGDTDERTAADLSRCLCYAFDQSS-SPSSESKMCNLPRHTLLPL 1067
               Q    +      + G   ++++ + +RCLCYAF     S + E+  C L + ++  L
Sbjct: 539  KYKQLIFPVFTGVHPSFGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSL 597

Query: 1068 AQHVMSTFFXXXXXXXXXXXXXX-QKLKACCIALDKNVDSALTNC--AEREDYLSQMIVE 1238
            A H+ + +F               Q L+    +LD  ++ +L     A+ E+    ++ E
Sbjct: 598  AYHIRTHYFSEELCDTDKGVTDILQNLQTLSGSLDDLLNLSLIKDTPAQDEENFYALLAE 657

Query: 1239 AVTALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSDM-SFDYLTVLKRLQTFSF 1415
             ++ L  GE ISTFEFIESG ++   +YLTNG Y+ +  +  + S  +  + +R + F+ 
Sbjct: 658  IMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYMRKKGEPQIISRQFAIMERRFEVFAR 717

Query: 1416 TLLSGHS--GQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCL 1589
             LLS      +  P+  L++ LQ +LS+LE FPVV S   K ++    +P      HPC+
Sbjct: 718  LLLSSSEPPSESLPVLVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCV 777

Query: 1590 QVHFVAEEGETDLCLYD-NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQ---- 1754
            +V FV  +GETDLC Y  ++ TVD  SS +AIE FLWP              ++++    
Sbjct: 778  KVRFVRGDGETDLCNYTGDIHTVDPFSSLNAIEGFLWPKVGTKKAEQSFEADSLREHQIK 837

Query: 1755 ----SSRKVDSKEQVGSSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPD 1922
                 S +V++ E++  S + + +G + PKL+  L+GKQL+ ++TLYQAILQ  + E+ +
Sbjct: 838  LLSNGSTEVNTDEKLQYSASCSKKG-MKPKLLLYLEGKQLEPTLTLYQAILQQHMKEN-E 895

Query: 1923 IIVSKKFWNEVHRVTLRSAKKLQ--LIYPQISADPCQSTLSWDKTELPWHNNFSFFSSML 2096
                 K WN+V+ +T +SA + +        SA    +TL            FS F   L
Sbjct: 896  TTSGTKLWNQVYTLTYKSAGEREDNSSNKLFSASDKAATLQ-----------FSSFFHSL 944

Query: 2097 LTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQS 2276
               LP  L + +PAYD+LF+L+ +EG+NR++F + S+ER  AFAEG +   D++K+   S
Sbjct: 945  DCILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDNIKLSVTS 1004

Query: 2277 LPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFG 2456
            +PQ EF+NSKLT KLEQQM D  A S   MPLWC +L+ +CPFLFSF+AR KYF +  F 
Sbjct: 1005 VPQNEFVNSKLTEKLEQQMRDFSAFSVGGMPLWCKELMDSCPFLFSFDARCKYFQIVAFA 1064

Query: 2457 VSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIE 2636
            +S  Q +     ++ T ND RS S  LPRKK  VHRN IL SA KMM  YA +KVLLE+E
Sbjct: 1065 MSHRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQKVLLEVE 1124

Query: 2637 FSNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHS-- 2810
            +  EVGTGLGPTLEFYTLV   FQK  +GMWRGDH +         ++S  G   V S  
Sbjct: 1125 YDEEVGTGLGPTLEFYTLVGREFQKYDLGMWRGDHGA---------LISLEGRETVESPF 1175

Query: 2811 GLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEQDL 2990
            GLFPRPW + +D +  + FS+V+K F LLG++ ARAIQDGR++D+ FS+AFYKL+L Q++
Sbjct: 1176 GLFPRPWPSTLD-TRELYFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYKLILGQEV 1234

Query: 2991 DIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLP 3170
             IYDIQSFDPELG  ++EFQAL  R   LES   E+S  + +  + NT IE+LCLDF++P
Sbjct: 1235 SIYDIQSFDPELGTALLEFQALVDRGKLLESVCEENSSAKLEFCYHNTNIEDLCLDFTVP 1294

Query: 3171 GYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIF 3350
            GYPDY+  S  D  MVN  NLE+YVSLVVDAT+ SGISRQ+E+FKSGF++VFPI+ LQ+F
Sbjct: 1295 GYPDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGISRQIEAFKSGFNQVFPIEHLQVF 1354

Query: 3351 TAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFV 3530
            T EELERL+CGE D+W + EL  ++ FDHGY+++S   +  LEIIQEFD   +RAFL FV
Sbjct: 1355 TEEELERLICGEHDSWALNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQRAFLQFV 1414

Query: 3531 TGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLY 3710
            TGAPRLP GG A+L PKLTIV KH  N  D +LPSVMTCANYLKLPPYSSKE M+EKLLY
Sbjct: 1415 TGAPRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEIMKEKLLY 1474

Query: 3711 AIREGQGAFHLS 3746
            AI EGQG+FHLS
Sbjct: 1475 AITEGQGSFHLS 1486


>ref|XP_023539461.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita pepo
            subsp. pepo]
          Length = 1485

 Score = 1020 bits (2638), Expect = 0.0
 Identities = 576/1270 (45%), Positives = 808/1270 (63%), Gaps = 22/1270 (1%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++NVC+++P +C   ++DAVP+LCNLL Y+D +L E+ A+C+++IVE
Sbjct: 242  FFSTSIQRTALRTVVNVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVE 301

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S ELLD LC HG+++Q + LI+ +S   LS+TTY  ++ +LV+L++ S ++ ++LY
Sbjct: 302  RVSQSCELLDGLCHHGLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLY 361

Query: 363  ESNINNILESILRNPDLSRRTPPSHCE--DISRDQVYVALKLLNQLIPTVARDDQDIQLV 536
            E NI+N L+ IL   +LS     S C   D  R+QV   LKLLN+L+PT    D + + +
Sbjct: 362  ELNISNTLKDILSAYNLSHGMSSS-CSVVDGQRNQVCEVLKLLNELLPT---GDANAEEL 417

Query: 537  LGKEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRAL 716
              K   ++  PK   +F + +LP L+Q V+SGAN  +   C+++I  +V +    ML  L
Sbjct: 418  SEKVYFLVSNPKKLQKFGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESGMLVEL 477

Query: 717  IKNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQD 896
            ++N N+  FLAG+ +RKDHHVL+ AL + EII++KL      SFVKEGV  AI  L   +
Sbjct: 478  LQNTNISSFLAGVFTRKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPE 537

Query: 897  GCYQS--QLMDQQSNNLGDTDERTAADLSRCLCYAFDQSS-SPSSESKMCNLPRHTLLPL 1067
               Q    +      ++G   ++++ + +RCLCYAF     S + E+  C L + ++  L
Sbjct: 538  KYKQLIFPVFTGVHPSIGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSL 596

Query: 1068 AQHVMSTFFXXXXXXXXXXXXXX-QKLKACCIALDKNVDSALTN--CAEREDYLSQMIVE 1238
            A H+ + +F               Q L+    +LD  ++ +L     A+ E+    ++ E
Sbjct: 597  AYHIRTHYFSEELCDTDKGVTDILQNLQTLAGSLDDLLNLSLIKETPAQDEENCYALLAE 656

Query: 1239 AVTALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSDMSFDYLTVL-KRLQTFSF 1415
             ++ L  GE ISTFEFIESG ++   +YLTNG Y+ +  +  +     +++ +R + F+ 
Sbjct: 657  IMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYMRKKGEPQIISRQFSIMERRFEVFAR 716

Query: 1416 TLLSGHS--GQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCL 1589
             LLS      +  P+  L++ LQ +LS+LE FPVV S   K ++    +P      HPC+
Sbjct: 717  LLLSSAEPPSESLPVLVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCV 776

Query: 1590 QVHFVAEEGETDLCLYD-NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQ---- 1754
            +V FV  +GETDLC Y  ++ TVD  SS +AIE FLW               ++++    
Sbjct: 777  KVRFVRGDGETDLCNYTGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLREHQIK 836

Query: 1755 ----SSRKVDSKEQVGSSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPD 1922
                 S +VD+ E++  S + + +G + PKL+  L+GKQL+ ++TLYQAILQ  + E  +
Sbjct: 837  LLSNGSTEVDTDEKLQYSVSCSKKG-MKPKLLLYLEGKQLEPTLTLYQAILQQHMKES-E 894

Query: 1923 IIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLT 2102
             +   K WN+V+ +T +SA +        S D   + L +  ++      FS F   L  
Sbjct: 895  TVSGTKLWNQVYTLTYKSAGE--------SEDNSSNEL-FSASDKAATLKFSSFFHGLDC 945

Query: 2103 ELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLP 2282
             LP  L + +PAYD+LF+L+ +EG+NR++F + S+ER  AFAEG +   D++K+   S+P
Sbjct: 946  ILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDNIKLSVPSVP 1005

Query: 2283 QTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVS 2462
            Q EF+NSKLT KLEQQM D  A S   MPLWC +L+ +CPFLFSF+AR KYF +  F +S
Sbjct: 1006 QNEFVNSKLTEKLEQQMRDFSAFSIGGMPLWCKELMDSCPFLFSFDARCKYFRIVAFSMS 1065

Query: 2463 RNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFS 2642
              Q +     ++ T ND RS S  LPRKK  VHRN IL SA KMM  YA +KVLLE+E+ 
Sbjct: 1066 HRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQKVLLEVEYD 1125

Query: 2643 NEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHS--GL 2816
             EVGTGLGPTLEFYTLV   FQK  +GMWRGDH +         ++S  G   V S  GL
Sbjct: 1126 EEVGTGLGPTLEFYTLVGREFQKYDLGMWRGDHGA---------LISIEGRETVESPFGL 1176

Query: 2817 FPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEQDLDI 2996
            FPRPW + +D ++ + FS+V+K F LLG++ ARAIQDGR++D+ FS+AFYKL+L Q++ I
Sbjct: 1177 FPRPWPSTLD-THELYFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYKLILGQEVSI 1235

Query: 2997 YDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGY 3176
            YDIQSFDPELG  ++EFQAL  R   LE    E+S  + +  + NT IE+LCLDF++PGY
Sbjct: 1236 YDIQSFDPELGTALLEFQALVDRGKLLEFVCEENSSAKLEFCYHNTNIEDLCLDFTVPGY 1295

Query: 3177 PDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTA 3356
            PDY+  S  D  MVN  NLE+YVSLVVDAT+ SGISRQ+++FKSGF++VFPI+ LQ+FTA
Sbjct: 1296 PDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGISRQIDAFKSGFNQVFPIEHLQVFTA 1355

Query: 3357 EELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTG 3536
            EELERL+CGE D+W + EL  ++ FDHGY+++S   +  LEIIQEFD   +RAFL FVTG
Sbjct: 1356 EELERLICGEHDSWALNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQRAFLQFVTG 1415

Query: 3537 APRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAI 3716
            APRLP GG A+L PKLTIV KH  N  D +LPSVMTCANYLKLPPYSSKE M+EKLLYAI
Sbjct: 1416 APRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEIMKEKLLYAI 1475

Query: 3717 REGQGAFHLS 3746
             EGQG+FHLS
Sbjct: 1476 TEGQGSFHLS 1485


>gb|PIA45462.1| hypothetical protein AQUCO_01700766v1, partial [Aquilegia coerulea]
          Length = 1426

 Score = 1015 bits (2624), Expect = 0.0
 Identities = 575/1258 (45%), Positives = 799/1258 (63%), Gaps = 10/1258 (0%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR+ ++ I N+CK++P +    VM+AVP+LCNLL Y+D+KLVE+ A CL+ IV+
Sbjct: 214  FFASSVQRVGITIIANICKKLPSEFSPQVMEAVPLLCNLLYYEDQKLVENVATCLIWIVK 273

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
               HS ++LDE+CK G+ +Q   LI+ +    L++  Y  +I LL RLA+ S+ +  SL 
Sbjct: 274  KASHSPDMLDEVCKQGLTQQVAHLIASNVGNILNQPIYTDLIGLLSRLASGSITASNSLL 333

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E NI+N +  ILR+ DLS   PPS   ++        +KLL  L+P ++ +      V  
Sbjct: 334  EHNISNTMSLILRSYDLSHSKPPSLIHEV--------IKLLYVLLPPLSTNCNGKTEVSN 385

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
             ++II  +P L  QF   +LP L+Q V SGAN  I Y C+SV+   +  +T DML  L+K
Sbjct: 386  MDRIIAHQPGLLQQFGADVLPVLVQIVNSGANTHICYGCLSVVNKIIHFSTSDMLVDLLK 445

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+  FLAG+++RK+ H+L+ AL +V+ +M++LP    NSF+KEGV + I  L   + C
Sbjct: 446  NANISSFLAGIIARKELHLLMLALTIVKNVMQELPDAFFNSFIKEGVVYTIDALITPEEC 505

Query: 903  YQSQLMDQQSNNL--GDTDERTAADLSRCLCYAFDQSSSPSSESKMCNLPRHTLLPLAQH 1076
             Q  L   +   L         A D   CLCY +D   S  SE+++C L   ++  LA++
Sbjct: 506  PQFILQTSEGAQLLSRSNQRMPANDSVGCLCYVYDIDQS--SENEICKLEETSVHTLAKY 563

Query: 1077 VMSTFFXXXXXXXXXXXXXX-QKLKACCIALD--KNVDSALTNCAEREDYLSQMIVEAVT 1247
            + +T+F               +KL+  C  L   +N+  +  NC E+E  LS  + + + 
Sbjct: 564  IRTTYFTTKSGCSELKSISLLKKLRNLCAVLSDKENMSVSNDNC-EQEKNLSYTLGQILE 622

Query: 1248 ALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSDMSFDYLTVL-KRLQTFSFTLL 1424
             L+  E +STFEFIESG +R L  YL+NG Y +R +D +   ++L VL KR + F+   L
Sbjct: 623  ELSGEEPVSTFEFIESGIVRSLVDYLSNGCYRNRVVDPNELLNHLLVLQKRYEVFAKLCL 682

Query: 1425 S--GHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVH 1598
            S   H  +  PL  L+  LQ+ALS LE FPVVL   +K K     IP + +T HPCL+V 
Sbjct: 683  SPPSHPLKDKPLGILLGKLQSALSLLENFPVVLHDVSKPKETYVTIPFERTTFHPCLKVR 742

Query: 1599 FVAEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDS 1775
            F+ EEG+T LC Y  +V++++  +S +AIE +LWP               +K  +    +
Sbjct: 743  FIKEEGDTSLCDYVSDVVSIEAFTSINAIERYLWP--------------RVKHEAES--A 786

Query: 1776 KEQVGSSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEV 1955
             E+   +T      + T KL+F LD  QLD+++TLYQAILQ K+  + D+ V   FW +V
Sbjct: 787  TEECAVATEDLKSSNDTQKLLFYLDKGQLDQTLTLYQAILQLKMETENDLSVGPSFWKQV 846

Query: 1956 HRVTL-RSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSN 2132
            + +   +   + ++  P +  +   S+  W   ++ +  N   FSSML +E P   ++S+
Sbjct: 847  YEIKYTKDTGQKKINIPDVHHNS-GSSREWSHPQI-YLQNAMCFSSMLFSEPPLSFKKSS 904

Query: 2133 PAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLT 2312
              Y++L +LKILE LNR +  L  +ER ++FAEG  ++ D+L V    +PQTEF+N KLT
Sbjct: 905  TIYEILTLLKILEVLNRSASHLVYHERISSFAEGCDDSLDNLIVPVCGVPQTEFVNLKLT 964

Query: 2313 SKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKK 2492
             KLEQQM D   +ST ++P WC+QL+ A PFLF FE R KYF + +   S  Q  N    
Sbjct: 965  GKLEQQMQDASVVSTGSIPSWCVQLMEAFPFLFGFETRCKYFQMTLVNSSEVQPDN---- 1020

Query: 2493 EISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPT 2672
                I+DR+S ++  PRKKFKV R+ IL SAA+ M  + G+K +LE+E+  EVGTG GPT
Sbjct: 1021 ----IDDRQSQASGSPRKKFKVCRSRILESAAQAMSSHVGRKAILEVEYPEEVGTGQGPT 1076

Query: 2673 LEFYTLVSHVFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFS 2852
            +EF+TLVS  FQKVGMGMWRGD   T+    ++  V  SGF++   GLFPRPWS      
Sbjct: 1077 MEFFTLVSQEFQKVGMGMWRGDPKLTSM---SNSEVDSSGFVVAPFGLFPRPWS-ASSAP 1132

Query: 2853 NGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEQDLDIYDIQSFDPELGR 3032
            N    S+V+K F LLGQL A+A+QDGR+LDLP S + YKL+LEQDL++YDI SFD  +GR
Sbjct: 1133 NETQLSEVVKKFVLLGQLVAKALQDGRVLDLPLSCSLYKLILEQDLNLYDIFSFDHGVGR 1192

Query: 3033 TMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSK 3212
             ++EFQAL  RK  L+S S    + R D  F+NTRIE+LCLDF+LPGYPDY  +S+   +
Sbjct: 1193 ALVEFQALVDRKKVLDSVS----VKRSDSYFQNTRIEDLCLDFTLPGYPDYKLTSVDGHE 1248

Query: 3213 MVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQD 3392
            MVN+SNL+EYVS  +DATV +GI RQ+E+FK GF++V P+K+L+IFTAEEL++LLCGEQ+
Sbjct: 1249 MVNMSNLQEYVSSTLDATVNNGICRQVEAFKLGFNQVLPLKSLKIFTAEELDQLLCGEQN 1308

Query: 3393 TWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAAL 3572
             W+  EL +HI FDHGY+++S   +K LEI+QEF+  QRRAFL FVTGAPRLPPGGLAAL
Sbjct: 1309 AWNYNELLDHIKFDHGYTASSPTIIKLLEIMQEFEYNQRRAFLQFVTGAPRLPPGGLAAL 1368

Query: 3573 KPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
             PKLTIVCK C   AD +LPSVMTCANYLKLPPYSSKE M+E+LLYAI EGQG+F LS
Sbjct: 1369 NPKLTIVCKPCKGGADGDLPSVMTCANYLKLPPYSSKEIMKERLLYAITEGQGSFLLS 1426


>gb|PIA45461.1| hypothetical protein AQUCO_01700766v1 [Aquilegia coerulea]
          Length = 1221

 Score = 1015 bits (2624), Expect = 0.0
 Identities = 575/1258 (45%), Positives = 799/1258 (63%), Gaps = 10/1258 (0%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR+ ++ I N+CK++P +    VM+AVP+LCNLL Y+D+KLVE+ A CL+ IV+
Sbjct: 9    FFASSVQRVGITIIANICKKLPSEFSPQVMEAVPLLCNLLYYEDQKLVENVATCLIWIVK 68

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
               HS ++LDE+CK G+ +Q   LI+ +    L++  Y  +I LL RLA+ S+ +  SL 
Sbjct: 69   KASHSPDMLDEVCKQGLTQQVAHLIASNVGNILNQPIYTDLIGLLSRLASGSITASNSLL 128

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E NI+N +  ILR+ DLS   PPS   ++        +KLL  L+P ++ +      V  
Sbjct: 129  EHNISNTMSLILRSYDLSHSKPPSLIHEV--------IKLLYVLLPPLSTNCNGKTEVSN 180

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
             ++II  +P L  QF   +LP L+Q V SGAN  I Y C+SV+   +  +T DML  L+K
Sbjct: 181  MDRIIAHQPGLLQQFGADVLPVLVQIVNSGANTHICYGCLSVVNKIIHFSTSDMLVDLLK 240

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+  FLAG+++RK+ H+L+ AL +V+ +M++LP    NSF+KEGV + I  L   + C
Sbjct: 241  NANISSFLAGIIARKELHLLMLALTIVKNVMQELPDAFFNSFIKEGVVYTIDALITPEEC 300

Query: 903  YQSQLMDQQSNNL--GDTDERTAADLSRCLCYAFDQSSSPSSESKMCNLPRHTLLPLAQH 1076
             Q  L   +   L         A D   CLCY +D   S  SE+++C L   ++  LA++
Sbjct: 301  PQFILQTSEGAQLLSRSNQRMPANDSVGCLCYVYDIDQS--SENEICKLEETSVHTLAKY 358

Query: 1077 VMSTFFXXXXXXXXXXXXXX-QKLKACCIALD--KNVDSALTNCAEREDYLSQMIVEAVT 1247
            + +T+F               +KL+  C  L   +N+  +  NC E+E  LS  + + + 
Sbjct: 359  IRTTYFTTKSGCSELKSISLLKKLRNLCAVLSDKENMSVSNDNC-EQEKNLSYTLGQILE 417

Query: 1248 ALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSDMSFDYLTVL-KRLQTFSFTLL 1424
             L+  E +STFEFIESG +R L  YL+NG Y +R +D +   ++L VL KR + F+   L
Sbjct: 418  ELSGEEPVSTFEFIESGIVRSLVDYLSNGCYRNRVVDPNELLNHLLVLQKRYEVFAKLCL 477

Query: 1425 S--GHSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVH 1598
            S   H  +  PL  L+  LQ+ALS LE FPVVL   +K K     IP + +T HPCL+V 
Sbjct: 478  SPPSHPLKDKPLGILLGKLQSALSLLENFPVVLHDVSKPKETYVTIPFERTTFHPCLKVR 537

Query: 1599 FVAEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDS 1775
            F+ EEG+T LC Y  +V++++  +S +AIE +LWP               +K  +    +
Sbjct: 538  FIKEEGDTSLCDYVSDVVSIEAFTSINAIERYLWP--------------RVKHEAES--A 581

Query: 1776 KEQVGSSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEV 1955
             E+   +T      + T KL+F LD  QLD+++TLYQAILQ K+  + D+ V   FW +V
Sbjct: 582  TEECAVATEDLKSSNDTQKLLFYLDKGQLDQTLTLYQAILQLKMETENDLSVGPSFWKQV 641

Query: 1956 HRVTL-RSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSN 2132
            + +   +   + ++  P +  +   S+  W   ++ +  N   FSSML +E P   ++S+
Sbjct: 642  YEIKYTKDTGQKKINIPDVHHNS-GSSREWSHPQI-YLQNAMCFSSMLFSEPPLSFKKSS 699

Query: 2133 PAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLT 2312
              Y++L +LKILE LNR +  L  +ER ++FAEG  ++ D+L V    +PQTEF+N KLT
Sbjct: 700  TIYEILTLLKILEVLNRSASHLVYHERISSFAEGCDDSLDNLIVPVCGVPQTEFVNLKLT 759

Query: 2313 SKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKK 2492
             KLEQQM D   +ST ++P WC+QL+ A PFLF FE R KYF + +   S  Q  N    
Sbjct: 760  GKLEQQMQDASVVSTGSIPSWCVQLMEAFPFLFGFETRCKYFQMTLVNSSEVQPDN---- 815

Query: 2493 EISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPT 2672
                I+DR+S ++  PRKKFKV R+ IL SAA+ M  + G+K +LE+E+  EVGTG GPT
Sbjct: 816  ----IDDRQSQASGSPRKKFKVCRSRILESAAQAMSSHVGRKAILEVEYPEEVGTGQGPT 871

Query: 2673 LEFYTLVSHVFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFS 2852
            +EF+TLVS  FQKVGMGMWRGD   T+    ++  V  SGF++   GLFPRPWS      
Sbjct: 872  MEFFTLVSQEFQKVGMGMWRGDPKLTSM---SNSEVDSSGFVVAPFGLFPRPWS-ASSAP 927

Query: 2853 NGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEQDLDIYDIQSFDPELGR 3032
            N    S+V+K F LLGQL A+A+QDGR+LDLP S + YKL+LEQDL++YDI SFD  +GR
Sbjct: 928  NETQLSEVVKKFVLLGQLVAKALQDGRVLDLPLSCSLYKLILEQDLNLYDIFSFDHGVGR 987

Query: 3033 TMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSK 3212
             ++EFQAL  RK  L+S S    + R D  F+NTRIE+LCLDF+LPGYPDY  +S+   +
Sbjct: 988  ALVEFQALVDRKKVLDSVS----VKRSDSYFQNTRIEDLCLDFTLPGYPDYKLTSVDGHE 1043

Query: 3213 MVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQD 3392
            MVN+SNL+EYVS  +DATV +GI RQ+E+FK GF++V P+K+L+IFTAEEL++LLCGEQ+
Sbjct: 1044 MVNMSNLQEYVSSTLDATVNNGICRQVEAFKLGFNQVLPLKSLKIFTAEELDQLLCGEQN 1103

Query: 3393 TWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAAL 3572
             W+  EL +HI FDHGY+++S   +K LEI+QEF+  QRRAFL FVTGAPRLPPGGLAAL
Sbjct: 1104 AWNYNELLDHIKFDHGYTASSPTIIKLLEIMQEFEYNQRRAFLQFVTGAPRLPPGGLAAL 1163

Query: 3573 KPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
             PKLTIVCK C   AD +LPSVMTCANYLKLPPYSSKE M+E+LLYAI EGQG+F LS
Sbjct: 1164 NPKLTIVCKPCKGGADGDLPSVMTCANYLKLPPYSSKEIMKERLLYAITEGQGSFLLS 1221


>ref|XP_023527254.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita pepo
            subsp. pepo]
          Length = 1489

 Score = 1014 bits (2623), Expect = 0.0
 Identities = 569/1265 (44%), Positives = 799/1265 (63%), Gaps = 17/1265 (1%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++N+CK++P +C   +++AVP+LCNL  + D +LVE+ A C+++I E
Sbjct: 242  FFSSSIQRTALRTVVNICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAE 301

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +LV+L++ S+++ ++LY
Sbjct: 302  CAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLY 361

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E +I+N L+ IL   +LS         D  R+QV   LKLLN+L+PT   +D   + +  
Sbjct: 362  ELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSE 418

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            K   +   PK   +F + ILP L+Q V+SGA   +   C+++I  ++ +   DML  L++
Sbjct: 419  KVSFLASNPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQ 478

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKEGV  AI  L   +  
Sbjct: 479  NANISSFLVGVFTRKDHHVLMLALKITEIILQKLCSIFLKSFVKEGVYFAIGALITPEKY 538

Query: 903  YQS--QLMDQQSNNLGDTDERTAADLSRCLCYAFDQSSSPS-SESKMCNLPRHTLLPLAQ 1073
             Q    +      + G   + T  +  RCLCYAF      S +E+  C L   ++  LA 
Sbjct: 539  KQLIFPVFAGVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLAN 597

Query: 1074 HVMSTFFXXXXXXXXXXXXXX-QKLKACCIALDKNVDSALTNC--AEREDYLSQMIVEAV 1244
            H+ + +F               Q L     ALD  ++ +L     A+ E+    ++ E +
Sbjct: 598  HIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYALLAEIM 657

Query: 1245 TALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSD-MSFDYLTVLKRLQTFSFTL 1421
            + L  GE ISTFEFIESG ++   +YLTNG YL +  +   +S  +  + +R + F+  L
Sbjct: 658  SKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLL 717

Query: 1422 LSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQV 1595
             S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++    +P      HPC++V
Sbjct: 718  FSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKV 777

Query: 1596 HFVAEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVD 1772
             FV  +GETDLC +  ++LTVD  SS +AIE FLWP              ++++   K+ 
Sbjct: 778  RFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLL 837

Query: 1773 SKEQVGSSTTQNNEGDIT-------PKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIV 1931
            S   V  ST + ++G  +       PKL+  L+GKQL+ ++TLYQ ILQ  I E+ + I 
Sbjct: 838  SNVSVEVSTDEKSQGSASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQQHIKEN-EAIS 896

Query: 1932 SKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELP 2111
              K W++V+ +  + A        ++  + C    S        H + SFF  +L  +LP
Sbjct: 897  GTKVWSQVYTIMYKRAG-------EVEDNSCNQFFSASDKGATLHFS-SFFCGILDCDLP 948

Query: 2112 FKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTE 2291
              L R +P YD+LF+L+I+EG+NR++F + S+ER  AF+EG +   D++K+   S+ Q E
Sbjct: 949  SDLARESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEGRISTLDNIKLSVPSVSQNE 1008

Query: 2292 FINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQ 2471
            F+NSKLT KLEQQM D  A+S   MPLWC +L+ +CPFLFSFEAR KYF + +FG+ + Q
Sbjct: 1009 FVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQ-Q 1067

Query: 2472 THNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEV 2651
             +     ++ T N  RS S  LPRKK  V R+ IL SAAKMM  YA +KVLLE+E+  EV
Sbjct: 1068 PYVRSYSDLGTSNGVRSSSGGLPRKKALVLRDKILLSAAKMMDQYAHQKVLLEVEYDEEV 1127

Query: 2652 GTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHSGLFPRPW 2831
            GTGLGPTLEFYTLVS  FQK G+GMWRGDH +       S+ +     I    GLFPRPW
Sbjct: 1128 GTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIPG--KSLNIEGRETIESPFGLFPRPW 1185

Query: 2832 SNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEQDLDIYDIQS 3011
             + +D    + FS+V+K F L+GQ+ A+AIQDGR++D+ FS+AFYKL+L Q++ IYDIQS
Sbjct: 1186 LSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILGQEVSIYDIQS 1244

Query: 3012 FDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVF 3191
            FDPELG  ++EFQAL  R   LES   E+S  R +  + +T IE+LCLDF+LPGYPD   
Sbjct: 1245 FDPELGTVLLEFQALVNRSKLLESVCEENSSSRLEFCYHDTNIEDLCLDFTLPGYPDCRL 1304

Query: 3192 SSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELER 3371
            +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+ LQ+FTAEELER
Sbjct: 1305 TSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFNQVFPIEHLQVFTAEELER 1364

Query: 3372 LLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLP 3551
            L+CGEQD+W + +L ++I FDHGY+++S   +  LEIIQ+FD  Q+RAFL FVTGAPRLP
Sbjct: 1365 LICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAFLQFVTGAPRLP 1424

Query: 3552 PGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQG 3731
             GG A+L PKLTIV KH  N  D +LPSVMTCANYLKLPPYSSKE M+EKLLYAI EGQG
Sbjct: 1425 SGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQG 1484

Query: 3732 AFHLS 3746
            +FHLS
Sbjct: 1485 SFHLS 1489


>ref|XP_023539460.1| E3 ubiquitin-protein ligase UPL4-like isoform X2 [Cucurbita pepo
            subsp. pepo]
          Length = 1500

 Score = 1012 bits (2616), Expect = 0.0
 Identities = 577/1294 (44%), Positives = 804/1294 (62%), Gaps = 46/1294 (3%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++NVC+++P +C   ++DAVP+LCNLL Y+D +L E+ A+C+++IVE
Sbjct: 232  FFSTSIQRTALRTVVNVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVE 291

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S ELLD LC HG+++Q + LI+ +S   LS+TTY  ++ +LV+L++ S ++ ++LY
Sbjct: 292  RVSQSCELLDGLCHHGLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLY 351

Query: 363  ESNINNILESILRNPDLSRRTPPS-HCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVL 539
            E NI+N L+ IL   +LS     S    D  R+QV   LKLLN+L+PT    D + + + 
Sbjct: 352  ELNISNTLKDILSAYNLSHGMSSSCSVVDGQRNQVCEVLKLLNELLPT---GDANAEELS 408

Query: 540  GKEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALI 719
             K   ++  PK   +F + +LP L+Q V+SGAN  +   C+++I  +V +    ML  L+
Sbjct: 409  EKVYFLVSNPKKLQKFGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESGMLVELL 468

Query: 720  KNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDG 899
            +N N+  FLAG+ +RKDHHVL+ AL + EII++KL      SFVKEGV  AI  L   + 
Sbjct: 469  QNTNISSFLAGVFTRKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPEK 528

Query: 900  CYQ--SQLMDQQSNNLGDTDERTAADLSRCLCYAF-DQSSSPSSESKMCNLPRHTLLPLA 1070
              Q    +      ++G   ++++ + +RCLCYAF     S + E+  C L + ++  LA
Sbjct: 529  YKQLIFPVFTGVHPSIGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSLA 587

Query: 1071 QHVMSTFF-XXXXXXXXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEA 1241
             H+ + +F               Q L+    +LD   N+       A+ E+    ++ E 
Sbjct: 588  YHIRTHYFSEELCDTDKGVTDILQNLQTLAGSLDDLLNLSLIKETPAQDEENCYALLAEI 647

Query: 1242 VTALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSD-MSFDYLTVLKRLQTFSFT 1418
            ++ L  GE ISTFEFIESG ++   +YLTNG Y+ +  +   +S  +  + +R + F+  
Sbjct: 648  MSKLKCGEPISTFEFIESGIVKSFINYLTNGQYMRKKGEPQIISRQFSIMERRFEVFARL 707

Query: 1419 LLSGHS--GQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQ 1592
            LLS      +  P+  L++ LQ +LS+LE FPVV S   K ++    +P      HPC++
Sbjct: 708  LLSSAEPPSESLPVLVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCVK 767

Query: 1593 VHFVAEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIK------ 1751
            V FV  +GETDLC Y  ++ TVD  SS +AIE FLW               +++      
Sbjct: 768  VRFVRGDGETDLCNYTGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLREHQIKL 827

Query: 1752 ---------------------------QSSRKVDSKEQVGSSTTQNNEGDITPKLIFLLD 1850
                                       Q S +VD+ E++  S + + +G + PKL+  L+
Sbjct: 828  LSNVCSGWGVNPKLVGSDSMSTDLSETQGSTEVDTDEKLQYSVSCSKKG-MKPKLLLYLE 886

Query: 1851 GKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQS 2030
            GKQL+ ++TLYQAILQ  + E  + +   K WN+V+ +T +SA +        S D   +
Sbjct: 887  GKQLEPTLTLYQAILQQHMKES-ETVSGTKLWNQVYTLTYKSAGE--------SEDNSSN 937

Query: 2031 TLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNE 2210
             L +  ++      FS F   L   LP  L + +PAYD+LF+L+ +EG+NR++F + S+E
Sbjct: 938  EL-FSASDKAATLKFSSFFHGLDCILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHE 996

Query: 2211 RSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLV 2390
            R  AFAEG +   D++K+   S+PQ EF+NSKLT KLEQQM D  A S   MPLWC +L+
Sbjct: 997  RIRAFAEGRINTLDNIKLSVPSVPQNEFVNSKLTEKLEQQMRDFSAFSIGGMPLWCKELM 1056

Query: 2391 VACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNN 2570
             +CPFLFSF+AR KYF +  F +S  Q +     ++ T ND RS S  LPRKK  VHRN 
Sbjct: 1057 DSCPFLFSFDARCKYFRIVAFSMSHRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNK 1116

Query: 2571 ILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTST 2750
            IL SA KMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLV   FQK  +GMWRGDH   
Sbjct: 1117 ILDSAVKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVGREFQKYDLGMWRGDH--- 1173

Query: 2751 AKRLRTSMVVSDSGFIIVHS--GLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQ 2924
                    ++S  G   V S  GLFPRPW + +D ++ + FS+V+K F LLG++ ARAIQ
Sbjct: 1174 ------GALISIEGRETVESPFGLFPRPWPSTLD-THELYFSEVVKKFVLLGKIVARAIQ 1226

Query: 2925 DGRILDLPFSRAFYKLMLEQDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSL 3104
            DGR++D+ FS+AFYKL+L Q++ IYDIQSFDPELG  ++EFQAL  R   LE    E+S 
Sbjct: 1227 DGRVMDIYFSKAFYKLILGQEVSIYDIQSFDPELGTALLEFQALVDRGKLLEFVCEENSS 1286

Query: 3105 DRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGIS 3284
             + +  + NT IE+LCLDF++PGYPDY+  S  D  MVN  NLE+YVSLVVDAT+ SGIS
Sbjct: 1287 AKLEFCYHNTNIEDLCLDFTVPGYPDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGIS 1346

Query: 3285 RQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPA 3464
            RQ+++FKSGF++VFPI+ LQ+FTAEELERL+CGE D+W + EL  ++ FDHGY+++S   
Sbjct: 1347 RQIDAFKSGFNQVFPIEHLQVFTAEELERLICGEHDSWALNELLENVKFDHGYTASSPSI 1406

Query: 3465 MKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMT 3644
            +  LEIIQEFD   +RAFL FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMT
Sbjct: 1407 VNLLEIIQEFDNEHQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMT 1466

Query: 3645 CANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
            CANYLKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1467 CANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1500


>ref|XP_023539462.1| E3 ubiquitin-protein ligase UPL4-like isoform X4 [Cucurbita pepo
            subsp. pepo]
          Length = 1475

 Score = 1012 bits (2616), Expect = 0.0
 Identities = 577/1294 (44%), Positives = 804/1294 (62%), Gaps = 46/1294 (3%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++NVC+++P +C   ++DAVP+LCNLL Y+D +L E+ A+C+++IVE
Sbjct: 207  FFSTSIQRTALRTVVNVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVE 266

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S ELLD LC HG+++Q + LI+ +S   LS+TTY  ++ +LV+L++ S ++ ++LY
Sbjct: 267  RVSQSCELLDGLCHHGLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLY 326

Query: 363  ESNINNILESILRNPDLSRRTPPS-HCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVL 539
            E NI+N L+ IL   +LS     S    D  R+QV   LKLLN+L+PT    D + + + 
Sbjct: 327  ELNISNTLKDILSAYNLSHGMSSSCSVVDGQRNQVCEVLKLLNELLPT---GDANAEELS 383

Query: 540  GKEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALI 719
             K   ++  PK   +F + +LP L+Q V+SGAN  +   C+++I  +V +    ML  L+
Sbjct: 384  EKVYFLVSNPKKLQKFGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESGMLVELL 443

Query: 720  KNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDG 899
            +N N+  FLAG+ +RKDHHVL+ AL + EII++KL      SFVKEGV  AI  L   + 
Sbjct: 444  QNTNISSFLAGVFTRKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPEK 503

Query: 900  CYQ--SQLMDQQSNNLGDTDERTAADLSRCLCYAF-DQSSSPSSESKMCNLPRHTLLPLA 1070
              Q    +      ++G   ++++ + +RCLCYAF     S + E+  C L + ++  LA
Sbjct: 504  YKQLIFPVFTGVHPSIGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSLA 562

Query: 1071 QHVMSTFF-XXXXXXXXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEA 1241
             H+ + +F               Q L+    +LD   N+       A+ E+    ++ E 
Sbjct: 563  YHIRTHYFSEELCDTDKGVTDILQNLQTLAGSLDDLLNLSLIKETPAQDEENCYALLAEI 622

Query: 1242 VTALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSD-MSFDYLTVLKRLQTFSFT 1418
            ++ L  GE ISTFEFIESG ++   +YLTNG Y+ +  +   +S  +  + +R + F+  
Sbjct: 623  MSKLKCGEPISTFEFIESGIVKSFINYLTNGQYMRKKGEPQIISRQFSIMERRFEVFARL 682

Query: 1419 LLSGHS--GQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQ 1592
            LLS      +  P+  L++ LQ +LS+LE FPVV S   K ++    +P      HPC++
Sbjct: 683  LLSSAEPPSESLPVLVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCVK 742

Query: 1593 VHFVAEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIK------ 1751
            V FV  +GETDLC Y  ++ TVD  SS +AIE FLW               +++      
Sbjct: 743  VRFVRGDGETDLCNYTGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLREHQIKL 802

Query: 1752 ---------------------------QSSRKVDSKEQVGSSTTQNNEGDITPKLIFLLD 1850
                                       Q S +VD+ E++  S + + +G + PKL+  L+
Sbjct: 803  LSNVCSGWGVNPKLVGSDSMSTDLSETQGSTEVDTDEKLQYSVSCSKKG-MKPKLLLYLE 861

Query: 1851 GKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQS 2030
            GKQL+ ++TLYQAILQ  + E  + +   K WN+V+ +T +SA +        S D   +
Sbjct: 862  GKQLEPTLTLYQAILQQHMKES-ETVSGTKLWNQVYTLTYKSAGE--------SEDNSSN 912

Query: 2031 TLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNE 2210
             L +  ++      FS F   L   LP  L + +PAYD+LF+L+ +EG+NR++F + S+E
Sbjct: 913  EL-FSASDKAATLKFSSFFHGLDCILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHE 971

Query: 2211 RSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLV 2390
            R  AFAEG +   D++K+   S+PQ EF+NSKLT KLEQQM D  A S   MPLWC +L+
Sbjct: 972  RIRAFAEGRINTLDNIKLSVPSVPQNEFVNSKLTEKLEQQMRDFSAFSIGGMPLWCKELM 1031

Query: 2391 VACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNN 2570
             +CPFLFSF+AR KYF +  F +S  Q +     ++ T ND RS S  LPRKK  VHRN 
Sbjct: 1032 DSCPFLFSFDARCKYFRIVAFSMSHRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNK 1091

Query: 2571 ILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTST 2750
            IL SA KMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLV   FQK  +GMWRGDH   
Sbjct: 1092 ILDSAVKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVGREFQKYDLGMWRGDH--- 1148

Query: 2751 AKRLRTSMVVSDSGFIIVHS--GLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQ 2924
                    ++S  G   V S  GLFPRPW + +D ++ + FS+V+K F LLG++ ARAIQ
Sbjct: 1149 ------GALISIEGRETVESPFGLFPRPWPSTLD-THELYFSEVVKKFVLLGKIVARAIQ 1201

Query: 2925 DGRILDLPFSRAFYKLMLEQDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSL 3104
            DGR++D+ FS+AFYKL+L Q++ IYDIQSFDPELG  ++EFQAL  R   LE    E+S 
Sbjct: 1202 DGRVMDIYFSKAFYKLILGQEVSIYDIQSFDPELGTALLEFQALVDRGKLLEFVCEENSS 1261

Query: 3105 DRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGIS 3284
             + +  + NT IE+LCLDF++PGYPDY+  S  D  MVN  NLE+YVSLVVDAT+ SGIS
Sbjct: 1262 AKLEFCYHNTNIEDLCLDFTVPGYPDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGIS 1321

Query: 3285 RQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPA 3464
            RQ+++FKSGF++VFPI+ LQ+FTAEELERL+CGE D+W + EL  ++ FDHGY+++S   
Sbjct: 1322 RQIDAFKSGFNQVFPIEHLQVFTAEELERLICGEHDSWALNELLENVKFDHGYTASSPSI 1381

Query: 3465 MKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMT 3644
            +  LEIIQEFD   +RAFL FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMT
Sbjct: 1382 VNLLEIIQEFDNEHQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMT 1441

Query: 3645 CANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
            CANYLKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1442 CANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1475


>ref|XP_023539459.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita pepo
            subsp. pepo]
          Length = 1510

 Score = 1012 bits (2616), Expect = 0.0
 Identities = 577/1294 (44%), Positives = 804/1294 (62%), Gaps = 46/1294 (3%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++NVC+++P +C   ++DAVP+LCNLL Y+D +L E+ A+C+++IVE
Sbjct: 242  FFSTSIQRTALRTVVNVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVE 301

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S ELLD LC HG+++Q + LI+ +S   LS+TTY  ++ +LV+L++ S ++ ++LY
Sbjct: 302  RVSQSCELLDGLCHHGLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLY 361

Query: 363  ESNINNILESILRNPDLSRRTPPS-HCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVL 539
            E NI+N L+ IL   +LS     S    D  R+QV   LKLLN+L+PT    D + + + 
Sbjct: 362  ELNISNTLKDILSAYNLSHGMSSSCSVVDGQRNQVCEVLKLLNELLPT---GDANAEELS 418

Query: 540  GKEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALI 719
             K   ++  PK   +F + +LP L+Q V+SGAN  +   C+++I  +V +    ML  L+
Sbjct: 419  EKVYFLVSNPKKLQKFGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESGMLVELL 478

Query: 720  KNANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDG 899
            +N N+  FLAG+ +RKDHHVL+ AL + EII++KL      SFVKEGV  AI  L   + 
Sbjct: 479  QNTNISSFLAGVFTRKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPEK 538

Query: 900  CYQ--SQLMDQQSNNLGDTDERTAADLSRCLCYAF-DQSSSPSSESKMCNLPRHTLLPLA 1070
              Q    +      ++G   ++++ + +RCLCYAF     S + E+  C L + ++  LA
Sbjct: 539  YKQLIFPVFTGVHPSIGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSLA 597

Query: 1071 QHVMSTFF-XXXXXXXXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEA 1241
             H+ + +F               Q L+    +LD   N+       A+ E+    ++ E 
Sbjct: 598  YHIRTHYFSEELCDTDKGVTDILQNLQTLAGSLDDLLNLSLIKETPAQDEENCYALLAEI 657

Query: 1242 VTALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSD-MSFDYLTVLKRLQTFSFT 1418
            ++ L  GE ISTFEFIESG ++   +YLTNG Y+ +  +   +S  +  + +R + F+  
Sbjct: 658  MSKLKCGEPISTFEFIESGIVKSFINYLTNGQYMRKKGEPQIISRQFSIMERRFEVFARL 717

Query: 1419 LLSGHS--GQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQ 1592
            LLS      +  P+  L++ LQ +LS+LE FPVV S   K ++    +P      HPC++
Sbjct: 718  LLSSAEPPSESLPVLVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCVK 777

Query: 1593 VHFVAEEGETDLCLY-DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIK------ 1751
            V FV  +GETDLC Y  ++ TVD  SS +AIE FLW               +++      
Sbjct: 778  VRFVRGDGETDLCNYTGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLREHQIKL 837

Query: 1752 ---------------------------QSSRKVDSKEQVGSSTTQNNEGDITPKLIFLLD 1850
                                       Q S +VD+ E++  S + + +G + PKL+  L+
Sbjct: 838  LSNVCSGWGVNPKLVGSDSMSTDLSETQGSTEVDTDEKLQYSVSCSKKG-MKPKLLLYLE 896

Query: 1851 GKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQS 2030
            GKQL+ ++TLYQAILQ  + E  + +   K WN+V+ +T +SA +        S D   +
Sbjct: 897  GKQLEPTLTLYQAILQQHMKES-ETVSGTKLWNQVYTLTYKSAGE--------SEDNSSN 947

Query: 2031 TLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNE 2210
             L +  ++      FS F   L   LP  L + +PAYD+LF+L+ +EG+NR++F + S+E
Sbjct: 948  EL-FSASDKAATLKFSSFFHGLDCILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHE 1006

Query: 2211 RSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLV 2390
            R  AFAEG +   D++K+   S+PQ EF+NSKLT KLEQQM D  A S   MPLWC +L+
Sbjct: 1007 RIRAFAEGRINTLDNIKLSVPSVPQNEFVNSKLTEKLEQQMRDFSAFSIGGMPLWCKELM 1066

Query: 2391 VACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNN 2570
             +CPFLFSF+AR KYF +  F +S  Q +     ++ T ND RS S  LPRKK  VHRN 
Sbjct: 1067 DSCPFLFSFDARCKYFRIVAFSMSHRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNK 1126

Query: 2571 ILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTST 2750
            IL SA KMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLV   FQK  +GMWRGDH   
Sbjct: 1127 ILDSAVKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVGREFQKYDLGMWRGDH--- 1183

Query: 2751 AKRLRTSMVVSDSGFIIVHS--GLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQ 2924
                    ++S  G   V S  GLFPRPW + +D ++ + FS+V+K F LLG++ ARAIQ
Sbjct: 1184 ------GALISIEGRETVESPFGLFPRPWPSTLD-THELYFSEVVKKFVLLGKIVARAIQ 1236

Query: 2925 DGRILDLPFSRAFYKLMLEQDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSL 3104
            DGR++D+ FS+AFYKL+L Q++ IYDIQSFDPELG  ++EFQAL  R   LE    E+S 
Sbjct: 1237 DGRVMDIYFSKAFYKLILGQEVSIYDIQSFDPELGTALLEFQALVDRGKLLEFVCEENSS 1296

Query: 3105 DRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGIS 3284
             + +  + NT IE+LCLDF++PGYPDY+  S  D  MVN  NLE+YVSLVVDAT+ SGIS
Sbjct: 1297 AKLEFCYHNTNIEDLCLDFTVPGYPDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGIS 1356

Query: 3285 RQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPA 3464
            RQ+++FKSGF++VFPI+ LQ+FTAEELERL+CGE D+W + EL  ++ FDHGY+++S   
Sbjct: 1357 RQIDAFKSGFNQVFPIEHLQVFTAEELERLICGEHDSWALNELLENVKFDHGYTASSPSI 1416

Query: 3465 MKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMT 3644
            +  LEIIQEFD   +RAFL FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMT
Sbjct: 1417 VNLLEIIQEFDNEHQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMT 1476

Query: 3645 CANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
            CANYLKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1477 CANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1510


>ref|XP_022983502.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita maxima]
          Length = 1489

 Score = 1012 bits (2616), Expect = 0.0
 Identities = 567/1265 (44%), Positives = 799/1265 (63%), Gaps = 17/1265 (1%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++N+CK++P +C   +++AVP+LCNL  + D +LVE+ A C+++I E
Sbjct: 242  FFSSSIQRTALRTVVNICKKLPSECPLTLIEAVPILCNLFQHDDGELVENVARCMIKIAE 301

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +LV+L++ S+++ ++LY
Sbjct: 302  CAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLY 361

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E +I+N L+ IL   +LS         D  R+QV   LKLLN+L+PT   +D   + +  
Sbjct: 362  ELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSE 418

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            K   +   PK   +F + ILP L+Q V+SGA   +   C+++I  ++ +   DML  L++
Sbjct: 419  KVSFLAINPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQ 478

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKEGV  AI  L   +  
Sbjct: 479  NANISSFLVGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEKY 538

Query: 903  YQS--QLMDQQSNNLGDTDERTAADLSRCLCYAFDQSSSPS-SESKMCNLPRHTLLPLAQ 1073
             Q    +      + G   + T  +  RCLCYAF      S +E+  C L + ++  LA 
Sbjct: 539  KQLIFPVFAGVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDKDSVYSLAN 597

Query: 1074 HVMSTFFXXXXXXXXXXXXXX-QKLKACCIALDKNVDSALTNC--AEREDYLSQMIVEAV 1244
            H+ + +F               Q L     ALD  ++ +L     A+ E+    ++ E +
Sbjct: 598  HIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYTLLAEIM 657

Query: 1245 TALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSD-MSFDYLTVLKRLQTFSFTL 1421
            + L  GE ISTFEFIESG ++   +YLTNG YL +  +   +S  +  + +R + F+  L
Sbjct: 658  SKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLL 717

Query: 1422 LSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQV 1595
             S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++    +P      HPC++V
Sbjct: 718  FSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKV 777

Query: 1596 HFVAEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVD 1772
             FV  +GETDLC +  ++LTVD  SS +AIE FLWP              ++++   K  
Sbjct: 778  RFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVTRKKAEQSSEADSLREHQVKPL 837

Query: 1773 SKEQVGSSTTQNNEGDIT-------PKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIV 1931
            S   V  ST + ++G  +       PKL+  L+GKQL+ ++TLYQ ILQ  I E+ + I 
Sbjct: 838  SNVSVEVSTDEKSQGSASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQQHIKEN-EAIS 896

Query: 1932 SKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELP 2111
              K W++V+ +  +SA        ++  + C    S        H + SFF  +L  +LP
Sbjct: 897  GTKVWSQVYTIMYKSAG-------EVEDNSCNQFFSASDKGATLHFS-SFFCGILDCDLP 948

Query: 2112 FKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTE 2291
              L + +P YD+LF+L+I+EG+NR++F + S+ER  AF+EG +   +++K+   S+ Q E
Sbjct: 949  SDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEGRISTLNNIKLSVPSVSQNE 1008

Query: 2292 FINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQ 2471
            F+NSKLT KLEQQM D  A+S   MPLWC +L+ +CPFLFSFEAR KYF + +FG+ + Q
Sbjct: 1009 FVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQ-Q 1067

Query: 2472 THNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEV 2651
             +     ++ T N  RS S   PRKK  V RN IL SAAKMM  YA +KVLLE+E+  EV
Sbjct: 1068 PYVRSYSDLGTSNGVRSSSGGFPRKKALVLRNQILLSAAKMMDQYAHQKVLLEVEYDEEV 1127

Query: 2652 GTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHSGLFPRPW 2831
            GTGLGPTLEFYTLVS  FQK G+GMWRGDH +       S+ +     I    GLFPRPW
Sbjct: 1128 GTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIPG--KSLNIEGRETIESPFGLFPRPW 1185

Query: 2832 SNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEQDLDIYDIQS 3011
             + +D    + FS+V+K F L+GQ+ A+AIQDGR++D+ FS+AFYKL+L Q++ IYDIQS
Sbjct: 1186 LSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILGQEVSIYDIQS 1244

Query: 3012 FDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVF 3191
            FDPELG  ++EFQAL  R   LES   E+S  + +  + +T IE+LCLDF+LPGYPD   
Sbjct: 1245 FDPELGTVLLEFQALVNRSKLLESVCEENSSSKLEFCYHDTNIEDLCLDFTLPGYPDCRL 1304

Query: 3192 SSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELER 3371
            +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+ LQ+FTAEELER
Sbjct: 1305 TSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFNQVFPIEHLQVFTAEELER 1364

Query: 3372 LLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLP 3551
            L+CGEQD+W + +L ++I FDHGY+++S   +  LEIIQ+FD  Q+RAFL FVTGAPRLP
Sbjct: 1365 LICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAFLQFVTGAPRLP 1424

Query: 3552 PGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQG 3731
             GG A+L PKLTIV KH  N  D +LPSVMTCANYLKLPPYSSKE M+EKLLYAI EGQG
Sbjct: 1425 SGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQG 1484

Query: 3732 AFHLS 3746
            +FHLS
Sbjct: 1485 SFHLS 1489


>ref|XP_022934795.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita moschata]
          Length = 1489

 Score = 1006 bits (2602), Expect = 0.0
 Identities = 564/1265 (44%), Positives = 795/1265 (62%), Gaps = 17/1265 (1%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++N+CK++P +C   +++AVP+LCNL  + D +LVE+ A C+++I E
Sbjct: 242  FFSSSIQRTALRTVVNICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAE 301

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +LV+L++ S+++ ++LY
Sbjct: 302  CAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLY 361

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E +I+N L+ IL   +LS         D  R+QV   LKLLN+L+PT   +D   + +  
Sbjct: 362  ELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSE 418

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            K   +   PK   +F + ILP L+Q V+SGA   +   C+++I  ++ +   DML  L++
Sbjct: 419  KVSFLASNPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQ 478

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKEGV  AI  L   +  
Sbjct: 479  NANISSFLVGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEKY 538

Query: 903  YQS--QLMDQQSNNLGDTDERTAADLSRCLCYAFDQSSSPS-SESKMCNLPRHTLLPLAQ 1073
             Q    +      + G   + T  +  RCLCYAF      S +E+  C L   ++  LA 
Sbjct: 539  KQLIFPVFASVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLAN 597

Query: 1074 HVMSTFFXXXXXXXXXXXXXX-QKLKACCIALDKNVDSALTNCA--EREDYLSQMIVEAV 1244
            H+ + +F               Q L     ALD  ++ +L      + E+    ++ E +
Sbjct: 598  HIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTPGQDEEKFYALLAEIM 657

Query: 1245 TALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALDSD-MSFDYLTVLKRLQTFSFTL 1421
            + L  GE ISTFEFIESG ++   +YLTNG YL +  +   +S  +  + +R + F+  L
Sbjct: 658  SKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLL 717

Query: 1422 LSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQV 1595
             S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++    +P      HPC++V
Sbjct: 718  FSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKV 777

Query: 1596 HFVAEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVD 1772
             FV  +GETDLC +  ++LTVD  SS +AIE FLWP              ++++   K+ 
Sbjct: 778  RFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLL 837

Query: 1773 SKEQVGSSTTQNNEGDIT-------PKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIV 1931
            S   V  ST + + G  +       PKL+  L+GKQL+ ++TLYQ ILQ  I E+ + I 
Sbjct: 838  SNASVEVSTDEKSLGSASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQRHIKEN-EAIS 896

Query: 1932 SKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELP 2111
              K W++V+ +  + A        ++  + C    S        H + SFF  +L  +LP
Sbjct: 897  GTKVWSQVYTIMYKRAG-------EVEDNSCNQFFSGSDKGATLHFS-SFFCGILDCDLP 948

Query: 2112 FKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTE 2291
              L + +P YD+LF+L+I+EG+NR++F + S+ER  AF+EG +   D++K+   S+ Q E
Sbjct: 949  SDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEGRISTLDNIKLSVPSVSQNE 1008

Query: 2292 FINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQ 2471
            F+NSKLT KLEQQM D  A+S   MPLWC +L+ +CPFLFSFEAR KYF + +FG+ + Q
Sbjct: 1009 FVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQ-Q 1067

Query: 2472 THNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEV 2651
             +     ++ T N  RS S  LPRKK  V R+ IL SAAKMM  YA +KVLLE+E+  EV
Sbjct: 1068 PYVRSYSDLGTSNGVRSSSGGLPRKKALVLRDKILLSAAKMMDQYAHQKVLLEVEYDEEV 1127

Query: 2652 GTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHSGLFPRPW 2831
            GTGLGPTLEFYTLVS  FQK G+GMWRGDH +       S+ +     I    GLFPRPW
Sbjct: 1128 GTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIPG--KSLNIEGRETIESPFGLFPRPW 1185

Query: 2832 SNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSRAFYKLMLEQDLDIYDIQS 3011
             + +D    + FS+V+K F L+GQ+ A+AIQDGR++D+ FS+AFYKL+L Q++ IYDIQS
Sbjct: 1186 LSTVDIGE-LQFSEVIKRFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILGQEVSIYDIQS 1244

Query: 3012 FDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVF 3191
            FDPELG  ++EFQAL  R   LES   E+S  + +  + +T IE+LCLDF+LPGYPD   
Sbjct: 1245 FDPELGTVLLEFQALVNRSKLLESVCEENSSSKLEFCYHDTNIEDLCLDFTLPGYPDCRL 1304

Query: 3192 SSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELER 3371
            +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+ LQ+FTAEELER
Sbjct: 1305 TSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFNQVFPIEHLQVFTAEELER 1364

Query: 3372 LLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLP 3551
            L+CGEQD+W + +L ++I FDHGY+++S   +  LEIIQ+FD  Q+RAFL FVTGAPRLP
Sbjct: 1365 LICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAFLQFVTGAPRLP 1424

Query: 3552 PGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQG 3731
             GG  +L PKLTIV KH  N  D +LPSVMTCANYLKLPPYSSKE  +EKLLYAI EGQG
Sbjct: 1425 SGGFTSLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYSSKEITKEKLLYAITEGQG 1484

Query: 3732 AFHLS 3746
            +FHLS
Sbjct: 1485 SFHLS 1489


>ref|XP_023527253.1| E3 ubiquitin-protein ligase UPL4-like isoform X2 [Cucurbita pepo
            subsp. pepo]
          Length = 1504

 Score = 1004 bits (2595), Expect = 0.0
 Identities = 572/1290 (44%), Positives = 800/1290 (62%), Gaps = 42/1290 (3%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++N+CK++P +C   +++AVP+LCNL  + D +LVE+ A C+++I E
Sbjct: 232  FFSSSIQRTALRTVVNICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAE 291

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +LV+L++ S+++ ++LY
Sbjct: 292  CAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLY 351

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E +I+N L+ IL   +LS         D  R+QV   LKLLN+L+PT   +D   + +  
Sbjct: 352  ELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSE 408

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            K   +   PK   +F + ILP L+Q V+SGA   +   C+++I  ++ +   DML  L++
Sbjct: 409  KVSFLASNPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQ 468

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKEGV  AI  L   +  
Sbjct: 469  NANISSFLVGVFTRKDHHVLMLALKITEIILQKLCSIFLKSFVKEGVYFAIGALITPEKY 528

Query: 903  YQ--SQLMDQQSNNLGDTDERTAADLSRCLCYAFDQSS-SPSSESKMCNLPRHTLLPLAQ 1073
             Q    +      + G   + T  +  RCLCYAF     S  +E+  C L   ++  LA 
Sbjct: 529  KQLIFPVFAGVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLAN 587

Query: 1074 HVMSTFF-XXXXXXXXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAV 1244
            H+ + +F               Q L     ALD   N+       A+ E+    ++ E +
Sbjct: 588  HIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYALLAEIM 647

Query: 1245 TALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALD-SDMSFDYLTVLKRLQTFSFTL 1421
            + L  GE ISTFEFIESG ++   +YLTNG YL +  +   +S  +  + +R + F+  L
Sbjct: 648  SKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLL 707

Query: 1422 LSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQV 1595
             S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++    +P      HPC++V
Sbjct: 708  FSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKV 767

Query: 1596 HFVAEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVD 1772
             FV  +GETDLC +  ++LTVD  SS +AIE FLWP              ++++   K+ 
Sbjct: 768  RFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLL 827

Query: 1773 S---------KEQVGS----------------STTQNNEGDIT-------PKLIFLLDGK 1856
            S          E VGS                ST + ++G  +       PKL+  L+GK
Sbjct: 828  SNVCSYFGVNSELVGSNSKSSDLPEIEVSVEVSTDEKSQGSASSSKKGTKPKLLLYLEGK 887

Query: 1857 QLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTL 2036
            QL+ ++TLYQ ILQ  I E+ + I   K W++V+ +  + A        ++  + C    
Sbjct: 888  QLEPTLTLYQTILQQHIKEN-EAISGTKVWSQVYTIMYKRA-------GEVEDNSCNQFF 939

Query: 2037 SWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERS 2216
            S        H + SFF  +L  +LP  L R +P YD+LF+L+I+EG+NR++F + S+ER 
Sbjct: 940  SASDKGATLHFS-SFFCGILDCDLPSDLARESPVYDVLFLLRIIEGMNRMAFHIMSHERI 998

Query: 2217 NAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVA 2396
             AF+EG +   D++K+   S+ Q EF+NSKLT KLEQQM D  A+S   MPLWC +L+ +
Sbjct: 999  RAFSEGRISTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDS 1058

Query: 2397 CPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNIL 2576
            CPFLFSFEAR KYF + +FG+ + Q +     ++ T N  RS S  LPRKK  V R+ IL
Sbjct: 1059 CPFLFSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGLPRKKALVLRDKIL 1117

Query: 2577 ASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAK 2756
             SAAKMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  FQK G+GMWRGDH +   
Sbjct: 1118 LSAAKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP 1177

Query: 2757 RLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRI 2936
                S+ +     I    GLFPRPW + +D    + FS+V+K F L+GQ+ A+AIQDGR+
Sbjct: 1178 --GKSLNIEGRETIESPFGLFPRPWLSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRV 1234

Query: 2937 LDLPFSRAFYKLMLEQDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFD 3116
            +D+ FS+AFYKL+L Q++ IYDIQSFDPELG  ++EFQAL  R   LES   E+S  R +
Sbjct: 1235 MDIYFSKAFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSRLE 1294

Query: 3117 PIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLE 3296
              + +T IE+LCLDF+LPGYPD   +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E
Sbjct: 1295 FCYHDTNIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIE 1354

Query: 3297 SFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFL 3476
            +FKSGF++VFPI+ LQ+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S   +  L
Sbjct: 1355 AFKSGFNQVFPIEHLQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLL 1414

Query: 3477 EIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANY 3656
            EIIQ+FD  Q+RAFL FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMTCANY
Sbjct: 1415 EIIQDFDNEQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANY 1474

Query: 3657 LKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
            LKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1475 LKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1504


>ref|XP_023527252.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita pepo
            subsp. pepo]
          Length = 1514

 Score = 1004 bits (2595), Expect = 0.0
 Identities = 572/1290 (44%), Positives = 800/1290 (62%), Gaps = 42/1290 (3%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++N+CK++P +C   +++AVP+LCNL  + D +LVE+ A C+++I E
Sbjct: 242  FFSSSIQRTALRTVVNICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAE 301

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +LV+L++ S+++ ++LY
Sbjct: 302  CAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLY 361

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E +I+N L+ IL   +LS         D  R+QV   LKLLN+L+PT   +D   + +  
Sbjct: 362  ELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSE 418

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            K   +   PK   +F + ILP L+Q V+SGA   +   C+++I  ++ +   DML  L++
Sbjct: 419  KVSFLASNPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQ 478

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKEGV  AI  L   +  
Sbjct: 479  NANISSFLVGVFTRKDHHVLMLALKITEIILQKLCSIFLKSFVKEGVYFAIGALITPEKY 538

Query: 903  YQ--SQLMDQQSNNLGDTDERTAADLSRCLCYAFDQSS-SPSSESKMCNLPRHTLLPLAQ 1073
             Q    +      + G   + T  +  RCLCYAF     S  +E+  C L   ++  LA 
Sbjct: 539  KQLIFPVFAGVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLAN 597

Query: 1074 HVMSTFF-XXXXXXXXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAV 1244
            H+ + +F               Q L     ALD   N+       A+ E+    ++ E +
Sbjct: 598  HIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYALLAEIM 657

Query: 1245 TALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALD-SDMSFDYLTVLKRLQTFSFTL 1421
            + L  GE ISTFEFIESG ++   +YLTNG YL +  +   +S  +  + +R + F+  L
Sbjct: 658  SKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLL 717

Query: 1422 LSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQV 1595
             S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++    +P      HPC++V
Sbjct: 718  FSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKV 777

Query: 1596 HFVAEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVD 1772
             FV  +GETDLC +  ++LTVD  SS +AIE FLWP              ++++   K+ 
Sbjct: 778  RFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLL 837

Query: 1773 S---------KEQVGS----------------STTQNNEGDIT-------PKLIFLLDGK 1856
            S          E VGS                ST + ++G  +       PKL+  L+GK
Sbjct: 838  SNVCSYFGVNSELVGSNSKSSDLPEIEVSVEVSTDEKSQGSASSSKKGTKPKLLLYLEGK 897

Query: 1857 QLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTL 2036
            QL+ ++TLYQ ILQ  I E+ + I   K W++V+ +  + A        ++  + C    
Sbjct: 898  QLEPTLTLYQTILQQHIKEN-EAISGTKVWSQVYTIMYKRA-------GEVEDNSCNQFF 949

Query: 2037 SWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERS 2216
            S        H + SFF  +L  +LP  L R +P YD+LF+L+I+EG+NR++F + S+ER 
Sbjct: 950  SASDKGATLHFS-SFFCGILDCDLPSDLARESPVYDVLFLLRIIEGMNRMAFHIMSHERI 1008

Query: 2217 NAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVA 2396
             AF+EG +   D++K+   S+ Q EF+NSKLT KLEQQM D  A+S   MPLWC +L+ +
Sbjct: 1009 RAFSEGRISTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDS 1068

Query: 2397 CPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNIL 2576
            CPFLFSFEAR KYF + +FG+ + Q +     ++ T N  RS S  LPRKK  V R+ IL
Sbjct: 1069 CPFLFSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGLPRKKALVLRDKIL 1127

Query: 2577 ASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAK 2756
             SAAKMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  FQK G+GMWRGDH +   
Sbjct: 1128 LSAAKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP 1187

Query: 2757 RLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRI 2936
                S+ +     I    GLFPRPW + +D    + FS+V+K F L+GQ+ A+AIQDGR+
Sbjct: 1188 --GKSLNIEGRETIESPFGLFPRPWLSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRV 1244

Query: 2937 LDLPFSRAFYKLMLEQDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFD 3116
            +D+ FS+AFYKL+L Q++ IYDIQSFDPELG  ++EFQAL  R   LES   E+S  R +
Sbjct: 1245 MDIYFSKAFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSRLE 1304

Query: 3117 PIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLE 3296
              + +T IE+LCLDF+LPGYPD   +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E
Sbjct: 1305 FCYHDTNIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIE 1364

Query: 3297 SFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFL 3476
            +FKSGF++VFPI+ LQ+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S   +  L
Sbjct: 1365 AFKSGFNQVFPIEHLQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLL 1424

Query: 3477 EIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANY 3656
            EIIQ+FD  Q+RAFL FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMTCANY
Sbjct: 1425 EIIQDFDNEQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANY 1484

Query: 3657 LKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
            LKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1485 LKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1514


>ref|XP_022983501.1| E3 ubiquitin-protein ligase UPL4-like isoform X2 [Cucurbita maxima]
          Length = 1504

 Score = 1001 bits (2588), Expect = 0.0
 Identities = 570/1290 (44%), Positives = 800/1290 (62%), Gaps = 42/1290 (3%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++N+CK++P +C   +++AVP+LCNL  + D +LVE+ A C+++I E
Sbjct: 232  FFSSSIQRTALRTVVNICKKLPSECPLTLIEAVPILCNLFQHDDGELVENVARCMIKIAE 291

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +LV+L++ S+++ ++LY
Sbjct: 292  CAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLY 351

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E +I+N L+ IL   +LS         D  R+QV   LKLLN+L+PT   +D   + +  
Sbjct: 352  ELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSE 408

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            K   +   PK   +F + ILP L+Q V+SGA   +   C+++I  ++ +   DML  L++
Sbjct: 409  KVSFLAINPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQ 468

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKEGV  AI  L   +  
Sbjct: 469  NANISSFLVGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEKY 528

Query: 903  YQ--SQLMDQQSNNLGDTDERTAADLSRCLCYAFDQSS-SPSSESKMCNLPRHTLLPLAQ 1073
             Q    +      + G   + T  +  RCLCYAF     S  +E+  C L + ++  LA 
Sbjct: 529  KQLIFPVFAGVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDKDSVYSLAN 587

Query: 1074 HVMSTFF-XXXXXXXXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAV 1244
            H+ + +F               Q L     ALD   N+       A+ E+    ++ E +
Sbjct: 588  HIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYTLLAEIM 647

Query: 1245 TALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALD-SDMSFDYLTVLKRLQTFSFTL 1421
            + L  GE ISTFEFIESG ++   +YLTNG YL +  +   +S  +  + +R + F+  L
Sbjct: 648  SKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLL 707

Query: 1422 LSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQV 1595
             S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++    +P      HPC++V
Sbjct: 708  FSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKV 767

Query: 1596 HFVAEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVD 1772
             FV  +GETDLC +  ++LTVD  SS +AIE FLWP              ++++   K  
Sbjct: 768  RFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVTRKKAEQSSEADSLREHQVKPL 827

Query: 1773 SK---------EQVGS----------------STTQNNEGDIT-------PKLIFLLDGK 1856
            S          E VGS                ST + ++G  +       PKL+  L+GK
Sbjct: 828  SNVCSYFGVNPELVGSNSKSSDLPEIEVSVEVSTDEKSQGSASSSKKGTKPKLLLYLEGK 887

Query: 1857 QLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTL 2036
            QL+ ++TLYQ ILQ  I E+ + I   K W++V+ +  +SA        ++  + C    
Sbjct: 888  QLEPTLTLYQTILQQHIKEN-EAISGTKVWSQVYTIMYKSA-------GEVEDNSCNQFF 939

Query: 2037 SWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERS 2216
            S        H + SFF  +L  +LP  L + +P YD+LF+L+I+EG+NR++F + S+ER 
Sbjct: 940  SASDKGATLHFS-SFFCGILDCDLPSDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERI 998

Query: 2217 NAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVA 2396
             AF+EG +   +++K+   S+ Q EF+NSKLT KLEQQM D  A+S   MPLWC +L+ +
Sbjct: 999  RAFSEGRISTLNNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDS 1058

Query: 2397 CPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNIL 2576
            CPFLFSFEAR KYF + +FG+ + Q +     ++ T N  RS S   PRKK  V RN IL
Sbjct: 1059 CPFLFSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGFPRKKALVLRNQIL 1117

Query: 2577 ASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAK 2756
             SAAKMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  FQK G+GMWRGDH +   
Sbjct: 1118 LSAAKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP 1177

Query: 2757 RLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRI 2936
                S+ +     I    GLFPRPW + +D    + FS+V+K F L+GQ+ A+AIQDGR+
Sbjct: 1178 --GKSLNIEGRETIESPFGLFPRPWLSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRV 1234

Query: 2937 LDLPFSRAFYKLMLEQDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFD 3116
            +D+ FS+AFYKL+L Q++ IYDIQSFDPELG  ++EFQAL  R   LES   E+S  + +
Sbjct: 1235 MDIYFSKAFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSKLE 1294

Query: 3117 PIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLE 3296
              + +T IE+LCLDF+LPGYPD   +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E
Sbjct: 1295 FCYHDTNIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIE 1354

Query: 3297 SFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFL 3476
            +FKSGF++VFPI+ LQ+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S   +  L
Sbjct: 1355 AFKSGFNQVFPIEHLQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLL 1414

Query: 3477 EIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANY 3656
            EIIQ+FD  Q+RAFL FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMTCANY
Sbjct: 1415 EIIQDFDNEQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANY 1474

Query: 3657 LKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
            LKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1475 LKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1504


>ref|XP_022983499.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita maxima]
 ref|XP_022983500.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita maxima]
          Length = 1514

 Score = 1001 bits (2588), Expect = 0.0
 Identities = 570/1290 (44%), Positives = 800/1290 (62%), Gaps = 42/1290 (3%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++N+CK++P +C   +++AVP+LCNL  + D +LVE+ A C+++I E
Sbjct: 242  FFSSSIQRTALRTVVNICKKLPSECPLTLIEAVPILCNLFQHDDGELVENVARCMIKIAE 301

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +LV+L++ S+++ ++LY
Sbjct: 302  CAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLY 361

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E +I+N L+ IL   +LS         D  R+QV   LKLLN+L+PT   +D   + +  
Sbjct: 362  ELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSE 418

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            K   +   PK   +F + ILP L+Q V+SGA   +   C+++I  ++ +   DML  L++
Sbjct: 419  KVSFLAINPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQ 478

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKEGV  AI  L   +  
Sbjct: 479  NANISSFLVGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEKY 538

Query: 903  YQ--SQLMDQQSNNLGDTDERTAADLSRCLCYAFDQSS-SPSSESKMCNLPRHTLLPLAQ 1073
             Q    +      + G   + T  +  RCLCYAF     S  +E+  C L + ++  LA 
Sbjct: 539  KQLIFPVFAGVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDKDSVYSLAN 597

Query: 1074 HVMSTFF-XXXXXXXXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAV 1244
            H+ + +F               Q L     ALD   N+       A+ E+    ++ E +
Sbjct: 598  HIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYTLLAEIM 657

Query: 1245 TALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALD-SDMSFDYLTVLKRLQTFSFTL 1421
            + L  GE ISTFEFIESG ++   +YLTNG YL +  +   +S  +  + +R + F+  L
Sbjct: 658  SKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLL 717

Query: 1422 LSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQV 1595
             S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++    +P      HPC++V
Sbjct: 718  FSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKV 777

Query: 1596 HFVAEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVD 1772
             FV  +GETDLC +  ++LTVD  SS +AIE FLWP              ++++   K  
Sbjct: 778  RFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVTRKKAEQSSEADSLREHQVKPL 837

Query: 1773 SK---------EQVGS----------------STTQNNEGDIT-------PKLIFLLDGK 1856
            S          E VGS                ST + ++G  +       PKL+  L+GK
Sbjct: 838  SNVCSYFGVNPELVGSNSKSSDLPEIEVSVEVSTDEKSQGSASSSKKGTKPKLLLYLEGK 897

Query: 1857 QLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTL 2036
            QL+ ++TLYQ ILQ  I E+ + I   K W++V+ +  +SA        ++  + C    
Sbjct: 898  QLEPTLTLYQTILQQHIKEN-EAISGTKVWSQVYTIMYKSA-------GEVEDNSCNQFF 949

Query: 2037 SWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERS 2216
            S        H + SFF  +L  +LP  L + +P YD+LF+L+I+EG+NR++F + S+ER 
Sbjct: 950  SASDKGATLHFS-SFFCGILDCDLPSDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERI 1008

Query: 2217 NAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVA 2396
             AF+EG +   +++K+   S+ Q EF+NSKLT KLEQQM D  A+S   MPLWC +L+ +
Sbjct: 1009 RAFSEGRISTLNNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDS 1068

Query: 2397 CPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNIL 2576
            CPFLFSFEAR KYF + +FG+ + Q +     ++ T N  RS S   PRKK  V RN IL
Sbjct: 1069 CPFLFSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGFPRKKALVLRNQIL 1127

Query: 2577 ASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAK 2756
             SAAKMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  FQK G+GMWRGDH +   
Sbjct: 1128 LSAAKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP 1187

Query: 2757 RLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRI 2936
                S+ +     I    GLFPRPW + +D    + FS+V+K F L+GQ+ A+AIQDGR+
Sbjct: 1188 --GKSLNIEGRETIESPFGLFPRPWLSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRV 1244

Query: 2937 LDLPFSRAFYKLMLEQDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFD 3116
            +D+ FS+AFYKL+L Q++ IYDIQSFDPELG  ++EFQAL  R   LES   E+S  + +
Sbjct: 1245 MDIYFSKAFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSKLE 1304

Query: 3117 PIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLE 3296
              + +T IE+LCLDF+LPGYPD   +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E
Sbjct: 1305 FCYHDTNIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIE 1364

Query: 3297 SFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFL 3476
            +FKSGF++VFPI+ LQ+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S   +  L
Sbjct: 1365 AFKSGFNQVFPIEHLQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLL 1424

Query: 3477 EIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANY 3656
            EIIQ+FD  Q+RAFL FVTGAPRLP GG A+L PKLTIV KH  N  D +LPSVMTCANY
Sbjct: 1425 EIIQDFDNEQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANY 1484

Query: 3657 LKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
            LKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1485 LKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1514


>ref|XP_022934794.1| E3 ubiquitin-protein ligase UPL4-like isoform X2 [Cucurbita moschata]
          Length = 1504

 Score =  995 bits (2573), Expect = 0.0
 Identities = 565/1290 (43%), Positives = 796/1290 (61%), Gaps = 42/1290 (3%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++N+CK++P +C   +++AVP+LCNL  + D +LVE+ A C+++I E
Sbjct: 232  FFSSSIQRTALRTVVNICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAE 291

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +LV+L++ S+++ ++LY
Sbjct: 292  CAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLY 351

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E +I+N L+ IL   +LS         D  R+QV   LKLLN+L+PT   +D   + +  
Sbjct: 352  ELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSE 408

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            K   +   PK   +F + ILP L+Q V+SGA   +   C+++I  ++ +   DML  L++
Sbjct: 409  KVSFLASNPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQ 468

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKEGV  AI  L   +  
Sbjct: 469  NANISSFLVGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEKY 528

Query: 903  YQ--SQLMDQQSNNLGDTDERTAADLSRCLCYAFDQSS-SPSSESKMCNLPRHTLLPLAQ 1073
             Q    +      + G   + T  +  RCLCYAF     S  +E+  C L   ++  LA 
Sbjct: 529  KQLIFPVFASVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLAN 587

Query: 1074 HVMSTFF-XXXXXXXXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAV 1244
            H+ + +F               Q L     ALD   N+        + E+    ++ E +
Sbjct: 588  HIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTPGQDEEKFYALLAEIM 647

Query: 1245 TALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALD-SDMSFDYLTVLKRLQTFSFTL 1421
            + L  GE ISTFEFIESG ++   +YLTNG YL +  +   +S  +  + +R + F+  L
Sbjct: 648  SKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLL 707

Query: 1422 LSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQV 1595
             S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++    +P      HPC++V
Sbjct: 708  FSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKV 767

Query: 1596 HFVAEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVD 1772
             FV  +GETDLC +  ++LTVD  SS +AIE FLWP              ++++   K+ 
Sbjct: 768  RFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLL 827

Query: 1773 SK---------EQVGSSTTQNNEGDI-----------------------TPKLIFLLDGK 1856
            S          E VGS++  ++  +I                        PKL+  L+GK
Sbjct: 828  SNVCSYSGVNPELVGSNSKSSDLPEIEASVEVSTDEKSLGSASSSKKGTKPKLLLYLEGK 887

Query: 1857 QLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTL 2036
            QL+ ++TLYQ ILQ  I E+ + I   K W++V+ +  + A        ++  + C    
Sbjct: 888  QLEPTLTLYQTILQRHIKEN-EAISGTKVWSQVYTIMYKRA-------GEVEDNSCNQFF 939

Query: 2037 SWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERS 2216
            S        H + SFF  +L  +LP  L + +P YD+LF+L+I+EG+NR++F + S+ER 
Sbjct: 940  SGSDKGATLHFS-SFFCGILDCDLPSDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERI 998

Query: 2217 NAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVA 2396
             AF+EG +   D++K+   S+ Q EF+NSKLT KLEQQM D  A+S   MPLWC +L+ +
Sbjct: 999  RAFSEGRISTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDS 1058

Query: 2397 CPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNIL 2576
            CPFLFSFEAR KYF + +FG+ + Q +     ++ T N  RS S  LPRKK  V R+ IL
Sbjct: 1059 CPFLFSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGLPRKKALVLRDKIL 1117

Query: 2577 ASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAK 2756
             SAAKMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  FQK G+GMWRGDH +   
Sbjct: 1118 LSAAKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP 1177

Query: 2757 RLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRI 2936
                S+ +     I    GLFPRPW + +D    + FS+V+K F L+GQ+ A+AIQDGR+
Sbjct: 1178 --GKSLNIEGRETIESPFGLFPRPWLSTVDIGE-LQFSEVIKRFTLMGQIVAKAIQDGRV 1234

Query: 2937 LDLPFSRAFYKLMLEQDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFD 3116
            +D+ FS+AFYKL+L Q++ IYDIQSFDPELG  ++EFQAL  R   LES   E+S  + +
Sbjct: 1235 MDIYFSKAFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSKLE 1294

Query: 3117 PIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLE 3296
              + +T IE+LCLDF+LPGYPD   +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E
Sbjct: 1295 FCYHDTNIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIE 1354

Query: 3297 SFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFL 3476
            +FKSGF++VFPI+ LQ+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S   +  L
Sbjct: 1355 AFKSGFNQVFPIEHLQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLL 1414

Query: 3477 EIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANY 3656
            EIIQ+FD  Q+RAFL FVTGAPRLP GG  +L PKLTIV KH  N  D +LPSVMTCANY
Sbjct: 1415 EIIQDFDNEQQRAFLQFVTGAPRLPSGGFTSLNPKLTIVRKHSSNMVDSDLPSVMTCANY 1474

Query: 3657 LKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
            LKLPPYSSKE  +EKLLYAI EGQG+FHLS
Sbjct: 1475 LKLPPYSSKEITKEKLLYAITEGQGSFHLS 1504


>ref|XP_022934791.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita moschata]
 ref|XP_022934792.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita moschata]
          Length = 1514

 Score =  995 bits (2573), Expect = 0.0
 Identities = 565/1290 (43%), Positives = 796/1290 (61%), Gaps = 42/1290 (3%)
 Frame = +3

Query: 3    FFELSAQRIALSTIINVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVE 182
            FF  S QR AL T++N+CK++P +C   +++AVP+LCNL  + D +LVE+ A C+++I E
Sbjct: 242  FFSSSIQRTALRTVVNICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAE 301

Query: 183  NFGHSSELLDELCKHGVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLY 362
                S E+LD LC+HG+++Q ++LI+ +S  +LS+T Y  ++ +LV+L++ S+++ ++LY
Sbjct: 302  CAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLY 361

Query: 363  ESNINNILESILRNPDLSRRTPPSHCEDISRDQVYVALKLLNQLIPTVARDDQDIQLVLG 542
            E +I+N L+ IL   +LS         D  R+QV   LKLLN+L+PT   +D   + +  
Sbjct: 362  ELDISNTLKEILSVYNLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSE 418

Query: 543  KEKIIIDEPKLSFQFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIK 722
            K   +   PK   +F + ILP L+Q V+SGA   +   C+++I  ++ +   DML  L++
Sbjct: 419  KVSFLASNPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQ 478

Query: 723  NANMPKFLAGLLSRKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGC 902
            NAN+  FL G+ +RKDHHVL+ AL + EII++KL    L SFVKEGV  AI  L   +  
Sbjct: 479  NANISSFLVGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEKY 538

Query: 903  YQ--SQLMDQQSNNLGDTDERTAADLSRCLCYAFDQSS-SPSSESKMCNLPRHTLLPLAQ 1073
             Q    +      + G   + T  +  RCLCYAF     S  +E+  C L   ++  LA 
Sbjct: 539  KQLIFPVFASVHPSFGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLAN 597

Query: 1074 HVMSTFF-XXXXXXXXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAV 1244
            H+ + +F               Q L     ALD   N+        + E+    ++ E +
Sbjct: 598  HIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTPGQDEEKFYALLAEIM 657

Query: 1245 TALNEGESISTFEFIESGFIRFLAHYLTNGDYLHRALD-SDMSFDYLTVLKRLQTFSFTL 1421
            + L  GE ISTFEFIESG ++   +YLTNG YL +  +   +S  +  + +R + F+  L
Sbjct: 658  SKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLL 717

Query: 1422 LSG--HSGQHFPLASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQV 1595
             S   H   + P+  L++ LQ +LS+LE FPV+ S   K ++    +P      HPC++V
Sbjct: 718  FSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKV 777

Query: 1596 HFVAEEGETDLC-LYDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVD 1772
             FV  +GETDLC +  ++LTVD  SS +AIE FLWP              ++++   K+ 
Sbjct: 778  RFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLL 837

Query: 1773 SK---------EQVGSSTTQNNEGDI-----------------------TPKLIFLLDGK 1856
            S          E VGS++  ++  +I                        PKL+  L+GK
Sbjct: 838  SNVCSYSGVNPELVGSNSKSSDLPEIEASVEVSTDEKSLGSASSSKKGTKPKLLLYLEGK 897

Query: 1857 QLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTL 2036
            QL+ ++TLYQ ILQ  I E+ + I   K W++V+ +  + A        ++  + C    
Sbjct: 898  QLEPTLTLYQTILQRHIKEN-EAISGTKVWSQVYTIMYKRA-------GEVEDNSCNQFF 949

Query: 2037 SWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERS 2216
            S        H + SFF  +L  +LP  L + +P YD+LF+L+I+EG+NR++F + S+ER 
Sbjct: 950  SGSDKGATLHFS-SFFCGILDCDLPSDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERI 1008

Query: 2217 NAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVA 2396
             AF+EG +   D++K+   S+ Q EF+NSKLT KLEQQM D  A+S   MPLWC +L+ +
Sbjct: 1009 RAFSEGRISTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDS 1068

Query: 2397 CPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNIL 2576
            CPFLFSFEAR KYF + +FG+ + Q +     ++ T N  RS S  LPRKK  V R+ IL
Sbjct: 1069 CPFLFSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGLPRKKALVLRDKIL 1127

Query: 2577 ASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHVFQKVGMGMWRGDHTSTAK 2756
             SAAKMM  YA +KVLLE+E+  EVGTGLGPTLEFYTLVS  FQK G+GMWRGDH +   
Sbjct: 1128 LSAAKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP 1187

Query: 2757 RLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRI 2936
                S+ +     I    GLFPRPW + +D    + FS+V+K F L+GQ+ A+AIQDGR+
Sbjct: 1188 --GKSLNIEGRETIESPFGLFPRPWLSTVDIGE-LQFSEVIKRFTLMGQIVAKAIQDGRV 1244

Query: 2937 LDLPFSRAFYKLMLEQDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFD 3116
            +D+ FS+AFYKL+L Q++ IYDIQSFDPELG  ++EFQAL  R   LES   E+S  + +
Sbjct: 1245 MDIYFSKAFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSKLE 1304

Query: 3117 PIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLE 3296
              + +T IE+LCLDF+LPGYPD   +S  D+ MVN  NLE+YVSLV DAT+ SGIS Q+E
Sbjct: 1305 FCYHDTNIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIE 1364

Query: 3297 SFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFL 3476
            +FKSGF++VFPI+ LQ+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S   +  L
Sbjct: 1365 AFKSGFNQVFPIEHLQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLL 1424

Query: 3477 EIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANY 3656
            EIIQ+FD  Q+RAFL FVTGAPRLP GG  +L PKLTIV KH  N  D +LPSVMTCANY
Sbjct: 1425 EIIQDFDNEQQRAFLQFVTGAPRLPSGGFTSLNPKLTIVRKHSSNMVDSDLPSVMTCANY 1484

Query: 3657 LKLPPYSSKERMREKLLYAIREGQGAFHLS 3746
            LKLPPYSSKE  +EKLLYAI EGQG+FHLS
Sbjct: 1485 LKLPPYSSKEITKEKLLYAITEGQGSFHLS 1514